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70 records · Page 4

Energy efficiency and thermal resilience analysis for row houses using representative building energy models

Row houses, a prevalent medium-density urban archetype, were popularized during industrial era to accommodate the working class in the U.S. cities. Today, many of these structures are deteriorating, exacerbating the energy cost for residents with limited economic resources. Moreover, many households that lack Air Conditioning (AC) are vulnerable to extreme summer heat, an issue expected to worsen in the future. Energy-efficient retrofits can help mitigate these challenges. However, current literature provides limited insight into how existing building energy codes impact the energy efficiency and thermal resilience of row houses in current and future weather scenarios. This study provides a detailed method to address this gap by employing calibrated representative energy models of row houses as a baseline, and evaluating the annual energy savings and summertime thermal safety improvements from code compliant retrofits under current and future typical weather conditions. Using Baltimore City as a case study, results show that under current weather, retrofitting row houses yields significant energy savings along with notable thermal resilience improvements. The end unit row house realizes higher energy savings, whereas the interior unit experiences greater thermal resilience improvement after retrofitting. In future, retrofitted row houses can still save energy, but residents will face numerous hours of fatal indoor temperatures without AC in summer. Additionally, findings reveal stark zone-wise variations in thermal safety, and elevated humidity risks in certain zones post-retrofit, emphasizing the need for zonal retrofit strategies and better natural ventilation strategies.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

Predicting weather impacts on corn production in a data-limited region using a transfer learning approach

The stability of food supply and prices may depend more on annual changes in yields from year-to-year variability in weather than on longer-term average changes from changing climatic conditions. However, the absence of high-quality data on crop yields at fine spatial resolutions in many regions of the world makes it challenging to statistically model their response to interannual variability in weather patterns. Therefore, there is a need for empirical methods that can project annual crop yield changes even in limited data regions. Here, we propose a transfer learning algorithm that uses high spatial resolution data from one region to project yields in another region with more limited data. The goal of our work is to understand what data types can be beneficial for transferring learning from a source region to a very different target region with more limited data. We utilize Long Short-Term Memory to develop a transfer learning model that is trained on historical county-level corn yield in the United States and predicts district-level corn yield variations in India. Even using smaller amounts of data in India, simulating a data-scarce region, we achieve an average root mean square error of 0.48 bu acre−1 in predicting interannual yield variations. Using Shapley values to interpret results, we explore the contribution of the different weather parameters to interannual yield variability and find a larger influence of precipitation-related variables. Our study demonstrates the usefulness of this method for transferring models of weather impacts on crop yields trained on a data-rich country to one with more limited data. It suggests the potential of applying the transfer learning model to mitigate the need for extensive raw data globally.

Vishwakarma, Srishti [ORNL] (ORCID:000000031674419↗

Comparison of greenhouse gas emission estimates from six hydropower reservoirs using modeling versus field surveys

As with most aquatic ecosystems, reservoirs play an important role in the global carbon (C) cycle and emit greenhouse gases (GHG) as carbon dioxide (CO 2 ) and methane (CH 4 ). However, GHG emissions from reservoirs are poorly quantified, especially in temperate systems, resulting in high uncertainty. We compared reservoir C emission estimates and uncertainty of diffusive, ebullitive, and degassing pathways in six hydropower reservoirs in the southeastern United States among four data sources: two field-based surveys and two models (including the GHG Reservoir “G-res” Tool). We found that CH 4 diffusion was most similar across data sources (modeled minus observed, bias = - 21 g CO 2-eq m -2 y -1 ) and had low relative uncertainty (coefficient of variation, CV = 0.98). On the other hand, CO 2 diffusion was least consistent across data sources (bias = - 518 g CO 2-eq m -2 y -1 ). Both field surveys indicated strong negative CO 2 diffusion (i.e., CO 2 uptake) at all reservoirs, while G-res estimated positive CO 2 diffusion. By extension, total C emissions showed similar discrepancies, leading to high uncertainty in upscaling and interpreting reservoir source-sink dynamics. Finally, CH 4 ebullition had the highest relative uncertainty (CV = 2.77) due to high variability across sites. We discuss limitations of field surveys and these models, including temperature-based annualization methods, varying definitions of ebullition zones, low sampling resolution, and lack of dynamism. Future field efforts focused on capturing variability in CO 2 diffusion and CH 4 ebullition will be especially valuable in reducing uncertainty and improving models to advance our understanding reservoir GHG emissions.

