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At least 811 records · Page 45

Influence of soil heterogeneity on soybean plant development and crop yield evaluated using time-series of UAV and ground-based geophysical imagery

Understanding the interactions among agricultural processes, soil, and plants is necessary for optimizing crop yield and productivity. This study focuses on developing effective monitoring and analysis methodologies that estimate key soil and plant properties. These methodologies include data acquisition and processing approaches that use unmanned aerial vehicles (UAVs) and surface geophysical techniques. In particular, we applied these approaches to a soybean farm in Arkansas to characterize the soil–plant coupled spatial and temporal heterogeneity, as well as to identify key environmental factors that influence plant growth and yield. UAV-based multitemporal acquisition of high-resolution RGB (red–green–blue) imagery and direct measurements were used to monitor plant height and photosynthetic activity. We present an algorithm that efficiently exploits the high-resolution UAV images to estimate plant spatial abundance and plant vigor throughout the growing season. Such plant characterization is extremely important for the identification of anomalous areas, providing easily interpretable information that can be used to guide near-real-time farming decisions. Additionally, high-resolution multitemporal surface geophysical measurements of apparent soil electrical conductivity were used to estimate the spatial heterogeneity of soil texture. By integrating the multiscale multitype soil and plant datasets, we identified the spatiotemporal co-variance between soil properties and plant development and yield. Our novel approach for early season monitoring of plant spatial abundance identified areas of low productivity controlled by soil clay content, while temporal analysis of geophysical data showed the impact of soil moisture and irrigation practice (controlled by topography) on plant dynamics. Our study demonstrates the effective coupling of UAV data products with geophysical data to extract critical information for farm management.

54 ENVIRONMENTAL SCIENCES↗

Analysis of multipacting threshold sensitivity to the random distributions of the secondary electron yield parameters

The way multipacting develops, depends strongly on the secondary emission property of the surface material. The knowledge of secondary electron yield is crucial for accurate prediction of the multipacting threshold. Variations in secondary electron yield parameters from experimental measurements create uncertainty, stemming from handling and surface preparation, and these uncertainties significantly affect multipacting threshold predictions. Despite their significance, the previous studies on the multipacting phenomenon did not adequately address the effect of an assumed random distribution of the secondary emission parameters on the multipacting threshold. Therefore, this paper aims to provide a comprehensive statistical study on how the different random distributions of the secondary emission parameters and, as a result, the uncertainty in the secondary electron yield affect multipacting thresholds. We focus on three commonly used distributions, namely uniform, normal, and truncated normal distributions, to define the uncertainty of random inputs. We use the chaos polynomial expansion method to determine how much each of the random parameters contributes to the multipacting threshold uncertainty. Additionally, we calculate Sobol sensitivity indices to evaluate the impact of the individual parameters or groups of parameters on the model outputs and study how different random distributions of these parameters affected the Sobol index results.

physics↗

Metabolic engineering for the high-yield production of isoprenoid-based C 5 alcohols in E. coli

Branched five carbon (C 5 ) alcohols are attractive targets for microbial production due to their desirable fuel properties and importance as platform chemicals. In this study, we engineered a heterologous isoprenoid pathway in E. coli for the high-yield production of 3-methyl-3-buten-1-ol, 3-methyl-2-buten-1-ol, and 3-methyl-1-butanol, three C 5 alcohols that serve as potential biofuels. We first constructed a pathway for 3-methyl-3-buten-1-ol, where metabolite profiling identified NudB, a promiscuous phosphatase, as a likely pathway bottleneck. We achieved a 60% increase in the yield of 3-methyl-3-buten-1-ol by engineering the Shine-Dalgarno sequence of nudB, which increased protein levels by 9-fold and reduced isopentenyl diphosphate (IPP) accumulation by 4-fold. To further optimize the pathway, we adjusted mevalonate kinase (MK) expression and investigated MK enzymes from alternative microbes such as Methanosarcina mazei. Next, we expressed a fusion protein of IPP isomerase and the phosphatase (Idi1~NudB) along with a reductase (NemA) to diversify production to 3-methyl-2-buten-1-ol and 3-methyl-1-butanol. Lastly, we used an oleyl alcohol overlay to improve alcohol recovery, achieving final titers of 2.23 g/L of 3-methyl-3-buten-1-ol (~70% of pathway-dependent theoretical yield), 150 mg/L of 3-methyl-2-buten-1-ol, and 300 mg/L of 3-methyl-1-butanol.

