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Cooling rate calculations for silicate glasses

The effects of thermal properties, sample size, and quench medium on cooling rates achieved during quenching are evaluated for a variety of samples with different thermal properties. It is found that, depending on the composition and the property being observed, quench rate may or may not be fast enough to preserve a high-temperature property. If the cooling rate dependence of a property in a given system is unknown, then the cooling rate must be specified so that others may duplicate specific conditions. The effect of cooling rate can be experimentally tested by manipulation of sample size and quench medium. These results should add to understanding of glass-forming phenomena on the moon.

Birnie, D. P., III↗

Space Flown Rodent Liver RNA Sequencing Data for Machine Learning in Space Biology Research

High-throughput nucleic acid sequencing (DNA-seq, RNA-seq) has become widespread in biomedical research due to the growing availability and affordability of these assays. Data analysis has been accelerated in recent years by the adoption of artificial intelligence (AI) and machine learning (ML) techniques by biomedical researchers. In space biology research, RNAseq datasets from space-flown experimental samples are critical for characterizing the gene expression aberrations associated with exposure to spaceflight stressors. However, space biological experiments tend to be very low sample size, so identifying proper AI/ML algorithms for sequencing data analysis is an ongoing challenge since these algorithms typically require large sample size. The NASA Science Mission Directorate (SMD) has started the “Benchmark Initiative for AI/ML”, focused on creating datasets meant for three main applications: 1) scientific benchmarking, which finds the best algorithm for a specific problem; 2) application benchmarking, which measures algorithm performance against a set of parameters; and 3) system benchmarking, which evaluates performance of hardware and software architecture. These scientific benchmarks consist of an AI-ready dataset and a reference implementation on a specific scientific question. In this work, we focused on generating standardized datasets to allow the scientific community to benchmark AI/ML algorithms in the domain of space biology. We present here a standardized, AI-ready, publicly available benchmark dataset for space biology RNA-seq data as a collaboration between the NASA AI4LS (Artificial Intelligence for Life Sciences) working group. and NASA’s SMD. This dataset consists of space-flown and ground control mouse liver found in the NASA GeneLab omics database. However, to amplify the small sample number (n=112 samples) for ML purposes, we employ Gaussian noise and a generative adversarial network to extend this dataset to 6,000 synthetic samples, matching the original gene expression characteristics.

James Casaletto↗

Robustness-Based Design Optimization Under Data Uncertainty

This paper proposes formulations and algorithms for design optimization under both aleatory (i.e., natural or physical variability) and epistemic uncertainty (i.e., imprecise probabilistic information), from the perspective of system robustness. The proposed formulations deal with epistemic uncertainty arising from both sparse and interval data without any assumption about the probability distributions of the random variables. A decoupled approach is proposed in this paper to un-nest the robustness-based design from the analysis of non-design epistemic variables to achieve computational efficiency. The proposed methods are illustrated for the upper stage design problem of a two-stage-to-orbit (TSTO) vehicle, where the information on the random design inputs are only available as sparse point and/or interval data. As collecting more data reduces uncertainty but increases cost, the effect of sample size on the optimality and robustness of the solution is also studied. A method is developed to determine the optimal sample size for sparse point data that leads to the solutions of the design problem that are least sensitive to variations in the input random variables.

