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Optical imaging systems achieving aperture synthesis by lensless Fourier transform holography, noting X ray astronomy, ultrasonic and space applications
A method is described for performing the synthesis and analysis of digital color images. The method is based on two principles. First, image data are represented with respect to the separate physical factors, surface reflectance and the spectral power distribution of the ambient light, that give rise to the perceived color of an object. Second, the encoding is made efficient by using a basis expansion for the surface spectral reflectance and spectral power distribution of the ambient light that takes advantage of the high degree of correlation across the visible wavelengths normally found in such functions. Within this framework, the same basic methods can be used to synthesize image data for color display monitors and printed materials, and to analyze image data into estimates of the spectral power distribution and surface spectral reflectances. The method can be applied to a variety of tasks. Examples of applications include the color balancing of color images, and the identification of material surface spectral reflectance when the lighting cannot be completely controlled.
A method is described for performing the synthesis and analysis of digital color images. The method is based on two principles. First, image data are represented with respect to the separate physical factors, surface reflectance and the spectral power distribution of the ambient light, that give rise to the perceived color of an object. Second, the encoding is made efficiently by using a basis expansion for the surface spectral reflectance and spectral power distribution of the ambient light that takes advantage of the high degree of correlation across the visible wavelengths normally found in such functions. Within this framework, the same basic methods can be used to synthesize image data for color display monitors and printed materials, and to analyze image data into estimates of the spectral power distribution and surface spectral reflectances. The method can be applied to a variety of tasks. Examples of applications include the color balancing of color images, and the identification of material surface spectral reflectance when the lighting cannot be completely controlled.
Fatty acids can be produced biosynthetically in microbes and these compounds can serve as precursors to biodiesels and other high value oleochemicals. However, progress on engineering fatty acid biosynthesis, and biofuel synthesis more generally, has been hindered by current quantification methods that are either indirect or not amenable to high-throughput or single-cell resolution screening. In this project, we assembled an interdisciplinary team with complimentary expertise in synthetic biology and microscopy, metabolic engineering, and chemical imaging to address these challenges. We used chemical imaging to directly measure lipid biosynthesis in Escherichia coli engineered to produce fatty acids, obtaining detailed single-cell resolution measurements. We deployed stimulated Raman scattering (SRS) microscopy in concert with multiplexed genome engineering and gene circuit design strategies from synthetic biology to optimize production of fatty acids. These results provided novel insight into cell-to-cell heterogeneity present in biofuel production strains. In addition, we introduced new chemical imaging methods which are label-free and do not require fluorescent reporters. These efforts were complemented by other studied developing foundational tools for regulation and control, which offer excellent potential for advancing researchers’ ability to rapidly design, build, and test strains for enhanced biofuel synthesis.
The Band Width Synthesis (BWS) delay and delay rate observables measured by VLBI are used in astrometry to determine positions of extragalactic radio sources with a precision of 0.001 arcsec. At this level of precision, extragalactic radio sources exhibit extended structures that induce significant effects in the measured BWS delays and delay rates. The question whether closure BWS delays and delay rates could supplement the amplitudes and closure phases for VLBI imaging is studied. Preliminary results for the radio source NRAO140 observed in July 1983 are presented.
A new approach is reported to the measurement of the complete polarization signature of each resolution element in an image implemented with an airborne synthetic aperture radar system. Signals recorded on one data pass from orthogonal linearly polarized antennas are utilized. The signals are combined in a data processor to synthesize any desired combination of transmit and receive polarizations. The technique permits measurement of the complex, multichannel reflectivity of a scene on a single aircraft pass and to late reprocess the data to provide multiple image maps, with each representing the backscattered energy from the scene measured with a different combination of observational transmit and recieve polarizations. The resulting polarization signature measurements indicate optimum polarizations for observations of certain classes of objects and give insight into the identification of dominant scattering mechanisms for each kind of object. The mathematical model for polarization synthesis is summarized, and some theoretical polarization measurements are illustrated for several types of targets. The overall radar system implementation is described in detail. Some analyses of data acquired on three aircraft flights are presented. The technique has been applied to mapping and differentiation of lava flows and to differentiation of forested and clear-cut areas.
No abstract available
Interactive program yields eigenvectors, eigenvalues, and gains for feedback-control systems. Interactive Modal Analysis and Gain Estimation System (IMAGES) provides eigensystem synthesis capability to control-system engineer. IMAGES modular and flexible. Capable of both modal and spectral synthesis of multi-input control systems. IMAGES user-oriented, interactive program that frees engineer to concentrate on eigensystem synthesis. Engineer provided with scratch-pad capability that speeds control-system design.
It is shown that it is possible to measure the complete scattering matrix of an object using data acquired on a single aircraft pass, and combine the signals later in the data processor to generate radar images corresponding to any desired combination of transmit and receive polarization. Various scattering models predict different dependence on polarization state of received power from an object. The imaging polarimeter permits determination of this dependence, the polarization signature, of each point in a radar image. Comparison of predictions and observational data reveals scattering mechanisms for each area of interest. Backscatter from the ocean is highly polarized and well-modeled by Bragg scattering, while scattering from trees in a city park possesses a considerable unpolarized component. Urban regions exhibit the characteristics expected from dihedral corner reflectors, and their polarization signatures are quite different from the on-bounce Bragg model.