59 BASIC BIOLOGICAL SCIENCES↗

Surface studies of solids using integral x-ray-induced photoemission yield

X-ray induced photoemission yield contains structural information complementary to that provided by X-ray Fresnel reflectivity, which presents an advantage to a wide variety of surface studies if this information is made easily accessible. Photoemission in materials research is commonly acknowledged as a method with a probing depth limited by the escape depth of the photoelectrons. Here we show that the integral hard-X-ray-induced photoemission yield is modulated by the Fresnel reflectivity of a multilayer structure and carries structural information that extends well beyond the photoelectron escape depth. A simple electric self-detection of the integral photoemission yield and Fourier data analysis permit extraction of thicknesses of individual layers. The approach does not require detection of the reflected radiation and can be considered as a framework for non-invasive evaluation of buried layers with hard X-rays under grazing incidence.

36 MATERIALS SCIENCE↗

Cooperative valorization of lignin and residual sugar to polyhydroxyalkanoate (PHA) for enhanced yield and carbon utilization in biorefineries

Lignin valorization is essential for a sustainable and economically viable biorefinery. Despite the recent efforts, it is still unclear how the reactivity of native lignin can be improved by pretreatment in a biorefinery and how different components in biorefinery residue especially residual sugar contribute to bioconversion in biorefineries. In the present study, co-utilization of lignin and limited glucose was first evaluated and proved to facilitate lignin conversion and polyhydroxyalkanoate (PHA) production. A new integrated biorefinery was then designed to cooperatively valorize lignin and residual sugar for improving the PHA yield and utilization efficiency of biorefinery residue. By the design of integrated biorefinery, the glucose and xylose yield were 91% and 73%, respectively, which increased by 18% and 12% compared with that only using sodium hydroxide pretreatment. Approximately 85% of the lignin was dissolved into a liquid stream with the integrated biorefinery, corresponding to an increase of 69% compared with that only using sodium hydroxide pretreatment. More than 70% of the residual sugar was released from the biorefinery residue, producing the soluble lignin stream that contains both lignin and residual sugar for synergistic bioconversion. Bioconversion of the soluble lignin stream with Pseudomonas putida KT2440 produced 1.5 g l -1 PHA, representing the record titer of PHA from biorefinery residue. The lignin characterization results from nuclear magnetic resonance and gel-permeation chromatography showed that the integrated process significantly reduced the lignin molecular weight, broke down more β-O-4 and β-5 linkages, and enriched the H-lignin content. Alongside the increased residual sugar concentration, the bioconversion performance of soluble lignin to PHA was significantly improved. Altogether, the integrated biorefinery increased the fermentable sugar yield and improved the PHA production from biorefinery waste by cooperative valorization of lignin and residual sugar, which shows potential advantages for biorefinery sustainability.

09 BIOMASS FUELS↗

One-step lignocellulose depolymerization and saccharification to high sugar yield and less condensed isolated lignin

The cost of sugar production remains a key challenge in future lignocellulosic biorefineries. We demonstrate that ZnBr 2 , an inexpensive inorganic salt, provides nearly theoretical yields of glucose and xylose in one-step from poplar wood at 85 °C and short reaction times at molten salt hydrate (MSH) conditions without an acid. Catalytic depolymerization of the isolated MSH lignin, using a CoS 2 catalyst, yields 17% phenol-like monomers compared to only 1% produced from the acidified MSH lignin. Reductive catalytic fractionation of MSH lignin over Ru/C resulted in two times higher total monomer yield compared to the AMSH lignin. Both the lignin samples were characterized using 2D HSQC NMR and the thioacidolysis method. Thioacidolysis studies reveal 8.4% and 1.8% of β-O-4 linkages in MSH and acidified MSH lignin, respectively. Thermodynamic modeling and 13 C NMR spectroscopy indicate that the effectiveness of this catalyst arises from the strong interaction of the Lewis acidic zinc cation (Zn 2+ ) with the coordinated water molecules resulting in hydrolysis of the metal aquo complex and to the salt-driven increase in the H + activity coefficient. Techno-economic analysis demonstrates that despite being slower, the ZnBr 2 MSH media has cost advantages, compared to conventional hydrolysis and even to the LiBr and ZnBr 2 AMSH, due to the higher quality of lignin.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Conversion of cellulose to high-yield glucose in water over sulfonated mesoporous carbon fibers with optimized acidity

