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At least 793 records · Page 44

In-Field Whole-Plant Maize Architecture Characterized by Subcanopy Rovers and Latent Space Phenotyping

Core Ideas * Subcanopy rovers enabled 3D characterization of thousands of hybrid maize plots. * Machine learning produces heritable latent traits that describe plant architecture. * Rover-based phenotyping is far more efficient than manual phenotyping. * Latent phenotypes from rovers are ready for application to plant biology and breeding. Collecting useful, interpretable, and biologically relevant phenotypes in a resource-efficient manner is a bottleneck to plant breeding, genetic mapping, and genomic prediction. Autonomous and affordable subcanopy rovers are an efficient and scalable way to generate sensor-based datasets of in-field crop plants. Rovers equipped with lidar can produce three-dimensional reconstructions of entire hybrid maize (Zea mays L.) fields. In this study, we collected 2103 lidar scans of hybrid maize field plots and extracted phenotypic data from them by latent space phenotyping. We performed latent space phenotyping by two methods, principal component analysis and a convolutional autoencoder, to extract meaningful, quantitative latent space phenotypes (LSPs) describing whole-plant architecture and biomass distribution. The LSPs had heritabilities of up to 0.44, similar to some manually measured traits, indicating that they can be selected on or genetically mapped. Manually measured traits can be successfully predicted by using LSPs as explanatory variables in partial least squares regression, indicating that the LSPs contain biologically relevant information about plant architecture. These techniques can be used to assess crop architecture at a reduced cost and in an automated fashion for breeding, research, or extension purposes, as well as to create or inform crop growth models.

59 BASIC BIOLOGICAL SCIENCES↗

CHANGE OF MAGNETIC FIELD-GAS ALIGNMENT AT THE GRAVITY-DRIVEN ALFVÉNIC TRANSITION IN MOLECULAR CLOUDS: IMPLICATIONS FOR DUST POLARIZATION OBSERVATIONS

Diffuse striations in molecular clouds are preferentially aligned with local magnetic fields, whereas dense filaments tend to be perpendicular to them. When and why this transition occurs remain uncertain. To explore the physics behind this transition, we compute the histogram of relative orientation (HRO) between the density gradient and the magnetic field in three-dimensional magnetohydrodynamic (MHD) simulations of prestellar core formation in shock-compressed regions within giant molecular clouds. We find that, in the magnetically dominated (sub-Alfvénic) post-shock region, the gas structure is preferentially aligned with the local magnetic field. For overdense sub-regions with super-Alfvénic gas, their elongation becomes preferentially perpendicular to the local magnetic field. The transition occurs when self-gravitating gas gains enough kinetic energy from the gravitational acceleration to overcome the magnetic support against the cross-field contraction, which results in a power-law increase of the field strength with density. Similar results can be drawn from HROs in projected two-dimensional maps with integrated column densities and synthetic polarized dust emission. We quantitatively analyze our simulated polarization properties, and interpret the reduced polarization fraction at high column densities as the result of increased distortion of magnetic field directions in trans- or super-Alfvénic gas. Furthermore, we introduce measures of the inclination and tangledness of the magnetic field along the line of sight as the controlling factors of the polarization fraction. Observations of the polarization fraction and angle dispersion can therefore be utilized in studying local magnetic field morphology in star-forming regions.

79 ASTRONOMY AND ASTROPHYSICS↗

Dynamics and morphology of Beaufort Sea ice determined from satellites, aircraft, and drifting stations

A series of measurements from drifting stations, aircraft, the ERTS-1, Nimbus 4, and Nimbus 5 satellites have jointly provided a new description of the dynamics and morphology of the ice cover of the Beaufort Sea. The combined analysis of these data show that the eastern Beaufort Sea ice cover is made up of large multiyear floes while the western part is made of small, predominantly first-year floes. The analysis suggests that this distribution might be quasi-steady and that the dynamics and thermodynamics of the region are more complex than hitherto known. The measurements consist of: (1) high resolution ERTS-1 imagery which is used to describe floe size and shape distribution, short term floe dynamics, and lead and polynya dynamics; (2) tracking by Nimbus 4 of IRLS drifting buoys to provide ice drift information which enhances the interpretation of the ERTS-1 imagery; (3) Nimbus 5 microwave (1.55 cm wavelength) imagery which provides synoptic, sequential maps on the distribution of multiyear and first-year ice types; (4) airborne microwave surveys and surface based observations made during 1971 and 1972 in conjunction with the AIDJEX (Arctic Ice Dynamics Joint Experiment) program.

