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At least 811 records · Page 45

Intermediate conformation between native β-sheet and non-native α-helix is a precursor of trifluoroethanol-induced aggregation of Human Carbonic Anhydrase-II

Highlights: • HCAII forms amyloid-like aggregates at moderate concentration of trifluoroethanol. • Protein adopts a state between β-sheet and α-helix at moderate % of TFE. • Hydrophobic surface(s) of partially structured conformation forms amyloid. • High % of TFE induces stable α-helical state preventing aggregation. - Abstract: In the present work, we examined the correlation between 2,2,2-trifluoroethanol (TFE)-induced conformational transitions of human carbonic anhydrase II (HCAII) and its aggregation propensity. Circular dichroism data indicates that protein undergoes a transition from β-sheet to α-helix on addition of TFE. The protein was found to aggregate maximally at moderate concentration of TFE at which it exists somewhere between β-sheet and α-helix, probably in extended non-native β-sheet conformation. Thioflavin-T (ThT) and Congo-Red (CR) assays along with fluorescence microscopy and transmission electron microscopy (TEM) data suggest that the protein aggregates induced by TFE possess amyloid-like features. Anilino-8-naphthalene sulfonate (ANS) binding studies reveal that the exposure of hydrophobic surface(s) was maximum in intermediate conformation. Our study suggests that the exposed hydrophobic surface and/or the disruption of the structural features protecting a β-sheet protein might be the major reason(s) for the high aggregation propensity of non-native intermediate conformation of HCAII.

60 APPLIED LIFE SCIENCES↗

Anomalous snow accumulation over the southeast region of the Greenland ice sheet during 2002-2003 snow season

Our objective is to determine seasonal snow accumulation in the percolation zone of the Greenland ice sheet on the daily-weekly basis over the large scale. Our approach utilizes data from the Greenland Climate Network (GC-Net) and from the SeaWinds Scatterometer on the QuikSCAT satellite (QSCAT) to measure snow accumulation (SA) in the percolation zone of the Greenland ice sheet. GC-Net measurements provide crucial in-situ data to facilitate the interpretation of QSCAT backscatter signature for the development of an algorithm to map SA.

Greenland↗

Evaluation of Droplet Splashing Algorithm in LEWICE 3.0

The Icing Branch at NASA Glenn Research has developed a computer program to simulate ice formation on the leading edge of an aircraft wing during flight through cold, moist air. As part of the branch's current research, members have developed software known as LEWICE. This program is capable of predicting the formation of ice under designated weather conditions. The success of LEWICE is an asset to airplane manufacturers, ice protection system manufacturers, and the airline industry. Simulations of ice formation conducted in the tunnel and in flight is costly and time consuming. However, the danger of in-flight icing continues to be a concern for both commercial and military pilots. The LEWICE software is a step towards inexpensive and time efficient prediction of ice collection. In the most recent version of the program, LEWICE contains an algorithm for droplet splashing. Droplet splashing is a natural occurrence that causes accumulation of ice on aircraft surfaces. At impingement water droplets lose a portion of their mass to splashing. With part of each droplet joining the airflow and failing to freeze, early versions of LEWICE without the splashing algorithm over-predicted the collection of ice on the leading edge. The objective of my project was to determine whether the revised version of LEWICE accurately reflected the ice collection data obtained from the Icing Research Tunnel (IRT). The experimental data from the IRT was collected by Mark Potapczuk in January, March and July of 2001 and April and December of 2002. Experimental data points were the result of ice tracings conducted shortly after testing in the tunnel. Run sheets, which included a record of velocity, temperature, liquid water content and droplet diameter, served as the input of the LEWICE computer program. Parameters identical to the tunnel conditions were used to run LEWICE 2.0 and LEWICE 3.0. The results from IRT and versions of LEWICE were compared graphically. After entering the raw experimental data and computer output into a spread sheet, I mapped each ice formation onto a clean airfoil. The LEWICE output provided the data points to graphically depict ice formations developed by the program. weather conditions of runs conducted in January 2001, it was evident that the splashing algorithm of LEWICE 3.0 predicts ice formations more accurately than LEWICE 2.0. Especially at conditions with droplet size between 80 and 160 microns, the splashing algorithm of the new LEWICE version compensated for the loss of droplet mass as a result of splashing. In contrast, LEWICE 2.0 consistently over-predicted the mass of the ice in conditions with droplet size exceeding 80 microns. This evidence confirms that changes made to algorithms of LEWICE 3.0 have increased the accuracy of predicting ice collection.

