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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 73 records · Page 4

Planetary Biology and Microbial Ecology: Molecular Ecology and the Global Nitrogen cycle

This report summarizes the results of the Planetary Biology and Molecular Ecology's summer 1991 program, which was held at the Marine Biological Laboratory in Woods Hole, Massachusetts. The purpose of the interdisciplinary PBME program is to integrate, via lectures and laboratory work, the contributions of university and NASA scientists and student interns. The goals of the 1991 program were to examine several aspects of the biogeochemistry of the nitrogen cycle and to teach the application of modern methods of molecular genetics to field studies of organisms. Descriptions of the laboratory projects and protocols and abstracts and references of the lectures are presented.

Nealson, Molly Stone↗

Chemical characterization of some aerobic liquids in CELSS

Untreated aqueous soybean and wheat leachate and aerobically treated wheat leachate prepared from crop residues that are produced as a component of the Controlled Ecological Life Support System program designed to support long duration space missions were compared, and a general chemical characterization was accomplished. Solid phase extraction and high performance liquid chromatography were used to accomplish comparisons based on chromatographic and ultraviolet absorption properties of the components that are present. Specific compounds were not identified; however, general composition related to the initial presence of phenol-like compounds and their disappearance during aerobic treatment was explored.

Madsen, Brooks C.↗

CELSS system control: issues, methods, and directions

In the general control perspective, the CELSS concept implies a very complex system and presents challenges at every level. These challenges are generated by: (1) the prospect that the system will be inherently unstable, (2) the prospective difficulty of establishing an adequate mathematical model of the system for the purpose of control law synthesis (dimensionality is high, and the dynamics and interactive processes of some of the subsystems are not understood well), (3) assuring control law robustness (assuring that the resulting control law(s) will be effective over the domain of the specified uncertainties), (4) hardware realization of the control law, (5) hardware system robustness ("fault tolerance") and (6) achieving the logistics of the automation (or "management") aspects of the problem. A suggested organization of the problem, a sketch of the issues related to perceived difficulties, a commentary/evaluation of the issues, a review of methods available to address the issues, and a suggested strategy to address the broad CELSS systems control problem are presented.

NASA Center ARC↗

Space Research Fortifies Nutrition Worldwide

NASA's Controlled Ecological Life Support Systems program attempted to address basic needs of crews, meet stringent payload and power usage restrictions, and minimize space occupancy, by developing living, regenerative ecosystems that would take care of themselves and their inhabitants. An experiment from this program evolved into one of the most widespread NASA spinoffs of all time-a method for manufacturing an algae-based food supplement that provides the nutrients previously only available in breast milk. Martek Biosciences Corporation, in Columbia, Maryland, now manufactures this supplement, and it can be found in over 90 percent of the infant formulas sold in the United States, as well as those sold in over 65 other countries. With such widespread use, the company estimates that over 24 million babies worldwide have consumed its nutritional additives.

Source record↗

Predictive Risk Mapping of Schistosomiasis in Madagascar Using Ecological Niche Modeling and Precision Mapping

Schistosomiasis is a neglected tropical disease (NTD) found throughout tropical and subtropical Africa. In Madagascar, the condition is widespread and endemic in 74% of all administrative districts in the country. Despite the significant burden of the disease, high-resolution risk maps have yet to be produced to guide national control programs. This study used an ecological niche modeling (ENM) and precision mapping approach to estimate environmental suitability and disease transmission risk. The results show that suitability for schistosomiasis is widespread and covers 264,781 km 2 (102,232 sq miles). Covariates of significance to the model were the accessibility to cities, distance to water, enhanced vegetation index (EVI), annual mean temperature, land surface temperature (LST), clay content, and annual precipitation. Disease transmission risk is greatest in the central highlands, tropical east coast, arid-southwest, and northwest. An estimated 14.9 million people could be at risk of schistosomiasis; 11.4 million reside in rural areas, while 3.5 million are in urban areas. This study provides valuable insight into the geography of schistosomiasis in Madagascar and its potential risk to human populations. Because of the focal nature of the disease, these maps can inform national surveillance programs while improving understanding of areas in need of medical interventions.

