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At least 667 records · Page 37

NASA GeneLab RNASeq Consensus Pipeline: A Nextflow Implementation

The NASA GeneLab project (genelab.nasa.gov) seeks to accelerate space biology research through cataloging and democratizing omics data. Since raw omics data is largely inaccessible to non-bioinformaticians, GeneLab works with the scientific community to develop standard processing pipelines to generate and publish processed data. Unlike raw data, processed data has greater immediate value to a wide range of users with varying technical backgrounds and computational capabilities. Standardizing processing workflows is essential to match the pace of raw data generation, ensure reproducibility, and enable standardized processed data for comparison across datasets. Previously, GeneLab developed a standardized pipeline for processing RNAseq data, referred to as the ‘GeneLab RNAseq Consensus Pipeline (RCP)’, in collaboration with GeneLab’s Analysis Working Groups. The work presented here is a Nextflow implementation of GeneLab’s RCP that automates and accelerates data processing of RNASeq datasets hosted on GeneLab. In addition to the core data processing, the workflow also includes staging of GeneLab raw data and a robust verification and validation (V&V) program that runs after each processing step to identify errors in real-time, stop additional downstream computation, and preserve computational resources. The workflow, including the staging and V&V functionality, is open source for others to reuse and modify at https://github.com/nasa/GeneLab_Data_Processing/tree/master/RNAseq.

Jonathan D Oribello↗

Gaussian membership functions are most adequate in representing uncertainty in measurements

In rare situations, like fundamental physics, we perform experiments without knowing what their results will be. In the majority of real-life measurement situations, we more or less know beforehand what kind of results we will get. Of course, this is not the precise knowledge of the type 'the result will be between alpha - beta and alpha + beta,' because in this case, we would not need any measurements at all. This is usually a knowledge that is best represented in uncertain terms, like 'perhaps (or 'most likely', etc.) the measured value x is between alpha - beta and alpha + beta.' Traditional statistical methods neglect this additional knowledge and process only the measurement results. So it is desirable to be able to process this uncertain knowledge as well. A natural way to process it is by using fuzzy logic. But, there is a problem; we can use different membership functions to represent the same uncertain statements, and different functions lead to different results. What membership function do we choose? In the present paper, we show that under some reasonable assumptions, Gaussian functions mu(x) = exp(-beta(x(exp 2))) are the most adequate choice of the membership functions for representing uncertainty in measurements. This representation was efficiently used in testing jet engines to airplanes and spaceships.

Kreinovich, V.↗

An Efficient, Highly Flexible Multi-Channel Digital Downconverter Architecture

In this innovation, a digital downconverter has been created that produces a large (16 or greater) number of output channels of smaller bandwidths. Additionally, this design has the flexibility to tune each channel independently to anywhere in the input bandwidth to cover a wide range of output bandwidths (from 32 MHz down to 1 kHz). Both the flexibility in channel frequency selection and the more than four orders of magnitude range in output bandwidths (decimation rates from 32 to 640,000) presented significant challenges to be solved. The solution involved breaking the digital downconversion process into a two-stage process. The first stage is a 2 oversampled filter bank that divides the whole input bandwidth as a real input signal into seven overlapping, contiguous channels represented with complex samples. Using the symmetry of the sine and cosine functions in a similar way to that of an FFT (fast Fourier transform), this downconversion is very efficient and gives seven channels fixed in frequency. An arbitrary number of smaller bandwidth channels can be formed from second-stage downconverters placed after the first stage of downconversion. Because of the overlapping of the first stage, there is no gap in coverage of the entire input bandwidth. The input to any of the second-stage downconverting channels has a multiplexer that chooses one of the seven wideband channels from the first stage. These second-stage downconverters take up fewer resources because they operate at lower bandwidths than doing the entire downconversion process from the input bandwidth for each independent channel. These second-stage downconverters are each independent with fine frequency control tuning, providing extreme flexibility in positioning the center frequency of a downconverted channel. Finally, these second-stage downconverters have flexible decimation factors over four orders of magnitude The algorithm was developed to run in an FPGA (field programmable gate array) at input data sampling rates of up to 1,280 MHz. The current implementation takes a 1,280-MHz real input, and first breaks it up into seven 160-MHz complex channels, each spaced 80 MHz apart. The eighth channel at baseband was not required for this implementation, and led to more optimization. Afterwards, 16 second stage narrow band channels with independently tunable center frequencies and bandwidth settings are implemented A future implementation in a larger Xilinx FPGA will hold up to 32 independent second-stage channels.

