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At least 73 records · Page 4

Measurements of $\pi ^\pm $, $K^\pm $, p and $\bar{p}$ spectra in $^7$Be+$^9$Be collisions at beam momenta from 19A to 150A ${\mathrm{Ge} \mathrm{V}}\!/\!c$ with the NA61/SHINE spectrometer at the CERN SPS

The NA61/SHINE experiment at the CERN Super Proton Synchrotron (SPS) studies the onset of deconfinement in hadron matter by a scan of particle production in collisions of nuclei with various sizes at a set of energies covering the SPS energy range. This paper presents results on inclusive double-differential spectra, transverse momentum and rapidity distributions and mean multiplicities of $\pi^, \pm K^\pm$ p and $\bar{p}$ produced in the 20% most central Be+$^9$ Be collisions at beam momenta of 19A, 30A, 40A, 75A and 150A ${\mathrm{Ge} \mathrm{V}}\!/\!c$. The energy dependence of the $K^\pm/ \pi^\pm$ ratios as well as of inverse slope parameters of the $K^\pm$ transverse mass distributions are close to those found in inelastic $p+p$ reactions. The new results are compared to the world data on $p+p and Pb+Pb collisions as well as to predictions of the Epos , U r qmd , Ampt , Phsd and Smash models.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Measurements of $\pi ^\pm $, $K^\pm $, p and $\bar{p}$ spectra in $^{40}\hbox {Ar+}^{45}\hbox {Sc}$ collisions at 13A to 150A $\text{ Ge }\hspace{-1.00006pt}\text{ V }\!/\!c$

The NA61/SHINE experiment at the CERN Super Proton Synchrotron studies the onset of deconfinement in strongly interacting matter through a beam energy scan of particle production in collisions of nuclei of varied sizes. This paper presents results on inclusive double-differential spectra, transverse momentum and rapidity distributions and mean multiplicities of $\pi ^\pm $, $K^\pm $, p and $\bar{p}$ produced in $^{40}\hbox {Ar+}^{45}\hbox {Sc}$ collisions at beam momenta of 13A, 19A, 30A, 40A, 75A and 150A $\text{ Ge }\hspace{-1.00006pt}\text{ V }\!/\!c$. The analysis uses the 10% most central collisions, where the observed forward energy defines centrality. The energy dependence of the $K^\pm $/$\pi ^\pm $ ratios as well as of inverse slope parameters of the $K^\pm $ transverse mass distributions are placed in between those found in inelastic $p+p$ and central Pb + Pb collisions. The results obtained here establish a system-size dependence of hadron production properties that so far cannot be explained either within statistical or dynamical models.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Size-resolved Eddy-Covariance Particle Flux Measurement during the TRACER Campaign (Final Report)

The main goal of the TRacking Aerosol Convection interactions ExpeRiment (TRACER) campaign was to study aerosol–cloud interactions during deep convection over the Houston area. This project deployed a suite of instrumentation with the aim to (1) quantify turbulent vertical particle fluxes during at DOE-ARM sites, including TRACER, (2) assess hygroscopic growth factors and hygroscopicity parameters of the material driving modal aerosol growth during new particle formation and growth events, (3) derive turbulent aerosol mass fluxes using co-located Doppler LIDAR measurements, and (4) create quality-controlled PI data products to support future research utilizing data collected during the TRACER campaign. This report summarized the main findings from the deployments at two DOE-ARM sites. Briefly, we found that new particle formation may occur aloft, in a residual layer, near the top of the boundary layer. Small grown particles appear later due to downward mixing with daytime turbulence. The species that are responsible for aerosol modal growth had hygroscopicity parameters varying between 0.05 and 0.34. These values systematically depended on the wind sector, suggesting that the chemical composition of the precursors differed. This work demonstrated that lidar retrievals of the elastic backscatter and Doppler velocity can be used to obtain surface number emissions of particles with a diameter greater than 0.53 µm. During TRACER, emission particle number fluxes peaked near ∼ 100 cm−2 s−1. Multiple quality-controlled PI data products that will support future TRACER related science were generated and made publically available.

