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At least 73 records · Page 4

Multivariate environmental and trait-based controls of transpiration in the Central Amazon Rainforest

Tropical forest tree mortality is increasing due to more severe droughts, yet our understanding of how tree traits and life strategies are linked to drought stress has been limited by measurement scarcity. The BIONTE (BIOmass and NuTrient Experiment) near Manaus, Brazil hosts one of the world’s largest sap flow installations, with sensors in 90 canopy trees across a wood density gradient monitored since June 2022. The 2023 El Niño drought provided a unique opportunity to evaluate how water availability impacts tree transpiration. An interpretable machine learning framework was used to study the complex interactions between transpiration and multiple environmental variables such as soil water availability and vapor pressure deficit (VPD), and how these interactions vary with wood density and individual trees. We found varying responses of transpiration from different trees during the El Niño drought. Transpiration generally increased with temperature, with stronger effects in wetter areas and in trees with low to medium wood density. However, this response was modulated by stomatal sensitivity to VPD, which constrained transpiration under high atmospheric demand, particularly in intermediate-moisture area. The inflection in transpiration rate at high temperatures (>32°C) underscores the role of stomatal and hydraulic regulation in limiting water loss and protecting trees from excessive evaporative demand. Analysis of soil water contribution to transpiration revealed unimodal patterns in wetter area, with peak contributions near 0.45 cm 3 cm -3 of surface soil water and declining or flat responses beyond that threshold, suggesting a shift from water- to energy-limited transpiration. In contrast, drier areas exhibited limited transpiration sensitivity to soil water conditions and minimal trait-based variation in VPD responses, indicating supply-limited conditions. Despite higher wood density trees being generally more resilient, this study shows diverse tree drought resilience, prompting further investigation into the specific traits and dynamics between environmental variables in regulating transpiration and other physiological processes in trees.

Drought↗

Water availability modulates maximum canopy heights of low-elevation Amazonian second-growth forests

Tropical second-growth forests of the Amazon sequester large amounts of carbon and are important carbon sinks, contributing substantially to climate change mitigation, biodiversity conservation, and providing crucial ecosystem services. Deforestation due to selective logging and shifting cultivation is expanding second-growth forest areas in tropical forest regions, which if well managed, regenerate rapidly over time. Maximum forest canopy height is an important metric of biomass and carbon accumulation in second-growth forests and is strongly influenced by water availability. The water limitation hypothesis explains the positive influence of water availability on maximum tree heights and has been examined and demonstrated at a small-scale using field data, and at a global scale, with limited accuracy, using remote sensing data in tropical ecosystems. However, this hypothesis concerning maximum canopy height has not been much studied at regional and national scales for tropical second-growth forests. In this study, we leveraged NASA GEDI spaceborne lidar data across the Brazilian Amazon and derived second-growth forest relative height metrics for delineating the influence of water availability, second-growth forest age, and topographic elevation on maximum canopy height. Water availability was found to significantly influence the maximum canopy height of second-growth forest trees, of age range from 30 to 35 years, at elevations less than 500 m and maximum precipitation thresholds of 1500 mm. Our results indicate that changing precipitation patterns or increased drought conditions under different climate change regimes could impact forest structure, plant communities, ecosystem functioning, and carbon sequestration capabilities of tropical second-growth forests in the Amazon.

Carbon sequestration↗

Turbulence regimes in the nocturnal roughness sublayer: Interaction with deep convection and tree mortality in the Amazon

We investigated the influence of seasonality and proximity to the forest canopy on nocturnal turbulence regimes in the roughness sublayer of a Central Amazon forest. Since convective systems of different scales are common in this region, we also analyzed the effect of extreme wind gusts (propagated from convective downdrafts) on the organization of the turbulence regimes, and their potential to cause the mortality of canopy trees. Our data include high-frequency winds, temperature and ozone concentration at different heights during the dry and wet seasons of 2014. In addition, we used critical wind-speed data derived from a tree-winching experiment and a modeling study conducted in the same study site. Two different turbulence regimes were identified at three heights above the canopy: a weakly stable (WS) and a very stable regime (VS). The threshold wind speeds that mark the transition between turbulence regimes were larger during the dry season and increased as a function of the height above the canopy. The turbulent fluxes of sensible heat and momentum during the WS accounted for 88% of the entire nighttime flux. Downdrafts occurred only in the WS and favored a fully coupled state of wind flow along the canopy profile. The destructive potential of winds was four times higher than on nights without downdrafts.

