High-Resolution Secondary Ion Mass Spectrometry Depth Profiling of Nanolayers
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Triclosan (TCS) is an antimicrobial agent commonly found in a variety of personal care products and cosmetics. TCS readily enters the environment through wastewater and is detected in human plasma, urine, and breast milk due to its widespread use. Studies have implicated TCS as a disruptor of thyroid and estrogen signaling; therefore, research examining the developmental effects of TCS is warranted. In this study, we used embryonic zebrafish to investigate the developmental toxicity and potential mechanism of action of TCS. Embryos were exposed to graded concentrations of TCS from 6 to 120 hours post-fertilization (hpf) and the concentration where 80% of the animals had mortality or morbidity at 120 hpf (EC{sub 80}) was calculated. Transcriptomic profiling was conducted on embryos exposed to the EC{sub 80} (7.37 μM). We identified a total of 922 significant differentially expressed transcripts (FDR adjusted P-value ≤ 0.05; fold change ≥ 2). Pathway and gene ontology enrichment analyses identified biological networks and transcriptional hubs involving normal liver functioning, suggesting TCS may be hepatotoxic in zebrafish. Tissue-specific gene enrichment analysis further supported the role of the liver as a target organ for TCS toxicity. We also examined the in vitro bioactivity profile of TCS reported by the ToxCast screening program. TCS had a diverse bioactivity profile and was a hit in 217 of the 385 assay endpoints we identified. We observed similarities in gene expression and hepatic steatosis assays; however, hit data for TCS were more concordant with the hypothesized CAR/PXR activity of TCS from rodent and human in vitro studies. - Highlights: • Triclosan is a common antimicrobial agent with widespread human exposure. • Exposure to the triclosan EC{sub 80} causes robust gene expression changes in zebrafish. • The liver may be a target organ of triclosan toxicity in embryonic zebrafish. • Triclosan disrupts normal liver functioning and development in embryonic zebrafish. • A summary of triclosan's bioactivity profile in the ToxCast program is discussed.
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A representative H-mode discharge from the National Spherical Torus eXperiment is studied in detail to utilize it as a basis for a time-evolving prediction of the electron temperature profile using an appropriate reduced transport model. The time evolution of characteristic plasma variables such as β{sub e}, ν{sub e}{sup ∗}, the MHD α parameter, and the gradient scale lengths of T{sub e}, T{sub i}, and n{sub e} were examined as a prelude to performing linear gyrokinetic calculations to determine the fastest growing micro instability at various times and locations throughout the discharge. The inferences from the parameter evolutions and the linear stability calculations were consistent. Early in the discharge, when β{sub e} and ν{sub e}{sup ∗} were relatively low, ballooning parity modes were dominant. As time progressed and both β{sub e} and ν{sub e}{sup ∗} increased, microtearing became the dominant low-k{sub θ} mode, especially in the outer half of the plasma. There are instances in time and radius, however, where other modes, at higher-k{sub θ}, may, in addition to microtearing, be important for driving electron transport. Given these results, the Rebut-Lallia-Watkins (RLW) electron thermal diffusivity model, which is based on microtearing-induced transport, was used to predict the time-evolving electron temperature across most of the profile. The results indicate that RLW does a good job of predicting T{sub e} for times and locations where microtearing was determined to be important, but not as well when microtearing was predicted to be stable or subdominant.
We present a comprehensive simulation study on the solid-liquid phase transition of the ionic liquid 1,3-dimethylimidazolium chloride in terms of the changes in the atomic structure and their effect on the Compton profile. The structures were obtained by using ab initio molecular dynamics simulations. Chosen radial distribution functions of the liquid structure are presented and found generally to be in good agreement with previous ab initio molecular dynamics and neutron scattering studies. The main contributions to the predicted difference Compton profile are found to arise from intermolecular changes in the phase transition. This prediction can be used for interpreting future experiments.