54 ENVIRONMENTAL SCIENCES↗

Direct time-resolved detection and quantification of key reactive intermediates in diethyl ether oxidation at T = 450–600 K

High-pressure multiplexed photoionization mass spectrometry (MPIMS) with tunable vacuum ultraviolet (VUV) ionization radiation from the Lawrence Berkeley Labs Advanced Light Source is used to investigate the oxidation of diethyl ether (DEE). Kinetics and photoionization (PI) spectra are simultaneously measured for the species formed. Several stable products from DEE oxidation are identified and quantified using reference PI cross-sections. In addition, we directly detect and quantify three key chemical intermediates: peroxy (ROO˙), hydroperoxyalkyl peroxy (˙OOQOOH), and ketohydroperoxide (HOOP[double bond, length as m-dash]O, KHP). These intermediates undergo dissociative ionization (DI) into smaller fragments, making their identification by mass spectrometry challenging. Furthermore, with the aid of quantum chemical calculations, we identify the DI channels of these key chemical species and quantify their time-resolved concentrations from the overall carbon atom balance at T = 450 K and P = 7500 torr. This allows the determination of the absolute PI cross-sections of ROO˙, ˙OOQOOH, and KHP into each DI channel directly from experiment. The PI cross-sections in turn enable the quantification of ROO˙, ˙OOQOOH, and KHP from DEE oxidation over a range of experimental conditions that reveal the effects of pressure, O 2 concentration, and temperature on the competition among radical decomposition and second O 2 addition pathways.

42 ENGINEERING↗

Gas–Phase Preparation of the 14π Hückel Polycyclic Aromatic Anthracene and Phenanthrene Isomers (C 14 H 10 ) via the Propargyl Addition–BenzAnnulation (PABA) Mechanism

Polycyclic aromatic hydrocarbons (PAHs) imply the missing link between resonantly stabilized free radicals and carbonaceous nanoparticles, commonly referred to as soot particles in combustion systems and interstellar grains in deep space. Whereas gas phase formation pathways to the simplest PAH – naphthalene (C 10 H 8 ) – are beginning to emerge, reaction pathways leading to the synthesis of the 14π Hückel aromatic PAHs anthracene and phenanthrene (C 14 H 10 ) are still incomplete. Here, by utilizing a chemical microreactor in conjunction with vacuum ultraviolet (VUV) photoionization (PI) of the products followed by detection of the ions in a reflectron time-of-flight mass spectrometer (ReTOF-MS), the reaction between the 1'- and 2'-methylnaphthyl radicals (C 11 H 9 •) with the propargyl radical (C 3 H 3 •) accesses anthracene (C 14 H 10 ) and phenanthrene (C 14 H 10 ) via the Propargyl Addition–BenzAnnulation (PABA) mechanism in conjunction with a hydrogen assisted isomerization. Furthermore, the preferential formation of the thermodynamically less stable anthracene isomer compared to phenanthrene suggests a kinetic, rather than a thermodynamics control of the reaction.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Speciated non-methane hydrocarbon measurements at the Oliktok Point, AK AMF3 from February-March 2020

This dataset contains speciated non-methane hydrocarbon gas-phase mole ratio measurements at the Oliktok Point, AK AMF3 as measured by a Tofwerk Vocus-2R time-of-flight NO+ chemical ionization mass spectrometer (TOF-NO+-CIMS) coupled with an Aerodyne gas chromatograph (GC). Users of the data are asked to contact PI Pratt prior to use for consultation, best data use, discussion of co-authorship, and to ensure that the most up-to-date information is provided.

benzene↗

New insights into the doubly charmed exotic mesons

Abstract Using effective Lagrangians constrained by the heavy quark spin symmetry and chiral symmetry, for the light quarks, we analyze the $$D^0 D^0\pi ^+,$$ D 0 D 0 π + , $$\bar{D}^0D^0\pi ^0$$ D ¯ 0 D 0 π 0 and $$D^0\bar{D}^{*0}$$ D 0 D ¯ ∗ 0 invariant mass spectra. Performing a simultaneous analysis of the doubly charmed and charm-anti-charm states gives further insights into the nature of the $$T^+_{cc}$$ T cc + and $$\chi ^0_{c1}(3872),$$ χ c 1 0 ( 3872 ) , exotic hadrons. It is confirmed that both states should lie below their respective $$DD^*$$ D D ∗ / $$D\bar{D}^*$$ D D ¯ ∗ thresholds. Also, the contributions of the triangle and box diagrams are negligible.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