54 ENVIRONMENTAL SCIENCES↗

Structural complexity biases vegetation greenness measures

Vegetation ‘greenness’ characterized by spectral vegetation indices (VIs) is an integrative measure of vegetation leaf abundance, biochemical properties and pigment composition. Surprisingly, satellite observations reveal that several major VIs over the US Corn Belt are higher than those over the Amazon rainforest, despite the forests having a greater leaf area. This contradicting pattern underscores the pressing need to understand the underlying drivers and their impacts to prevent misinterpretations. Here we show that macroscale shadows cast by complex forest structures result in lower greenness measures compared with those cast by structurally simple and homogeneous crops. The shadow-induced contradictory pattern of VIs is inevitable because most Earth-observing satellites do not view the Earth in the solar direction and thus view shadows due to the sun–sensor geometry. The shadow impacts have important implications for the interpretation of VIs and solar-induced chlorophyll fluorescence as measures of global vegetation changes. For instance, a land-conversion process from forests to crops over the Amazon shows notable increases in VIs despite a decrease in leaf area. In conclusion, our findings highlight the importance of considering shadow impacts to accurately interpret remotely sensed VIs and solar-induced chlorophyll fluorescence for assessing global vegetation and its changes.

60 APPLIED LIFE SCIENCES↗

Hot droughts in the Amazon provide a window to a future hypertropical climate

Tropical forests represent the warmest and wettest of Earth’s biomes, but with continued anthropogenic warming, they will be pushed to climate states with no current analogue. Droughts in the tropics are already becoming more intense as they occur at successively higher temperatures. Here, in this study, we synthesize multiple datasets to assess the effects of hot droughts on a central Amazon forest. First, a more than 30-year record of annually resolved forest demographic data from a selective logging experiment showed higher tree mortality during intense droughts, particularly among fast-growing pioneer species with low wood density. Second, analysis of ecophysiological field measurements from the 2015 and 2023 El Niño droughts identified a soil moisture threshold beyond which transpiration rates rapidly declined. As rainless days beyond this threshold continued, drought conditions intensified, increasing the potential for tree mortality from hydraulic failure and carbon starvation. Third, analyses from the Coupled Model Intercomparison Project Phase 6 demonstrated that under high-emission scenarios, a large area of tropical forest will shift to a hotter ‘hypertropical’ climate by 2100. Last, under a hypertropical climate, temperature and moisture conditions during typical dry season months will more frequently exceed identified drought mortality thresholds, elevating the risk of forest dieback. Present-day hot droughts are harbingers of this emerging climate, offering a window for studying tropical forests under expected extreme future conditions.

drought↗

Deforestation triggering irreversible transition in Amazon hydrological cycle

The Amazon is hypothesized to reach an irreversible ‘tipping point’ when deforestation slows the hydrological cycle sufficiently that tropical forest ecosystems cannot be sustained. However, inception of such a tipping point has not been supported by observations and the relevant links between deforestation and atmospheric moisture recycling are poorly understood. Here we show that reduction in evapotranspiration from 20 years of deforestation dried the atmosphere persistently and caused moisture decoupling, i.e. an opposite sign of moisture change between the lower and middle troposphere. Increased deforestation exacerbated the lower troposphere drying and caused it to penetrate deeper into the middle troposphere in the dry and transition seasons over monsoon forests and savannas. Deforestation induced warming-enhanced buoyant updrafts, elevated hot and dry air and thereby reduced downward mixing of water supplies from the tropical Atlantic that normally moisten the Amazon forests. The severe atmospheric desiccation in the southern and eastern Amazon cannot be compensated by enhanced water supplies from the Atlantic Ocean, demonstrating an irreversible transition in Amazon hydrological cycle exacerbated by rapid deforestation. The more recent drying through the seasons over rainforests and during the wet season over the transition zones from rainforests to monsoon forests and savannas, however, suggests a window of opportunity for preventing ecosystem collapse with forest conservation.

