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DOE OSTI2025

Using low cost, bio-derived and recycled materials in advanced scalable small- and medium- wind turbine manufacturing

In partnership with Bergey Windpower, this project explored the use of recyclable and recycled materials in small- to medium-wind turbine blades (WTBs). A recyclable epoxy resin and infusible thermoplastic resin were investigated using identical fiber reinforcements and manufacturing practices used as Bergey, and the substitution of continuous virgin glass fiber (GF) layers with recycled nonwoven GF (rGF, NW) mats was trialed. The alternative resins both showed very promising performance in terms of static tensile and flexural behavior as well as thermal properties and fatigue behavior in comparison with that of the incumbent materials used at Bergey. The NW rGF materials, however, were found to be a poor substitute for the continuous GF materials for two primary reasons. Firstly, the NW mats took up significantly more resin than their continuous counterparts, which would increase both the weight and the cost of the WTBs. Additionally, an unexpected reaction occurred between the rGF and epoxy materials that resulted in a highly porous, foamed structure. The foaming phenomenon was not observed with the rGF NW and infusible thermoplastic material, but the resin uptake was still prohibitively high. Based on the results of this study, recyclable infusible resins could be viable candidate materials for Bergey in the future. The foaming reaction with the rGF NW mats could prove highly useful in other applications requiring structural foams and has been used to produce demonstrative sandwich panels.

17 WIND ENERGY↗

DOE OSTI2025

Demonstration of Monte Carlo Reference Equilibrium Core Solutions for Pebble Bed Reactors Using Shift in kugelpy

This report describes how to produce reference solutions for the equilibrium core search of pebble bed reactors using the pyrates Python library. The pyrates library uses the kugelpy methodology to perform running-in calculations to reach an equilibrium core. This approach relies on full-core Monte Carlo neutron transport calculations and the shifting of fuel pebble inventory through the reactor to simulate pebble movement in the core from one step to the next of a running-in scenario. In addition to the use of Serpent for the Monte Carlo neutron transport calculations, as part of this work, updates for the use of the Shift Monte Carlo code were integrated into the official pyrates GitHub repository. Comparisons of running-in simulations for a generic pebble bed reactor produced by using pyrates with Serpent and Shift are shown. Although consistent trends are shown between the use of the two Monte Carlo codes, the limiting factor in either calculation is the computation time due to the need to perform several hundred full-core neutron transport calculations before an equilibrium core is reached.

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

DOE OSTI2026

NUM-DAT File Format Specification: Used in M-9 Gun Experiment Data Archiving

The M-9 Shock and Detonation Physics group executes experiments on gun and explosive platforms with large numbers of oscilloscopes used for data acquisition. The data acquisition from these oscilloscopes was automated many years ago using a custom piece of software called RunDig . The default save format from this software is a custom structure referred to as "NUM-DAT" format. This file format includes a text ".DAT" file which is a header file used to interpret the binary ".NUM" file which contains the oscilloscope data. The data save format was originally developed by John Vorthman and has been in use by M-9 personnel for over 20 years. This data format has been used for archiving data from experiments performed by M-9 personnel at TA-40, TA-39, and the TA-55 Impact Test Facility. Numerous custom analysis and visualization programs have also been developed, and continue to be used, that utilize this data format. This document describes the NUM-DAT format and provides code examples for reading the format and converting it to other formats.

47 OTHER INSTRUMENTATION↗

DOE OSTI2025

Demonstration of Monte Carlo Reference Equilibrium Core Solutions for Pebble Bed Reactors Using Shift in kugelpy

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

DOE OSTI2025

Evaluating User Errors and Temporal Trends in Marine Fish Communities Using 360-Degree Underwater Photography