Homenko, Hilary N.↗

The GLAS Standard Data Products Specification-Level 1, Version 9

The Geoscience Laser Altimeter System (GLAS) is the primary instrument for the ICESat (Ice, Cloud and Land Elevation Satellite) laser altimetry mission. ICESat was the benchmark Earth Observing System (EOS) mission for measuring ice sheet mass balance, cloud and aerosol heights, as well as land topography and vegetation characteristics. From 2003 to 2009, the ICESat mission provided multi-year elevation data needed to determine ice sheet mass balance as well as cloud property information, especially for stratospheric clouds common over polar areas. It also provided topography and vegetation data around the globe, in addition to the polar-specific coverage over the Greenland and Antarctic ice sheets.This document defines the Level-1 GLAS standard data products. This document addresses the data flow, interfaces, record and data formats associated with the GLAS Level 1 standard data products. GLAS Level 1 standard data products are composed of Level 1A and Level 1B data products. The term standard data products refers to those EOS instrument data that are routinely generated for public distribution. The National Snow and Ice Data Center (NSDIC) distribute these products. Each data product has a unique Product Identification code assigned by the Senior Project Scientist. GLAS Level 1A and Level 1B Data Products are composed from those Level 0 data that have been reformatted or transformed to corrected and calibrated data in physical units at the full instrument rate and resolution.

Lee, Jeffrey E.↗

Detailed observations of the plasma sheet during a substorm on April 24, 1979

Plasma, magnetic field, and energetic particle data obtained by ISEE 1 and 2 satellites for the April 24, 1979 substorm are studied in relation to the neutral line model and the boundary layer model. The ISEE 1 and 2 instruments and experiments utilized to collect the data are discussed. The major reconfiguration of the tail plasma and magnetic field plasma region, and the plasma ion flows observed support the neutral line model for interpreting substorms. The plasma ion distribution function and plasma flow are examined. The region of lobe-plasma sheet interface referred to as the separatrix layer is identified. The differences in times of plasma sheet dropout and recovery, and absence or presence of flux anisotropies are investigated. Energetic particle measurements are analyzed to study the relationship between energetic ions and plasma ions, and the velocity distributions. The data support the application of the neutral line model to the evaluation of substorms; however, the data are inconsistent with the boundary layer dynamics model.

