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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 649 records · Page 36
All sourcesNASA NTRSDOE OSTI
DOE OSTI2020

Switchable resolution in soft x-ray tomography of single cells

The diversity of living cells, in both size and internal complexity, calls for imaging methods with adaptable spatial resolution. Soft x-ray tomography (SXT) is a three-dimensional imaging technique ideally suited to visualizing and quantifying the internal organization of single cells of varying sizes in a near-native state. The achievable resolution of the soft x-ray microscope is largely determined by the objective lens, but switching between objectives is extremely time-consuming and typically undertaken only during microscope maintenance procedures. Since the resolution of the optic is inversely proportional to the depth of focus, an optic capable of imaging the thickest cells is routinely selected. This unnecessarily limits the achievable resolution in smaller cells and eliminates the ability to obtain high-resolution images of regions of interest in larger cells. Here, we describe developments to overcome this shortfall and allow selection of microscope optics best suited to the specimen characteristics and data requirements. We demonstrate that switchable objective capability advances the flexibility of SXT to enable imaging cells ranging in size from bacteria to yeast and mammalian cells without physically modifying the microscope, and we demonstrate the use of this technology to image the same specimen with both optics.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2021

Accurate in vivo tumor detection using plasmonic-enhanced shifted-excitation Raman difference spectroscopy (SERDS)

For the majority of cancer patients, surgery is the primary method of treatment. In these cases, accurately removing the entire tumor without harming surrounding tissue is critical; however, due to the lack of intraoperative imaging techniques, surgeons rely on visual and physical inspection to identify tumors. Surface-enhanced Raman scattering (SERS) is emerging as a non-invasive optical alternative for intraoperative tumor identification, with high accuracy and stability. However, Raman detection requires dark rooms to work, which is not consistent with surgical settings. Methods: Herein, we used SERS nanoprobes combined with shifted-excitation Raman difference spectroscopy (SERDS) detection, to accurately detect tumors in xenograft murine model. Results: We demonstrate for the first time the use of SERDS for in vivo tumor detection in a murine model under ambient light conditions. We compare traditional Raman detection with SERDS, showing that our method can improve sensitivity and accuracy for this task. Conclusion: Our results show that this method can be used to improve the accuracy and robustness of in vivo Raman/SERS biomedical application, aiding the process of clinical translation of these technologies.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗
NASA NTRS1967

The analysis of Gemini 7 EEG data by three different computer oriented methods

The problem in assessment of electroencephalograms has been the subject element in visual evaluations of such records as well as the need for extensive training (2 or 3 years) before suitable candidate is capable to perform this task. There have been variety of computer type analyses of EEG during the past ten years or more, and we are still searching for a reliable, inexpensive, online data processing procedure for this task. We are aware our Soviet colleagues are routinely using computer assessment of EEGs, using relatively inexpensive hardware. In our report we endeavor to show how four different assessment procedures (visual assessment, zero crossing technique, smoothing and peak counting technique, and Weibull Statistic) may be utilized, and indicate the advantages and disadvantages peculiar to each. We wish to point out that in the Gemini 7 EEG analog records supplied to the Henry Ford Hospital, Channel II (mid-line central to mid-line occipital electrodes) could not be assessed reliably after approximately 26 hours of flight. Channel I (left central to left occipital electrodes) provided the more assessable EEG up to approximately 54 hours of flight. These channels were compared during the first 26 hours of flight, and no significant difference was found by our various assessment procedures. We, therefore, have limited this report to the findings on the analyses of Channel I during its approximate 54 hours of flight.

Proctor, L. D.↗
NASA NTRS1973

Remote sensing applied to land-use studies in Wyoming

Impending development of Wyoming's vast fuel resources requires a quick and efficient method of land use inventory and evaluation. Preliminary evaluations of ERTS-1 imagery have shown that physiographic and land use inventory maps can be compiled by using a combination of visual and automated interpretation techniques. Test studies in the Powder River Basin showed that ERTS image interpretations can provide much of the needed physiographic and land use information. Water impoundments as small as one acre were detected and water bodies larger than five acres could be mapped and their acreage estimated. Flood plains and irrigated lands were successfully mapped, and some individual crops were identified and mapped. Coniferous and deciduous trees were mapped separately using color additive analysis on the ERTS multispectral imagery. Gross soil distinctions were made with the ERTS imagery, and were found to be closely related to the bedrock geology. Several broad unstable areas were identified. These were related to specific geologic and slope conditions and generally extended through large regions. Some new oil fields and all large open-cut coal mines were mapped. The most difficult task accomplished was that of mapping urban areas. Work in the urban areas provides a striking example of snow enhancement and the detail available from a snow enhanced image.

