Search NASA⌕ Search

SEARCH · Search NASA

Results for “SPHEROID”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 73 records · Page 4
All sourcesNASA NTRSDOE OSTI
DOE OSTI2021

In vitro assessment of a synergistic combination of gemcitabine and zebularine in pancreatic cancer cells

Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest cancers with an extremely poor prognosis. Gemcitabine (Gem) is still the mainstay drug for the treatment of PDAC. However, rapid inactivation by cytidine deaminase (CDA) present in pancreatic cancer cells severely limits anticancer efficacy of Gem. In this study, we investigated the effect of a CDA inhibitor - Zebularine (Zeb) on anticancer activity of Gem in pancreatic cancer cell lines MiaPaCa-2, BxPC-3, and Panc-1. Zeb treatment synergistically increased Gem-induced cytotoxicity in all three pancreatic cancer cell lines. The strongest synergistic activity was found at 1:10 M ratio of Gem/Zeb (combination index 0.04–0.4). Additionally, Gem + Zeb treated cells showed marked decreased in the expressions of anti-apoptotic protein including Bcl-2 and survivin while significantly increased the cleaved caspase-3, and loss of mitochondrial membrane potential was observed. Multicellular 3D spheroids of MiaPaCa-2 cells treated with combination showed significant reduction (25–60%) in spheroid size, weight compared to single drug and control group. Live/dead cell imaging showed that Gem + Zeb treated spheroids exhibited a highly distorted surface with significantly higher number of dead cells (red). The results of the present study confirm that this synergistic combination is worthy of future investigations as a potential approach for the treatment of PDAC.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2021

Revealing the Surface Structure of CdSe Nanocrystals by Dynamic Nuclear Polarization-Enhanced 77 Se and 113 Cd Solid-State NMR Spectroscopy

Dynamic nuclear polarization (DNP) solid-state NMR (SSNMR) spectroscopy was used to obtain detailed surface structures of zinc blende CdSe nanocrystals (NCs) with plate or spheroidal morphologies which are capped by carboxylic acid ligands. 1D 113 Cd and 77 Se cross-polarization magic angle spinning (CPMAS) NMR spectra revealed distinct signals from Cd and Se atoms on the surface of the NCs, and those residing in bulk-like environments, below the surface. 113 Cd cross-polarization magic-angle-turning (CP-MAT) experiments identified CdSe 3 O, CdSe 2 O 2 , and CdSeO 3 Cd coordination environments on the surface of the NCs, where the oxygen atoms are presumably from coordinated carboxylate ligands. The sensitivity gain from DNP enabled natural isotopic abundance 2D homonuclear 113 Cd– 113 Cd and 77 Se– 77 Se and heteronuclear 113 Cd– 77 Se scalar correlation solid-state NMR experiments which revealed the connectivity of the Cd and Se atoms. Importantly, 77 Se{ 113 Cd} scalar heteronuclear multiple quantum coherence ( J -HMQC) experiments were used to selectively measure one-bond 77 Se– 113 Cd scalar coupling constants ( 1 J ( 77 Se, 113 Cd)). With knowledge of 1 J ( 77 Se, 113 Cd), heteronuclear 77 Se{ 113 Cd} spin echo ( J -resolved) NMR experiments were used to determine the number of Cd atoms bonded to Se atoms and vice versa. Furthermore, the J -resolved experiments directly confirmed that major Cd and Se surface species have CdSe 2 O 2 and SeCd 4 stoichiometries, respectively. Considering the crystal structure of zinc blende CdSe and the similarity of the solid-state NMR data for the platelets and spheroids, we conclude that the surface of the spheroidal CdSe NCs is primarily composed of {100} facets. The methods outlined here will generally be applicable to obtain detailed surface structures of various main group semiconductor nanoparticles.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
DOE OSTI2020

Cuboidal Liquid Crystal Phases Under Multiaxial Geometrical Frustration.

