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DOE OSTI2024

An engineered lactate oxidase based electrochemical sensor for continuous detection of biomarker lactic acid in human sweat and serum

Lactate levels in humans reveal intensity and duration of exertion and provide a critical readout for the severity of life-threatening illnesses such as pediatric sepsis. Using the lactate oxidase enzyme (Lox) from Aerococcus viridians, we demonstrated its functionality for lactate electrochemical sensing in physiological fluids in a lab setting. The structure and dynamics of LOx were validated by crystallography, X-ray scattering, and hydroxyl radical protein footprinting. This provided a validated protein template for understanding and designing an enzyme-based electrochemical sensing elements. Using this template, LOx enzyme variants were generated and compared. Comparison of the variants demonstrates that one exhibits effective lactate sensing at significantly reduced operating voltages. Additionally, we demonstrate that the four hexahistidine-tags on each enzyme tetramer are sufficient for immobilization to create a durable, functional sensor, with no need for a covalent attachment, enabling self-immobilization and eliminating the need for additional immobilization steps. The functionality of the LOx enzyme variants was verified at physiological lactate concentrations in both human serum (0–4 mM) and artificial sweat (0–100 mM) using 3-electrode setups for analysis of the three variants in parallel. Accuracy of measurement in both artificial sweat and human serum were high. Employing a microfluidic flow cell, we successfully monitored varying lactate levels in physiological fluids continuously over a 2h period. Overall, this optimized LOx enzyme, which self-immobilizes onto gold sensing electrodes, facilitates efficient and reliable lactate detection and continuous monitoring at reduced operating voltages suitable for further development towards commercial use.

60 APPLIED LIFE SCIENCES↗

NASA NTRS1992

Controlled ecological life-support system - Use of plants for human life-support in space

Scientists and engineers within NASA are conducting research which will lead to development of advanced life-support systems that utilize higher plants in a unique approach to solving long-term life-support problems in space. This biological solution to life-support, Controlled Ecological Life-Support System (CELSS), is a complex, extensively controlled, bioengineered system that relies on plants to provide the principal elements from gas exchange and food production to potable water reclamation. Research at John F. Kennedy Space Center (KSC) is proceeding with a comprehensive investigation of the individual parts of the CELSS system at a one-person scale in an approach called the Breadboard Project. Concurrently a relatively new NASA sponsored research effort is investigating plant growth and metabolism in microgravity, innovative hydroponic nutrient delivery systems, and use of highly efficient light emitting diodes for artificial plant illumination.

Chamberland, D.↗

NASA NTRS2013

Precursor Asteroid Missions and Synergies to Human Exploration of Phobos and Deimos

U.S. President Obama stated on April 15, 2010 that the next goal for human spaceflight will be to send human beings to a near-Earth asteroid by 2025 and then on to the Martian system in the 2030s. Given this direction from the White House, NASA has been involved in studying various strategies for near-Earth object (NEO) exploration in order to follow U.S. space exploration policy. These missions would be the first human expeditions to interplanetary bodies beyond the Earth-Moon system and would prove useful for testing technologies required for human missions to Mars and its moons, as well as other Solar System destinations. Robotic precursor missions to NEOs would undoubtedly provide a great deal of technical and engineering data on spacecraft operations for future human space exploration while conducting in-depth scientific investigations of these primitive objects. In addition, the resulting scientific investigations would refine designs for future extraterrestrial resource extraction and utilization, which may play a vital role in leveraging potential resources from the Martian moons that in turn could enable robotic and human exploration of Mars.

Abell, Paul↗

NASA NTRSDate not supplied

Surviving and Thriving in Space and on Earth's Oceans - Human Logistics and Sustainability Comparisons and Considerations

