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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 73 records · Page 4
All sourcesNASA NTRSDOE OSTI
NASA NTRS1977

Transmission in nonuniform ducts - A comparative evaluation of finite element and weighted residuals computational schemes

The Method of Weighted Residuals (MWR) and the Finite Element Method (FEM) are considered as computational schemes in the problem of acoustic transmission in nonuniform ducts. MWR is presented in an improved form which includes the interaction of acoustic modes (irrotational) and hydrodynamic modes (rotational). FEM is based on a weighted residuals formulation using eight noded isoparametric elements. Both are applicable to two-dimensional and axially symmetric problems. Calculations are made for several sample problems to demonstrate accuracy and relative efficiency. One test case has implications in the phenomenon of subsonic acoustic choking and it is found that a large transmission loss is not an automatic consequence of propagation against a high subsonic mean flow.

Eversman, W.↗
NASA NTRS2012

Monte-Carlo Simulations of the Suzaku-XRS Residual Background Spectrum

Cryogenic micro-calorimeters are suitable to detect small amounts of energy deposited by electromagnetic and nuclear interactions, which makes them attractive in a variety of applications on ground and in space. The only X-ray microcalorimeter that operated in orbit to date is the X-Ray Spectrometer on-board of the Japanese Suzaku satellite. We discuss the analysis of the components of its residual background spectrum with the support of Monte-Carlo simulations.

X-ray↗
NASA NTRS1988

A unified process for systems identification based on performance assessment

Many modern spacecraft are complex multi-body dynamic systems where the bodies are connected by several active control systems for pointing and isolation. Mission requirements indicate that many structural modes and possibly some nonlinear effects will require characterization. Thus, even characterization at the subsystem level will become more difficult than usual. System level characterization difficulties will be compounded by the fact that only limited ground testing will be possible on the full up system, and flight testing will be restricted by an extremely limited measurements set. The object of the present discussion is the application of matrix majorant theory to the problem of assessing dynamic system performance when knowledge of the system is uncertain. We show how majorants provide an effective tool to relate required performance output to system identification test quality in terms of residual uncertainty in input-output relations, parameter values, nonlinearities, and interactions. The underlying machinery consists of the block-norm matrix which is a nonnegative matrix each of whose elements is the norm of a block of a suitably partitioned matrix. A matrix which bounds the block-norm matrix in the sense of nonnegative matrices, i.e., element by element is known as a majorant.

Hyland, C.↗
NASA NTRS2009

Computational Study on Full-length Human Ku70 with Double Stranded DNA: Dynamics, Interactions and Functional Implications

The Ku70/80 heterodimer is the first repair protein in the initial binding of double-strand break (DSB) ends following DNA damage, and is a component of nonhomologous end joining repair, the primary pathway for DSB repair in mammalian cells. In this study we constructed a full-length human Ku70 structure based on its crystal structure, and performed 20 ns conventional molecular dynamic (CMD) simulations on this protein and several other complexes with short DNA duplexes of different sequences. The trajectories of these simulations indicated that, without the topological support of Ku80, the residues in the bridge and C-terminal arm of Ku70 are more flexible than other experimentally identified domains. We studied the two missing loops in the crystal structure and predicted that they are also very flexible. Simulations revealed that they make an important contribution to the Ku70 interaction with DNA. Dislocation of the previously studied SAP domain was observed in several systems, implying its role in DNA binding. Targeted molecular dynamic (TMD) simulation was also performed for one system with a far-away 14bp DNA duplex. The TMD trajectory and energetic analysis disclosed detailed interactions of the DNA-binding residues during the DNA dislocation, and revealed a possible conformational transition for a DSB end when encountering Ku70 in solution. Compared to experimentally based analysis, this study identified more detailed interactions between DNA and Ku70. Free energy analysis indicated Ku70 alone is able to bind DNA with relatively high affinity, with consistent contributions from various domains of Ku70 in different systems. The functional implications of these domains in the processes of Ku heterodimerization and DNA damage recognition and repair can be characterized in detail based upon this analysis.

