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At least 73 records · Page 4

New Opportunities for Neutrons in Environmental and Biological Sciences

The use of neutron methods in environmental and biological sciences is rapidly emerging and accelerating with the development of new instruments at neutron user facilities. This article, based on a workshop held at Oak Ridge National Laboratory (ORNL), offers insights into the application of neutron techniques in environmental and biological sciences. Here we highlight recent advances and identify key challenges and potential future research areas. These include soil and rhizosphere processes, root water dynamics, plant-microbe interactions, structure and dynamics of biological systems, applications in synthetic biology and enzyme engineering, next-generation bioproducts, biomaterials and bioenergy, nanoscale structure, and fluid dynamics of porous materials in geochemistry. We provide an outlook on emerging opportunities with an emphasis on new capabilities that will be enabled at the Spallation Neutron Source Second Target Station currently under design at ORNL. The mission of scientific neutron user facilities worldwide is to enable science using state-of-the-art neutron capabilities. We aim to encourage researchers in the environmental and biological research community to explore the unique capability afforded by neutrons at these facilities.

54 ENVIRONMENTAL SCIENCES↗

RhizoNet segments plant roots to assess biomass and growth for enabling self-driving labs

Abstract Flatbed scanners are commonly used for root analysis, but typical manual segmentation methods are time-consuming and prone to errors, especially in large-scale, multi-plant studies. Furthermore, the complex nature of root structures combined with noisy backgrounds in images complicates automated analysis. Addressing these challenges, this article introduces RhizoNet, a deep learning-based workflow to semantically segment plant root scans. Utilizing a sophisticated Residual U-Net architecture, RhizoNet enhances prediction accuracy and employs a convex hull operation for delineation of the primary root component. Its main objective is to accurately segment root biomass and monitor its growth over time. RhizoNet processes color scans of plants grown in a hydroponic system known as EcoFAB, subjected to specific nutritional treatments. The root detection model using RhizoNet demonstrates strong generalization in the validation tests of all experiments despite variable treatments. The main contributions are the standardization of root segmentation and phenotyping, systematic and accelerated analysis of thousands of images, significantly aiding in the precise assessment of root growth dynamics under varying plant conditions, and offering a path toward self-driving labs.

59 BASIC BIOLOGICAL SCIENCES↗

Chaotic roots of the modular multiplication dynamical system in Shor's algorithm

Shor's factoring algorithm, believed to provide an exponential speedup over classical computation, relies on finding the period of an exactly periodic quantum modular multiplication operator. This exact periodicity is the hallmark of an integrable system, which is paradoxical from the viewpoint of quantum chaos, given that the classical limit of the modular multiplication operator is a highly chaotic system that occupies the “maximally random” Bernoulli level of the classical ergodic hierarchy. In this work, we approach this apparent paradox from a quantum dynamical systems viewpoint, and consider whether signatures of ergodicity and chaos may indeed be encoded in such an “integrable” quantization of a chaotic system. We show that Shor's modular multiplication operator, in specific cases, can be written as a superposition of quantized 𝐴-baker's maps exhibiting more typical signatures of quantum chaos and ergodicity. This work suggests that the integrability of Shor's modular multiplication operator may stem from the interference of other “chaotic” quantizations of the same family of maps, and paves the way for deeper studies on the interplay of integrability, ergodicity, and chaos in and via quantum algorithms.

Dynamical systems↗

Fine-root functional trait responses to experimental warming: a global meta-analysis

Whether and how warming alters functional traits of absorptive plant roots remains to be answered across the globe. Tackling this question is crucial to better understanding terrestrial responses to climate change as fine-root traits drive many ecosystem processes. In this work, we carried out a detailed synthesis of fine-root trait responses to experimental warming by performing a meta-analysis of 964 paired observations from 177 publications. Warming increased fine-root biomass, production, respiration and nitrogen concentration as well as decreased root carbon : nitrogen ratio and nonstructural carbohydrates. Warming effects on fine-root biomass decreased with greater warming magnitude, especially in short-term experiments. Furthermore, the positive effect of warming on fine-root biomass was strongest in deeper soil horizons and in colder and drier regions. Total fine-root length, morphology, mortality, life span and turnover were unresponsive to warming. Our results highlight the significant changes in fine-root traits in response to warming as well as the importance of warming magnitude and duration in understanding fine-root responses. These changes have strong implications for global soil carbon stocks in a warmer world associated with increased root-derived carbon inputs into deeper soil horizons and increases in fine-root respiration.