60 APPLIED LIFE SCIENCES↗

Application of photosynthetic N2-fixing cyanobacteria to the CELSS program

Commercially available air lift fermentors were used to simultaneously monitor biomass production, N2-fixation, photosynthesis, respiration, and sensitivity to oxidative damage during growth under various nutritional and light regimes, to establish a data base for the integration of these organisms into a Closed Ecological Life Support System (CELSS) program. Certain cyanobacterial species have the unique ability to reduce atmospheric N2 to organic nitrogen. These organisms combine the ease of cultivation characteristics of prokaryotes with the fully developed photosynthetic apparatus of higher plants. This, along with their ability to adapt to changes in their environment by modulation of certain biochemical pathways, make them attractive candidates for incorporation into the CELSS program.

Packer, L.↗

Un-Numbered Operational Areas PRL 229 Railroad Tie Disposal Area Pond Confirmation Sampling Report

This Confirmatory Sampling (CS) Report (CSR) was prepared for the National Aeronautics and Space Administration (NASA), Kennedy Space Center (KSC), Florida in accordance with the CS Work Plan (CSWP) (HGL, 2022a). The CSR summarizes investigation activities performed on the Railroad Tie Disposal Area (RTDA) of the Un-Numbered Operational Areas (UNOA), Potential Release Location (PRL) 229 located at KSC. HydroGeoLogic, Inc. (HGL) prepared this report under Contract Number 80KSC019D0012. The purpose of the CSR is to provide information to evaluate the level of potential contamination in surface water and sediment samples collected from the RTDA PRL 229 Pond and determine if contaminants pose unacceptable risks to ecological receptors. The CSR provides analytical data of 10 sets of surface water and sediment samples collected in 2021. Surface water samples were analyzed for volatile organic compounds (VOCs), semi-volatile organic compounds (SVOCs), polynuclear aromatic hydrocarbon (PAHs), metals, hardness, and salinity, while sediment samples were analyzed for metals, total organic carbon (TOC), SVOCs, PAHs, and total petroleum hydrocarbons (TPH). The CSR includes a Screening Level Ecological Risk Assessment (SLERA) conducted under KSC’s Resource Conservation and Recovery Act (RCRA) Corrective Action Program to determine if unacceptable risks to ecological receptors exist at PRL 229 Pond. Metals and PAHs were detected in surface water samples, including detections of antimony, arsenic, barium, calcium, copper, lead, magnesium, thallium, zinc, anthracene, benzo(a)anthracene, benzo(a)pyrene, benzo(b)fluoranthene, benzo(g,h,i)perylene, benzo(k)fluoranthene, chrysene, dibenzo(a,h)anthracene, fluoranthene, indeno(1,2,3-c,d)pyrene and pyrene. Based on measured salinity (1.03 to 1.21 parts per thousand), the pond was identified as freshwater. Sediment analytical results also contained detections of metals and PAHs, including antimony, arsenic, barium, beryllium, cadmium, chromium, copper, lead, mercury, nickel, selenium, thallium, zinc, anthracene benzo(a)anthracene, benzo(a)pyrene, benzo(b)fluoranthene, benzo(g,h,i)perylene, benzo(k)fluoranthene, chrysene, dibenzo(a,h)anthracene, fluoranthene, indeno(1,2,3-c,d)pyrene and pyrene. Potential risks to ecological receptors were evaluated in accordance with the SLERA Work Plan and the Decision Process Document for the Resource Conservation and Recovery Act (RCRA) Corrective Action Program at KSC (DPD) (Geosyntec, 2019), and using the 2021 sediment and surface water analytical results. Potential ecological receptors evaluated in the SLERA include the aquatic community, benthic invertebrates, aquatic birds, and aquatic mammals. The SLERA included Steps 2a through 2c of the Ecological Risk Assessment (ERA) process in accordance with the DPD. Using multiple lines of evidence, the SLERA concluded that many of the detected analytes are present at concentrations similar to those in background or reference locations at KSC, and/or at concentrations that would not pose a significant risk to ecological communities. There are no screening values available for the avian community, and PAHs are bioaccumulative compounds. For this reason, food web modeling was completed as a supplement to Step 2c to provide a quantitative evaluation of this exposure pathway. Food web modeling indicates that PAHs are unlikely to pose a risk to birds exposed to site sediment and surface water. Based on the results of the SLERA, the contaminants in sediment and surface water pose no to minimal risk to the ecological communities that could live or forage at the site. Based on the results of the SLERA, no further study is recommended for the RTDA Pond at PRL 229.