Zaman, Kais↗

Efficacy of FTIR Analysis in Determining CO2 Loading on Diglycolamine

In support of advanced air revitalization technologies to enable human spaceflight beyond low earth orbit, performance studies have been conducted using a liquid amine, Diglycolamine (DGA) between teams at NASA's Johnson Spaceflight Center (JSC) and Ames Research Center (ARC). Liquid amines have been used in regenerable earth-based systems to remove CO2 from industrial systems as well as for closed-environment air revitalization because they can be regenerated at lower temperatures than solid sorbent systems. As an additional advantage to solid sorbent-based systems, liquid sorbents can be cycled between an adsorbing contactor and degassing chamber, thereby reducing system complexity by operation in a continuous loop. In an effort to inform a regeneration system design for micro-gravity applications, ARC has performed a number of tests to characterize the degas mechanics of DGA. In order to accurately measure the amount of CO2 captured or released by the amine, methods such as gravimetric weighing and chemical desorption are reasonable, however the first iteration test setup for a scaled down degas system required analysis on small sample sizes. Fourier-transform infrared spectroscopy (FTIR) analysis was experimentally evaluated to analyze CO2 concentration because it can produce measurements with sample sizes on the order of 100's of μL. Calibration against chemical desorption showed relatively good correlation and test data showed reasonable adherence to expected trends, however more extensive testing should be conducted to fully validate the usage of FTIR to determine CO2 loading on DGA.

Diglycolamine↗

Pre-Mission Input Requirements to Enable Successful Sample Collection by a Remote Field/EVA Team