Hones, E. W., Jr.↗

Central Plasma Sheet Ion Properties as Inferred from Ionospheric Observations

A method of inferring central plasma sheet (CPS) temperature, density, and pressure from ionospheric observations is developed. The advantage of this method over in situ measurements is that the CPS can be studied in its entirely, rather than only in fragments. As a result, for the first time, comprehensive two-dimensional equatorial maps of CPS pressure, density, and temperature within the isotropic plasma sheet are produced. These particle properties are calculated from data taken by the Special Sensor for Precipitating Particles, version 4 (SSJ4) particle instruments onboard DMSP F8, F9, F10, and F11 satellites during the entire year of 1992. Ion spectra occurring in conjunction with electron acceleration events are specifically excluded. Because of the variability of magnetotail stretching, the mapping to the plasma sheet is done using a modified Tsyganenko [1989] magnetic field model (T89) adjusted to agree with the actual magnetotail stretch at observation time. The latter is inferred with a high degree of accuracy (correlation coefficient -0.9) from the latitude of the DMSP b2i boundary (equivalent to the ion isotropy boundary). The results show that temperature, pressure, and density all exhibit dawn-dusk asymmetries unresolved with previous measurements. The ion temperature peaks near the midnight meridian. This peak, which has been associated with bursty bulk flow events, widens in the Y direction with increased activity. The temperature is higher at dusk than at dawn, and this asymmetry increases with decreasing distance from the Earth. In contrast, the density is higher at dawn than at dusk, and there appears to be a density enhancement in the low-latitude boundary layer regions which increases with decreasing magnetic activity. In the near-Earth regions, the pressure is higher at dusk than at dawn, but this asymmetry weakens with increasing distance from the Earth and may even reverse so that at distances X less than approx. 10 to -12 R(sub E), depending on magnetic activity, the dawn sector has slightly higher pressure. The temperature and density asymmetries in the near-Earth region are consistent with the ion westward gradient/curvature drift as the ions ExB convect earthward. When the solar wind dynamic pressure increases, CPS density and pressure appear to increase, but the temperature remains relatively constant. Comparison with previously published work indicates good agreement between the inferred pressure, temperature, and density and those obtained from in situ data. This new method should provide a continuous mechanism to monitor the pressure, temperature, and density in the magnetotail with unprecedented comprehensiveness.

Wing, Simon↗

Trends in Antarctic Surface Temperatures and Ice Extent from Satellite and Surface Observations

Surface air temperatures observed from stations around Antarctica have indicated predominantly positive trends which are as high as 0.4K per decade along the Antarctic Peninsula. Since the average air temperature during summer in the West Antarctic region is about -6 K, and since most of the stations are near coastal regions, it is important to know whether such trends are due to a local or large scale phenomenon. We analyzed about 19 years of infrared satellite data (1979-1997) to obtain a spatially detailed study of the variability of surface temperatures over the entire Antarctic region. We also analyzed potential impact of temperature changes over the sea ice cover using co-registered and nearly coincident microwave satellite data. The surface temperatures inferred from infrared data are shown to agree well with those observed from the limited number of Antarctic station data with a correlation coefficient of 0.98 and a standard deviation of less than 3K. The 19-year temperature data set shows positive trends of 0.12, 0.68, and 0.41 C per decade in ocean, sea ice and ice sheet surfaces, respectively. The sensitivity of the trend to record length was studied, using long term station data sets, and the results show that the trends start to stabilize at around 2 decades which is about the record length of satellite data. However, interannual fluctuations of the temperatures are large, especially in the ice sheets, and the 95% confidence level for the trends ranges from 0.12 to 1.22 for sea ice and from -0.74 to 1.55 C per decade for the ice sheet. In the sea ice regions, the position of the ice edge in winter with respect to the northernmost position of sea ice during the study period is also shown to be influenced by alternating warm and cold anomalies around the continent. This pattern is similar to that suggested by the presence of the Antarctic Circumpolar Wave (ACW) but the wavenumber appears to be predominantly mode 3 (instead of reported mode 2) for the Antarctic region. Unexpectedly, trends in the sea ice extent are found to be positive in most Antarctic sectors during the same period with the exception of the Bellingshausen and Amundsen Seas region.

Comisc, J. C.↗

Rapid Prototyping Of Layered Composite Parts

Numerically controlled cutting accelerates fabrication of layers. Proposed method derived from stereoscopic lithography. CATIA or CAEDS computer program used to generate three-dimensional mathematical model of prototype part. In model, geometry of part specified in layers, as in stereoscopic lithography. Model data for each layer fed to computer-numerically-controlled ultrasonic cutting machine. Sheet of prepreg (uncured composite material) of specified layer thickness placed in machine and cut, under control of model data, to specified shape of layer.

