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At least 847 records · Page 47
All sourcesNASA NTRSDOE OSTI
NASA NTRSDate not supplied

Flow Characterization of the NASA Langley Unitary Plan WindTunnel, Test Section 2: Computational Results

Flow in the empty Unitary Planform Wind Tunnel at the NASA Langley Research Center issimulated with computational fluid dynamics methods. The objectives are to assess CFD’s truepredictive capability and to generate flow-field maps upstream of the tunnel’s test section foruse in associated vehicle tests. Multiple CFD solvers, grid adaption methods, and turbulencemodels are used by five teams doing the simulations. The simulation domain is as large aspractical: from the start of the settling chamber, just downstream of the last set of turningvanes, to well downstream of the test section. Simulations are done at three Mach numbers andseveral Reynolds numbers that cover most of the tunnel’s operating range. The sensitivity ofthe CFD solutions to simulation process details, including turbulence model, spatial resolutionand settling-chamber inflow velocity profile, is characterized. Significant sensitivities in somedetails of the predicted test section flow are described. CFD predicts that details of flow inthe settling chamber can affect the flow in the test section. Turning of the flow through thetunnel contraction and past nozzle-block hardware generates streamwise vortices, some ofwhich survive into the core of the test section, principally at low Mach number. Preliminarycomparisons to experimental measurements are given. The major flow characteristics andthe dynamical vortices predicted by CFD exist in the experiment. Several details in CFD andexperiment are observed to differ, including the effects of the vortices and features that appearto be Mach waves in the test section. Sensitivity to grid resolution and turbulence modelingis noted in the vortices; no potential cause for the differences in Mach waves has yet beenidentified in the CFD sensitivity studies.

wind tunnel↗
DOE OSTI2018

Wavelet multiresolution analysis of particle-laden turbulence

Direct numerical simulations of incompressible homogeneous-isotropic turbulence laden with a dilute suspension of inertial point particles are performed in conjunction with a wavelet multiresolution analysis of the results. The use of spatially localized wavelet basis functions enables the simultaneous consideration of physical and scale spaces in the spectral characterization of the flow field of the carrier phase and the concentration field of the dispersed phase. The multiresolution analysis of the dispersed phase provides statistical information about the spatial variabilities of a scale-dependent coarse-grained number density field and the local energy spectra of its fluctuations, characterizing the sensitivities of those quantities to variations in scale and Stokes number. In particular, the spatial variabilities of the wavelet energy spectrum of the particle concentration fluctuations are observed to be maximum in regimes where the particles preferentially concentrate. The results highlight the scale-dependent inhomogeneities of the structures in the concentration field generated by preferential concentration, and the existence of characteristic scales of interaction between the dispersed and carrier phases. Additionally, an interphase multiresolution analysis is performed that indicates the occurrence of a spatial anticorrelation between the enstrophy and kinetic-energy spectra of the carrier phase and the particle concentration at small scales in regimes where preferential concentration is important. In conclusion, this anticorrelation vanishes as the scale is increased and is largely suppressed when the preferential-concentration effect is negligible.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗
DOE OSTI2017

Spatial-Spectral Representation for X-Ray Fluorescence Image Super-Resolution

X-ray fluorescence (XRF) scanning of works of art is becoming an increasing popular nondestructive analyticalmethod. The high quality XRF spectra is necessary to obtain significant information on both major and minor elements used for characterization and provenance analysis. However, there is a tradeoff between the spatial resolution of an XRF scan and the signalto- noise ratio (SNR) of each pixel’s spectrum, due to the limited scanning time. In this project, we propose an XRF image super-resolution method to address this tradeoff; thus, obtaining a high spatial resolution XRF scan with high SNR. We fuse a lowresolution XRF image and a conventional RGB high-resolution image into a product of both high spatial and high spectral resolution XRF image. There is no guarantee of a one to one mapping between XRF spectrum and RGB color since, for instance, paintings with hidden layers cannot be detected in visible but can in X-ray wavelengths. We separate the XRF image into the visible and nonvisible components. The spatial resolution of the visible component is increased utilizing the high-resolution RGB image, whereas the spatial resolution of the non-visible component is increased using a total variation super-resolution method. Finally, the visible and nonvisible components are combined to obtain the final result.