It was shown that it is possible to measure the complete scattering matrix of an object using data acquired on a single aircraft pass, and can combine the signals later in the data processor to generate radar images corresponding to any desired combination of transmit and receive polarization. Various scattering models predict different dependence on polarization state of received power from an object. The imaging polarimeter permits determination of this dependence, which is called the polarization signature, of each point in a radar image. Comparison of the theoretical predictions and observational data yield identification of possible scattering mechanisms for each area of interest. It was found that backscatter from the ocean is highly polarized and well-modeled by Bragg scattering, while scattering from trees in a city park possesses a considerable unpolarized component. Urban regions exhibit the characteristics expected from dihedral corner reflectors and their polarization signatures are quite different from the one-bounce Bragg model.
Major improvements currently underway to the Arecibo 305 m antenna and 13 cm wavelength radar system will currently increase the radar system's sensitivity by a factor of about 20. Recent upgrades to the Goldstone 3.5 cm wavelength radar system have also improved its sensitivity. While the Arecibo system will have significantly greater sensitivity than Goldstone, the Arecibo antenna's limited declination coverage of -2(deg) to +38(deg) means that the Goldstone system will be important for the investigation of near-Earth objects with declinations outside this range. Over one hundred mainbelt and near-Earth asteroids per year will be observable with the new Arecibo system, and there will be a number of good imaging opportunities each year. While only a few imaging opportunities are predicted for comets over the next 10 years, the system will be able to respond to unexpected cometary opportunities. We are currently investigating the applicability of VLBI techniques to the direct synthesis of images of asteroids and comets illuminated by a radar signal.
The innate immune system serves as the body’s first line of defense against invading pathogens, responding rapidly through the deployment of immune cells at common sites of infection, such as the skin and airways. These immune-cell sentinels express a range of highly conserved receptors, including Toll-like receptors (TLRs), which recognize and bind to pathogen-derived components. This recognition event triggers intracellular signaling cascades that initiate and coordinate immune responses. Despite their significance, the complete structural characterization of full-length TLRs, including their extracellular domain (ECD), transmembrane domain (TMD), and Toll/interleukin-1 receptor (TIR) domain, remains incomplete. In this study, we examined the expression and isolation of human TLR4 incorporated into nanodiscs using two approaches: a cell-free synthesis system and a cell-based transfection strategy employing Expi293F cells derived from the human embryonic kidney lineage. Our findings demonstrate that NLP-bound human TLR4 produced via the cell-based method yielded functional protein suitable for time-resolved single-particle cryo-electron microscopy (cryo-EM). This advancement enables structural characterization of full-length TLR4, maintaining the integrity of its extracellular domain, transmembrane domain, and Toll/Interleukin-1 receptor (TIR) domain.
Ka-band (32 GHz, 9mm) Very Long Baseline Interferometric (VLBI) networking has now begun and has tremendous potential for expansion over the next few years. Ka-band VLBI astrometry from NASA's Deep Space Network has already developed a catalog of 470 observable sources with highly accurate positions. Now, several antennas worldwide are planning or are considering adding Ka-band VLBI capability. Thus, there is now an opportunity to create a worldwide Ka-band network with potential for high resolution imaging and astrometry. With baselines approaching a Giga-lambda, a Ka-band network would be able to probe source structure at the nano-radian (200 as) level ( 100X better than Hubble) and thus gain insight into the astrophysics of the most compact regions of emission in active galactic nuclei. We discuss the advantages of Ka-band, show the known sources and candidates, simulate projected baseline (uv) coverage, and discuss potential radio frequency feeds. The combination of these elements demonstrates the feasibility of a worldwide Ka network within the next few years!
The Space Systems Integration and Operations Research Applications (SIORA) Program was initiated in late 1986 as a cooperative applications research effort between Stanford University, NASA Kennedy Space Center, and Lockheed Space Operations Company. One of the major initial SIORA tasks was the application of automation and robotics technology to all aspects of the Shuttle tile processing and inspection system. This effort has adopted a systems engineering approach consisting of an integrated set of rapid prototyping testbeds in which a government/university/industry team of users, technologists, and engineers test and evaluate new concepts and technologies within the operational world of Shuttle. These integrated testbeds include speech recognition and synthesis, laser imaging inspection systems, distributed Ada programming environments, distributed relational database architectures, distributed computer network architectures, multimedia workbenches, and human factors considerations.
The possibility of using aperture synthesis to image other planetary systems at submillimeter wavelengths is considered. The approach requires only a small increase in dynamic range over that already demonstrated by the VLA at longer wavelengths. The values of the relevant parameters are calculated and presented.
The Tile Automation System includes applications of automation and robotics technology to all aspects of the Shuttle tile processing and inspection system. An integrated set of rapid prototyping testbeds was developed which include speech recognition and synthesis, laser imaging systems, distributed Ada programming environments, distributed relational data base architectures, distributed computer network architectures, multi-media workbenches, and human factors considerations. Remote voice training in the Tile Automation System is discussed. The user is prompted over a headset by synthesized speech for the training sequences. The voice recognition units and the voice output units are remote from the user and are connected by Ethernet to the main computer system. A supervisory channel is used to monitor the training sequences. Discussions include the training approaches as well as the human factors problems and solutions for this system utilizing remote training techniques.