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At least 73 records · Page 4

Influences of prostanoids and nitric oxide on post-suspension hypotension in female Sprague-Dawley rats

Impairment in cardiovascular functions sometimes manifested in astronauts during standing postflight, may be related to the diminished autonomic function and/or excessive production of endothelium-dependent relaxing factors. In the present study, using the 30 degrees head-down tilt (HDT) model, we compared the cardiovascular and biochemical effects of 7 days of suspension and a subsequent 6-h post-suspension period between suspended and non-suspended conscious female Sprague-Dawley rats. Mean arterial pressure (MAP) and heart rate were measured prior to suspension (basal), daily thereafter, and every 2h post-suspension. Following 7 days of suspension, MAP was not different from their basal values, however, upon release from suspension, MAP was significantly reduced compared to the non-suspended rats. Nitric oxide levels were elevated while thromboxane A(2) levels declined significantly in both plasma and tissue samples following post-suspension. The levels of prostacyclin following post-suspension remained unaltered in plasma and aortic rings but was significantly elevated in carotid arterial rings. Therefore, the post-suspension reduction in mean arterial pressure is due mostly to overproduction of nitric oxide and to a lesser extent prostacyclin.

Non-NASA Center

Determining the Optimum Post Spacing of LIDAR-Derived Elevation Data in Varying Terrain for Flood Hazard Mapping Purposes in North Carolina and Texas

The major flood events in the United States in the past few years have made it apparent that many floodplain maps being used by State governments are outdated and inaccurate. In response, many Stated have begun to update their Federal Emergency Management Agency (FEMA) Digital Flood Insurance Rate Maps. Accurate topographic data is one of the most critical inputs for floodplain analysis and delineation. Light detection and ranging (LIDAR) altimetry is one of the primary remote sensing technologies that can be used to obtain high-resolution and high-accuracy digital elevation data suitable for hydrologic and hydraulic (H&H) modeling, in part because of its ability to "penetrate" various cover types and to record geospatial data from the Earth's surface. However, the posting density or spacing at which LIDAR collects the data will affect the resulting accuracies of the derived bare Earth surface, depending on terrain type and land cover type. For example, flat areas are thought to require higher or denser postings than hilly areas to capture subtle changes in the topography that could have a significant effect on flooding extent. Likewise, if an area has dense understory and overstory, it may be difficult to receive LIDAR returns from the Earth's surface, which would affect the accuracy of that bare Earth surface and thus would affect flood model results. For these reasons, NASA and FEMA have partnered with the State of North Carolina and with the U.S./Mexico Foundation in Texas to assess the effect of LIDAR point density on the characterization of topographic variation and on H&H modeling results for improved floodplain mapping. Research for this project is being conducted in two areas of North Carolina and in the City of Brownsville, Texas, each with a different type of terrain and varying land cover/land use. Because of various project constraints, LIDAR data were acquired once at a high posting density and then decimated to coarser postings or densities. Quality assurance/quality control analyses were performed on each dataset. Cross sections extracted form the high density and then the decimated datasets were individually input into an H&H model to determine the model's sensitivity to topographic variation and the effect of that variation on the resulting water profiles. Additional analysis was performed on the Brownsville, Texas, LIDAR data to determine the percentage of returns that "penetrated" various types of canopy or vegetative cover. It is hoped that the results of these studies will benefit state and local communities as they consider the post spacing at which to acquire LIDAR data (which affects cost) and will benefit FEMA as the Agency assesses the use of different technologies for updating National Flood Insurance Program and related products.

Berglund, Judith

Rocky Mountain Disasters - Using NASA Earth Observations to Monitor Post-Fire Vegetation Recovery in the Colorado Front Range

Forest composition and structure in the Colorado Front Range has been altered by changing wildfire regimes. In particular, increased moderate- and high-severity fire significantly reduces forest cover following fire and often results in reduced seedling regeneration. Reduced tree canopy regrowth has chronic effects on upland ecological function and downstream water quality. This project partnered with the US Forest Service to estimate long-term vegetation recovery following four Colorado Front Range fires between 1996 and 2002—the Bobcat, Buffalo Creek, Hayman, and High Meadows fires—using Landsat 5 Thematic Mapper (TM),Landsat 7 Enhanced Thematic Mapper (ETM+), and Landsat 8 Operational Land Imager (OLI). The random forest algorithm was applied to produce maps of percent forest canopy cover for coniferous trees, deciduous trees, and all trees using time-series variables for pre- and post-fire as inputs. Similarly, maps of post-fire seedling regeneration were produced using random forest for coniferous trees,deciduous trees, and all trees using ecological drivers (soil, climate, fire, and topography) and pre-fire remote sensing predictors. Relationships between ecological drivers of post-fire vegetation trajectories were also evaluated. Additional analyses were conducted to (1) assess whether seedlings could be detected by Landsat or synthetic aperture radar (SAR) time-series analysis (2) assess pre-fire and post-fire Landsat variables against pre-fire and post-fire tree cover estimates to evaluate whether magnitude of forest change can be detected. Understanding variables that influence vegetative recovery, vegetation type conversion, and watershed characteristics will aid forest restoration efforts and water quality management.

