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NASA NTRS2017

Risk-Significant Adverse Condition Awareness Strengthens Assurance of Fault Management Systems

As spaceflight systems increase in complexity, Fault Management (FM) systems are ranked high in risk-based assessment of software criticality, emphasizing the importance of establishing highly competent domain expertise to provide assurance. Adverse conditions (ACs) and specific vulnerabilities encountered by safety- and mission-critical software systems have been identified through efforts to reduce the risk posture of software-intensive NASA missions. Acknowledgement of potential off-nominal conditions and analysis to determine software system resiliency are important aspects of hazard analysis and FM. A key component of assuring FM is an assessment of how well software addresses susceptibility to failure through consideration of ACs. Focus on significant risk predicted through experienced analysis conducted at the NASA Independent Verification & Validation (IV&V) Program enables the scoping of effective assurance strategies with regard to overall asset protection of complex spaceflight as well as ground systems. Research efforts sponsored by NASAs Office of Safety and Mission Assurance (OSMA) defined terminology, categorized data fields, and designed a baseline repository that centralizes and compiles a comprehensive listing of ACs and correlated data relevant across many NASA missions. This prototype tool helps projects improve analysis by tracking ACs and allowing queries based on project, mission type, domain/component, causal fault, and other key characteristics. Vulnerability in off-nominal situations, architectural design weaknesses, and unexpected or undesirable system behaviors in reaction to faults are curtailed with the awareness of ACs and risk-significant scenarios modeled for analysts through this database. Integration within the Enterprise Architecture at NASA IV&V enables interfacing with other tools and datasets, technical support, and accessibility across the Agency. This paper discusses the development of an improved workflow process utilizing this database for adaptive, risk-informed FM assurance that critical software systems will safely and securely protect against faults and respond to ACs in order to achieve successful missions.

IV&V↗

NASA NTRS2017

Risk-Significant Adverse Condition Awareness Strengthens Assurance of Fault Management Systems

As spaceflight systems increase in complexity, Fault Management (FM) systems are ranked high in risk-based assessment of software criticality, emphasizing the importance of establishing highly competent domain expertise to provide assurance. Adverse conditions (ACs) and specific vulnerabilities encountered by safety- and mission-critical software systems have been identified through efforts to reduce the risk posture of software-intensive NASA missions. Acknowledgement of potential off-nominal conditions and analysis to determine software system resiliency are important aspects of hazard analysis and FM. A key component of assuring FM is an assessment of how well software addresses susceptibility to failure through consideration of ACs. Focus on significant risk predicted through experienced analysis conducted at the NASA Independent Verification Validation (IVV) Program enables the scoping of effective assurance strategies with regard to overall asset protection of complex spaceflight as well as ground systems. Research efforts sponsored by NASA's Office of Safety and Mission Assurance defined terminology, categorized data fields, and designed a baseline repository that centralizes and compiles a comprehensive listing of ACs and correlated data relevant across many NASA missions. This prototype tool helps projects improve analysis by tracking ACs and allowing queries based on project, mission type, domaincomponent, causal fault, and other key characteristics. Vulnerability in off-nominal situations, architectural design weaknesses, and unexpected or undesirable system behaviors in reaction to faults are curtailed with the awareness of ACs and risk-significant scenarios modeled for analysts through this database. Integration within the Enterprise Architecture at NASA IVV enables interfacing with other tools and datasets, technical support, and accessibility across the Agency. This paper discusses the development of an improved workflow process utilizing this database for adaptive, risk-informed FM assurance that critical software systems will safely and securely protect against faults and respond to ACs in order to achieve successful missions.