54 ENVIRONMENTAL SCIENCES↗

Annual Performance Testing of Tracer Gas Detectors for use in Radionuclide NESHAP Compliance Testing

Los Alamos National Laboratory’s (LANL’s) compliance with Radionuclide NESHAP0F 1 regulations is managed by the Radioactive Air Emissions Management (RAEM) team, part of LANL’s Compliance Programs group (EPC-CP). One area of the Radionuclide NESHAP addresses requirements for siting a stack sample system. Prior to commissioning a new stack sampling system, the ANSI Standard for stack sampling requires that the stack sample location must meet several criteria, including uniform mixing of tracer gas (sulfur hexafluoride, SF 6 ) and tracer aerosol (liquid oil droplets) in the air stream. This memorandum will specifically focus on the tracer gas portion of the ANSI Standard. For these mix tests, tracer gas is injected into the stack air stream and the resulting air concentrations are measured across the plane of the stack at the proposed sampling location. The coefficient of variation of the media concentration must be under 20% when evaluated over the central 2/3 area of the stack or duct.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Tracking the Sun: Pricing and Design Trends for Distributed Photovoltaic Systems in the United States, 2024 Edition [Slides]

Berkeley Lab’s annual Tracking the Sun report describes trends among grid-connected, distributed solar photovoltaic (PV) and paired PV+storage systems in the United States. For the purpose of this report, distributed solar includes residential systems, roof-mounted non-residential systems, and ground-mounted systems up to 5 MW-AC. Ground-mounted systems larger than 5 MW-AC are covered in Berkeley Lab’s companion annual report, Utility-Scale Solar. The latest edition of the report is based on 3.7 million systems installed through year-end 2023, representing close to 80% of systems installed to date. The report describes and discusses key trends related to: -Project characteristics, including system size, module efficiencies, roof-coverage ratios, prevalence of paired PV with storage, use of module-level power electronics, third-party ownership, mounting configurations, panel orientation, and customer segmentation -Median installed-price trends, both nationally and by state -Variability in pricing according to system size, state, installer, equipment type, and other factors, relying on both descriptive analysis and a multi-variate regression to estimate the effects of key pricing drivers for residential systems installed in 2023.

14 SOLAR ENERGY↗

California Price Response Potential Study

California's energy landscape is undergoing a significant transformation, driven by the increasing integration of renewable energy sources, the increased adoption of distributed energy resources, the electrification of end-use loads, and the growing need for grid efficiency. To address these challenges, recent revisions to the State’s Load Management Standards (LMS) require all of California’s large utilities and community choice aggregators (CCAs) to offer dynamic electricity pricing options to customers by 2027. Dynamic pricing, which involves varying electricity rates based on real-time supply and demand conditions, offers a promising solution for optimizing grid operations, reducing costs, and incentivizing efficient use of grid capacity. Effective implementation of dynamic pricing requires understanding the potential impacts on customer bills, system load, and the cost-effectiveness of automation technologies. This study aims to evaluate the load response of various end-use devices to hourly dynamic prices. The end-uses studied here are space cooling, space heating, water heating, crop irrigation, pool and spa pumps, and electric vehicle (EV) charging, all for both residential and commercial applications, except for crop irrigation. In 2030, these end uses are forecasted to account for 18% of annual electricity demand in the state, but 40% of demand in the peak net load hour. By modeling possible price-responsive load dispatch algorithms and assessing the resulting impacts on both individual bills and the overall grid, we seek to inform policymakers and utilities about the potential benefits and challenges associated with dynamic pricing, and considerations for the design of dynamic pricing tariffs. Additionally, we will explore the cost effectiveness of adopting automation technologies to enable devices to respond more effectively to real-time price signals. This study considers a range of price profiles, accounting for differences across utilities and customer classes, and presents scenarios for dynamic price design via variation in the percentage of total customer electric costs that are allocated dynamically (versus constituting a fixed portion of the hourly volumetric price). We present results focused primarily on 2030, forecasting electricity prices under both low and high-cost scenarios, to inform longer-term tariff design considerations. We design tariffs by starting with 2019 prices that were calculated according to CalFUSE guidance (CPUC, 2022) and that have been used in recent studies; these prices are all-in volumetric rates that vary by utility and are revenue-neutral to each customer class. They are developed by considering six electricity cost components that are allocated hourly based on system load indicators (gross and net load, and wholesale prices). These prices are forecasted to 2030 for low and high cost scenarios, considering recent trends in total electricity costs with and without years of substantial wildfire mitigation investments. These tariffs, which allocate all costs on an hourly basis, are considered our “Full” dynamic tariff design scenario, while two additional scenarios explore allocating a portion of costs as a flat volumetric charge: the “Medium” scenario allocates 50% of revenue dynamically (and keeps 50% flat), while the “Mild” scenario allocates 20% of revenue dynamically. The 20% dynamic allocation on the Mild scenario aims to represent a case where only the marginal operating costs of the grid are included in the dynamic price.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