59 BASIC BIOLOGICAL SCIENCES↗

A Methodology for Robust Load Reduction in Wind Turbine Blades Using Flow Control Devices

Decades of wind turbine research, development and installation have demonstrated reductions in levelized cost of energy (LCOE) resulting from turbines with larger rotor diameters and increased hub heights. Further reductions in LCOE by up-scaling turbine size can be challenged by practical limitations such as the square-cube law: where the power scales with the square of the blade length and the added mass scales with the volume (the cube). Active blade load control can disrupt this trend, allowing longer blades with less mass. This paper presents the details of the development of a robust load control system to reduce blade fatigue loads. The control system, which we coined sectional lift control or SLC, uses a lift actuator model to emulate an active flow control device. The main contributions of this paper are: (1) Methodology for SLC design to reduce dynamic blade root moments in a neighborhood of the rotor angular frequency (1P). (2) Analysis and numerical evidence supporting the use of a single robust SLC for all wind speeds, without the need for scheduling on wind speed or readily available measurements such as collective pitch or generator angular speed. (3) Intuition and numerical evidence to demonstrate that the SLC and the turbine controller do not interact. (4) Evaluation of the SLC using a full suite of fatigue and turbine performance metrics.

17 WIND ENERGY↗

Results of buffet tests in a cryogenic wind tunnel

Buffet tests on two semispan wing models with different leading edge sweep show that it is feasibile to use the standard dynamic wing root bending moment technique in a cryogenic wind tunnel. One model was a slender 65 deg swept delta wing with sharp leading edges. The other model was an unswept wing of aspect ratio 1.5 with a British NPL 9510 airfoil section. The results for the 65 deg swept delta wing indicate the importance of matching the reduced frequency parameter in model tests for planforms which are sensitive to reduced frequency parameter if quantitative buffet measurements are required. The unique ability of a pressurized cryogenic wind tunnel to separate the effects of Reynolds number and of static aeroelastic distortion by variations in the tunnel stagnation temperature and pressure were demonstrated.

Boyden, R. P.↗

Viscous effects on transonic airfoil stability and response

Viscous effects on transonic airfoil stability and response are investigated using an integral boundary layer model coupled to the inviscid XTRAN2L transonic small disturbance code. Unsteady transonic airloads required for stability analyses are computed using a pulse transfer function analysis including viscous effects. The pulse analysis provides unsteady aerodynamic forces for a wide range of reduced frequency in a single flow field computation. Nonlinear time marching aeroelastic solutions are presented which show the effects of viscosity on airfoil response behavior and flutter. Effects of amplitude on time marching responses are demonstrated. A state space aeroelastic model employing Pade approximants to describe the unsteady airloads is used to study the effects of viscosity on transonic airfoil stability. State space dynamic pressure root loci are in good overall agreement with time marching damping and frequency estimates. Parallel sets of results with and without viscous effects reveal the effects of viscosity on transonic unsteady airloads and aeroelastic characteristics of airfoils.

Berry, H. M.↗

Viscous effects on transonic airfoil stability and response

An integral boundary layer model coupled to the inviscid XTRAN2L transonic small disturbance code is used to investigate viscous effects on transonic airfoil stability and response. A pulse transfer function analysis is employed for the computation of unsteady transonic airloads which yields forces for a wide range of reduced frequencies in a single flowfield computation. Nonlinear, time-marching aeroelastic solutions are obtained which exhibit the effects of viscosity on airfoil response behavior and flutter. A state-space aeroelastic model using Pade approximants to describe unsteady airloads is used to study viscosity effects on transonic airfoil stability. The state-space dynamic pressure root loci are in good general agreement with time marching damping and frequency estimates.