Hu, Shaowen↗
NASA NTRS2002

Isolation and characterization of a novel calmodulin-binding protein from potato

Tuberization in potato is controlled by hormonal and environmental signals. Ca(2+), an important intracellular messenger, and calmodulin (CaM), one of the primary Ca(2+) sensors, have been implicated in controlling diverse cellular processes in plants including tuberization. The regulation of cellular processes by CaM involves its interaction with other proteins. To understand the role of Ca(2+)/CaM in tuberization, we have screened an expression library prepared from developing tubers with biotinylated CaM. This screening resulted in isolation of a cDNA encoding a novel CaM-binding protein (potato calmodulin-binding protein (PCBP)). Ca(2+)-dependent binding of the cDNA-encoded protein to CaM is confirmed by (35)S-labeled CaM. The full-length cDNA is 5 kb long and encodes a protein of 1309 amino acids. The deduced amino acid sequence showed significant similarity with a hypothetical protein from another plant, Arabidopsis. However, no homologs of PCBP are found in nonplant systems, suggesting that it is likely to be specific to plants. Using truncated versions of the protein and a synthetic peptide in CaM binding assays we mapped the CaM-binding region to a 20-amino acid stretch (residues 1216-1237). The bacterially expressed protein containing the CaM-binding domain interacted with three CaM isoforms (CaM2, CaM4, and CaM6). PCBP is encoded by a single gene and is expressed differentially in the tissues tested. The expression of CaM, PCBP, and another CaM-binding protein is similar in different tissues and organs. The predicted protein contained seven putative nuclear localization signals and several strong PEST motifs. Fusion of the N-terminal region of the protein containing six of the seven nuclear localization signals to the reporter gene beta-glucuronidase targeted the reporter gene to the nucleus, suggesting a nuclear role for PCBP.

NASA Discipline Plant Biology↗
NASA NTRS1988

Automatic Scheduling and Planning (ASAP) in future ground control systems

This report describes two complementary approaches to the problem of space mission planning and scheduling. The first is an Expert System or Knowledge-Based System for automatically resolving most of the activity conflicts in a candidate plan. The second is an Interactive Graphics Decision Aid to assist the operator in manually resolving the residual conflicts which are beyond the scope of the Expert System. The two system designs are consistent with future ground control station activity requirements, support activity timing constraints, resource limits and activity priority guidelines.

Matlin, Sam↗
NASA NTRS1994

Three-dimensional structure of Schistosoma japonicum glutathione S-transferase fused with a six-amino acid conserved neutralizing epitope of gp41 from HIV

The 3-dimensional crystal structure of glutathione S-transferase (GST) of Schistosoma japonicum (Sj) fused with a conserved neutralizing epitope on gp41 (glycoprotein, 41 kDa) of human immunodeficiency virus type 1 (HIV-1) was determined at 2.5 A resolution. The structure of the 3-3 isozyme rat GST of the mu gene class was used as a molecular replacement model. The structure consists of a 4-stranded beta-sheet and 3 alpha-helices in domain 1 and 5 alpha-helices in domain 2. The space group of the Sj GST crystal is P4(sub 3)2(sub 1)2 with unit cell dimensions of a = b = 94.7 A, and c = 58.1 A. The crystal has 1 GST monomer per asymmetric unit, and 2 monomers that form an active dimer are related by crystallographic 2-fold symmetry. In the binding site, the ordered structure of reduced glutathione is observed. The gp41 peptide (Glu-Leu-Asp-Lys-Trp-Ala) fused to the C-terminus of Sj GST forms a loop stabilized by symmetry-related GSTs. The Sj GST structure is compared with previously determined GST structures of mammalian gene classes mu, alpha, and pi. Conserved amino acid residues among the 4 GSTs that are important for hydrophobic and hydrophilic interactions for dimer association and glutathione binding are discussed.

