Search NASA⌕ Search

SEARCH · Search NASA

Results for “soil structure”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 739 records · Page 41

The Mars Pathfinder Mission

Mars Pathfinder, one of the first Discovery-class missions (quick, low-cost projects with focused science objectives), will land a single spacecraft with a microrover and several instruments on the surface of Mars in 1997. Pathfinder will be the first mission to use a rover, carrying a chemical analysis instrument, to characterize the rocks and soils in a landing area over hundreds of square meters on Mars, which will provide a calibration point or "ground truth" for orbital remote sensing observations. In addition to the rover, which also performs a number of technology experiments, Pathfinder carries three science instruments: a stereoscopic imager with spectral filters on an extendable mast, an alpha proton X ray spectrometer, and an atmospheric structure instrument/meteorology package. The instruments, the rover technology experiments, and the telemetry system will allow investigations of the surface morphology and geology at submeter to a hundred meters scale, the petrology and geochemistry of rocks and soils, the magnetic properties of dust, soil mechanics and properties, a variety of atmospheric investigations, and the rotational and orbital dynamics of Mars. Landing downstream from the mouth of a giant catastrophic outflow channel, Ares Vallis at 19.5 deg N, 32.8 deg W, offers the potential of identifying and analyzing a wide variety of crustal materials, from the ancient heavily cratered terrain, intermediate-aged ridged plains, and reworked channel deposits, thus allowing first-order scientific investigations of the early differentiation and evolution of the crust, the development of weathering products, and tile early environments and conditions on Mars.

Golombek, Matthew P.↗

Forest Structural Complexity and Biomass Predict First-Year Carbon Cycling Responses to Disturbance

The pre-disturbance vegetation characteristics that predict carbon (C) cycling responses to disturbance are not well known. To address this gap, we initiated the Forest Resilience Threshold Experiment, a manipulative study in which more than 3600 trees were stem girdled to achieve replicated factorial combinations of four levels (control, 45, 65, and 85% gross defoliation) of disturbance severity and two disturbance types (targeting upper or lower canopy strata). Applying a standardized stability framework in which initial C cycling resistance to disturbance was calculated as the first-year natural log response ratio of disturbance and control treatments, we investigated to what extent pre-disturbance levels of species diversity, aboveground woody biomass, leaf area index, and canopy rugosity—a measure of structural complexity—predict the initial responses of subcanopy light-saturated leaf CO 2 assimilation (A sat ), aboveground wood NPP (ANPP w ), and soil respiration (R s ) to phloem-disrupting disturbance. In the year following stem girdling, we found that above-ground C cycling processes, A sat and ANPP w , were highly resistant to increases in disturbance severity, while Rs resistance declined as severity increased. Disturbance type had no effect on first-year resistance. Pre-disturbance aboveground woody biomass, and canopy rugosity were positive predictors of ANPP w resistance and, conversely, negatively related to R s resistance. Subcanopy A sat resistance was not related to pre-disturbance vegetation characteristics. Stability of C uptake processes along with R s declines suggest the net C sink was sustained in the initial months following disturbance. Therefore, we conclude that biomass and complexity are significant, but not universal, predictors of initial C cycling resistance to disturbance. Moreover, our findings highlight the utility of standardized stability measures when comparing functional responses to disturbance.

54 ENVIRONMENTAL SCIENCES↗

Long-Term Cellulose Enrichment Selects for Highly Cellulolytic Consortia and Competition for Public Goods

Microbial communities are a key driver of the carbon cycle through the breakdown of complex polysaccharides in diverse environments including soil, marine systems, and the mammalian gut. However, due to the complexity of these communities, the species-species interactions that impact community structure and ultimately shape the rate of decomposition are difficult to define.

microbial interactions↗

AmeriFlux FLUXNET-1F US-xTL NEON Toolik (TOOL)

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-xTL NEON Toolik (TOOL). This is the FLUXNET version of the carbon flux data for the site US-xTL NEON Toolik (TOOL) produced by applying the standard ONEFlux (1F) software. Site Description - The Toolik field site is located in a remote wilderness area at the Toolik Field Station which is on land managed by the Bureau of Land Management. The area is underlain by continuous permafrost, which exerts a major influence on hydrology and the distribution, structure, and function of terrestrial and aquatic ecosystems. Because of its location between the Brooks Range and the coastal plain, the vegetation and soils at TOOL are representative of much of the Alaskan foothills tundra. This terrestrial field site is collocated with NEON’s Toolik Lake aquatic site. The closest city, Fairbanks, Alaska is 400 miles away. The Dalton Highway (also known as the Haul Road or Pipeline Road), which traverses the middle of the state from Fairbanks to Prudhoe Bay, provides access to the area.