Berry, H. M.↗

Slowly moving thermal features on Jupiter

Maps of the Jovian upper troposphere temperatures derived from Voyager 1 and Voyager 2 infrared interferometer spectrometer (IRIS) spectra show large-scale features which move very slowly. The motion is very different from the inferred local fluid velocity. It is suggested that these features may indicate a deeply-rooted fluid dynamical regime beneath the surface meteorology.

Magalhaes, J. A.↗

Tail Lobe Revisited: Magnetic Field Modeling Based on Plasma Data

Plasma data from the ISEE-1 and -2 spacecraft during 1977-1980 have been used to determine the distribution of data points in the magnetotail in the range of distances -20 < XGSM < --15, i.e. which of the records that were located in the current sheet, in the tail lobe, in the magnetosheath and in the boundary layers respectively. The ISEE-1 and -2 magnetic field data for the records in the tail lobe were then used to model the tail lobe magnetic field dependence on the solar wind dynamic pressure, on the Interplanetary Magnetic Field (IMF) and on the Dst index. The tail lobe magnetic field was assumed to be dependent on the square root of the dynamic pressure based on the balance between the total magnetic pressure in the tail lobes and the dynamic pressure of the solar wind. The IMF dependent terms, added to the pressure term, were sought in many different forms while the Dst dependence of the tail lobe magnetic field was assumed to be linear. The field shows a strong dependence on the square root of the dynamic pressure and the different IMF dependent terms all constitute a significant contribution to the total field. However, the dependence on the Dst index turned out to be very weak at those down-tail distances. The results of this study are intended to be used for parameterizing future versions of the data-based models of the global magnetospheric magnetic field.

Karlsson, S. B. P.↗

Genetic ablation of root cap cells in Arabidopsis

The root cap is increasingly appreciated as a complex and dynamic plant organ. Root caps sense and transmit environmental signals, synthesize and secrete small molecules and macromolecules, and in some species shed metabolically active cells. However, it is not known whether root caps are essential for normal shoot and root development. We report the identification of a root cap-specific promoter and describe its use to genetically ablate root caps by directing root cap-specific expression of a diphtheria toxin A-chain gene. Transgenic toxin-expressing plants are viable and have normal aerial parts but agravitropic roots, implying loss of root cap function. Several cell layers are missing from the transgenic root caps, and the remaining cells are abnormal. Although the radial organization of the roots is normal in toxin-expressing plants, the root tips have fewer cytoplasmically dense cells than do wild-type root tips, suggesting that root meristematic activity is lower in transgenic than in wild-type plants. The roots of transgenic plants have more lateral roots and these are, in turn, more highly branched than those of wild-type plants. Thus, root cap ablation alters root architecture both by inhibiting root meristematic activity and by stimulating lateral root initiation. These observations imply that the root caps contain essential components of the signaling system that determines root architecture.

Non-NASA Center↗

Spatial variation of corn canopy temperature as dependent upon soil texture and crop rooting characteristics

A soil plant atmosphere model for corn (Zea mays L.) together with the scaling theory for soil hydraulic heterogeneity are used to study the sensitivity of spatial variation of canopy temperature to field averaged soil texture and crop rooting characteristics. The soil plant atmosphere model explicitly solves a continuity equation for water flux resulting from root water uptake, changes in plant water storage and transpirational flux. Dynamical equations for root zone soil water potential and the plant water storage models the progressive drying of soil, and day time dehydration and night time hydration of the crop. The statistic of scaling parameter which describes the spatial variation of soil hydraulic conductivity and matric potential is assumed to be independent of soil texture class. The field averaged soil hydraulic characteristics are chosen to be representative of loamy sand and clay loam soils. Two rooting characteristics are chosen, one shallow and the other deep rooted. The simulation shows that the range of canopy temperatures in the clayey soil is less than 1K, but for the sandy soil the range is about 2.5 and 5.0 K, respectively, for the shallow and deep rooted crops.