Confirmation Sampling↗

Lighting the Way to the Stars

NASA has long recognized the importance of biological life-support systems to remove carbon dioxide, generate oxygen, purify water, and produce food for long-duration space missions. Experiments to understand the effects of the space environment on plant development have been performed since early days of the space program. In the late 1970s, NASA sponsored a series of workshops to identify issues associated with developing a sustainable, biological life-support system for long-duration space missions. Based on findings from these workshops, NASA's Controlled Ecological Life Support Systems (CELSS) program began funding research at university and field centers to systematically conduct the research identified in those workshops. Key issues were the necessity to reduce mass, power/energy requirements, and volume of all components.

Way To↗

Controlled Ecological Life Support System: Research and Development Guidelines

Results of a workshop designed to provide a base for initiating a program of research and development of controlled ecological life support systems (CELSS) are summarized. Included are an evaluation of a ground based manned demonstration as a milestone in CELSS development, and a discussion of development requirements for a successful ground based CELSS demonstration. Research recommendations are presented concerning the following topics: nutrition and food processing, food production, waste processing, systems engineering and modelling, and ecology-systems safety.

Mason, R. M.↗

Controlled Ecological Life Support System

The NASA CELLS program is based upon the integration of biological and physiochemical processes in order to produce a system that will produce food, a breathable atmosphere, and potable water from metabolic and other wastes. The CELSS concept is described and a schematic system diagram is provided. Central to the CELSS concept is the Plant Growth Chamber, where green plant photosynthesis produces food, and aids in the production of oxygen and water. Progress to date at the Breadboard Facility at the Kennedy Space Center is summarized. The Breadboard Facility will implement the basic techniques and processes required for a CELSS based on photosynthetic plant growth in a ground-based system of practical size and results will be extrapolated to predict the performance of a full-sized system. Current available technology and near-future forecasts for plant growth techniques (focusing on maximum productivity), food sources (to select optimal CELSS plants), and waste management and contaminant control are discussed.

Averner, Maurice M.↗

Coassembly and binning of a twenty-year metagenomic time-series from Lake Mendota

Abstract The North Temperate Lakes Long-Term Ecological Research (NTL-LTER) program has been extensively used to improve understanding of how aquatic ecosystems respond to environmental stressors, climate fluctuations, and human activities. Here, we report on the metagenomes of samples collected between 2000 and 2019 from Lake Mendota, a freshwater eutrophic lake within the NTL-LTER site. We utilized the distributed metagenome assembler MetaHipMer to coassemble over 10 terabases (Tbp) of data from 471 individual Illumina-sequenced metagenomes. A total of 95,523,664 contigs were assembled and binned to generate 1,894 non-redundant metagenome-assembled genomes (MAGs) with ≥50% completeness and ≤10% contamination. Phylogenomic analysis revealed that the MAGs were nearly exclusively bacterial, dominated by Pseudomonadota (Proteobacteria, N = 623) and Bacteroidota (N = 321). Nine eukaryotic MAGs were identified by eukCC with six assigned to the phylum Chlorophyta. Additionally, 6,350 high-quality viral sequences were identified by geNomad with the majority classified in the phylum Uroviricota. This expansive coassembled metagenomic dataset provides an unprecedented foundation to advance understanding of microbial communities in freshwater ecosystems and explore temporal ecosystem dynamics.