54 ENVIRONMENTAL SCIENCES↗

The Latent Dirichlet Allocation model applied to airborne LiDAR data: A case study on mapping forest degradation associated with fragmentation and fire in the Amazon region

1. LiDAR data are being increasingly used to provide a detailed characterization of the vertical profile of forests. This characterization enables the generation of new insights on the influence of environmental drivers and anthropogenic disturbances on forest structure as well as on how forest structure influences important ecosystem functions and services. Unfortunately, extracting information from LiDAR data in a way that enables the spatial visualization of forest structure, as well as its temporal changes, is challenging due to the high dimensionality of these data. 2. In this study, we show how the Latent Dirichlet Allocation model applied to LiDAR data (LidarLDA) can be used to identify forest structural types and how the relative abundance of these forest types changes throughout the landscape. The code to fit this model is made available through the open-source r package LidarLDA in github. We illustrate the use of LidarLDA both with simulated data and data from a large-scale fire experiment in the Brazilian Amazon region. 3. Using simulated data, we demonstrate that LidarLDA accurately identifies the number of forest types as well as their spatial distribution and absorptance probabilities. For the empirical data, we found that LidarLDA detects both landscape-level patterns in forest structure as well as the strong interacting effect of fire and forest fragmentation on forest structure based on the experimental fire plots. More specifically, LidarLDA reveals that proximity to forest edge exacerbates the impact of fires, and that burned forests remain structurally different from unburned areas for at least 7 years, even when burned only once. Importantly, LidarLDA generates insights on the 3D structure of forest that cannot be obtained using more standard approaches that just focus on top-of-the-canopy information (e.g. canopy height models based on LiDAR data). 4. By enabling the mapping of forest structure and its temporal changes, we believe that LidarLDA will be of broad utility to the ecological research community.

54 ENVIRONMENTAL SCIENCES↗

Amazon drought resilience – emerging results point to new empirical needs

Will climate change turn tropical forests from a carbon sink to a source (Pan et al., 2011)? Tropical forests cycle more carbon, water, and energy than any other biome (Bonan, 2008). The Amazon is the largest tropical forest, spanning one-third of South America, and any perturbation in its function has repercussions on the global climate. Mild and severe droughts in the Amazon are predicted to double and triple in area, respectively, by 2100, while the area under wet extremes will increase (Duffy et al., 2015). Our understanding of the role of plants' hydrological environments in determining their drought response, however, is limited (Chitra-Tarak et al., 2018, 2021). An important review by Costa et al. (2023; pp. 714–733) published in this issue of New Phytologist highlights that shallow-water table (WT) forests constitute c. 50% of the Amazon and may act as hydrological refugia during droughts, yet they have been neglected in Amazon forest research to date. Emerging results suggest that the shallow-WT forests that dominate the Amazon basin may increase in productivity under moderate droughts due to relief from hypoxia, suggesting a potential buffer from drought relative to deeper WT forests (Fig. 1). During severe droughts, however, shallow-WT forests may be vulnerable to collapse due to drought-intolerant traits. Addressing the underrepresentation of relatively drought-resilient shallow-WT forests in the Amazon's forest inventories may help resolve the much-debated incongruence in recent studies of the Amazon's drought resilience. Data from forest inventories (biased toward deep-WT forests) indicate that the Amazon's carbon sink is declining, partially because of drought impacts (Phillips et al., 2009; Brienen et al., 2015), whereas basin-wide satellite-based measures of gross primary productivity, which include shallow-WT forests, indicate varied regional responses to droughts (Saleska et al., 2007; Brando et al., 2010). Costa et al.'s (2023) descriptive insights of the intricate balance of geology, topography, hydrology, vegetation, and drought on ecosystem function, as well as their conceptual predictive framework, are useful for developing new empirical research in these understudied ecosystems and improving Earth system models.

