Chemical variations in Yellowknife Bay formation sedimentary rocks analyzed by ChemCam on board the Curiosity rover on Mars
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AGU Fall Meeting 2022, Chicago, IL, December 12-16, 2022
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For presentation at the 10th International Conference on Gas Hydrates, Singapore, July 10-14, 2023.
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Besides long-term average temperature increases, climate change is projected to result in a higher frequency of marine heatwaves. Coastal zones are some of the most productive and vulnerable ecosystems, with many stretches already under anthropogenic pressure. Microorganisms in coastal areas are central to marine energy and nutrient cycling and therefore, it is important to understand how climate change will alter these ecosystems. Using a long-term heated bay (warmed for 50 years) in comparison with an unaffected adjacent control bay and an experimental short-term thermal (9 days at 6–35 °C) incubation experiment, this study provides new insights into how coastal benthic water and surface sediment bacterial communities respond to temperature change. Benthic bacterial communities in the two bays reacted differently to temperature increases with productivity in the heated bay having a broader thermal tolerance compared with that in the control bay. Furthermore, the transcriptional analysis showed that the heated bay benthic bacteria had higher transcript numbers related to energy metabolism and stress compared to the control bay, while short-term elevated temperatures in the control bay incubation experiment induced a transcript response resembling that observed in the heated bay field conditions. In contrast, a reciprocal response was not observed for the heated bay community RNA transcripts exposed to lower temperatures indicating a potential tipping point in community response may have been reached. In summary, long-term warming modulates the performance, productivity, and resilience of bacterial communities in response to warming.
Harmful algal blooms are increasing in tropical estuaries which can have complex morphologies and hydrologic regimes while being less well studied than temperate estuaries. Spatial and temporal patterns of algal bloom occurrence in Florida Bay were examined to evaluate the potential contribution of the various freshwater inputs to the subtropical bay as nutrient sources. Monthly water quality data, from 1989 to 2009, at 28 sampling stations across the bay were analyzed at the station-month level, aggregated into hydrologic Zones of Similar Influence and based on annual rainfall seasons. The Zones of Similar Influence are linked to the geomorphology of the bay with western areas being more directly connected to the Southwest Florida Shelf waters than eastern areas. Correlation analysis suggested that inputs of phosphorus were the predominant factor in the initiation of elevated chlorophyll a (chl-a) levels but was also consistent with higher nitrogen limitation in western Florida Bay as reported in literature. Differences in mean monthly chl-a indicated a seasonality of algal blooms with elevated chl-a concentrations following heavy precipitation months for stations in the north-central and western areas of the bay where algal blooms have been re-occurring. Differences in stations' chl-a concentrations showed stations to the northwest as having significantly higher concentrations than more interior stations during the dry season but not during the rainy season (when algal blooms are occurring). Mapping the sampling stations atop the bathymetry of Florida Bay highlighted the importance of coastal morphology in evaluation of potential nutrient pathways for estuarine algal bloom sources. The specific factors resulting in the seasonal cycles of blooms remained unresolved but portions of the bay and times of year were identified as important areas for further research. This study indicates that illustrating the interplay of geomorphology and winds and rains at fine temporal and spatial resolution is required to describe nutrient circulation for systems with complex morphologies such as those associated with reefs, island matrices and headlands.
An extension boom is to be installed on the starboard side of the Space Shuttle Orbiter (SSO) payload bay for thermal tile inspection and repairing. As a result, the Space Shuttle payload bay Ultra High Frequency (UHF) antenna will be under the boom. This study is to evaluate the Space Shuttle UHF communication performance for antenna at a suitable new location. To insure the RF coverage performance at proposed new locations, the link margin between the UHF payload bay antenna and Extravehicular Activity (EVA) Astronauts at a range distance of 160 meters from the payload bay antenna was analyzed. The communication performance between Space Shuttle Orbiter and International Space Station (SSO-ISS) during rendezvous was also investigated. The multipath effects from payload bay structures surrounding the payload bay antenna were analyzed. The computer simulation tool based on the Geometrical Theory of Diffraction method (GTD) was used to compute the signal strengths. The total field strength was obtained by summing the direct fields from the antennas and the reflected and diffracted fields from the surrounding structures. The computed signal strengths were compared to the signal strength corresponding to the 0 dB link margin. Based on the results obtained in this study, RF coverage for SSO-EVA and SSO- ISS communication links was determined for the proposed payload bay antenna UHF locations. The RF radiation to the Orbiter Docking System (ODS) pyros, the payload bay avionics, and the Shuttle Remote Manipulator System (SRMS) from the new proposed UHF antenna location was also investigated to ensure the EMC/EMI compliances.