The electromagnetic coupling of helical modes, even those having different toroidal mode numbers, modifies the distribution of toroidal angular momentum in tokamak discharges. This can have deleterious effects on other transport channels as well as on magnetohydrodynamic (MHD) stability and disruptivity. At low levels of externally injected momentum, the coupling of core-localized modes initiates a chain of events, whereby flattening of the core rotation profile inside successive rational surfaces leads to the onset of a large m/n = 2/1 tearing mode and locked-mode disruption. With increased torque from neutral beam injection, neoclassical tearing modes in the core may phase-lock to each other without locking to external fields or structures that are stationary in the laboratory frame. The dynamic processes observed in these cases are in general agreement with theory, and detailed diagnosis allows for momentum transport analysis to be performed, revealing a significant torque density that peaks near the 2/1 rational surface. However, as the coupled rational surfaces are brought closer together by reducing q{sub 95}, additional momentum transport in excess of that required to attain a phase-locked state is sometimes observed. Rather than maintaining zero differential rotation (as is predicted to be dynamically stable by single-fluid, resistive MHD theory), these discharges develop hollow toroidal plasma fluid rotation profiles with reversed plasma flow shear in the region between the m/n = 3/2 and 2/1 islands. The additional forces expressed in this state are not readily accounted for, and therefore, analysis of these data highlights the impact of mode coupling on torque balance and the challenges associated with predicting the rotation dynamics of a fusion reactor—a key issue for ITER.
Separation of size and strain effects on diffraction line profiles has been studied in a round robin involving laboratory instruments and synchrotron radiation beamlines operating with different radiation, optics, detectors and experimental configurations. The studied sample, an extensively ball milled iron alloy powder, provides an ideal test case, as domain size broadening and strain broadening are of comparable size. The high energy available at some synchrotron radiation beamlines provides the best conditions for an accurate analysis of the line profiles, as the size–strain separation clearly benefits from a large number of Bragg peaks in the pattern; high counts, reliable intensity values in low-absorption conditions, smooth background and data collection at different temperatures also support the possibility to include diffuse scattering in the analysis, for the most reliable assessment of the line broadening effect. However, results of the round robin show that good quality information on domain size distribution and microstrain can also be obtained using standard laboratory equipment, even when patterns include relatively few Bragg peaks, provided that the data are of good quality in terms of high counts and low and smooth background.
In this study, a method of producing velocity profile maps from electrical capacitance volume tomography (ECVT) measurements by reconstructing displacement from measured changes in capacitance is developed and applied to fluidized bed systems. The mapping of the reconstruction leverages the gradient of the sensitivity distribution of the ECVT sensor to circumvent the need for image cross correlation techniques. Experimental data of both bubbling and slugging fluidized beds are collected in a cold flow model. Adaptation of the technique is discussed in detail, and velocity profiles are obtained for a range of gas flow rates. The produced velocity maps are compared against the established methods of cross correlation and against empirical correlations from the literature and are found to agree well in tracking slug and bubble velocity. The exception is when the tracked object is large relative to the ECVT sensor dimensions, a scenario that can be avoided through proper sensor design. The quantities of average velocity, momentum, and solid and gas volume fraction are derived from the image and velocity profiles. The results demonstrate and extend the power of ECVT as a measurement tool for the study and monitoring of gas–solid fluidized beds by providing a computationally cheaper alternative to 3-D cross correlation for deriving velocity profiles.
