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At least 739 records · Page 41

Three-dimensional tissue assemblies: novel models for the study of Salmonella enterica serovar Typhimurium pathogenesis

The lack of readily available experimental systems has limited knowledge pertaining to the development of Salmonella-induced gastroenteritis and diarrheal disease in humans. We used a novel low-shear stress cell culture system developed at the National Aeronautics and Space Administration in conjunction with cultivation of three-dimensional (3-D) aggregates of human intestinal tissue to study the infectivity of Salmonella enterica serovar Typhimurium for human intestinal epithelium. Immunohistochemical characterization and microscopic analysis of 3-D aggregates of the human intestinal epithelial cell line Int-407 revealed that the 3-D cells more accurately modeled human in vivo differentiated tissues than did conventional monolayer cultures of the same cells. Results from infectivity studies showed that Salmonella established infection of the 3-D cells in a much different manner than that observed for monolayers. Following the same time course of infection with Salmonella, 3-D Int-407 cells displayed minimal loss of structural integrity compared to that of Int-407 monolayers. Furthermore, Salmonella exhibited significantly lower abilities to adhere to, invade, and induce apoptosis of 3-D Int-407 cells than it did for infected Int-407 monolayers. Analysis of cytokine expression profiles of 3-D Int-407 cells and monolayers following infection with Salmonella revealed significant differences in expression of interleukin 1alpha (IL-1alpha), IL-1beta, IL-6, IL-1Ra, and tumor necrosis factor alpha mRNAs between the two cultures. In addition, uninfected 3-D Int-407 cells constitutively expressed higher levels of transforming growth factor beta1 mRNA and prostaglandin E2 than did uninfected Int-407 monolayers. By more accurately modeling many aspects of human in vivo tissues, the 3-D intestinal cell model generated in this study offers a novel approach for studying microbial infectivity from the perspective of the host-pathogen interaction.

NASA Discipline Environmental Health↗

Optical Performance Modeling of FUSE Telescope Mirror

We describe the Metrology Data Processor (METDAT), the Optical Surface Analysis Code (OSAC), and their application to the image evaluation of the Far Ultraviolet Spectroscopic Explorer (FUSE) mirrors. The FUSE instrument - designed and developed by the Johns Hopkins University and launched in June 1999 is an astrophysics satellite which provides high resolution spectra (lambda/Delta(lambda) = 20,000 - 25,000) in the wavelength region from 90.5 to 118.7 nm The FUSE instrument is comprised of four co-aligned, normal incidence, off-axis parabolic mirrors, four Rowland circle spectrograph channels with holographic gratings, and delay line microchannel plate detectors. The OSAC code provides a comprehensive analysis of optical system performance, including the effects of optical surface misalignments, low spatial frequency deformations described by discrete polynomial terms, mid- and high-spatial frequency deformations (surface roughness), and diffraction due to the finite size of the aperture. Both normal incidence (traditionally infrared, visible, and near ultraviolet mirror systems) and grazing incidence (x-ray mirror systems) systems can be analyzed. The code also properly accounts for reflectance losses on the mirror surfaces. Low frequency surface errors are described in OSAC by using Zernike polynomials for normal incidence mirrors and Legendre-Fourier polynomials for grazing incidence mirrors. The scatter analysis of the mirror is based on scalar scatter theory. The program accepts simple autocovariance (ACV) function models or power spectral density (PSD) models derived from mirror surface metrology data as input to the scatter calculation. The end product of the program is a user-defined pixel array containing the system Point Spread Function (PSF). The METDAT routine is used in conjunction with the OSAC program. This code reads in laboratory metrology data in a normalized format. The code then fits the data using Zernike polynomials for normal incidence systems or Legendre-Fourier polynomials for grazing incidence systems. It removes low order terms from the metrology data, calculates statistical ACV or PSD functions, and fits these data to OSAC models for the scatter analysis. In this paper we briefly describe the laboratory image testing of FUSE spare mirror performed in the near and vacuum ultraviolet at John Hopkins University and OSAC modeling of the test setup performed at NASA/GSFC. The test setup is a double-pass configuration consisting of a Hg discharge source, the FUSE off-axis parabolic mirror under test, an autocollimating flat mirror, and a tomographic imaging detector. Two additional, small fold flats are used in the optical train to accommodate the light source and the detector. The modeling is based on Zernike fitting and PSD analysis of surface metrology data measured by both the mirror vendor (Tinsley) and JHU. The results of our models agree well with the laboratory imaging data, thus validating our theoretical model. Finally, we predict the imaging performance of FUSE mirrors in their flight configuration at far-ultraviolet wavelengths.