Future marine biofuels in the port of Seattle region

Marine transportation, a vital global sector, emits 3% of global annual greenhouse gas emissions, which are predicted to increase in the future. Marine biofuels derived from biomass or waste sources like wood residue, waste oil and municipal solid waste can be used for decarbonization. However, limited studies have explored if sufficient marine biofuels could be produced and supplied to major regional ports given feedstock, supply chain and technological constraints. We fill this gap by evaluating the feasibility of supplying marine biofuels to the Port of Seattle. The Regional Bio-Economy Model (RBEM) and the Freight and Fuel Transportation Optimization Tool (FTOT) are used to build scenarios for simulating marine biofuel production in the Port region. We harmonized technoeconomic assumptions for RBEM and FTOT, input FTOT feedstock utilization and routing outputs into RBEM, and modelled conversion, feedstock, and policy scenario variations in RBEM. In RBEM, overall biofuel production was constrained primarily by the biofuel cost, and then by feedstock availability. Providing policy incentives and reducing permitting time frames alleviated these constraints and spurred the buildout of a robust industry through industrial learning dynamics in the initial years. With these measures in place, the RBEM results show that 100% of fuel demand at the Port can be supplied by biofuels with policy incentives and suitable technoeconomic conditions, but the addition of transportation cost considerations using FTOT led to 27.8% of demand being able to be met by biofuels at reasonable fuel delivery cost.

09 BIOMASS FUELS↗

Ventenata dubia projected to expand in the western United States despite future novel conditions

Abstract Distributions of both native and invasive species are expected to shift under future climate. Species distribution models (SDMs) are often used to explore future habitats, but sources of uncertainty including novel climate conditions may reduce the reliability of future projections. We explore the potential spread of the invasive annual grass ventenata ( Ventenata dubia ) in the western United States under both current and future climate scenarios using boosted regression tree models and 30 global climate models (GCMs). We quantify novel climate conditions, prediction variability arising from both the SDMs and GCMs, and the agreement among GCMs. Results demonstrate that currently suitable habitat is concentrated inside the invaded range of the northwest, but substantial habitat exists outside the invaded range in the Southern Rockies and southwestern US mountains. Future suitability projections vary greatly among GCMs, but GCMs commonly projected decreased suitability in the invaded range and increased suitability along higher elevations of interior mountainous areas. Climate novelty did not appear to undermine the prediction reliability in many cases where the climate–species relationship was fully represented by the occurrence data. GCM‐derived variability resulting from variation in future cool season precipitation and temperature seasonality was greatest in the Rocky Mountains. SDM‐derived variability was higher in currently suitable habitat, and few GCMs projections agreed that these areas would contain future suitable habitat. However, while prediction variability was high, many GCM projections agreed that parts of the Rocky, Wasatch, and Uinta Mountains would contain highly suitable habitat in the future. As disturbances in the interior mountains occur in coming decades, reducing some natural barriers to invasion, land managers, and conservationists will need to monitor for ventenata in post‐disturbance environments. Changes to invasion potential may not play out for several decades, but results related to current potential may have applications for early detection and rapid response planning.

