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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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62 records · Page 4
All sourcesNASA NTRSDOE OSTI
NASA NTRS2000

Over-Expression, Purification and Crystallization of Human Dihydrolipoamide Dehydrogenase

Dehydrolipoamide dehydrogenase (E3; dihydrolipoan-tide:NAD+ oxidoreductase, EC 1.8.1.4) is a common catalytic component found in pyruvate dehydrogenase complex, alpha-ketoglutarate dehydrogenase complex, and branched-chain cc-keto acid dehydrogenase complex. E3 is also a component (referred to as L protein) of the glycine cleavage system in bacterial metabolism (2). Active E3 forms a homodimer with four distinctive subdomain structures (FAD binding, NAD+ binding, central and interface domains) with non-covalently but tightly bound FAD in the holoenzyme. Deduced amino acids from cloned full-length human E3 gene showed a total of 509 amino acids with a leader sequence (N-terminal 35 amino acids) that is excised (mature form) during transportation of expressed E3 into mitochondria membrane. So far, three-dimensional structure of human E3 has not been reported. Our effort to achieve the elucidation of the X-ray crystal structure of human E3 will be presented. Recombinant pPROEX-1 expression vector (from GIBCO BRL Life Technologies) having the human E3 gene without leader sequence was constructed by Polymerase Chain Reaction (PCR) and subsequent ligation, and cloned in E.coli XL1-Blue by transformation. Since pPROEX-1 vector has an internal His-tag (six histidine peptide) located at the upstream region of a multicloning site, one-step affinity purification of E3 using nickelnitriloacetic acid (Ni-NTA) agarose resin, which has a strong affinity to His-tag, was feasible. Also a seven-amino-acid spacer peptide and a recombinant tobacco etch virus protease recognition site (seven amino acids peptide) found between His-tag and first amino acid of expressed E3 facilitated the cleavage of His-tag from E3 after the affinity purification. By IPTG induction, ca. 15 mg of human E3 (mature form) was obtained from 1L LB culture with overnight incubation at 25C. Over 98% of purity of E3 from one-step Ni-NTA agarose affinity purification was confirmed by SDS-PAGE analysis. For crystallization, E3 samples were prepared with and without His-tag. To minimize the aggregation of E3, apo- and holo- forms of E3s were tested, as well as a mutated E3. Dynamic light scattering measurements revealed that the E3 preparations without His-tag and substrate are highly monodispersive with regard to homodimers. Consequent crystallization trials of this E3 preparation led to single crystals of E3 grown by the vapor diffusion method. Crystals were obtained within a few days from solution containing poly (ethylene glycol) monomethyl ether 5000 as a precipitant. Autoindexing and integration of the X-ray diffraction data showed that E3 crystals belong to an orthorhombic system with unit cell parameters a-- 123. 1, b= 165.3 and c=214.3A. Further optimization of protein preparation and crystallization experiments for the structural determination will be discussed.

Hong, Y. S.↗
NASA NTRS1985

The propagation of solar flare particles in a turbulent coronal loop

Energetic solar flare particles, both electrons and protons, must survive the turbulent environment of a flaring loop and propogate to the lower corona or chromosphere in order to produce hard X-ray and gamma ray bursts. This plasma turbulence, often observed in soft X-ray line widths to be in excess of 100 km/s, is presumably capable of efficiently scattering the fast flare particles. This prevents to some degree the free streaming of accelerated particles and depending on the amplitude of the turbulence, restricts the particles to diffusive propagation along the length of the loop to the target chromosphere. In addition this turbulence is capable of performing additional acceleration of the fast particles by the second order Fermi mechanism. For compact flares with rise times 10s, the acceleration effect is small and the propagation of the particles is governed by spatial diffusion and energy loss in the ambient medium. The solution of the time dependent diffusion equation with velocity dependent diffusion and energy loss coefficients yields for the case of nonrelativistic protons particle precipitation rates which are necessary for calculating thick target gamma ray emission and also yields the total thin target emissivity.