Eric Jensen

Validation of Fitness for Duty Standards Using Pre- and Post-Flight Capsule Egress and Suited Functional Performance Tasks in Simulated Reduced Gravity (Pilot Egress Fitness)

As NASA prepares for exploration missions, it will be critical to understand and build upon known crew capabilities. Specifically, we need to know the functional capacities of crew at different stages of a mission and characterize these profiles so that the appropriate vehicle requirements and mission concepts of operations (ConOps) can be developed. One of the most complex phases of any missions is when there is a transition between gravity environments. For instance, both physiological adaptation to microgravity and subsequent re-entry into a gravity environment result in reduced functional capacity, even with rigorous adherence to inflight countermeasures. Quantification of the astronauts’ post-landing functional performance is necessary to design ConOps for exploration missions. Specifically, these two high-risk tasks may have to be performed soon after gravity transitions: •Unassisted capsule egress task after return to Earth •Planetary extravehicular activity (EVA) soon after landing on Mars or the Moon This study has been broken down into two phases. A pilot phase to assess the overall feasibility and demonstrate the capability to do these tasks shortly after landing and the full Egress Fitness study, which will be part of the CIPHER complement. This study uses a functional task approach to characterize performance of these high-risk tasks in long-duration ISS crewmembers before flight and shortly after return to Earth. Prior to any testing, each astronaut subject completes a suit fit check to ensure adequate sizing and mobility to be able to complete the EVA tasks. Pilot Egress Fitness pre-flight and post-flight testing includes an Earth based emergency egress out of a functional capsule mockup, and a short Mars gravity EVA simulation including suit donning, hatch egress, ladder descent, task board cable operations, baggage transfer over sand/rocky regolith, alignment with a rear entry port, and suit egress. Pre-flight testing can occur almost anytime pre-flight, but post-flight scheduling is much more critical with the capsule egress test occurring 1-4 hours after landing and the planetary EVA approximately 18-36 hours after landing. Data collected for both tasks includes task completion time, photo, and video. The EVA portion also includes collection of metabolic and heart rate. Pilot Egress Fitness study has completed baseline pre-flight testing on 3 astronaut subjects. Post-flight testing of the first two subjects will be completed in November 2021 and the final subject around March 2022. Results and lessons learned from this pilot study will inform the full Egress Fitness study, which will incorporate additional pre-flight sessions, longer EVA tasks, and post-flight testing on R+1, 4, and 7 to characterize the timeframe of recovery

J R Norcross

Investigation of Cyrene organosolv fractionation of softwood biomass and alkaline post-incubation

Cyrene organosolv fractionation effectively extracted 78% of lignin from recalcitrant softwood biomass pine at a mild temperature of 120°C. However, the enzymatic conversion of the fractionated cellulose-rich solid did not improve significantly. Alkali post-incubation of the fractionated cellulose fraction notably enhanced the glucan conversion. This phenomenon has also been observed in other organosolv processes, but how this approach transforms pretreated biomass has not yet been comprehensively investigated. In this study, small-angle X-ray scattering (SAXS) was employed to understand the structural changes in biomass during fractionation and post-incubation at the nanometer scale. Further, no lignin aggregation was found on the microfibrils, whereas the distance between the microfibrils increased after pretreatment and decreased after alkaline post-incubation. These results suggests that the Cyrene molecules remained between the microfibrils and were removed by post-incubation. In addition, the pine lignin recovered after pretreatment was characterized by NMR to understand the impact of Cyrene pretreatment on the lignin structure.