Cuboidal liquid crystal phases - the so-called blue phases - consist of a network of topological defects arranged into a cubic symmetry. They exhibit striking optical properties, including Bragg reflection in the visible range and fast response times. Confining surfaces can interfere with the packing of such a network, leading to structures that have not been explored before. In this work, a Landau-de Gennes free energy formalism for the tensor alignment field Q is used to investigate the behavior of chiral liquid crystals under non-isotropic confinement. The underlying free energy functional is solved by relying on a Monte Carlo method that facilitates efficient exploration of configuration space. The results of simulations are expressed in terms of phase diagrams as a function of chirality and temperature for three families of spheroids: oblate, spherical, and prolate. Upon deformation, blue phases adapt and transform to accommodate the geometrical constraints, thereby resulting in a wider range of thermal stability. For oblate spheroids, confinement interferes with the development of a full blue phase structure, resulting on a combination of half skyrmions. For prolate spheroids, the blue phases are hybridized and exhibit features of blue phases I and II. More generally, it is shown that mechanical deformation provides an effective means to control, manipulate and stabilize blue phases and cholesterics confined in tactoids.

Palacio-Betancur, Viviana↗
DOE OSTI2022

Extremely massive disc galaxies in the nearby Universe form through gas-rich minor mergers

ABSTRACT In our hierarchical structure-formation paradigm, the observed morphological evolution of massive galaxies – from rotationally supported discs to dispersion-dominated spheroids – is largely explained via galaxy merging. However, since mergers are likely to destroy discs, and the most massive galaxies have the richest merger histories, it is surprising that any discs exist at all at the highest stellar masses. Recent theoretical work by our group has used a cosmological, hydrodynamical simulation to suggest that extremely massive (M* > 1011.4 M⊙) discs form primarily via minor mergers between spheroids and gas-rich satellites, which create new rotational stellar components and leave discs as remnants. Here, we use UV-optical and H i data of massive galaxies, from the Sloan Digital Sky Survey, Galaxy Evolution Explorer, Dark Energy Camera Legacy Survey (DECaLS), and Arecibo Legacy Fast ALFA surveys, to test these theoretical predictions. Observed massive discs account for ∼13 per cent of massive galaxies, in good agreement with theory (∼11 per cent). ∼64 per cent of the observed massive discs exhibit tidal features, which are likely to indicate recent minor mergers, in the deep DECaLS images (compared to ∼60 per cent in their simulated counterparts). The incidence of these features is at least four times higher than in low-mass discs, suggesting that, as predicted, minor mergers play a significant (and outsized) role in the formation of these systems. The empirical star formation rates agree well with theoretical predictions and, for a small galaxy sample with H i detections, the H i masses and fractions are consistent with the range predicted by the simulation. The good agreement between theory and observations indicates that extremely massive discs are indeed remnants of recent minor mergers between spheroids and gas-rich satellites.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2025

Investigation of In Situ and Ex Situ Passivation of Pyrophoric Uranium–Niobium Alloy Powder

This work evaluates the effectiveness of in situ and ex situ passivation methods for mitigating the pyrophoricity of uranium–6 wt.% niobium spherical powders produced via the hydride–dehydride process coupled with plasma spheroidization. Oxide layer thickness was characterized using STEM/EDX, and pyrophoricity was assessed by a UN-recommended test method, which involves directly dropping the powders in the air. In situ passivation, performed by introducing flowing oxygen during spheroidization, produced oxide layers ranging from tens to hundreds of nanometers but resulted in inconsistent pyrophoricity mitigation at lower oxygen flow rates. Ex situ passivation, achieved by slow oxygen exposure over several months, formed uniform oxide layers of approximately 20 nm and consistently mitigated pyrophoricity. Despite requiring higher bulk oxygen content, in situ passivation enables faster processing and control of oxygen, while ex situ passivation achieves superior oxide uniformity with lower oxygen incorporation. These findings highlight the trade-offs between passivation methods and provide a foundation for improving the safety and scalability of reactive metal powder production.

U-6Nb↗
DOE OSTI2020

A systematic study of hidden sector dark matter: application to the gamma-ray and antiproton excesses

In hidden sector models, dark matter does not directly couple to the particle content of the Standard Model, strongly suppressing rates at direct detection experiments, while still allowing for large signals from annihilation. In this paper, we conduct an extensive study of hidden sector dark matter, covering a wide range of dark matter spins, mediator spins, interaction diagrams, and annihilation final states, in each case determining whether the annihilations are s-wave (thus enabling efficient annihilation in the universe today). We then go on to consider a variety of portal interactions that allow the hidden sector annihilation products to decay into the Standard Model. We broadly classify constraints from relic density requirements and dwarf spheroidal galaxy observations. In the scenario that the hidden sector was in equilibrium with the Standard Model in the early universe, we place a lower bound on the portal coupling, as well as on the dark matter’s elastic scattering cross section with nuclei. We apply our hidden sector results to the observed Galactic Center gamma-ray excess and the cosmic-ray antiproton excess. We find that both of these excesses can be simultaneously explained by a variety of hidden sector models, without any tension with constraints from observations of dwarf spheroidal galaxies.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗
DOE OSTI2023