Fault management↗

NASA NTRS1991

Next generation earth-to-orbit space transportation systems: Unmanned vehicles and liquid/hybrid boosters

The United States civil space effort when viewed from a launch vehicle perspective tends to categorize into pre-Shuttle and Shuttle eras. The pre-Shuttle era consisted of expendable launch vehicles where a broad set of capabilities were matured in a range of vehicles, followed by a clear reluctance to build on and utilize those systems. The Shuttle era marked the beginning of the U.S. venture into reusable space launch vehicles and the consolidation of launch systems used to this one vehicle. This led to a tremendous capability, but utilized men on a few missions where it was not essential and compromised launch capability resiliency in the long term. Launch vehicle failures, between the period of Aug. 1985 and May 1986, of the Titan 34D, Shuttle Challenger, and the Delta vehicles resulted in a reassessment of U.S. launch vehicle capability. The reassessment resulted in President Reagan issuing a new National Space Policy in 1988 calling for more coordination between Federal agencies, broadening the launch capabilities and preparing for manned flight beyond the Earth into the solar system. As a result, the Department of Defense (DoD) and NASA are jointly assessing the requirements and needs for this nations's future transportation system. Reliability/safety, balanced fleet, and resiliency are the cornerstone to the future. An insight is provided into the current thinking in establishing future unmanned earth-to-orbit (ETO) space transportation needs and capabilities. A background of previous launch capabilities, future needs, current and proposed near term systems, and system considerations to assure future mission need will be met, are presented. The focus is on propulsion options associated with unmanned cargo vehicles and liquid booster required to assure future mission needs will be met.

Hueter, Uwe↗

NASA NTRS2016

Assessing Woody Vegetation Trends in Sahelian Drylands Using MODIS Based Seasonal Metrics

Woody plants play a major role for the resilience of drylands and in peoples' livelihoods. However, due to their scattered distribution, quantifying and monitoring woody cover over space and time is challenging. We develop a phenology driven model and train/validate MODIS (MCD43A4, 500m) derived metrics with 178 ground observations from Niger, Senegal and Mali to estimate woody cover trends from 2000 to 2014 over the entire Sahel. The annual woody cover estimation at 500 m scale is fairly accurate with an RMSE of 4.3 (woody cover %) and r(exp 2) = 0.74. Over the 15 year period we observed an average increase of 1.7 (+/- 5.0) woody cover (%) with large spatial differences: No clear change can be observed in densely populated areas (0.2 +/- 4.2), whereas a positive change is seen in sparsely populated areas (2.1 +/- 5.2). Woody cover is generally stable in cropland areas (0.9 +/- 4.6), reflecting the protective management of parkland trees by the farmers. Positive changes are observed in savannas (2.5 +/- 5.4) and woodland areas (3.9 +/- 7.3). The major pattern of woody cover change reveals strong increases in the sparsely populated Sahel zones of eastern Senegal, western Mali and central Chad, but a decreasing trend is observed in the densely populated western parts of Senegal, northern Nigeria, Sudan and southwestern Niger. This decrease is often local and limited to woodlands, being an indication of ongoing expansion of cultivated areas and selective logging.We show that an overall positive trend is found in areas of low anthropogenic pressure demonstrating the potential of these ecosystems to provide services such as carbon storage, if not over-utilized. Taken together, our results provide an unprecedented synthesis of woody cover dynamics in theSahel, and point to land use and human population density as important drivers, however only partially and locally offsetting a general post-drought increase.

Brandt, Martin↗

NASA NTRSDate not supplied

Hail Storm Risk Assessment Using Space-Borne Remote Sensing Observations and Reanalyses