Saha, Timo T.↗

Along-Orbit Analysis of GRACE Follow-On Inter-Satellite Laser Ranging Measurements for Sub-Monthly Surface Mass Variations

We examined the sensitivity of GRACE Follow-On (GRACE-FO) laser ranging interferometer (LRI) measurements to sub-monthly time-variable gravity (TVG) signals caused by transient, high-frequency mass changes in the Earth system. GRACE-FO LRI provides complementary inter-satellite ranging measurements with higher precision over a wider range of frequencies than the baseline K-band microwave ranging system. The common approach for studying mass variation relies on the inverted TVG or mascon solutions over a period of, for example, one month or 10 days which are adversely affected by temporal aliasing and/or smoothing. In this article, we present the alternative along-orbit analysis methodology in terms of line-of-sight gravity difference (LGD) to fully exploit the higher precision LRI measurements for examination of sub-monthly mass changes. The discrepancy between “instantaneous” LGD LRI observations and monthly mean LGD (from Level-2 data) at satellite altitude indicates the sub-monthly gravitational variability not captured by monthly-mean solutions. In conjunction with the satellite ocean altimetry observations, high frequency non-tidal atmosphere and ocean models, and hydrology models, we show that the LGD LRI observations detect the high-frequency oceanic mass variability in the Argentine Basin and the Gulf of Carpentaria, and sub-monthly variations in surface (river) water in the Amazon Basin. We demonstrate the benefits gained from repeat ground track analysis of GRACE-FO LRI data in the case of the Amazon surface water flow. The along-orbit analysis methodology based on LGD LRI time series presented here is especially suitable for quantifying temporal and spatial evolution of extreme, rapidly changing mass variations.

Khosro Ghobadi-Far↗

Numerical analysis of flow about a total temperature sensor

The unsteady flowfield about an airfoil-shaped inlet temperature sensor has been investigated using the thin-layer and full Navier-Stokes equations. A finite-volume formulation of the governing equations was used in conjunction with a Runge-Kutta time stepping scheme to analyze the flow about the sensor. Flow characteristics for this configuration were established at Mach numbers of 0.5 and 0.8 for different Reynolds numbers. The results were obtained for configurations of increasing complexity; important physical phenomena such as shock formation, boundary-layer separation, and unsteady wake formation were noted. Based on the computational results, recommendations for further study and refinement of the inlet temperature sensor were made.

Von Lavante, Ernst↗

MGS/TES-Odyssey/THEMIS-IR Analysis of Localized Low Albedo Regions in Valles Marineris

We are conducting a systematic analysis of small (approximately 10's of km), localized regions in Valles Marineris that display significant albedo differences relative to their surroundings. This analysis is based on a finding that the locations of the hematite deposits identified by [1] in the interior layered deposits of Valles Marineris typically coincide with regions having a low MGS/TES visible bolometric albedo [1,2]. Until recently, it was difficult to identify the morphology or geologic context of the regions containing the hematite deposits. However, with the recent advent of high-resolution (1/128 /pixel) MOLA grided topography and Mars Odyssey s THEMIS-IR instrument, it has been possible to better understand the morphologic context of TES observations. This analysis combines the use of PDS-released data from the MGS/TES visible bolometer and infrared spectrometer, the Odyssey/THEMIS Infrared imager, and MOLA grided topography. First, the TES infrared bolometer is used to identify regions of interesting albedo variability, and is overlaid on Viking controlled photomosaics for context. THEMIS-IR data, in conjunction with MOLA topography, is then used to: 1) identify the context and morphology of the area; and 2) identify spectrally unique regions at the km scale. In preparation for the latter, all the THEMIS planes are coregistered using an autocorrelation routine, the data are converted to brightness temperature and then each plane is normalized to the brightness temperature of the third plane (1261 cm-1). We then perform a 3-band search for color variations and a Principle Components Analysis (PCA) of the 8 unique bands in the THEMIS-IR dataset. Any variability is then investigated using both THEMIS-IR and TES spectra of the same regions. In both cases, the spectra are ratioed to near-simultaneously acquired spectra of adjacent or "average" regions that do not show this albedo variation, therefore allowing us to identify spectral variability unique to the area of interest. This procedure also allows us to account for calibration problems in THEMIS-IR data, and for any atmospheric effects in both the THEMIS-IR and the TES data.