Ryan, J. M.↗
NASA NTRS1984

Variation of the pulse profile of Hercules X-1

The X-ray pulsar Her X-1 was observed in an on-state during its 35th cycle of activity in May, 1983 using the gas scintillation proportional counter (GSPC) array of the Tenma X-ray astronomy satellite. The outstanding features observed during the declining phase of the on-state included: a sharp decrease in the main X-ray pulse amplitude; and a steady increase in the column density of cool matter. On the basis of the spectral shape of the pulses, it is suggested that the main phase was attenuated due to electron scattering of the X-ray beam in a highly ionized medium located 3 x 10 to the 8th cm from the neutron star. Near the end of the on-state, the main pulse totally disappeared and a plain sinusoidal profile was observed. The observed pulse profiles are reproduced in graphic form.

Ohashi, T.↗
NASA NTRS1993

Radiation from magnetized accretion disks in active galactic nuclei

We present a model of AGN based on accretion disks around 10 exp 8 solar mass black holes, which incorporates a strong magnetic field in the disk, with loops above the disk in which B is about 100 G. The magnetic energy in the loops dissipates, accelerating electrons to relativistic energies. The electrons emit synchrotron and inverse-Compton radiation, much of which is reflected or scattered by gas in the disk. We calculate the emitted spectrum from infrared to gamma rays. Of the total emission, 23 percent goes into a nu exp -1 power law and 77 percent into thermal radiation at 24,000 K (due to the disk, which is heated by nonthermal radiation and energetic ions). The model accounts quantitatively for the X-ray and gamma-ray backgrounds, and it accounts qualitatively for certain features observed in individual AGNs: a far-infrared cutoff, a minimum at a few microns, the ultraviolet bump, the Compton reflection bump at about 30 keV, and a gamma-ray tail.

Field, G. B.↗
NASA NTRS1997

Particle Acceleration in Active Galactic Nuclei

The high efficiency of energy generation inferred from radio observations of quasars and X-ray observations of Seyfert active galactic nuclei (AGNs) is apparently achieved only by the gravitational conversion of the rest mass energy of accreting matter onto supermassive black holes. Evidence for the acceleration of particles to high energies by a central engine is also inferred from observations of apparent superluminal motion in flat spectrum, core-dominated radio sources. This phenomenon is widely attributed to the ejection of relativistic bulk plasma from the nuclei of active galaxies, and accounts for the existence of large scale radio jets and lobes at large distances from the central regions of radio galaxies. Reports of radio jets and superluminal motion from galactic black hole candidate X-ray sources indicate that similar processes are operating in these sources. Observations of luminous, rapidly variable high-energy radiation from active galactic nuclei (AGNs) with the Compton Gamma Ray Observatory show directly that particles are accelerated to high energies in a compact environment. The mechanisms which transform the gravitational potential energy of the infalling matter into nonthermal particle energy in galactic black hole candidates and AGNs are not conclusively identified, although several have been proposed. These include direct acceleration by static electric fields (resulting from, for example, magnetic reconnection), shock acceleration, and energy extraction from the rotational energy of Kerr black holes. The dominant acceleration mechanism(s) operating in the black hole environment can only be determined, of course, by a comparison of model predictions with observations. The purpose of the work proposed for this grant was to investigate stochastic particle acceleration through resonant interactions with plasma waves that populate the magnetosphere surrounding an accreting black hole. Stochastic acceleration has been successfully applied to the problem of ion and electron energization in solar flares, and is capable of accounting for a wide range of both neutral and charged particle emissions. It is also a component in diffusive shock acceleration, since pitch-angle scattering (which is necessary for multiple shock crossings) is accompanied by diffusion in momentum space, which in turn yields a net systematic energy gain; however, stochastic energization will dominate the first-order shock process only in certain parameter regimes. Although stochastic acceleration has been applied to particle energization in the lobes of radio galaxies, its application to the central regions of AGNs has only recently been considered, but not in detail. We proposed to systematically investigate the plasma processes responsible for stochastic particle acceleration in black hole magnetospheres along with the energy-loss processes which impede particle energization. To this end we calculated acceleration rates and escape time scales for protons and electrons resonating with Alfven waves, and for electrons resonating with whistlers. Assuming either a Kolmogorov or Kraichnan wave spectrum, accretion at the Eddington limit, magnetic field strengths near equipartition, and turbulence energy densities approx. 10% of the total magnetic field energy density, we find that Alfven waves accelerate protons to Lorentz factors approx, equals 10(exp 4) - 10(exp 6) before they escape from the system. Acceleration of electrons by fast mode and whistler waves can produce a nonthermal population of relativistic electrons whose maximum energy is determined by a competition with radiation losses.