Lim, Kap↗
NASA NTRS1999

Investigation of the Influence of Microgravity on Transport Mechanisms in a Virtual Spaceflight Chamber: A Ground Based Program

In January 1992, the IML-1 FES experiment produced a set of classic experimental data and a 40 hour holographic "movie" of an ensemble of spheres in a fluid in microgravity. Because the data are in the form of holograms, we can study the three-dimensional distribution of particles with unprecedented detail by a variety of methods and for a wide variety of interests. The possession of the holographic movie is tantamount to having a complex experiment in space while working in an easily accessible laboratory on earth. The movie contains a vast amount of useful data, including residual g, g-jitter, convection and transport data, and particle fluid interaction data. The information content in the movie is so great that we have scarcely begun to tap into the data that is actually available in the more than 1000 holograms, each containing as much as 1000 megabytes of information. This ground-based project is exploiting this data and the concept of holographic storage of spaceflight data to provide an understanding of the effects of microgravity in materials processing. This paper provides the foundation, objectives, and status of the ground based project. The primary objective of this project is to advance the understanding of microgravity effects on crystal growth, convection in materials processing in the space environment, and complex transport phenomena at low Reynolds numbers. This objective is being achieved both experimentally and theoretically. Experiments are making use of existing holographic data recorded during the IML- I spaceflight. A parallel theoretical effort is providing the models for understanding the particle fields and their physics in the microgravity environment.

Trolinger, James D.↗
NASA NTRS1994

Three-dimensional structure of Schistosoma japonicum glutathione S-transferase fused with a six-amino acid conserved neutralizing epitope of gp41 from HIV

The 3-dimensional crystal structure of glutathione S-transferase (GST) of Schistosoma japonicum (Sj) fused with a conserved neutralizing epitope on gp41 (glycoprotein, 41 kDa) of human immunodeficiency virus type 1 (HIV-1) (Muster T et al., 1993, J Virol 67:6642-6647) was determined at 2.5 A resolution. The structure of the 3-3 isozyme rat GST of the mu gene class (Ji X, Zhang P, Armstrong RN, Gilliland GL, 1992, Biochemistry 31:10169-10184) was used as a molecular replacement model. The structure consists of a 4-stranded beta-sheet and 3 alpha-helices in domain 1 and 5 alpha-helices in domain 2. The space group of the Sj GST crystal is P4(3)2(1)2, with unit cell dimensions of a = b = 94.7 A, and c = 58.1 A. The crystal has 1 GST monomer per asymmetric unit, and 2 monomers that form an active dimer are related by crystallographic 2-fold symmetry. In the binding site, the ordered structure of reduced glutathione is observed. The gp41 peptide (Glu-Leu-Asp-Lys-Trp-Ala) fused to the C-terminus of Sj GST forms a loop stabilized by symmetry-related GSTs. The Sj GST structure is compared with previously determined GST structures of mammalian gene classes mu, alpha, and pi. Conserved amino acid residues among the 4 GSTs that are important for hydrophobic and hydrophilic interactions for dimer association and glutathione binding are discussed.

Schistosoma japonicum/enzymology/genetics/immunolo↗
NASA NTRS2018

In-Flight Calibration of Hitomi Soft X-Ray Spectrometer. (1) Background

The X-Ray Spectrometer (XRS) instrument of Suzaku provided the first measurement of the non-X-ray background (NXB) of an X-ray calorimeter spectrometer, but the data set was limited. The Soft X-ray Spectrometer (SXS) instrument of Hitomi was able to provide a more detailed picture of X-ray calorimeter background, with more than 360 ks of data while pointed at the Earth, and a comparable amount of blank-sky data. These data are important not only for analyzing SXS science data, but also for categorizing the contributions to the NXB in X-ray calorimeters as a class. In this paper, we present the contributions to the SXS NXB, the types and effectiveness of the screening, the interaction of the screening with the broad-band redistribution, and the residual background spectrum as a function of magnetic cut-off rigidity. The orbit-averaged SXS NXB in the range 0.3-12 keV was 4 × 10(exp −2) counts s(exp −1) -sq cm. This very low background in combination with groundbreaking spectral resolution gave SXS unprecedented sensitivity to weak spectral lines.

