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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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NASA NTRS1977

The asteroid albedo scale. I - Laboratory polarimetry of meteorites

Laboratory reflection polarimetry is presented for crushed rocks, lunar soils, and 24 meteoritic samples. The polarization-phase curves found for the meteorites closely resemble telescopic results for minor planets, but only if the samples are crushed finely enough to coat the larger particles with dust. The results generally confirm the identifications previously made from reflection spectroscopy for the meteoritic analogues of various types of asteroids; the more metal-rich ordinary chondrites, for example, match telescopic observations of the S-type minor planets well enough, as do the basaltic achondrites for Vesta. No meteorite measured, however, has a geometric albedo less than 0.05 as required for many of the C asteroids. A calibration of the polarimetric slope-albedo law, now determined strictly for meteoritic samples, confirms the asteroid albedo and diameter scale derived by the independent method of thermal radiometry.

Zellner, B.↗
NASA NTRS1977

Optimization of confinement in a toroidal plasma subject to strong radial electric fields

A preliminary report on the identification and optimization of independent variables which affect the ion density and confinement time in a bumpy torus plasma is presented. The independent variables include the polarity, position, and number of the midplane electrode rings, the method of gas injection, and the polarity and strength of a weak vertical magnetic field. Some characteristic data taken under condition when most of the independent variables were optimized are presented. The highest value of the electron number density on the plasma axis is 3.2 x 10 to the 12th power/cc, the highest ion heating efficiency is 47 percent, and the longest particle containment time is 2.0 milliseconds.

Roth, J. R.↗
NASA NTRS1982

Chemistry of cometary meteoroids from video-tape records of meteor spectra

The chemistry of the cometary meteoroids was studied by closed circuit television observing systems. Vidicon cameras produce basic data on standard video tape and enable the recording of the spectra of faint shower meteors, consequently the chemical study is extended to smaller particles and we have a larger data bank than is available from the more conventional method of recording meteor spectra by photography. The two main problems in using video tape meteor spectrum records are: (1) the video tape recording has a much lower resolution than the photographic technique; (2) video tape is relatively new type of data storage in astronomy and the methods of quantitative photometry have not yet been fully developed in the various fields where video tape is used. The use of the most detailed photographic meteor spectra to calibrate the video tape records and to make positive identification of the more prominent chemical elements appearing in the spectra may solve the low resolution problem. Progress in the development of standard photometric techniques for the analysis of video tape records of meteor spectra is reported.

Millman, P. M.↗
NASA NTRS1994

Comet coma sample return instrument

The sample collection technology and instrument concept for the Sample of Comet Coma Earth Return Mission (SOCCER) are described. The scientific goals of this Flyby Sample Return are to return to coma dust and volatile samples from a known comet source, which will permit accurate elemental and isotopic measurements for thousands of individual solid particles and volatiles, detailed analysis of the dust structure, morphology, and mineralogy of the intact samples, and identification of the biogenic elements or compounds in the solid and volatile samples. Having these intact samples, morphologic, petrographic, and phase structural features can be determined. Information on dust particle size, shape, and density can be ascertained by analyzing penetration holes and tracks in the capture medium. Time and spatial data of dust capture will provide understanding of the flux dynamics of the coma and the jets. Additional information will include the identification of cosmic ray tracks in the cometary grains, which can provide a particle's process history and perhaps even the age of the comet. The measurements will be made with the same equipment used for studying micrometeorites for decades past; hence, the results can be directly compared without extrapolation or modification. The data will provide a powerful and direct technique for comparing the cometary samples with all known types of meteorites and interplanetary dust. This sample collection system will provide the first sample return from a specifically identified primitive body and will allow, for the first time, a direct method of matching meteoritic materials captured on Earth with known parent bodies.

Albee, A. L.↗
NASA NTRS1974

The variation of solar proton energy spectra size distribution with heliolongitude

A statistical study of the initial phases of 185 solar particle events was carried out using the data from cosmic ray experiments on IMP 4 and IMP 5. Special emphasis was placed on the identification of the associated solar flare, as the parent flare can be determined for 68% of the events. It appears probable that most of the unidentified increases occur on the non-visible disc of the sun. The existence of a 'preferred-connection' longitude between 20 W and 80 W was established by examining the heliolongitude of all the flare associated events. It is demonstrated that the energy spectra determined at the time of maximum particle in the 20 to 80 MeV or 4 to 20 Mev interval range give results identical to that obtained by the 'distance-travelled' method.