Recent advances in satellite and airborne remote sensing, such as improvements in sensor and algorithm calibrations, processing techniques and atmospheric correction procedures have provided for increased coverage of remote-sensing, ocean-color products for coastal regions. In particular, for the Moderate Resolution Imaging Spectrometer (MODIS) sensor calibration updates, improved aerosol retrievals and new aerosol models has led to improved atmospheric correction algorithms for turbid waters and have improved the retrieval of ocean color in coastal waters. This has opened the way for studying ocean phenomena and processes at finer spatial scales, such as the interactions at the land-sea interface, trends in coastal water quality and algal blooms. Human population growth and changes in coastal management practices have brought about significant changes in the concentrations of organic and inorganic, particulate and dissolved substances entering the coastal ocean. There is increasing concern that these inputs have led to declines in water quality and have increase local concentrations of phytoplankton, which cause harmful algal blooms. In two case studies we present MODIS observations of fluorescence line height (FLH) to 1) assess trends in water quality for Tampa Bay, Florida and 2) illustrate seasonal and annual variability of algal bloom activity in Monterey Bay, California as well as document estuarine/riverine plume induced red tide events. In a comprehensive analysis of long term (2003-2011) in situ monitoring data and satellite imagery from Tampa Bay we assess the validity of the MODIS FLH product against chlorophyll-a and a suite of water quality parameters taken in a variety of conditions throughout a large optically complex estuarine system. A systematic analysis of sampling sites throughout the bay is undertaken to understand how the relationship between FLH and in situ chlorophyll-a responds to varying conditions and to develop a near decadal trend in water quality changes. In situ monitoring locations that correlated well with satellite imagery were in depths greater than seven meters and were located over five kilometers from shore. Water quality parameter of total nitrogen, phosphorous, turbidity and biological oxygen demand had high correlations with these sites, as well. Satellite FLH estimates show improving water quality from 2003-2007 with a slight decline up through 2011. Dinoflagellate blooms in Monterey Bay, California (USA) have recently increased in frequency and intensity. Nine years of MODIS FLH observations are used to describe the annual and seasonal variability of bloom activity within the Bay. Three classes of MODIS algorithms were correlated against in situ chlorophyll measurements. The FLH algorithm provided the most robust estimate of bloom activity. Elevated concentrations of phytoplankton were evident during the months of August-November, a period during which increased occurrences of dinoflagellate blooms have been observed in situ. Seasonal patterns of FLH show the on- and offshore movement of areas of high phytoplankton biomass between oceanographic seasons. Higher concentrations of phytoplankton are also evident in the vicinity of the land-based nutrient sources and outflows, and the cyclonic bay-wide circulation can transport these nutrients to the northern Bay bloom incubation region. Both of these case studies illustrate the utility MODIS FLH observations in supporting management decisions in coastal and estuarine waters.