This paper is intended to evaluate the sample collection process with respect to sample characterization and decision making. In some cases, it may be sufficient to know whether a given outcrop or hand sample is the same as or different from previous sampling localities or samples. In other cases, it may be important to have more in-depth characterization of the sample, such as basic composition, mineralogy, and petrology, in order to effectively identify the best sample. Contextual field observations, in situ/handheld analysis, and backroom evaluation may all play a role in understanding field lithologies and their importance for return. For example, whether a rock is a breccia or a clast-laden impact melt may be difficult based on a single sample, but becomes clear as exploration of a field site puts it into context. The FINESSE (Field Investigations to Enable Solar System Science and Exploration) team is a new activity focused on a science and exploration field based research program aimed at generating strategic knowledge in preparation for the human and robotic exploration of the Moon, near-Earth asteroids (NEAs) and Phobos and Deimos. We used the FINESSE field excursion to the West Clearwater Lake Impact structure (WCIS) as an opportunity to test factors related to sampling decisions. In contract to other technology-driven NASA analog studies, The FINESSE WCIS activity is science-focused, and moreover, is sampling-focused, with the explicit intent to return the best samples for geochronology studies in the laboratory. This specific objective effectively reduces the number of variables in the goals of the field test and enables a more controlled investigation of the role of the crewmember in selecting samples. We formulated one hypothesis to test: that providing details regarding the analytical fate of the samples (e.g. geochronology, XRF/XRD, etc.) to the crew prior to their traverse will result in samples that are more likely to meet specific analytical objectives than samples collected in the absence of this premission information. We conducted three tests of this hypothesis. Our investigation was designed to document processes, tools and procedures for crew sampling of planetary targets. This is not meant to be a blind, controlled test of crew efficacy, but rather an effort to recognize the relevant variables that enter into sampling protocol and to develop recommendations for crew and backroom training in future endeavors. Methods: One of the primary FINESSE field deployment objectives was to collect impact melt rocks and impact melt-bearing breccias from a number of locations around the WCIS structure to enable high precision geochronology of the crater to be performed [1]. We conducted three tests at WCIS after two full days of team participation in field site activities, including using remote sensing data and geologic maps, hiking overland to become familiar with the terrain, and examining previously-collected samples from other islands. In addition, the team members shared their projects and techniques with the entire team. We chose our "crew members" as volunteers from the team, all of whom had had moderate training in geologic fieldwork and became familiar with the general field setting. The first two tests were short, focused tests of our hypothesis. Test A was to obtain hydrothermal vugs; Test B was to obtain impact melt and intrusive rock as well as the contact between the two to check for contact metamorphism and age differences. In both cases, the test director had prior knowledge of the site geology and had developed a study-specific objective for sampling prior to deployment. Prior to the field deployment, the crewmember was briefed on the sampling objective and the laboratory techniques that would be used on the samples. At the field sites (Fig. 2), the crewmember was given 30 minutes to survey a small section of outcrop (10-15 m) and acquire a suite of three samples. The crewmember talked through his process and the test director kept track of the timeline in verbal cues to the crewmember. At the conclusion, the team member conducting the scientific study appraised the samples and train of thought. Test C was a 90-minute EVA simulation using two crewmembers working out of line-of-sight in communication with a science backroom. The science objectives were determined by the science backroom team in advance using a Gigapan image of the outcrop (Fig. 1). The science team formulated hypotheses for the outcrop units and created sampling objectives for impact-melt lithologies; the science team turned these into a science plan, which they communicated to the crew in camp prior to crew deployment. As part of the science plan, the science team also discussed their sample needs in depth with the crewmembers, including laboratory methods, objectives, and samples sizes needed. During the deployment, the two crewmembers relayed real-time information to the science backroom by radio with no time delay. Both the crew and science team re-evaluated their hypotheses and science plans in real-time. Discussion: Upon evaluation, we found that the focused tests (Tests A and B) were successful in meeting their scientific objectives. The crewmember used their knowledge of how the samples were to be used in further study (technique, sample size, and scientific need) to focus on the sampling task. The crewmember was comfortable spending minimal time describing and mapping the outcrop. The crewmember used all available time to get a good sample. The larger test was unsuccessful in meeting the sampling objectives. When the crewmembers began describing the lithologies, it was quickly apparent that the lithologies were not as the backroom expected and had communicated to the crew. When the outcrop wasn't as expected, the crew members instinctively switched to field characterization mode, taking significant time to characterize and map the outcrop. One crew member admitted that he "kind of lost track" of the sampling strategy as he focused on the basic outcrop characterization. This is the logical first step in a field geology campaign, that a significant amount of time must be spent by the crew and backroom to understand the outcrop and its significance. Basic field characterization of an outcrop is a focused activity that takes significant time and training [2, 3]. Sampling of representational lithologies can be added to this activity for little cost [4]. However, we have shown that identification of unusual or specific samples for laboratory study also takes significant time and knowledge. We suggest that sampling of this type be considered a separate activity from field characterization, and that crewmembers be trained in sampling needs for different kinds of studies (representative lithologies vs. specialized samples) to acquire a mindset for sampling similar to field mapping. Sampling activities should be given a significant amount of specifically allocated time in scheduling EVA activities; and in the better case, that sampling be done as a second activity to a previously studied outcrop where both crew and backroom are comfortable with its context and characteristics. Our hypothesis posited that crewmember knowledge of how the samples would be used upon return would aid them in choosing relevant samples. Our testing bore this hypothesis out to some extent. We therefore recommend that crewmember training should include exposure to the laboratory techniques and analyses that will be used on the samples to foster this knowledge. There is also the potential for increasing crewmember contextual knowledge real-time in the field through the introduction of in situ geochemical technologies such as field portable XRF. The presence of field portable geochemical technology could enable the astronauts to interrogate the samples for K abundance real-time, ensuring they could collect valuable and dateable samples [5]. Though simulations such as these can teach us a fair bit about decision making processes and timeline building, one EVA participant noted that when he wasn't collecting "real" samples, he wasn't at his best. This effect suggests that higher-fidelity studies involving truly remote participants conducting actual scientific studies merit further attention to capture lessons for application to future crew situations.

sample collection↗

HYPERSAMP

Hypergeometric Attribute Sampling System Based on Risk and Fraction Defective (HYPERSAMP) computer program demonstrates attribute sampling system developed to determine minimum sample size required for any preselected value for consumer's risk and fraction of nonconforming units. Used in place of MIL-STD-105E sampling plans when minimum sample size desirable, such as when tests are destructive or expensive. Written for IBM PC-compatible computers.