Vanhollebeke, M. A. I.↗
NASA NTRSDate not supplied

Biological Space Radiation Countermeasures to Enable Long Duration Exploration Missions

NASA’s career radiation limit for astronauts is 600 mSv. Currently planned missions beyond low Earth orbit (LEO) will expose crew to at least double that amount of radiation (for Mars missions). Therefore, to enable long duration exploration, countermeasures need to be deployed to reduce the long-term health outcomes of space radiation exposure. Limitations to the ability of spacecraft to shield against the high energy charged particles of the space radiation environment necessitate alternative methods to reduce overall space radiation risk for carcinogenesis. Recent successes in the arenas of Acute Radiation Syndrome (ARS) and clinical radiotherapy have demonstrated the efficacy of compound-based/biologicals in reducing the detrimental long term health outcomes associated with space radiation exposure. In recent years, the Space Radiation Element has funded the investigation of several such compounds including Avasopasem Manganese, CDDO-Me, Metformin, and γ-tocotrienol. The demonstrated efficacy of these compounds in reducing carcinogenesis, central nervous system, and cardiovascular disease risks demonstrate the need for, and potential benefit of, a robust countermeasure identification and development program with an initial starting suite of compounds available to validate others. The authors would also like to present highlights of a recent Space Radiation Element sponsored issue of Life Sciences in Space Research entitled “Breaking the Limit” on this specific topic.

Space Radiation↗
NASA NTRSDate not supplied

Biological Space Radiation Countermeasures to Enable Long Duration Exploration Missions

NASA’s career radiation limit for astronauts is 600 mSv. Currently planned missions beyond low Earth orbit (LEO) will expose crew to at least double that amount of radiation (for Mars missions). Therefore, to enable long duration exploration, countermeasures need to be deployed to reduce the long-term health outcomes of space radiation exposure. Limitations to the ability of spacecraft to shield against the high energy charged particles of the space radiation environment necessitate alternative methods to reduce overall space radiation risk for carcinogenesis. Recent successes in the arenas of Acute Radiation Syndrome (ARS) and clinical radiotherapy have demonstrated the efficacy of compound-based/biologicals in reducing the detrimental long term health outcomes associated with space radiation exposure. In recent years, the Space Radiation Element has funded the investigation of several such compounds including Avasopasem Manganese, CDDO-Me, Metformin, and γ-tocotrienol. The demonstrated efficacy of these compounds in reducing carcinogenesis, central nervous system, and cardiovascular disease risks demonstrate the need for, and potential benefit of, a robust countermeasure identification and development program with an initial starting suite of compounds available to validate others. The authors would also like to present highlights of a recent Space Radiation Element sponsored issue of Life Sciences in Space Research entitled “Breaking the Limit” on this specific topic.

Space Radiation↗
NASA NTRS1994

Nondestructive time-of-capture, location and velocity sensing in intact capture

Velocity sensing of hypervelocity particles was a significant field of interest from the 1960s to 1970s. Generically, velocity sensing has been approached by either of two methods: first, detecting direct plasma or light emissions onto a solid surface, or second, performing time-of-flight by film penetration, or by detecting charged particles passing a static electric field. Impact plasma techniques required the destruction of the particles. Multiple-film penetrations were also destructive, especially for small particles. There is a need for an integrated, reliable, and simple location and velocity sensor compatible to our intact capture underdense medium without contributing additional damage to the captured particle. Since cometary dust, like cosmic dust, is essentially randomly distributed, knowing the time of the dust capture and the location of the capture on the collector permits the identification of the specific dust particle captured. Velocity information helps to determine the trajectory of the dust particle. Our discovery of a very suitable acoustic sensor and fruitful experimental results have led to the realization of a flight velocity sensor with intact capture underdense media.

