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At least 793 records · Page 44

Incomplete radiofrequency ablation induced chemoresistance by up-regulating heat shock protein 70 in hepatocellular carcinoma

Highlights: • iRFA treatment inhibited cisplatin-induced pyroptosis of HCC cells. • Heat shock protein 70 was involved in the suppression of NLRP3 activation. • iRFA treatment decreased the cisplatin sensitivity of HCC through regulating HSP70. Radiofrequency ablation (RFA) is a common minimally invasive treatment for hepatocellular carcinoma (HCC). Incomplete RFA (iRFA) due to the sub-lethal heat shock challenge of some cell populations leads to the generation of transformed survivor cells with enhanced chemoresistance. However, the underlying mechanism of iRFA on HCCs chemoresistance remains unknown. In the present study, we investigated the effect of iRFA on HCCs sensitivity to cisplatin. Cells treated with the sub-lethal heat shock challenge were used to mimic iRFA treatment in vitro. An orthotopic implantation HCC model was established and also performed iRFA treatment. Flow cytometry, transwell assay, and cell counting kit-8 assay were used to determine the effect of iRFA treatment on cisplatin-induced HCC cell apoptosis, invasion, and cell viability. ELISA and Western blot were used to detect the effect of iRFA treatment on cisplatin-induced HCC cell pyroptosis. We found that iRFA treatment increased the HCC cell proliferation and invasion ability, and inhibited cisplatin-induced pyroptosis. Further experiments showed that iRFA treatment induced upregulation of HSP70, which inhibited the cisplatin-induced NLRP3 inflammasome activation, leading to inhibition of pyroptosis. HSP70 knockdown or NLRP3 overexpression could reverse the effect of iRFA treatment in vitro. In vivo, HSP70 knockdown reversed the chemosensitivity of HCC to cisplatin, which was decreased by iRFA. In conclusion, we demonstrated that iRFA induced drug resistance by HSP70-mediated inhibition of cell pyroptosis in HCC.

60 APPLIED LIFE SCIENCES↗

Learning the Low Frequency Earthquake Activity on the Central San Andreas Fault

Abstract Low frequency earthquakes (LFEs) originating below the central San Andreas Fault are associated with slow‐slip beneath the seismogenic zone within the more ductile portion of the crust. Monitoring efforts over 15 years detected >1 million LFEs. We train a gradient boosted tree model using statistical features describing the seismic waveforms to estimate the hourly LFE event count. The burst‐like LFE behavior is reproduced, while lower amplitudes are predicted during the most active periods. The hourly event counts are up to 18% greater than the catalog. The ability to continuously monitor LFE activity provides insight to when geodetic measurements of slow slip are possible, without the need for developing a computational‐intensive template‐matching catalog. Similar waveform statistical features are found between detecting LFEs and tremors, which provides additional evidence tremors are composed of LFEs. The approach extracts information contained in continuous seismic waveforms that might benefit detecting precursory signals.

58 GEOSCIENCES↗

Planck 2015 results: XXIV. Cosmology from Sunyaev-Zeldovich cluster counts

In this work, we present cluster counts and corresponding cosmological constraints from the Planck full mission data set. Our catalogue consists of 439 clusters detected via their Sunyaev-Zeldovich (SZ) signal down to a signal-to-noise ratio of 6, and is more than a factor of 2 larger than the 2013 Planck cluster cosmology sample. The counts are consistent with those from 2013 and yield compatible constraints under the same modelling assumptions. Taking advantage of the larger catalogue, we extend our analysis to the two-dimensional distribution in redshift and signal-to-noise. We use mass estimates from two recent studies of gravitational lensing of background galaxies by Planck clusters to provide priors on the hydrostatic bias parameter, (1-b). In addition, we use lensing of cosmic microwave background (CMB) temperature fluctuations by Planck clusters as an independent constraint on this parameter. These various calibrations imply constraints on the present-day amplitude of matter fluctuations in varying degrees of tension with those from the Planck analysis of primary fluctuations in the CMB; for the lowest estimated values of (1-b) the tension is mild, only a little over one standard deviation, while it remains substantial (3.7σ) for the largest estimated value. We also examine constraints on extensions to the base flat ΛCDM model by combining the cluster and CMB constraints. The combination appears to favour non-minimal neutrino masses, but this possibility does little to relieve the overall tension because it simultaneously lowers the implied value of the Hubble parameter, thereby exacerbating the discrepancy with most current astrophysical estimates. In conclusion, improving the precision of cluster mass calibrations from the current 10%-level to 1% would significantly strengthen these combined analyses and provide a stringent test of the base ΛCDM model.