The human species has a yearning for exploration as evidenced by the extensive historical ocean voyages and expeditions which have led to a massive advancement in the scientific and geodetic knowledge about planet Earth. These global explorations via the oceans have also had strategic, economic, cultural and religious implications and impacts, which have drastically changed the state of humanity and its condition. The transportation network created by ships traveling across the oceans has been supplemented by other transportation networks on land and in the air, creating a global economy that, in general, has improved the human condition leading to better health, longer lives, lower child mortality, better education, political freedom, higher gross national product (GNP) and improved hygiene. The logical extension of this societal trend is to extend the transportation network and human civilization into outer space, beyond the cradle of planet Earth. Our solar system contains vast amounts of natural resources which can be harnessed and used to bootstrap a space economy and related infrastructure by using advanced technologies. Sailing journeys from hundreds of years ago required large vessels and large crews, (in comparison with today’s space capsules). Modern sailors of today are able to complete large voyages, in small vessels, with a minimal crew, comparable in magnitude to modern space travel. This paper will use a systems engineering approach (e.g. using the NASA Human Integration Design Handbook (HIDH), NASA-SP-2010-3407, 2010 and the “Advanced Life Support Baseline Values and Assumptions Document, (BVAD)” NASA-CR-2004-208941, 2004.), to examine and compare the logistics and sustainability aspects of a small crew traveling on Earth's oceans in sailing vessels versus humans traveling in space. The “Mālama Honua Worldwide Voyage” of the Hokule’a, a replica of an ancient Hawaiian double hulled sailing canoe, will be used as a case study. This is the best comparison case since the Polynesian exploration of the vast (and virtually empty) Pacific Ocean is the closest analogue to modern space travel. “"Both are voyages of exploration.” –Shuttle astronaut Lacy Veach. Minimizing waste and maximizing re-use and re-cycling will lead to more efficient logistics and sustainability. In addition, In-Situ Resource Utilization (ISRU) strategies, based on successful Earth based strategies used for many years by sailors will be considered and evaluated for their usefulness. For example, human logistics for a typical space mission are shown in Table 1 and Table 2 (Lopez et al, 2015). Studies show that typical human water consumption in space is projected to be 3.2 kg/day per crew member as shown in Table 2. Data from human sailing voyages around the globe will be examined. Anecdotal evidence indicates that knowledgeable and well-equipped modern sailors, who conserve water, can comfortably live using 1.5 to 5 kg/day per person. This paper will investigate key logistics and sustainability aspects of living in space and compare them quantitatively to similar aspects of living on ocean faring sailing vessels on Earth. Mutually beneficial observations, advanced technologies and modern considerations will be applied within confines of a remote sailing environment, which could be of immense value to both the space faring community and the ocean sailing community.

Space↗

NASA NTRS1998

Methodology for Examining the Operator and the System Concurrently: Pilot Interaction with Automation

Complex system description is problematic when considering operator task activities interacting with system dynamics. Engineering languages have matured sufficiently to allow machine system description at various levels of depth and breadth but without operator synergy. Concurrently, Task Analysis methods have evolved along diverse lines enabling a description of the operator in the system from various paradigms but not describing the system. A void exists when attempting to view the system and the operator in the same plane. We propose a methodology employing descriptive languages from different domains viewed in a single dimension. Finite Automata (FA) languages describe the machine system in the proposed approach. Operator task specifications, a form of task analysis output, examine the operator activities within the system. Operator task specifications were then selected for discrete task activities and overlaid on the system description to examine operator inputs and subsequently view system responses. Unexpected (surprise) and undesirable system behavior was expected to emerge from this analysis. In this paper we shall first describe the methodology and show how the two perspectives, machine model and operator task specifications are integrated. Following we describe the process of doing such analysis using an example from cockpit automation. The methodology was employed in the analysis of a new function that was added to an existing automatic flight control system. We begin by defining a flight scenario involving all aspects of pilot interaction with the new function. Then, we proceed to develop a basic model of the machine behavior, in the context of pilot actions. Finally we superimpose the operator task specification on the machine model and perform the analysis. The proposed methodology may have broad appeal to system designers and human factors specialists. A common language for engineers of diverse domains is a strong point of this approach. Systems engineers may not fully understand operational considerations and human limitations, and human factors specialists may not be exposed to the full extent of system behavior. We hope the proposed methodology is adopted by both disciplines and in the process each assimilates a common language to address potential shortcomings of either approach separately.

Austin, David↗

NASA NTRS2006

Dawn of a New Space Age: Developing a Global Exploration Strategy.