E Z Noe Dobrea↗

Elastohydrodynamic lubrication of smooth surfaces

Fully flooded, elastohydrodynamically lubricated contacts are considered. Elastohydrodynamic lubrication (EHL) analysis requires the simultaneous solution of the elastically, viscosity, density, and Reynolds equations. The most important practical aspect of elastohydrodynamic lubrication theory is the determination of the minimum film thickness within the conjunction. The maintenance of a fluid film of adequate magnitude is an essential feature of the correct operation of lubricated machine elements. The results show the influence of contact geometry on minimum film thickness as expressed by the ellipticity parameter and the dimensionless speed, load, and materials parameters. Film thickness equations are developed for materials of high elastic modulus, such as metal, and for materials of low elastic modulus, such as rubber.

Hamrock, B. J.↗

Eigenvalue computation of large symmetric tridiagonal matrices on concurrent processors

Symmetric tridiagonal eigenvalue problems may arise indirectly in structural dynamic analysis. An algorithm for eigenvalue computation of large symmetric tridiagonal matrices on concurrent processors to meet the challenge of the new emerging computer hardware technology is presented. A standard bisection method in conjunction with Sylvester's Theorem is chosen to be converted into a parallel N-section algorithm. This parallel algorithm takes advantage of the multi-processor environment by carrying out N (number of processors) triangular factorizations of chosen shifted matrices in all processors concurrently and by minimizing communication between processors. The algorithm is designed for local-memory concurrent processors, i.e. message passing type processors. The efficiency and speed-up are given in terms of problem and machine parameters. The algorithm is very efficient when both the number of processors and the number of eigenvalues to be extracted are much smaller than the order of the tridiagonal matrix.

Chang, H. Y.↗

A 3-dimensional finite-difference method for calculating the dynamic coefficients of seals

A method to calculate the dynamic coefficients of seals with arbitrary geometry is presented. The Navier-Stokes equations are used in conjunction with the k-e turbulence model to describe the turbulent flow. These equations are solved by a full 3-dimensional finite-difference procedure instead of the normally used perturbation analysis. The time dependence of the equations is introduced by working with a coordinate system rotating with the precession frequency of the shaft. The results of this theory are compared with coefficients calculated by a perturbation analysis and with experimental results.

Dietzen, F. J.↗

The Space and Earth Science Data Compression Workshop

This document is the proceedings from a Space and Earth Science Data Compression Workshop, which was held on March 27, 1992, at the Snowbird Conference Center in Snowbird, Utah. This workshop was held in conjunction with the 1992 Data Compression Conference (DCC '92), which was held at the same location, March 24-26, 1992. The workshop explored opportunities for data compression to enhance the collection and analysis of space and Earth science data. The workshop consisted of eleven papers presented in four sessions. These papers describe research that is integrated into, or has the potential of being integrated into, a particular space and/or Earth science data information system. Presenters were encouraged to take into account the scientists's data requirements, and the constraints imposed by the data collection, transmission, distribution, and archival system.

Tilton, James C.↗

Design of a wing shape for study of hypersonic crossflow transition in flight

Computational fluid dynamics methods were used in the design of a wing shape for study of hypersonic crossflow transition in flight. The flight experiment is to be performed on the delta wing of the first stage of a Pegasus launch vehicle as a piggy-back experiment to support boundary-layer stability code development and validation. The design goal is to obtain crossflow-induced transition at 20-40 percent of the chord for a flight Mach number of approximately six. The present paper describes the design and analysis process utilized to obtain desired glove shape. A variety of schemes were used in the design, ranging from simple empirical crossflow correlations to three-dimensional Navier-Stokes codes in conjunction with linear stability/N-factor computations. The sensitivity to various parameters, such as trajectory variations, allowable wing thickness, leading-edge radius and surface temperature, is also discussed.