$\bar{\nu}_\mu$ charged-current $\pi^0$ data release

Data release for the NOvA muon antineutrino charged-current (CC) pi^0 cross section presented in arXiv:2511.05807. The signal for this analysis is defined as muon antineutrino CC interactions in the fiducial volume of the NOvA near detector (a 2.7 m × 2.7 m × 9.0 m region) that produce at least one pi^0 in the final state emerging from the nucleus, within the phase space of muon momentum [0.5, 2.5) GeV/c and muon angle [0, 60) degree, as described in arXiv:2511.05807. The released zip file contains two files: NOvA_NumubarCCPi0_DataRelease.root README.txt The ROOT file includes the cross-section results as well as the statistical and systematic covariance matrices for each variable used in this analysis. The README provides a detailed description of the contents of the data release. Official Flux: The flux used in this analysis is available from the NOvA Public Docs: https://publicdocs.fnal.gov/cgi-bin/ShowDocument?docid=8. File structure --- The ROOT file contains the following TDirectories: pi0p - pi^0 momentum distributions pi0dir - pi^0 angular distributions muonp - muon momentum distributions muondir - muon angular distributions Q2 - reconstructed Q^2 distributions Wmass - reconstructed W_mass distributions Each directory contains three histograms: xsec (TH1D): Cross section result cov_stat (TH2D): Statistical covariance matrix cov_syst (TH2D): Systematic covariance matrix Usage notes: - xsec gives the measured differential cross section w.r.t. the corresponding variable. - cov_stat and cov_syst provide the full covariance matrices. - The bin definitions and kinematic phase spaces follow those used in arXiv:2511.05807. Citation --- If you use these data, please cite: NOvA Collaboration, arXiv:2511.05807.

Wu, Wanwei [Pittsburgh U.] (ORCID:0000000326327215↗

Production of light-flavor hadrons in pp collisions at $\sqrt{s}~=~7\text { and }\sqrt{s} = 13 \, \text { TeV} $

The production of \(\pi ^{\pm }\) , \(\mathrm{K}^{\pm }\) , \(\mathrm{K}^{0}_{S}\) , \(\mathrm{K}^{*}(892)^{0}\) , \(\mathrm{p}\) , \(\phi (1020)\) , \(\Lambda \) , \(\Xi ^{-}\) , \(\Omega ^{-}\) , and their antiparticles was measured in inelastic proton–proton (pp) collisions at a center-of-mass energy of \(\sqrt{s}\) = 13 TeV at midrapidity ( \(|y|<0.5\) ) as a function of transverse momentum ( \(p_{\mathrm{T}}\) ) using the ALICE detector at the CERN LHC. Furthermore, the single-particle \(p_{\mathrm{T}}\) distributions of \(\mathrm{K}^{0}_{S}\) , \(\Lambda \) , and \(\overline{\Lambda }\) in inelastic pp collisions at \(\sqrt{s} = 7\) TeV are reported here for the first time. The \(p_{\mathrm{T}}\) distributions are studied at midrapidity within the transverse momentum range \(0\le p_{\mathrm{T}}\le 20\) GeV/ c , depending on the particle species. The \(p_{\mathrm{T}}\) spectra, integrated yields, and particle yield ratios are discussed as a function of collision energy and compared with measurements at lower \(\sqrt{s}\) and with results from various general-purpose QCD-inspired Monte Carlo models. A hardening of the spectra at high \(p_{\mathrm{T}}\) with increasing collision energy is observed, which is similar for all particle species under study. The transverse mass and \(x_{\mathrm{T}}\equiv 2p_{\mathrm{T}}/\sqrt{s}\) scaling properties of hadron production are also studied. As the collision energy increases from \(\sqrt{s}\) = 7–13 TeV, the yields of non- and single-strange hadrons normalized to the pion yields remain approximately constant as a function of \(\sqrt{s}\) , while ratios for multi-strange hadrons indicate enhancements. The \(p_\mathrm{{T}}\) -differential cross sections of \(\pi ^{\pm }\) , \(\mathrm {K}^{\pm }\) and \(\mathrm {p}\) ( \(\overline{\mathrm{p}}\) ) are compared with next-to-leading order perturbative QCD calculations, which are found to overestimate the cross sections for \(\pi ^{\pm }\) and \(\mathrm{p}\) ( \(\overline{\mathrm{p}}\) ) at high \(p_\mathrm{{T}}\) .