59 BASIC BIOLOGICAL SCIENCES↗

Ion-exchange chromatography separation applied to mineral recycle in closed systems

As part of the controlled ecological life support system (CELSS) program, a study is being made of mineral separation on ion-exchange columns. The purpose of the mineral separation step is to allow minerals to be recycled from the oxidized waste products of plants, man, and animals for hydroponic food production. In the CELSS application, relatively large quantities of minerals in a broad concentration range must be recovered by the desired system, rather than the trace quantities and very low concentrations treated in analytical applications of ion-exchange chromatography. Experiments have been carried out to assess the parameters pertinent to the scale-up of ion-exchange chromatography and to determine feasibility. Preliminary conclusions are that the column scale-up is in a reasonable size range for the CELSS application. The recycling of a suitable eluent, however, remains a major challenge to the suitability of using ion exchange chromatography in closed systems.

Ballou, E.↗

CELSS Transportation Analysis

Regenerative life support systems based on the use of biological material was considered for inclusion in manned spacecraft. Biological life support systems are developed in the controlled ecological life support system (CELSS) program. Because of the progress achieved in the CELSS program, it is determined which space missions may profit from use of the developing technology. Potential transportation cost savings by using CELSS technology for selected future manned space missions was evaluated. Six representative missions were selected which ranged from a low Earth orbit mission to those associated with asteroids and a Mars sortie. The crew sizes considered varied from four persons to five thousand. Other study parameters included mission duration and life support closure percentages, with the latter ranging from complete resupply of consumable life support materials to 97% closure of the life support system. The analytical study approach and the missions and systems considered, together with the benefits derived from CELSS when applicable are described.

Olson, R. L.↗

The global sulfur cycle

The results of the planetary biology microbial ecology's 1984 Summer Research Program, which examined various aspects of the global sulfur cycle are summarized. Ways in which sulfur flows through the many living and chemical species that inhabit the surface of the Earth were investigated. Major topics studied include: (1) sulfur cycling and metabolism of phototropic and filamentous sulfur bacteria; (2) sulfur reduction in sediments of marine and evaporite environments; (3) recent cyanobacterial mats; (4) microanalysis of community metabolism in proximity to the photic zone in potential stromatolites; and (5) formation and activity of microbial biofilms on metal sulfides and other mineral surfaces. Relationships between the global sulfur cycle and the understanding of the early evolution of the Earth and biosphere and current processes that affect global habitability are stressed.

Sagan, D.↗

Current concepts and future directions of CELSS

The components of a bioregenerative life-support system intended for use in space are described and the requirements for system control are discussed. Concepts of such systems include the use of higher plants and/or micro-algae as sources of oxygen, CO2 absorption, potable water, and food. In order to focus on the specific problem of reservoirs and buffers, bioregenerative life support in space is contrasted to terrestrial ecological concepts. Some of the future directions of the NASA CELSS (controlled ecological life-support system) program are outlined.

Macelroy, R. D.↗

Plant growth chamber M design

Crop production is just one of the many processes involved in establishing long term survival of man in space. The benefits of integrating higher plants into the overall plan was recognized early by NASA through the Closed Ecological Life Support System (CELSS) program. The first step is to design, construct, and operate a sealed (gas, liquid, and solid) plant growth chamber. A 3.6 m diameter by 6.7 m high closed cylinder (previously used as a hypobaric vessel during the Mercury program) is being modified for this purpose. The chamber is mounted on legs with the central axis vertical. Entrance to the chamber is through an airlock. This chamber will be devoted entirely to higher plant experimentation. Any waste treatment, food processing or product storage studies will be carried on outside of this chamber. Its primary purpose is to provide input and output data on solids, liquids, and gases for single crop species and multiple species production using different nutrient delivery systems.

Prince, R. P.↗

Controlled ecological life support systems for space habitats

The development of regenerative life support systems to meet the physiological requirements of humans in space is described. A review of previous research on regenerative systems is presented. NASA's Controlled Ecological Life Support System (CELSS) program, which is to develop an environment for a human space crew, is discussed. The table of physiological requirements of an average human and the logical system approach to planning a closed system created by Spurlock and Modell (1976, 1978) are examined. The weight of food and oxygen with respect to lift-off weight is investigated. The creation of the proper atmosphere for space, by balancing all the necessary parameters is studied. The need for a mineral and fluid balance and methods of maintaining it are analyzed. The required cooperation between physicians, physiologists, and nutritionists for the success of the CELSS program is discussed.

Buchanan, P.↗