79 ASTRONOMY AND ASTROPHYSICS↗

Towards an Introspective Dynamic Model of Globally Distributed Computing Infrastructures

Large-scale scientific collaborations like ATLAS, Belle II, CMS, DUNE, and others involve hundreds of research institutes and thousands of researchers spread across the globe. These experiments generate petabytes of data, with volumes soon expected to reach exabytes. Consequently, there is a growing need for computation, including structured data processing from raw data to consumer-ready derived data, extensive Monte Carlo simulation campaigns, and a wide range of end-user analysis. To manage these computational and storage demands, centralized workflow and data management systems are implemented. However, decisions regarding data placement and payload allocation are often made disjointly and via heuristic means. A significant obstacle in adopting more effective heuristic or AI-driven solutions is the absence of a quick and reliable introspective dynamic model to evaluate and refine alternative approaches. In this study, we aim to develop such an interactive system using real-world data. By examining job execution records from the PanDA workflow management system, we have pinpointed key performance indicators such as queuing time, error rate, and the extent of remote data access. The dataset includes five months of activity. Additionally, we are creating a generative AI model to simulate time series of payloads, which incorporate visible features like category, event count, and submitting group, as well as hidden features like the total computational load—derived from existing PanDA records and computing site capabilities. These hidden features, which are not visible to job allocators, whether heuristic or AI-driven, influence factors such as queuing times and data movement.

kilic, Ozgur Ozan [Brookhaven National Laboratory ↗

Accuracy of maximum likelihood estimates of a two-state model in single-molecule FRET

Photon sequences from single-molecule Förster resonance energy transfer (FRET) experiments can be analyzed using a maximum likelihood method. Parameters of the underlying kinetic model (FRET efficiencies of the states and transition rates between conformational states) are obtained by maximizing the appropriate likelihood function. In addition, the errors (uncertainties) of the extracted parameters can be obtained from the curvature of the likelihood function at the maximum. We study the standard deviations of the parameters of a two-state model obtained from photon sequences with recorded colors and arrival times. The standard deviations can be obtained analytically in a special case when the FRET efficiencies of the states are 0 and 1 and in the limiting cases of fast and slow conformational dynamics. These results are compared with the results of numerical simulations. The accuracy and, therefore, the ability to predict model parameters depend on how fast the transition rates are compared to the photon count rate. In the limit of slow transitions, the key parameters that determine the accuracy are the number of transitions between the states and the number of independent photon sequences. In the fast transition limit, the accuracy is determined by the small fraction of photons that are correlated with their neighbors. The relative standard deviation of the relaxation rate has a “chevron” shape as a function of the transition rate in the log-log scale. The location of the minimum of this function dramatically depends on how well the FRET efficiencies of the states are separated.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

A COMPLETE SAMPLE OF ULTRALUMINOUS X-RAY SOURCE HOST GALAXIES

One hundred seven ultraluminous X-ray sources (ULXs) with 0.3-10.0 keV luminosities in excess of 10{sup 39} erg s{sup -1} are identified in a complete sample of 127 nearby galaxies. The sample includes all galaxies within 14.5 Mpc above the completeness limits of both the Uppsala Galaxy Catalogue and the Infrared Astronomical Satellite survey. The galaxy sample spans all Hubble types, a four-decade range in mass, 7.5 < log (M/M{sub sun}) < 11.4, and in star formation rate, 0.0002 < SFR(M{sub sun} yr{sup -1}) {<=} 3.6. ULXs are detected in this sample at rates of one per 3.2 Multiplication-Sign 10{sup 10} M{sub sun}, one per {approx}0.5 M{sub sun} yr{sup -1} star formation rate, and one per 57 Mpc{sup 3} corresponding to a luminosity density of {approx}2 Multiplication-Sign 10{sup 37} erg s{sup -1} Mpc{sup -3}. At these rates we estimate as many as 19 additional ULXs remain undetected in fainter dwarf galaxies within the survey volume. An estimated 14 objects, or 13%, of the 107 ULX candidates are expected to be background sources. The differential ULX luminosity function shows a power-law slope {alpha} {approx} -0.8 to -2.0 with an exponential cutoff at {approx}20 Multiplication-Sign 10{sup 39} erg s{sup -1} with precise values depending on the model and on whether the ULX luminosities are estimated from their observed numbers of counts or, for a subset of candidates, from their spectral shapes. Extrapolating the observed luminosity function predicts at most one very luminous ULX, L{sub X} {approx} 10{sup 41} erg s{sup -1}, within a distance as small as 100 Mpc. The luminosity distribution of ULXs within the local universe cannot account for the recent claims of luminosities in excess of 2 Multiplication-Sign 10{sup 41} erg s{sup -1}, requiring a new population class to explain these extreme objects.