Network), NEON (National Ecological Observatory↗

Machine intelligence and robotics: Report of the NASA study group. Executive summary

A brief overview of applications of machine intelligence and robotics in the space program is given. These space exploration robots, global service robots to collect data for public service use on soil conditions, sea states, global crop conditions, weather, geology, disasters, etc., from Earth orbit, space industrialization and processing technologies, and construction of large structures in space. Program options for research, advanced development, and implementation of machine intelligence and robot technology for use in program planning are discussed. A vigorous and long-range program to incorporate and keep pace with state of the art developments in computer technology, both in spaceborne and ground-based computer systems is recommended.

Source record↗

Flat-plate solar array project. Volume 7: Module encapsulation

The objective of the Encapsulation Task was to develop, demonstrate, and qualify photovoltaic (PV) module encapsulation systems that would provide 20 year (later decreased to 30 year) life expectancies in terrestrial environments, and which would be compatible with the cost and performance goals of the Flat-Plate Solar Array (FSA) Project. The scope of the Encapsulation Task included the identification, development, and evaluation of material systems and configurations required to support and protect the optically and electrically active solar cell circuit components in the PV module operating environment. Encapsulation material technologies summarized include the development of low cost ultraviolet protection techniques, stable low cost pottants, soiling resistant coatings, electrical isolation criteria, processes for optimum interface bonding, and analytical and experimental tools for evaluating the long term durability and structural adequacy of encapsulated modules. Field testing, accelerated stress testing, and design studies have demonstrated that encapsulation materials, processes, and configurations are available that meet the FSA cost and performance goals.

Cuddihy, E.↗

Effects of fire on composition, biomass, and nutrients in oak scrub vegetation on John F. Kennedy Space Center, Florida

Four stands of oak scrub two, four, eight, and 25 years since fire were sampled with permanent 15 m line transects. Percent cover by species was determined. Plant samples were analyzed for a variety of substances. Transects were resurveyed in 1985 for vegetation parameters. Nutrient pools in biomass were calculated from biomass data and tissue nutrient concentrations. Soil nutrient pools were calculated from nutrient concentrations and bulk density. Species distribution and soil chemical properties were found to be closely related to water table depth. The following fire-related conclusions are reached: (1) major structural changes occur in scrub after fire in that shrub height is reduced and requires four to six years to exceed 1 m; (2) reduction in shrub height affects the suitability of scrub for the Florida scrub jay (3) live biomass increases with time since fire; (4) nutrient concentrations in live biomass do not change with time since fire; (5) species composition and richness are little changed after fire; and (6) imposition of a continued regime of burning on a three-year cycle may have adverse impacts not indicated by the recovery of scrub from a single fire.

Schmalzer, Paul A.↗

Design criteria for an underground lunar mine

Underground excavation and construction techniques have been well developed terrestrially and provide an attractive option for lunar mining and habitat construction. The lunar mine, processing facilities and habitats could be located beneath the lunar surface in basaltic rock that would protect the crew and equipment from the hazardous surface environment. A terrestrial-like atmosphere would be created within the underground structures allowing more conventional technologies to be utilized. In addition, the basalt would likely contain higher quality mineral deposits than the regolith (lunar soil) since the minerals in the regolith have been degraded by meteorite bombardment. The conditions that would affect the design of an underground lunar mine are described and a lunar rock mass rated to assess its quality using terrestrial rock mass classification systems. Design criteria are established and a construction scenario proposed. Parameters having the greatest effect on stability are identified based on distinct element computer modeling and terrestrial experience.