Choudhury, B. J.↗

Dynamic tooth loads and stressing for high contact ratio spur gears

An analysis and computer program were developed for calculating the dynamic gear tooth loading and root stressing for high contact ratio gearing (HCRG) as well as LCRG. The analysis includes the effects of the variable tooth stiffness during the mesh, tooth profile modification, and gear errors. The calculation of the tooth root stressing caused by the dynamic gear tooth loads is based on a modified Heywood gear tooth stress analysis, which appears more universally applicable to both LCRG and HCRG. The computer program is presently being expanded to calculate the tooth contact stressing and PV values. Sample application of the gear program to equivalent LCRG (1.566 contact ratio) and HCRG (2.40 contact ratio) revealed the following: (1) the operating conditions and dynamic characteristics of the gear system an affect the gear tooth loading and root stressing, and therefore, life significantly; (2) the length of the profile modification affect the tooth loading and root stressing significantly, the amount depending on the applied load, speed, and contact ratio; and (3) the effect of variable tooth stiffness is small, shifting and increasing the response peaks slightly from those for constant tooth stiffness.

Cornell, R. W.↗

Multiple-orifice liquid injection into hypersonic airstreams and applications to ram C-3 flight

Experimental data are presented for the oblique injection of water and three electrophilic liquids (fluorocarbon compounds) through multiple-orifice nozzles from a flat plate and the sides of a hemisphere-cone (0.375 scale of RAM C spacecraft) into hypersonic airstreams. The nozzle patterns included single and multiple orifices, single rows of nozzles, and duplicates of the RAM C-III nozzles. The flat-plate tests were made at Mach 8. Total pressure was varied from 3.45 MN/m2 to 10.34 MN/m2, Reynolds number was varied form 9,840,000 per meter to 19,700,000 per meter, and liquid injection pressure was varied from 0.69 MN/m2 to 3.5 MN/m2. The hemisphere-cone tests were made at Mach 7.3. Total pressure was varied from 1.38 MN/m2, to 6.89 MN/m2, Reynolds number was varied from 3,540,000 per meter to 17,700,000 per meter, and liquid-injection pressure was varied from 0.34 MN/m2 to 4.14 MN/m2. Photographs of the tests and plots of liquid-penetration and spray cross-section area are presented. Maximum penetration was found to vary as the square root of the dynamic-pressure ratio and the square root of the total injection nozzle area. Spray cross-section area was linear with maximum penetration. The test results are used to compute injection parameters for the RAM C-3 flight injection experiment.

Weaver, W. L.↗

Consequences of Altered Root Nutrient Uptake for Soil Carbon Stabilization (Final Report)

Objectives: The primary objectives of this project are to improve our understanding of tropical forest belowground processes, and to increase representation of this biome in ecosystem-scale and global C cycle models. Belowground dynamics present a major source of uncertainty inhibiting our ability to predict C cycle responses to climate change. Representation of tropical forests in these models is particularly lacking, even though these ecosystems hold >25% of terrestrial C stocks. While the majority of humid tropical forests exist on relatively infertile soils (i.e. poor in rock-derived nutrients), enormous gradients in soil nutrient availability exist at landscape-scales due to shifts in geology and soil order. Soil fertility is likely to greatly influence how tropical soil C storage will respond to the declines in precipitation predicted for the tropics. In particular, root dynamics vary across soil fertility gradients, with roots representing the major input of C to subsurface soils. Root characteristics related to soil fertility include biomass, turnover, C exudates, tissue chemistry, and nutrient uptake rates, with each of these likely sensitive to changes in moisture. The proposed project will fully integrate field research with an existing ecosystem model to assess potential effects of drying on belowground tropical C dynamics across a range of soil fertilities. Project Description:. This project will identify key linkages among soil fertility, root nutrient uptake, root dynamics, and soil C storage. The hypothesized framework for understanding these linkages is: Tropical rainforest roots in relatively fertile soils have lesser root biomass, faster turnover, fewer exudates, and improved root tissue quality relative to infertile sites, all resulting from increased root nutrient uptake. Ultimately, these root characteristics in fertile soils promote proportionally greater long-term soil C storage in organo-mineral associations. Specifically, lower root exudates and improved root tissue quality in fertile soils reduce microbial respiration of extant soil C (i.e. “priming) and increase microbial C use efficiency, leading to sorption of protein-rich microbial and plant organic matter on soil mineral surfaces. However, the larger pools of mineral-associated C in fertile tropical soils are more vulnerable to decreased rainfall than smaller C pools in infertile soils, in part because of the lesser root biomass, faster turnover, and softer, more labile root tissues. Also, the shallower lateral rooting structures typical in fertile soils experience greater desiccation and death than deeper roots in infertile sites, with little short-term adaptation to drying.