54 ENVIRONMENTAL SCIENCES↗

Increased Occurrence of Large–Scale Windthrows Across the Amazon Basin

Convective storms with strong downdrafts create windthrows: snapped and uprooted trees that locally alter the structure, composition, and carbon balance of forests. Comparing Landsat imagery from subsequent years, we documented temporal and spatial variation in the occurrence of large (≥30 ha) windthrows across the Amazon basin from 1985 to 2020. Over 33 individual years, we detected 3179 large windthrows. Windthrow density was greatest in the central and western Amazon regions, with ~33% of all events occurring in ~3% of the monitored area. Return intervals for large windthrows in the same location of these “hotspot” regions are centuries to millennia, while over the rest of the Amazon they are >10,000 years. Our data demonstrate a nearly 4–fold increase in windthrow number and affected area between 1985 (78 windthrows and 6,900 ha) and 2020 (264 events and 32,170 ha), with more events of >500 ha size since 1990. Such extremely large events (>500 ha up to 2,543 ha) are responsible for interannual variation in the overall median (84 ± 5.2 ha; ±95% CI) and mean (147 ± 13 ha) windthrow area, but we did not find significant temporal trends in the size distribution of windthrows with time. Our results document increased damage from convective storms over the past 40 years in the Amazon, filling a gap in temporal records for tropical regions. Our publicly accessible large windthrow database provides a valuable tool for exploring dynamic conditions leading to damaging storms and their ecological impact on Amazon forests.

54 ENVIRONMENTAL SCIENCES↗

Windthrow characteristics and their regional association with rainfall, soil, and surface elevation in the Amazon

Abstract Windthrows (trees uprooted and broken by winds) are common across the Amazon. They range in size from single trees to large gaps that lead to changes in forest dynamics, composition, structure, and carbon balance. Yet, the current understanding of the spatial variability of windthrows is limited. By integrating remote sensing data and geospatial analysis, we present the first study to examine the occurrence, area, and direction of windthrows and the control that environmental variables exert on them across the whole Amazon. Windthrows are more frequent and larger in the northwestern Amazon (Peru and Colombia), with the central Amazon (Brazil) being another hot spot of windthrows. The predominant direction of windthrows is westward. Rainfall, surface elevation, and soil characteristics explain the variability (20%–50%) of windthrows but their effects vary regionally. A better understanding of the spatial dynamics of windthrows will improve understanding of the functioning of Amazon forests.

54 ENVIRONMENTAL SCIENCES↗

An ecological niche model to predict the geographic distribution of Haemagogus janthinomys, Dyar, 1921 a yellow fever and Mayaro virus vector, in South America

Yellow fever virus (YFV) has a long history of impacting human health in South America. Mayaro virus (MAYV) is an emerging arbovirus of public health concern in the Neotropics and its full impact is yet unknown. Both YFV and MAYV are primarily maintained via a sylvatic transmission cycle but can be opportunistically transmitted to humans by the bites of infected forest dwelling Haemagogus janthinomys Dyar, 1921. To better understand the potential risk of YFV and MAYV transmission to humans, a more detailed understanding of this vector species’ distribution is critical. This study compiled a comprehensive database of 177 unique Hg . janthinomys collection sites retrieved from the published literature, digitized museum specimens and publicly accessible mosquito surveillance data. Covariate analysis was performed to optimize a selection of environmental (topographic and bioclimatic) variables associated with predicting habitat suitability, and species distributions modelled across South America using a maximum entropy (MaxEnt) approach. Our results indicate that suitable habitat for Hg . janthinomys can be found across forested regions of South America including the Atlantic forests and interior Amazon.

60 APPLIED LIFE SCIENCES↗

Near-canopy horizontal concentration heterogeneity of semivolatile oxygenated organic compounds and implications for 2-methyltetrols primary emissions

Semivolatile oxygenated organic compounds (SV-OVOCs) are important atmospheric species, in particular for the production and chemistry of atmospheric particulate matter and related impacts on air quality and climate. In this study, SV-OVOCs were collected in the horizontal plane of the roughness layer over the tropical forest in the central Amazon during the wet season of 2018. A sampler mounted to a copter-type, hovering unmanned aerial vehicle was used. Underlying the collection region, a plateau forest transitioned into a slope forest across several hundred meters. The concentrations of pinonic and pinic acids, which are monoterpene oxidation products, had no statistical difference over the two forests. By comparison, across the study period, differences in the concentration of 2-methyltetrols, which are products of isoprene oxidation, ranged from -70% to +480% over the two forests. The chemical lifetime of 2-methyltetrols in the atmosphere is sufficiently long that heterogeneity in the isoprene emission rate from the two forests followed by atmospheric oxidation does not explain the concentration heterogeneity of 2-methyltetrols. Standing waves and local meteorology also cannot account for the heterogeneity. Of the possibilities considered, the most plausible explanation is the direct emission from the forest of 2-methyltetrols produced through biological processes within the plants. Under this explanation, the rate of direct SV-OVOC emissions should be modulated by forest type and related environmental stressors. Direct emissions of SV-OVOCs should be more broadly considered for constraining and improving models of atmospheric composition, transport, and chemistry over tropical forests.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Vismia guianensis leaf age physiology in Manaus, Brazil, from July 2014 - July 2015