Siekmeier, John A.↗

In-Situ XRD/XRF to Support Life Detection on Mars

X-ray diffraction / X-ray fluorescence (XRD/XRF) analysis provides the most comprehensive mineralogical / compositional characterization of rocks and soils of any flight-capable technique. XRD data provide quantitative mineralogy (including abundance of X-ray amorphous materials) and crystal chemistry (structure, elemental composition and valence state), and XRF data provide complimentary major, minor, and some trace element abundances. Both types of data are important in evaluating habitability (environment of formation) and biosignature preservation/degradation (post-depositional diagenetic change). Whether or not a relict biosignature is detected, the mineral assemblage and its geochemistry can be used to determine the habitability of an ancient environment (e.g., salinity, pH, temperature), and to identify potential sources of energy for life (e.g., elements in different redox states). In this respect, a null result (a habitable environment lacking evidence of life) can play an important role in constraining the parameters of the search. Conversely, diagenetic alteration (taphonomic change) resulting from post-depositional variations in temperature, pressure or fluid chemistry can preserve evidence of biogenicity, erase such evidence completely or indeed can provide for post-depositional habitable conditions in the subsurface. XRD / XRF data are critical to these determinations. The CheMin instrument on the Mars Science Laboratory (MSL) Curiosity rover is the first XRD instrument flown in space. CheMin operates in transmission geometry with a Co X-ray source to minimize fluorescence from iron. Diffracted photons are collected with an energy-sensitive charge-coupled device (CCD). The position of the diffracted photons provides structural information for minerals, whereas the energy of sample-generated X-ray fluorescence photons provides elemental information, though these XRF data are qualitative. Mineralogical data from the CheMin XRD identified the three circumstances above: habitable depositional environments (e.g., Yellowknife Bay), habitable subsurface/diagenetic environments (e.g., throughout the Murray formation), and diagenetic conditions that may destroy evidence of habitability (e.g., oxidative and acidic environments at Vera Rubin ridge). Technological advances in X-ray technology and lessons learned from the operation of CheMin on Mars have resulted in a next-generation XRD/XRF, called CheMinX. Replacement of CheMin’s CCD with an array of hybrid pixel detectors and improvements in focusing optics dramatically decrease analysis time (15 minutes vs. 22 hours for MSL-CheMin) and result in a better angular resolution (0.18 vs. 0.30 °2θ for MSL-CheMin). This increased resolution improves mineral detection, including discrimination between types of pyroxenes, which is not possible with MSL-CheMin data. The hybrid pixel detectors do not require cooling like the MSL-CheMin CCD, therefore reducing the power needed to operate CheMinX. CheMinX has a silicon-drift detector (SDD) to measure fluoresced photons, enabling the quantification of major, minor, and some trace elements via XRF. XRD/XRF data are collected simultaneously in CheMinX, obviating the need for multiple compositional instruments. The CheMinX design also improves upon MSL-CheMin’s sample handling. Instead of sample cells on wheel, which are often not reusable and add complexity in commanding the instrument, CheMinX has single-use cells in a cartridge/dispenser configuration. Because of these improvements, CheMinX is an ideal instrument for Discovery-class life-detection missions, including Mars Life Explorer that was recommended for development in the Planetary Science and Astrobiology Decadal Survey 2023-2032.

E B Rampe↗

A Computational Workflow of Elucidating Viral Impact on Mediating Microbial Response to In-situ Experimental Warming: Bridging microbial modeling to carbon and mineral modeling

Viruses are abundant in soils and shape microbial communities in ways that can potentially influence ecosystem processes, yet their contributions to carbon cycling and mineral transformations remain poorly understood. Here we present a multi-phase framework that links virus-host interactions to soil biogeochemistry by combining ecological simulations, genome- and community-scale metabolic modeling, and statistical and machine-learning analyses. We first calibrated microbial abundance profiles under explicit infection scenarios to capture how viral pressure alters community structure, then explored alternative interaction strategies, including kill-the-winner, piggyback-the-winner, and mixed lytic-lysogenic modes, through forward simulations. These ecological shifts were translated into metabolic consequences using exchange fluxes summarized into biologically meaningful categories, while integrated statistical and machine-learning screens elevated subtle but consistent signals. Application of this framework revealed that viral infections shift the balance between organic and inorganic fluxes, redirecting metabolism from diffuse organic transformations toward inorganic pools such as protons and CO 2 , directly linking viral regulation to respiration and soil carbon balance. The roll-up analysis also isolated perturbations in critical mineral ions, including magnesium, manganese, zinc, and copper, which serve as essential enzymatic cofactors. In piggyback-the-winner scenarios, uptake of these ions was strongly suppressed. Contrasting viral strategies produced distinct community structures and metabolic outcomes, from broad suppression under kill-the-winner dynamics to dramatic redistributions under high-lytic and high-gain lysogenic regimes that collapsed vulnerable microbial populations while promoting opportunists. Together, these results provide a tractable path to trace viral perturbations from host abundance shifts to metabolic flux adjustments and ecosystem-scale processes, offering a practical way to include viruses in earth system models.