Environmental Sciences & Ecology↗

Controls on Stable Methane Isotope Values in Northern Peatlands and Potential Shifts in Values Under Permafrost Thaw Scenarios

Abstract Northern peatlands are a globally significant source of methane (CH 4 ), and emissions are projected to increase due to warming and permafrost loss. Understanding the microbial mechanisms behind patterns in CH 4 production in peatlands will be key to predicting annual emissions changes, with stable carbon isotopes (δ 13 C‐CH 4 ) being a powerful tool for characterizing these drivers. Given that δ 13 C‐CH 4 is used in top‐down atmospheric inversion models to partition sources, our ability to model CH 4 production pathways and associated δ 13 C‐CH 4 values is critical. We sought to characterize the role of environmental conditions, including hydrologic and vegetation patterns associated with permafrost thaw, on δ 13 C‐CH 4 values from high‐latitude peatlands. We measured porewater and emitted CH 4 stable isotopes, pH, and vegetation composition from five boreal‐Arctic peatlands. Porewater δ 13 C‐CH 4 was strongly associated with peatland type, with δ 13 C enriched values obtained from more minerotrophic fens (−61.2 ± 9.1‰) compared to permafrost‐free bogs (−74.1 ± 9.4‰) and raised permafrost bogs (−81.6 ± 11.5‰). Variation in porewater δ 13 C‐CH 4 was best explained by sedge cover, CH 4 concentration, and the interactive effect of peatland type and pH (r 2 = 0.50,p < 0.001). Emitted δ 13 C‐CH 4 varied greatly but was positively correlated with porewater δ 13 C‐CH 4 . We calculated a mixed atmospheric δ 13 C‐CH 4 value for northern peatlands of −65.3 ± 7‰ and show that this value is more sensitive to landscape drying than wetting under permafrost thaw scenarios. Our results suggest northern peatland δ 13 C‐CH 4 values are likely to shift in the future which has important implications for source partitioning in atmospheric inversion models.

Environmental Sciences & Ecology↗

A novel approach to increase accuracy in remotely sensed evapotranspiration through basin water balance and flux tower constraints

Remote sensing-derived evapotranspiration (RSET) products capture the spatiotemporal variations of evapotranspiration (ET) from field to basin scales with unprecedented details. However, their accuracy varies across RSET estimation methods and diverse hydroclimate regions. While ET modeling efforts to account for biophysical processes and controlling parameters have made good progress in recent years, a parallel approach of integrating in-situ ET with RSET could reduce biases in RSET products. Basin water balance ET (WBET) and flux tower ET are widely applied to evaluate RSET accuracy, yet such ET measurements are rarely used for RSET bias corrections, especially for large area applications. To address this issue, we propose a novel approach: the water balance equivalence (WABE) method, which generates spatially continuous WBET for correcting biases in RSET products. The WABE method computes synthetic WBET by integrating observed WBET and flux tower-derived FLUXCOM ET, which fills the spatial gaps of observed WBET and generates a spatially continuous WBET dataset. Synthetic WBET (2002–2015 annual average) of eight-digit hydrologic unit code (HUC8) basins across the conterminous United States (CONUS), constituting 44 % (887 out of 2035 basins) of CONUS basins, was determined within 2.0 % (RMSE = 12 %) of observed WBET at CONUS and between 1–12 % (RMSE = 3–33 %) across 18 regions in CONUS. With WABE-based bias corrections, the overall annual bias of RSET decreased from 10 % (RMSE = 34 %) to 6 % (RMSE = 26 %) across 37 flux tower sites. The WABE method offers a new approach for RSET accuracy improvement and shows great promise for large area implementations with a potential to yield substantial benefits for building accurate basin water budgets and water management decisions.