Tsou, Peter↗
NASA NTRS2012

Application of Principal Component Analysis to NIR Spectra of Phyllosilicates: A Technique for Identifying Phyllosilicates on Mars

Orbital near-infrared (NIR) reflectance spectra of the martian surface from the OMEGA and CRISM instruments have identified a variety of phyllosilicates in Noachian terrains. The types of phyllosilicates present on Mars have important implications for the aqueous environments in which they formed, and, thus, for recognizing locales that may have been habitable. Current identifications of phyllosilicates from martian NIR data are based on the positions of spectral absorptions relative to laboratory data of well-characterized samples and from spectral ratios; however, some phyllosilicates can be difficult to distinguish from one another with these methods (i.e. illite vs. muscovite). Here we employ a multivariate statistical technique, principal component analysis (PCA), to differentiate between spectrally similar phyllosilicate minerals. PCA is commonly used in a variety of industries (pharmaceutical, agricultural, viticultural) to discriminate between samples. Previous work using PCA to analyze raw NIR reflectance data from mineral mixtures has shown that this is a viable technique for identifying mineral types, abundances, and particle sizes. Here, we evaluate PCA of second-derivative NIR reflectance data as a method for classifying phyllosilicates and test whether this method can be used to identify phyllosilicates on Mars.

Rampe, E. B.↗
NASA NTRS2016

Image Correlation Pattern Optimization for Micro-Scale In-Situ Strain Measurements

The accuracy and precision of digital image correlation (DIC) is a function of three primary ingredients: image acquisition, image analysis, and the subject of the image. Development of the first two (i.e. image acquisition techniques and image correlation algorithms) has led to widespread use of DIC; however, fewer developments have been focused on the third ingredient. Typically, subjects of DIC images are mechanical specimens with either a natural surface pattern or a pattern applied to the surface. Research in the area of DIC patterns has primarily been aimed at identifying which surface patterns are best suited for DIC, by comparing patterns to each other. Because the easiest and most widespread methods of applying patterns have a high degree of randomness associated with them (e.g., airbrush, spray paint, particle decoration, etc.), less effort has been spent on exact construction of ideal patterns. With the development of patterning techniques such as microstamping and lithography, patterns can be applied to a specimen pixel by pixel from a patterned image. In these cases, especially because the patterns are reused many times, an optimal pattern is sought such that error introduced into DIC from the pattern is minimized. DIC consists of tracking the motion of an array of nodes from a reference image to a deformed image. Every pixel in the images has an associated intensity (grayscale) value, with discretization depending on the bit depth of the image. Because individual pixel matching by intensity value yields a non-unique scale-dependent problem, subsets around each node are used for identification. A correlation criteria is used to find the best match of a particular subset of a reference image within a deformed image. The reader is referred to references for enumerations of typical correlation criteria. As illustrated by Schreier and Sutton and Lu and Cary systematic errors can be introduced by representing the underlying deformation with under-matched shape functions. An important implication, as discussed by Sutton et al., is that in the presence of highly localized deformations (e.g., crack fronts), error can be reduced by minimizing the subset size. In other words, smaller subsets allow the more accurate resolution of localized deformations. Contrarily, the choice of optimal subset size has been widely studied and a general consensus is that larger subsets with more information content are less prone to random error. Thus, an optimal subset size balances the systematic error from under matched deformations with random error from measurement noise. The alternative approach pursued in the current work is to choose a small subset size and optimize the information content within (i.e., optimizing an applied DIC pattern), rather than finding an optimal subset size. In the literature, many pattern quality metrics have been proposed, e.g., sum of square intensity gradient (SSSIG), mean subset fluctuation, gray level co-occurrence, autocorrelation-based metrics, and speckle-based metrics. The majority of these metrics were developed to quantify the quality of common pseudo-random patterns after they have been applied, and were not created with the intent of pattern generation. As such, it is found that none of the metrics examined in this study are fit to be the objective function of a pattern generation optimization. In some cases, such as with speckle-based metrics, application to pixel by pixel patterns is ill-conditioned and requires somewhat arbitrary extensions. In other cases, such as with the SSSIG, it is shown that trivial solutions exist for the optimum of the metric which are ill-suited for DIC (such as a checkerboard pattern). In the current work, a multi-metric optimization method is proposed whereby quality is viewed as a combination of individual quality metrics. Specifically, SSSIG and two auto-correlation metrics are used which have generally competitive objectives. Thus, each metric could be viewed as a constraint imposed upon the others, thereby precluding the achievement of their trivial solutions. In this way, optimization produces a pattern which balances the benefits of multiple quality metrics. The resulting pattern, along with randomly generated patterns, is subjected to numerical deformations and analyzed with DIC software. The optimal pattern is shown to outperform randomly generated patterns.