The use of environmental DNA (eDNA) sampling has been proposed as a complementary method to monitor fish species in marine environments, offering a non-invasive and potentially more efficient approach to marine species observations. eDNA monitoring could be especially useful in and around sites targeted for marine energy generation as these regions need regular monitoring that would be impractical with traditional techniques. Before we can fully rely upon eDNA, we must first verify its accuracy against other proven methods, such as the use of underwater photography. In this study, I deployed a 360-degree camera in the tidal channel of Sequim Bay once a month during several hours overlapping slack tide. I investigated how having multiple people identify and count fish on underwater images could affect the overall results. Using chi square tests in R, I compared my fish identifications and counts to those made by another intern on the same images recorded in August. I found significant differences in the number of species identified and the total individual counts between the two different datasets. I also tested the statistical differences in both Shannon diversity and Pielou evenness indices between the August, September, and November camera deployments using a Hutcheson t-test. Only one significant difference was found in the Shannon index comparisons, and none were found between the Pielou evenness comparisons. These findings show that if multiple identifiers are used to process underwater images, quality control checks must be made to reduce the potential for error. This also points toward the possibility to leverage more advanced image analysis processes, such as automated image analysis software. The findings from this study also show that the dynamics of marine fish communities can vary over a few months; however, further analysis is needed to determine the extent of the seasonal changes in Sequim Bay.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2026

Development and implementation of high-throughput proteomic and metabolomics assays by using advanced chromatographic and mass spectrometric systems (CRADA Final Report)

The mission of this CRADA with Agilent was to couple powerful MS platforms (QQQ, IM-QTOFMS) with Agilent’s novel Ultra-High-Performance Liquid Chromatography (UHPLC) fast metabolomic workflows and perform ABF Machine Learning (ML) to generated datasets. Agilent transferred UHPLC methods to PNNL and LBNL and methods were implemented and demonstrated in both labs, achieving total acquisition times of < 10 min. Metabolites analyzed using Agilent’s shared methods included metabolites from central carbon metabolism, common across hosts, and metabolites unique to engineered strains. Standards were acquired in an UHPLC-Drift Tube Ion Mobility Mass Spectrometer (DTIMS) system for the first time within the context of ABF and methods were optimized based on Agilent’s protocols. Samples from ABF hosts Pseudomonas putida, Aspergillus pseudoterreus, Aspergillus niger and Rhodosporidium toruloides were analyzed using the UHPLC-DTIMS platform for a total of 276 runs. A data analysis workflow compatible with the Experimental Data Depot (EDD) and completely shareable was developed for the acquired UHPLC-DTIMS data. Samples were analyzed using a Data Independent Acquisition Approach (DIA), which for most of the standards provided more transitions therefore increasing detection confidence. Using the data acquired by PNNL, LBNL, and Agilent’s specifications from previous ML projects, SNL applied an ensemble ML strategy to pick the best performing model for automated LC-method selection. Finally, with the contribution of the participant labs and Agilent, SNL developed an Automated Method Selection (AMS) software tool to predict the best liquid chromatography method for analysis of any new molecules of interest. Samples with novel pathways and new metabolite targets of interest are generated at a high pace in the ABF. Overall, the project advanced rapid metabolomics by combining liquid chromatography, ion mobility spectrometry, and data-independent mass spectrometry with machine learning. This multidimensional approach uses retention time, collision cross-section, precursor mass, and fragment-ion information to distinguish chemically similar metabolites that can be difficult to resolve using conventional liquid- or gas-chromatography methods. The resulting workflow also provided automated metabolite-identification error estimates, addressing a recognized need for statistical confidence measures in metabolomics.

Petzold, Christopher [Lawrence Berkeley National L↗

DOE OSTI2026

Characterization of Oxidative Modifications to Short Peptides Using Low Dose Rate X-Rays

The method of X-ray footprinting and mass spectrometry (XFMS) using high flux synchrotron X-ray sources has become an established method in structural biology and is based on the radiolytic production of hydroxyl radicals, which oxidatively modify protein sidechains. While other methods of producing hydroxyl radicals are available, one benefit of using high flux density sources is that hydroxyl radical scavenging reactions can be minimized, and exposure times kept short to minimize secondary reactions. Here we present an application of the XFMS method using low dose rate X-rays from a commercial instrument. We demonstrate the feasibility of the approach using short peptides, characterizing the oxidative modifications +14, +16, and +32 Da under both aerobic and low oxygen conditions, and we additionally quantify the hydrogen peroxide production for various doses using the low dose rate source. These results provide fundamental information on the oxidative damage to peptides due to hydroxyl radicals using a low dose rate X-ray source.