Purpose: Iterative image reconstruction gains more and more interest in clinical routine, as it promises to reduce image noise (and thereby patient dose), to reduce artifacts, or to improve spatial resolution. However, among vendors and researchers, there is no consensus of how to best achieve these goals. The authors are focusing on the aspect of geometric ray profile modeling, which is realized by some algorithms, while others model the ray as a straight line. The authors incorporate ray-modeling (RM) in nonregularized iterative reconstruction. That means, instead of using one simple single needle beam to represent the x-ray, the authors evaluate the double integral of attenuation path length over the finite source distribution and the finite detector element size in the numerical forward projection. Our investigations aim at analyzing the resolution recovery (RR) effects of RM. Resolution recovery means that frequencies can be recovered beyond the resolution limit of the imaging system. In order to evaluate, whether clinical CT images can benefit from modeling the geometrical properties of each x-ray, the authors performed a 2D simulation study of a clinical CT fan-beam geometry that includes the precise modeling of these geometrical properties. Methods: All simulations and reconstructions are performed in native fan-beam geometry. A water phantom with resolution bar patterns and a Forbild thorax phantom with circular resolution patterns representing calcifications in the heart region are simulated. An FBP reconstruction with a Ram–Lak kernel is used as a reference reconstruction. The FBP is compared to iterative reconstruction techniques with and without RM: An ordered subsets convex (OSC) algorithm without any RM (OSC), an OSC where the forward projection is modeled concerning the finite focal spot and detector size (OSC-RM) and an OSC with RM and with a matched forward and backprojection pair (OSC-T-RM, T for transpose). In all cases, noise was matched to be able to focus on comparing spatial resolution. The authors use two different simulation settings. Both are based on the geometry of a typical clinical CT system (0.7 mm detector element size at isocenter, 1024 projections per rotation). Setting one has an exaggerated source width of 5.0 mm. Setting two has a realistically small source width of 0.5 mm. The authors also investigate the transition from setting one to two. To quantify image quality, the authors analyze line profiles through the resolution patterns to define a contrast factor (CF) for contrast-resolution plots, and the authors compare the normalized cross-correlation (NCC) with respect to the ground truth of the circular resolution patterns. To independently analyze whether RM is of advantage, the authors implemented several iterative reconstruction algorithms: The statistical iterative reconstruction algorithm OSC, the ordered subsets simultaneous algebraic reconstruction technique (OSSART) and another statistical iterative reconstruction algorithm, denoted with ordered subsets maximum likelihood (OSML) algorithm. All algorithms were implemented both without RM (denoted as OSC, OSSART, and OSML) and with RM (denoted as OSC-RM, OSSART-RM, and OSML-RM). Results: For the unrealistic case of a 5.0 mm focal spot the CF can be improved by a factor of two due to RM: the 4.2 LP/cm bar pattern, which is the first bar pattern that cannot be resolved without RM, can be easily resolved with RM. For the realistic case of a 0.5 mm focus, all results show approximately the same CF. The NCC shows no significant dependency on RM when the source width is smaller than 2.0 mm (as in clinical CT). From 2.0 mm to 5.0 mm focal spot size increasing improvements can be observed with RM. Conclusions: Geometric RM in iterative reconstruction helps improving spatial resolution, if the ray cross-section is significantly larger than the ray sampling distance. In clinical CT, however, the ray is not much thicker than the distance between neighboring ray centers, as the focal spot size is small and detector crosstalk is negligible, due to reflective coatings between detector elements. Therefore,RM appears not to be necessary in clinical CT to achieve resolution recovery.
This study describes the characteristics of large-scale vertical velocity, apparent heating source ( Q 1 ) and apparent moisture sink ( Q 2 ) profiles associated with seasonal and diurnal variations of convective systems observed during the two intensive operational periods (IOPs) that were conducted from 15 February to 26 March 2014 (wet season) and from 1 September to 10 October 2014 (dry season) near Manaus, Brazil, during the Green Ocean Amazon (GoAmazon2014/5) experiment. The derived large-scale fields have large diurnal variations according to convective activity in the GoAmazon region and the morning profiles show distinct differences between the dry and wet seasons. In the wet season, propagating convective systems originating far from the GoAmazon region are often seen in the early morning, while in the dry season they are rarely observed. Afternoon convective systems due to solar heating are frequently seen in both seasons. Accordingly, in the morning, there is strong upward motion and associated heating and drying throughout the entire troposphere in the wet season, which is limited to lower levels in the dry season. In the afternoon, both seasons exhibit weak heating and strong moistening in the boundary layer related to the vertical convergence of eddy fluxes. A set of case studies of three typical types of convective systems occurring in Amazonia – i.e., locally occurring systems, coastal-occurring systems and basin-occurring systems – is also conducted to investigate the variability of the large-scale environment with different types of convective systems.