Microstructure and strengthening of Al-6Ce-3Ni-0.7Fe (wt%) alloy manufactured by laser powder-bed fusion

In this study, an Al-6Ce-3Ni-0.7Fe (wt%) alloy was fabricated via laser powder-bed fusion and its microstructure, thermal stability, tensile properties, and creep properties are investigated for two rapid-solidification rates with different eutectic spacings. For both faster- and slower-cooled states, the as-fabricated alloy mostly shows elongated grains with very fine eutectic networks (~40 nm lamellar width) comprising a high-volume fraction (~18 %) of intermetallic phases. Faster-cooled samples, however, display a less continuous eutectic network with finer spacing (~100 nm), leading to higher Orowan strengthening and therefore superior microhardness and yield stress up to ~350 °C. Upon aging (300 - 450 °C), micron-size Al 9 (Ni,Fe) 2 needle-like precipitates form within grains, and the Al 11 Ce 3 eutectic network spheroidizes, while retaining a submicron width and spacing, resulting in 33–37 % drop in microhardness after 144 h aging at 400 °C. Limited tensile ductility (~6 %) and creep ductility (~1-2 %) are measured for both faster- and slower-cooled alloys due to an inhomogeneous microstructure, where cavitation preferentially initiates at melt-pool boundaries, precipitate-free zones, and/or denuded zones, eventually leading to local fracture. Denuded zones, induced by stress, form due to diffusional flow with stress-dependent orientations, and are identified as microstructurally weak regions leading to strain localization. The present alloy does not show a significant difference in 300 °C creep resistance between: (i) tensile and compressive loading, (ii) faster- and slower-cooled samples, and (iii) continuous and spheroidized eutectics. Creep resistance at 300 °C is comparable to that of a Ce-richer Al-10.5Ce-3.1Ni-1.2Mn (wt%) alloy, despite the formation of denuded zones and needle-like Al 9 (Ni,Fe) 2 precipitates.

36 MATERIALS SCIENCE↗
DOE OSTI2021

Unraveling compacted graphite evolution during solidification of cast iron using in-situ synchrotron X-ray tomography

In spite of many years of research, the physical phenomena leading to the evolution of compacted graphite (CG) during solidification is still not fully understood. In particular, it is unknown how highly branched CG aggregates form and evolve in the semi-solid, and how local microstructural variations at micrometer length scale affect this growth process. We present here the first time-resolved synchrotron tomography combined with a bespoke high-temperature environmental cell that allows direct observation of the evolution of CG and relates this dynamic process to the local surrounding microstructures in a cast iron sample during repeated melting and solidification. Distinct processes are identified for the formation of CG involving the nucleation, growth, development of branches and interconnection of graphite particles, ultimately evolving into highly branched graphite aggregates with large sizes and low sphericities. CG is found to nucleate with a spheroidal or a plate-like shape, developing branches induced by high carbon concentration, e.g. thin melt channels. Additionally, CG grows much faster than spheroidal graphite during subsequent cooling in solid state. The direct visualization of the dynamic solidification process provides unprecedented new insights into formation mechanisms of CG and correlating factors such as local microstructural variations, and guides the development of CG iron solidification models.

36 MATERIALS SCIENCE↗
DOE OSTI2022

Metallic Technetium Sequestration in Nickel Core/Shell Microstructure during Fe(OH)2 Transformation with Ni doping