X-ray methods↗

DOE OSTI2025

Using feature importance as an exploratory data analysis tool on Earth system models

Abstract. Machine learning (ML) models are commonly used to generate predictions, but these models can also support the discovery of new science. Generating accurate predictions necessitates that a model captures the structure of the underlying data. If the structure is properly extracted, ML could be a useful exploratory and evidential tool. In this paper, we present a case study that demonstrates the use of ML for exploratory data analysis (EDA) in the climate space. We apply the ML explainability method of spatiotemporal zeroed feature importance (stZFI) to understand how climate-variable associations evolve over space and time. Our analyses focus on data from ensembles of Earth system models (ESMs) which provide data on different climate states and conditions. We elect to work with ESM ensembles since they allow us to compare feature importance across alternative scenarios not available with observed data. The ensembles also account for natural variability so that we can distinguish between signal and noise due to natural climate variability when computing feature importance. The use of perturbed initial condition ensembles introduces variability mimicking the natural variability in the atmosphere; thus the signals emerging using feature importance (FI) can be evaluated against the natural variability in the climate system. For our analyses, we consider the 1991 volcanic eruption of Mount Pinatubo, which was a large stratospheric aerosol injection. We explore the climate pathway associated with the eruption from aerosols to radiation to temperature at both the near-surface and stratospheric levels. In addition to applying the method to data generated from two different ESMs, we apply stZFI to reanalysis data to compare the associations identified by stZFI. We show how stZFI tracks the importance of aerosol optical depth over time on forecasting temperatures. This case study illustrates usefulness of an ML tool (stZFI) for EDA on a well-studied climate exemplar.

Ries, Daniel (ORCID:0000000250294647)↗

DOE OSTI2026

Evolution of the Antarctic Ice Sheet from 2000–2300 and beyond: model sensitivity and uncertainty analysis using MPAS-Albany Land Ice

We present a description of the Antarctic Ice Sheet model configuration submitted to the ISMIP6-Antarctica-2300 experiment using the MPAS-Albany Land Ice model, along with three new sets of simulations: (1) a set of extended simulations to 2500 for three forced experiments and to 2775 for the control experiment; (2) a sensitivity analysis of our model configuration to parameters controlling basal sliding and sub-shelf melt, and to model structural choices including the choice of the energy and stress balances; and (3) a 72-member ensemble run on graphics processing units (GPUs) and analysis of variance to determine the primary sources of uncertainty in our ice-sheet model projections. Our extended simulations predict rapid retreat beginning after 2300 for SSP1-2.6 forcing and after 2500 for present-day (control) forcing, primarily in the Amundsen Sea Embayment. We find that varying the sub-shelf melt parameter between the 5th to 95th percentile values for a mean-Antarctic calibration target results in an up to ∼ ± 40 % change in sea-level contribution relative to our baseline simulations that used the median value. Using a linear basal sliding law reduces sea-level contribution by 51 %–73 % relative to our baseline nonlinear sliding law with an exponent of 1/5. When using basal sliding law exponents of 1/3 and 1/10, the overall difference from our baseline simulations at 2300 is on the order of 10 %. The Amundsen Sea Embayment region displays a strongly non-linear dependence of mass loss on the sliding law exponent, with no discernible relationship between the sliding law exponent and the mass loss by 2300, while the sectors feeding the Ross and Filchner-Ronne ice shelves exhibit more mass loss with a more-plastic sliding law. Our model fidelity sensitivity experiments reveal a 9 %–31 % increase in sea-level contribution when using a depth-integrated stress balance approximation relative to our three-dimensional solver, while using a fixed-in-time temperature field increases sea-level contribution by 14 %–88 % relative to two thermomechanically coupled configurations. Our 72-member ensemble and analysis of variance show that the uncertainty in long-term projections is dominated by the choice of Earth system model forcing and the presence or absence of hydrofracture forcing, rather than uncertainty in sliding and sub-shelf melt parameters.