Much of the world is impacted by severe thunderstorms, but whether they become disasters depends upon resilience--our capacity to prepare, mitigate, respond, and recover. Hail is the costliest severe weather hazard for the insurance industry, generating ~70% of severe convective storm losses due to damage to assets such as homes, businesses, agriculture, and infrastructure. Most insurance companies do not reserve enough capital to cover catastrophes, so they acquire reinsurance. The reinsurance industry uses catastrophe models (CatModels) to statistically estimate risk to an insurer’s portfolio. Hail CatModels are developed with climatologies that define hailstorm frequency and severity. Hail-prone areas can be defined using hail reports from trained spotters, the media, and the general public. Extremely severe hail (2+ inch diameter) occurs nearly every day across the world. Weather radars can detect hail because hailstones strongly reflect microwave signals that they emit. However, hail climatologies are difficult to derive because hail covers small areas and there are neither hail reporting mechanisms (e.g. website or mobile app) nor radar networks in most places outside the US and Europe. This lack of ground truth on severe hail puts society and economies at risk. Hail is generated within storms by strong updrafts. These updrafts exhibit unique signatures in NASA and other agency satellite observations, offering new opportunities for hailstorm analysis. Geostationary (GEO) visible and infrared imagery has been collected for ~15-25 years across the world (region dependent) and methods have been developed at NASA Langley Research Center (LaRC) to detect hailstorm updrafts using GEO imagery. Climatological GEO updraft data has been used by Willis Towers Watson (WTW), a leader in catastrophe risk assessment for the insurance industry, and Karlsruhe Institute of Technology to develop CatModels over Europe and Australia. Hail can also be inferred with passive microwave imagery collected by low-Earth-orbiting sensors such as the GPM GMI, TRMM TMI, AMSR-E, AMSR-2, SSM/I, and SSMIS over the last 20+ years using methods developed at the Marshall Space Flight Center (MSFC). Hailstorms generate enhanced lightning flash rates that can be tracked using new GOES-R series GEO Lightning Mapping (GLM) imagery. Atmospheric reanalyses can be used to define favorable hailstorm environments for combination with the satellite-based storm detections. This presentation will describe a framework for developing continental to global hail climatologies and CatModels based on NASA satellite data and capabilities. This is a collaboration between LaRC and MSFC, WTW, and partners in Brazil, Argentina, and South Africa. This project seeks to mitigate hail disasters by aiding development of new satellite-based severe storm nowcasting tools by regional partners and developing climatologies to improve societal understanding of hail frequency. GEOO visible and infrared metrics of storm intensity, environmental conditions based on reanalyses, spotter hail reports and radar MESH observations are intercompared to quantify the detectability of hailstorms, and our ability to discriminate hailstorms from other severe storms. We are also maturing methods using land surface imaging satellite data (e.g. MODIS, Landsat, Sentinel 1 and 2) to identify hail damage to agriculture. Work with WTW will improve socioeconomic resilience through development of new CatModels. Southern Brazil, Uruguay, Paraguay, and Argentina feature some of the most intense thunderstorms on Earth. South America and South Africa are developing insurance markets of interest to WTW clients, and is similar to other regions routinely impacted by hail that do not have comprehensive hail reporting or radars to assess hailstorm frequency. Project datasets will be made available via online GIS-enabled tools developed at the LaRC Atmospheric Science Data Center (ASDC) which will visualize data and provide it in multiple formats for use in a wide range of open source and commercial tools.

Kristopher Michael Bedka↗

NASA NTRS2024

Fault Diagnosis of Power Components with Reliability Assessment in Extraterrestrial Microgrids

This research investigates the possible failures caused by aging and other environmental and external factors that could significantly impact the performance of extraterrestrial power systems. Additionally, it presents a reliability assessment model for the space microgrid based on fault tree analysis (FTA). The reliability assessment model developed in this paper represents a tool that can be used by engineers to harden the system design for operational and economic benefits. To improve the reliability of the system, this work provides a broad review of the different fault detection and diagnosis (FDD) algorithms used for power microgrids and space applications. Using data sets from the Habitat Simulator developed through the NASA-funded Resilient Extraterrestrial Habitat Institute, this paper compares the applicability and accuracy of the different FDD methods. The primary FDD approach proposed and assessed in this work is based on the Markov reliability model. It predicts and detects future faults in the space microgrids by using past data samples and categorizing them into different classes. Data-driven-based models such as artificial neural networks are also investigated, tested, and evaluated using simulation data sets. According to the simulation results and the broad FDD algorithm comparison, this study provides the crew or maintenance engineers with a clear methodology to detect and localize power system failures.