The Rubin LSSTComCam on-sky campaign performed at the end of 2024 provided observations of the Abell 360 galaxy cluster; these data allow a preliminary study of cluster weak lensing analysis using Rubin Data Preview 1 (DP1) data. Among all the steps required for such analyses, accurate modeling of the PSF is essential. This work uses several diagnostics, mostly based on the residuals between the second moments of stars and the PSF model, to characterize the accuracy of the PSF modeling in the A360 field. We find the level of the residuals to be sufficiently low not to hinder the measurement of the tangential shear profile around A360. With a simple source selection process, we demonstrate that outputs of the LSST Science Pipelines can be used to detect the tangential shear profile in Abell 360 at the 3.6σ level, and our analysis indicates that contamination from PSF modeling systematics is negligible.
Low surface brightness photometry of massive galaxies can probe the extended stellar halos and intracluster light but is often limited by background subtraction and masking systematics. Here we present radial surface brightness profiles for the brightest cluster galaxy of Abell 360 and 2dFGRS TGS323Z113 using Rubin Observatory Commissioning Camera (LSSTComCam) Data Preview 1 in the g, r, and z bands. Our results show that the LSSTComCam data can go down to ~28 mag arcsec^-2 in the r band. Comparison to DECaLS measurements processed with Legacyhalos shows good agreement of surface brightness profiles at small radii while the LSSTComCam data extend deeper at larger radii.
The Ozone Mapping and Profiler Suite Limb Profiler (OMPS/LP) was launched on board of the Soumi NPP space platform in late October 2011. It provides ozone-profiling capability with high-vertical resolution from 60 Ian to cloud top. In this study, an end-to-end Observing System Simulation Experiment (OSSE) of OMPS/LP ozone is discussed. The OSSE was developed at NASA's Global Modeling and Assimilation Office (GMAO) using the Goddard Earth Observing System (GEOS-5) data assimilation system. The "truth" for this OSSE is built by assimilating MLS profiles and OMI ozone columns, which is known to produce realistic three-dimensional ozone fields in the stratosphere and upper troposphere. OMPS/LP radiances were computed at tangent points computed by an appropriate orbital model. The OMPS/LP forward RT model, Instrument Models (IMs) and EDR retrieval model were introduced and pseudo-observations derived. The resultant synthetic OMPS/LP observations were evaluated against the "truth" and subsequently these observations were assimilated into GEOS-5. Comparison of this assimilated dataset with the "truth" enables comparisons of the likely uncertainties in 3-D analyses of OMPS/LP data. This study demonstrated the assimilation capabilities of OMPS/LP ozone in GEOS-5, with the monthly, zonal mean (O-A) smaller than 0.02ppmv at all levels, the nns(O-A) close to O.lppmv from 100hPa to 0.2hPa; and the mean(O-B) around the 0.02ppmv for all levels. The monthly zonal mean analysis generally agrees to within 2% of the truth, with larger differences of 2-4% (0.1-0.2ppmv) around 10hPa close to North Pole and in the tropical tropopause region, where the difference is above 20% due to the very low ozone concentrations. These OSSEs demonstrated that, within a single data assimilation system and the assumption that assimilated MLS observations provide a true rendition of the stratosphere, the OMPS/LP ozone data are likely to produce accurate analyses through much of the stratosphere. Additional studies are needed to evaluate the importance of the extremely high vertical resolution of the OMPS/LP ozone data
This report summarizes research performed in support of the NASA Glenn Research Center (GRC) Low-Pressure Turbine (LPT) Flow Physics Program. The work was performed experimentally at the U.S. Naval Academy faculties. The geometry corresponded to "Pak B" LPT airfoil. The test section simulated LPT flow in a passage. Three experimental studies were performed: (a) Boundary layer measurements for ten baseline cases under high and low freestream turbulence conditions at five Reynolds numbers of 25,000, 50,000, 100,000, 200,000, and 300,000, based on passage exit velocity and suction surface wetted length; (b) Passive flow control studies with three thicknesses of two-dimensional bars, and two heights of