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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 73 records · Page 4

Development of a pilot system for converting sweet potato starch into glucose syrup

Sweet potato has been chosen as one of NASA's crops to support human beings in future space missions. One of the possible uses is to make syrup that can be used as a general sweetener. In this work a simple engineering system for converting sweet potato starch into glucose syrup was studied on a laboratory scale. The system comprises the following main units: a blender, continuous stirred tank reactor (CSTR), centrifugal and vacuum filters, deionization column and vacuum evaporator. The system was tested by carrying out conversion processes from fresh sweet potato roots. The roots were pealed, sliced, homogenized, heated and hydrolyzed by diastase of malt and Dextrozyme C (Novo Nordisk BioChem, North America, Inc.) enzymes in the CSTR. After hydrolysis the slurry was filtered, de-ionized and concentrated to get glucose syrup. The performance of the system was evaluated based on the quality of the conversion. The main factor was the level of reducing sugars except for the deionization where ash content and color were the main factors. Through careful control of the system units, good heating performance in the CSTR was obtained and the hydrolysis process attained sufficient conversion. The filtration process that incorporated the centrifuge was faster than when it was by-passed to the vacuum filter but losses in sugars were higher. Deionization removed more than 90% of the ash and reduced pigmentation, with probable insignificant losses in sugars during the deionization process. Recovery levels when the centrifuge was used and when it was by-passed could reach about 65% and 78%, respectively. These correspond to reducing sugar concentration of 259 and 310 mg/ml in 150-ml syrups from 300 g of sweet potatoes in each process. However, from concentration trials, syrups with volumes of 100 and 70 ml with the respective dextrose equivalence of 281 and 213 mg/ml were obtained. The syrups obtained were brownish in color and the process that employed centrifugal filtration gave a product with color that resembled the original color of the sweet potatoes. Further work is required to improve the overall system performance.

Non-NASA Center↗

Changes in soluble sugar, starch, and alcohol dehydrogenase in Arabidopsis thaliana exposed to N2 diluted atmospheres

Proper exchange of atmospheric gases is important for normal root and shoot metabolism in plants. This study was conducted to determine how restricted air supply affects foliar carbohydrates, while using the marker enzyme alcohol dehydrogenase (ADH) to report on the oxygenation status of the rootzone. Fourteen-day-old Arabidopsis thaliana (L.) Heynh. plants grown singly in 7-ml tubes containing agarified nutrient medium were placed in coupled Magenta vessels and exposed for six days to either ambient air or one of six different air/nitrogen dilutions. Redox potential of the agar medium was measured immediately after harvesting and freezing leaf tissue, and then root systems were quickly extracted from the agar and frozen for subsequent analyses. Redox potential measurements indicated that this series of gas mixtures produced a transition from hypoxia to anoxia in the root zones. Root ADH activity increased at higher rates as the redox potential neared anoxic levels. In contrast, ADH mRNA expression quickly neared its maximum as the medium became hypoxic and showed little further increase as it became anoxic. Foliar carbohydrate levels increased 1.5- to 2-fold with decreased availability of metabolic gases, with starch increasing at higher concentrations of air than soluble carbohydrate. The results serve as a model for plant performance under microgravity conditions, where absence of convective air movement prevents replenishment of metabolic gases.

NASA Discipline Plant Biology↗

Grasses with enhanced starch content

Methods and compositions for the production of monocot plants with increased starch content in stems are provided. In accordance with the invention, novel promoters and regulatory elements with specific temporal and spatial expression patterns are disclosed together with methods for the production of plants having desirable stem composition at harvest.

59 BASIC BIOLOGICAL SCIENCES↗

Effect of a novel starch-based temperature rise inhibitor on cement hydration and microstructure development: The second peak study

Temperature rise inhibitor (TRI) modifies the exothermic process of cement hydration at early age and mitigates the buildup of cumulative heat, which sheds new light on solving the thermal cracking issue. After the main hydration peak, a broader “second peak” appears and brings the cumulative heat back to normal level within 7 days (for additions less than 0.15%). This study shows that the “second peak” can be attributed, almost exclusively, to C{sub 3}S hydration while triggered by C{sub 3}A hydration. In a pure C{sub 3}S system, the heat flow is depressed by TRI during the main hydration peak but never recovers after. In a monophase system composed of C{sub 3}S, C{sub 3}A and gypsum or a real cement paste, the ongoing precipitation of ettringite helps to pump out TRI polymer from the pore solution and eliminate the restriction on C-S-H nucleation. Afterwards, the precipitation of C-S-H accelerates again to form the “second peak”.

36 MATERIALS SCIENCE↗

Modeling starch dynamics from seasonal variations of photosynthesis, growth and respiration

Abstract Nonstructural carbohydrates (NSCs) buffer differences in plant carbon supply (photosynthesis) and demand (respiration, growth, etc.), but the regulation of their dynamics remains unresolved. Seasonal variations in NSCs are well-documented, but differences in the time-average, amplitude, phase and other characteristics across ecosystems and functional types lack explanation; furthermore, observed dynamics do not always match expectations. The failure to match observed and expected dynamics has stimulated debate on whether carbon supply or demand drives NSC dynamics. To gain insight into how carbon supply and demand drive seasonal NSC dynamics, we derive a simple model of NSC dynamics based on carbon mass balance and linearizing the NSC demand to determine how supply-driven and demand-driven seasonal NSC dynamics differ. We find that supply-driven and demand-driven dynamics yield distinct timings of seasonal extrema, and supply overrides demand when carbon supply is low in winter (e.g., at high latitudes). Our results also suggest that NSC dynamics often lag changes carbon mass balance. We also predict differences in NSC dynamics across mass, suggesting that saplings are more dynamic and respond more quickly to the environment than mature trees. Our findings suggest that substrate-dependent regulation with environmental variation is sufficient to generate complex NSC dynamics.

Forestry↗

Starch Metabolism in Space-Grown Soybean Seedlings

The research conducted during this grant is described. There were three major areas of study: These were: (1) the interaction of gravity and sugar metabolism in soybean; (2) the effects of gravity on the photosynthetic activity of Brassica rapa; (3) investigation as to the effects of microgravity on the interaction of a fungal root pathogen with soybean.

Guikema, James A.↗

Starch Metabolism in Space-Grown Soybean Seedlings

The focus of this research was the study of sugar metabolism in soybean plants that had been in a clinorotation condition. The scope of activities was broadened greatly after the onset of the award. This broadening added two major research foci: 1) B-PAC: Photosynthetic activity of Brassica rapa; and 2) SOYPAT: The effects of microgravity on the interaction of a fungal root pathogen with soybean. Substantial investment and activity was also focused on the training of the astronaut team to conduct these experiments during orbital spaceflight.

Guikema, James A.↗