Leila Chebbo↗

NASA NTRSDate not supplied

Creating Formal Characterizations of Routine Contingency Management in Commercial Aviation

The identification, modelling, and analysis of root causes of accidents and incidents dominate conventional safety management approaches. However, the effect of humans’ safety-producing behavior on the overall resilience of the system is often neglected. Additionally, emerging aviation markets are giving rise to concepts of operation, such as urban air mobility and optionally piloted air cargo operations, that are leading to a shift in locus of control between humans and automation. Without an understanding of the human contribution to safety, it is difficult to assess the effects of these novel role allocations on overall system safety. In this work, safety-producing behaviors are identified and abstracted into resilient performance strategies. Production rules that encapsulate these strategies are then generated and classified in the Soar cognitive architecture. The strategies are then applied to a remotely-operated air cargo example to demonstrate how safe learning is facilitated. The learned rules and strategies are then formally verified.

Safety Critical Systems↗

NASA NTRS2021

Fairfax County Urban Development: Identifying Urban Heat Mitigation Strategies for Climate Adaptation Planning in Fairfax County, Virginia

Extreme high temperatures lead to increased instances of cardiovascular disease, pulmonary disease, and even death, as well as increased energy consumption and infrastructure costs. People in urbanized areas experience higher temperatures than rural areas due to diminished vegetation and increased impervious surfaces which absorb and radiate heat. Fairfax County, Virginia has embarked on Resilient Fairfax, a program aimed at addressing climate adaptation and resilience. The DEVELOP team partnered with the Fairfax County Office of Environmental and Energy Coordination (OEEC) to assess the extent of the urban heat island effect on the county and its most vulnerable populations. The team used data from Landsat 8 Operational Land Imager (OLI) and Thermal Infrared Sensor (TIRS), as well as the ECOsystem Spaceborne Thermal Radiometer Experiment on Space Station (ECOSTRESS) for the years 2013 to 2021 and found that the hottest spots were in densely urbanized areas, with temperatures as much as 47°F above that of undeveloped reference areas. The team used the Integrated Valuation of Ecosystem Services and Tradeoffs (InVEST) urban cooling model and determined that areas with higher tree canopy cover had greater heat mitigation capacity. Estimates from the InVEST model showed that a 4.5% increase in canopy cover across the county could result in a temperature reduction of up to 2.4°F in some areas. The results will allow partners to assess heat distribution across Fairfax County and implement effective mitigation strategies, including locating prime locations for cooling centers and increasing canopy cover.

W. Pierce Holloway↗

NASA NTRS2009

A Landsat-Based Assessment of Mobile Bay Land Use and Land Cover Change from 1974 to 2008

The Mobile Bay region has experienced noteworthy land use and land cover (LULC) change in the latter half of the 20th century. Accompanying this change has been urban expansion and a reduction of rural land uses. Much of this LULC change has reportedly occurred since the landfall of Hurricane Frederic in 1979. The Mobile Bay region provides great economic and ecologic benefits to the Nation, including important coastal habitat for a broad diversity of fisheries and wildlife. Regional urbanization threatens the estuary s water quality and aquatic-habitat dependent biota, including commercial fisheries and avian wildlife. Coastal conservation and urban land use planners require additional information on historical LULC change to support coastal habitat restoration and resiliency management efforts. This presentation discusses results of a Gulf of Mexico Application Pilot project that was conducted in 2008 to quantify and assess LULC change from 1974 to 2008. This project was led by NASA Stennis Space Center and involved multiple Gulf of Mexico Alliance (GOMA) partners, including the Mobile Bay National Estuary Program (NEP), the U.S. Army Corps of Engineers, the National Oceanic and Atmospheric Administration s (NOAA s) National Coastal Data Development Center (NCDDC), and the NOAA Coastal Services Center. Nine Landsat images were employed to compute LULC products because of their availability and suitability for the application. The project also used Landsat-based national LULC products, including coastal LULC products from NOAA s Coastal Change & Analysis Program (C-CAP), available at 5-year intervals since 1995. Our study was initiated in part because C-CAP LULC products were not available to assess the region s urbanization prior to 1995 and subsequent to post Hurricane Katrina in 2006. This project assessed LULC change across the 34-year time frame and at decadal and middecadal scales. The study area included the majority of Mobile and Baldwin counties that encompass Mobile Bay. In doing so, each date of Landsat data was classified using an end-user defined modified Anderson level 1 classification scheme. LULC classifications were refined using a decision rule approach in conjunction with available C-CAP products. Individual dates of LULC classifications were validated by image interpretation of stratified random locations on raw Landsat color composite imagery in combination with higher resolution remote sensing and in-situ reference data. The results indicate that during the 34-year study period, urban areas increased from 96,688 to 150,227 acres, representing a 55.37% increase, or 1.63% per annum. Most of the identified urban expansion results from conversion of rural forest and agriculture to urban cover types. Final LULC mapping and metadata products were produced for the entire study area as well as watersheds of concern within the study area. Final project products, including LULC trend information, were incorporated into the Mobile Bay NEP State of the Bay report. Products and metadata were transferred to NOAA NCDDC to allow free online accessibility and use by GOMA partners and by the public.