three-dimensional circular cylinders with different spanwise separations, at same flow conditions as the 10 baseline cases; (c) Active flow control with oscillating synthetic (zero net mass flow) vortex generator jets, for one case with low freestream turbulence and a low Reynolds number of 25,000. The Passive flow control was successful at controlling the separation problem at low Reynolds numbers, with varying degrees of success from case to case and varying levels of impact at higher Reynolds numbers. The active flow control successfully eliminated the large separation problem for the low Reynolds number case. Very detailed data was acquired using hot-wire anemometry, including single and two velocity components, integral boundary layer quantities, turbulence statistics and spectra, turbulent shear stresses and their spectra, and intermittency, documenting transition, separation and reattachment. Models were constructed to correlate the results. The report includes a summary of the work performed and reprints of the publications describing the various studies.This report summarizes research performed in support of the NASA Glenn Research Center (GRC) Low-Pressure Turbine (LPT) Flow Physics Program. The work was performed experimentally at the U.S. Naval Academy faculties. The geometry corresponded to "Pak B" LPT airfoil. The test section simulated LPT flow in a passage. Three experimental studies were performed: (a) Boundary layer measurements for ten baseline cases under high and low freestream turbulence conditions at five Reynolds numbers of 25,000, 50,000, 100,000, 200,000, and 300,000, based on passage exit velocity and suction surface wetted length; (b) Passive flow control studies with three thicknesses of two-dimensional bars, and two heights of three-dimensional circular cylinders with different spanwise separations, at same flow conditions as the 10 baseline cases; (c) Active flow control with oscillating synthetic (zero net mass flow) vortex generator jets, for one case with low freestream turbulence and a low Reynolds number of 25,000. The Passive flow control was successful at controlling the separation problem at low Reynolds numbers, with varying degrees of success from case to case and varying levels of impact at higher Reynolds numbers. The active flow control successfully eliminated the large separation problem for the low Reynolds number case. Very detailed data was acquired using hot-wire anemometry, including single and two velocity components, integral boundary layer quantities, turbulence statistics and spectra, turbulent shear stresses and their spectra, and intermittency, documenting transition, separation and reattachment. Models were constructed to correlate the results. The report includes a summary of the work performed and reprints of the publications describing the various studies. The folders in this supplement contain processed data in ASCII format. Streamwise pressure profiles and velocity profiles are included. The velocity profiles were acquired using single sensor and cross sensor hot-wire probes which were traversed from the wall to the freestream at various streamwise locations. In some of the flow control cases (3D Trips and Jets) profiles were acquired at multiple spanwise locations.
Foliage profiles retrieved froma scanning, terrestrial, near-infrared (1064 nm), full-waveformlidar, the Echidna Validation Instrument (EVI), agree well with those obtained from an airborne, near-infrared, full-waveform, large footprint lidar, the Lidar Vegetation Imaging Sensor (LVIS). We conducted trials at 5 plots within a conifer stand at Sierra National Forest in August, 2008. Foliage profiles retrieved from these two lidar systems are closely correlated (e.g., r = 0.987 at 100 mhorizontal distances) at large spatial coverage while they differ significantly at small spatial coverage, indicating the apparent scanning perspective effect on foliage profile retrievals. Alsowe noted the obvious effects of local topography on foliage profile retrievals, particularly on the topmost height retrievals. With a fine spatial resolution and a small beam size, terrestrial lidar systems complement the strengths of the airborne lidars by making a detailed characterization of the crowns from a small field site, and thereby serving as a validation tool and providing localized tuning information for future airborne and spaceborne lidar missions.