Dai, Qiqin↗
NASA NTRS2003

Characterization of Aeromechanics Response and Instability in Fans, Compressors, and Turbine Blades

This study investigated the effect of interaction between tip clearance flow, steady and unsteady upstream wakes in rotor and stator blade rows in terms of blade forced response. In a stator blade row, the interaction of steady wakes in the upstream rotor frame with the stator imply a blade forced response whose spectrum contains the Blade passing frequency (BPF) and its harmonics, with a decaying amplitude as the frequency increases. When the incoming wakes are unsteady, however, the spectrum of blade excitation exhibits unexpectedly amplified high frequencies due to the modulation of BPF with the fluctuation frequency. In a rotor blade row, a tip flow instability has been demonstrated with a frequency (TVF) equal to 0.45 times the Blade Passing frequency corresponding to a reduced frequency (F(sub c) (sup +)) of 0.7. Under uniform inlet flow conditions, the frequency and spatial content of the tip flow region have been characterized. The disturbance TVF was the dominant disturbance in the flow field and was found to imply variations of the pressure coefficient of more than 30% on the blade tip (between 35% to 90% chord) and in the rotor-generated wake (from 75% to 100% hub-to-tip position). In an attempt to better understand the origin of the instability, the structure of the tip flow has also been analyzed. The interface between the tip flow region and the core flow has been found to have periodical wave-like flow patterns which proceed downstream at a speed of approximately 0.42 times the core flow speed at a frequency corresponding to TVF. A list of conclusions derived from these interactions is presented.

Tan, Choon S.↗
NASA NTRS2001

On-Orbit Performance of MODIS On-Board Calibrators

The Terra MODIS (Moderate Resolution Imaging Spectroradiometer) was launched on December 18, 1999 and acquired the first scene data on February 24, 2000. It has 36 spectral bands covering spectral range from 0.41 to 14.2 microns and provides spatial resolutions of 250 (2 bands), 500 (5 bands), and 1000 m at Nadir. The instrument on-orbit calibration and characterization are determined and monitored through the use of a number of on-board calibrators (OBC). Radiometric calibration for the reflective solar bands (B1-B19, B26), from VIS (visible) to SWIR (short wavelength infrared) (0.41 to 2.1 microns), uses a Spectralon (tm) solar diffuser (SD) and a solar diffuser stability monitor (SDSM). For the thermal emissive bands (B20-B25, B27-B36), from MWIR (medium wavelength infrared) to LWIR (long wavelength infrared) (3.75 to 14.2 micron), a V-grooved flat panel blackbody is used. The instrument spectral for the VIS to SWIR bands and spatial co-registration characterizations for all bands are monitored on-orbit by the spectral radiometric calibration assembly (SRCA). In this report, we discuss the application and performance of the key MODIS on-board calibrators and their impacts on the instrument system calibration and characterization.

Xiong, X.↗
NASA NTRS2016

Overview of LBTI: A Multipurpose Facility for High Spatial Resolution Observations

The Large Binocular Telescope Interferometer (LBTI) is a high spatial resolution instrument developed for coherent imaging and nulling interferometry using the 14.4 m baseline of the 2x8.4 m LBT. The unique telescope design, comprising of the dual apertures on a common elevation-azimuth mount, enables a broad use of observing modes. The full system is comprised of dual adaptive optics systems, a near-infrared phasing camera, a 1-5 micrometer camera (called LMIRCam), and an 8-13 micrometer camera (called NOMIC). The key program for LBTI is the Hunt for Observable Signatures of Terrestrial planetary Systems (HOSTS), a survey using nulling interferometry to constrain the typical brightness from exozodiacal dust around nearby stars. Additional observations focus on the detection and characterization of giant planets in the thermal infrared, high spatial resolution imaging of complex scenes such as Jupiter's moon, Io, planets forming in transition disks, and the structure of active Galactic Nuclei (AGN). Several instrumental upgrades are currently underway to improve and expand the capabilities of LBTI. These include: Improving the performance and limiting magnitude of the parallel adaptive optics systems; quadrupling the field of view of LMIRcam (increasing to 20"x20"); adding an integral field spectrometry mode; and implementing a new algorithm for path length correction that accounts for dispersion due to atmospheric water vapor. We present the current architecture and performance of LBTI, as well as an overview of the upgrades.

The Large Binocular Telescope Interferometer (LBTI↗
NASA NTRS2013

Suomi NPP VIIRS Prelaunch and On-orbit Geometric Calibration and Characterization