54 ENVIRONMENTAL SCIENCES↗

The air mycobiome is decoupled from the soil mycobiome in the California San Joaquin Valley

Abstract Dispersal is a key force in the assembly of fungal communities and the air is the dominant route of dispersal for most fungi. Understanding the dynamics of airborne fungi is important for determining their source and for helping to prevent fungal disease. This understanding is important in the San Joaquin Valley of California, which is home to 4.2 million people and where the airborne fungus Coccidioides is responsible for the most important fungal disease of otherwise healthy humans, coccidioidomycosis. The San Joaquin Valley is the most productive agricultural region in the United States, with the principal crops grown therein susceptible to fungal pathogens. Here, we characterize the fungal community in soil and air on undeveloped and agricultural land in the San Joaquin Valley using metabarcoding of the internal transcribed spacer 2 variable region of fungal rDNA. Using 1,002 individual samples, we report one of the most extensive studies of fungi sampled simultaneously from air and soil using modern sequencing techniques. We find that the air mycobiome in the San Joaquin Valley is distinct from the soil mycobiome, and that the assemblages of airborne fungi from sites as far apart as 160 km are far more similar to one another than to the fungal communities in nearby soils. Additionally, we present evidence that airborne fungi in the San Joaquin Valley are subject to dispersal limitation and cyclical intra‐annual patterns of community composition. Our findings are broadly applicable to understanding the dispersal of airborne fungi and the taxonomic structure of airborne fungal assemblages.

59 BASIC BIOLOGICAL SCIENCES↗

Ecosystem-Level Energy and Water Budgets Are Resilient to Canopy Mortality in Sparse Semiarid Biomes

Climate-driven woody vegetation mortality is a defining feature of semiarid biomes that drives fundamental changes in ecosystem structure. However, the observed impacts of woody mortality on ecosystem-scale energy and water budgets and the responses of surviving vegetation are highly variable among studies in water-limited environments. A previous girdling manipulation experiment in a piñon-juniper woodland suggested that although ecosystem-scale evapotranspiration was not altered by large-scale piñon mortality, soil water content decreased and the surviving juniper experienced greater water stress than juniper in an undisturbed woodland. Here we experimentally explored to what extent mortality-induced changes in energy balance components can explain these results. We compared energy fluxes measured above two adjacent piñon-juniper woodlands where piñon girdling was implemented at one site and the other subsequently experienced large-scale natural piñon mortality. We found that the mortality-induced decrease in canopy area was not sufficient to alter surface reflectance, roughness, and partitioning between energy budget components at both sites. A radiative transfer model estimated that because of the sparse premortality canopy, surface reflectance is more sensitive to a large increase in understory leaf area than further loss of crown area. Increased water stress in the remaining juniper following both mortality events can be explained by an increase in radiation on the ground that promoted higher soil temperature and evaporation. We found similar responses of ecosystem and tree-level functions to both girdling and natural mortality. This suggests that girdling is an appropriate approach to explore the impact of tree mortality on ecosystem structure, function, and energy balance.

