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At least 667 records · Page 37

The effect of growth phase and medium on the use of the firefly adenosine triphosphate (ATP) assay for the quantitation of bacteria

Luciferase assay for adenosine triphosphate (ATP) was used as a rapid method to determine the number of bacteria in a urine sample after nonbacterial components were removed. Accurate cellular ATP values, determined when bacteria were grown in an environment similar to that in which they were found, were necessary for the calculation of bacterial titer in urine. Cellular ATP values vary depending on the extraction method, the cell growth phase, and cell growth conditions. ATP per cell values of stationary E. coli grown in urine were two times greater than ATP per cell values of cells grown in trypticase soy broth. Glucose and urea were examined as possible components responsible for the cellular ATP variation.

Bush, V. N.↗

Quantitative determination of stratospheric aerosol characteristics

The author has identified the following significant results. In the S192 data, a peak was apparent in the lower altitudes that was not present in the shorter wavelengths and grew with increasing wavelength beginning with band 7. For ten S192 wavelengths, the relative altitude increment was determined by knowledge of the relative position of the highest point in the scan arc. Using this scheme, results of scaling and inverting data for passes 47 and 61 were put into two models. Each result had three chart representations: (1) limb brightness measurement, (2) attenuation coefficients, and (3) ratio of the aerosol and Rayleigh coefficients to accentuate layers.

Tingey, D. L.↗

Quantitative observation of light flash sensations experiment MA-106

Light flashes caused by the interaction of cosmic particles with the visual apparatus have been observed by astronauts on all space missions since Apollo 11. This Apollo Soyuz Test Project experiment compared measurements of the observer's visual sensitivity with measurements of the ambient radiation environment and with the frequency and character of the flashes observed. The data obtained reveal a latitude dependence of the frequency of observed flashes. This distribution of flashes is correlated with the distribution of cosmic particles with stopping power greater than 15 keV/ micrometers in the eye. The interaction of dark adaptation, specific ionization, and range of particles in the retina as factors in the visualization of particle passage is discussed.

Budinger, T. F.↗

Use of LARS system for the quantitative determination of smoke plume lateral diffusion coefficients from ERTS images of Virginia

A technique for measuring smoke plume of large industrial sources observed by satellite using LARSYS is proposed. A Gaussian plume model is described, integrated in the vertical, and inverted to yield a form for the lateral diffusion coefficient, Ky. Given u, wind speed; y sub l, the horizontal distance of a line of constant brightness from the plume symmetry axis a distance x sub l, downstream from reference point at x=x sub 2, y=0, then K sub y = u ((y sub 1) to the 2nd power)/2 x sub 1 1n (x sub 2/x sub 1). The technique is applied to a plume from a power plant at Chester, Virginia, imaged August 31, 1973 by LANDSAT I. The plume bends slightly to the left 4.3 km from the source and estimates yield Ky of 28 sq m/sec near the source, and 19 sq m/sec beyond the bend. Maximum ground concentrations are estimated between 32 and 64 ug/cu m. Existing meteorological data would not explain such concentrations.

Blais, R. N.↗

Quantitative sediment mapping from remotely sensed multispectral data

Multiple regression techniques were used to correlate ERTS-1 data with measured values of suspended sediment. The resultant equation was applied to a different ERTS scene using a deepwater area for atmospheric calibration. This approach provides greater accuracy than previously reported methods. Suspended sediment contours are plotted for the Potomac River, Virginia, using Chesapeake Bay deep water for atmospheric calibration.

Johnson, R. W.↗

Acquisition of quantitative physiological data and computerized image reconstruction using a single scan TV system

A single-scan radiography system has been interfaced to a minicomputer, and the combined system has been used with a variety of fluoroscopic systems and image intensifiers available in clinical facilities. The system's response range is analyzed, and several applications are described. These include determination of the gray scale for typical X-ray-fluoroscopic-television chains, measurement of gallstone volume in patients, localization of markers or other small anatomical features, determinations of organ areas and volumes, computer reconstruction of tomographic sections of organs in motion, and computer reconstruction of transverse axial body sections from fluoroscopic images. It is concluded that this type of system combined with a minimum of statistical processing shows excellent capabilities for delineating small changes in differential X-ray attenuation.

Baily, N. A.↗

Quantitative measurement of the "feel" of fabric

Device handle of fabric is a property related to flexibility, pliability, or compressibility. Technique requires extraction of flat circular sample of fabric through convergent nozzle.

