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At least 73 records · Page 4

Formation mechanisms of laboratory double layers

The evolution processes of double layers were studied in a series of laboratory experiments. It was found that the existence of virtual cathode-type potential wells at the electron injection boundary was the dominant triggering mechanism. The rapid growth of the potential wells led to collisionless ion trapping and the establishment of the necessary trapped ion population. For double layers with small potential drops, collisionless ion trapping actually induced ion-ion streaming instabilities and the formation of ion phase-space vortices. In this regime, the system often exhibited relaxation-type oscillations which correspond to the disruption and the recovery of the double layers.

Chan, Chung

Studying Microbial Adaptation in the Laboratory: Sensor & Control Upgrades for an Experimental Evolution Biofluidics System

Experimental evolution (EE) involves iteratively exposing a microbial community to specific stressors to study its response to changes in environment over time. EE work is commonly done manually in the laboratory, but, when there are many environmental variables to measure and adjust, it is highly labor intensive, prone to human error, and challenging to scale. Single-purpose automated continuous culturing chambers exist, but implement only limited stressor types. A more general-purpose design is desirable. The BeING Lab at Ames Research Center created the prototype Automated Adaptive Directed Evolution Chamber (AADEC) to address these problems, beginning with Escherichia coli tolerance of short-wave ultraviolet (UV-C) radiation and of temperature. In newer versions, AADEC monitors microbial activity and can adjust the UV-C and temperature levels automatically. An optical density measurement is used to determine how many cells are present in the growth medium—over time, this corresponds to how many survive and reproduce. Oxidation-reduction potential provides information on consumed metabolic energy, and pH and electrical conductivity on metabolic products. Dissolved oxygen content is used to determine aerobic vs anaerobic growth. A Raspberry Pi computer processes all this data to set the UV-C stressor level. AADEC’s auxiliary systems include peristaltic pumps to change media and agitation to counteract cell settling. These actuators can also act as additional stressors. With the Raspberry Pi monitoring sensors and adjusting actuators in real time, AADEC takes measurements and controls the environment much more accurately than can be done with a manual EE implementation. The third and latest AADEC iteration is the first to simplify design and usage with circuits on PCBs and the ability to pre-program experimental protocols. Still planned is expansion to a multi-well design for the study of varying cell cultures in parallel, which will enable researchers to retain and re-inoculate cultures exhibiting the desired trait most strongly while flushing out others. AADEC’s special capabilities make it a valuable tool for studying life under multiple stressors, enabling scientists to replicate changes in climate on microbes for study in a lab setting.

Microbial Adaptation

A Model Study of the Thermal Evolution of Astrophysical Ices

We address the question of the evolution of ices that have been exposed to radiation from stellar sources and cosmic rays. We studied in the laboratory the thermal evolution of a model ice sample: a mixture of water, hydrogen peroxide, dioxygen, and ozone produced by irradiating solid H2O2 with 50 keV H(+) at 17 K. The changes in composition and release of volatiles during warming to 200 K were monitored by infrared spectroscopy, mass spectrometry, and microbalance techniques. We find evidence for voids in the water component from the infrared bands due to dangling H bonds. The absorption from these bands increases during heating and can be observed at temperatures as high as approx. 155 K. More O2 is stored in the radiolyzed film than can be retained by codeposition of O2 and H2O. This O2 remains trapped until approx. 155 K, where it desorbs in an outburst as water ice crystallizes. Warming of the ice also drastically decreases the intrinsic absorbance of O2 by annealing defects in the ice. We also observe loss of O3 in two stages during heating, which correlates with desorption and possibly chemical reactions with radicals stored in the ice, triggered by the temperature increase.

Loeffler, M. J.

