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At least 73 records · Page 4

Quorum sensing modulates microbial community structure through regulation of secondary metabolites

Bacteria are recognized for their diverse metabolic capabilities, yet the impact of microbe-microbe interactions on multispecies community structure and dynamics is poorly understood. Cell-to-cell signaling in the form of quorum sensing (QS) often regulates secondary metabolite production and microbial interactions. Here, we examine how acylhomoserine lactone (AHL)-mediated QS impacts microbial community structure in a 10-member synthetic community of isolates from Populus deltoides . To explore the role of QS in microbial community structure and dynamics, we disrupted AHL signaling by exogenous addition of AiiA-lactonase, an enzyme that cleaves the lactone ring. Microbial community structure resulting from signal inactivation, as measured by 16S rRNA amplicon sequencing and secondary metabolite production, was assessed after successive passaging of the community. Further, we investigated the impact of quorum quenching on specific microbe-microbe interactions using pairwise inhibition assays. Our results indicate that AHL inactivation alters the relative abundance of dominant community members at later passages but does not impact the overall membership in the community. Quorum quenching significantly alters the metabolic profile in lactonase-treated communities. This metabolic alteration impacts microbe-microbe interactions through decreased inhibition of other community members. Together, these results indicate that QS impacts microbial community structure through the regulation of secondary metabolites in dominant members and that the membership of microbial communities can be relatively stable despite changes in metabolic profiles.

59 BASIC BIOLOGICAL SCIENCES↗

A reconnaissance space sensing investigation of crustal structure for a strip from the eastern Sierra Nevada to the Colorado Plateau

The author has identified the following significant results. Studies were conducted in key field areas in the Sierra Nevada, the Basin Range Province and the Colorado Plateau to evaluate the origins and significance of geologic and structural anomalies expressed in the ERTS-1 data. The investigation included development of image enhancement and analysis techniques and comparison of remote sensing data available over the test site. The ERTS-1 MSS imagery has proven to be an effective tool for studying the interrelationsships between Cenozoic tectonic patterns and the distributions of Cenozoic plutonism and volcanism, seismic activity, geologic hazards, and known mineral, geothermal and ground water resources. Recommendations are made for applications of ERTS-1 data to natural and resource exploration and management.

Liggett, M. A.↗

System for determining the angle of impact of an object on a structure

A method for determining the angle of impact of an object on a thin-walled structure which determines the angle of impact through analysis of the acoustic waves which result when an object impacts a structure is presented. Transducers are placed on and in the surface of the structure which sense the wave caused in the structure by impact. The waves are recorded and saved for analysis. For source motion normal to the surface, the antisymmetric mode has a large amplitude while that of the symmetric mode is very small. As the source angle increases with respect to the surface normal, the symmetric mode amplitude increases while the antisymmetric mode amplitude decreases. Thus, the angle of impact is determined by measuring the relative amplitudes of these two lowest order modes.

Prosser, William H.↗

Passive Wireless Vibration Sensing for Measuring Aerospace Structural Flutter

To reduce energy consumption, emissions, and noise, NASA is exploring the use of high aspect ratio wings on subsonic aircraft. Because high aspect ratio wings are susceptible to flutter events, NASA is also investigating methods of flutter detection and suppression. In support of that work a new remote, non-contact method for measuring flutter-induced vibrations has been developed. The new sensing scheme utilizes a microwave reflectometer to monitor the reflected response from an aeroelastic structure to ultimately characterize structural vibrations. To demonstrate the ability of microwaves to detect flutter vibrations, a carbon fiber-reinforced polymer (CFRP) composite panel was vibrated at various frequencies from 1Hz to 130Hz. The reflectometer response was found to closely resemble the sinusoidal response as measured with an accelerometer up to 100 Hz. The data presented demonstrate that microwaves can be used to measure flutter-induced aircraft vibrations.

Wilson, William C.↗

Multispectral remote sensing as stratigraphic and structural tool, Wind River Basin and Big Horn Basin areas, Wyoming

The use of Landsat TM, Airborne Imaging Spectrometer, and airborne Thermal IR Multispectral Scanner data in the geological evaluation of two sites in central Wyoming is described and illustrated with diagrams, maps, photographs, sample images, and tables of numerical data. The value of the remotely sensed information on the areal variation of attitude, sequence, thickness, and lithology of exposed strata is demonstrated; details of the data analysis are given; and the specialized software packages employed are briefly characterized.