Bomarito, G. F.↗
NASA NTRS2007

Aqueous Alteration of Basalts: Earth, Moon, and Mars

The geologic processes responsible for aqueous alteration of basaltic materials on Mars are modeled beginning with our knowledge of analog processes on Earth, i.e., characterization of elemental and mineralogical compositions of terrestrial environments where the alteration and weathering pathways related to aqueous activity are better understood. A key ingredient to successful modeling of aqueous processes on Mars is identification of phases that have formed by those processes. The purpose of this paper is to describe what is known about the elemental and mineralogical composition of aqueous alteration products of basaltic materials on Mars and their implications for specific aqueous environments based upon our knowledge of terrestrial systems. Although aqueous alteration has not occurred on the Moon, it is crucial to understand the behaviors of basaltic materials exposed to aqueous environments in support of human exploration to the Moon over the next two decades. Several methods or indices have been used to evaluate the extent of basalt alteration/weathering based upon measurements made at Mars by the Mars Exploration Rover (MER) Moessbauer and Alpha Particle X-Ray Spectrometers. The Mineralogical Alteration Index (MAI) is based upon the percentage of total Fe (Fe(sub T)) present as Fe(3+) in alteration products (Morris et al., 2006). A second method is the evaluation of compositional trends to determine the extent to which elements have been removed from the host rock and the likely formation of secondary phases (Nesbitt and Young, 1992; Ming et al., 2007). Most of the basalts that have been altered by aqueous processes at the two MER landing sites in Gusev crater and on Meridiani Planum have not undergone extensive leaching in an open hydrolytic system with the exception of an outcrop in the Columbia Hills. The extent of aqueous alteration however ranges from relatively unaltered to pervasively altered materials. Several experimental studies have focused upon the aqueous alteration of lunar materials and simulants (e.g., Keller and Huang, 1971; Eick et al., 1996). Lunar basalts are void of water and highly reduced, hence, these materials are initially very reactive when exposed to water under oxidizing conditions.

Ming, Douglas W.↗
NASA NTRS2019

Serendipitous Geodesy from Bennu's Short-Lived Moonlets

The Origins, Spectral Interpretation, Resource Identification, and Security-Regolith Explorer (OSIRIS-REx; or OREx) spacecraft arrived at its target, near-Earth asteroid (101955) Bennu, on December 3, 2018. The OSIRIS-REx spacecraft has since collected a wealth of scientific information in order to select a suitable site for sampling. Shortly after insertion into orbit on December 31, 2018, particles were identified in starfield images taken by the navigation camera (NavCam 1). Several groups within the OSlRlS-REx team analyzed the particle data in an effort to better understand this newfound activity of Bennu and to investigate the potential sensitivity of the particles to Bennu's geophysical parameters. A number of particles were identified through automatic and manual methods in multiple images, which could be turned into short sequences of optical tracking observations. Here, we discuss the precision orbit determination (OD) effort focused on these particles at NASA GSFC, which involved members of the Independent Navigation Team (INT) in particular. The particle data are combined with other OSIRIS-REx tracking data (radiometric from OSN and optical landmark data) using the NASA GSFC GEODYN orbit determination and geodetic parameter estimation software. We present the results of our study, particularly those pertaining to the gravity field of Bennu. We describe the force modeling improvements made to GEODYN specifically for this work, e.g., with a raytracing-based modeling of solar radiation pressure. The short-lived, low-flying moonlets enable us to determine a gravity field model up to a relatively high degree and order: at least degree 6 without constraints, and up to degree 10 when applying Kaula-like regularization. We can backward- and forward-integrate the trajectory of these particles to the ejection and landing sites on Bennu. We assess the recovered field by its impact on the OSIRIS-REx trajectory reconstruction and prediction quality in the various mission phases (e.g., Orbital A, Detailed Survey, and Orbital B).

Mazarico, Erwan↗
NASA NTRS2019

Highly Accurate Quartic Force Field and Rovibrational Spectroscopic Constants for the Azirinyl Cation (c-C2NH2+) and Its Isomers