54 ENVIRONMENTAL SCIENCES↗

Life cycle Management of 324 Project High-Activity, Mixed/Low-Level Radioactive Waste - 20506

U.S. DOE contractor CH2M Hill Plateau Remediation Company (CHPRC) manages the 324 Building and is preparing to remotely excavate and disposition the radioactive soil beneath the building to allow further deactivation of the building. The 324 Building is a non-reactor Category 2 Nuclear Facility located in the 300 Area of the Hanford Site. Records indicate that in October 1986, approximately 516 liters of a concentrated liquid waste stream containing cesium-137 ({sup 137}Cs) and strontium-90 ({sup 90}Sr) spilled onto the floor of a hot cell, B-Cell, in the 324 Building. The spill contained an estimated 1.3 million curies (Ci) of radioactivity. A breach in the sump of B-Cell was discovered in November 2009 during characterization of the soil under the building. Exposure rates in excess of 10,000 R/hr were detected through a system of access pipes installed under the hot cell, and an estimated 1.557 E+05 Ci of {sup 137}Cs and 6.842 E+04 Ci of {sup 90}Sr are in the soil beneath B-Cell. The magnitude of the soil contamination below B-Cell represents one of the most challenging remediation activities in the DOE complex. The objective of CHPRC's 324 Project is to remotely excavate and disposition the highly contaminated soil under the hot cell so building deactivation and stabilization activities can resume. Depending on the volume and radioactivity of contaminated soil under B-Cell, the material will be dispositioned either at the Hanford Site's Environmental Restoration Disposal Facility (ERDF) or in grout monoliths that will be created in the hot cells (A, C and D) next to B-Cell during future building demolition activities. The current project scope includes installing structural supports under the walls of B-Cell and using remotely operated equipment to remove debris from the hot cell, remove debris mixed with grout on the floor, saw through and remove the cell floor and liner, and excavate contaminated soil under B-Cell to a depth of up to 3.66 meters (12 feet). Addition or removal of debris in A-Cell may be required to support B-Cell cleanout and excavation activities. The base approach is to disposition debris and soil bins in the Radiochemical Engineering Cell (REC) monoliths, and subsequently disposition the monoliths during building demolition. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Characterization and Glass Formation of JSC-1 Lunar and Martian Soil Simulants

The space exploration mission of NASA requires long duration presence of human being beyond the low earth orbit (LEO), especially on Moon and Mars. Developing a human habitat or colony on these planets would require a diverse range of materials, whose applications would range from structural foundations, (human) life support, (electric) power generation to components for scientific instrumentation. A reasonable and cost-effective approach for fabricating the materials needed for establishing a self-sufficient human outpost would be to primarily use local (in situ) resources on these planets. Since ancient times, glass and ceramics have been playing a vital role on human civilization. A long term project on studying the feasibility of developing glass and ceramic materials using Lunar and Martian soil simulants (JSC-1) as developed by Johnson Space Center has been undertaken. The first step in this on-going project requires developing a data base on results that fully characterize the simulants to be used for further investigations. The present paper reports characterization data of both JSC-1 Lunar and JSC Mars-1 simulants obtained up to this time via x-ray diffraction analysis, scanning electron microscopy, thermal analysis (DTA, TGA) and chemical analysis. The critical cooling rate for glass formation for the melts of the simulants was also measured in order to quantitatively assess the glass forming tendency of these melts. The importance of the glasses and ceramics developed using in-situ resources for constructing human habitats on Moon or Mars is discussed.

Sen, Subhayu↗

A hotspot model for leaf canopies

The hotspot effect, which provides important information about canopy structure, is modeled using general principles of environmental physics as driven by parameters of interest in remote sensing, such as leaf size, leaf shape, leaf area index, and leaf angle distribution. Specific examples are derived for canopies of horizontal leaves. The hotspot effect is implemented within the framework of the model developed by Suits (1972) for a canopy of leaves to illustrate what might occur in an agricultural crop. Because the hotspot effect arises from very basic geometrical principles and is scale-free, it occurs similarly in woodlands, forests, crops, rough soil surfaces, and clouds. The scaling principles advanced are also significant factors in the production of image spatial and angular variance and covariance which can be used to assess land cover structure through remote sensing.