Desalvo, Louis J.↗

A 2-D Piston Effect Solution for the Relaxation in the MISTE Cell

When a large density stratification is no longer a problem in a microgravity environment, one would like to increase the sample size in order to increase the signal-to-noise ratio for a specific heat measurement. To reduce the equilibration time associated with the large sample size, we designed a cylindrical cell containing a stack of plates that separate the bulk fluid into 60 equally thin layers. To understand the thermal behavior of the whole cell, we analyzed the thermal behavior of a 2-D composite system of a cylindrical near-critical fluid layer in contact with a cylindrical copper plate. In this 2-D analysis, the circumference boundary of the two cylindrical layers is subjected to a step temperature change. The solution of this 2-D composite system includes the piston effect that speeds up the equilibration in the near-critical fluid layer and the pure diffusion in the copper plate. The results of this analysis indicate that the characteristic length for the equilibration of the stacked cell is determined by an effective thickness of a single fluid layer instead of the total height of the cylindrical cell.

Weilert, Mark↗

Salvaging Data Records with Missing Data: Data Imputation using the Multivariate t Distribution

When doing multivariate data analysis, one commonobstacle is the presence of incomplete observations, i.e., observationsfor which one or more key fields are blank. Missing datais often countered by deleting entire observations that containmissing data. The negative effects of deleting entire observationsare multiple: deleting observations reduces sample size andcan also result in biased inferences even if data is missing atrandom. In addition, knowledge contained within incompleteobservations is knowledge lost when they are deleted– and theeffort spent collecting that knowledge is effort wasted. Data imputationmethods, or methods of statistically “filling-in” missingdata, can help combat small sample sizes by using the existinginformation in partially complete observations with the end goalof producing less biased and higher confidence inferences. Whena sample from a multivariate normal population is only partiallycomplete, and the missing data meets appropriate assumptions(missing at random), robust data imputation of the missing datacan be implemented with monotone data augmentation (MDA)using the multivariate t distribution.Missing data imputation is applied to data from the NASA InstrumentCost Model (NICM) using the MDA algorithm underthe assumption of having a multivariate t distribution with fixeddegrees of freedom. A sensitivity analysis to the degrees offreedom parameter is presented to demonstrate robustness ofthe multivariate t distribution when dealing with small samplesas compared to the multivariate normal distribution.

DiNicola, Michael↗

Exploring the Relationship Between Science Funding and Science Productivity on Extended Missions

Introduction: The goal of this summer internship research project was to explore the relationship between the level of funding for science team research and science productivity for planetary spaceflight missions. The productivity and funding for the prime and extended missions were compared when possible. Determining the actual rather than anecdotal relationship between the level of science team funding and their productivity will help determine the adequacy of science team funding. Additionally, determining whether productivity increases or decreases over time (within missions, or within the years between different missions) provides insights into ongoing trends. This project was conducted using data from 11 extended missions within the Planetary Science Division. The main goals of this project were to 1) quantify the level of productivity of the science team during the extended portion of each mission, 2) compare the level of productivity between different types of missions, and 3) compare the level of productivity between the prime missions and extended missions. Methodology: The central metric enters around the “publication rate” of each mission. This rate is normalized in a variety of ways, including the number of publications per year, per Co-I, and per instruments. For the most part, this data was attainable through mission websites, team rosters, and data collected from previous projects. The publication data for this project utilized the SAO/NASA Astrophysics Data System, and it consisted of a search for any papers from the extended mission that had a co-investigator as an author. The time period for these searches also included an additional two years after the end of the extended mission to account for additional time it typically takes to finish data analysis and publish papers. Data from prime missions was collected from a previous project that measured similar metrics for the same missions. When comparing the different types of missions, three factors were used: mission size, dates of the prime and extended missions, and the type of the mission (rover, flyby, orbiter, or lander). To determine mission size, missions were compared by the number of Co-I’s on the mission, the number of instruments in the payload, and the mission class. Findings: On average, prime mission publication rates were generally higher than those of extended missions. Out of the nine missions used for this comparison, six had a higher prime mission publication rate and three had a higher extended mission publication rate. This distinction was interesting to observe, but it should be noted that the methodology for determing the number of published papers differed for the prime missions and the extended missions Discovery missions were found to be the most scientifically productive. Additionally, more recent extended missions tend to possess greater productivity. When comparing missions of different types, it was found that orbiters were much more productive than other types of missions. Future Work: There are a number of opportunities to extend and improve on this project in the future. Even though the metrics used were the same, the prime mission data could be recollected from the same sources as the extended mission data (Astrophysics Data System) to insure greater consistency. The impact level of the publications could also be accounted for, and financial data could be used to compare teams with different levels of funding. Additionally, more missions could be examined to increase the sample size of these comparisons, especially the comparison of different mission types which had an incredibly small sample size for some categories.