Alley, V. L., Jr.↗

Quantitative three-dimensional dynamic imaging of structure and function of the cardiopulmonary and circulatory systems in all regions of the body

The background for, and design of a third generation, general purpose, all electronic spatial scanning system, the DSR is described. Its specified performance capabilities provide dynamic and stop action three dimensional spatial reconstructions of any portion of the body based on a minimum exposure time of 0.01 second for each 28 multiplanar 180 deg scanning set, a maximum scan repetition rate of sixty 28 multiplane scan sets per second, each scan set consisting of a maximum of 240 parallel cross sections of a minimum thickness of 0.9 mm, and encompassing a maximum cylindrical volume about 23 cm in length and up to 38 cm in diameter.

Sturm, R. E.↗

Acquisition of quantitative physiological data and computerized image reconstruction using a single scan TV system

Single scan operation of television X-ray fluoroscopic systems allow both analog and digital reconstruction of tomographic sections from single plan images. This type of system combined with a minimum of statistical processing showed excellent capabilities for delineating small changes in differential X-ray attenuation. Patient dose reduction is significant when compared to normal operation or film recording. Flat screen, low light level systems were both rugged and light in weight, making them applicable for a variety of special purposes. Three dimensional information was available from the tomographic methods and the recorded data was sufficient when used with appropriate computer display devices to give representative 3D images.

Baily, N. A.↗

Quantitative determination of zero-gravity effects on electronic materials processing germanium crystal growth with simultaneous interface demarcation. Experiment MA-060

Experiment MA-060 was designed to establish the crystal growth and segregation characteristics of a melt in a directional solidification configuration under near zero-g conditions. The interface demarcation technique was incorporated into the experiment since it constitutes a unique tool for recording the morphology of the growth rate throughout solidification, and for establishing an absolute time reference framework for all stages of the solidification process. An extensive study was performed of the germanium crystals grown during the Apollo-Soyuz Test Project mission. It was found that single crystal growth was achieved and that the interface demarcation functioned successfully. There was no indication that convection driven by thermal or surface tension gradients was present in the melt. The gallium segregation, in the absence of gravity, was found to be fundamentally different in its initial and its subsequent stages from that of the ground-based tests. None of the existing theoretical models for growth and segregation can account for the observed segregation behavior in the absence of gravity.

Gatos, H. C.↗

Quantitative comparisons of type III radio burst intensity and fast electron flux at 1 AU

We compare the flux of fast solar electrons and the intensity of the type III radio emission generated by these particles at 1 AU. We find that there are two regimes in the generation of type III radiation: one where the radio intensity is linearly proportional to the electron flux, and the second regime, which occurs above a threshold electron flux, where the radio intensity is proportional to the approximately 2.4 power of the electron flux. This threshold appears to reflect a transition to a different emission mechanism.

Fitzenreiter, R. J.↗

The quantitative properties of three soft X-ray flare kernels observed with the AS&E X-ray telescope on Skylab

The physical parameters for the kernels of three solar X-ray flare events have been deduced using photographic data from the S-054 X-ray telescope on Skylab as the primary data source and 1-8 and 8-20 A fluxes from Solrad 9 as the secondary data source. The kernels had diameters of about 5-7 seconds of arc and in two cases electron densities at least as high as 0.3 trillion per cu cm. The lifetimes of the kernels were 5-10 min. The presence of thermal conduction during the decay phases is used to argue: (1) that kernels are entire, not small portions of, coronal loop structures, and (2) that flare heating must continue during the decay phase. We suggest a simple geometric model to explain the role of kernels in flares in which kernels are identified with emerging flux regions.

Kahler, S. W.↗

Quantitative mass distribution models for Mare Orientale

Six theoretical models for the mass distribution of Mare Orientale were tested using five gravity profiles extracted from radio-tracking data of orbiting spacecraft. The models with surface mass and moho relief produced the best results. Although there is a mascon-type anomaly in the central maria region, Mare Orientale is a large negative gravity anomaly. This is produced primarily by the empty ring basin. Had the basin filled with maria material it seems likely that it would have produced a mascon such as those presently existing in flooded frontside circular basins.

Sjogren, W. L.↗

A satellite technique for quantitatively mapping rainfall rates over the oceans

A theoretical model for calculating microwave radiative transfer in raining atmospheres is developed. These calculations are compared with microwave brightness temperatures at a wavelength of 1.55 cm measured by the Electrically Scanning Microwave Radiometer (ESMR) on the Nimbus-5 satellite and rain rates derived from WSR-57 meteorological radar measurements. A specially designed ground-based verification experiment was also performed, wherein upward-viewing microwave brightness temperature measurements at wavelengths of 1.55 and 0.81 cm were compared with directly measured rain rates. It is shown that, over ocean areas, brightness temperature measurements from ESMR may be interpreted in terms of rain rate with about an accuracy of a factor of 2 over the range 1 to 25 mm/hr rain rate.

Wilheit, T. T.↗