79 ASTRONOMY AND ASTROPHYSICS↗

DISCONNECTING OPEN SOLAR MAGNETIC FLUX

Disconnection of open magnetic flux by reconnection is required to balance the injection of open flux by coronal mass ejections and other eruptive events. Making use of recent advances in heliospheric background subtraction, we have imaged many abrupt disconnection events. These events produce dense plasma clouds whose distinctive shape can now be traced from the corona across the inner solar system via heliospheric imaging. The morphology of each initial event is characteristic of magnetic reconnection across a current sheet, and the newly disconnected flux takes the form of a 'U-'shaped loop that moves outward, accreting coronal and solar wind material. We analyzed one such event on 2008 December 18 as it formed and accelerated at 20 m s{sup -2} to 320 km s{sup -1}, thereafter expanding self-similarly until it exited our field of view 1.2 AU from the Sun. From acceleration and photometric mass estimates we derive the coronal magnetic field strength to be 8 {mu}T, 6 R{sub Sun} above the photosphere, and the entrained flux to be 1.6 Multiplication-Sign 10{sup 11} Wb (1.6 Multiplication-Sign 10{sup 19} Mx). We model the feature's propagation by balancing inferred magnetic tension force against accretion drag. This model is consistent with the feature's behavior and accepted solar wind parameters. By counting events over a 36 day window, we estimate a global event rate of 1 day{sup -1} and a global solar minimum unsigned flux disconnection rate of 6 Multiplication-Sign 10{sup 13} Wb yr{sup -1} (6 Multiplication-Sign 10{sup 21} Mx yr{sup -1}) by this mechanism. That rate corresponds to {approx} - 0.2 nT yr{sup -1} change in the radial heliospheric field at 1 AU, indicating that the mechanism is important to the heliospheric flux balance.

79 ASTRONOMY AND ASTROPHYSICS↗

X-RAY PROPERTIES OF INTERMEDIATE-MASS BLACK HOLES IN ACTIVE GALAXIES. III. SPECTRAL ENERGY DISTRIBUTION AND POSSIBLE EVIDENCE FOR INTRINSICALLY X-RAY-WEAK ACTIVE GALACTIC NUCLEI

We present a systematic X-ray study, the third in a series, of 49 active galactic nuclei with intermediate-mass black holes (IMBH; {approx}10{sup 5}-10{sup 6} M{sub Sun }) using Chandra observations. We detect 42 out of 49 targets with a 0.5-2 keV X-ray luminosity 10{sup 41}-10{sup 43} erg s{sup -1}. We perform spectral fitting for the 10 objects with enough counts (>200), and they are all well fit by a simple power-law model modified by Galactic absorption, with no sign of significant intrinsic absorption. While we cannot fit the X-ray spectral slope directly for the rest of the sample, we estimate it from the hardness ratio and find a range of photon indices consistent with those seen in more luminous and massive objects. The X-ray-to-optical spectral slope ({alpha}{sub ox}) of our IMBH sample is systematically flatter than in active galaxies with more massive black holes, consistent with the well-known correlation between {alpha}{sub ox} and UV luminosity. Thanks to the wide dynamic range of our sample, we find evidence that {alpha}{sub ox} increases with decreasing M{sub BH} as expected from accretion disk models, where the UV emission systematically decreases as M{sub BH} decreases and the disk temperature increases. We also find a long tail toward low {alpha}{sub ox} values. While some of these sources may be obscured, given the high L{sub bol}/L{sub Edd} values in the sample, we argue that some may be intrinsically X-ray-weak, perhaps owing to a rare state that radiates very little coronal emission.