Miller, James A.↗
NASA NTRS2003

A New Method to Resolve X-Ray Halos around Point Sources with Chandra Data and Its Application to Cygnus X-1

With excellent angular resolution, good energy resolution, and a broad energy band, the Chandra Advanced CCD Imaging Spectrometer (ACIS) is the best instrument for studying the X-ray halos around some Galactic X-ray point sources caused by the dust scattering of X-rays in the interstellar medium. However, the direct images of bright sources obtained with the ACIS usually suffer from severe pileup. Making use of the fact that an isotropic image could be reconstructed from its projection in to any direction, we can reconstruct the images of the X-ray halos from the data obtained with the High Energy Transition Grating Spectrometer (HETGS) and/or in continuos clocking (CC) mode. These data have no or less serious pileup and enable us to take full advantage of the excellent angular resolution of Chandra. With the reconstructed high-resolution images, we can probe the X-ray halos as close as 1" to their associated point sources. Applying this method to Cygnus X-1 observed with the Chandra HETGS in CC mode, we derived an energy-dependent radial halo flux distribution and concluded that in a circular region (2' in radius) centered a the point source: (1) relative to the total intensity, the fractional halo intensity is about 15% at keV and drops to aboout 5% at approximately 6 keV (2) about 50% of the halo photons are within the region of a radius less than 40 inches and (3) the spectrum of the pooint source is slightly distorted by the halo contamination.

Yao, Yangsen↗
NASA NTRS2011

The Nature and Cause of Spectral Variability in LMC X-1

We present the results of a long-term observation campaign of the extragalactic wind-accreting black-hole X-ray binary LMC X-1, using the Proportional Counter Array on the Rossi X-Ray Timing Explorer (RXTE). The observations show that LMC X-1's accretion disk exhibits an anomalous temperature-luminosity relation. We use deep archival RXTE observations to show that large movements across the temperature-luminosity space occupied by the system can take place on time scales as short as half an hour. These changes cannot be adequately explained by perturbations that propagate from the outer disk on a viscous timescale. We propose instead that the apparent disk variations reflect rapid fluctuations within the Compton up-scattering coronal material, which occults the inner parts of the disk. The expected relationship between the observed disk luminosity and apparent disk temperature derived from the variable occultation model is quantitatively shown to be in good agreement with the observations. Two other observations support this picture: an inverse correlation between the flux in the power-law spectral component and the fitted inner disk temperature, and a near-constant total photon flux, suggesting that the inner disk is not ejected when a lower temperature is observed.

Ruhlen, L.↗
NASA NTRS2020

Inverse Compton Cooling in the Coronae of Simulated Black Hole Accretion Flows

We present a formulation for a local cooling function to be employed in the diffuse, hot corona region of 3D GRMHD simulations of accreting black holes. This new cooling function calculates the cooling rate due to inverse Compton scattering by considering the relevant microphysics in each cell in the corona and approximating the radiation energy density and Compton temperature there by integrating over the thermal seed photon flux from the disk surface. The method either assumes ion and electron tem- peratures are equal (1T), or calculates them separately (2T) using an instantaneous equilibrium approach predicated on the actual relevant rate equations (Coulomb and Compton). The method is shown to be consistent with a more detailed ray-tracing calculation where the bulk of the cooling occurs, but is substantially less costly to per- form. As an example, we apply these methods to a harm3d simulation of a 10M⊙, non-spinning black hole, accreting at nominally 1% the Eddington value. Both 1T and 2T approaches lead to increased radiative efficiency and a larger fraction of total cooling in the corona as compared to the original target-temperature cooling function used by harm3d, especially in the 1T case. Time-averaged post-processing reveals that the continuum spectral observations predicted from these simulations are qualitatively similar to actual X-ray binary data, especially so for the 1T approach which yields a harder power-law component (Γ = 2.25) compared to the 2T version (Γ = 2.53).

Brooks E. Kinch↗