Spruce, Joseph↗

NASA NTRS2020

Threats to Resiliency of Redundant Systems Due to Destructive SEE

Destructive SEE pose serious challenges for the reliable use of COTS devices in space systems. We used system-level modeling to determine SEL rates that would likely compromise system reliability, resilience and capabilities. We then assembled a representative dataset of COTS CMOS parts and used nonparametric statistical techniques to assess the threat posed to redundant systems by destructive SEE.

single-event effects↗

NASA NTRSDate not supplied

Technology Assessment and Modelling of Three Terminal Tandem Solar Cells for In-Space Utilization

NASA seeks a sustained human presence on the lunar surface. High-efficiency and resilient energy systems are critical to supporting habitats, scientific outposts, and lunar surface operations. Photovoltaics have long been the backbone of space power systems, transitioning from silicon solar cells to multi-junction III-V solar cells, which now dominate state-of-the-art (SOA) technology. Multi-junction cells achieve high efficiency by layering semiconductors, each absorbing a specific portion of the solar spectrum. However, efficiency gains are increasingly constrained by device physics and manufacturing complexity. Additionally, higher-order junctions pose challenges for accurate modelling and performance characterization due to difficulties in replicating the AM0 spectrum.

solar cells↗

NASA NTRS2017

Preliminary Investigation of Impact of Technological Impairment on Trajectory-Based Operations

The Next Generation Air Transportation System (NextGen) incorporates collaborative air traffic management and Trajectory-Based Operations (TBO) in order to significantly increase the capacity, efficiency, and predictability of operations in the National Airspace System (NAS), without decreasing safety. This is enabled by airspace users and service providers sharing knowledge about operations that allows prediction of the complete 4D flight trajectory with as little uncertainty as possible. Additionally, new software and hardware technology is critical to reaching NextGen goals, especially with regard to TBO. What if the technologies that are critical for TBO were to be impaired or fail completely? Should there be a malfunction of a piece of the technology, it must be ensured that the whole system does not break down completely or suffer severe impairment. Instead, operations need to be maintained proportionally to the problem and safety needs to be ensured (graceful degradation). This paper proposes a systematic framework to investigate the vulnerability of TBO to technology disruption, and determine the impact of technological impairment on TBO. Two representative technologies are chosen for detailed investigation and the impact of their impairment on the degradation of TBO is illustrated using a weather-related scenario. XXXX There are several possible directions of future work. We believe it is desirable to develop methods to quantitatively assess the impact of technological disruption on TBO and to have the simulation tools to validate the impact. The availability of prognostics and health management methods could be leveraged to predict technological failure/disruption, thus predicting how TBO will be a ected, and possibly pro-actively mitigating the impact. It is important to develop large-scale scenarios where the e ect of technological impairment is prominent, and identify methods to quantitatively assess the extent of TBO degradation. An important goal of such an investigation is the development of failure-resistant resilient trajectory-based oper- ations. Resilience14, 15 is the property of a system to \bounce back" and resume at least a signi cant portion of its functionalities after degradation due to technological impairment(s). A systems resilience includes properties such as \bu ering capacity" (quantifying disruptions the system can absorb or adapt to without a fundamental breakdown in performance or in the systems structure), \ exibility" (ability to restructure itself in response to external changes or pressures), "margin" (how closely the system is currently operating rela- tive to one or another kind of performance boundary), \tolerance" (whether the system gracefully degrades as stress/pressure increase, or collapses quickly when pressure exceeds adaptive capacity), etc. Future work needs to focus on quantifying and improving the resilience of TBO, and identifying resilient design solutions for aviation.