This study investigates the impacts of Ni doping on technetium-99 (Tc) sequestration in aqueous solutions through transformation of Fe(OH)2(s) to iron spinel (magnetite) under alkaline conditions. Extensive solid characterization was performed for the mineral phases produced, as well as the Tc/Ni speciation and distribution within these phases. X-ray diffraction results show that iron spinel was the dominant mineral product without detectable Ni incorporation. The doped Ni ions mainly precipitated as fine Fe/Ni oxide/hydroxide particles, including strongly reduced nanometer?sized spheroidal Ni-rich and metallic Ni phases. High-resolution analytical scanning transmission electron microscopy using energy dispersive X-ray spectroscopy and electron energy loss spectroscopy on the produced solid samples (focused ion beam-prepared specimens) revealed three Tc distribution domains dominated by nanocrystals and, especially, a Tc-rich metallic phase. Instances of metallic Tc were specifically found in spheroidal, Ni-rich and metallic nanoparticles exhibiting a core/shell microstructure that suggests strong reduction and sequential precipitation of Ni-Tc-Ni. Mass balance analysis showed nearly 100% Tc removal from the 4.8 × 10-4 M Tc solutions. The finding of the metallic Tc encapsulation indicates that Tc sequestration through Ni-doped Fe(OH)2(s)?to?iron spinel transformation process likely provides an alternative treatment pathway for Tc removal and could be combined into further waste treatment approaches.

Wang, Guohui↗
DOE OSTI2024

Superhydrophobic Array Devices for the Enhanced Formation of 3D Cancer Models

During the metastatic cascade, cancer cells travel through the bloodstream as circulating tumor cells (CTCs) to a secondary site. Clustered CTCs have greater shear stress and treatment resistance, yet their biology remains poorly understood. We therefore engineered a tunable superhydrophobic array device (SHArD). The SHArD-C was applied to culture a clinically relevant model of CTC clusters. Using our device, we cultured a model of cancer cell aggregates of various sizes with immortalized cancer cell lines. These exhibited higher E-cadherin expression and are significantly more capable of surviving high fluid shear stress-related forces compared to single cells and model clusters grown using the control method, helping to explain why clustering may provide a metastatic advantage. Additionally, the SHArD-S, when compared with the AggreWell 800 method, provides a more consistent spheroid-forming device culturing reproducible sizes of spheroids for multiple cancer cell lines. Overall, we designed, fabricated, and validated an easily tunable engineered device which grows physiologically relevant three-dimensional (3D) cancer models containing tens to thousands of cells.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
DOE OSTI2022

Dark and luminous mass components of Omega Centauri from stellar kinematics

Here we combine proper motion data from Gaia EDR 3 and HST with line-of-sight velocity data to study the stellar kinematics of the ω Cen globular cluster. Using a steady-state, axisymmetric dynamical model, we measure the distribution of both the dark and luminous mass components. Assuming both Gaussian and Navarro–Frenk–White mass profiles, depending on the data set, we measure an integrated mass of ≲10 6 M ⊙ within the ω Cen half-light radius for a dark component that is distinct from the luminous stellar component. For the HST and radial velocity data, models with a non-luminous mass component are strongly statistically preferred relative to a stellar mass-only model with a constant mass-to-light ratio. While a compact core of stellar remnants may account for a dynamical mass up to ~5 x 10 5 M ⊙ , they likely cannot explain the higher end of the range. This leaves open the possibility that this non-luminous dynamical mass component comprises non-baryonic dark matter. In comparison to the dark matter distributions around dwarf spheroidal galaxies, the ω Cen dark mass component is much more centrally concentrated. Interpreting the non-luminous mass distribution as particle dark matter, we use these results to obtain the J-factor, which sets the sensitivity to the annihilation cross-section. For the data sets considered, the range of median J-factors is ~10 22 -10 24 GeV 2 cm -5 , which is larger than that obtained for any dwarf spheroidal galaxy.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2024

New dark matter analysis of milky way dwarf satellite galaxies with madhatv2

We obtain bounds on dark matter annihilation using 14 years of publicly available Fermi-LAT data from a set of 54 dwarf spheroidal galaxies, using spectral information from 16 energy bins. We perform this analysis using our updated and publicly available code , which can be used to test a variety of models for dark matter particle physics and astrophysics in an accessible manner. In particular, we note that including Carina III in the analysis strengthens constraints on s -wave annihilation into two-body Standard Model final states by a factor of ∼ 3 but broadens the error on the constraint due to the large uncertainty of its J -factor. Our findings illustrate the importance of verifying if Carina III is in fact a dwarf spheroidal galaxy and measuring more precisely its J -factor. More generally, they highlight the significance of forthcoming discoveries of nearby ultrafaint dwarfs for dark matter indirect detection. Published by the American Physical Society 2024