Lang, Harold R.↗

Shear Stress Sensing with Elastic Microfence Structures

In this work, elastic microfences were generated for the purpose of measuring shear forces acting on a wind tunnel model. The microfences were fabricated in a two part process involving laser ablation patterning to generate a template in a polymer film followed by soft lithography with a two-part silicone. Incorporation of a fluorescent dye was demonstrated as a method to enhance contrast between the sensing elements and the substrate. Sensing elements consisted of multiple microfences prepared at different orientations to enable determination of both shear force and directionality. Microfence arrays were integrated into an optical microscope with sub-micrometer resolution. Initial experiments were conducted on a flat plate wind tunnel model. Both image stabilization algorithms and digital image correlation were utilized to determine the amount of fence deflection as a result of airflow. Initial free jet experiments indicated that the microfences could be readily displaced and this displacement was recorded through the microscope.

Cisotto, Alexxandra↗

Mapping Pine Plantations in the Southeastern U.S. Using Structural, Spectral, and Temporal Remote Sensing Data

The southeastern U.S. produces the most industrial roundwood in the U.S. each year, largely from commercial pine plantations. The extent of plantation forests and management dynamics can be difficult to ascertain from periodic forest inventories, yet short-rotation tree plantations also present challenges for remote sensing. Here, we integrated spectral, temporal, and structural information from airborne and satellite platforms to distinguish pine plantations from natural forests and evaluate the contribution from planted forests to regional forest coverin the southeastern U.S. Within flight lines from NASA Goddard's Lidar, Hyperspectral, and Thermal (G-LiHT) Airborne Imager, lidar metrics of forest structure had the highest overall accuracy for pine plantations among single-source classifications (90%), but the combination of spectral and temporal metrics from Landsat generated comparable accuracy (91%). Combined structural, temporal, and spectral information from G-LiHT and Landsat had the highest accuracy for plantations (92%) and natural forests (88%). At a regional scale, classifications using Landsat spectral and temporal metrics had between 74 and 82% mean class accuracy for plantations.Regionally, plantations accounted for 28% of forest cover in the southeastern U.S., a result similar to plot-based estimates, albeit with greater spatial detail. Regional maps of plantation forests differed from existing map products, including the National Land Cover Database. Combining plantation extent in 2011 with Landsat based forest change data identified strong regional gradients in plantation dynamics since 1985, with distinct spatial patterns of rotation age (east-west) and plantation expansion (interior). Our analysis demonstrates the potential to improve the characterization of dynamic land cover classes, including economically important timber plantations, by integrating diverse remote sensing datasets. Critically, multi-source remote sensing provides an approach to leverage periodic forest inventory data for annual monitoring of managed forest landscapes.

Fagan, M. E.↗

Remote sensing of row crop structure and component temperatures using directional radiometric temperatures and inversion techniques

A physically based sensor response model of a row crop was used as the mathematical framework from which several inversion strategies were tested for extracting row structure information and component temperatures using a series of sensor view angles. The technique was evaluated on ground-based radiometric thermal infrared data of a cotton row crop that covered 48 percent of the ground in the vertical projection. The results showed that the accuracies of the predicted row heights and widths, vegetation temperatures, and soil temperatures of the cotton row crop were on the order of 5 cm, 1 deg, and 2 deg C, respectively. The inversion techniques can be applied to directional sensor data from aircraft platforms and even space platforms if the effects of atmospheric absorption and emission can be corrected. In theory, such inversion techniques can be applied to a wide variety of vegetation types and thus can have significant implications for remote sensing research and applications in disciplines that deal with incomplete vegetation canopies.

Kimes, D. S.↗

Method and structure for minimizing error sources in image and position sensing detectors

A method and structure for minimizing one or more non-uniformities in image and position sensing detectors are provided. The structure is directed to a focal plane processor for removing non-uniformities which distort the computation of a desired property of an object of interest in an image field. The focal plane processor is capable of selectively disconnecting one or more rows and/or columns from further processing in the imaging array for those rows and/or columns which contribute to the presence of at least one non-uniformity in a video image generated by the focal plane processor. In one embodiment, the disconnection means is embodied as pre-processing circuitry which includes row and column shift registers which provide control signals to area-of-interest (AOI) switches. In another embodiment, the pixels which comprise the focal plane array are constructed in a manner which facilitates their individual isolation.

Strohbehn, Kim↗

A reconnaissance space sensing investigation of crustal structure for a strip from the eastern Sierra Nevada to the Colorado Plateau

There are no author-identified significant results in this report. Research progress in applications of ERTS-1 MSS imagery in study of Basin-Range tectonics is summarized. Field reconnaissance of ERTS-1 image anomalies has resulted in recognition of previously unreported fault zones and regional structural control of volcanic and plutonic activity. NIMBUS, Apollo 9, X-15, U-2, and SLAR imagery are discussed with specific applications, and methods of image enhancement and analysis employed in the research are summarized. Areas studied and methods employed in geologic field work are outlined.