Climate change related warming is a serious environmental problem attributed to anthropogenic activities, causing ocean water temperatures to rise in the coastal marine ecosystem since the last century. This particularly affects benthic microbial communities, which are crucial for biogeochemical cycles. While bacterial communities have received considerable scientific attention, the benthic eukaryotic community response to climate change remains relatively overlooked. In this study, sediments were sampled from a heated (average 5°C increase over the whole year for over 50 years) and a control (contemporary conditions) Baltic Sea bay during four different seasons across a year. RNA transcript counts were then used to investigate eukaryotic community changes under long-term warming. The composition of active species in the heated and control bay sediment eukaryotic communities differed, which was mainly attributed to salinity and temperature. The family level RNA transcript alpha diversity in the heated bay was higher during May but lower in November, compared with the control bay, suggesting altered seasonal activity patterns and dynamics. In addition, structures of the active eukaryotic communities varied between the two bays during the same season. Hence, this study revealed that long-term warming can change seasonality in eukaryotic diversity patterns. Relative abundances and transcript expression comparisons between bays suggested that some taxa that now have lower mRNA transcripts numbers could be favored by future warming. Furthermore, long-term warming can lead to a more active metabolism in these communities throughout the year, such as higher transcript numbers associated with diatom energy production and protein synthesis in the heated bay during winter. In all, these data can help predict how future global warming will affect the ecology and metabolism of eukaryotic community in coastal sediments.
Viruses are ubiquitous and abundant in the oceans, and viral metagenomes (viromes) have been investigated extensively via several large-scale ocean sequencing projects. However, there have not been any systematic viromic studies in estuaries. Here, we investigated the viromes of the Delaware Bay and Chesapeake Bay, two Mid-Atlantic estuaries. Deep sequencing generated a total of 48,190 assembled viral sequences (>5 kb) and 26,487 viral populations (9,204 virus clusters and 17,845 singletons), including 319 circular viral contigs between 7.5 kb and 161.8 kb. Unknown viruses represented the vast majority of the dominant populations, while the composition of known viruses, such as pelagiphage and cyanophage, appeared to be relatively consistent across a wide range of salinity gradients and in different seasons. A difference between estuarine and ocean viromes was reflected by the proportions of Myoviridae, Podoviridae, Siphoviridae, Phycodnaviridae, and a few well-studied virus representatives. The difference in viral community between the Delaware Bay and Chesapeake Bay is significantly more pronounced than the difference caused by temperature or salinity, indicating strong local profiles caused by the unique ecology of each estuary. Interestingly, a viral contig similar to phages infecting Acinetobacter baumannii (“Iraqibacter”) was found to be highly abundant in the Delaware Bay but not in the Chesapeake Bay, the source of which is yet to be identified. Highly abundant viruses in both estuaries have close hits to viral sequences derived from the marine single-cell genomes or long-read single-molecule sequencing, suggesting that important viruses are still waiting to be discovered in the estuarine environment.
Coastal waters such as those found in the Baltic Sea already suffer from anthropogenic related problems including increased algal blooming and hypoxia while ongoing and future climate change will likely worsen these effects. Microbial communities in sediments play a crucial role in the marine energy- and nutrient cycling, and how they are affected by climate change and shape the environment in the future is of great interest. The aims of this study were to investigate potential effects of prolonged warming on microbial community composition and nutrient cycling including sulfate reduction in surface (∼0.5 cm) to deeper sediments (∼ 24 cm). To investigate this, 16S rRNA gene amplicon sequencing was performed, and sulfate concentrations were measured and compared between sediments in a heated bay (which has been used as a cooling water outlet from a nearby nuclear power plant for approximately 50 years) and a nearby but unaffected control bay. The results showed variation in overall microbial diversity according to sediment depth and higher sulfate flux in the heated bay compared to the control bay. A difference in vertical community structure reflected increased relative abundances of sulfur oxidizing- and sulfate reducing bacteria along with a higher proportion of archaea, such as Bathyarchaeota, in the heated compared to the control bay. This was particularly evident closer to the sediment surface, indicating a compression of geochemical zones in the heated bay. These results corroborate findings in previous studies and additionally point to an amplified effect of prolonged warming deeper in the sediment, which could result in elevated concentrations of toxic compounds and greenhouse gases closer to the sediment surface.
