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Model of metabolism and gene expression predicts proteome allocation in Pseudomonas putida

Abstract The genome-scale model of metabolism and gene expression (ME-model) forPseudomonas putidaKT2440,iPpu1676-ME, provides a comprehensive representation of biosynthetic costs and proteome allocation. Compared to a metabolic-only model,iPpu1676-ME significantly expands on gene expression, macromolecular assembly, and cofactor utilization, enabling accurate growth predictions without additional constraints. Multi-omics analysis using RNA sequencing and ribosomal profiling data revealed translational prioritization inP. putida, with core pathways, such as nicotinamide biosynthesis and queuosine metabolism, exhibiting higher translational efficiency, while secondary pathways displayed lower priority. Notably, the ME-model significantly outperformed the M-model in alignment with multi-omics data, thereby validating its predictive capacity. Thus,iPpu1676-ME offers valuable insights intoP. putida’s proteome allocation and presents a powerful tool for understanding resource allocation in this industrially relevant microorganism.

Mathematical & Computational Biology

Forest Age Rivals Climate to Explain Reproductive Allocation Patterns in Forest Ecosystems Globally

ABSTRACT Forest allocation of net primary productivity (NPP) to reproduction (carbon required for flowers, fruits, and seeds) is poorly quantified globally, despite its critical role in forest regeneration and a well‐supported trade‐off with allocation to growth. Here, we present the first global synthesis of a biometric proxy for forest reproductive allocation (RA) across environmental and stand age gradients from a compiled dataset of 824 observations across 393 sites. We find that ecosystem‐scale RA increases ~60% from boreal to tropical forests. Climate shows important non‐linear relationships with RA, but is not the sole predictor. Forest age effects are comparable to climate in magnitude (MAT: ß = 0.24,p = 0.021; old growth forest: ß = 0.22,p < 0.001), while metrics of soil fertility show small but significant relationships with RA (soil pH: ß = 0.07,p = 0.001; soil N: ß = −0.07,p = 0.001). These results provide strong evidence that ecosystem‐scale RA is mediated by climate, forest age, and soil conditions, and is not a globally fixed fraction of positive NPP as assumed by most vegetation and ecosystem models. Our dataset and findings can be used by modellers to improve predictions of forest regeneration and carbon cycling.

Environmental Sciences & Ecology

Transmission Cost Allocation Practices

This technical brief summarizes approaches to allocating costs of transmission facilities between generators and loads, jurisdictions, and customer classes, and based on project drivers. The brief also discusses planning approaches, stakeholder engagement, considerations for using counterfactuals for cost allocation, and Federal Energy Regulatory Commission and court decisions on cost allocation proposals.

24 POWER TRANSMISSION AND DISTRIBUTION

Optimum allocation of redundancy among subsystems connected in series

This analysis considers the optimum allocation of redundancy in a system of serially connected subsystems in which each subsystem is of the k-out-of-n type. Redundancy is optimally allocated when: (1) reliability is maximized for given costs; or (2) costs are minimized for given reliability. Several techniques are presented for achieving optimum allocation and their relative merits are discussed. Approximate solutions in closed form were attainable only for the special case of series-parallel systems and the efficacy of these approximations is discussed.

Bien, D. D.

Spacelab task allocation - A preliminary determination of onboard versus ground operational priorities

A three-phase approach was developed for allocating specific mission tasks to the onboard crew or the ground support team in the Spacelab project. Phase one involved a list of potential operational tasks anticipated for Spacelab missions, phase two, the development of a set of criteria for allocating task-performance location, phase three, the combination of these criteria to allow tasks to be arranged in order of priority. The task-allocation criteria included crew safety, work load, cost, efficiency, weight/space, accessibility, complexity, response time, and capability.

Harris, W. J.

Linear modelling of attentional resource allocation

Eight subjects time-shared performance of two compensatory tracking tasks under conditions when both were of constant difficulty, and when the control order of one task (designated primary) was varied over time within a trial. On line performance feedback was presented on half of the trials. The data are interpreted in terms of a linear model of the operator's attention allocation system, and suggest that this allocation is strongly suboptimal. Furthermore, the limitations in reallocating attentional resources between tasks, in response to difficulty fluctuations were not reduced by augmented performance feedback. Some characteristics of the allocation system are described, and reasons for its limitations suggested.

Pierce, B.

Manpower Allocation and Reporting

Interactive Manpower Allocation and Reporting System (MARS) helps planners make manpower allocation decisions. Includes provisions to enter overall constraints for projects and persons, assign individuals to projects, compute automatically overhead category, report on deviations from constraints, and generate manpower resource allocation reports.

Merwarth, P. D.

Ka-band (32 GHz) allocations for deep space

At the 1979 World Administrative Conference, two new bands were allocated for deep space telecommunications: 31.8 to 32.3 GHz, space-to-Earth, and 34.2 to 34.7 GHz, Earth-to-space. These bands provide opportunity for further development of the Deep Space Network and its support of deep space research. The history of the process by which JPL/NASA developed the rationale, technical background, and statement of requirement for the bands are discussed. Based on this work, United States proposals to the conference included the bands, and subsequent U.S. and NASA participation in the conference led to successful allocations for deep space telecommunications in the 30 GHz region of the spectrum. A detailed description of the allocations is included.