Campbell, W. J.↗

LANDSAT C workshop field/laboratory exercises

Collection of ground information and supportive materials are absolutely necessary to verify and substantiate data extracted by the interpretation process regardless of sensor type and scale. Field observation and notes, the use of topographic and thematic maps, crop calendars, and climate records are just four examples of supportive materials which often are used in conjunction with remotely sensed materials. Illustration of this integrated multisensor approach is provided by four examples from the March, 1978 Santa Maria LANDSAT C Conference and Workshop. Four distinctive window sites were selected to demonstrate the usefulness of remotely sensed materials to solve geographic problems.

Frasca, J. W.↗

LANDSAT data for coastal zone management

The lack of adequate, current data on land and water surface conditions in New Jersey led to the search for better data collections and analysis techniques. Four-channel MSS data of Cape May County and access to the OSER computer interpretation system were provided by NASA. The spectral resolution of the data was tested and a surface cover map was produced by going through the steps of supervised classification. Topics covered include classification; change detection and improvement of spectral and spatial resolution; merging LANDSAT and map data; and potential applications for New Jersey.

Mckenzie, S.↗

Far IR Observations of the Magellanic Clouds

Several Large Magellanic and Small Magellanic Cloud H II regions were observed at 50 and 100 microns. Observations were made on three flights of the Kuiper Airborne Observatory using a six channel detector system. Fully sampled maps were made simultaneously at both wavelengths. The integrated properties of the H II regions are listed and interpretations of the results regarding stellar formation are presented.

Jones, T. J.↗

The Nimbus 7 LIMS map archival tape

The development of the Langley Map Archival Tape (LAMAT) is described. The role of the Nimbus 7 orbit characteristics in the analysis and interpretation of LAMAT data is discussed. The pressure ranges and heights for the LAMAT components are given and the synoptic fields for each pressure and latitude were modeled as Fourier series in longitude with time-dependent coefficients. Examples of LAMAT temperature fields are presented and the accuracies of the LAMAT fields are evaluated. The fit between the Langley inverted profile archival tape (LAIPAT) and LAMAT is examined by comparing averaged zonal mean fields and standard deviations. Consideration is given to diurnal variations of LAIPAT data, and the time and spatial smoothing of the data.

Haggard, K. V.↗

A summary of image segmentation techniques

Machine vision systems are often considered to be composed of two subsystems: low-level vision and high-level vision. Low level vision consists primarily of image processing operations performed on the input image to produce another image with more favorable characteristics. These operations may yield images with reduced noise or cause certain features of the image to be emphasized (such as edges). High-level vision includes object recognition and, at the highest level, scene interpretation. The bridge between these two subsystems is the segmentation system. Through segmentation, the enhanced input image is mapped into a description involving regions with common features which can be used by the higher level vision tasks. There is no theory on image segmentation. Instead, image segmentation techniques are basically ad hoc and differ mostly in the way they emphasize one or more of the desired properties of an ideal segmenter and in the way they balance and compromise one desired property against another. These techniques can be categorized in a number of different groups including local vs. global, parallel vs. sequential, contextual vs. noncontextual, interactive vs. automatic. In this paper, we categorize the schemes into three main groups: pixel-based, edge-based, and region-based. Pixel-based segmentation schemes classify pixels based solely on their gray levels. Edge-based schemes first detect local discontinuities (edges) and then use that information to separate the image into regions. Finally, region-based schemes start with a seed pixel (or group of pixels) and then grow or split the seed until the original image is composed of only homogeneous regions. Because there are a number of survey papers available, we will not discuss all segmentation schemes. Rather than a survey, we take the approach of a detailed overview. We focus only on the more common approaches in order to give the reader a flavor for the variety of techniques available yet present enough details to facilitate implementation and experimentation.

Spirkovska, Lilly↗

Distribution of Hydrogen Peroxide, Carbon Dioxide, and Sulfuric Acid in Europa's Icy Crust

Galileo's Near Infrared Mapping Spectrometer (NIMS) detected hydrogen peroxide, carbon dioxide and a hydrated material on Europa's surface, the latter interpreted as hydrated sulfuric acid (H2SO4*nH2O) or hydrated salts. Related compounds are molecular oxygen, sulfur dioxide, and two chromophores, one that is dark in the ultraviolet(UV) and concentrated on the trailing side, the other brighter in the UV and preferentially distributed in the leading hemisphere. The UV-dark material has been suggested to be sulfur.