58 GEOSCIENCES↗

NASA NTRS1973

Land use mapping and modelling for the Phoenix Quadrangle

The author has identified the following significant results. Comparison of 9 x 9 MSS band images and color composites made from bands 4, 5, and 6 showing vegetated areas near Phoenix during the summer and fall seasons aided in definitely establishing that certain land areas were being used as agricultural land and not as rangeland. Agricultural land, which appeared to be fallow, idle, or not irrigated, often became more readily identifiable as agricultural land when comparing different images of identical land areas which have been affected by seasonal vegetation changes. Experimentation with color density slicing portions of 9 x 9 MSS band 7 transparency showing the central urban core of phoenix enabled dense commercial and industrial areas to be separated from less dense urbanized land uses; however, loss of resolution produced results of limited usefulness. The best results in agricultural areas near Sun City were obtained using MSS band 5 imagery. Discrimination of different land uses in both urban and agricultural areas which were color density sliced was not possible to the degree of accuracy necessary to make mapping feasible. Examination of MSS transparencies and color composites allowed updating of a map of land use change in the Phoenix Quadrangle.

Place, J. L.↗

NASA NTRS1973

Effective use of ERTS multisensor data in the Great Plains

The author has identified the following significant results. In the case of rangelands, manipulation of the digitized material from photographic recordings revealed that the mode-seeking program using only one vegetation group gave the best output map. Efficient mapping of this imagery will possibly require the use of narrow band filters. In the case of cropland, the results obtained with the K-class classifier are shown. Using one feature, the highest percent correct classification was obtained using band 6. Using two features, best results were obtained using bands 4 and 6 and 5 and 6. Specific results obtained with various bands are discussed. In the case of land systems, the eroded shale soils located along the Missouri River reservoirs in South Dakota above the Fort Randall and Big Bend Dams are clearly visible on the IR bands of ERTS scene 17 August, 1972, image description number 1025-16551. The appearance of various types of soil formations is described.

Myers, V. I.↗

NASA NTRS1970

The use of space and high altitude aerial photography to classify forest land and to detect forest disturbances

In October 1969, an investigation was begun near Atlanta, Georgia, to explore the possibilities of developing predictors for forest land and stand condition classifications using space photography. It has been found that forest area can be predicted with reasonable accuracy on space photographs using ocular techniques. Infrared color film is the best single multiband sensor for this purpose. Using the Apollo 9 infrared color photographs taken in March 1969 photointerpreters were able to predict forest area for small units consistently within 5 to 10 percent of ground truth. Approximately 5,000 density data points were recorded for 14 scan lines selected at random from five study blocks. The mean densities and standard deviations were computed for 13 separate land use classes. The results indicate that forest area cannot be separated from other land uses with a high degree of accuracy using optical film density alone. If, however, densities derived by introducing red, green, and blue cutoff filters in the optical system of the microdensitometer are combined with their differences and their ratios in regression analysis techniques, there is a good possibility of discriminating forest from all other classes.

Aldrich, R. C.↗

NASA NTRS1973

ERTS regional scale overview linking land use and environmental processes in CARETS

A mosaic of ERTS images of the CARETS region has been used to partition the region into zones on the basis of similarity of tones and textures visible at a regional-scale overview. The resulting patterns were compared with existing small scale maps of the region representing relief, land surface forms, geology, soils, vegetation, forest types, and land use. The ERTS-derived zones most closely resemble the patterns on the small scale land use map, suggesting that, at least in a highly developed region such as CARETS, land use is an indicator or resultant surface expression of several interacting environmental processes. These results lend support to the CARETS model of interdisciplinary regional analysis, whereby remote sensor-derived data sets on land use and land use change become the basic data entry into a regional information system to serve regional planners and land managers.

Alexander, R. H.↗

NASA NTRS1974

Automated land-use mapping from spacecraft data

The author has identified the following significant results. In response to the need for a faster, more economical means of producing land use maps, this study evaluated the suitability of using ERTS-1 computer compatible tape (CCT) data as a basis for automatic mapping. Significant findings are: (1) automatic classification accuracy greater than 90% is achieved on categories of deep and shallow water, tended grass, rangeland, extractive (bare earth), urban, forest land, and nonforested wet lands; (2) computer-generated printouts by target class provide a quantitative measure of land use; and (3) the generation of map overlays showing land use from ERTS-1 CCTs offers a significant breakthrough in the rate at which land use maps are generated. Rather than uncorrected classified imagery or computer line printer outputs, the processing results in geometrically-corrected computer-driven pen drawing of land categories, drawn on a transparent material at a scale specified by the operator. These map overlays are economically produced and provide an efficient means of rapidly updating maps showing land use.