The hydrolysis of cellulose to high-yield glucose in water remains challenging owing to the limited acidity and substrate contact of sulfonated carbons. In this study, we report a unique sulfonated mesoporous carbon fiber with optimized acidity prepared by the pyrolysis of FeCl 3 -impregnated wipe fibers followed by acid treatment. The Bronsted acidity (especially for SO 3 H) was produced by sulfonation of the magnetic carbon fiber intermediate, while Lewis acidity originated from small Fe 3 O 4 nanoparticles and the remaining defects. The balanced Bronsted and Lewis acidity combined with hydrophilic functionalities was simultaneously tailored by varying the Fe/wipe fiber (WF) feed ratio and pyrolysis temperature to change the porous structure of the final catalysts. The elaborately fabricated MCF-SO 3 H-20-500 (20% Fe/WF feed ratio and pyrolysis at 500 degrees C) delivered 66.0% glucose yield as cellulose was completely converted. This is the highest glucose yield achieved by the hydrolysis of untreated cellulose in water over a sulfonated carbon catalyst. The optimized Bronsted and Lewis acidity was mainly responsible for the controlled conversion of cellulose to glucose, while the rich hydrophilic functionalities and superior mesoporous structure of the catalyst facilitated contact to cellulose and mass transfer. This investigation will open new avenues to design high-performance carbon sulfoacid catalysts toward cellulose hydrolysis.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Protection and enrichment: how two different carbonaceous biofilm supports improve methane yield from encapsulated anaerobic microorganisms

Encapsulating anaerobic microorganisms allows for the separation of the solids retention time from the hydraulic retention time during anaerobic wastewater treatment. The harsh chemistries involved in the process of encapsulation can have adverse effects on microorganisms for anaerobic digestion, especially methanogens, and can lead to lower methane yields after encapsulation. Improving the survival and maintaining activity of anaerobic communities during encapsulation will likely be the key to improving methane yield. In this study, we investigated the encapsulation of biomass grown as biofilms on two carbonaceous materials, biochar and powdered activated carbon (PAC), to improve methane yield. Microorganisms grown as biofilms on biochar and PAC were encapsulated in polyethylene glycol (PEG) and incubated for 10 days. After 10 days, the unamended control capsules produced 81.6 ± 5.4 μmol of methane, while PAC-amended capsules produced 129.8 ± 1.9 μmol and biochar-amended capsules produced 432.96 ± 20.8 μmol methane, with the differences being statistically significant (p < 0.05). In biochar, a higher relative abundance of methanogens led to increased methane production capacity. The ratio of the methyl coenzyme M reductase (mcrA) genes to total 16S rRNA genes in the encapsulated biochar-supported biofilms was significantly higher than that in the encapsulated unsupported (p = 4.9 × 10 −5 ) and the PAC-supported biofilms (p = 0.012). Biochar-supported biofilms also had higher methane output per mcrA or 16S rRNA gene copy number. For the PAC, biofilms were protected from ammonium persulfate (APS), a powerful oxidant used in the encapsulation process. PAC removed 92% of dissolved APS, reducing exposure of the methanogens to this chemical. Unfortunately, this removal of APS compromised capsule stability, limiting the amount of PAC that could be added to the capsules. Furthermore, amendments that improve survival and activity of methanogens should be used in the capsules instead of those that protect methanogens by interfering with encapsulant polymerization chemistry.

Resource recovery↗

Sustainable strategies to achieve industrial ethanol titers from different bioenergy feedstocks: scale-up approach for better ethanol yield

Hydrothermal pretreatment is a promising approach to lignocellulosic biomass processing for enzymatic hydrolysis and high-yield bioethanol fermentation, as it reduces downstream inhibitor content and the amount of toxic byproducts generated. In this paper, the ethanol yield and productivity of an engineered xylose-fermenting strain of Saccharomyces cerevisiae were tested on lignocellulosic hydrolysates produced with varying citrate buffer concentration, solid loading, supplemental nitrogen source, and feedstock of origin, and a semi-integrated bioprocess which integrates enzymatic hydrolysis and bioethanol fermentation was developed. The greatest ethanol yields (g p /g s ) of 0.490 ± 0.008, 0.460 ± 0.001, 0.420 ± 0.002 and 0.410 ± 0.002 were obtained from bioenergy sorghum (BES), Miscanthus × giganteus (MG), energy cane (EC), and oilcane (OC), respectively. In addition, an equivalent of 291 L, 253.54 L, 257.8 L, and 260.3 L of bioethanol were produced per ton of BES, MG, EC, and OC, respectively, by using urea as a nitrogen source in a bioreactor.