Wolff, Edwin D.↗

Impact and volcanism - A momentum scaling law for erosion

The controversy that raged through the 1950s to 1970s over similarities and differences between meteorite impact and volcanic processes is revisited. We propose that there are quantitative similarities in erosion caused by high-speed ejecta produced by either impacts or volcanic processes. Field and petrographic data from the Manicouagan impact crater, Canada, are used to demonstrate that, during the emplacement of the impact melt sheet, erosion occurred at a rate of 2562 kg/sq m per s. Field data for the Mount St. Helens lateral blast of May 18, 1980, suggest an erosion rate of 21 kg/sq m per s, and field data for a small pyroclastic flow on August 7, 1980, suggest an erosion rate of about 14 kg /sq m per s. It is proposed that these three rates were determined dominantly by the momentum of the ejecta, and a quantitative formulation based on lofting theory is given. A new application of the Monte Carlo approach to analysis provides minimum, most likely, and maximum estimates for both the field and the theoretical analyses. The substantial erosion that occurs by fast moving flows results in mixing of stratigraphic components over large distances and to very fine scales.

Simonds, Charles H.↗

Simulations of Firn Processes Over the Greenland and Antarctic Ice Sheets: 1980-2021

Conversion of altimetry-derived ice-sheet volume change to mass requires an understanding of the evolution of the combined ice and air content within the firn column. In the absence of suitable techniques to observe the changes to the firn column across the entirety of an ice sheet, the firn column processes are typically modeled. Here, we present new simulations of firn processes over the Greenland and Antarctic ice sheets (GrIS and AIS) using the Community Firn Model and atmospheric reanalysis variables for more than four decades. A data set of more than 250 measured depth–density profiles from both ice sheets provides the basis of the calibration of the dry-snow densification scheme. The resulting scheme results in a reduction in the rate of densification, relative to a commonly used semi-empirical model, through a decreased dependence on the accumulation rate, a proxy for overburden stress. The 1980–2020 modeled firn column runoff, when combined with atmospheric variables from MERRA-2, generates realistic mean integrated surface mass balance values for the Greenland (+390 Gt yr(exp −1)) and Antarctic (+2612 Gt yr(exp −1)) ice sheets when compared to published model-ensemble means. We find that seasonal volume changes associated with firn air content are on average approximately 2.5 times larger than those associated with mass fluxes from surface processes for the AIS and 1.5 times larger for the GrIS; however, when averaged over multiple years, ice and air-volume fluctuations within the firn column are of comparable magnitudes. Between 1996 and 2019, the Greenland Ice Sheet lost nearly 5% of its firn air content, indicating a reduction in the total meltwater retention capability. Nearly all (94 %) of the meltwater produced over the Antarctic Ice Sheet is retained within the firn column through infiltration and refreezing.

Greenland↗

Theory and simulations of broadband electrostatic noise in the geomagnetic tail

The excitation mechanism for broadband electrostatic noise (BEN) and the effects of BEN particles in the geomagnetic tail are examined using the linear analysis theory and particle simulations. The linear theory for electrostatic instabilities is discussed. The plasma sheet particle population is simulated using counter-streaming cold ion beams, and warm ions and electrons. The ion-ion instability, ion-acoustic mode, and the electrostatic ion cyclotron harmonic waves are studied. The velocity distributions, electric field intensity, and electron plasma waves for the plasma sheet boundary layer are evaluated. The frequency wave spectrum and particle distributions are computed and analyzed. The conditions for the two simulations, which differ only in beam ion drift speed, are described; it is observed that in the first simulation the dominate modes propagate parallel to the magnetic field and in the second simulation the propagation modes are oblique. The simulation data reveal that when beam temperature is smaller than plasma sheet temperature ion-acoustic and ion-ion instabilities grow to large amplitudes heating both electrons and ions. The data are compared to ISEE-1 observations and good correlation is obtained.