Kilbourne, Caroline A.↗
NASA NTRS1983

A mechanistic model of Eulerian, Lagrangian mean, and Lagrangian ozone transport by steady planetary waves

Ozone transport is calculated for steady, dissipative planetary waves using the Eulerian, Lagrangian mean, and residual circulation. A Lagrangian model of parcel dynamics is used to interpret planetary wave-photochemistry interaction. In chemically active regions the mean field ozone changes are found to be significant only where there are large gradients in chemical sources and sinks along parcel trajectories. The largest changes in the mean field are found in the lower stratosphere and are due to the Lagrangian mean advection. When the Lagrangian mean advection is approximated by the residual circulation, errors in the transport velocities as large as 30 pct may occur.

Rood, R. B.↗
NASA NTRS1997

Structure of an anti-HIV-1 hammerhead ribozyme complex with a 17-mer DNA substrate analog of HIV-1 gag RNA and a mechanism for the cleavage reaction: 750 MHz NMR and computer experiments

The structure of an anti-HIV-1 ribozyme-DNA abortive substrate complex was investigated by 750 MHz NMR and computer modeling experiments. The ribozyme was a chimeric molecule with 30 residues-18 DNA nucleotides, and 12 RNA residues in the conserved core. The DNA substrate analog had 17 residues. The chimeric ribozyme and the DNA substrate formed a shortened ribozyme-abortive substrate complex of 47 nucleotides with two DNA stems (stems I and III) and a loop consisting of the conserved core residues. Circular dichroism spectra showed that the DNA stems assume A-family conformation at the NMR concentration and a temperature of 15 degrees C, contrary to the conventional wisdom that DNA duplexes in aqueous solution populate entirely in the B-form. It is proposed that the A-family RNA residues at the core expand the A-family initiated at the core into the DNA stems because of the large free energy requirement for the formation of A/B junctions. Assignments of the base H8/H6 protons and H1' of the 47 residues were made by a NOESY walk. In addition to the methyl groups of all T's, the imino resonances of stems I and III and AH2's were assigned from appropriate NOESY walks. The extracted NMR data along with available crystallographic data, were used to derive a structural model of the complex. Stems I and III of the final model displayed a remarkable similarity to the A form of DNA; in stem III, a GC base pair was found to be moving into the floor of the minor groove defined by flanking AT pairs; data suggest the formation of a buckled rhombic structure with the adjacent pair; in addition, the base pair at the interface of stem III and the loop region displayed deformed geometry. The loop with the catalytic core, and the immediate region of the stems displayed conformational multiplicity within the NMR time scale. A catalytic mechanism for ribozyme action based on the derived structure, and consistent with biochemical data in the literature, is proposed. The complex between the anti HIV-1 gag ribozyme and its abortive DNA substrate manifests in the detection of a continuous track of A.T base pairs; this suggests that the interaction between the ribozyme and its DNA substrate is stronger than the one observed in the case of the free ribozyme where the bases in stem I and stem III regions interact strongly with the ribozyme core region (Sarma, R. H., et al. FEBS Letters 375, 317-23, 1995). The complex formation provides certain guidelines in the design of suitable therapeutic ribozymes. If the residues in the ribozyme stem regions interact with the conserved core, it may either prevent or interfere with the formation of a catalytically active tertiary structure.

NASA Discipline Exobiology↗
NASA NTRS1977

Ionospheric range-rate effects in satellite-to-satellite tracking

Investigation of ionospheric range and range-rate corrections in satellite-to-satellite tracking were investigated. Major problems were cited and the magnitude of errors that have to be considered for communications between satellites and related experiments was defined. The results point to the need of using a sophisticated modeling approach incorporating daily solar data, and where possible actual ionospheric measurements as update information, as a simple median model cannot possibly account for the complex interaction of the many variables. The findings provide a basis from which the residual errors can be estimated after ionospheric modeling is incorporated in the reduction. Simulations were performed for satellites at various heights: Apollo, Geos, and Nimbus tracked by ATS-6; and in two different geometric configurations: coplanar and perpendicular orbits.