Astronomy & Astrophysics↗
DOE OSTI2021

An Investigation of the Effects of Volume Fraction on Drag Coefficient of Non-Spherical Particles Using PR-DNS

Prediction of the drag coefficient is required in gas-particle multiphase flow modeling and simulation. Experimental data and correlations on the fixed-bed system of spherical particles with high volume fractions for various possible arrangements are available in the literature. However, the effect of volume fraction on the drag coefficient of non-spherical particles is not well studied. In solving the momentum equation, the volume fraction plays a vital role in determining the flow resistances. In this paper, we study the impact of volume fraction in the range of 0.069 to 0.65 on the drag coefficient using the computational fluid dynamics (CFD) simulation of air for Reynold number in the range of 10 to 10000 using particle resolved direct numerical solution (PR-DNS). Regular non-spherical particles such as a cube, tetrahedron, and spheroids are used in this study since their single particle’s drag coefficient data are available in the literature for comparison. For this work, the simulations are carried out in the Ansys Fluent using polyhedral mesh, which consumes significantly less computational time and power. The study showed the sphericity and volume fraction have significant impact on the bed pressure drop and average drag coefficient of the particles in the bed especially in high Reynolds number regime. The bed of the spheroid experiences the lowest drag being the most streamlined particle, and the particles with the edges result in a large drag coefficient due to flow separation at the discontinuity. The vector plots verify this behavior where large wake regions are observed behind the tetrahedron particle.

Mahyawansi, Pratik↗
DOE OSTI2022

The Isothermal Oxidation of High-Purity Aluminum at High Temperature

The isothermal oxidation in air of high purity aluminum sheet was studied as a function of temperature using Thermogravimetric Analysis simultaneously with Differential Scanning Calorimetry (TGA/DSC). The rates and extents of oxidation were found to be non-linear functions of the temperature, in agreement with the literature. Between 650 °C and 750 °C very little oxidation took place; at 850 °C oxidation occurred after an induction period, while at 950 °C oxidation occurred without an induction period. At oxidation temperatures between 1050 °C and 1150 °C rapid passivation of the surface of the aluminum occurred, while at 1250 °C and above, an initial rapid mass increase was observed, followed by a more gradual increase in mass. The initial rapid increase in mass was accompanied by a significant exotherm, which was quantified by DSC. At temperatures of 1050 °C and above the specimen coalesced into a spheroidal particle, whereas at lower temperatures the original morphology was retained due to the cohesive strength of the native oxide layer. Cross-sections of oxidized specimens were characterized by scanning electron microscopy (SEM); the observed alumina skin thicknesses correlated qualitatively with the observed mass increases. Interrogation of the surface of an oxidized spheroidal particle by SEM showed a fractured alumina shell around a partially hollow core of aluminum which appeared to have grain boundaries.

36 MATERIALS SCIENCE↗
DOE OSTI2022

Beyond Galaxy Bimodality: The Complex Interplay between Kinematic Morphology and Star Formation in the Local Universe

It is generally assumed that galaxies are a bimodal population in both star formation and structure; star-forming galaxies are disks, while passive galaxies host large bulges or are entirely spheroidal. Here we test this scenario by presenting a full census of the kinematic morphologies of a volume-limited sample of galaxies in the local universe extracted from the MaNGA galaxy survey. We measure the integrated stellar line-of-sight velocity to velocity dispersion ratio (V/σ) for 4574 galaxies in the stellar mass range 9.75 < logM $\star$ [M ⊙ ] < 11.75. We show that at fixed stellar mass, the distribution of V/σ is not bimodal, and that a simple separation between fast and slow rotators is oversimplistic. Fast rotators are a mixture of at least two populations, referred to here as dynamically cold disks and intermediate systems, with disks dominating in both total stellar mass and number. When considering star-forming and passive galaxies separately, the star-forming population is almost entirely made up of disks, while the passive population is mixed, implying an array of quenching mechanisms. Passive disks represent ~30% (both in number and mass) of passive galaxies, nearly a factor of two higher than that of slow rotators, reiterating that these are an important population for understanding galaxy quenching. These results paint a picture of a local universe dominated by disky galaxies, most of which become somewhat less rotation-supported upon or after quenching. While spheroids are present to a degree, they are certainly not the evolutionary end point for the majority of galaxies.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2026