TBO↗

NASA NTRSDate not supplied

Enterococci in Space: Adaptation, Antibiotic Resistance, and Clinical Implications

Enterococci are gram-positive bacteria that originated when our ancient animal ancestors emerged from the oceans to live on land, and brought their gut flora with them. Enterococcus faecalis (EF) and Enterococcus faecium, are common human commensals and can harbor multidrug resistance. Both have been previously isolated from the International Space Station (ISS). Likely as a consequence of their evolutionary origins, enterococci show remarkable stress resistance within, but also outside, their human hosts. Their antibiotic resistance, coupled with tolerance to desiccation, starvation, and disinfection, make some EF strains potent pathogens in the built environment (e.g., hospitals), and a potential risk to crew health during space missions. Here we describe our planned flight studies, currently in development. Genomic Enumeration of Antibiotic Resistance in Space (GEARS) will characterize the frequency and genomic identity of antibiotic resistant organisms, including enterococci, on the ISS, and expand our future in-space sequencing-based diagnostic capabilities. Enterococcus Growth Advantage on ISS via Tn-seq (EnteroGAIT) will assess the evolutionary selective pressure of the space environment (microgravity, space radiation) using EF as a model system during a long-duration culture and persistence experiment. Adaptation & Evolution of Resilient Enterococcus in Space (AERES) will study existing and newly identified isolates to characterize the “natural” evolutionary history of EF on Earth and in space to reveal mechanisms of microbial adaption including, possibly, natural selection. Our work to date has identified that EF ISS isolates share virulence factors found in clinical strains and commensals, and revealed limitations of current pathogenicity assessment tools. While ISS isolates were, in some cases, multi-drug resistant, current data suggests these are largely commensal strains. Our work will further refine potential crew health risks and improve understanding of EF adaptation to the built environment, of great relevance on Earth where EF is the second leading cause of hospital acquired infection.

Jordan McKaig↗

NASA NTRS2010

Near Real-Time Optimal Prediction of Adverse Events in Aviation Data

The prediction of anomalies or adverse events is a challenging task, and there are a variety of methods which can be used to address the problem. In this paper, we demonstrate how to recast the anomaly prediction problem into a form whose solution is accessible as a level-crossing prediction problem. The level-crossing prediction problem has an elegant, optimal, yet untested solution under certain technical constraints, and only when the appropriate modeling assumptions are made. As such, we will thoroughly investigate the resilience of these modeling assumptions, and show how they affect final performance. Finally, the predictive capability of this method will be assessed by quantitative means, using both validation and test data containing anomalies or adverse events from real aviation data sets that have previously been identified as operationally significant by domain experts. It will be shown that the formulation proposed yields a lower false alarm rate on average than competing methods based on similarly advanced concepts, and a higher correct detection rate than a standard method based upon exceedances that is commonly used for prediction.

Martin, Rodney Alexander↗

NASA NTRSDate not supplied

Examining the Relationship between Self-Reported Workload and Resilient Performance in Airline Flight Crews

Workload has long been associated with human performance in aviation. High workload is typically viewed as a system design problem. The aim of this work was to find observable behaviors that guard cognitive resource margins; thus, improve handling of perturbations that arise. Twelve commercial airline flight crews participated in an experiment at NASA Langley Research Center. We explored workload data (NASA Task Load Index) self-reported after completing flight simulation scenarios to see how different individuals, using the same technologies, cope with challenging situations. A trained observer from the same airline as the study participants assessed performance. We differentiated a lower workload crew from a higher workload crew and analyzed their workload and performance. Results indicate that the workload may be a component of pilots’ resilient performance. Initial strategies that reduced workload were classified and these types of behaviors, if implemented correctly, might assist pilots in maintaining functional workload resource margins.