Since July of 2009, The Compact Reconnaissance Imaging Spectral Mapper (CRISM) onboard the Mars Re- connaissance Orbiter (MRO) has periodically obtained pole-to-pole observations (i.e., full MRO orbits) of limb scanned visible/near IR spectra (lambda= 0.4 - 4.0 micrometers, delta lambda approx. 10 nm- Murchie et al., 2007). These CRISM limb observations support the first seasonally and spatially extensive set of Mars 1.27 micometers O2 (1 delta(sub g)) day-glow profile retrievals (approx. 1100) over greater than or equal to 8-80 km altitudes. Their comparison to Laboratoire de Meteorologie Dynamique (LMD) global climate model (GCM) simulated O2 (1 delta(sub g)) volume emission rate (VER) profiles, as a function of altitude, latitude, and season (solar longitude, L(sub s), supports several key conclusions regarding Mars atmospheric water vapor (which is derived from O2 (1 delta(sub g)) emission rates), Mars O3, and the collisional de-excitation of O2 (1 delta(sub g)) in the Mars CO2 atmosphere. Current (Navarro et al., 2014) LMDGCM simulations of Mars atmospheric water vapor fall 2-3 times below CRISM derived water vapor abundances at 20-40 km altitudes over low-to-mid latitudes in northern spring (L(sub s) = 30-60 deg), and northern mid-to-high latitudes over northern summer (L(sub s) = 60-140 deg). In contrast, LMDGCM simulated water vapor is 2-5 times greater than CRISM derived values at all latitudes and seasons above 40 km, within the aphelion cloud belt (ACB), and over high-southern to mid-southern latitudes in southern summer (L(sub s) = 190-340 deg) at 15-35 km altitudes. Overall, the solstitial summer-to-winter hemisphere gradients in water vapor are reversed between the LMDGCM modeled versus the CRISM derived water vapor abundances above 10-30 km altitudes. LMDGCM-CRISM differences in water vapor profiles correlate with LMDGCM-CRISM differences in cloud mixing profiles; and likely reflect limitations in simulating cloud microphysics and radiative forcing, both of which restrict meridional transport of water from summer- to-winter hemispheres on Mars (Clancy et al., 1996; Montmessin et al., 2004; Steele et al., 2014; Navarro et al., 2014) and depend on uncertain cloud microphysical properties (Navarro et al., 2014). The derived low-to-mid latitude changes in Mars water vapor vertical distributions should reduce current model- data disagreements in column O3 and H2O2 abundances over low-to-mid latitudes (e.g., within the ACB; Lefevre et al., 2008; Encrenaz et al., 2015; Clancy et al., 2016). Lastly, the global/seasonal average com- parison of CRISM and LMDGCM O2 (1 delta(sub g)) VER below 20 km altitudes indicates a factor of approx. 3 times lower value (0.25 x 10(exp -20) cu cm sec(exp -1)) for the CO2 collisional de-excitation rate coefficient of O2 (1 delta(sub g)) than derived recently by Guslyakova et al. (2016).
Abstract Observations of thermodynamic and kinematic parameters associated with derivatives of the thermodynamics and wind fields, namely, advection, vorticity, divergence, and deformation, can be obtained by applying Green’s theorem to a network of observing sites. The five nodes that comprise the Atmospheric Radiation Measurement (ARM) Southern Great Plains (SGP) profiling network, spaced 50–80 km apart, are used to obtain measurements of these parameters over a finite region. To demonstrate the applicability of this technique at this location, it is first applied to gridded model output from the High-Resolution Rapid Refresh (HRRR) numerical weather prediction model, using profiles from the locations of ARM network sites, so that values calculated from this method can be directly compared to finite difference calculations. Good agreement is found between both approaches as well as between the model and values calculated from the observations. Uncertainties for the observations are obtained via a Monte Carlo process in which the profiles are randomly perturbed in accordance with their known error characteristics. The existing size of the ARM network is well suited to capturing these parameters, with strong correlations to model values and smaller uncertainties than a more closely spaced network, yet it is small enough that it avoids the tendency for advection to go to zero over a large area.
We quantify the accuracy of the temperature profiling from ground-based spectral infrared radiance observations at the American WAKE ExperimeNt (AWAKEN). Results from pre-campaign tests and comparisons with in-situ ground-based and airborne sensors at AWAKEN indicate that temperature profiles agree satisfactorily with traditional instruments for wind energy applications. The bias is within a fraction of a degree and appears to be related to atmospheric stability. Root-mean-square differences from the reference instruments are always smaller than a degree and are often well described by the online uncertainty estimation product. Height-to-height and site-to-site temperature differences are in excellent agreement with in-situ observations, which justifies the use of temperature profilers to characterize static stability and spatial gradients of temperature.