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗

Time-resolved quantification of key species and mechanistic insights in low-temperature tetrahydrofuran oxidation

Here, we investigate the kinetics and report the time-resolved concentrations of key chemical species in the oxidation of tetrahydrofuran (THF) at 7500 torr and 450–675 K. Experiments are carried out using high-pressure multiplexed photoionization mass spectrometry (MPIMS) combined with tunable vacuum ultraviolet radiation from the Berkely Lab Advanced Light Source. Intermediates and products are quantified using reference photoionization (PI) cross sections, when available, and constrained by a global carbon balance tracking approach at all experimental temperatures simultaneously for the species without reference cross sections. From carbon balancing, we determine time-resolved concentrations for the ROO˙ and ˙OOQOOH radical intermediates, butanedial, and the combined concentration of ketohydroperoxide (KHP) and unsaturated hydroperoxide (UHP) products stemming from the ˙QOOH + O 2 reaction. Furthermore, we quantify a product that we tentatively assign as fumaraldehyde, which arises from UHP decomposition via H 2 O or ˙OH + H loss. The experimentally derived species concentrations are compared with model predictions using the most recent literature THF oxidation mechanism of Fenard et al., (Combust. Flame, 2018, 191, 252–269). Our results indicate that the literature mechanism significantly overestimates THF consumption and the UHP + KHP concentration at our conditions. The model predictions are sensitive to the rate coefficient for the ROO˙ isomerization to ˙QOOH, which is the gateway for radical chain propagating and branching pathways. Comparisons with our recent results for cyclopentane (Demireva et al., Combust. Flame, 2023, 257, 112506) provide insights into the effect of the ether group on reactivity and highlight the need to determine accurate rate coefficients of ROO˙ isomerization and subsequent reactions.

Demireva, Maria↗

Extraction of Beam-Spin Asymmetries from the Hard Exclusive π + Channel off Protons in a Wide Range of Kinematics

We have measured beam-spin asymmetries to extract the $\sin\phi$ moment $A_{LU}^{\sin\phi}$ from the hard exclusive $\vec{e} p \to e^\prime n \pi^+$ reaction above the resonance region, for the first time with nearly full coverage from forward to backward angles in the center-of-mass. The $A_{LU}^{\sin\phi}$ moment has been measured up to 6.6 GeV$^{2}$ in $-t$, covering the kinematic regimes of Generalized Parton Distributions (GPD) and baryon-to-meson Transition Distribution Amplitudes (TDA) at the same time. The experimental results in very forward kinematics demonstrate the sensitivity to chiral-odd and chiral-even GPDs. In very backward kinematics where the TDA framework is applicable, we found $A_{LU}^{\sin\phi}$ to be negative, while a sign change was observed near 90$^\circ$ in the center-of-mass. The unique results presented in this paper will provide critical constraints to establish reaction mechanisms that can help to further develop the GPD and TDA frameworks.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Testing Lepton Flavor Universality and CKM Unitarity with Rare Pion Decays in the PIONEER experiment

The physics motivation and the conceptual design of the PIONEER experiment, a next-generation rare pion decay experiment testing lepton flavor universality and CKM unitarity, are described. Phase I of the PIONEER experiment, which was proposed and approved at Paul Scherrer Institut, aims at measuring the charged-pion branching ratio to electrons vs. muons, $R_{e/\mu}$, 15 times more precisely than the current experimental result, reaching the precision of the Standard Model (SM) prediction at 1 part in $10^4$. Considering several inconsistencies between the SM predictions and data pointing towards the potential violation of lepton flavor universality, the PIONEER experiment will probe non-SM explanations of these anomalies through sensitivity to quantum effects of new particles up to the PeV mass scale. The later phases of the PIONEER experiment aim at improving the experimental precision of the branching ratio of pion beta decay (BRPB), $\pi^+\to \pi^0 e^+ \nu (\gamma)$, currently at $1.036(6)\times10^{-8}$, by a factor of three (Phase II) and an order of magnitude (Phase III). Such precise measurements of BRPB will allow for tests of CKM unitarity in light of the Cabibbo Angle Anomaly and the theoretically cleanest extraction of $|V_{ud}|$ at the 0.02% level, comparable to the deduction from superallowed beta decays.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Study of Υ(10753) decays to π+π−Υ(nS) final states at Belle II