Breckenridge, R. M.↗
NASA NTRS1972

Automatic surface inoculation of agar trays.

Description of a machine and technique for the automatic inoculation of a plastic tray containing agar media with a culture, using either a conventional inoculation loop or a cotton swab. The design of the machine is simple, it is easy to use, and it relieves the operator from the manual task of streaking cultures. The described technique makes possible the visualization of the overall qualitative and, to some extent, quantitative relationships of various bacterial types in a sample tested.

Wilkins, J. R.↗
NASA NTRS1976

Protection coordination of the Kennedy Space Center electric distribution network

A computer technique is described for visualizing the coordination and protection of any existing system of devices and settings by plotting the tripping characteristics of the involved devices on a common basis. The program determines the optimum settings of a given set of protective devices and configuration in the sense of the best expected coordinated operation of these devices. Subroutines are given for simulating time versus current characteristics of the different relays, circuit breakers, and fuses in the system; coordination index computation; protection checks; plotting; and coordination optimation.

Source record↗
NASA NTRS1980

Resonant Doppler velocimeter

A technique is presented for visualizing and quantitatively measuring velocity, temperature, and pressure by shining a single frequency laser beam into a gaseous flow which is seeded with an atomic species. The laser is tuned through the absorption frequencies of the seeded species and the absorption profile is detected by observing fluorescence as the atoms relax back to the ground state. The flow velocity is determined by observing the Doppler shift in the absorption frequency. Spectroscopic absorption line broadening mechanisms furnish information regarding the static temperature and pressure of the moving gas. Results of experiments conducted in the free stream and in the bow shock of a conical model mounted in a hypersonic wind tunnel indicate that the experimental uncertainties in the measurement of average values for the velocity, temperature and pressure of the flow are 0.1, 5 and 10 percent respectively.

Zimmermann, M.↗
NASA NTRS1982

The use of LANDSAT data to monitor the urban growth of Sao Paulo Metropolitan area

Urban growth from 1977 to 1979 of the region between Billings and the Guarapiranga reservoir was mapped and the problematic urban areas identified using several LANDSAT products. Visual and automatic interpretation techniques were applied to the data. Computer compatible tapes of LANDSAT multispectral scanner data were analyzed through the maximum likelihood Gaussian algorithm. The feasibility of monitoring fast urban growth by remote sensing techniques for efficient urban planning and control is demonstrated.

Dejesusparada, N.↗
NASA NTRS1982

Transonic aerodynamics; Transonic Perspective Symposium, Moffett Field, CA, February 18-20, 1981, Technical Papers

After an historical account of the development of transonic aerodynamics before 1940 and in the period 1945-1975, attention is given to the design for cruise performance efficiency of commercial transport aircraft, with emphasis on transonic wing design, the practical aerodynamic problems of military aircraft, with emphasis on store carriage and separation, experimental testing in transonic wind tunnels capable of Reynolds number simulation and aerodynamic flow visualization, and such mathematical techniques as potential equation methods for flow prediction and Reynolds-averaged Navier-Stokes computations of transonic flows. Also considered are transonic design using computational aerodynamics, the application of computation methods to transonic wing design, computational experience with advanced tactical aircraft, the evaluation of full potential flow methods, and the application of a shock-turbulent boundary layer interaction theory to transonic flowfield analysis.

Nixon, D.↗
NASA NTRS1982

Experiments on identification and control of inflow disturbances in contracting streams

Vorticity from all surfaces and isolated objects in the vicinity of the fan intake, including the outside surfaces of the fan housing, were identified as the major sources for disturbances leading to blade passing frequency noise. The previously proposed mechanism based on atmospheric turbulence is refuted. Flow visualization and hot wire techniques were used in three different facilities to document the evolution of various types of disturbances, including the details of the mean flow and turbulence characteristics. The results suggest that special attention must be devoted to the design of the inlet and that geometric modeling may not lead to adequate simulation of the in flight characteristics. While honeycomb type flow manipulators appear to be effective in reducing some of the disturbances, higher pressure drop devices that generate adequate turbulence, for mixing of isolated nonuniformities, may be necessary to suppress the remaining disturbances. The results are also applicable to the design of inlets of open return wind tunnels and similar flow facilities.

Lee, A. H.↗
NASA NTRS1985

Image processing of aerodynamic data

The use of digital image processing techniques in analyzing and evaluating aerodynamic data is discussed. An image processing system that converts images derived from digital data or from transparent film into black and white, full color, or false color pictures is described. Applications to black and white images of a model wing with a NACA 64-210 section in simulated rain and to computed low properties for transonic flow past a NACA 0012 airfoil are presented. Image processing techniques are used to visualize the variations of water film thicknesses on the wing model and to illustrate the contours of computed Mach numbers for the flow past the NACA 0012 airfoil. Since the computed data for the NACA 0012 airfoil are available only at discrete spatial locations, an interpolation method is used to provide values of the Mach number over the entire field.