Impact of Cosmic Filaments on Galaxy Morphological Evolution and Predictions of Early Cosmic Web Structure for Roman

We leverage the IllustrisTNG cosmological simulations to test how the large-scale cosmic web shapes galaxy morphology and to forecast the early cosmic web structure that the Nancy Grace Roman Space Telescope will reveal. In the hydrodynamic TNG50 and N-body TNG50-Dark runs, we reconstruct the cosmic web at redshifts z = 0, 0.5, 1, 2, 3, and 4 with the Monte Carlo Physarum Machine density estimator and the DisPerSE structure identification framework. We confirm that dark matter halos start out predominantly prolate (elongated), and their shapes are aligned with their nearest filaments; prolate galaxies retain strong shape alignment with their outer halos to later times. At z ≥ 1, the fraction of prolate (spheroidal) halos increases (decreases) toward lower stellar mass, higher redshift, and lower filament density. At z < 1, more spheroidal (oblate) stellar structures preferentially reside in higher-density (lower-density) filaments. We also find that higher-density filaments favor extended rotationally supported disks, whereas lower-density filaments more often host smaller dispersion-supported systems. Then, generating mock galaxy samples from TNG100 and TNG50, we predict the early cosmic web accessible to Roman. We find that the spectroscopic emission-line depth planned for the High-Latitude Wide-Area Survey (HLWAS) yields a highly incomplete galaxy sample that does not accurately trace the z = 1 cosmic web. A survey ≥2.5× deeper over a few square degrees would enable a proper reconstruction and reveal qualitatively correct filament–galaxy morphology relationships. Nevertheless, the planned HLWAS Deep field should still identify most galaxy overdensities; targeted deeper spectroscopy of these regions would efficiently and adequately map the early filamentary structure.

Hasan, Farhanul [Space Telescope Science Institute↗
DOE OSTI2025

Reconfigurable structural color by reversible switching of colloidal discoid liquid crystal alignment

Combining the effects of alternating-current (AC) electric fields and sedimentation reconfigures colloidal discoids between planar and homeotropic orientational alignments, thereby controlling the material’s structural color. Here, we self-assemble micrometer-size polystyrene discoids in an isopropanol-water mixture. After sedimentation, the discoids adopt a homeotropic alignment, with the minor axis perpendicular to the substrate. Adding an AC electric field (1 kHz) of ≥0.50 V switches the discoids to planar alignment—with the minor axis parallel to the substrate—within ∼100 s. Removing the field switches them back to homeotropic alignment within ∼300 s. Kinetic modeling of the field-induced torques yields good agreement with these measurements. The peak wavelength and intensity of the crystal’s diffraction response shift significantly upon reconfiguration; scattering simulation predicts these shifts. The reconfiguration is maintained for at least 10 cycles. This method is a simple, scalable avenue to reconfigure the optical properties of colloidal crystals produced from simple dielectric spheroids.

colloidal crystals↗
DOE OSTI2021

Long noncoding RNA AK023096 interacts with hnRNP-K and contributes to the maintenance of self-renewal in bladder cancer stem-like cells

Highlights: • BCSLCs play a critical role for the high risk of recurrence and we identified that lncRNA-AK023096 was required for controls stemness of BCSLCs. • LncRNA-AK023096 recruits hnRNP-K and epigenetically upregulated SOX2 expression by modulating H3K4 trimethylation of SOX2 promoter. • Overexpression of lncRNA-AK023096 expression in the tumor tissue was significantly associated with high risk of recurrence following TUR-Bt. LncRNA contribution to self-renewal of bladder cancer stem-like cells (CSLCs) remains largely unknown. We investigated the expression profile and biological function of lncRNAs in urothelial CSLCs by microarray analysis. Among these, lncRNA-AK023096 was identified as potentially playing a role in maintaining self-renewal of CSLCs. Knockdown of this transcript inhibited spheroid formation and tumor formation. We found that AK023096 mediates recruitment of hnRNP-K to SOX2 promoter and increases H3K4 trimethylation status on SOX2 promoter, leading to a robust change in SOX2 mRNA and protein levels. Moreover, AK023096 expression in primary tumors was found to be a powerful predictor of recurrence following transurethral resection in patients with nonmuscle-invasive bladder cancer, highlighting the critical role of lncRNA in the bladder cancer regulatory network.

60 APPLIED LIFE SCIENCES↗