Jeff Volosin is an aerospace engineer with over 20 years of experience in the design, development, and operations of both robotic and crewed spacecraft. Mr. Volosin is currently leading the NASA effort to develop and integrate a global exploration strategy which reflects the lunar exploration interests of international space agencies, academia and commercial stakeholders. Prior to joining NASA as a member of the Exploration Systems Mission Directorate in 2004, Jeff was an aerospace contractor, serving in a number of leadership positions including: Operations Manager for the NASA Communications Network and Flight Operations Manager for the Advanced Composition Explorer, Tropical Rainfall Measuring Mission, and the NOAA Polar and Geostationary satellite constellations. Earlier in his career, Jeff spent 4 years as a system engineer supporting the Space Exploration Initiative studies on human voyages to the Moon and Mars and also supported the Space Station program as an advanced life support engineer.

Volosin, Jeff↗

NASA NTRS2007

Development of the J-2X Engine for the Ares I Crew Launch Vehicle and the Ares V Cargo Launch Vehicle: Building on the Apollo Program for Lunar Return Missions

The United States (U.S.) Vision for Space Exploration has directed NASA to develop two new launch vehicles for sending humans to the Moon, Mars, and beyond. In January 2006, NASA streamlined its hardware development approach for replacing the Space Shuttle after it is retired in 2010. Benefits of this approach include reduced programmatic and technical risks and the potential to return to the Moon by 2020 by developing the Ares I Crew Launch Vehicle (CLV) propulsion elements now, with full extensibility to future Ares V Cargo Launch Vehicle (CaLV) lunar systems. The Constellation Program selected the Pratt & Whitney Rocketdyne J-2X engine to power the Ares I Upper Stage Element and the Ares V Earth Departure Stage (EDS). This decision was reached during the Exploration Systems Architecture Study and confirmed after the Exploration Launch Projects Office performed a variety of risk analyses, commonality assessments, and trade studies. This paper narrates the evolution of that decision; describes the performance capabilities expected of the J-2X design, including potential commonality challenges and opportunities between the Ares I and Ares V launch vehicles; and provides a current status of J-2X design, development, and hardware testing activities. This paper also explains how the J-2X engine effort mitigates risk by testing existing engine hardware and designs; building on the Apollo Program (1961 to 1975), the Space Shuttle Program (1972 to 2010); and consulting with Apollo era experts to derive other lessons learned to deliver a human-rated engine that is on an aggressive development schedule, with its first demonstration flight in 2012.

Greene, WIlliam↗

NASA NTRS2007

Development of the J-2X Engine for the Ares I Crew Launch Vehicle and the Ares V Cargo Launch Vehicle: Building on the Apollo Program for Lunar Return Missions

The United States (U.S.) Vision for Space Exploration has directed NASA to develop two new launch vehicles for sending humans to the Moon, Mars, and beyond. In January 2006, NASA streamlined its hardware development approach for replacing the Space Shuttle after it is retired in 2010. Benefits of this approach include reduced programmatic and technical risks and the potential to return to the Moon by 2020, by developing the Ares I Crew Launch Vehicle (CLV) propulsion elements now, with full extensibility to future Ares V Cargo Launch Vehicle (CaLV) lunar systems. The Constellation Program selected the Pratt & Whitney Rocketdyne J-2X engine to power the Ares I Upper Stage Element and the Ares V Earth Departure Stage. This decision was reached during the Exploration Systems Architecture Study and confirmed after the Exploration Launch Projects Office performed a variety of risk analyses, commonality assessments, and trade studies. This paper narrates the evolution of that decision; describes the performance capabilities expected of the J-2X design, including potential commonality challenges and opportunities between the Ares I and Ares V launch vehicles; and provides a current status of J-2X design, development, and hardware testing activities. This paper also explains how the J-2X engine effort mitigates risk by testing existing engine hardware and designs; building on the Apollo Program (1961 to 1975), the Space Shuttle Program (1972 to 2010); and consulting with Apollo-era experts to derive other lessons lived to deliver a human-rated engine that is on an aggressive development schedule, with its first demonstration flight in 2012.