Ashour-Abdalla, M.↗

Thin Current Sheets of Sub-Ion Scales Observed by MAVEN in the Martian Magnetotail

Current sheets (CSs) play a crucial role in the storage and conversion of magnetic energy in planetary magnetotails. Using high‐resolution magnetic field data from MAVEN spacecraft, we report the existence of super thin current sheets (STCSs) in the Martian magnetotail. The typical half‐thickness of the STCSs is ~5 km, and it is much less than the gyroradius of thermal protons (ρp). The STCSs are embedded into a thicker sheet with L ≥ ρp forming a multiscale current configuration. The formation of STCS does not depend on ion composition, but it is controlled by the small value of the normal component of the magnetic field at the neutral plane (BN). A number of the observed multiscale CSs are located in the parametric map close to the tearing‐unstable domain, and thus, the inner STCS can provide an additional free energy to excite ion tearing mode in the Martian magnetotail.

Martian↗

Light echoes and transient luminescence near SN 1987A

The discovery of two new light echoes from sheets of material behind supernova 1987A and present images of the progenitor's circumstellar shell are reported, indicating diffuse echoes from the star's red giant wind. The echo sheets' geometry explains well the behavior of SN 1987's 10-micron flux, but the circumstellar shell appears to be 70 percent larger than the prediction from the analysis of narrow UV emission lines. The sheets' recombination time show them relatively thin and dense. The data also constrain the existence of any fourth star in the Sanduleak -69 deg 202 system and show that the feature reported 8 arcsecs from the supernova is probably not an echo from a thin sheet in SN 1987A's foreground.

Crotts, Arlin P. S.↗

Analysis of Imp-C data from the magnetospheric tail

Satellite magnetic field measurements in the geomagnetic tail current sheet are analyzed to determine the normal field component, and other CS parameters such as thickness, motion, vector current density, etc., and to make correlations with auroral activity as measured by the A sub e index. The satellite data used in the initial part of this study were from Explorer 28 and Explorer 34 satellites.

Speiser, T. W.↗

Mineralogical diversity (spectral reflectance and Moessbauer data) in compositionally similar impact melt rocks from Manicouagan Crater, Canada

Meteoritic impacts under oxidizing surface conditions occur on both earth and Mars. Oxidative alteration of impact melt sheets is reported at several terrestrial impact structures including Manicouagan, West Clearwater Lake, and the Ries Basin. A number of studies have advocated that a significant fraction of Martian soil may consist of erosional products of oxidatively altered impact melt sheets. If so, the signature of the Fe-bearing mineralogies formed by the process may be present in visible and near infrared reflectivity data for the Martian surface. Of concern is what mineral assemblages form in impact melt sheets produced under oxidizing conditions and what their spectral signatures are. Spectral and Moessbauer data for 19 powder samples of impact melt rock from Manicouagan Crater are reported. Results show for naturally occurring materials that composite hematite-pyroxene bands have minima in the 910-nm region. Thus many of the anomalous Phobos-2 spectra, characterized by a shallow band minimum in the near-IR whose position varies between approximately 850 and 1000 nm, can be explained by assemblages whose endmembers (hematite and pyroxene) are accepted to be present on Mars. Furthermore, results show that a mineralogically diverse suite of rocks can be generated at essentially constant composition, which implies that variations in Martian surface mineralogy do not necessarily imply variations in chemical composition.

Morris, R. V.↗

The Jovian magnetotail and its current sheet

Analyses of Voyager magnetic field measurements have extended the understanding of the structural and temporal characteristics of Jupiter's magnetic tail. The magnitude of the magnetic field in the lobes of the tail is found to decrease with Jovicentric distance approximately as r to he-1.4, compared with the power law exponent of -1.7 found for the rate of decrease along the Pioneer 10 outbound trajectory. Voyager observations of magnetic field component variations with Jovicentric distance in the tail do not support the uniform radial plasma outflow model derived from Pioneer data. Voyager 2 has shown that the azimuthal current sheet which surrounds Jupiter in the inner and middle magnetosphere extends tailward (in the anti-Sun direction) to a distance of at least 100 R sub J. In the tail this current sheet consists of a plasma sheet and embedded neutral sheet. In the region of the tail where the sheet is observed, the variation of the magnetic field as a result of the sheet structure and its 10 hr periodic motion is the dominant variation seen.

Behannon, K. W.↗