09 BIOMASS FUELS↗

Energy dependence of fission product yields from 235 U, 238 U, and 239 Pu with monoenergetic neutrons between thermal and 14.8 MeV

Under a joint collaboration between TUNL-LANL-LLNL, a set of absolute fission product yield measurements has been performed. The energy dependence of a number of cumulative fission product yields (FPY) have been measured using quasi-monoenergetic neutron beams for three actinide targets, 235 U, 238 U and 239 Pu, between 0.5 and 14.8 MeV. The FPYs were measured by a combination of fission counting using specially designed dual-fission chambers and γ-ray counting. Each dual-fission chamber is a back-to-back ionization chamber encasing an activation target in the center with thin deposits of the same target isotope in each chamber. This method allows for the direct measurement of the total number of fissions in the activation target with no reference to the fission cross-section, thus reducing uncertainties. γ-ray counting of the activation target was performed on well-shielded HPGe detectors over a period of two months post irradiation to properly identify fission products. Reported are absolute cumulative fission product yields for incident neutron energies of 0.5, 1.37, 2.4, 3.6, 4.6, 5.5, 7.5, 8.9 and 14.8 MeV. Preliminary results from thermal irradiations at the MIT research reactor will also be presented and compared to present data and evaluations.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗

Precision measurements of K{sup {+-}} {yields} {pi}{sup {+-}}{pi}{sup 0}{gamma} decay at NA48/2 and search for CP violation

The direct emission (DE) and interference (INT) terms of the radiative decay K{sup {+-}} {yields} {pi}{sup {+-}}{pi}{sup 0}{gamma} have been measured by the NA48/2 experiment at CERN SPS. About 6 10{sup 5} K{sup {+-}} {yields} {pi}{sup {+-}}{pi}{sup 0}{gamma} decays have been selected from data collected during 2003 and 2004. The relative amounts of DE and INT with respect to the dominant Inner Bremsstrahlung (IB) contribution have been measured in the range 0<T{sub {pi}}{sup *}<80 MeV, where T{sub {pi}}{sup *} is the kinetic energy of the charged pion in the kaon rest frame, obtaining: Frac(DE) = (3.32 {+-}0.15{sub stat}{+-}0.14{sub syst})10{sup -2}, Frac(INT) = (-2.35 {+-}0.35{sub stat}{+-}0.39{sub syst}) 10{sup -2}. This is the first observation of an interference term in K{sup {+-}} {yields} {pi}{sup {+-}}{pi}{sup 0}{gamma} decays. From this measurement the DE normalized electric and magnetic amplitudes are derived: X{sub E} = (-24 {+-}6) GeV{sup -4}, X{sub M} = (254 {+-} 9) GeV{sup -4}.In addition, the CP violating asymmetry in the K{sup +} and K- branching ratios for this channel has been determined to be less than 1.5 10{sup -3} at 90% confidence level.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Origin of 2.7 {mu}m luminescence and energy transfer process of Er{sup 3+}: {sup 4}I{sub 11/2}{yields}{sup 4}I{sub 13/2} transition in Er{sup 3+}/Yb{sup 3+} doped germanate glasses

An investigation of spectroscopic property and energy transfer process of Er{sup 3+} doped and Er{sup 3+}/Yb{sup 3+} co-doped germanate glasses is presented. The emission cross section of the {sup 4}I{sub 11/2}{yields}{sup 4}I{sub 13/2} transition of Er{sup 3+} doped germanate glass is calculated to be 1.2 x 10{sup -20} cm{sup 2}. The microparameter of energy transfer from Yb{sup 3+}:{sup 2} F{sub 5/2} to Er{sup 3+}:{sup 4}I{sub 11/2} is calculated to be 2.74 x 10{sup -39} cm{sup 6}/s, and that is not phonon dependent in the quasiresonant process. The intensity of the emission around 2.7 {mu}m is enhanced obviously when Er{sup 3+} co-doped with Yb{sup 3+}. The excited-state relaxation process of Er{sup 3+} is adequately described by a combination of the Judd-Ofelt model and the energy-gap law. With the exception of {sup 4}I{sub 13/2} and {sup 4}I{sub 11/2} levels, multiphonon relaxation is dominant for all excited states, making it possible to efficiently pump the 1.55 {mu}m {sup 4}I{sub 13/2}{yields}{sup 4}I{sub 15/2} and 2.7 {mu}m {sup 4}I{sub 13/2}{yields}{sup 4}I{sub 11/2} emission by excitation of Yb{sup 3+}:{sup 2}F{sub 5/2} and Er{sup 3+}:{sup 4}I{sub 11/2} at 980 nm.