Evolution and status of the 30-cm engineering model ion thruster

In the past five years the 30-cm ion thruster has developed from infancy to maturity through the joint efforts of the NASA Lewis Research Center (LeRC) and the Hughes Research Laboratories (HRL). The evolution of the 30-cm thruster from the 200-series design to the present 900-series is described. This evolution has included both breadboard and engineering model type thrusters. The evolution description includes functional requirements, design, performance, endurance test results, and major features. The major part of the discussion centers on Hughes-built hardware although NASA LeRC contributions are reflected in the designs.

Masek, T. D.

Developmental Gene Regulation and Mechanisms of Evolution

The Marine Biological Laboratory and the National Aeronautics and Space Administration have established a cooperative agreement with the formation of a Center for Advanced Studies 'in the Space Life Sciences (CASSLS) at the MBL. This Center serves as an interface between NASA and the basic science community, addressing issues of mutual interest. The Center for Advanced Studies 'in the Space Life Sciences provides a forum for scientists to think and discuss, often for the first time, the role that gravity and aspects of spaceflight may play 'in fundamental cellular and physiologic processes. In addition the Center will sponsor discussions on evolutionary biology. These interactions will inform the community of research opportunities that are of interest to NASA. This workshop is one of a series of symposia, workshops and seminars that will be held at the MBL to advise NASA on a wide variety of topics in the life sciences, including cell biology, developmental biology, mg evolutionary biology, molecular biology, neurobiology, plant biology and systems biology.

Source record

Impacts of object-oriented technologies: Seven years of SEL studies

The premise that object-oriented technology (OOT) is the most significant technology ever examined by the Software Engineering Laboratory is examined. The evolution of the use of OOT in the Software Engineering Laboratory (SEL) 'experience factory' is described in terms of the SEL's original expectations, focusing on how successive generations of projects have used OOT. General conclusions are drawn on how the usage of the technology has evolved in this environment.

Stark, Michael

Technical note: Optimizing the in situ cosmogenic 36 Cl extraction and measurement workflow for geologic applications

Abstract. In situ cosmogenic 36Cl analysis by accelerator mass spectrometry (AMS) is routinely employed to date Quaternary surfaces and assess rates of landscape evolution. However, standard laboratory preparation procedures for 36Cl dating require the addition of large amounts of isotopically enriched chlorine spike solution; these solutions are expensive and increasingly difficult to acquire from commercial sources. In addition, the typical workflow for 36Cl dating involves measuring both 35Cl/37Cl and 36Cl/Cl concurrently on the high-energy (post-accelerator) end of the AMS system, but 35Cl/37Cl determinations using this technique can be complicated by isotope fractionation and system memory during measurement. The traditional workflow also does not provide 36Cl extraction laboratories with the data needed to calculate native Cl concentrations in advance of 36Cl/Cl measurements. In light of these concerns, we present an improved workflow for extracting and measuring chlorine in geologic materials. Our initial step is to characterize 35Cl/37Cl on sample aliquots of up to ∼1 g prepared in Ag(Cl, Br) matrices, which greatly reduces the amount of isotopically enriched spike solution required to measure native Cl content in each sample. To avoid potential issues with isotope fractionation through the accelerator, 35Cl/37Cl is measured on the low-energy, pre-accelerator end of the AMS line. Then, for 36Cl/Cl measurements, we extract Cl as AgCl or Ag(Cl, Br) in analytical batches with a consistent total Cl load across all samples; this step is intended to minimize source memory effects during 36Cl/Cl measurements and allows the preparation of AMS standards that are customized to match known Cl contents in the samples. To assess the efficacy of this extraction and measurement workflow, we compare chlorine isotope ratio measurements on seven geologic samples prepared using standard procedures and the updated workflow. Measurements of 35Cl/37Cl and 36Cl/Cl are consistent between the two workflows, and 35Cl/37Cl values measured using our methods have considerably higher precision than those measured following standard protocols. The chemical preparation and measurement workflow presented here (1) reduces the amount of isotopically enriched chlorine spike used per rock sample by up to 95 %; (2) identifies rocks with high native Cl concentrations, which may be lower priority for 36Cl surface exposure dating, at an early stage of analysis; and (3) allows laboratory users to maintain control over the total chlorine content within and across analytical batches. These methods can be incorporated into existing laboratory and AMS protocols for 36Cl analyses and will increase the accessibility of 36Cl dating for geologic applications.