Degroot, N. F.

Space Network Control Conference on Resource Allocation Concepts and Approaches

The results are presented of the Space Network Control (SNC) Conference. In the late 1990s, when the Advanced Tracking and Data Relay Satellite System is operational, Space Network communication services will be supported and controlled by the SNC. The goals of the conference were to survey existing resource allocation concepts and approaches, to identify solutions applicable to the Space Network, and to identify avenues of study in support of the SNC development. The conference was divided into three sessions: (1) Concepts for Space Network Allocation; (2) SNC and User Payload Operations Control Center (POCC) Human-Computer Interface Concepts; and (3) Resource Allocation Tools, Technology, and Algorithms. Key recommendations addressed approaches to achieving higher levels of automation in the scheduling process.

Moe, Karen L.

Space Network Control (SNC) Conference on Resource Allocation Concepts and Approaches. Overview

In session 1 of the conference, Concepts for space network resource allocation was the main topic. In session 2, Space Network Control and user payload operations and control center human-computer interface, was the topic of discussion. The topic of session 3 was Resource allocation tools, technology, and algorithms. Some of the stated goals for the conference are as follows: to survey existing resource allocation concepts and approaches; to identify solutions applicable to the SN problem; to identify fruitful avenues of study in support of SNC development; and to capture knowledge in proceedings and make available to bidders on the SNC concept definition procurement.

Source record

An exploration of function analysis and function allocation in the commercial flight domain

The applicability is explored of functional analysis methods to support cockpit design. Specifically, alternative techniques are studied for ensuring an effective division of responsibility between the flight crew and automation. A functional decomposition is performed of the commercial flight domain to provide the information necessary to support allocation decisions and demonstrate methodology for allocating functions to flight crew or to automation. The function analysis employed 'bottom up' and 'top down' analyses and demonstrated the comparability of identified functions, using the 'lift off' segment of the 'take off' phase as a test case. The normal flight mission and selected contingencies were addressed. Two alternative methods for using the functional description in the allocation of functions between man and machine were investigated. The two methods were compared in order to ascertain their relative strengths and weaknesses. Finally, conclusions were drawn regarding the practical utility of function analysis methods.

Mcguire, James C.

Automated system function allocation and display format: Task information processing requirements

An important consideration when designing the interface to an intelligent system concerns function allocation between the system and the user. The display of information could be held constant, or 'fixed', leaving the user with the task of searching through all of the available information, integrating it, and classifying the data into a known system state. On the other hand, the system, based on its own intelligent diagnosis, could display only relevant information in order to reduce the user's search set. The user would still be left the task of perceiving and integrating the data and classifying it into the appropriate system state. Finally, the system could display the patterns of data. In this scenario, the task of integrating the data is carried out by the system, and the user's information processing load is reduced, leaving only the tasks of perception and classification of the patterns of data. Humans are especially adept at this form of display processing. Although others have examined the relative effectiveness of alphanumeric and graphical display formats, it is interesting to reexamine this issue together with the function allocation problem. Currently, Johnson Space Center is the test site for an intelligent Thermal Control System (TCS), TEXSYS, being tested for use with Space Station Freedom. Expert TCS engineers, as well as novices, were asked to classify several displays of TEXSYS data into various system states (including nominal and anomalous states). Three different display formats were used: fixed, subset, and graphical. The hypothesis tested was that the graphical displays would provide for fewer errors and faster classification times by both experts and novices, regardless of the kind of system state represented within the display. The subset displays were hypothesized to be the second most effective display format/function allocation condition, based on the fact that the search set is reduced in these displays. Both the subset and the graphic display conditions were hypothesized to be processed more efficiently than the fixed display conditions.

Czerwinski, Mary P.

Method for optimizing resource allocation in a government organization

The managers in Federal agencies are challenged to control the extensive activities in government and still provide high-quality products and services to the American taxpayers. Considering today's complex social and economic environment and the $3.8 billion daily cost of operating the Federal Government, it is evident that there is a need to develop decision-making tools for accurate resource allocation and total quality management. The goal of this thesis is to provide a methodical process that will aid managers in Federal Government to make budgetary decisions based on the cost of services, the agency's objectives, and the customers' perception of the agency's product. A general resource allocation procedure was developed in this study that can be applied to any government organization. A government organization, hereafter the 'organization,' is assumed to be a multidivision enterprise. This procedure was applied to a small organization for the proof of the concept. This organization is the Technical Services Directorate (TSD) at the NASA Lewis Research Center in Cleveland, Ohio. As part of the procedure, a nonlinear programming model was developed to account for the resources of the organization, the outputs produced by the organization, the decision-maker's views, and the customers' satisfaction with the organization. The information on the resources of the organization was acquired from current budget levels of the organization and the human resources assigned to the divisions. The outputs of the organization were defined and measured by identifying metrics that assess the outputs, the most challenging task in this study. The decision-maker's views are represented in the model as weights assigned to the various outputs and were quantified by using the analytic hierarchy process. The customer's opinions regarding the outputs of the organization were collected through questionnaires that were designed for each division individually. Following the philosophy of total quality management, information on customers' satisfaction is presented in the model as the quality of output. The model is a nonlinear one whose objective is to maximize customers' satisfaction such that the total cost of operation does not exceed the organization's budget. This model represents a structured approach or policy mechanism, at the agency level, to make capital investment decisions based on the priorities of the agency and the quality of outputs. This procedure applied to TSD resulted in a resources allocation scheme that was reasonable and acceptable to the decision-makers and, as expected, dependent on the assumptions and accuracy of the data used in the model.