79 ASTRONOMY AND ASTROPHYSICS↗

SEDS: THE SPITZER EXTENDED DEEP SURVEY. SURVEY DESIGN, PHOTOMETRY, AND DEEP IRAC SOURCE COUNTS

The Spitzer Extended Deep Survey (SEDS) is a very deep infrared survey within five well-known extragalactic science fields: the UKIDSS Ultra-Deep Survey, the Extended Chandra Deep Field South, COSMOS, the Hubble Deep Field North, and the Extended Groth Strip. SEDS covers a total area of 1.46 deg{sup 2} to a depth of 26 AB mag (3{sigma}) in both of the warm Infrared Array Camera (IRAC) bands at 3.6 and 4.5 {mu}m. Because of its uniform depth of coverage in so many widely-separated fields, SEDS is subject to roughly 25% smaller errors due to cosmic variance than a single-field survey of the same size. SEDS was designed to detect and characterize galaxies from intermediate to high redshifts (z = 2-7) with a built-in means of assessing the impact of cosmic variance on the individual fields. Because the full SEDS depth was accumulated in at least three separate visits to each field, typically with six-month intervals between visits, SEDS also furnishes an opportunity to assess the infrared variability of faint objects. This paper describes the SEDS survey design, processing, and publicly-available data products. Deep IRAC counts for the more than 300,000 galaxies detected by SEDS are consistent with models based on known galaxy populations. Discrete IRAC sources contribute 5.6 {+-} 1.0 and 4.4 {+-} 0.8 nW m{sup -2} sr{sup -1} at 3.6 and 4.5 {mu}m to the diffuse cosmic infrared background (CIB). IRAC sources cannot contribute more than half of the total CIB flux estimated from DIRBE data. Barring an unexpected error in the DIRBE flux estimates, half the CIB flux must therefore come from a diffuse component.

79 ASTRONOMY AND ASTROPHYSICS↗

Simulating plasma wave propagation on a superconducting quantum chip

Quantum computers may one day enable the efficient simulation of strongly coupled plasmas that lie beyond the reach of classical computation in regimes where quantum effects are important and the scale separation is large. Here, in this article, we take a first step toward efficient simulation of quantum plasmas by demonstrating linear plasma wave propagation on a superconducting quantum chip. Using high-fidelity and highly expressive device-native gates, combined with an error-mitigation technique, we simulate the scattering of laser pulses from inhomogeneous plasmas. Our approach is made feasible by the identification of a suitable local spin model whose excitations mimic plasma waves, and whose circuit implementation requires a lower gate count than other proposed approaches that would require a future fault-tolerant quantum computer. This work opens avenues to study more complicated phenomena that cannot be simulated efficiently on classical computers, such as nonlinear quantum dynamics when strongly coupled plasmas are driven out of equilibrium.

general physics↗

Correlation-aware binning for small-angle neutron scattering via Gaussian-process inference

Binning in small-angle neutron scattering (SANS) is typically performed empirically, with fixed parameters chosen for convenience rather than statistical optimality. Such practices often fail to balance statistical precision and spatial resolution, leading to inconsistencies across instruments and datasets. Here we establish a correlation-aware framework that determines the optimal bin width from first principles by extending the classical Freedman–Diaconis (FD) rule to account for inter-bin correlations with a Gaussian process. In this formulation, the scattering intensity is treated as a smooth stochastic field whose statistical coherence is described by a covariance matrix. Analytical expressions of errors derived from this model yield closed-form criteria that separate the total deviation into contributions from counting noise, aliasing distortion and curvature-dependent correlation effects. Expressed in reduced variables, the resulting dimensionless error surface reveals a continuous transition from the uncorrelated FD regime to the correlation-dominated limit, providing a unified description of noise suppression and resolution control. Because the formulation depends only on the profile characteristics of scattering intensity I(Q), specifically its average intensity and first- and second-order derivatives, it applies generally to any SANS measurement regardless of sample, instrument or geometry. Experimental validation using small- and ultra-small-angle neutron scattering data confirms the predicted scaling behavior, demonstrating that correlation-aware inference systematically reduces mean-squared error and enables information-efficient reproducible data reduction across materials and instruments.