36 MATERIALS SCIENCE↗

Copper activation deuterium-tritium neutron yield measurements at the National Ignition Facility

A DT neutron yield diagnostic based on the reactions, {sup 63}Cu(n,2n){sup 62}Cu({beta}{sup +}) and {sup 65}Cu(n,2n) {sup 64} Cu({beta}{sup +}), has been fielded at the National Ignition Facility (NIF). The induced copper activity is measured using a NaI {gamma}-{gamma} coincidence system. Uncertainties in the 14-MeV DT yield measurements are on the order of 7% to 8%. In addition to measuring yield, the ratio of activities induced in two, well-separated copper samples are used to measure the relative anisotropy of the fuel {rho}R to uncertainties as low as 5%.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Soft contribution to B{yields}{gamma} Script-Small-L {nu}{sub Script-Small-L} from dispersion relations and duality

The decay B{yields}{gamma} Script-Small-L {nu}{sub Script-Small-L} receives a numerically large soft-overlap contribution which is formally next-to-leading order in the expansion in the inverse photon energy, 1/E{sub {gamma}}. We point out that this contribution can be calculated with little model-dependence making use of dispersion relations and duality, in full analogy with the similar contribution to the {gamma}{gamma}*{yields}{pi} transition form factor. This result strengthens the case for the utility of the B{yields}{gamma} Script-Small-L {nu}{sub Script-Small-L} decay for constraining the B-meson distribution amplitude and determining its most important parameter, the inverse moment {lambda}{sub B}.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Plasmonic light yield enhancement of a liquid scintillator

We demonstrate modifications to the light yield properties of an organic liquid scintillator due to the localization of the tertiary fluorophore component to the surface of Ag-core silica-shell nanoparticles. We attribute this enhancement to the near-field interaction of Ag nanoparticle plasmons with these fluor molecules. The scintillation light yield enhancement is shown to be equal to the fluorescence enhancement within measurement uncertainties. With a suitable choice of plasmon energy and scintillation fluor, this effect may be used to engineer scintillators with enhanced light yields for radiation detection applications.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Measurement of neutron spectra generated from bombardment of 4 to 24 MeV protons on a thick {sup 9}Be target and estimation of neutron yields

A systematic study on the measurement of neutron spectra emitted from the interaction of protons of various energies with a thick beryllium target has been carried out. The measurements were carried out in the forward direction (at 0° with respect to the direction of protons) using CR-39 detectors. The doses were estimated using the in-house image analyzing program autoTRAK-n, which works on the principle of luminosity variation in and around the track boundaries. A total of six different proton energies starting from 4 MeV to 24 MeV with an energy gap of 4 MeV were chosen for the study of the neutron yields and the estimation of doses. Nearly, 92% of the recoil tracks developed after chemical etching were circular in nature, but the size distributions of the recoil tracks were not found to be linearly dependent on the projectile energy. The neutron yield and dose values were found to be increasing linearly with increasing projectile energies. The response of CR-39 detector was also investigated at different beam currents at two different proton energies. A linear increase of neutron yield with beam current was observed.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

The effect of mix on capsule yields as a function of shell thickness and gas fill

An investigation of direct drive capsules with different shell thicknesses and gas fills was conducted to examine the amount of shock induced (Richtmyer-Meshkov) mix versus Rayleigh-Taylor mix from deceleration of the implosion. The RAGE (Eulerian) code with a turbulent mix model was used to model these capsules for neutron yields along with time-dependent mix amounts. The amount of Richtmyer-Meshkov induced mix from the shock breaking out of the shell is about 0.1 μg (0.15 μm of shell material), while the Rayleigh-Taylor mix is of order 1 μg and determines the mixed simulation yield. The simulations were able to calculate a yield over mix (YOM) ratio (experiment/mix simulation) between 0.5 and 1.0 for capsules with shell thicknesses ranging from 7.5 to 20 μm and with gas fills between 3.8 and 20 atm of D{sub 2} or DT. The simulated burn averaged T{sub ion} values typically lie with 0.5 keV of the data, which is within the measurement error. For capsules with shell thicknesses >25 μm, the YOM values drop to 0.10 ± 0.05, suggesting that some unmodeled effect needs to be accounted for in the thickest capsules.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Determination of relative krypton fission product yields from 14 MeV neutron induced fission of 238 U at the National Ignition Facility

Precisely-known fission yield distributions are needed to determine a fissioning isotope and the incident neutron energy in nuclear security applications. 14 MeV neutrons from DT fusion at the National Ignition Facility (NIF) induce fission in depleted uranium (DU) contained in the target assembly hohlraum. The fission yields of Kr isotopes (85m, 87, 88, and 89) are measured relative to the cumulative yield of 88Kr and compared to previously tabulated values. Here, the results from this experiment and England and Rider are in agreement, except for the 85m Kr/ 88 Kr ratio, which may be the result of incorrect nuclear data.

22 GENERAL STUDIES OF NUCLEAR REACTORS↗