Jupp, David L. B.↗

Dynamics of vegetation and soils of oak/saw palmetto scrub after fire: Observations from permanent transects

Ten permanent 15 m transects previously established in two oak/saw palmetto scrub stands burned in December 1986, while two transects remained unburned. Vegetation in the greater than 0.5 m and the less than 0.5 m layers on these transects was sampled at 6, 12, 18, 24, and 36 months postburn and determined structural features of the vegetation (height, percent bare ground, total cover). The vegetation data were analyzed from each sampling by height layer using detrended correspondence analysis ordination. Vegetation data for the greater than 0.5 m layer for the entire time sequence were combined and analyzed using detrended correspondence analysis ordination. Soils were sampled at 6, 12, 18, and 24 months postburn and analyzed for pH, conductivity, organic matter, exchangeable cations (Ca, Mg, K, Na), NO3-N, NH4-N, Al, available metals (Cu, Fe, Mn, Zn), and PO4-P. Shrub species recovered at different rates postfire with saw palmetto reestablishing cover greater than 0.5 m within one year, but the scrub oaks had not returned to preburn cover greater than 0.5 m in 3 years after the fire. These differences in growth rates resulted in dominance shifts after the fire with saw palmetto increasing relative to the scrub oaks. Overall changes in species richness were minor, although changes occurred in species richness by height layers due to different growth rates. Soils of well drained and poorly drained sites differed markedly. Soil responses to the fire appeared minor. Soil pH increased at 6 and 12 months postfire; calcium increased at 6 months postburn. Nitrate-nitrogen increased at 12 months postburn. Low values of conductivity, PO4-P, Mg, K, Na, and Fe at 12 months postburn may be related to heavy rainfall the preceding month. Seasonal variability in some soil parameters appeared to occur.

Schmalzer, Paul A.↗

Functional Redundancy in Soil Microbial Community Based on Metagenomics Across the Globe

Understanding the contribution of soil microbial communities to ecosystem processes is critical for predicting terrestrial ecosystem feedbacks under changing climate. Our current understanding lacks a consistent strategy to formulate the linkage between microbial systems and ecosystem processes due to the presumption of functional redundancy in soil microbes. Here we present a global soil microbial metagenomic analysis to generalize patterns of microbial taxonomic compositions and functional potentials across climate and geochemical gradient. Our analyses show that soil microbial taxonomic composition varies widely in response to climate and soil physicochemical gradients, while microbial functional attributes based on metagenomic gene abundances are redundant. Among 17 climate zones, microbial taxonomic compositions were more distinct than functional potentials, as climate and edaphic properties showed more significant influence on microbial taxonomic compositions than on functional potentials. Microbial taxonomies formed a larger and more complex co-occurrence network with more module structures than functional potentials. Functional network was strongly inter-connected among different categories, whereas taxonomic network was more positively interactive in the same taxonomic groups. This study provides strong evidence to support the hypothesis of functional redundancy in soil microbes, as microbial taxonomic compositions vary to a larger extent than functional potentials based on metagenomic gene abundances in terrestrial ecosystems across the globe.

59 BASIC BIOLOGICAL SCIENCES↗

Pyoverdine binding aptamers and label-free electrochemical detection of pseudomonads

Pyoverdines are iron-chelating siderophores employed by various pseudomonads to promote their growth in iron-limited environments, facilitating both beneficial and detrimental interactions with co-inhabiting microbes or hosts, including plants and animals. The fluorescent pseudomonads produce fluorescent pyoverdines comprised of a conserved central chromophore and a unique strain-specific peptidic side chain produced by non-ribosomal peptide synthetases. Pyoverdine Pf5 (PVD-Pf5) is produced by Pseudomonas protegens Pf-5, a species known for supporting plant growth and its involvement in plant pathogen control. To develop a means of exploring the dynamics of P. protegens activity in soil and in the rhizosphere, we selected DNA aptamers that specifically recognize PVD-Pf5 with high affinities. Two selected aptamers with only 16% identity in sequence were examined for structure and function. We found evidence that both aptamers form structures in their apo-forms and one aptamer has structural features suggesting the presence of a G-quadruplex. Although their tertiary structures are predicted to be different, both aptamers bind the target PVD-Pf5 with similar affinities and do not bind other siderophores, including the related pyoverdine, pseudobactin, produced by Pseudomonas sp. B10. One aptamer binds the pyoverdine peptide component and may also interact with the chromophore. This aptamer was integrated into a nanoporous aluminum oxide biosensor and demonstrated to successfully detect PVD-Pf5 and not to detect other siderophores that do not bind to the aptamer when evaluated in solution. This sensor provides a future opportunity to track the locations of P. protegens around plant roots and to monitor PVD-Pf5 production and movement through the soil.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