Cameron Maxwell Thompson↗

The narrow pulse approximation and long length scale determination in xenon gas diffusion NMR studies of model porous media

We report a systematic study of xenon gas diffusion NMR in simple model porous media, random packs of mono-sized glass beads, and focus on three specific areas peculiar to gas-phase diffusion. These topics are: (i) diffusion of spins on the order of the pore dimensions during the application of the diffusion encoding gradient pulses in a PGSE experiment (breakdown of the narrow pulse approximation and imperfect background gradient cancellation), (ii) the ability to derive long length scale structural information, and (iii) effects of finite sample size. We find that the time-dependent diffusion coefficient, D(t), of the imbibed xenon gas at short diffusion times in small beads is significantly affected by the gas pressure. In particular, as expected, we find smaller deviations between measured D(t) and theoretical predictions as the gas pressure is increased, resulting from reduced diffusion during the application of the gradient pulse. The deviations are then completely removed when water D(t) is observed in the same samples. The use of gas also allows us to probe D(t) over a wide range of length scales and observe the long time asymptotic limit which is proportional to the inverse tortuosity of the sample, as well as the diffusion distance where this limit takes effect (approximately 1-1.5 bead diameters). The Pade approximation can be used as a reference for expected xenon D(t) data between the short and the long time limits, allowing us to explore deviations from the expected behavior at intermediate times as a result of finite sample size effects. Finally, the application of the Pade interpolation between the long and the short time asymptotic limits yields a fitted length scale (the Pade length), which is found to be approximately 0.13b for all bead packs, where b is the bead diameter. c. 2002 Elsevier Sciences (USA).

NASA Discipline Life Sciences Technologies↗

Small-Sample Estimator Decisions – Certainly Uncertain in Human Spaceflight at NASA

Within statistics when estimating means, we rely heavily on the Central Limit Theorem (CLT) to aid in our inference, however in cases where the CLT does not apply we begin to be strongly limited in our choices. If a violation to the CLT comes in the form of small samples and unknown population variance, yet we maintain the assumption of normality, the use of t-distributions is perfectly valid. We explore cases where population normality is not assumed, where sample sizes are small, and where some actionable estimate of the central tendency of a distribution is needed such as for spaceflight operational decisions. Here we identify different scenarios based on measures taken from spaceflight, characterizing performance of estimators of central tendency within our set of simulations. We examine bias and variability of standard estimators of central tendency as they apply to varying small sample sizes and varying population distributions. Further work in this area is needed to develop a framework or set of guidelines for individuals set in these situations. Even with limited information, decisions need to be made.