58 GEOSCIENCES

Interstellar molecules and the origin of life.

Synopsis of the various views expressed at the conference held at NASA Ames Research Center in February 1971 on the relationship of interstellar molecules to the origin of life, intended to provide a basis for future discussion and work in this area. The topics covered include: a summary of molecules discovered, the interstellar environment, laboratory measurements, chemical evolution, and exobiology.

Buhl, D.

Web Design for Space Operations: An Overview of the Challenges and New Technologies Used in Developing and Operating Web-Based Applications in Real-Time Operational Support Onboard the International Space Station, in Astronaut Mission Planning and Mission Control Operations

The International Space Station (ISS) Operations Planning Team, Mission Control Centre and Mission Automation Support Network (MAS) have all evolved over the years to use commercial web-based technologies to create a configurable electronic infrastructure to manage the complex network of real-time planning, crew scheduling, resource and activity management as well as onboard document and procedure management required to co-ordinate ISS assembly, daily operations and mission support. While these Web technologies are classified as non-critical in nature, their use is part of an essential backbone of daily operations on the ISS and allows the crew to operate the ISS as a functioning science laboratory. The rapid evolution of the internet from 1998 (when ISS assembly began) to today, along with the nature of continuous manned operations in space, have presented a unique challenge in terms of software engineering and system development. In addition, the use of a wide array of competing internet technologies (including commercial technologies such as .NET and JAVA ) and the special requirements of having to support this network, both nationally among various control centres for International Partners (IPs), as well as onboard the station itself, have created special challenges for the MCC Web Tools Development Team, software engineers and flight controllers, who implement and maintain this system. This paper presents an overview of some of these operational challenges, and the evolving nature of the solutions and the future use of COTS based rich internet technologies in manned space flight operations. In particular this paper will focus on the use of Microsoft.s .NET API to develop Web-Based Operational tools, the use of XML based service oriented architectures (SOA) that needed to be customized to support Mission operations, the maintenance of a Microsoft IIS web server onboard the ISS, The OpsLan, functional-oriented Web Design with AJAX

Khan, Ahmed

Laboratory Calibration Studies in Support of ORGANICS on the International Space Station: Evolution of Organic Matter in Space

This paper describes the scientific overview and current status of ORGANICS an exposure experiment performed on the International Space Station (ISS) to study the evolution of organic matter in space (PI: P. Ehrenfreund), with supporting laboratory experiments performed at NASA Ames. ORGANICS investigates the chemical evolution of samples submitted to long-duration exposure to space environment in near-Earth orbit. This experiment will provide information on the nature, evolution, and survival of carbon species in the interstellar medium (ISM) and in solar system targets.

Ruiterkamp, R.

Tracing the Evolution of Hydrogen in the Martian Crust Through Laboratory Studies of Apatite

Introduction: Northwest Africa (NWA) 7034 and its pairings represent a regolith breccia of basaltic bulk composition, the finegrained matrix of which bears a strong resemblance to the major and trace element composition estimated for the ancient southern highlands crust on Mars. Therefore, NWA 7034 may represent a key sample for constraining the composition of the Martian crust, particularly the ancient highlands. Here we seek to constrain the hydrogen isotopic composition of the Martian crust using apatite [Ca5 (PO4)3(Cl,F,OH)]. Apatites across all lithologic domains in NWA 7034 have been affected by a Pb-loss event at ~1.5 Ga before present and so they are unlikely to have retained magmatic volatile composition and are more likely to have equilibrated with fluids within the Martian crust that may or may not have exchanged with the Martian atmosphere.