Greene, William D.↗

NASA NTRS2013

Near-Earth Object Exploration and Marco Polo-R

Abell, Paul↗

NASA NTRS2013

Exploration of Near-Earth Asteroids

A major goal for NASA's human spaceflight program is to send astronauts to near-Earth asteroids (NEAs) in the coming decades. Missions to NEAs would undoubtedly provide a great deal of technical and engineering data on spacecraft operations for future human space exploration while conducting in-depth scientific examinations of these primitive objects. However, prior to sending human explorers to NEAs, robotic investigations of these bodies would be required in order to maximize operational efficiency and reduce mission risk. These precursor missions to NEAs would fill crucial strategic knowledge gaps concerning their physical characteristics that are relevant for human exploration of these relatively unknown destinations. Information obtained from a human investigation of a NEA, together with ground-based observations and prior spacecraft investigations of asteroids and comets, will also provide a real measure of ground truth to data obtained from terrestrial meteorite collections. Major advances in the areas of geochemistry, impact history, thermal history, isotope analyses, mineralogy, space weathering, formation ages, thermal inertias, volatile content, source regions, solar system formation, etc. can be expected from human NEA missions. Samples directly returned from a primitive body would lead to the same kind of breakthroughs for understanding NEAs that the Apollo samples provided for understanding the Earth-Moon system and its formation history. In addition, robotic precursor and human exploration missions to NEAs would allow the NASA and its international partners to gain operational experience in performing complex tasks (e.g., sample collection, deployment of payloads, retrieval of payloads, etc.) with crew, robots, and spacecraft under microgravity conditions at or near the surface of a small body. This would provide an important synergy between the worldwide Science and Exploration communities, which will be crucial for development of future international deep space exploration architectures and has potential benefits for future exploration of other destinations beyond low-Earth orbit.

Abell, Paul↗

NASA NTRS2013

Human Exploration of Near-Earth Asteroids

A major goal for NASA's human spaceflight program is to send astronauts to near-Earth asteroids (NEA) in the coming decades. Missions to NEAs would undoubtedly provide a great deal of technical and engineering data on spacecraft operations for future human space exploration while conducting in-depth scientific examinations of these primitive objects. However, before sending human explorers to NEAs, robotic investigations of these bodies would be required to maximize operational efficiency and reduce mission risk. These precursor missions to NEAs would fill crucial strategic knowledge gaps concerning their physical characteristics that are relevant for human exploration of these relatively unknown destinations. Dr. Paul Abell discussed some of the physical characteristics of NEOs that will be relevant for EVA considerations, reviewed the current data from previous NEA missions (e.g., Near-Earth Asteroid Rendezvous (NEAR) Shoemaker and Hayabusa), and discussed why future robotic and human missions to NEAs are important from space exploration and planetary defense perspectives.

Abell, Paul↗

NASA NTRS2013

Human Exploration of Near-Earth Asteroids

Abell, Paul↗

NASA NTRS2016

Misconceptions of Synthetic Biology: Lessons from an Interdisciplinary Summer School

In 2014, an international group of scholars from various fields analysed the "societal dimensions" of synthetic biology in an interdisciplinary summer school. Here, we report and discuss the biologists' observations on the general perception of synthetic biology by non-biologists who took part in this event. Most attendees mainly associated synthetic biology with contributions from the best-known public figures of the field, rarely mentioning other scientists. Media extrapolations of those contributions appeared to have created unrealistic expectations and irrelevant fears that were widely disconnected from the current research in synthetic biology. Another observation was that when debating developments in synthetic biology, semantics strongly mattered: depending on the terms used to present an application of synthetic biology, attendees reacted in radically different ways. For example, using the term "GMOs" (genetically modified organisms) rather than the term "genetic engineering" led to very different reactions. Stimulating debates also happened with participants having unanticipated points of view, for instance biocentrist ethicists who argued that engineered microbes should not be used for human purposes. Another communication challenge emerged from the connotations and inaccuracies surrounding the word "life", which impaired constructive debates, thus leading to misconceptions about the abilities of scientists to engineer or even create living organisms. Finally, it appeared that synthetic biologists tend to overestimate the knowledge of non-biologists, further affecting communication. The motivation and ability of synthetic biologists to communicate their work outside their research field needs to be fostered, notably towards policymakers who need a more accurate and technical understanding of the field to make informed decisions. Interdisciplinary events gathering scholars working in and around synthetic biology are an effective tool in addressing those issues.