Azirinyl cation is an aromatic cyclic molecule that is isoelectronic with cyclopropenylidene, c-C3H2, and c-C3H3+. Cyclopropenylidene has been shown to be ubiquitous, existing in many different astrophysical environments. Given the similar chemistry between C and N, and the relative abundances between C and N in astrophysical environments, it is expected that there should be aromatic ringed molecules that incorporate N in the ring, but as yet, no such molecule has been identified. To address this issue, the present study uses high levels of electronic structure theory to compute a highly accurate quartic force field (QFF) for azirinyl cation and its two lowest lying isomers, the cyanomethyl and isocyanomethyl cations. The theoretical approach uses the singles and doubles coupled-cluster method that includes a perturbative correction for connected triple excitations, CCSD(T), together with extrapolation to the one-particle basis set limit and corrections for scalar relativity and core-correlation. The QFF is then used in a second order vibrational perturbation theory analysis (VPT2) to compute the fundamental vibrational frequencies and rovibrational spectroscopic constants for all three C2NH2+ isomers. The reliability of the VPT2 vibrational frequencies is tested by comparison to vibrational configuration interaction (VCI) calculations and excellent agreement is found between the two approaches. Fundamental vibrational frequencies and rovibrational spectroscopic constants for all singly substituted 13C, 15N, and D isotopologues are also reported. It is expected that the highly accurate spectroscopic data reported herein will be useful in the identification of these cations in high resolution experimental or astronomical observations.

Partha P Bera↗
NASA NTRS2013

Herschel Observations of the Debris Disc Around HIP 92043

Context: Typical debris discs are composed of particles ranging from several micron sized dust grains to kilometer-sized asteroidal bodies, and their infrared emission peaks at wavelengths 60-100 microns. Recent Herschel DUNES (Dust Around Nearby Stars) observations have identified several debris discs around nearby Sun-like stars (F, G and K spectral type) with significant excess emission only at 160 microns. Aims: We observed HIP 92043 (110 Her, HD 173667) at far-infrared and sub-millimetre wavelengths with Herschel PACS (Photodetector Array Camera and Spectrometer) and SPIRE (Spectral and Photometric Imaging Receiver).Identification of the presence of excess emission from HIP 92043 and the origin and physical properties of any excess was undertaken through analysis of its spectral energy distribution (SED) and the PACS images. Methods: The PACS and SPIRE images were produced using the HIPE (Herschel Interactive Processing Environment) photProject map maker routine. Fluxes were measured using aperture photometry. A stellar photosphere model was scaled to optical and near infrared photometry and subtracted from the far-infared and sub-mm fluxes to determine the presence of excess emission. Source radial profiles were fitted using a 2D Gaussian and compared to a PSF (Point Spread Function) model based on Herschel observations of alpha Boo to check for extended emission. Results: Clear excess emission from HIP 92043 was observed at 70 and 100 microns. Marginal excess was observed at 160 and 250 microns.Analysis of the images reveals that the source is extended at 160 microns. A fit to the source SED is inconsistent with a photosphere and single temperature black body. Conclusions: The excess emission from HIP 92043 is consistent with the presence of an unresolved circumstellar debris disc at 70and 100 microns, with low probability of background contamination. The extended 160 micron emission may be interpreted as an additional cold component to the debris disc or as the result of background contamination along the line of sight. The nature of the 160 micron excess cannot be determined absolutely from the available data, but we favour a debris disc interpretation, drawing parallels with previously identified cold disc sources in the DUNES sample.

debris discs↗
NASA NTRS2004

Vibrational Spectroscopy of Ions and Radicals Present in the Interstellar Medium and in Planetary Atmospheres: A Theoretical Study

Anharmonic vibrational frequencies and intensities are calculated for OH(H2O)n and H(H2O)n radicals (that form on icy particles of the interstellar medium), HCO radical (the main intermediate in the synthesis of organic molecules in space), NH2(-) and C2H(-) anions, H5(+) cation, and other systems relevant to interstellar chemistry. In addition to pure ions and radicals, their complexes with water are studied to assess the effects of water environment on infrared spectra. The calculations are performed using the correlation-corrected vibrational self-consistent field (CC-VSCF) method with ab initio potential surfaces at the MP2 and CCSD(T) levels. Fundamental, overtone, and combination excitations are computed. The results are in good agreement with available experimental data and provide reliable predictions for vibrational excitations not yet measured in laboratory experiments. The data should be useful for interpretation of astronomically observed spectra and identification of ions and radicals present in the interstellar medium and in planetary atmospheres.