This data package contains net photosynthesis rates and methanol and isoprene leaf emissions as a function of light, temperature, and leaf age. Data was collected from the species V. guianensis at the NGEE Tropics site in Manaus, Brazil (BR-Ma2). This data is used in the publication "Methanol and Isoprene Emissions from the Fast Growing Tropical Pioneer Species Vismia guianensis (Aubl.) Pers. (Hypericaceae) in the Amazon Basin." Isoprene (Is) emissions by plants represent a loss of carbon and energy resources leading to the initial hypothesis that fast growing pioneer species in secondary tropical forests allocate carbon primarily to growth at the expense of isoprenoid defenses. In this study, we quantified leaf isoprene and methanol emissions from the abundant pantropical pioneer tree species Vismia guianensis and ambient isoprene concentrations above a diverse secondary forest in the central Amazon. See the below field, Dataset References, for the full citation. Included in the data package is a zip file with six datasets, three in Excel format and additional versions in CSV format. Also included are the figures from the related publication.

54 ENVIRONMENTAL SCIENCES↗

Soil moisture thresholds explain a shift from light-limited to water-limited sap velocity in the Central Amazon during the 2015–16 El Niño drought

Transpiration is often considered to be light- but not water-limited in humid tropical rainforests due to abundant soil water, even during the dry seasons. The record-breaking 2015–16 El Niño drought provided a unique opportunity to examine whether transpiration is constrained by water under severe lack of rainfall. We measured sap velocity, soil water content, and meteorological variables in an old-growth upland forest in the Central Amazon throughout the 2015–16 drought. We found a rapid decline in sap velocity (-38 ± 21%, mean ± SD.) and in its temporal variability (-88%) during the drought compared to the wet season. Such changes were accompanied by a marked decline in soil moisture and an increase in temperature and vapor pressure deficit. Sap velocity was largely limited by net radiation during the wet and normal dry seasons; however, it shifted to be primarily limited by soil moisture during the drought. The threshold in which sap velocity became dominated by soil moisture was at 0.33 m 3 m -3 (around -150 kPa in soil matric potential), below which sap velocity dropped steeply. Our study provides evidence for a soil water threshold on transpiration in a moist tropical forest, suggesting a shift from light limitation to water limitation under future climate characterized by increased temperature and an increased frequency, intensity, duration and extent of extreme drought events.

54 ENVIRONMENTAL SCIENCES↗

Growth, mortality, wood density, biomass data from BIONTE inventories in Manaus, Brazil

BIONTE (BIOmass and NuTrient Experiment) is a selective logging experiment established at the Experimental Station of Tropical Forestry (EEST, aka “ZF2”) field research station in the mid 1980s in the central Amazon (Higuchi et al. 1997, Amaral et al. 2019). Led by the National Institute for Amazon Research (INPA) in Brazil, the project aimed at assessing the effects of logging intensity on forest dynamics and enabling the creation of a model of forest management for the Central Amazon. The experiment included three levels of increasing selective logging intensity and controls, with 1 hectare sample plots (12 total) located at the center of 4 hectare treatment plots. The site’s Köppen classification is tropical rainforest (Af), characterized by high temperatures and humidity, with mean annual temperatures around 27 ℃ and mean annual precipitation around 2200 mm of rain. The vegetation has a high floristic diversity, the soils of the region are poor in nutrients, and the topography is characterized by plateaus (where BIONTE is located), and also valley bottoms and slopes. The inventory (growth and mortality) and biomass data included here covers the 1990 to 2019 period, with wood density being averaged from existing datasets. This dataset includes a data file in .csv file format and a .txt file, BIONTE_mortality-rates_headers.txt, that provides descriptions for the data file headers.