Carlson, R. W.↗

Evidence for Basinwide Mud Volcanism in Acidalia Planitia, Mars

High-albedo mounds in Acidalia Planitia occur in enormous numbers. They have been variously interpreted as pseudocraters, cinder cones, tuff cones, pingos, ice disintegration features, or mud volcanoes. Our work uses regional mapping, basin analysis, and new data from the Context Camera (CTX), High Resolution Imaging Science Experiment (HiRISE), and Compact Reconnaissance Imaging Spectrometer for Mars (CRISM) to re-assess the origin and significance of these structures.

Oehler, Dorothy Z.↗

Toa Baja Disasters: Utilizing Open-Source Earth Observations to Inform the Toa Baja Municipality’s Flood Mitigation Efforts and Educate the Public

Toa Baja, located just west of San Juan in Puerto Rico, is known as “the underwater city” due to its propensity to flood. The city contains the mouth of the island’s longest river, Río de la Plata, which drains into the Atlantic Ocean on the northern edge of the municipality. Proximity to these major water features and the flat, low terrain contribute to the flood-prone nature of the area. During tropical storm events, such as Hurricane Maria in 2017, Toa Baja experienced inundation of up to 20 feet. Changes in the global climate system are causing more intense and frequent tropical storms, making places like Toa Baja subject to irreparable damage. This NASA DEVELOP project collaborated with the Municipio Autónomo de Toa Baja, ResilientSEE, and the Massachusetts Institute of Technology Urban Risk Lab to supplement recent 2018 Federal Emergency Management Agency Hydraulic Engineering Centers-River Analysis System flood maps, which designated 63% of the area as a flood plain. The analysis provides a high-resolution interpretation of flood susceptibility using a variety of factors that collectively influence the likelihood of flooding. Sentinel-1 C-Band Synthetic Aperture Radar (C-SAR) data processed with Google Earth Engine scripting identified historical inundation and was used for validation purposes. Socioeconomic factors were combined with the inundation layer producing a final risk output. These outputs will improve public understanding of exposure to flood risk in Toa Baja and provide scientific evidence for flood mitigation advocacy.

Disasters↗

Toa Baja Disasters: Utilizing Open-Source Earth Observations to Inform the Toa Baja Municipality’s Flood Mitigation Efforts and Educate the Public

Toa Baja, located just west of San Juan in Puerto Rico, is known as “the underwater city” due to its propensity to flood. The city contains the mouth of the island’s longest river, Río de la Plata, which drains into the Atlantic Ocean on the northern edge of the municipality. Proximity to these major water features and the flat, low terrain contribute to the flood-prone nature of the area. During tropical storm events, such as Hurricane Maria in 2017, Toa Baja experienced inundation of up to 20 feet. Changes in the global climate system are causing more intense and frequent tropical storms, making places like Toa Baja subject to irreparable damage. This NASA DEVELOP project collaborated with the Municipio Autonómo de Toa Baja, ResilientSEE, and the Massachusetts Institute of Technology Urban Risk Lab to supplement recent 2018 Federal Emergency Management Agency Hydraulic Engineering Centers-River Analysis System flood maps, which designated 63% of the area as a flood plain. The analysis provides a high-resolution interpretation of flood susceptibility using a variety of factors that collectively influence the likelihood of flooding. Sentinel-1 C-Band Synthetic Aperture Radar (C-SAR) data processed with Google Earth Engine scripting identified historical inundation and was used for validation purposes. Socioeconomic factors were combined with the inundation layer producing a final risk output. These outputs will improve public understanding of exposure to flood risk in Toa Baja and provide scientific evidence for flood mitigation advocacy.