Chaban, Galina M.↗
NASA NTRS2014

In-Situ and Remote-Sensing Data Fusion Using Machine Learning Techniques to Infer Urban and Fire Related Pollution Plumes

Airmass type characterization is key in understanding the relative contribution of various emission sources to atmospheric composition and air quality and can be useful in bottom-up model validation and emission inventories. However, classification of pollution plumes from space is often not trivial. Sub-orbital campaigns, such as SEAC4RS (Studies of Emissions, Atmospheric Composition, Clouds and Climate Coupling by Regional Surveys) give us a unique opportunity to study atmospheric composition in detail, by using a vast suite of in-situ instruments for the detection of trace gases and aerosols. These measurements allow identification of spatial and temporal atmospheric composition changes due to various pollution plumes resulting from urban, biogenic and smoke emissions. Nevertheless, to transfer the knowledge gathered from such campaigns into a global spatial and temporal context, there is a need to develop workflow that can be applicable to measurements from space. In this work we rely on sub-orbital in-situ and total column remote sensing measurements of various pollution plumes taken aboard the NASA DC-8 during 2013 SEAC4RS campaign, linking them through a neural-network (NN) algorithm to allow inference of pollution plume types by input of columnar aerosol and trace-gas measurements. In particular, we use the 4STAR (Spectrometer for Sky-Scanning, Sun-Tracking Atmospheric Research) airborne measurements of wavelength dependent aerosol optical depth (AOD), particle size proxies, O3, NO2 and water vapor to classify different pollution plumes. Our method relies on assigning a-priori ground-truth labeling to the various plumes, which include urban pollution, different fire types (i.e. forest and agriculture) and fire stage (i.e. fresh and aged) using cluster analysis of aerosol and trace-gases in-situ and auxiliary (e.g. trajectory) data and the training of a NN scheme to fit the best prediction parameters using 4STAR measurements as input. We explore our misclassification rates as related to our ground-truth labels, and with multi-layered pollution plume cases. The next step in our analysis is to optimize parameter selection for a scheme that can be applied to space-borne aerosol and trace-gas observation platforms such as OMI, and future geostationary satellites such as TEMPO and GEO-CAPE.

Neural-network↗
NASA NTRS2017

Introduction: Photons and Ground-Based

A Conference on Measurement Techniques for Solar and Space Physics was held on 20-24 April 2015 in Boulder, Colorado, at the National Center for Atmospheric Research Center Green Campus. The present volume collects together the conference papers for photons and ground-based categories. This gathering of over 200 scientists and instrumentalists was born out of the desire to collect in one place the latest experiment and instrument technologies required for advancement of scientific knowledge in the disciplines of solar and space physics. The two goals for this conference and the subsequent publication of its content are (a) to describe measurement techniques and technology development needed to advance high priority science in the fields of solar and space physics; and (b) to provide a survey or reference of techniques for in situ measurement and remote sensing of space plasmas. Towards this end, our goal has always been inspired by the two 1998 Geophysical Monographs (Nos. 102 and 103) entitled, "Measurement Techniques in Space Plasmas" (particles and fields) [Pfaff et al., 1998a, 1998b], which have served as a reference and resource for advanced students, engineers, and scientists who wish to learn the fundamentals of measurement techniques and technology in this field. Those monographs were the product of an American Geophysical Union Chapman Conference that took place in Santa Fe, NM, in 1995: "Measurement Techniques in Space Plasmas-What Works, What Doesn't." Two decades later, we believe that it is appropriate to revisit this subject, in light of recent advances in technology, research platforms, and analysis techniques. Moreover, we now include direct measurements of neutral gases in the upper atmosphere, optical imaging techniques, and remote observations in space and on the ground. Accordingly, the workshop was organized among four areas of measurement techniques: particles, fields, photons, and ground-based. This two-set volume is largely composed of the content of that workshop. Special attention is given to those techniques and technologies that demonstrate promise of significant advancement in measurements that will enable the highest priority science as described in the 2012 National Research Council Decadal Survey [Baker and Zurbuchen et al., 2013]. Additionally, a broad tutorial survey of the current technologies is provided to serve as reference material and as a basis from which advanced and innovative ideas can be discussed and pursued. Included are instrumentation and techniques to observe the solar environment from its interior to its outer atmosphere, the heliosphere out to the interstellar regions, in geospace, and other planetary magnetospheres and atmospheres. To make significant progress in priority science as expressed in the National Research Council solar and space physics decadal survey and recent NASA Heliophysics roadmaps, identification of enabling new measurement techniques and technologies to be developed is required. Also, it is valuable to the community and future scientists and engineers to have a complete survey of the techniques and technologies used by the practitioners of solar and space physics. As with the 1995 conference and subsequent 1998 publication, it is incumbent on the community to identify those measurements that are particularly challenging and still require new techniques to be identified and tested to enable the necessary accuracy and resolution of certain parameters to be achieved. The following is a partial list of the measurement technique categories that are featured in these special publications: Particles; Thermal plasma to MeV energetic particles, neutral gas properties including winds, density, temperature, and composition, and enhanced neutral atom imaging; Fields; DC electric and magnetic fields, plasma waves, and electron drift instruments from which the plasma velocity information provides a measure of the DC electric field; Photons; Instruments sensitive from the near-infrared to X-rays; Contributions of techniques and technology for optical design, optical components, sensors, material selection for cameras, telescopes, and spectrographs; Ground based; Remote sensing methods for solar and geospace activity and space weather. The focus includes solar observatories, all-sky cameras, lidars, and ionosphere thermosphere mesosphere observatory systems such as radars, ionosondes, GPS receivers, magnetometers, conjugate observations, and airborne campaigns. The present volume collects together the papers for photons and ground-based categories. The companion volume collects together the papers for particles and fields categories. It is recognized that there are measurement techniques that overlap among the four categories. For example, use of microchannel plate detectors is used in photon and particle measurement techniques or the observation of visible photons and magnetic fields in space and on the ground share common technologies. Therefore, the reader should consider the entire collection of papers as they seek to understand particular applications. We hope that these volumes will be as valuable as a reference for our community as the earlier 1998 volumes have been.