Central Tendency↗

Transcriptomics-based Machine Learning Analysis Predicts Space-Exposed Murine Livers

Limited sample sizes, high data dimensionality, and sensitivity to technical and biological variability of next generation sequencing (NGS), has typically limited machine learning (ML) in space studies and further study of radiation effects. However, pooling smaller studies while addressing intra- and inter-study variabilities allows for ML predictive modeling. Here, integration methods were applied to whole transcriptome shotgun sequencing (RNAseq) data from 6 mouse liver GeneLab datasets (GLDS) with a total of 113 spaceflight and ground-control samples to determine top features relevant to spaceflight including the effect of radiation exposure. Data was normalized within each study, then merged and scaled across all datasets. Data dimensionality was reduced using a minimum redundancy maximum relevance (MRMR) methodology. The top MRMR features were used to predict spaceflight vs. ground-control samples using a Random Forest (RF) classifier with 5-fold cross validation (CV). The ML-based gene sets were further compared against differential gene expression results from individual GLDS. CV training using the top 100 MRMR genes show averages of 86% accuracy and 0.95 AUC value on the validation set over 5 folds (Figure 1A). Baseline set analysis on differentially expressed genes (DEGs) identified using padj ≤ 0.05 show 811 or 68 DEGs overlapping between at least 2 or 3 studies, respectively (Figure 1B). Over-representation analysis showed overlapping biological processes related to fatty acid and lipid metabolism. Set analysis between the MRMR features and the DEGs showed 60 or 8 genes overlapping with at least 1 or 2 studies, respectively. MRMR feature selection and ensemble ML methods (e.g. RF) improve performance relative to a Naïve Bayes classifier when NGS data sets are analyzed. A challenge of applying ML methods across heterogeneous NGS data is accounting for signal:noise ratio. Here, signal validation across studies was shown by intersecting sets between top MRMR genes and DEGs from RNASeq analysis. Non-intersecting sets introduce opportunity to explore spaceflight relevant genes and implementing ML methods across existing NGS datasets may overcome sample size limitations. ML coupled with existing analytical methods enhances understanding of disease by revealing common underlying pathways across datasets.

Machine Learning↗

Computational Bayesian Methods Applied to Complex Problems in Bio and Astro Statistics

In this dissertation we apply computational Bayesian methods to three distinct problems. In the first chapter, we address the issue of unrealistic covariance matrices used to estimate collision probabilities. We model covariance matrices with a Bayesian Normal-Inverse-Wishart model, which we fit with Gibbs sampling. In the second chapter, we are interested in determining the sample sizes necessary to achieve a particular interval width and establish non-inferiority in the analysis of prevalences using two fallible tests. To this end, we use a third order asymptotic approximation. In the third chapter, we wish to synthesize evidence across multiple domains in measurements taken longitudinally across time, featuring a substantial amount of structurally missing data, and fit the model with Hamiltonian Monte Carlo in a simulation to analyze how estimates of a parameter of interest change across sample sizes.

Elrod, Chris↗

Jitter Correction

Jitter_Correct.m is a MATLAB function that automatically measures and corrects inter-frame jitter in an image sequence to a user-specified precision. In addition, the algorithm dynamically adjusts the image sample size to increase the accuracy of the measurement. The Jitter_Correct.m function takes an image sequence with unknown frame-to-frame jitter and computes the translations of each frame (column and row, in pixels) relative to a chosen reference frame with sub-pixel accuracy. The translations are measured using a Cross Correlation Fourier transformation method in which the relative phase of the two transformed images is fit to a plane. The measured translations are then used to correct the inter-frame jitter of the image sequence. The function also dynamically expands the image sample size over which the cross-correlation is measured to increase the accuracy of the measurement. This increases the robustness of the measurement to variable magnitudes of inter-frame jitter