Michael Stewart↗

NASA NTRSDate not supplied

Examining the Relationship Between Workload and Resilient Performance in Airline Flight Crews

Michael Stewart↗

NASA NTRS2000

The Dynamics of a Semi-Arid Region in Response to Climate and Water - Use Policy

The objectives of this project were to determine the response of semi-arid ecosystems to the combined forcings of climate variability and anthropogenic stress. Arid and semi-arid systems encompass close to 40% of the worlds land surface. The ecology of these regions are principally limited by water, and as the water resources wax and wane, so should the health and vigor of the ecosystems. Water, however, is a necessary and critical resource for humans living in these same regions. Thus for many and and semi-arid regions the natural systems and human systems are in direct competition for a limited resource. Increasing competition through development of and and semi-arid regions, export of water resources, as well as potential persistent changes in weather patterns are likely to lead to fundamental changes in carrying capacity, resilience, and ecology of these regions. A detailed understanding of these systems respond to forcing on a regional and local scale is required in order to better prepare for and manage future changes in the availability of water. In the Owens Valley CA, decadal changes in rainfall and increased use of groundwater resources by Los Angles (which derives 60-70% of its water from this region) have resulted in a large-scale experiment on the impacts of these changes in semi-arid ecosystems. This project works directly with the Inyo County Water Department (local water authority) and the Los Angles Department of Water and Power (regional demand on water resources) to understand changes, their causes, and impacts. Very detailed records have been kept for a number of selected sites in the valley which provide essential ground truth. These results are then scaled up through remote sensed data to regions scale to assess large scale patterns and link them to the fundamental decisions regarding the water resources of this region. A fundamental goal is to understand how resilient the native ecosystems are to large changes in water resources. Are they are on a spring (remove and return resources, do the systems return to the original state) or a vector (when water returns have the systems fundamentally changed).

Mustard, John F.↗

NASA NTRS2012

Gene Expression Measurement Module (GEMM) - A Fully Automated, Miniaturized Instrument for Measuring Gene Expression in Space

The capability to measure gene expression on board spacecraft opens the door to a large number of high-value experiments on the influence of the space environment on biological systems. For example, measurements of gene expression will help us to understand adaptation of terrestrial life to conditions beyond the planet of origin, identify deleterious effects of the space environment on a wide range of organisms from microbes to humans, develop effective countermeasures against these effects, and determine the metabolic bases of microbial pathogenicity and drug resistance. These and other applications hold significant potential for discoveries in space biology, biotechnology, and medicine. Supported by funding from the NASA Astrobiology Science and Technology Instrument Development Program, we are developing a fully automated, miniaturized, integrated fluidic system for small spacecraft capable of in-situ measurement of expression of several hundreds of microbial genes from multiple samples. The instrument will be capable of (1) lysing cell walls of bacteria sampled from cultures grown in space, (2) extracting and purifying RNA released from cells, (3) hybridizing the RNA on a microarray and (4) providing readout of the microarray signal, all in a single microfluidics cartridge. The device is suitable for deployment on nanosatellite platforms developed by NASA Ames' Small Spacecraft Division. To meet space and other technical constraints imposed by these platforms, a number of technical innovations are being implemented. The integration and end-to-end technological and biological validation of the instrument are carried out using as a model the photosynthetic bacterium Synechococcus elongatus, known for its remarkable metabolic diversity and resilience to adverse conditions. Each step in the measurement process-lysis, nucleic acid extraction, purification, and hybridization to an array-is assessed through comparison of the results obtained using the instrument with those from standard laboratory protocols. Once developed, the system can be used with minor modifications for multiple experiments on different platforms in space, including extension to higher organisms and microbial monitoring. A proposed version of GEMM that is capable of handling both microbial and tissue samples on the International Space Station will be briefly summarized.

Pohorille, Andrew↗