Afarin, James

A model for analysis of TDA budget allocation

There is an ever-increasing need to achieve greater efficiency in the operation of the Deep Space Network (DSN), i.e., increased productivity at reduced cost. One of the tools used in the course of a planning workshop on this subject was a methodology for budget allocation applicable to long-range planning. This article presents a model for analysis of the TDA budget allocation. For the 1994 through 1999 period, the percentage of the total TDA budget allocated to capacity and capability is being cut in half, whereas the percentage spent on efficiency of delivery will be increasing.

Remer, D. S.

Neural Network Based Modeling and Analysis of LP Control Surface Allocation

This paper presents an approach to interpretive modeling of LP based control allocation in intelligent flight control. The emphasis is placed on a nonlinear interpretation of the LP allocation process as a static map to support analytical study of the resulting closed loop system, albeit in approximate form. The approach makes use of a bi-layer neural network to capture the essential functioning of the LP allocation process. It is further shown via Lyapunov based analysis that under certain relatively mild conditions the resulting closed loop system is stable. Some preliminary conclusions from a study at Ames are stated and directions for further research are given at the conclusion of the paper.

Langari, Reza

Auction Mechanism to Allocate Air Traffic Control Slots

This article deals with an auction mechanism for airspace slots, as a means of solving the European airspace congestion problem. A disequilibrium, between Air Traffic Control (ATC) services supply and ATC services demand are at the origin of almost one fourth of delays in the air transport industry in Europe. In order to tackle this congestion problem, we suggest modifying both pricing and allocation of ATC services, by setting up an auction mechanism. Objects of the auction will be the right for airlines to cross a part of the airspace, and then to benefit from ATC services over a period corresponding to the necessary time for the crossing. Allocation and payment rules have to be defined according to the objectives of this auction. The auctioneer is the public authority in charge of ATC services, whose aim is to obtain an efficient allocation. Therefore, the social value will be maximized. Another objective is to internalize congestion costs. To that end, we apply the principle of Clarke-Groves mechanism auction: each winner has to pay the externalities imposed on other bidders. The complex context of ATC leads to a specific design for this auction.

Raffarin, Marianne

Optimal Sensor Allocation for Fault Detection and Isolation

Automatic fault diagnostic schemes rely on various types of sensors (e.g., temperature, pressure, vibration, etc) to measure the system parameters. Efficacy of a diagnostic scheme is largely dependent on the amount and quality of information available from these sensors. The reliability of sensors, as well as the weight, volume, power, and cost constraints, often makes it impractical to monitor a large number of system parameters. An optimized sensor allocation that maximizes the fault diagnosibility, subject to specified weight, volume, power, and cost constraints is required. Use of optimal sensor allocation strategies during the design phase can ensure better diagnostics at a reduced cost for a system incorporating a high degree of built-in testing. In this paper, we propose an approach that employs multiple fault diagnosis (MFD) and optimization techniques for optimal sensor placement for fault detection and isolation (FDI) in complex systems. Keywords: sensor allocation, multiple fault diagnosis, Lagrangian relaxation, approximate belief revision, multidimensional knapsack problem.

Azam, Mohammad

Frequency Allocations for Unmanned Aircraft Systems in the National Airspace. Access 5 White Paper to the WRC Advisory Committee

A critical aspect of the Access 5 program is identifying appropriate spectrum for civil and commercial purposes. However, currently, there is no spectrum allocated for the command/control link between the aircraft control station and the unmanned aircraft. Until such frequency spectrum is allocated and approved, it will be difficult for the UAS community to obtain civil airworthiness certification and operate in the NAS on a routine basis. This document provides a perspective from the UAS community on Agenda Items being considered for the upcoming World Radiocommunication Conference 2007 (WRC 07). Primarily, it supports the proposal to add Aeronautical Mobile (Route) Services (AM(R)S) to existing bands that could be used for UAS Line-of-Sight operations. It also recommends the need to identify spectrum that could be used for an Aeronautical Mobile Satellite (Route) Service (AMS(R)S) that would allow UAS to operate Beyond Line-of-Sight. If spectrum is made available to provide these services, it will then be incumbent upon the UAS community to justify their use of this spectrum as well as the assurance that they will not interfere with other users of this newly allocated spectrum.

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