Faulcon, N. D.↗
NASA NTRS1986

Disturbance functions of the Goertler instability on an airfoil

Goertler vortices arise in boundary layers along concave surfaces due to centrifugal effects. This paper presents some results of an experiment conducted to study the development of these vortices on an airfoil with a pressure gradient in the concave region where an attached laminar boundary layer was insured with suction through a perforated panel. A sublimating chemical technique was used to visualize Goertler vortices and the velocity field was measured by laser velocimetry. Experimental disturbance functions are compared with those predicted by the linear stability theory. The trend of vortex amplification in the concave zone and damping in the following convex region is shown to essentially follow the theoretical predictions.

Dagenhart, J. R.↗
NASA NTRS1986

Analysis of crew functions as an aid in Space Station interior layout

The Space Station must be designed to facilitate all of the functions that its crew will perform, both on-duty and off-duty, as efficiently and comfortably as possible. This paper examines the functions to be performed by the Space Station crew in order to make inferences about the design of an interior layout that optimizes crew productivity. Twenty-seven crew functions were defined, as well as five criteria for assessing relationships among all pairs of those functions. Hierarchical clustering and multidimensional scaling techniques were used to visually summarize the relationships. A key result was the identification of two dimensions for describing the configuration of crew functions: 'Private-Public' and 'Group-Individual'. Seven specific recommendations for Space Station interior layout were derived from the analyses.

Steinberg, A. L.↗
NASA NTRS1985

The Goertler instability on an airfoil

Goertler vortices arise in boundary layers along concave surfaces due to centrifugal effects. This paper presents some results of an experiment conducted to study the development of these vortices on an airfoil with a pressure gradient in the concave region where an attached laminar boundary layer was insured with suction through a perforated panel. The sublimating chemical technique was used to visualize Goertler vortices and the velocity field was measured by laser velocimetry. The vortex wavelength clearly varied with Goertler number as predicted by linear theory. Both flow visualization and velocity measurements indicated vortex damping in the convex zone. Secondary instability was observed at the higher Goertler numbers.

Mangalam, S. M.↗
NASA NTRS1990

Supercomputer applications in gas turbine flowfield simulation

The numerical simulation of the unsteady three-dimensional viscous flow in a gas turbine stage is considered. Results from a three-dimensional time-accurate Navier-Stokes simulation of rotor-stator interaction in an axial turbine stage are presented. The present study uses a fine grid in the spanwise direction to better resolve the complex three-dimensional flowfield, and complements earlier reported coarse-grid calculations. Several different features of the flowfield are analyzed and compared to earlier calculations and to experimental data whenever possible. Computer animation techniques are used to visualize various unsteady three-dimensional features of the flow. The results demonstrate the capabilities of current computing hardware in obtaining accurate simulations of unsteady flows in turbomachines.

Madavan, Nateri K.↗
NASA NTRS1990

Drop coalescence studies

The objective of this experimental study is to understand the detailed mechanics of the coalescence of liquid drops. The experiments are being conducted in an immiscible acoustic levitator with degassed water as the host medium. Typically, a quasineutrally buoyant drop of silicon oil mixed with bromobenzene is levitated close to the velocity node of the levitator. A second drop of the same liquid is introduced, and as it slowly seeks levitation position, the drops coalesce. Coalescence is delayed until the host film between drops is completely drained. Following coalescence, the excess surface energy in the coalesced drop is dissipated through shape oscillations. The final events of film rupture followed by drop coalescence are rapid and are photographically studied with high-speed video (1000 fps). The laser-induced fluorescence technique is used to visualize the dynamics of host film drainage. The details of the coalescence mechanics are presented.

Anikumar, A. V.↗
NASA NTRS1992

Characteristics of two adjacent rectangular jets

Rectangular supersonic (Design Mach number 1.8), single and twin free jets were tested in a Mach number range of 1.2 to 2.4. The objective was to study the interaction between the jets and the effect of the interaction on the spreading rate of the jets. Nozzle spacing in the range of one to four equivalent exit diameters, and jet Mach number were the parameters investigated. The experimental techniques used were Schlieren visualization and direct mixing (gas sampling) measurements from which jet spreading can be readily obtained. At the design Mach number of the nozzles and above the twin jets interact but the interaction does not affect the spreading rate of the jets. At lower Mach numbers, a moderate increase in the twin jets' spread compared to the single jet's spread was observed. The greater increase was observed for nozzle spacing of two equivalent exit diameters. Similar conclusions are drawn from both visualization and direct mixing results.

Zeierman, S.↗