Khand, Kul↗

Intrinsic and environmental drivers of pairwise cohesion in wild Canis social groups

Animals within social groups respond to costs and benefits of sociality by adjusting the proportion of time they spend in close proximity to other individuals in the group (cohesion). Variation in cohesion between individuals, in turn, shapes important group-level processes such as subgroup formation and fission–fusion dynamics. Although critical to animal sociality, a comprehensive understanding of the factors influencing cohesion remains a gap in our knowledge of cooperative behavior in animals. We tracked 574 individuals from six species within the genus Canis in 15 countries on four continents with GPS telemetry to estimate the time that pairs of individuals within social groups spent in close proximity and test hypotheses regarding drivers of cohesion. Pairs of social canids (Canis spp.) varied widely in the proportion of time they spent together (5%–100%) during seasonal monitoring periods relative to both intrinsic characteristics and environmental conditions. The majority of our data came from three species of wolves (gray wolves, eastern wolves, and red wolves) and coyotes. For these species, cohesion within social groups was greatest between breeding pairs and varied seasonally as the nature of cooperative activities changed relative to annual life history patterns. Across species, wolves were more cohesive than coyotes. For wolves, pairs were less cohesive in larger groups, and when suitable, small prey was present reflecting the constraints of food resources and intragroup competition on social associations. Pair cohesion in wolves declined with increased anthropogenic modification of the landscape and greater climatic variability, underscoring challenges for conserving social top predators in a changing world. We show that pairwise cohesion in social groups varies strongly both within and across Canis species, as individuals respond to changing ecological context defined by resources, competition, and anthropogenic disturbance. Our work highlights that cohesion is a highly plastic component of animal sociality that holds significant promise for elucidating ecological and evolutionary mechanisms underlying cooperative behavior.

59 BASIC BIOLOGICAL SCIENCES↗

Data for Attributing Differences of Solar-Induced Chlorophyll Fluorescence (SIF)-Gross Primary Production (GPP) Relationships between Two C4 Crops, Corn and Miscanthus

Information to characterize the solar-induced chlorophyll fluorescence (SIF)-gross primary production (GPP) relationship in C4 cropping systems remains limited. The annual C4 crop corn and perennial C4 crop miscanthus differ in phenology, canopy structure and leaf physiology. Investigating the SIF-GPP relationships in these species could deepen our understanding of SIF-GPP relationships within C4 crops. Using in situ canopy SIF and GPP measurements for both species along with leaf-level measurements, we found considerable differences in the SIF-GPP relationships between corn and miscanthus, with a stronger SIF-GPP relationship and higher slope of SIF-GPP observed in corn compared to miscanthus. These differences were mainly caused by leaf physiology. For miscanthus, high non-photochemical quenching (NPQ) under high light, temperature and water vapor deficit (VPD) conditions caused a large decline of fluorescence yield (ΦF), which further led to a SIF midday depression and weakened the SIF-GPP relationship. The larger slope in corn than miscanthus was mainly due to its higher GPP in mid-summer, largely attributed to the higher leaf photosynthesis and less NPQ. Our results demonstrated variation of the SIF-GPP relationship within C4 crops and highlighted the importance of leaf physiology in determining canopy SIF behaviors and SIF-GPP relationships.

Feedstock Production↗

Fine-scale landscape characteristics, vegetation composition, and snowmelt timing control phenological heterogeneity across low-Arctic tundra landscapes in Western Alaska

The Arctic is warming at over twice the rate of the rest of the Earth, resulting in significant changes in vegetation seasonality that regulates annual carbon, water, and energy fluxes. However, a crucial knowledge gap exists regarding the intricate interplay among climate, permafrost, and vegetation that generates high phenology variability across extensive tundra landscapes. This oversight has led to significant discrepancies in phenological patterns observed across warming experiments, long-term ecological observations, and satellite and modeling studies, undermining our ability to understand and forecast plant responses to climate change in the Arctic. To address this problem, we assessed plant phenology across three low-Arctic tundra landscapes on the Seward Peninsula, Alaska, using a combination of in-situ phenocam observations and high-resolution PlanetScope CubeSat data. We examined the patterns and drivers of phenological diversity across the landscape by (1) quantifying phenological diversity among dominant plant function types (PFTs) and (2) modeling the interrelations between plant phenology and fine-scale landscape features, such as topography, snowmelt, and vegetation. Our findings reveal that both spring and fall phenology varied significantly across Arctic PFTs, accounting for about 25%–44% and 34%–59% of the landscape-scale variation in the start of spring [SOS] and start of fall [SOF], respectively. Deciduous tall shrubs (e.g. alder and willow) had a later SOS (~7 d behind the mean of other PFTs), but completed leaf expansion (within 2 weeks) considerably faster compared to other PFTs. We modeled the landscape-scale variation in SOS and SOF using Random Forest, which showed that plant phenology can be accurately captured by a suite of variables related to vegetation composition, topographic characteristics, and snowmelt timing (variance explained: 53%–68% for SOS and 59%–82% for SOF). Notably, snowmelt timing was a crucial determinant of SOS, a factor often neglected in most spring phenology models. Our study highlights the impact of fine-scale vegetation composition, snow seasonality, and landscape features on tundra phenological heterogeneity. Improved understanding of such considerable intra-site phenological variability and associated proximate controls across extensive Arctic landscapes offers critical insights for representation of tundra phenology in process models and associated impact assessments with climate change.