Bechtold, I. C.↗

A reconnaissance space sensing investigation of crustal structure for a strip from the eastern Sierra Nevada to the Colorado Plateau

The author has identified the following significant results. Analysis of ERTS-1 MSS imagery over the sourthern Basin-Range Province of California, Nevada, and Arizona has led to recognition of regional tectonic control of volcanism, plutonism, mineralization, and fault patterns. This conclusion is the result of geologic reconnaissance of anomalies observed in ERTS-1 and Apollo-9 data, guided by intermediate scale U-2 photography, SLAR, and relevant geologic literature. In addition to regional tectonic studies, the ERTS-1 imagery provides a basis for detailed research of relatively small geologic features. Interpretation of ERTS-1 and Apollo-9 space imagery and intermediate scale X-15 and U-2 photography indicates the presence of a major fault zone along the California-Nevada state line, here named the Pahrump fault zone. Field mapping confirms previously unreported evidence of fault breaks in bedrock, along range fronts and in Quaternary alluvium and lake sediments. Regional gravity lows and fault traces within the Pahrump fault zone from a general left stepping en echelon pattern. The trend and postulated diplacement for this fault are similar to other major strike slip fault zones in the southern Basin-Range Province.

Bechtold, I. C.↗

A reconnaissance space sensing investigation of crustal structure for a strip from the eastern Sierra Nevada to the Colorado Plateau

The author has identified the following significant results. Research progress in applications of ERTS-1 MSS imagery to study of Basin-Range tectonics is summarized. Field reconnaissance of ERTS-1 image anomalies has resulted in recognition of previously unreported fault zones and regional structural control of volcanic and plutonic activity. Nimbus, Apollo 9, X-15, U-2, and SIAR imagery are discussed with specific applications, and methods of image enhancement and analysis employed in the research are summarized. Field areas studied and methods employed in geologic field work are outlined.

Liggett, M. A.↗

A reconnaissance space sensing investigation of crustal structure for a strip from the eastern Sierra Nevada to the Colorado Plateau

The author has identified the following significant results. An operational technique for producing high resolution false color composites of ERTS-1 MSS imagery has been developed. The technique uses standard NASA (NDPF) or EROS black/white data products, and permits a broad range of control on composite color balance and contrast range. The use of an additive color viewer has proven valuable for determining the optimum color balance of composites for effective interpretation. The compositing technique is adaptable for effective interpretation. The technique is adaptable to data from a variety of multispectral imaging systems, including multi-seasonal and multipolarization data. A 50 mile north-trending linear anomaly recognized in ERTS-1 MSS imagery west of Goldfield, Nevada has been confirmed as a steeply west-dipping normal fault, here termed the Paymaster Fault. This structure trends southward from the General Thomas Hills along Paymaster Valley and the east side of Clayton Valley into the Palmetto Mountains. There, it terminates against a less pronounced east-trending transverse anomaly which is also believed to be fault controlled. The Paymaster Fault is the most prominent of several large normal faults which terminate southward against this transverse anomaly.

Liggett, M. A.↗

A reconnaissance space sensing investigation of crustal structure for a strip from the eastern Sierra Nevada to the Colorado Plateau

The author has identified the following significant results. Research progress in an investigation using ERTS-1 MSS imagery to study regional tectonics and related natural resources is summarized. Field reconnaissance guided by analysis of ERTS-1 imagery has resulted in development of a tectonic model relating strike-slip faulting to crustal extension in the southern Basin Range Province. The tectonics of the northern Death Valley-Furnace Creek Fault Zone and spacially associated volcanism and mercury mineralization were also investigated. Field work in the southern Sierra Nevada has confirmed the existence of faults and diabase dike swarms aligned along several major lineaments first recognized in ERTS-1 imagery. Various image enhancement and analysis techniques employed in the study of ERTS-1 data are summarized.

Liggett, M. A.↗

Carbon Nanotube-based Sensor and Method for Continually Sensing Changes in a Structure

A sensor has a plurality of carbon nanotube (CNT)-based conductors operatively positioned on a substrate. The conductors are arranged side-by-side, such as in a substantially parallel relationship to one another. At least one pair of spaced-apart electrodes is coupled to opposing ends of the conductors. A portion of each of the conductors spanning between each pair of electrodes comprises a plurality of carbon nanotubes arranged end-to-end and substantially aligned along an axis. Because a direct correlation exists between resistance of a carbon nanotube and carbon nanotube strain, changes experienced by the portion of the structure to which the sensor is coupled induce a change in electrical properties of the conductors.

Jordan, Jeffry D.↗