09 BIOMASS FUELS

Nonlinearity of the post-spinel transition and its expression in slabs and plumes worldwide

Phase transitions in the mantle control its internal dynamics and structure. The post-spinel transition marks the upper–lower mantle boundary, where ringwoodite dissociates into bridgmanite plus ferropericlase, and its Clapeyron slope regulates mantle flow across it. This interaction has previously been assumed to have no lateral spatial variations, based on the assumption of a linear post-spinel boundary in pressure and temperature. Here we present laser-heated diamond anvil cell experiments with synchrotron X-ray diffraction to better constrain this boundary, especially at higher temperatures. Combining our data with results from the literature, and using a global analysis based on machine learning, we find a pronounced nonlinearity in the post-spinel boundary, with its slope ranging from –4 MPa/K at 2100 K, to –2 MPa/K at 1950 K, and to 0 MPa/K at 1600 K. Changes in temperature over time and space can therefore cause the post-spinel transition to have variable effects on mantle convection and the movement of subducting slabs and upwelling plumes.

58 GEOSCIENCES

Post-Flight Evaluation of PICA and PICA-X - Comparisons of the Stardust SRC and Space-X Dragon 1 Forebody Heatshield Materials

Phenolic Impregnated Carbon Ablator (PICA) was developed at NASA Ames Research Center. As a thermal protection material, PICA has the advantages of being able to withstand high heat fluxes with a relatively low density. This ablative material was used as the forebody heat shield material for the Stardust sample return capsule, which re-entered the Earths atmosphere in 2006. Based on PICA, SpaceX developed a variant, PICA-X, and used it as the heat shield material for its Dragon spacecraft, which successfully orbited the Earth and re-entered the atmosphere during the COTS Demo Flight 1 in 2010. Post-flight analysis was previously performed on the Stardust PICA heat shield material. Similarly, a near-stagnation core was obtained from the post-flight Dragon 1 heat shield, which was retrieved from the Pacific Ocean. Materials testing and analyses were performed on the core to evaluate its ablation performance and post-flight properties. Comparisons between PICA and PICA-X are made where applicable. Stardust and Dragon offer rare opportunities to evaluate materials post-flight - this data is beneficial in understanding material performance and also improves modeling capabilities.

PICA-X

Altered post-fracture systemic bone loss in a mouse model of osteocyte dysfunction

Femur fracture leads to loss of bone at uninjured skeletal sites, which may increase risk of subsequent fracture. Osteocytes, the most abundant bone cells, can directly resorb bone matrix and regulate osteoclast and osteoblast activity, but their role in systemic bone loss after fracture remains poorly understood. In this study we used a transgenic (TG+) mouse model that overexpresses human B-cell lymphoma 2 (BCL-2) in osteoblasts and osteocytes. This causes enhanced osteoblast proliferation, followed by disruption in lacunar-canalicular connectivity and massive osteocyte death by 10 wk of age. We hypothesized that reduced viable osteocyte density would decrease the magnitude of systemic bone loss after femur fracture, reduce perilacunar remodeling, and alter callus formation. Bone remodeling was assessed using serum biomarkers of bone formation and resorption at 5 d post-fracture. We used micro-computed tomography, high resolution x-ray microscopy, mechanical testing, and Raman spectroscopy to quantify the magnitude of systemic bone loss, as well as changes in osteocyte lacunar volume, bone strength, and bone composition 2 wk post-fracture. Fracture was associated with a reduction in circulating markers of bone resorption in non-transgenic (TG-) animals. TG+ mice exhibited high bone mass in the limbs, greater cortical elastic modulus and reduced post-yield displacement. After fracture, TG+ mice lost less trabecular bone than TG- mice, but conversely TG+ mice exhibited trends toward a lower yield point and reduced femoral cortical thickness after fracture, though these were not statistically significant. Lacunar density was greater in TG+ mice, but fracture did not alter lacunar volume in TG+ or TG- mice. These findings suggest that osteocytes potentially play a significant role in the post-traumatic systemic response to fracture, though the effects differ between trabecular and cortical bone.