Godil, A.↗

Extra-Zodiacal-Cloud Astronomy via Solar Electric Propulsion

Solar electric propulsion (SEP) is often considered as primary propulsion for robotic planetary missions, providing the opportunity to deliver more payload mass to difficult, high-delta-velocity destinations. However, SEP application to astrophysics has not been well studied. This research identifies and assesses a new application of SEP as primary propulsion for low-cost high-performance robotic astrophysics missions. The performance of an optical/infrared space observatory in Earth orbit or at the Sun-Earth L2 point (SEL2) is limited by background emission from the Zodiacal dust cloud that has a disk morphology along the ecliptic plane. By delivering an observatory to a inclined heliocentric orbit, most of this background emission can be avoided, resulting in a very substantial increase in science performance. This advantage enabled by SEP allows a small-aperture telescope to rival the performance of much larger telescopes located at SEL2. In this paper, we describe a novel mission architecture in which SEP technology is used to enable unprecedented telescope sensitivity performance per unit collecting area. This extra-zodiacal mission architecture will enable a new class of high-performance, short-development time, Explorer missions whose sensitivity and survey speed can rival flagship-class SEL2 facilities, thus providing new programmatic flexibility for NASA's astronomy mission portfolio. A mission concept study was conducted to evaluate this application of SEP. Trajectory analyses determined that a 700 kg-class science payload could be delivered in just over 2 years to a 2 AU mission orbit inclined 15 to the ecliptic using a 13 kW-class NASA's Evolutionary Xenon Thruster (NEXT) SEP system. A mission architecture trade resulted in a SEP stage architecture, in which the science spacecraft separates from the stage after delivery to the mission orbit. The SEP stage and science spacecraft concepts were defined in collaborative engineering environment studies. The SEP stage architecture approach offers benefits beyond a single astrophysics mission. A variety of low-cost astrophysics missions could employ a standard SEP stage to achieve substantial science benefit. This paper describes the results of this study in detail, including trajectory analysis, spacecraft concept definition, description of telescope/instrument benefits, and application of the resulting SEP stage to other missions. In addition, the benefits of cooperative development and use of the SEP stage, in conjunction with a SEP flight demonstration mission currently in definition at NASA, are considered.

Benson, Scott W.↗

Cultivating an Emergent Earth Observation Analytics Ecosystem in the Cloud

A diverse set of data analytics systems for Earth Observations are sprouting up in the Earth Science community, with a wealth of processing algorithms and analysis methods. There is a similar wealth of data resources available via myriad data providers and clearinghouses, including large institutional systems like the Earth Observing System Data and Information System, Comprehensive Large Scale Array-data Stewardship System, and Federated Earth Observation Missions gateway. With Earth system science driving a need to work with more datasets together, and the community developing more analysis tools (some of them dataset-specific), how can we develop analysis workflows that incorporate far-flung datasets and leverage analysis resources from multiple organizations? Cloud computing points the way toward a solution in two different respects. Firstly, the access to and abstraction of virtually unlimited storage and computing power provides an environment that enables more straightforward means of pulling datasets and analysis resources together. Just as importantly, however, cloud computing serves as an example of an "ecosystem" of interoperating services, since the essence of cloud computing is the presentation of all resources as a service, from hardware to infrastructure to platform to software. This enables the combination of off-the-shelf, diverse services to construct entire systems that emerge out of an equally diverse community of architects and developers. This approach can be similarly applied to the data and analysis resources in the Earth Observation community. By exposing these resources via well understood services, and consuming resources in the same way, different organizations can construct bespoke analysis workflows and systems for their own purposes. The key leap the community needs to make is to develop analysis systems in components that interact with other components via services. The result would be a rich ecosystem of analytics components that can be combined to analyze datasets at scale and in conjunction with other datasets from other sources.

chaos↗

Simulation and Flight Test Data Collection to Support Phase 1 Detect-and-Avoid MOPS

The UAS Integration into the NAS (UASNAS) project is studying the minimum operational performance standards for unmanned aerial system (UAS) detect-and-avoid (DAA) systems in order to operate in the National Airspace System (NAS). The project is executing a series of fast-time simulation, human-in-the-loop experiments, and flight tests in support of this effort. Armstrong Flight Research Center is hosting a VIP Day in conjunction with the UAS-NAS Project's Flight Test 4 activity, and the Ames Project Engineer (or designate) will be presenting a briefing. This briefing will review the data collection and analysis activities for Flight Test 4 within the Separation assurance-Sense-and-avoid Interoperability (SSI) team.

data analysis↗

Environmental Barrier Coating Fracture, Fatigue and High-Heat-Flux Durability Modeling and Stochastic Progressive Damage Simulation