54 ENVIRONMENTAL SCIENCES↗

Bacterial Community Members Increase Bacillus subtilis Maintenance on the Roots of Arabidopsis thaliana

Plant-growth-promoting bacteria (PGPB) are used to improve plant health and promote crop production. However, because some PGPB (including Bacillus subtilis) do not maintain substantial colonization on plant roots over time, it is unclear how effective PGPB are throughout the plant growing cycle. A better understanding of the dynamics of plant root community assembly is needed to develop and harness the potential of PGPB. Although B. subtilis is often a member of the root microbiome, it does not efficiently monoassociate with plant roots. We hypothesized that B. subtilis may require other primary colonizers to efficiently associate with plant roots. We utilized a previously designed hydroponic system to add bacteria to Arabidopsis thaliana roots and monitor their attachment over time. We inoculated seedlings with B. subtilis and individual bacterial isolates from the native A. thaliana root microbiome either alone or together. We then measured how the coinoculum affected the ability of B. subtilis to colonize and maintain on A. thaliana roots. We screened 96 fully genome-sequenced strains and identified five bacterial strains that were able to significantly improve the maintenance of B. subtilis. Three of these rhizobacteria also increased the maintenance of two strains of B. amyloliquefaciens commonly used in commercially available bioadditives. These results not only illustrate the utility of this model system to address questions about plant–microbe interactions and how other bacteria affect the ability of PGPB to maintain their relationships with plant roots but also may help inform future agricultural interventions to increase crop yields.

59 BASIC BIOLOGICAL SCIENCES↗

Completing below-ground carbon budgets for pastures, recovering forests, and mature forests of Amazonia

This progress report covers the following efforts initiated for the year: year-round monthly soil CO2 flux measurements were started in both primary and secondary forests and in managed and degraded pastures; root sorting and weighing has begun and all four ecosystems at Paragominas have been analyzed through samples; regional modeling of soil water dynamics and minimum rooting depth has been done and the RADAMBRASIL soils database has been digitized and a 20 year record of the precipitation for the region has been produced, along with a hydrological ('bucket-tipping') model that will run within a GIS framework; prototype tension lysimeters have been designed and installed in soil pits to begin assessing the importance of DOC as a source of organic matter in deep soils; and many publications, listed in this document, have resulted from this year's research. Two of the papers published are included with this annual report document.

Davidson, Eric A.↗

Root architectural plasticity optimizes nutrient acquisition in switchgrass under variable phosphorus forms

Aims Understanding the influence of different forms of phosphorus (P) over the different root traits and how those traits are related to increasing the efficiency of nutrient acquisition strategies. Methods Investigation of switchgrass (Panicum virgatum L.) root morphology responses to inorganic P (Pi) soluble (Potassium-P), insoluble (Aluminum-P), and organic P (Po) (Inositol Hexa-Phosphate, IHex-P) in rhizoboxes. Roots were traced over the root box and scanned using WinRhizoTM. The CRootbox model was employed to simulate root growth. Results Significant plasticity observed under IHex-P treatment, with a 46% increase in root branching, leading to a 74% rise in total root length and a 65% increase in root surface area compared to inorganic P forms. IHex-P resulted in a 73% higher root biomass than Aluminum-P and a 26% increase compared to Potassium-P. Most of the differences were attributed to the elongation of root branches. Conclusions Here, the study emphasizes the dynamic nature of switchgrass root architecture and morphology in response to varying P forms in the soil. The absence of Pi in the soil triggered increased plasticity in root traits, facilitating root access to Po and uptake of P. These findings offer valuable insights into the adaptive mechanisms of perennial plants, with significant implications for optimizing nutrient acquisition strategies in both agricultural and natural ecosystems.

Organic phosphorus↗