Goodhart, Charles E.↗

A real time data acquisition system by satellite relay

The overall aim was to evaluate the future usefulness of satellites in the performance of coordination and management functions related to the operation of flood control and other multipurpose projects in New England. Results on the data collection portion of the work are presented. The principal task was to develop statistics that demonstrate the relationship between conventional means of acquiring hydrologic data and the contribution made by using the satellite and its data collection platforms. Main interest was in determining the availability, reliability and usability of the data. Significant results on DCS show that the DCP's are reliable and useful and satellite data collection appears feasible on a nationwide basis.

Cooper, S.↗

HEO multimission navigation concept

As part of the Network Consolidation Program, the 26-meter Tracking and Communication Subnet was transferred to JPL. Along with this transfer JPL assumed responsibility for tracking and navigation support for Earth orbiter missions normally tracked by the 26-meter sites. The High Earth Orbiter (HEO) Multimission Navigation Facility was formed as a component of the Deep Space Network (DSN) Tracking System for the purpose of supporting Earth orbiter missions and certain classes of deep space missions. This facility has been implemented on a dedicated VAX 11/780 minicomputer within the Network Operations Control Center (NOCC). The primary function of the system is to process radio metric data and estimate the orbit of a spacecraft in near-Earth or deep space environment. The system is capable of processing radio metric data in near-real time and providing the quick turnaround required for Earth orbiter operations. It is also capable of generating precision spacecraft ephemeris for use by the NOCC Support Subsystem and external agencies. This article discusses the implementation and functional operation of the Multimission Navigation Subsystem and describes the support that has been provided for an array of missions.

Ellis, J.↗

Report on Alternative Devices to Pyrotechnics on Spacecraft

Pyrotechnics accomplish many functions on today's spacecraft, possessing minimum volume/weight, providing instantaneous operation on demand, and requiring little input energy. However, functional shock, safety, and overall system cost issues, combined with emergence and availability of new technologies question their continued use on space missions. Upon request from the National Aeronautics and Space Administration's (NASA) Program Management Council (PMC), Langley Research Center (LaRC) conducted a survey to identify and evaluate state-of-the-art non-explosively actuated (NEA) alternatives to pyrotechnics, identify NEA devices planned for NASA use, and investigate potential interagency cooperative efforts. In this study, over 135 organizations were contacted, including NASA field centers, Department of Defense (DOD) and other government laboratories, universities, and American and European industrial sources resulting in further detailed discussions with over half, and 18 face-to-face briefings. Unlike their single use pyrotechnic predecessors, NEA mechanisms are typically reusable or refurbishable, allowing flight of actual tested units. NEAs surveyed include spool-based devices, thermal knife, Fast Acting Shockless Separation Nut (FASSN), paraffin actuators, and shape memory alloy (SMA) devices (e.g., Frangibolt). The electro-mechanical spool, paraffin actuator and thermal knife are mature, flight proven technologies, while SMA devices have a limited flight history. There is a relationship between shock, input energy requirements, and mechanism functioning rate. Some devices (e.g., Frangibolt and spool based mechanisms) produce significant levels of functional shock. Paraffin, thermal knife, and SMA devices can provide gentle, shock-free release but cannot perform critically timed, simultaneous functions. The FASSN flywheel-nut release device possesses significant potential for reducing functional shock while activating nearly instantaneously. Specific study recommendations include: (1) development of NEA standards, specifically in areas of material characterization, functioning rates, and test methods; (2) a systems level approach to assure successful NEA technology application; and (3) further investigations into user needs, along with industry/government system-level real spacecraft cost benefit trade studies to determine NEA application foci and performance requirements. Additional survey observations reveal an industry and government desire to establish partnerships to investigate remaining unknowns and formulate NEA standards, specifically those driven by SMAs. Finally, there is increased interest and need to investigate alternative devices for such functions as stage/shroud separation and high pressure valving. This paper summarizes results of the NASA-LaRC survey of pyrotechnic alternatives. State of-the-art devices with their associated weight and cost savings are presented. Additionally, a comparison of functional shock characteristics of several devices are shown, and potentially related technology developments are highlighted.

Lucy, M. H.↗

A Multi-Function Guidance, Navigation and Control System for Future Earth and Space Missions

Over the past several years the Guidance, Navigation and Control Center (GNCC) at NASA's Goddard Space Flight Center (GSFC) has actively engaged in the development of advanced GN&C technology to enable future Earth and Space science missions. The Multi-Function GN&C System (MFGS) design presented in this paper represents the successful coalescence of several discrete GNCC hardware and software technology innovations into one single highly integrated, compact, low power and low cost unit that simultaneously provides autonomous real time on-board attitude determination solutions and navigation solutions with accuracies that satisfy many future GSFC mission requirements. The MFGS is intended to operate as a single self-contained multifunction unit combining the functions now typically performed by a number of hardware units on a spacecraft. However, recognizing the need to satisfy a variety of future mission requirements, design provisions have been included to permit the unit to interface with a number of external remotely mounted sensors and actuators such as magnetometers, sun sensors, star cameras, reaction wheels and thrusters. The result is a highly versatile MFGS that can be configured in multiple ways to suit a realm of mission-specific GN&C requirements. It is envisioned that the MFGS will perform a mission enabling role by filling the microsat GN&C technology gap. In addition, GSFC believes that the MFGS could be employed to significantly reduce volume, power and mass requirements on conventional satellites.