Advanced environmental barrier coatings will play an increasingly important role in future gas turbine engines because of their ability to protect emerging light-weight SiC/SiC ceramic matrix composite (CMC) engine components, further raising engine operating temperatures and performance. Because the environmental barrier coating systems are critical to the performance, reliability and durability of these hot-section ceramic engine components, a prime-reliant coating system along with established life design methodology are required for the hot-section ceramic component insertion into engine service. In this paper, we have first summarized some observations of high temperature, high-heat-flux environmental degradation and failure mechanisms of environmental barrier coating systems in laboratory simulated engine environment tests. In particular, the coating surface cracking morphologies and associated subsequent delamination mechanisms under the engine level high-heat-flux, combustion steam, and mechanical creep and fatigue loading conditions will be discussed. The EBC compostion and archtechture improvements based on advanced high heat flux environmental testing, and the modeling advances based on the integrated Finite Element Analysis Micromechanics Analysis Code/Ceramics Analysis and Reliability Evaluation of Structures (FEAMAC/CARES) program will also be highlighted. The stochastic progressive damage simulation successfully predicts mud flat damage pattern in EBCs on coated 3-D specimens, and a 2-D model of through-the-thickness cross-section. A 2-parameter Weibull distribution was assumed in characterizing the coating layer stochastic strength response and the formation of damage was therefore modeled. The damage initiation and coalescence into progressively smaller mudflat crack cells was demonstrated. A coating life prediction framework may be realized by examining the surface crack initiation and delamination propagation in conjunction with environmental degradation under high-heat-flux and environment load test conditions.

Mechanical creep and fatigue loading condition↗

New evidence for solar cycle variations at great distances

Recent studies of solar planetary relationships are directed toward exploring how far out from the sun one could observe solar cycle variations. A positive solar Jovian relationship is suggested from a Chree superposed epoch study of the intensity of the great red spot of Jupiter over a period of about six solar cycles. The characteristic double maxima observed in the solar cycle variation is common to other observations of solar events in the photosphere, chromosphere, and corona; radio and corpuscular emissions from the sun; cosmic ray intensity and geomagnetic activity. The same method of analysis adopted for the study of luminosity changes of the planets Jupiter, Saturn, Uranus, and Neptune indicates that the fluctuations of luminosity follow the single maximum solar cycle represented by sunspot numbers. In conjunction with changes of upper atmospheric density and temperature, it is suggested that the extreme ultraviolet (EUV) emission from the sun may be connected with luminosity changes. A method of distinguishing between phenomena related to solar wind and those related to solar EUV is presented.

Venkatesan, D.↗

Utilizing remote sensing data for land use decisions for Indian lands in South Dakota.

Evaluation of photographic and thermal infrared imagery acquired at an altitude of 11,500 ft above ground level (in southwestern South Dakota) on Oct. 15, 1970, June 30, 1971, and Aug. 2, 1971, for use in mapping range sites and soils. A density slicing system was used to enhance film optical density differences associated with range sites and soils. The range site boundaries in native rangeland areas were found to be delineated best by a density slicing analysis of color infrared film obtained in August 1971. The range site maps produced by density slicing were superior to existing range inventory maps. The density slicing analysis of color infrared film from the August 1971 flight was best for locating soil boundaries in rangeland areas. To adequately map soils in rangeland areas, data based on density slicing used in conjunction with slope gradient information derived from stereoscopic analyses provided more detailed and accurate information than is presently available from range site and soil maps of the area.

Frazee, C. J.↗

Application of a system modification technique to dynamic tuning of a spinning rotor blade

An important consideration in the development of modern helicopters is the vibratory response of the main rotor blade. One way to minimize vibration levels is to ensure that natural frequencies of the spinning main rotor blade are well removed from integer multiples of the rotor speed. A technique for dynamically tuning a finite-element model of a rotor blade to accomplish that end is demonstrated. A brief overview is given of the general purpose finite element system known as Engineering Analysis Language (EAL) which was used in this work. A description of the EAL System Modification (SM) processor is then given along with an explanation of special algorithms developed to be used in conjunction with SM. Finally, this technique is demonstrated by dynamically tuning a model of an advanced composite rotor blade.

Spain, C. V.↗

Adaptive p-version based finite element formulations for thermal modeling/analysis of structural configurations

Adaptive p-version based hierarchical finite element formulations in conjunction with a posteriori error estimation concepts are described with emphasis on applicability for thermal modeling/analysis of structural configurations. The basic concepts and formulations of hierarchical p-versiion finite element for thermal analysis are first described. A posteriori error estimation features are utililzed to steer the process of adaptive refinement. Several configurations comprised of one-dimensional structures are evaluated to validate the applicability of the proposed formulations and to demonstrate the potential of the p-version adaptive formulations for thermal modeling/analysis. The methodology offers potential and promises to be an attractive alternative to conventional finite element thermal modeling/analysis approaches.

Tamma, Kumar K.↗