Waegell, Mordecai J.↗

Non-Targeted Effects and the Dose Response for Heavy Ion Tumorigenesis

BACKGROUND: There is no human epidemiology data available to estimate the heavy ion cancer risks experienced by astronauts in space. Studies of tumor induction in mice are a necessary step to estimate risks to astronauts. Previous experimental data can be better utilized to model dose response for heavy ion tumorigenesis and plan future low dose studies. DOSE RESPONSE MODELS: The Harderian Gland data of Alpen et al.[1-3] was re-analyzed [4] using non-linear least square regression. The data set measured the induction of Harderian gland tumors in mice by high-energy protons, helium, neon, iron, niobium and lanthanum with LET s ranging from 0.4 to 950 keV/micron. We were able to strengthen the individual ion models by combining data for all ions into a model that relates both radiation dose and LET for the ion to tumor prevalence. We compared models based on Targeted Effects (TE) to one motivated by Non-targeted Effects (NTE) that included a bystander term that increased tumor induction at low doses non-linearly. When comparing fitted models to the experimental data, we considered the adjusted R2, the Akaike Information Criteria (AIC), and the Bayesian Information Criteria (BIC) to test for Goodness of fit.In the adjusted R2test, the model with the highest R2values provides a better fit to the available data. In the AIC and BIC tests, the model with the smaller values of the summary value provides the better fit. The non-linear NTE models fit the combined data better than the TE models that are linear at low doses. We evaluated the differences in the relative biological effectiveness (RBE) and found the NTE model provides a higher RBE at low dose compared to the TE model. POWER ANALYSIS: The final NTE model estimates were used to simulate example data to consider the design of new experiments to detect NTE at low dose for validation. Power and sample sizes were calculated for a variety of radiation qualities including some not considered in the Harderian Gland data set and with different background tumor incidences. We considered different experimental designs with varying number of doses and varying low doses dependant on the LET of the radiation. The optimal design to detect a NTE for an individual ion had 4 doses equally spaced below a maximal dose where bending due to cell sterilization was < 2%. For example at 100 keV/micron we would irradiate at 0.03 Gy, 0.065 Gy, 0.13 Gy, and 0.26 Gy and require 850 mice including a control dose for a sensitivity to detect NTE with 80% power. Sample sizes could be improved by combining ions similar to the methods used with the Harderian Gland data.

Chappelli, Lori J.↗

Assessment of Countermeasure Efficacy for Long-Term Space Missions

One of the main functions of the upcoming International Space Station (ISS) will be to provide a venue for testing proposed countermeasures for their ability to protect humans from the debilitating effects of longterm space flight. However, several limiting factors preclude an evaluation process similar to that used in clinical trials which traditionally are implemented with large sample sizes of subjects, including control groups, and with blind or double-blind application of treatments according to factorial or other balanced experimental designs. In particular, only very limited numbers of human subjects will be available for actual field testing in the ISS With no more than 125 subjects planned to fly on all ISS missions over 10 years, it is not possible to test extensive combinations of some 15-20 proposed countermeasures. Furthermore because of safety concerns and operational considerations, it is unlikely that anything other than the current best guess at the most effective countermeasure package will ever be used on ISS. In particular, control or placebos will not be allowed. In view of these limitations, historical data and groundbased or animal studies will have to be used to compensate for small sample sizes and lack of controls in the field. As a result, statistical analysis methodology will have to be developed which allows for the integration of these disparate data types into a meaningful evaluation process. The process must be sequential, providing objective rules for deciding through time whether to reject or modify an ineffective countermeasure, or whether to certify one as effective. Additional output should include performance characteristics for all relevant physiological systems, including uncertainty analyses and estimates of accept/reject decision error rates.

Feiveson, Alan H.↗

Laser Diffraction Techniques Replace Sieving for Lunar Soil Particle Size Distribution Data

Sieving was used extensively until 1999 to determine the particle size distribution of lunar samples. This method is time-consuming, and requires more than a gram of material in order to obtain a result in which one may have confidence. This is demonstrated by the difference in geometric mean and median for samples measured by [1], in which a 14-gram sample produced a geometric mean of approx.52 micrometers, whereas two other samples of ~1.5 grams resulted in gave means of approx.63 and approx.69 micrometers. Sample allocations for sieving are typically much smaller than a gram, and many of the sample allocations received by our lab are 0.5 to 0.25 grams in mass. Basu [2] has described how the finest fraction of the soil is easily lost in the sieving process, and this effect is compounded when sample sizes are small.

Cooper, Bonnie L.↗