Gambino, Joel↗

Atmosphere Explorer control system software (version 2.0)

The Atmosphere Explorer Control System (AECS) was developed to provide automatic computer control of the Atmosphere Explorer spacecraft and experiments. The software performs several vital functions, such as issuing commands to the spacecraft and experiments, receiving and processing telemetry data, and allowing for extensive data processing by experiment analysis programs. The AECS was written for a 48K XEROX Data System Sigma 5 computer, and coexists in core with the XDS Real-time Batch Monitor (RBM) executive system. RBM is a flexible operating system designed for a real-time foreground/background environment, and hence is ideally suited for this application. Existing capabilities of RBM have been used as much as possible by AECS to minimize programming redundancy. The most important functions of the AECS are to send commands to the spacecraft and experiments, and to receive, process, and display telemetry data.

Mocarsky, W.↗

X-29A Lateral-Directional Stability and Control Derivatives Extracted From High-Angle-of-Attack Flight Data

The lateral-directional stability and control derivatives of the X-29A number 2 are extracted from flight data over an angle-of-attack range of 4 degrees to 53 degrees using a parameter identification algorithm. The algorithm uses the linearized aircraft equations of motion and a maximum likelihood estimator in the presence of state and measurement noise. State noise is used to model the uncommanded forcing function caused by unsteady aerodynamics over the aircraft at angles of attack above 15 degrees. The results supported the flight-envelope-expansion phase of the X-29A number 2 by helping to update the aerodynamic mathematical model, to improve the real-time simulator, and to revise flight control system laws. Effects of the aircraft high gain flight control system on maneuver quality and the estimated derivatives are also discussed. The derivatives are plotted as functions of angle of attack and compared with the predicted aerodynamic database. Agreement between predicted and flight values is quite good for some derivatives such as the lateral force due to sideslip, the lateral force due to rudder deflection, and the rolling moment due to roll rate. The results also show significant differences in several important derivatives such as the rolling moment due to sideslip, the yawing moment due to sideslip, the yawing moment due to aileron deflection, and the yawing moment due to rudder deflection.

Iliff, Kenneth W.↗

A numerical solution of the Navier-Stokes equations for supercritical fluid thermodynamic analysis

An explicit numerical solution of the compressible Navier-Stokes equations is applied to the thermodynamic analysis of supercritical oxygen in the Apollo cryogenic storage system. The wave character is retained in the conservation equations which are written in the basic fluid variables for a two-dimensional Cartesian coordinate system. Control-volume cells are employed to simplify imposition of boundary conditions and to ensure strict observance of local and global conservation principles. Non-linear real-gas thermodynamic properties responsible for the pressure collapse phenomonon in supercritical fluids are represented by tabular and empirical functions relating pressure and temperature to density and internal energy. Wall boundary conditions are adjusted at one cell face to emit a prescribed mass flowrate. Scaling principles are invoked to achieve acceptable computer execution times for very low Mach number convection problems. Detailed simulations of thermal stratification and fluid mixing occurring under low acceleration in the Apollo 12 supercritical oxygen tank are presented which model the pressure decay associated with de-stratification induced by an ordinary vehicle maneuver and heater cycle operation.

Heinmiller, P. J.↗

Computer programs for calculation of matrix stability and frequency response from a state-space system description

FORTRAN computer subroutines stemming from requirements to process state variable system equations for systems of high order are presented. They find the characteristic equation of a matrix using the method of Danilevsky, the number of roots with positive real parts using the Routh-Horwitz alternate formulation, convert a state variable system description to a Laplace transfer function using the method of Bollinger, and evaluate that transfer function and obtain its frequency response. A sample problem is presented to demonstrate use of the subroutines.

Seidel, R. C.↗

Numerical precision and data structures

Technical proposals and recommendations for revising FORTRAN were studied and categorized. In the area of numerical precision, the proposals basically agree on a set of necessary parameters, although a wide range of nomenclature and specific function names are used. Environmental parameters identified include the following: (1) base of floating point representation, (2) largest positive real number, exponent and integer, (3) largest negative real number, exponent and integer, (4) number of significant digits, and (5) exponent bias.