54 ENVIRONMENTAL SCIENCES↗

A global urban heat island intensity dataset: Generation, comparison, and analysis

The urban heat island (UHI) effect, a phenomenon of local warming over urban areas, is the most well-known impact of urbanization on climate. Globally consistent estimates of the UHI intensity (UHII) are crucial for examining this phenomenon across time and space. However, publicly available UHII datasets are limited and have several constraints: (1) they are for clear-sky surface UHII, not all-sky surface UHII and canopy (air temperature) UHII; (2) the estimation methods often neglect anthropogenic disturbance, introducing uncertainties in the estimated UHII. To address these issues, this study proposes a new dynamic equal-area (DEA) method that can minimize the influence of various confounding factors on UHII estimates through a dynamic cyclic process. Utilizing the DEA method and leveraging various gridded temperature data, we develop a global-scale (>10,000 cities), long-term (over 20 years by month), and multi-faceted (clear-sky surface, all-sky surface, and canopy) UHII dataset. Further, based on these estimates, we provide a comprehensive analysis of the UHII and its trends in global cities. The UHII is found to be greater than zero in >80% of cities, with global annual average magnitudes around 1.0 °C (day) and 0.8 °C (night) for surface UHII, and close to 0.5 °C for canopy UHII. Furthermore, an interannual upward trend in UHII is observed in >60% of cities, with global annual average trends exceeding 0.1 °C/decade (day) and over 0.06 °C/decade (night) for surface UHII, and slightly surpassing 0.03 °C/decade for canopy UHII. Notably, there exists a positive correlation between the magnitude and trend of UHII, suggesting that cities with stronger UHII tend to experience faster growth in UHII. Additionally, discrepancies in UHII are found between different temperature data, stemming not only from distinctions in data types (surface or air temperature) but also from differences in data acquisition times (Terra or Aqua), weather conditions (clear-sky or all-sky), and processing methodologies (with or without gap filling). Overall, our proposed method, dataset, and analysis results have the potential to provide valuable insights for future urban climate studies. The UHII dataset is publicly available at https://doi.org/10.6084/m9.figshare.24821538.

54 ENVIRONMENTAL SCIENCES↗

Microbial community data from throughfall exclusion experiment: Metadata, SI, community composition, LefSe, and FunGuilR data tables from PARCHED Panama tropical forest soils, 2024-2025

Soil contains more carbon (C) than terrestrial vegetation and the atmosphere combined, with some of the largest terrestrial C stocks in tropical rainforests. Soil microbes decompose organic matter, playing a vital role in the storage or loss of soil C. With climate change, drought conditions are predicted to increase in many tropical regions, including both chronic drying and extended drought, potentially influencing these processes. This project explored the effects of chronic and seasonal drying on soil microbial communities across four distinct tropical forests in a long-term drying experiment. We investigated the effects of a chronic drying manipulation on soil microbial community abundance and variation across different forests and seasons. We also compared findings with previously published data from these forests after short-term drying. This project used soils from a long-term drying experiment established in 2018 across four seasonal lowland forests in Panama. Soils were collected from 0 – 10 cm depths during three seasonal periods in control and drying plots in 2024 and 2025 from a total of 32 plots (n = 4 per forest per treatment). The forests varied in baseline rainfall and soil fertility. We calculated alpha and beta diversity indices and compared taxonomic community composition. We found significant biogeographic variation in microbial diversity and taxonomy, with significant differences across the forests and significant effects of the drying treatment. Metadata and sample IDs are within Metadata_16S.csv and Metadata_ITS.csv. Relative abundance tables of every sample at every season are shown in the Excel workbooks 16S Relative Abundance.xlsx and ITS Relative Abundance.xlsx. They are then also shown in CSV files by each taxonomic level. Linear discriminant analysis effect size (LefSe) tables are shown for the full 16S and ITS datasets (n = 96), subsets for every site at every season (n = 8), and then for the forests with each plot merged by season (n = 8). FunGuildR data table of ITS data is uploaded.