Tung, Chi-Huan [ORNL] (ORCID:0000000221972074)↗

High–throughput measurement of plant fitness traits with an object detection method using Faster R–CNN

Revealing the contributions of genes to plant phenotype is frequently challenging because loss-of-function effects may be subtle or masked by varying degrees of genetic redundancy. Such effects can potentially be detected by measuring plant fitness, which reflects the cumulative effects of genetic changes over the lifetime of a plant. However, fitness is challenging to measure accurately, particularly in species with high fecundity and relatively small propagule sizes such as Arabidopsis thaliana. An image segmentation-based method using the software ImageJ and an object detection-based method using the Faster Region-based Convolutional Neural Network (R-CNN) algorithm were used for measuring two Arabidopsis fitness traits: seed and fruit counts. The segmentation-based method was error-prone (correlation between true and predicted seed counts, r 2 = 0.849) because seeds touching each other were undercounted. By contrast, the object detection-based algorithm yielded near perfect seed counts (r 2 = 0.9996) and highly accurate fruit counts (r 2 = 0.980). Comparing seed counts for wild-type and 12 mutant lines revealed fitness effects for three genes; fruit counts revealed the same effects for two genes. Our study provides analysis pipelines and models to facilitate the investigation of Arabidopsis fitness traits and demonstrates the importance of examining fitness traits when studying gene functions.

59 BASIC BIOLOGICAL SCIENCES↗

IMAGING ANALYSIS OF THE HARD X-RAY TELESCOPE ProtoEXIST2 AND NEW TECHNIQUES FOR HIGH-RESOLUTION CODED-APERTURE TELESCOPES

Wide-field (≳100 deg{sup 2}) hard X-ray coded-aperture telescopes with high angular resolution (≲2′) will enable a wide range of time domain astrophysics. For instance, transient sources such as gamma-ray bursts can be precisely localized without the assistance of secondary focusing X-ray telescopes to enable rapid followup studies. On the other hand, high angular resolution in coded-aperture imaging introduces a new challenge in handling the systematic uncertainty: the average photon count per pixel is often too small to establish a proper background pattern or model the systematic uncertainty in a timescale where the model remains invariant. We introduce two new techniques to improve detection sensitivity, which are designed for, but not limited to, a high-resolution coded-aperture system: a self-background modeling scheme which utilizes continuous scan or dithering operations, and a Poisson-statistics based probabilistic approach to evaluate the significance of source detection without subtraction in handling the background. We illustrate these new imaging analysis techniques in high resolution coded-aperture telescope using the data acquired by the wide-field hard X-ray telescope ProtoEXIST2 during a high-altitude balloon flight in fall 2012. We review the imaging sensitivity of ProtoEXIST2 during the flight, and demonstrate the performance of the new techniques using our balloon flight data in comparison with a simulated ideal Poisson background.

79 ASTRONOMY AND ASTROPHYSICS↗

The NuSTAR Serendipitous Survey: Hunting for the Most Extreme Obscured AGN at >10 keV

We identify sources with extremely hard X-ray spectra (i.e., with photon indices of Γ≲0.6) in the 13 deg{sup 2} NuSTAR serendipitous survey, to search for the most highly obscured active galactic nuclei (AGNs) detected at >10 keV. Eight extreme NuSTAR sources are identified, and we use the NuSTAR data in combination with lower-energy X-ray observations (from Chandra, Swift XRT, and XMM-Newton) to characterize the broadband (0.5–24 keV) X-ray spectra. We find that all of the extreme sources are highly obscured AGNs, including three robust Compton-thick (CT; N{sub H}>1.5×10{sup 24} cm{sup −2}) AGNs at low redshift (z<0.1) and a likely CT AGN at higher redshift (z = 0.16). Most of the extreme sources would not have been identified as highly obscured based on the low-energy (<10 keV) X-ray coverage alone. The multiwavelength properties (e.g., optical spectra and X-ray–mid-IR luminosity ratios) provide further support for the eight sources being significantly obscured. Correcting for absorption, the intrinsic rest-frame 10–40 keV luminosities of the extreme sources cover a broad range, from ≈5×10{sup 42} to 10{sup 45} erg s{sup −1}. The estimated number counts of CT AGNs in the NuSTAR serendipitous survey are in broad agreement with model expectations based on previous X-ray surveys, except for the lowest redshifts (z<0.07), where we measure a high CT fraction of f{sub CT}{sup obs}=30{sub −12}{sup +16}%. For the small sample of CT AGNs, we find a high fraction of galaxy major mergers (50% ± 33%) compared to control samples of “normal” AGNs.