Spann, James↗
NASA NTRSDate not supplied

Microbial Monitoring of New Cleanrooms Used to Curate Astrobiologically Relevant Asteroid Samples from Bennu and Ryugu

Introduction: NASA has constructed two new cleanrooms to house materials from the OSRIS-REx and Hayabusa2 missions to the asteroids Ryugu (162173) and Bennu (101955), respectively. In accordance with standard astromaterials curation practices, these cleanrooms will be monitored for particulate contamination and maintained to ISO 5 equivalent standards1. Since the samples in these collections are expected to contain prebiotic organic compounds that may help explain the origin of life on Earth, these labs will also be monitored for organic and biological contamination2. Samples from Ryugu arrived on Earth in December, 2020. After basic characterization in Japan, NASA received a subset of these samples at the astromaterials curation facility in Houston in December of 2021. OSIRIS-REx is expected to return samples in September, 2023. Here we present preliminary microbial monitoring results from monthly monitoring of these new labs and the connected microtomy and staging areas that support them, as they are being commissioned. We also compare these results to baseline values for other astromaterials curation labs. We will also briefly describe additional cleaning efforts employed to reduce the bioburden in these new cleanrooms. Methods: Microbial samples were collected from surfaces using a dry macrofoam swab (Puritan Brand 2518051PFRNDFD). Swabs were also opened in the lab but not touched to any surfaces to function as negative controls. Samples and controls were processed inside a class II biosafety cabinet to avoid inadvertent cross contamination. The swabs were suspended in 15 ml of PBS (Phosphate Buffered Saline) and vortexed for 20 seconds to remove cells from the swab surface. The PBS was used to inoculate Petri dishes filled with TSA (Tryptic Soy Agar), Blood Agar, or Reasoners 2 agar to check for microbial growth. Each plate was inoculated with 0.1 ml of PBS. The TSA and blood agar plates were incubated at 35˚C and the Reasoners 2 agar plates were incubated at 25˚C for seven days. Petri dishes filled with Potato dextrose agar, Saboraud dextrose agar, or Saboraud dextrose agar with 0.1 mg/ml of chloramphenicol, an antibiotic, were used to check for fungal growth. These plates were inoculated with 0.3 ml of PBS and incubated at 30˚C. The remaining PBS was frozen at -80 ˚C for DNA sequencing. After incubation, isolates were counted and reisolated for identification. Isolates were identified using the VITEK23 system or by sequencing a portion of the 16S rRNA gene for bacteria or the ribosomal internal transcribed spacer (ITS) for fungi. Sequencing was performed with an ABI 3500 Sanger sequencer. Results: During our initial sampling, six of the seven sites sampled (86%) displayed bacterial or fungal growth. Samples collected from the staging areas and microtomy labs are not included in this calculation since those areas are maintained at a lower ISO 7 equivalent cleanliness standard. A month later, only three of the seven sites (43%) displayed bacterial growth. No fungal growth was detected in the second sampling. Since new equipment had been introduced to the Hayabusa2 lab since the first round of sampling, an additional three sampling sites were included in the second round of sampling. None of these sites displayed microbial growth. These sites will be included in all future sampling efforts. Bacterial isolates have been identified from the following genera at multiple time points: Micrococcus, Staphylococcus, and Bacillus. Isolates from the genera: Microbacterium, Nocardioides, Methylocystis, and Microvirga were identified in the initial sampling, but were not present at later time points. Identification of fungal isolates is in progress. Results are summarized in Table 1. Discussion: The recovery rate or percentage of positive samples4 was initially 86%, which is higher than the median recovery rate for comparable ISO 5 equivalent