54 ENVIRONMENTAL SCIENCES↗

Integrating high resolution drone imagery and forest inventory to distinguish canopy and understory trees and quantify their contributions to forest structure and dynamics

Tree growth and survival differ strongly between canopy trees (those directly exposed to overhead light), and understory trees. However, the structural complexity of many tropical forests makes it difficult to determine canopy positions. The integration of remote sensing and ground-based data enables this determination and measurements of how canopy and understory trees differ in structure and dynamics. Here we analyzed 2 cm resolution RGB imagery collected by a Remotely Piloted Aircraft System (RPAS), also known as drone, together with two decades of bi-annual tree censuses for 2 ha of old growth forest in the Central Amazon. We delineated all crowns visible in the imagery and linked each crown to a tagged stem through field work. Canopy trees constituted 40% of the 1244 inventoried trees with diameter at breast height (DBH) > 10 cm, and accounted for ~70% of aboveground carbon stocks and wood productivity. The probability of being in the canopy increased logistically with tree diameter, passing through 50% at 23.5 cm DBH. Diameter growth was on average twice as large in canopy trees as in understory trees. Growth rates were unrelated to diameter in canopy trees and positively related to diameter in understory trees, consistent with the idea that light availability increases with diameter in the understory but not the canopy. The whole stand size distribution was best fit by a Weibull distribution, whereas the separate size distributions of understory trees or canopy trees > 25 cm DBH were equally well fit by exponential and Weibull distributions, consistent with mechanistic forest models. The identification and field mapping of crowns seen in a high resolution orthomosaic revealed new patterns in the structure and dynamics of trees of canopy vs. understory at this site, demonstrating the value of traditional tree censuses with drone remote sensing.

59 BASIC BIOLOGICAL SCIENCES↗

Disentangling the Effects of Vapor Pressure Deficit and Soil Water Availability on Canopy Conductance in a Seasonal Tropical Forest During the 2015 El Niño Drought

In land models used in Earth system models, vegetation water stress is heuristically related to soil water matric potential, and root water uptake in soil layers is simply proportional to root density to meet the transpiration demand. The efficiency of roots utilizing deeper soil water under water stress cannot be well represented, but it is of critical importance for transpiration regulation and carbon flux. To improve modeling of tropical forest response to drought, a plant hydrodynamic scheme (HYDRO), adapted from a trait-based model in the Functionally Assembled Terrestrial Ecosystem Simulator (FATES), has been implemented in the Energy Exascale Earth System Model (E3SM) Land Model (ELM) to represent the hydraulic dynamics and root water uptake strategy. The adapted model conforms to the big-leaf photosynthesis model in ELM. Vegetation water stress in the model is a function of prognostic leaf water potential and is used to regulate stomatal conductance. Point simulations were performed at two tropical forest sites in the Amazon and Panama. Model sensitivity to the numerical solver, plant hydraulic traits, soil properties, and atmospheric forcing has been evaluated. Our results showed that, even though water stress factors calculated at our study sites using HYDRO are comparable to that simulated by the heuristic water stress model, soil moisture prediction is improved with soil water redistributions through roots. Simulated results using HYDRO is not as sensitive to plant hydraulic traits measured for different tree species as to the soil properties and atmospheric forcing. Simplified numerical solver may cause inaccuracy as to where roots uptake water. This study suggests that plant hydrodynamic representation is critical for improved simulations of soil moisture under water stresses.

54 ENVIRONMENTAL SCIENCES↗

Amazon windthrow disturbances are likely to increase with storm frequency under global warming

Abstract Forest mortality caused by convective storms (windthrow) is a major disturbance in the Amazon. However, the linkage between windthrows at the surface and convective storms in the atmosphere remains unclear. In addition, the current Earth system models (ESMs) lack mechanistic links between convective wind events and tree mortality. Here we find an empirical relationship that maps convective available potential energy, which is well simulated by ESMs, to the spatial pattern of large windthrow events. This relationship builds connections between strong convective storms and forest dynamics in the Amazon. Based on the relationship, our model projects a 51 ± 20% increase in the area favorable to extreme storms, and a 43 ± 17% increase in windthrow density within the Amazon by the end of this century under the high-emission scenario (SSP 585). These results indicate significant changes in tropical forest composition and carbon cycle dynamics under climate change.

54 ENVIRONMENTAL SCIENCES↗