Disasters↗

Phyllosilicate Decomposition on Bennu Due to Prolonged Surface Exposure

The Origins, Spectral Interpretation, Resource Identification, and Security–Regolith Explorer (OSIRIS-REx) mission to carbonaceous asteroid (101955) Bennu performed detailed mapping with a suite of instruments to characterize the composition and geology of its surface. Here we use data from the OSIRIS-REx Thermal Emission Spectrometer (OTES) instrument to investigate the relationship of OTES-derived spectral indices to other derived data products from OTES, the OSIRIS-REx Camera Suite (OCAMS), and the OSIRIS-REx Visible and InfraRed Spectrometer (OVIRS) at global and local scales. We quantitatively confirm that high values of the OTES silicate stretching slope (from ∼10 to ∼12 μm) in midday spectra that are indicative of thin and/or patchy dust cover are strongly associated with low thermal inertia (high porosity), low albedo boulders on Bennu. These high porosity boulders have brecciated textures with embedded clasts that likely originated on Bennu's parent body or during its disruption. The high porosity of these boulders is a key factor in the local production of the dust or its entrapment, as some large, brecciated boulders with a lower porosity have little evidence of dust. A second OTES spectral parameter, the silicate bending band depth near 22.7 μm applied to early evening spectra, is not correlated to thermal inertia, but is weakly to strongly correlated to albedo, OVIRS-derived 1.05 μm and 2.74 μm band depths, OVIRS-derived hydrogen abundance, and modeled nanophase troilite abundance. In several regions on Bennu there is a strong spatial relationship between these parameters, whereby areas with shallower silicate bending bands also have shallower 1.05 μm and 2.74 μm bands and lower albedo with higher nanophase troilite abundances. These correlations, combined with analysis of the silicate bending band in laboratory experiments of space weathered and mildly heated carbonaceous chondrites, suggests that decreased silicate bending band depths signify decomposition of phyllosilicates, likely Fe-bearing, due to space weathering or mild heating (<600 °C) via solar radiation during Bennu's time in near-Earth space. There is a strong association of larger silicate bending band depths in areas dominated by small rocks and unresolved material and in areas with small (≤ 25 m) craters identified as the spectrally reddest on Bennu, suggesting that this material has been more recently exposed due to impact and/or mass wasting processes. The shallowest silicate bending depths are associated with larger rocks and boulders that appear to have the longest surface exposure history, although there is band depth variation among them suggesting either initial composition variation that resulted in different responses to space weathering or heating, or varied exposure history of individual boulders themselves. We predict that any grains returned from Bennu with a significant surface exposure history will be characterized by shallower 22.7 μm, 1.05 μm and 2.7 μm band depths and increased sulfide (troilite) abundance, as well as textural and chemical evidence for phyllosilicate dehydration.

Asteroids, surfaces↗

Deployment of a 3D Sonar System at Savannah River: Results, Implications and Next Steps - 19718

Waste solids mapping is required at SRS to support the closure of high level radioactive waste tanks. The current mapping methodologies utilize a combination of video inspections and still camera photos to record the solids location and height in the tank with interpretation by site Engineering personnel. Tank liquid level is recorded during capture of the images to determine the height of solids. If no liquid is present, the images are compared to known waste tank features (e.g., cooling coils, support columns) to determine the height of the solids. In December of 2017, NuVision Engineering and Fortis Engineering conducted a demonstration of Sonar technology in SRS F-Tank Farm (FTF) Tank 7 using three risers (Risers 1, 5, and Center) for the purposes of mapping waste solids in the tank. Tank 7 was not the ideal choice for a 'first-of-a-kind' deployment given its congested internals but was selected based on the priority of tank farm operations. Savannah River Remediation (SRR) evaluated the results of this demonstration against the current solids mapping methodologies to determine potential future application of Sonar technology to support upcoming sludge or salt waste removal, decisions to cease waste removal and perform sampling and analysis, final residuals volume determinations, and applicability for use in H-Tank Farm (HTF) Type IV tanks. The efficiency of the Sonar mapping technology against the current mapping techniques and the relative costs of each were also evaluated. The Sonar demonstration performed in the three risers of Tank 7 resulted in a tank floor coverage of approximately 26%. This limited coverage was primarily due to shadow effects and image distortions created by vertical cooling coils and support columns within the waste tank. A comparison of the images produced during the Sonar demonstration and the historical Tank 7 mapping, performed in 2011 upon completion of Bulk Waste Removal Efforts (BWRE), showed physical similarities between the mounds located near the Sonar deployment risers. The technology performed well in accurately mapping solids in those areas accessible to coverage. The Sonar mapping technology may achieve more benefits if configured to work around internal obstructions, or in a Type IV tank, where the lack of vertical cooling coils may allow for a larger coverage area. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Probing and manipulating magnetic and 2D quantum materials using ultrafast laser and high harmonic sources

The ability to probe the full dynamic response of quantum materials on the length- and time-scales (Å to attoseconds on up) fundamental to charge, spin and phonon interactions is leading to a host of new discoveries. The coupled interactions between charges, spins, orbital and lattice degrees of freedom are key to determining the state of a material, whether metallic, insulating, superconducting or magnetic. Under thermal equilibrium conditions these states can be tuned by varying the temperature, pressure, chemical doping or dimensionality. However, their inherent complexity and rich phase landscape make them challenging to understand or manipulate deterministically. Fortunately, ultrafast light sources have undergone remarkable advances in recent years, achieving what was merely a dream three decades ago, i.e., full coherent control of light fields spanning the THz to the X-ray regions. Here, these new capabilities are providing powerful new tools for coherently manipulating and probing quantum materials using light. Recent advances in high harmonic generation (HHG) have provided new techniques for uncovering new phases and couplings in 2D and magnetic materials, using femtosecond lasers to coherently manipulate and tune the properties and interactions in the material, and HHG as a probe to map out these new phases and couplings. There are several beautiful recent observations of exciting new states in materials using light, but in some cases, the limited experimental data points made it challenging to validate an interpretation. The new ability of ultrafast HHG-based photoemission and magneto-optic spectroscopies to precisely probe multiple phases, electronic and magnetic order parameters, when combined with scanning the laser excitation fluence, is allowing us to map how material phases, magnetic states, and electron-phonon-spin couplings in these delicate materials.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗

A 'digital' technique for manual extraction of data from aerial photography

The interpretation procedure described uses a grid cell approach. In addition, a random point is located in each cell. The procedure required that the cell/point grid be established on a base map, and identical grids be made to precisely match the scale of the photographic frames. The grid is then positioned on the photography by visual alignment to obvious features. Several alignments on one frame are sometimes required to make a precise match of all points to be interpreted. This system inherently corrects for distortions in the photography. Interpretation is then done cell by cell. In order to meet the time constraints, first order interpretation should be maintained. The data is put onto coding forms, along with other appropriate data, if desired. This 'digital' manual interpretation technique has proven to be efficient, and time and cost effective, while meeting strict requirements for data format and accuracy.

Istvan, L. B.↗

A Mass Spectrometry-Based Predictive Strategy Reveals ADAP1 is Phosphorylated at Tyrosine 364

The goal of this work was to identify phosphorylation sites within the amino acid sequence of human ADAP1. Using traditional mass spectrometry-based techniques we were unable to produce interpretable spectra demonstrating modification by phosphorylation. This prompted us to employ a strategy in which phosphorylated peptides were first predicted using peptide mapping followed by targeted MS/MS acquisition. ADAP1 was immunoprecipitated from extracts of HEK293 cells stably-transfected with ADAP1 cDNA. Immunoprecipitated ADAP1 was digested with proteolytic enzymes and analyzed by LC-MS in MS1 mode by high-resolution quadrupole time-of-flight mass spectrometry (QTOF-MS). Peptide molecular features were extracted using an untargeted data mining algorithm. Extracted peptide neutral masses were matched against the ADAP1 amino acid sequence with phosphorylation included as a predicted modification. Peptides with predicted phosphorylation sites were analyzed by targeted LC-MS2. Acquired MS2 spectra were then analyzed using database search engines to confirm phosphorylation. Spectra of phosphorylated peptides were validated by manual interpretation. Further confirmation was performed by manipulating phospho-peptide abundance using calf intestinal phosphatase (CIP) and the phorbol ester, phorbol 12-myristate 13-acetate (PMA). Of five predicted phosphopeptides, one, comprised of the sequence AVDRPMLPQEYAVEAHFK, was confirmed to be phosphorylated on a Tyrosine at position 364. Pre-treatment of cells with PMA prior to immunoprecipitation increased the ratio of phosphorylated to unphosphorylated peptide as determined by area counts of extracted ion chromatograms (EIC). Addition of CIP to immunoprecipitation reactions eliminated the phosphorylated form. A novel phosphorylation site was identified at Tyrosine 364. Phosphorylation at this site is increased by treatment with PMA. PMA promotes membrane translocation and activation of protein kinase C (PKC), indicating that Tyrosine 364 is phosphorylated by a PKC-dependent mechanism.

59 BASIC BIOLOGICAL SCIENCES↗

GIS Integration for Quantitatively Determining the Capabilities of Five Remote Sensors for Resource Exploration

To assist the U.S. Geological Survey in carrying out a Congressional mandate to investigate the use of side-looking airborne radar (SLAR) for resources exploration, a research program was conducted to define the contribution of SLAR imagery to structural geologic mapping and to compare this with contributions from other remote sensing systems. Imagery from two SLAR systems and from three other remote sensing systems was interpreted, and the resulting information was digitized, quantified and intercompared using a computer-assisted geographic information system (GIS). The study area covers approximately 10,000 square miles within the Naval Petroleum Reserve, Alaska, and is situated between the foothills of the Brooks Range and the North Slope. The principal objectives were: (1) to establish quantitatively, the total information contribution of each of the five remote sensing systems to the mapping of structural geology; (2) to determine the amount of information detected in common when the sensors are used in combination; and (3) to determine the amount of unique, incremental information detected by each sensor when used in combination with others. The remote sensor imagery that was investigated included real-aperture and synthetic-aperture radar imagery, standard and digitally enhanced LANDSAT MSS imagery, and aerial photos.

Pascucci, R. F.↗