79 ASTRONOMY AND ASTROPHYSICS↗

Discrimination of growth and water stress in wheat by various vegetation indices through a clear a turbid atmosphere

Reflectance data were obtained over a drought-stressed and a well-watered wheat plot with a hand-held radiometer having bands similar to the MSS bands of the LANDSAT satellites. Data for 48 clear days were interpolated to yield reflectance values for each day of the growing season, from planting until harvest. With an atmospheric path radiance model and LANDSAT-2 calibration data, the reflectance were used to simulate LANDSAT digital counts (not quantized) for the four LANDSAT bands for each day of the growing season, through a clear (approximately 100 km meteorological range) and a turbid (approximately 10 km meteorological range) atmosphere. Several ratios and linear combinations of bands were calculated using the simulated data, then assessed for their relative ability to discriminate vegetative growth and plant stress through the two atmospheres. The results show that water stress was not detected by any of the indices until after growth was retarded, and the sensitivity of the various indices to vegetation depended on plant growth stage and atmospheric path radiance.

Jackson, R. D.↗

Dark Matter near the Sun: Simulated Star Counts and the Oort Limit

An ensemble of orbits passing through the solar position was generated for a specific mass model of the galaxy. These orbits are randomly sampled to form simulated density distributions of tracer stars perpendicular to the galactic disk. The simulated distributions are analyzed to determine the sampling errors in a self consistent derivation of the total amount of matter near the Sun (the Oort limit).

Gilden, D.↗

Efficient design of CMOS TSC checkers

This paper considers the design of an efficient, robustly testable, CMOS Totally Self-Checking (TSC) Checker for k-out-of-2k codes. Most existing implementations use primitive gates and assume the single stuck-at fault model. The self-testing property has been found to fail for CMOS TSC checkers under the stuck-open fault model due to timing skews and arbitrary delays in the circuit. A new four level design using CMOS primitive gates (NAND, NOR, INVERTERS) is presented. This design retains its properties under the stuck-open fault model. Additionally, this method offers an impressive reduction (greater than 70 percent) in gate count, gate inputs, and test set size when compared to the existing method. This implementation is easily realizable and is based on Anderson's technique. A thorough comparative study has been made on the proposed implementation and Kundu's implementation and the results indicate that the proposed one is better than Kundu's in all respects for k-out-of-2k codes.

Biddappa, Anita↗

Leonid Storm Flux Analysis From One Leonid MAC Video AL50R

A detailed meteor flux analysis is presented of a seventeen-minute portion of one videotape, collected on November 18, 1999, during the Leonid Multi-instrument Aircraft Campaign. The data was recorded around the peak of the Leonid meteor storm using an intensified CCD camera pointed towards the low southern horizon. Positions of meteors on the sky were measured. These measured meteor distributions were compared to a Monte Carlo simulation, which is a new approach to parameter estimation for mass ratio and flux. Comparison of simulated flux versus observed flux levels, seen between 1:50:00 and 2:06:41 UT, indicate a magnitude population index of r = 1.8 +/- 0.1 and mass ratio of s = 1.64 +/- 0.06. The average spatial density of the material contributing to the Leonid storm peak is measured at 0.82 +/- 0.19 particles per square kilometer per hour for particles of at least absolute visual magnitude +6.5. Clustering analysis of the arrival times of Leonids impacting the earth's atmosphere over the total observing interval shows no enhancement or clumping down to time scales of the video frame rate. This indicates a uniformly random temporal distribution of particles in the stream encountered during the 1999 epoch. Based on the observed distribution of meteors on the sky and the model distribution, recommendations am made for the optimal pointing directions for video camera meteor counts during future ground and airborne missions.

Gural, Peter S.↗