Abstract We present an analysis of the processe + e − →π + π − Υ(nS) (wheren= 1, 2, or 3) reconstructed in 19.6 fb −1 of Belle II data during a special run of the SuperKEKB collider at four energy points near the peak of the Υ(10753) resonance. By analyzing the mass distribution of theπ + π − Υ(nS) system and the Born cross sections of thee + e − →π + π − Υ(nS) process, we report the first observation of Υ(10753) decays to theπ + π − Υ(1S) andπ + π − Υ(2S) final states, and find no evidence for decays toπ + π − Υ(3S). Possible intermediate states in theπ + π − Υ(1S,2S) transitions are also investigated, and no evidence for decays proceeding via the$$ {\pi}^{\mp }{Z}_b^{\pm } $$ π ∓ Z b ± orf 0 (980)Υ(nS) intermediate states is found. We measure Born cross sections for thee + e − →π + π − Υ(nS) process that, combined with results from Belle, obtain the mass and width of Υ(10753) to be (10756.6 ± 2.7 ± 0.9) MeV/c 2 and (29.0 ± 8.8 ± 1.2) MeV, respectively. The relative ratios of the Born cross sections at the Υ(10753) resonance peak are also reported for the first time.

Physics↗

Study of central exclusive $\pi^+\pi^-$ production in proton-proton collisions at $\sqrt{s} =$ 5.02 and 13 TeV

Central exclusive and semiexclusive production of pairs is measured with the CMS detector in proton-proton collisions at the LHC at center-of-mass energies of 5.02 and 13TeV. The theoretical description of these nonperturbative processes, which have not yet been measured in detail at the LHC, poses a significant challenge to models. The two pions are measured and identified in the CMS silicon tracker based on specific energy loss, whereas the absence of other particles is ensured by calorimeter information. The total and differential cross sections of exclusive and semiexclusive central production are measured as functions of invariant mass, transverse momentum, and rapidity of the system in the fiducial region defined as transverse momentum and pseudorapidity . The production cross sections for the four resonant channels [inline-graphic not available: see fulltext], , [inline-graphic not available: see fulltext], and [inline-graphic not available: see fulltext]are extracted using a simple model. These results represent the first measurement of this process at the LHC collision energies of 5.02 and 13TeV.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Search for Lepton Flavor Violation in Two Body Muon and Pion Decay at Rest

The ability of the Mu2e experiment to probe, or discover beyond the Standard Model physics in direct Charged Lepton Flavor Violation $\mu^+$ and $\pi^+$ decay modes is estimated. These direct modes are searched for simultaneously with proposed Mu2e detector validation runs, and are complementary to the Mu2e main search goal, an indirect search for $\mu^- \to e^-$ conversion at the sensitivity level of $\sim 10^{-17}$. The $\mu^+$ validation run will operate at 50% nominal magnetic field and reduced proton beam intensity to less than 1/100th nominal, in order to observe the e+ spectrum from $\mu^+$ decay, at and below the Michel edge Ee . 53 MeV. The $\pi^+$ validation run, based on measuring the mono-energetic e+ emission in the decay $\pi^+ \to e+\nu$, at 76% of nominal magnetic field and reduced beam intensity less than 1/5th nominal. Both of these runs can be used to fix the momentum scale for the Mu2e conversion search. In addition the muon validation dataset can be used to correct for systematic errors in the detector response by mapping the well known to O(\u03B13) corrected theoretical Michel spectrum, to the observed spectrum. One direct search is for two-body Charged Lepton Flavor Violation $\mu^+ \to e^+X$ decay, where $X$ is a light new physics particle. This allows Mu2e to explore well motivated models including axion like particles with flavor violating couplings and massive $Z^0$ bosons with more sensitivity then present astrophysical and laboratory constraints. In two weeks of data-taking, Mu2e can achieve direct mode 90% confidence level branching ratio limits of $10^{-7}$ over the mass range $20 \le m_X \le 50$ MeV, improving the current experimental limit at $10^{-5}$ by two orders of magnitude. In the mass range $m_X \le 20$ MeV, assuming systematic error corrections can be made by correcting the Monte Carlo mapping, the achievable search sensitivity is found to be $2.3\times 10^{-7}$ for $m_X =0$, an order of magnitude improvement over the current best limit at $2.6\times 10^{-6}$, when assuming $V+A$ or isotropic coupling. The $\pi^+$ validation run, allows searching for $\pi^+ \to e^+N$ decay, where $N$ is a heavy neutral lepton such as a heavy sterile neutrino, in the mass region $20 \le m_N \le 65$ MeV. A branching ratio limit at 90% confidence level of $3\times 10^{-8}$ can be achieved in two weeks, an improvement of the current search sensitivity limit by an order of magnitude.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Three-pion effects in $K^0-\bar{K}^0$ mixing