Bacteria↗

Root genetics in the field to understand drought adaptation and carbon sequestration (Final Scientific/Technical Report)

For all crop plants, roots play a critical role in growth. Roots anchor the plants, and are the primary site of nutrient and water uptake. Roots are also the main source of C to soil in the form of root tissues and exudates, and thus greatly influence SOM stocks. To perform these functions, primary roots extend into soil, producing a network of branching roots of characteristic form, known as its root system architecture (RSA). RSA varies among species, and among varieties within a species that are adapted to different environments. Root traits are major targets for the second green revolution because of their potential to improve crop productivity, increase drought tolerance and nutrient acquisition, and increase C capture of soil. Improving the quality of roots in maize will be particularly valuable, since this crop is planted on over 92 million acres annually in the US. The future sustainability of agricultural systems relies on their ability to enhance soil organic matter (SOM) storage and reduce GHG emissions, while maintaining or enhancing productivity. This program had two components, Sensors and Models. For the first component, we designed and built a high-throughput phenotyping platform for root pulling of maize plants. This eliminated the physical labor of manually pulling up plants and reduced the number of personnel required down to one. The standardized pulling mechanism allowed recording force curves during the pulling process, providing additional information. We validated that the maximum force for pulling the root system was well-correlated with the root system mass and provided root crowns for further RSA analysis. These root crowns identified significant correlations with 2D root area and root depth, along with 3D root volume, total root length and number of root tips. We then used this system for field-based studies in maize on the genetics of root system architecture and its relation to nitrogen-use efficiency (NUE), including using lines relevant to the Corteva breeding program. Varieties were also evaluated at Corteva sites in the cornbelt and Danforth farm in Missouri, to establish responses across sites. From these studies we have identified genetic loci associated with root traits and created mutant lines for these loci and correlations of root traits with NUE. For the Models component, we worked to incorporate root and soil characteristics into the MEMS 2.0 soil and ecosystem biogeochemical model. Existing soil C models, such as Century, are unable to represent specific root trait interactions with the soil environment and therefore to accurately forecast the potential C sequestration benefits of root breeding under different climatic and soil type conditions. We have developed the MEMS 2.0 ecosystem biogeochemical model to improve quantification of farm-scale soil carbon and greenhouse gas emissions. The new knowledge and large datasets produced by this project will be used to develop and drive an innovative model capable of forecasting the impacts on soil C stocks and nutrient dynamics. An innovation was to use the empirical data from the field studies (in 1, above) to model genetic variation in nitrogen use efficiencies and soil C input. Our work demonstrated that maize root-derived C rapidly replaces existing soil C and after 3 years of continuous maize, up to 20% of soil organic C in the topsoil (0-15cm) and 3% in the subsoil (15-30cm) was contributed by maize. However, this contribution did not entirely represent a net increase. Root C contribution to soil was affected by maize genetics. We have analyzed soils derived from the CSU field trials for C and N stocks, in the different soil physical fractions represented by the MEMS model, using both physical fractionation with elemental analyses, and Fourier transformed infrared spectroscopy. Data will be used to link crop nitrogen use efficiencies with soil C sequestration and provide data to bridge the field trials with the model development, for verification of model predictions. The project had a number of successful outcomes: we have used the new phenotyping platform to identify new genetic loci that can enhance root phenotypes; we have partnered with multiple maize seed companies phenotype varieties in their breeding programs; we have developed the MEMS model that can help inform industry on the potential for carbon sequestration in the agricultural sector, and which is now available at the CSU Soil Carbon Solutions Center for use.

59 BASIC BIOLOGICAL SCIENCES↗