Barnes, Jessica

The Chemical Evolution of Cosmic Dust: From Gas-Phase Precursors to Solid Grains - Laboratory Studies with the COSmIC Facility

Cosmic dust in in its gas phase and solid phase components plays an essential role in the chemical and physical evolution of the universe. Our understanding of cosmic dust properties relies on a combination of astronomical observations, astrophysical modeling and laboratory studies of realistic analogs of cosmic dust being gas-phase molecules and/or solid grains. The COSmIC facility was developed at NASA Ames to study the evolution of cosmic carbon from circumstellar outflows to interstellar clouds to planetary atmospheres in the laboratory [1]. COSmIC stands for “Cosmic Simulation Chamber” and is dedicated to the study of neutral and ionized molecules and grain particles under the low temperature and density conditions that are required to simulate space environments. It integrates a variety of instruments that allow generating, processing, and monitoring simulated space conditions in the laboratory. It is composed of a Pulsed Discharge Nozzle (PDN) expansion that generates a plasma in a free supersonic jet expansion, coupled to high-sensitivity, complementary in situ diagnostic tools used for the detection and characterization of the species present in the expansion: Cavity Ring Down Spectroscopy (CRDS) and fluorescence spectroscopy systems for photonic detection and an orthogonal Reflectron Time-Of-Flight Mass Spectrometer (oReTOF-MS) for mass detection. Recent advances achieved using COSmIC’s laboratory data in synergy with observational data will be presented. These results include the formation of dust grains and aerosols from gas-phase molecular precursors in environments as varied as circumstellar outflows of late AGB stars [2, 3] and planetary atmospheres [4] and the evolution of our understanding of the diffuse interstellar bands (DIBs) with applications to the ESO Diffuse Interstellar Bands Large Exploration Survey (EDIBLES [5, 6]). Plans for future laboratory developments and techniques to study the evolution of cosmic carbon molecules and grains (including NIR-MIR CRDS, laser induced fluorescence (LIF) and incandescence (LII)) will also be addressed as well as their astronomical applications.

Planetary

The Chemical Evolution of Cosmic Dust: From Gas-Phase Precursors to Solid Grains - Laboratory Studies with the COSmIC Facility

Cosmic dust in in its gas phase and solid phase components plays an essential role in the chemical and physical evolution of the universe. Our understanding of cosmic dust properties relies on a combination of astronomical observations, astrophysical modeling and laboratory studies of realistic analogs of cosmic dust being gas-phase molecules and/or solid grains. The COSmIC facility was developed at NASA Ames to study the evolution of cosmic carbon from circumstellar outflows to interstellar clouds to planetary atmospheres in the laboratory. COSmIC stands for “Cosmic Simulation Chamber” and is dedicated to the study of neutral and ionized molecules and grain particles under the low temperature and density conditions that are required to simulate space environments. It integrates a variety of instruments that allow generating, processing, and monitoring simulated space conditions in the laboratory. It is composed of a Pulsed Discharge Nozzle (PDN) expansion that generates a plasma in a free supersonic jet expansion, coupled to high-sensitivity, complementary in situ diagnostic tools used for the detection and characterization of the species present in the expansion: Cavity Ring Down Spectroscopy (CRDS) and fluorescence spectroscopy systems for photonic detection and an orthogonal Reflectron Time-Of-Flight Mass Spectrometer (oReTOF-MS) for mass detection. Recent advances achieved using COSmIC’s laboratory data in synergy with observational data will be presented. These results include the formation of dust grains and aerosols from gas-phase molecular precursors in environments as varied as circumstellar outflows of late AGB stars and planetary atmospheres [4] and the evolution of our understanding of the diffuse interstellar bands (DIBs) with applications to the ESO Diffuse Interstellar Bands Large Exploration Survey (EDIBLES). Plans for future laboratory developments and techniques to study the evolution of cosmic carbon molecules and grains (including NIR-MIR CRDS, laser induced fluorescence (LIF) and incandescence (LII)) will also be addressed as well as their astronomical applications.