synthetic biology↗

NASA NTRSDate not supplied

BioNutrients-2: Improvements to the BioNutrients-1 Nutrient Production System

The BioNutrients (BN) project aims to develop an on-demand biological production system capable of rapid and safe delivery of multiple nutrients in single-use production packs for long-duration missions. The general concept of this system is to develop edible engineered microorganisms that produce nutrients for human consumption following long-term storage, revival, and growth. BN-1 is a five-year flight project currently on the International Space Station (ISS). It is testing the long-term storage of various microorganisms for the biomanufacturing of space-relevant compounds and validating the performance of the first generation of production packs. The BN-2 project expands the BN-1 flight project scope by introducing novel products, broadening the range of microbial food sources, and improving upon production pack hardware. We are optimizing the biomanufacturing platform for in situ production of yogurt and kefir on the ISS. BN-2 also will test engineered microorganisms, including a probiotic strain, to produce carotenoids or follistatin. To improve the feasibility of this technology for mission use, we transitioned from a hard-shell pack to a second-generation fluorinated ethylene propylene (FEP) bag. The single-use FEP bioreactor facilitates growth of target organisms in a contaminant free environment, while reducing mass and volume compared to the BN-1 hardware. These bioreactors will be employed for production of additional space-relevant products in the BN-2 project. On-orbit testing of FEP production packs will enable optimization of the platform for eventual flight use by the crew.

Natalie Ball↗

NASA NTRS2012

Nuclear Thermal Rocket (Ntr) Propulsion: A Proven Game-Changing Technology for Future Human Exploration Missions

The NTR represents the next evolutionary step in high performance rocket propulsion. It generates high thrust and has a specific impulse (Isp) of approx.900 seconds (s) or more V twice that of today s best chemical rockets. The technology is also proven. During the previous Rover and NERVA (Nuclear Engine for Rocket Vehicle Applications) nuclear rocket programs, 20 rocket reactors were designed, built and ground tested. These tests demonstrated: (1) a wide range of thrust; (2) high temperature carbide-based nuclear fuel; (3) sustained engine operation; (4) accumulated lifetime; and (5) restart capability V all the requirements needed for a human mission to Mars. Ceramic metal cermet fuel was also pursued, as a backup option. The NTR also has significant growth and evolution potential. Configured as a bimodal system, it can generate electrical power for the spacecraft. Adding an oxygen afterburner nozzle introduces a variable thrust and Isp capability and allows bipropellant operation. In NASA s recent Mars Design Reference Architecture (DRA) 5.0 study, the NTR was selected as the preferred propulsion option because of its proven technology, higher performance, lower launch mass, simple assembly and mission operations. In contrast to other advanced propulsion options, NTP requires no large technology scale-ups. In fact, the smallest engine tested during the Rover program V the 25,000 lbf (25 klbf) Pewee engine is sufficient for human Mars missions when used in a clustered engine arrangement. The Copernicus crewed spacecraft design developed in DRA 5.0 has significant capability and a human exploration strategy is outlined here that uses Copernicus and its key components for precursor near Earth asteroid (NEA) and Mars orbital missions prior to a Mars landing mission. Initially, the basic Copernicus vehicle can enable reusable 1-year round trip human missions to candidate NEAs like 1991 JW and Apophis in the late 2020 s to check out vehicle systems. Afterwards, the Copernicus spacecraft and its 2 key components, now configured as an Earth Return Vehicle / propellant tanker, would be used for a short round trip (approx.18 - 20 months)/short orbital stay (60 days) Mars / Phobos survey mission in 2033 using a split mission approach. The paper also discusses NASA s current Foundational Technology Development activities and its pre-decisional plans for future system-level Technology Demonstrations that include ground testing a small (approx.7.5 klbf) scalable NTR before the decade is out with a flight test shortly thereafter.

Borowski, Stanley K.↗

NASA NTRS2005

Incremental Scheduling Engines: Cost Savings through Automation

As humankind embarks on longer space missions farther from home, the requirements and environments for scheduling the activities performed on these missions are changing. As we begin to prepare for these missions it is appropriate to evaluate the merits and applicability of the different types of scheduling engines. Scheduling engines temporally arrange tasks onto a timeline so that all constraints and ob.jectives are met and resources are not over-booked. Scheduling engines used to schedule space missions fall into three general categories: batch, mixed-initiative, and incremental. This paper, presents an assessment of the engine types, a discussion of the impact of human exploration of the moon and Mars on planning and scheduling, and the applicability of the different types of scheduling engines. This paper will pursue the hypothesis that incremental scheduling engines may have a place in the new environment; they have the potential to reduce cost, to improve the satisfaction of those who execute or benefit from a particular timeline (the customers), and to allow astronauts to plan their own tasks and those of their companion robots.