Lipofsky, J. R.↗
NASA NTRS1997

Energetics of the lattice: packing elements in crystals of four-stranded intercalated cytosine-rich DNA molecules

Condensation of single molecules from solution into crystals represents a transition between distinct energetic states. In solution, the atomic interactions within the molecule dominate. In the crystalline state, however, a set of additional interactions are formed between molecules in close contact in the lattice--these are the packing interactions. The crystal structures of d(CCCT), d(TAACCC), d(CCCAAT), and d(AACCCC) have in common a four-stranded intercalated cytosine segment, built by stacked layers of cytosine.cytosine+ (C.C+) base pairs coming from two parallel duplexes that intercalate into each other with opposite polarity. The intercalated cytosine segments in these structures are similar in their geometry, even though the sequences crystallized in different space groups. In the crystals, adenine and thymine residues of the sequences are used to build the three-dimensional crystal lattice by elaborately interacting with symmetry-related molecules. The packing elements observed provide novel insight about the copious ways in which nucleic acid molecules can interact with each other--for example, when folded in more complicated higher order structures, such as mRNA and chromatin.

Non-NASA Center↗
NASA NTRS1999

Optical model methods of predicting nuclide production cross sections from heavy ion fragmentation

Quantum mechanical optical potential methods for calculating inclusive isotope and element production cross sections from the fragmenting of heavy nuclei by intermediate- and high-energy protons and heavy ions are presented based upon a modified abrasion-ablation-FSI (frictional spectator interaction) collision model. The abrasion stage is treated as a quantum mechanical knockout process that leaves the residual prefragment in an excited state. Prefragment excitation energies are estimated using a combined liquid drop and FSI method. In ablation the prefragment deexcites by particle and photon emission to produce the final fragment. Contributions from electromagnetic dissociation to single nucleon removal cross sections are incorporated using a Weiszacker-Williams theory that includes electric dipole and electric quadrupole interactions. Estimates of elemental and isotopic production cross sections are in good agreement with published cross section measurements for a variety of projectile-target-beam energy combinations.

NASA Discipline Radiation Health↗
NASA NTRS1980

Experiments on the amplification of optical activity

Chemical mechanisms for the amplification of small, abiotically produced enantiomeric excesses leading to the complete stereo specificity of all biochemical reactions observed in the present-day biosphere are investigated quantitatively. Partial copolymerization of a mixture of R- and S-leucine or R- and S-valine N-carboxy anhydrides containing a known excess of one enantiomer was induced and the enantiomeric composition of the resulting oligomer was analyzed by gas chromatography. It is found that the 50% polymerization of leucine mixtures having excesses of 8 to 70% of either enantiomer leads to a significant enhancement of the enantiomeric excess of the polymer, accompanied by a corresponding decrease in the enantiomeric excess of the unpolymerized residue. On the other hand, 25-50% polymerization of valine mixtures is observed to result in polymers showing a decreased enantiomeric excess relative to the starting mixture and corresponding increases in those of the residue. Results of the leucine polymerization are interpreted as supporting the theory of steric interactions between the monomer and the helical structure of the polymer leading to the enrichment of one enantiomer, and possible mechanisms for the reverse stereospecificity observed in valine are discussed.

Blair, N. E.↗
NASA NTRS2012

Damage Tolerance Assessment of Friction Pull Plug Welds

Friction stir welding is a solid state welding process developed and patented by The Welding Institute in Cambridge, England. Friction stir welding has been implemented in the aerospace industry in the fabrication of longitudinal welds in pressurized cryogenic propellant tanks. As the industry looks to implement friction stir welding in circumferential welds in pressurized cryogenic propellant tanks, techniques to close out the termination hole associated with retracting the pin tool are being evaluated. Friction pull plug welding is under development as a one means of closing out the termination hole. A friction pull plug weld placed in a friction stir weld results in a non-homogenous weld joint where the initial weld, plug weld, their respective heat affected zones and the base metal all interact. The welded joint is a composite, plastically deformed material system with a complex residual stress field. In order to address damage tolerance concerns associated with friction plug welds in safety critical structures, such as propellant tanks, nondestructive inspection and proof testing may be required to screen hardware for mission critical defects. The efficacy of the nondestructive evaluation or the proof test is based on an assessment of the critical flaw size in the test or service environments. Test data relating residual strength capability to flaw size in two aluminum alloy friction plug weld configurations is presented.

McGill, Preston↗