Farid Salama

Relay Support for the Mars Science Laboratory and the Coming Decade of Mars Relay Network Evolution

In the past decade, an evolving network of Mars relay orbiters has provided telecommunication relay services to the Mars Exploration Rovers, Spirit and Opportunity, and to the Mars Phoenix Lander, enabling high-bandwidth, energy-efficient data transfer and greatly increasing the volume of science data that can be returned from the Martian surface, compared to conventional direct-to-Earth links. The current relay network, consisting of NASA's Odyssey and Mars Reconnaissance Orbiter and augmented by ESA's Mars Express Orbiter, stands ready to support the Mars Science Laboratory, scheduled to arrive at Mars on Aug 6, 2012, with new capabilities enabled by the Electra and Electra-Lite transceivers carried by MRO and MSL, respectively. The MAVEN orbiter, planned for launch in 2013, and the ExoMars/Trace Gas Orbiter, planned for launch in 2016, will replenish the on-orbit relay network as the current orbiter approach their end of life. Currently planned support scenarios for this future relay network include an ESA EDL Demonstrator Module deployed by the 2016 ExoMars/TGO orbiter, and the 2018 NASA/ESA Joint Rover, representing the first step in a multimission Mars Sample Return campaign.

MAVEN

Probing molecular wavepacket evolution using multidimensional coherent spectroscopy (Final Report)

The goal of this project was to develop and demonstrate multidimensional coherent spectroscopy (MDCS) techniques to fully map wave-packet dynamics in simple molecules. MDCS correlates excitation and emis sion frequencies, enabling continuous tracking of wave-packet evolution even when multiple wave-packets evolve simultaneously. The initial experiments were to be performed using the MDCS apparatus in the PI’s laboratory to record the evolution of the wave-packet’s energy in molecular iodine. A longer-term goal was to develop a version compatible with using an X-ray Free Electron Laser (XFEL) as a probe, which would allow the evolution of the internuclear distance of the wave-packet—not just its energy—to be determined.

74 ATOMIC AND MOLECULAR PHYSICS

Relay Support for the Mars Science Laboratory and the Coming Decade of Mars Relay Network Evolution

Mars Relay Network is prepared to support MSL: a) ODY/MRO/MEX will all provide critical event comm support during EDL. b) New Electra/Electra-Lite capabilities on the MSL-MRO link will support >250 Mb/sol MSL data return. 2013 MAVEN orbiter will replenish on-orbit relay infrastructure as prior orbiters approach end-of-life. While NASA has withdrawn from the 2016 EMTGO and 2018 Joint Rover missions, analysis of the potential link shows a path to Gbit/sol relay capability 2012.

critical event telemetry

Evolution of catalytic function

An RNA-based evolution system was constructed in the laboratory and used to develop RNA enzymes with novel catalytic function. By controlling the nature of the catalytic task that the molecules must perform in order to survive, it is possible to direct the evolving population toward the expression of some desired catalytic behavior. More recently, this system has been coupled to an in vitro translation procedure, raising the possibility of evolving protein enzymes in the laboratory to produce novel proteins with desired catalytic properties. The aim of this line of research is to reduce darwinian evolution, the fundamental process of biology, to a laboratory procedure that can be made to operate in the service of organic synthesis.

NASA Program Exobiology

The European Spacelab structural design evolution

Spacelab is a manned, reusable laboratory which is being developed for the European Space Agency (ESA). In its working mode it will fly in low earth orbit in the cargo bay of the Shuttle Transportation System (STS) Orbiter. A description is presented of the structural development of the various features of Spacelab. System requirements are considered along with structural requirements, quasi-static loads, acoustic loads, pressure loads, crash loads, ground loads, and the fatigue profile. Aspects of thermal environment generation are discussed, and questions regarding the design evolution of the pallet structure are examined. Details of pallet structure testing are reported, taking into account static strength tests, acoustic tests, the modal survey test, crash tests, and fatigue/fracture mechanics testing.

Thirkettle, A. J.