Jaap, John↗

NASA NTRSDate not supplied

Feasibility Study of a Multi-Tilt-Rotor Aircraft as the Artemis Lunar Training Vehicle

The Lunar Landing Research Vehicles (LLRVs) and the Lunar Landing Training Vehicles (LLTVs) provided astronauts of the Apollo program with essential experience and confidence required to complete the missions, and contributed to six successful manned landings on the moon. The primary challenge in terrestrial training was being able to replicate the ratio of tilt angle to linear acceleration that a pilot would experience in lunar gravity. Presently, as the Artemis program seeks to return humans to the Moon by 2025, engineers are evaluating suitable platforms to serve as an In-Flight Trainer (IFT) or Artemis Lunar Training Vehicle (ALTV) for astronauts training in the task of manual landing. The program is investigating the viability of current technology in the field of electric vertical takeoff and landing (eVTOL) vehicles and is evaluating using a multi-tilt-rotor aircraft platform as a candidate for a preliminary ALTV. The tilt-rotor capability enables the vehicle attitude to be decoupled from its flight path, which is a crucial requirement in realistically simulating lunar gravity on Earth. Other key considerations include compensating for a lack of aerodynamic forces while flying through the atmosphere of Earth, as well as the ability to simulate the dynamics of multiple different lander designs for the Human Landing System (HLS) program. This paper details the feasibility study and presents a preliminary flight control architecture for an IFT based on a notional multi-tilt-rotor platform. The model-following control law, based on nonlinear dynamic inversion (NDI), removes the need for gain scheduling. The inner-loop dynamic control allocation strategy consists of a static portion that is optimized offline for trim while compensating for the difference in gravity and a dynamic portion that is computed in real time. The reference model consists of the full closed-loop dynamics of a generic HLS design. The modularity of the flight control architecture enables evaluation of multiple HLS concepts with minimal modifications to the control law. Simulation results of the multi-tilt-rotor configuration following the final portion of the Apollo 11 descent trajectory are shown.

Jing Pei↗

NASA NTRSDate not supplied

Feasibility Study of a Multi Tilt-rotor Aircraft as the Artemis Lunar Training Vehicle

The Lunar Landing Research Vehicles (LLRVs) and the Lunar Landing Training Vehicles (LLTVs) provided astronaut candidates for the Apollo program with essential experience and confidence required to complete the missions, and contributed to six successful manned landings on the moon. The primary challenge in terrestrial training was being able to replicate the ratio of bank angle to linear acceleration that a pilot would experience in lunar gravity. Presently, as the Artemis program seeks to return humans to the Moon by 2025, engineers are evaluating suitable platforms to serve as an In-Flight Trainer (IFT) or Artemis Lunar Training Vehicle (ALTV) for astronauts training in the task of manual landing. The program is investigating the viability of current technology in the field of electric vertical takeoff and landing (eVTOL) vehicles and is evaluating using a multi tilt-rotor aircraft platform as a candidate platform for a preliminary ALTV. The tilt-rotor capability enables the vehicle attitude to be decoupled from its flight path, which is a crucial requirement in realistically simulating lunar gravity on Earth. Other key considerations include compensating for a lack of aerodynamic forces while flying through the atmosphere of Earth, as well as the ability to simulate the dynamics of multiple different lander designs for the Human Landing System (HLS) program. This paper details the feasibility study and presents a preliminary flight control architecture for an IFT based on a notional multi tilt-rotor platform. The modeling-following control law, based on nonlinear dynamic inversion (NDI), removes the need for gain scheduling because the vehicle operates across a wide range of flight conditions. The inner-loop dynamic control allocation strategy consists of a static portion that is optimized offline for trim while compensating for the difference in gravity and a dynamic portion that is computed in real time. The reference model consists of the full closed-loop dynamics of a generic HLS design. The modularity of the flight control architecture enables evaluation of multiple HLS concepts with minimal modifications to the control law. Simulation results of the multi tilt-rotor configuration following the final portion of the Apollo 11 descent trajectory are shown.

Jing Pei↗