Schenk, W.↗

A study of a space communication system for the control and monitoring of the electric distribution system. Volume 2: Supporting data and analyses

It is technically feasible to design a satellite communication system to serve the United States electric utility industry's needs relative to load management, real-time operations management, remote meter reading and to determine the costs of various elements of the system. The functions associated with distribution automation and control and communication system requirements are defined. Factors related to formulating viable communication concepts, the relationship of various design factors to utility operating practices, and the results of the cost analysis are discussed The system concept and several ways in which the concept could be integrated into the utility industry are described.

Vaisnys, A.↗

Oceanic Satellite Data Distribution System

The Satellite Data Distribution System (SDDS) serves to process satellite-derived ocean observations, generate ocean analysis and forecast products, and distribute the products to a limited set of commercial users. The SDDS functions in series with the U.S. Navy Fleet Numerical Oceanography Center (FNOC) to provide products on a near-real-time basis to commercial marine industries. Conventional meteorological and oceanographic observations provided to FNOC serve as the input set to the numerical analysis and forecast models. Large main-frame computers are used to analyze and forecast products on a routine, operational basis (at 6-hour and 12-hour synoptic times). The products, reformatted to meet commercial users needs, are transferred to a NASA-owned computer for storage and distribution. Access to the information is possible either by a commercial dial-up packet-switching network or by a direct computer-computer connection.

Montgomery, D. R.↗

Propagation of sound waves in tubes of noncircular cross section

Plane-acoustic-wave propagation in small tubes with a cross section in the shape of a flattened oval is described. Theoretical descriptions of a plane wave propagating in a tube with circular cross section and between a pair of infinite parallel plates, including viscous and thermal damping, are expressed in similar form. For a wide range of useful duct sizes, the propagation constant (whose real and imaginary parts are the amplitude attenuation rate and the wave number, respectively) is very nearly the same function of frequency for both cases if the radius of the circular tube is the same as the distance between the parallel plates. This suggests that either a circular-cross-section model or a flat-plate model can be used to calculate wave propagation in flat-oval tubing, or any other shape tubing, if its size is expressed in terms of an equivalent radius, given by g = 2 x (cross-sectional area)/(length of perimeter). Measurements of the frequency response of two sections of flat-oval tubing agree with calculations based on this idea. Flat-plate formulas are derived, the use of transmission-line matrices for calculations of plane waves in compound systems of ducts is described, and examples of computer programs written to carry out the calculations are shown.

Richards, W. B.↗

Facing page test for the astronaut science advisor presentation

The goal of the Astronaut Science Advisor (ASA) project is to improve the scientific return of experiments performed in space by providing astronaut experimenters with an 'intelligent assistant' that encapsulates much of the domain- and experiment-related knowledge commanded by the Principal Investigator (PI) on the ground. By using expert systems technology and the availability of flight-qualified personal computers, it is possible to encode the requisite knowledge and make it available to astronauts as they perform experiments in space. The system performs four major functions: diagnosis and troubleshooting of experiment apparatus, data collection, protocol management, and detection of interesting data. The experiment used for development of the system measures human adaptation to weightlessness in the context of the neurovestibular system. This so-called 'Rotating Dome' experiment was flown on the recent Spacelab Life Sciences One (SLS-1) Mission. This mission was used as an opportunity to test some of the system's functionality. Experiment data was downlinked from the orbiter, and the system then captured the data and analyzed it in real time. The system kept track of the time being used by the experiment, recognized occurrences of interesting data, summarized data statistically and generated potential new protocols that could be used to optimize the course of the experiment.

Compton, Michael M.↗

Atmospheric Nitrogen Fluorescence Yield

Several existing and planned experiments estimate the energies of ultra-high energy cosmic rays from air showers using the atmospheric nitrogen fluorescence. The nitrogen fluorescence yield from air shower electrons depends on the atmospheric composition. We will discuss the uncertainties in the fluorescence yield form electrons in the real atmosphere and describe a concept for a small balloon payload to measure the atmospheric fluorescence yield as a function of attitude.

Adams, J. H., Jr.↗

Scalability Analysis and Use of Compression at the Goddard DAAC and End-to-End MODIS Transfers

The goal of this task is to analyze the performance of single and multiple FTP transfer between SCF's and the Goddard DAAC. We developed an analytic model to compute the performance of FTP sessions as a function of various key parameters, implemented the model as a program called FTP Analyzer, and carried out validations with real data obtained by running single and multiple FTP transfer between GSFC and the Miami SCF. The input parameters to the model include the mix to FTP sessions (scenario), and for each FTP session, the file size. The network parameters include the round trip time, packet loss rate, the limiting bandwidth of the network connecting the SCF to a DAAC, TCP's basic timeout, TCP's Maximum Segment Size, and TCP's Maximum Receiver's Window Size. The modeling approach used consisted of modeling TCP's overall throughput, computing TCP's delay per FTP transfer, and then solving a queuing network model that includes the FTP clients and servers.

Menasce, Daniel A.↗