Chase, P. E.↗

NASA NTRS1974

Remote sensing of land use changes in US metropolitan regions: Techniques of analysis and opportunities for application

A graphic description is given of the Census Cities ERTS experiment in urban change detection using remote sensors. The relationship or model between land use data from sensors and socio-demographic data from the census is partly demonstrated. The example suggests how knowledge of land use changes acquired by sensors can be used to make estimates of population, and other attributes. The feasibility of nationwide mapping of land use, and land use changes, by direct computer classification of ERTS-1 multispectral digital data is also demonstrated. Potential applications in state and regional planning are many, and some are named. But the longer-range gains are likely to be improved understanding by legislators, managers and voters as to what it is that makes the country tick. One of the specific tasks could be the allocation of revenues to be shared.

Wray, J. R.↗

NASA NTRS1974

Use of remote sensing techniques for inventorying and planning utilization of land resources in South Dakota

The basic procedures for interpreting remote sensing imagery to rapidly develop general soils and land use inventories were developed and utilized in Pennington County, South Dakota. These procedures and remote sensing data products were illustrated and explained to many user groups, some of whom are interested in obtaining similar data. The general soils data were integrated with land soils data supplied by the county director of equalization to prepare a land value map. A computer print-out of this map indicating a land value for each quarter section is being used in tax reappraisal of Pennington County. The land use data provided the land use planners with the present use of land in Pennington County. Additional uses of remote sensing applications are also discussed including tornado damage assessment, hail damage evaluation, and presentation of soil and land value information on base maps assembled from ERTS-1 imagery.

Myers, V. I.↗

NASA NTRS1975

Consolidation of fatigue and fatigue-crack-propagation data for design use

Analytical methods developed for consolidation of fatigue and fatigue-crack-propagation data for use in design of metallic aerospace structural components are evaluated. A comprehensive file of data on 2024 and 7075 aluminums, Ti-6Al-4V alloy, and 300M steel was established by obtaining information from both published literature and reports furnished by aerospace companies. Analyses are restricted to information obtained from constant-amplitude load or strain cycling of specimens in air at room temperature. Both fatigue and fatigue-crack-propagation data are analyzed on a statistical basis using a least-squares regression approach. For fatigue, an equivalent strain parameter is used to account for mean stress or stress ratio effects and is treated as the independent variable; cyclic fatigue life is considered to be the dependent variable. An effective stress-intensity factor is used to account for the effect of load ratio on fatigue-crack-propagation and treated as the independent variable. In this latter case, crack-growth rate is considered to be the dependent variable. A two term power function is used to relate equivalent strain to fatigue life, and an arc-hyperbolic-tangent function is used to relate effective stress intensity to crack-growth rate.

Rice, R. C.↗

NASA NTRS1975

CARETS: A prototype regional environmental information system. Volume 6: Cost, accuracy and consistency comparisons of land use maps made from high-altitude aircraft photography and ERTS imagery

Accuracy analyses for the land use maps of the Central Atlantic Regional Ecological Test Site were performed for a 1-percent sample of the area. Researchers compared Level II land use maps produced at three scales, 1:24,000, 1:100,000, and 1:250,000 from high-altitude photography, with each other and with point data obtained in the field. They employed the same procedures to determine the accuracy of the Level I land use maps produced at 1:250,000 from high-altitude photography and color composite ERTS imagery. The accuracy of the Level II maps was 84.9 percent at 1:24,000, 77.4 percent at 1:100,000, and 73.0 percent at 1:250,000. The accuracy of the Level I 1:250,000 maps produced from high-altitude aircraft photography was 76.5 percent and for those produced from ERTS imagery was 69.5 percent. The cost of Level II land use mapping at 1:24,000 was found to be high ($11.93 per km 2 ). The cost of mapping at 1:100,000 ($1.75) was about 2 times as expensive as mapping at 1:250,000 ($.88), and the accuracy increased by only 4.4 percent. Level I land use maps, when mapped from high-altitude photography, were about 4 times as expensive as the maps produced from ERTS imagery, although the accuracy is 7.0 percent greater. The Level I land use category that is least accurately mapped from ERTS imagery is urban and built-up land in the non-urban areas; in the urbanized areas, built-up land is more reliably map

Accuracy↗