Buffering activity of algal cells and its effect on cell division 1, 2
Buffering of algae - cell division of Chlorella
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Buffering of algae - cell division of Chlorella
Organic synthesis of high temperature chlorella separated into small or large cells by fractional centrifugation
Photosynthetic gas exchange experiment using dwarf mouse and illuminated suspension of Chlorella ellipsoidea
Synthetic activity during nonsynchronized growth chlorella, separated into age groups by fractional centrifugation
Photosynthetic activity in small and large cells of chlorella pyrenoidosa, 7-11-05
Physiology of Chlorella species for application to space exploration life support systems
Buffering capacity of substances liberated by Chlorella expressed in terms of van Slyke buffer index, noting changes in pH
High respiration rate of Chlorella maintained by blue-green light
Continuous synchronous culture of photosynthetic microorganism Chlorella cultivated in illuminated and dark stirred tanks
Secretory activity of algal Chlorella cells effect on buffering characteristics of Tris or sodium citrate-citric acid suspending fluid
The Russian experience with the bioregenerative life support system Bios-3 at Krasnoyarsk, Siberia, is reviewed. A brief review of other bioregenerative systems examines Biosphere 2 in Oracle, Arizona, and the Bios-1 and Bios-2 systems that preceded Bios-3. Physical details of the Bios-3 facility are provided. The use of Chlorella and higher plants for gas exchange is examined. Long-term studies of human habitation are discussed. Other topics include microflora in Bios-3, the theory of closed systems, and problems for the future.
Linear density gradients in isopycnic centrifugation technique for selecting synchronous daughter cells from asynchronous Chlorella cultures
The primary objective of this research is to obtain fundamental data concerning the growth and metabolism of the unicellular green algae. These organisms are most likely to provide biological oxygen and a food source for space crews. Biochemical conversions, chemical composition, and cell growth and division are discussed. Chlorella sorokiniana is emphasized.
Data are presented which give the specific photosynthetic rate and the specific utilization rates of urea and carbon dioxide as functions of specific growth rate for Chlorella. A mathematical model expresses a set of mass balance relations between biotic and environmental materials. Criteria of validity are used to test this model. Predictive procedures are complemented by a particular model of microbial growth. Methods are demonstrated for predicting substrate utilization rates, production rates of extracellular metabolites, growth limiting conditions, and photosynthetic quotients from propagator variables.
Various models of biological human life support systems are surveyed. Biological structures, dimensions, and functional parameters of man-chlorella-microorganism models are described. Significant observations and the results obtained from these models are reported.
The production of phytotoxic air pollutants by cultures of Chlorella vulgaris and Euglena gracilis is considered. Algal and plant culture systems, a fumigation system, and ethylene, ethane, cyanide, and nitrogen oxides assays are discussed. Bean, tobacco, mustard green, cantaloupe and wheat plants all showed injury when fumigated with algal gases for 4 hours. Only coleus plants showed any resistance to the gases. It is found that a closed or recycled air effluent system does not produce plant injury from algal air pollutants.
Euglena gracilis and Chlorella vulgaris cultures and toxic gas identification are discussed.
The objectives were to develop a trap system for isolating fractions of volatile algal phytotoxin and to characterize the major components of the isolated phytotoxin fractions. A bioassay using Phaseolus vulgaris seedlings was developed to aid in investigating the properties of the phytotoxin produced by cultures of Euglena gracilis var. bacillaris and Chlorella vulgaris. Two traps were found, 1.0 M hydrochloric acid and 0 C, which removed the phytotoxin from the algal effluent and which could be treated to release that phytotoxin as judged with the bioassay procedure. It was also determined that pretraps of 1.0 M sodium hydroxide and 1.0 M potassium biocarbonate could be used without lowering the phytotoxin effect. Ammonia was identified in trap solutions by ninhydrin reaction, indophenol reaction and derivatization with dansyl chloride and phenylisothiocyanate. Ammonia at the gaseous concentrations detected was found to have the same effects in the bioassay system as the volatile phytotoxin. It is possible that other basic, nitrogen containing compounds which augment the effects of ammonia were present at lower concentrations in the algal effluent.