The Space Station-based Customer Servicing Facility service bay requirements for service accommodation to the Initial Orbit Capability (IOC) and far-term Station Accommodation Test Sets (SETS) missions are analyzed using the developed mission traffic model. Analysis results are presented which indicate that one servicing bay will be sufficient to accommodate IOC customer servicing requirements. Growth servicing requirements indicate that an additional servicing bay will be needed for accommodation of the far-term SATS mission payloads. Even though the level of total mission accommodation is below 100 percent for one bay at IOC and two bays during growth operations, the levels are such that operational work-around exists so that additional servicing bays will not be required.
In the early 1990's US and Italian scientists collaborated to study the electrodynamics on a long tether between two satellites as it moved through the electrically charged portion of Earth's atmosphere called the ionosphere. Potential uses for the electrical current induced in the long wire include power and thrust generation for a satellite, momentum exchange, artificial gravity, deployment of sensors or antennas, and gravity-gradient stabilization. The Tethered Space Satellite (TSS) was a first-of-its-kind experiment with long tethers in space. It consisted of a satellite with science experiments attached to a 12.5 mile long, very thin (0.10 inch diameter) copper wire assembly wound around a spool in the deployer reel mechanism. The whole mechanism sits on a pallet that is installed into the Shuttle bay. At an altitude of 160 nautical miles above earth, the satellite would be deplodeployed from the Shuttle bay by raising it on a boom facing away from Earth. Once cleared of the bay, the deployer mechanism would slowly feed out the 12-plus miles of tether. Scientific data would be collected throughout the operation, after which the satellite would be reeled back in. A receiver spool to catch the 12.5 mile tether as it was being unwound by the deployer reel mechanism was set up to do the system-level test of deployer real mechanism prior to installing the loaded pallet into the Shuttle bay. The system level tests were required before the pallet could be installed into the Space Shuttle cargo bay. A few months before flight, the system level tests, including unreeling and reeling the tether, were completed at Kennedy Space Center (KSC) and the TSS payload was installed onto the Spacelab pallet. Some of this testing equipment was then shipped back to the contractor, Martin Marietta. Integration with the Shuttle began. Systems-level load analyses, which cannot be run until all information about each payload is finalized, was run in parallel with the physical integration of the hardware into the Shuttle payload bay. An analysis, called Coupled loads analysis, incorporates any updates to the model due to system level tests of all the different payloads, and any changes that were found during integration. Engineering analysis examines the worst case scenarios for the loads the hardware will see. The two times during the mission where the dynamic loads are the worst were 1) the first 10-second portion of Shuttle lift off, and 2) a 2-second time during landing when the landing gears hit the ground. The coupled loads analysis using the final verification loads showed that a single bolt attaching the deployer reel mechanism to the support structure had a "negative margin" - which is an indication that it might fail - during touch down. Hardware certification rules do not allow for hardware to fly with negative margins. A structural failure of one payload could have serious or catastrophic consequences to other payloads, or may significantly damage the Orbiter. The issue had to be resolved before the flight.
This paper presents risk-based explosion analyses used to determine if an explosion of NASA's Space Launch System (SLS) Exploration Upper Stage (EUS) during fueling in Vehicle Assembly Building (VAB) High Bay 4 can hazard the Space Launch System (SLS) being processed in Bay 3. This paper focuses on blast fragmentation as it was the controlling hazard. An estimate of the amount of Net Explosion Weight (NEW) was used to develop a fragment list (mass, shape, takeoff velocity/angle) given an explosion occurs at ground level in Bay 4. Intervening superstructure that exists between Bay 4 and Bay 3 (that could potentially block EUS fragments) was modeled based on detailed visual images (VISSIM) and AUTOCAD/structural drawings. The fragment list was input to a 3D trajectory application that performs multiple random explosion simulations; each simulation varying fragment ballistic coefficient and takeoff angle/velocity. Individual fragments are tracked until stopped by the intervening superstructure or reach Bay 3, potentially impacting the SLS. The probability of impacting the SLS is then determined from the random simulations; the results are used to help decision-makers determine the efficacy of co-processing within the VAB and decide what mitigations may be appropriate.