60 APPLIED LIFE SCIENCES

Chelator-mediated Fenton post-treatment enhances methane yield from lignocellulosic residues via microbial community modulation

Advancing biomethane production from anaerobic digestion (AD) is essential for building a more reliable and resilient bioenergy system. However, incomplete conversion of lignocellulose-rich agricultural waste remains a key limitation, often leaving energy-dense residues in the digestate by-product. In this study, we introduce a novel application of chelator-mediated Fenton (CMF) post-treatment to recover untapped biomethane potential from these recalcitrant residues, representing a significant departure from conventional pre-treatment strategies. By systematically varying pH, iron-chelator concentration, and hydrogen peroxide dosage, we identified reaction conditions (pH 6–8, 5 mM Fe 2+ -dihydroxybenzene, 3–4 wt.% H 2 O 2 ) that enhanced lignocellulose deconstruction and increased dissolved organic carbon (DOC) availability for methanogenesis. CMF post-treatment led to up to a tenfold increase in biomethane potential compared to untreated controls. Microbial community analysis revealed enrichment of cellulolytic species, suggesting enhanced hydrolytic activity as a driver of improved conversion. Application of the CMF post-treatment method to isolated poplar lignin further demonstrated its versatility for diverse lignocellulosic substrates. These findings position CMF post-treatment as a promising strategy to enhance AD efficiency and valorize digestate.

Martinez, Daniella Victoria [Sandia National Labor

Dissection of Carbon and Nitrogen Cycling in Post-Fire Soil Environments using a Genome- Informed Experimental Community (Final Technical Report)

Wildfires are a natural part of many forest ecosystems, with globally important carbon (C) storage and nutrient cycling consequences, and they are increasing in frequency and severity in Western North America. Forest fires affect soil C stocks in complex ways; some C is released into the atmosphere through combustion, while a large percentage of the C is added to the soil in the form of pyrogenic organic matter. Worldwide, it is estimated that 16% of soil organic matter is pyrogenic, while locally, this number may be as high as 80%. Understanding how wildfires affect soil organic matter cycling requires understanding how microbes respond to pyrogenic organic matter and other post-fire soil conditions. However, our understanding of microbial interactions within post-fire soil was in its infancy at the time of our proposal. Outstanding questions included: Which microbes are capable of degrading pyrogenic organic matter? What are the relevant genes and metabolites associated with this degradation? What are the key interactions among post-fire microbes? Key highlights of outcomes supported by this grant included training eleven early-career scientists and two early-career PIs, publication of twelve peer-reviewed papers, cross-lab collaborations that empowered complex scientific approaches, the development of an open-source automated gas sampler to drive novel insights in C cycling, enhanced understanding of post-fire microbial community dynamics, and novel genetic and molecular insights into microbial responses to fire.

54 ENVIRONMENTAL SCIENCES

Exploiting symmetries for efficient post-buckling analysis of composite plates

Two aspects of the post-buckling analysis of composite plates are considered in this paper. The first pertains to identifying the different types of symmetry exhibited by the pre- and post-buckling responses of rectangular and skewed composite plates. A procedure is presented for exploiting these symmetries in finite-element and finite-difference analyses. The procedure can be used with existing programs having multipoint constraint capability. The second aspect pertains to the post-buckling response of biaxially loaded composite plates. Numerical results from finite-element analysis are presented which show the range of parameters for which shear deformation and/or anisotropy are important in buckling and post-buckling analysis.

Noor, A. K.

Calculation of flow about posts and powerhead model

A three dimensional analysis of the non-uniform flow around the liquid oxygen (LOX) posts in the Space Shuttle Main Engine (SSME) powerhead was performed to determine possible factors contributing to the failure of the posts. Also performed was three dimensional numerical fluid flow analysis of the high pressure fuel turbopump (HPFTP) exhaust system, consisting of the turnaround duct (TAD), two-duct hot gas manifold (HGM), and the Version B transfer ducts. The analysis was conducted in the following manner: (1) modeling the flow around a single and small clusters (2 to 10) of posts; (2) modeling the velocity field in the cross plane; and (3) modeling the entire flow region with a three dimensional network type model. Shear stress functions which will permit viscous analysis without requiring excessive numbers of computational grid points were developed. These wall functions, laminar and turbulent, have been compared to standard Blasius solutions and are directly applicable to the cylinder in cross flow class of problems to which the LOX post problem belongs.

Anderson, P. G.

Post and a random-walk search mode

Multidisciplinary analysis often requires optimization of nonlinear systems that are subject to constraints. Trajectory optimization is one example of this situation. The Program to Optimize Simulated Trajectories (POST) was used successfully for a number of problems. The purpose is to describe POST and a new optimization approach that has been incorporated into it. Typical uses of POST will also be illustrated. The projected-gradient approach to optimization is the preferred option in POST and is discussed. A new approach to optimization, the random-walk approach, is described, and results with the random-walk approach are presented.

Martin, J. A.