The rate of mixing between a neutral kaon and an anti-kaon ($K^0$-$\bar{K}^0$) is given, in part, by a long-range matrix element, defined with two insertions of the weak Hamiltonian separated by physical, Minkowski time evolution. For physical quark masses, the kaon mass lies above the two- and three-pion thresholds and, as a result, this long-range matrix element receives contributions from intermediate on-shell $2\pi$ and $3\pi$ states. These contributions cannot easily be captured in a finite Euclidean spacetime, meaning that such matrix elements are not directly accessible via lattice QCD. In this talk, we present a strategy for combining quantities that can be extracted in numerical lattice QCD calculations in order to reproduce the physical, infinite-volume long-range amplitude for $K^0-\bar{K}^0$. The key novelty relative to published work is that we fully include the effects of three-particle states that were previously neglected. The strategy is built on existing formalism for long-range matrix elements with two-particle intermediate states, together with the relativistic-field-theory finite-volume formalism for extracting three-hadron weak decays.

Jackura, Andrew↗

Single $\pi ^0$ production off neutrons bound in deuteron with linearly polarized photons

The quasifree γ → d → π 0 n(p) photon beam asymmetry, Σ, has been measured at photon energies, E γ , from 390 to 610 MeV, corresponding to center of mass energy from 1.271 to 1.424 GeV, for the first time. The data were collected in the A2 hall of the MAMI electron beam facility with the Crystal Ball and TAPS calorimeters covering pion center-of-mass angles from 49° to 148°. In this kinematic region, polarization observables are sensitive to contributions from the Δ(1232) and N(1440) resonances. The extracted values of Σ have been compared to predictions based on partial-wave analyses (PWAs) of the existing pion photoproduction database. Our comparison includes the SAID, MAID and Bonn–Gatchina analyses; while a revised SAID fit, including the new Σ measurements, has also been performed. In addition, isospin symmetry is examined as a way to predict π 0 n photoproduction observables, based on fits to published data in the channels π 0 p, π + n and π – p.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Structural analysis of the PTEN:P-Rex2 signaling complex reveals how cancer-associated mutations coordinate to hyperactivate Rac1

The dual-specificity phosphatase PTEN functions as a tumor suppressor by hydrolyzing PI(3,4,5)P 3 to PI(4,5)P 2 to inhibit PI3K-AKT signaling and cellular proliferation. P-Rex2 is a guanine nucleotide exchange factor for Rho GTPases and can be activated by Gβγ subunits downstream of G protein–coupled receptor signaling and by PI(3,4,5)P 3 downstream of receptor tyrosine kinases. The PTEN:P-Rex2 complex is a commonly mutated signaling node in metastatic cancer. Assembly of the PTEN:P-Rex2 complex inhibits the activity of both proteins, and its dysregulation can drive PI3K-AKT signaling and cellular proliferation. Here, using cross-linking mass spectrometry and functional studies, we gained mechanistic insights into PTEN:P-Rex2 complex assembly and coinhibition. We found that PTEN was anchored to P-Rex2 by interactions between the PDZ-interacting motif in the PTEN C-terminal tail and the second PDZ domain of P-Rex2. This interaction bridged PTEN across the P-Rex2 surface, preventing PI(3,4,5)P 3 hydrolysis. Conversely, PTEN both allosterically promoted an autoinhibited conformation of P-Rex2 and blocked its binding to Gβγ. In addition, we observed that the PTEN-deactivating mutations and P-Rex2 truncations combined to drive Rac1 activation to a greater extent than did either single variant alone. These insights enabled us to propose a class of gain-of-function, cancer-associated mutations within the PTEN:P-Rex2 interface that uncouple PTEN from the inhibition of Rac1 signaling.

D’Andrea, Laura↗