curation labs like Stardust (33%), Hayabusa (33%), and Cosmic Dust (50%). However, after a month of operation, the recovery rate for these same sites decreased to 43%, which is similar to what we observe in comparable curation cleanrooms with no microbial control requirements. Adding in the new sampling sites further decreases the recovery rate to 30%. With the reduction in recovery rate, we also observed a decrease in microbial diversity. At the first time point, we observed at least 10 different bacterial species and at least two different fungi. This is a higher diversity than the median values for comparable ISO 5 equivalent labs (2-4 isolates per sampling event). After the second sampling, we observed at least 4 bacterial species and no fungi, which is more consistent with comparable labs. We expect the recovery rate and diversity in both labs to continue to decrease as routine operation continues. We will use ultrapure hydrogen peroxide to disinfect equipment and work areas prior to opening any sample containers. Most of the bacterial and fungal isolates were detected on samples from the cleanroom floors. This is consistent with baseline results from other curation labs. Organisms from the genera Bacillus, Staphylococcus, and Micrococcus that were repeatedly detected are common in cleanrooms and on human skin5,6. These organisms are generally thought to be introduced when people enter the cleanroom. Microbacterium, Nocardioides, and Microvirga have also previously been identified in astromaterials cleanrooms, but not as frequently as Bacillus, Staphylococcus, and Micrococcus. Methylocystis is a novel genus in the astromaterials cleanrooms, but it was identified with low accuracy (93% match in the sequenced region of the 16S rRNA gene) and further work is needed to confirm this identification. Microbacterium is a diverse genus with isolates identified from terrestrial and aquatic sediments. Some species of Microbacterium are capable of degrading complex organic compounds found in crude oil. The presence of these bacteria in the OSIRIS REx and Hayabusa2 cleanrooms should be closely monitored. Methylocystis is a genus of methanotrophic bacteria capable of oxidizing methane. If this identification proves to be correct and it is detected again, it should be closely monitored as well. Under nominal operating conditions, samples should not ever encounter the cleanroom floor or other high traffic areas. If we observe an increase in the bioburden in sensitive work areas that appears to be influenced by organism transfer from high traffic areas like the floors, we can employ additional hydrogen peroxide treatments to disinfect high traffic areas. Routine microbial monitoring of these labs will ensure that NASA’s astromaterials collections remain pristine and useful for scientific study. Table 1. Sampling Locations and Colony Counts Bacterial CFUa Fungal CFU Bacterial CFU Fungal CFU Lab - Location 11/2/2021 11/2/2021 12/13/2021 12/13/2021 H2b-Floor 4 8 1 0 H2-staging pass through 3 0 0 0 H2-microtomy pass through TNTCc 0 0 0 H2 Microscope 1 NA NA 0 0 H2 Microscope 2 NA NA 0 0 H2-Table NA NA 0 0 OREXd- microtomy pass through 0 0 6 0 OREX – Anteroom pass through 0 0 0 0 OREX – Floor 1 2 0 0 OREX Witness Foil Table 3 0 1 0 Staging-Floor 16 0 15 0 Microtomy-Floor 3 0 2 0 a: CFU = Colony Forming Unit b: H2 = Hayabusa2 Lab c: TNTC = too numerous to count d: OREX = OSIRIS-REx Lab References: 1. ISO 14644-1:2015 - Cleanrooms and associated controlled environments -- Part 1: Classification of air cleanliness by particle concentration. 37 (2015). 2. McCubbin, F. M. et al. Space Sci Rev 215, (2019). 3. Pincus, D. H. Encyclopedia of Rapid Microbiological Methods (2005). 4. The United States Pharmacopeial Convention. USP General Chapter <1116> 17, 784–794 (2013). 5. Sheraba, N. S., Yassin, A. S. & Amin, M. BMC Research Notes 3, 278 (2010). 6. Utescher, C. L. de A., Franzolin, M. R., Trabulsi, L. R. & Gambale, V. Brazilian Journal of Microbiology 38, 710–716 (2007).

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