SSME LOX post flow analyses/fluid structure interaction

Test firings of the Space Shuttle Main Engine (SSME) over the course of its development have resulted in several catastrophic failures. Some of these failures were due to breakage of the LOX posts that feed liquid oxygen into the main injector head of the engine. The objective was to perform a computational fluid flow analysis of a simplified SSME LOX post array using an existing viscous Navier-Stokes flow solver. A two-dimensional analysis was to be made. In addition, a finite element theory for treating the fluid/structure interaction between the posts and the hot gas environment was to be developed. The methodology used in computing the flow field through the LOX post array is described. Results are presented and discussed.

Source record

Post clamp

A pair of spaced collars are mounted at right angles on a clamp body by retaining rings which enable the collars to rotate with respect to the clamp body. Mounting posts extend through aligned holes in the collars and clamp body. Each collar can be clamped onto the inserted post while the clamp body remains free to rotate about the post and collar. The clamp body is selectively clamped onto each post.

Ramsey, John K.

Long duration exposure facility post-flight thermal analysis, part 1

Results of the post-flight thermal analysis of the Long Duration Exposure Facility (LDEF) mission are presented. The LDEF mission thermal analysis was verified by comparing the thermal model results to flight data from the LDEF Thermal Measurements System (THERM). Post-flight calculated temperature uncertainties have been reduced to under +/- 18 F from the pre-flight uncertainties of +/- 40 F. The THERM consisted of eight temperature sensors, a shared tape recorder, a standard LDEF flight battery, and an electronics control box. The temperatures were measured at selected locations on the LDEF structure interior during the first 390 days of flight and recorded for post-flight analysis. After the LDEF retrieval from Space on 12 Jan. 1990, the tape recorder was recovered from the spacecraft and the data reduced for comparison to the LDEF predicted temperatures. The LDEF mission temperatures were calculated prior to the LDEF deployment on 7 Apr. 1980, and updated after the LDEF retrieval with the following actual flight parameter data: including thermal fluxes, spacecraft attitudes, thermal coatings degradation, and contamination effects. All updated data used for the calculation of post-flight temperatures is also presented in this document.

Berrios, William M.

Long duration exposure facility post-flight thermal analysis, part 2

Results of the post-flight thermal analysis for the Long Duration Exposure Facility (LDEF) mission are presented. The LDEF mission thermal analysis was verified by comparing the thermal model results to flight data from the LDEF Thermal Measurements System (THERM). Post-flight calculated temperature uncertainties have been reduced to under +/- 18 F from the pre-flight uncertainties of +/- 40 F. The THERM consisted of eight temperature sensors, a shared tape recorder, a standard LDEF flight battery, and an electronics control box. The temperatures were measured at selected locations on the LDEF structure interior during the first 390 days of flight and recorded for post-flight analysis. After the LDEF retrieval from Space on 12 Jan. 1990, the tape recorder was recovered from the spacecraft and the data reduced for comparison to the LDEF predicted temperatures. The LDEF mission temperatures were calculated prior to the LDEF deployment on 7 Apr. 1980, and updated after the LDEF retrieval with the following actual flight parameter data: thermal fluxes, spacecraft attitudes, thermal coatings degradation, and contamination effects. All updated data used for calculation of post-flight temperatures is also presented in this document.

Berrios, William M.

Tensoral: A system for post-processing turbulence simulation data

Many computer simulations in engineering and science -- and especially in computational fluid dynamics (CFD) -- produce huge quantities of numerical data. These data are often so large as to make even relatively simple post-processing of this data unwieldy. The data, once computed and quality-assured, is most likely analyzed by only a few people. As a result, much useful numerical data is under-utilized. Since future state-of-the-art simulations will produce even larger datasets, will use more complex flow geometries, and will be performed on more complex supercomputers, data management issues will become increasingly cumbersome. My goal is to provide software which will automate the present and future task of managing and post-processing large turbulence datasets. My research has focused on the development of these software tools -- specifically, through the development of a very high-level language called 'Tensoral'. The ultimate goal of Tensoral is to convert high-level mathematical expressions (tensor algebra, calculus, and statistics) into efficient low-level programs which numerically calculate these expressions given simulation datasets. This approach to the database and post-processing problem has several advantages. Using Tensoral the numerical and data management details of a simulation are shielded from the concerns of the end user. This shielding is carried out without sacrificing post-processor efficiency and robustness. Another advantage of Tensoral is that its very high-level nature lends itself to portability across a wide variety of computing (and supercomputing) platforms. This is especially important considering the rapidity of changes in supercomputing hardware.

Dresselhaus, Eliot