The Visible Infrared Imager Radiometer Suite (VIIRS) sensor was launched 28 October 2011 on the Suomi National Polarorbiting Partnership (SNPP) satellite. VIIRS has 22 spectral bands covering the spectrum between 0.412 m and 12.01 m, including 16 moderate resolution bands (M-bands) with a spatial resolution of 750 m at nadir, 5 imaging resolution bands (I-bands) with a spatial resolution of 375 m at nadir, and 1 day-night band (DNB) with a near-constant 750 m spatial resolution throughout the scan. These bands are located in a visible and near infrared (VisNIR) focal plane assembly (FPA), a short- and mid-wave infrared (SWMWIR) FPA and a long-wave infrared (LWIR) FPA. All bands, except the DNB, are co-registered for proper environmental data records (EDRs) retrievals. Observations from VIIRS instrument provide long-term measurements of biogeophysical variables for climate research and polar satellite data stream for the operational communitys use in weather forecasting and disaster relief and other applications. Well Earth-located (geolocated) instrument data is important to retrieving accurate biogeophysical variables. This paper describes prelaunch pointing and alignment measurements, and the two sets of on-orbit correction of geolocation errors, the first of which corrected error from 1,300 m to within 75 m (20 I-band pixel size), and the second of which fine tuned scan angle dependent errors, bringing VIIRS geolocation products to high maturity in one and a half years of the SNPP VIIRS on-orbit operations. Prelaunch calibration and the on-orbit characterization of sensor spatial impulse responses and band-to-band co-registration (BBR) are also described.

Geolocation↗
NASA NTRS1997

Application of High-Resolution Thermal Infrared Remote Sensing and GIS to Assess the Urban Heat Island Effect

Day and night airborne thermal infrared image data at 5 m spatial resolution acquired with the 15-channel (0.45 micron - 12.2 micron) Advanced Thermal and Land Applications Sensor (ATLAS) over Alabama, Huntsville on 7 September, 1994 were used to study changes in the thermal signatures of urban land cover types between day and night. Thermal channel number 13 (9.6 micron - 10.2 micron) data with the best noise-equivalent temperature change (NEAT) of 0.25 C after atmospheric corrections and temperature calibration were selected for use in this analysis. This research also examined the relation between land cover irradiance and vegetation amount, using the Normalized Difference Vegetation Index (NDVI), obtained by ratioing the difference and the sum of the red (channel number 3: 0.60-0.63 micron) and reflected infrared (channel number 6: 0.76-0.90 micron) ATLAS data. Based on the mean radiance values, standard deviations, and NDVI extracted from 351 pairs of polygons of day and night channel number 13 images for the city of Huntsville, a spatial model of warming and cooling characteristics of commercial, residential, agricultural, vegetation, and water features was developed using a GIS approach. There is a strong negative correlation between NDVI and irradiance of residential, agricultural, and vacant/transitional land cover types, indicating that the irradiance of a land cover type is greatly influenced by the amount of vegetation present. The predominance of forests, agricultural, and residential uses associated with varying degrees of tree cover showed great contrasts with commercial and services land cover types in the center of the city, and favors the development of urban heat islands. The high-resolution thermal infrared images match the complexity of the urban environment, and are capable of characterizing accurately the urban land cover types for the spatial modeling of the urban heat island effect using a GIS approach.

Lo, C. P.↗
DOE OSTI2018

Sum-Frequency Generation from a Thin Cylindrical Layer

In the Rayleigh–Gans–Debye approximation, we have solved the problem of the sum-frequency generation by two plane elliptically polarized electromagnetic waves from the surface of a dielectric particle of a cylindrical shape that is coated by a thin layer possessing nonlinear optical properties. The formulas that describe the sum-frequency field have been presented in the tensor and vector forms for the second-order nonlinear dielectric susceptibility tensor, which was chosen in the general form, containing chiral components. Expressions describing the sum-frequency field from the cylindrical particle ends have been obtained for the case of a nonlinear layer possessing chiral properties. Three-dimensional directivity patterns of the sum-frequency radiation have been analyzed for different combinations of parameters (angles of incidence, degrees of ellipticity, orientations of polarization ellipses, cylindrical particle dimensions). The mathematical properties of the spatial distribution functions of the sum-frequency field, which characterize the symmetry of directivity patterns, have been revealed.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗
DOE OSTI2025

Real-time neutron multiplicity and source localization for criticality safety during fuel debris removal

Advancing neutron detection and analysis techniques for complex radiation environments is an ongoing focus in nuclear instrumentation and monitoring. This proposal presents research and development of a generalized real-time neutron monitoring and analysis system, applicable to any detector capable of producing time-tagged neutron count data. While the work is demonstrated using the Neutron Multiplication Analysis Detector (NoMAD), a modular 15-tube helium-3 (He-3) array, due to its availability, spatial resolution, and flexible deployment, the methods developed are extensible to other systems, including organic scintillators and fast digital detectors. This research investigates two complementary analytical techniques for real-time characterization of neutron emitting sources: neutron multiplicity estimation based on the Hage-Cifarelli formalism and spatial localization using supervised machine learning applied to spatial count rate patterns. These methods are designed to operate under dynamic, evolving conditions such as fuel debris retrieval or reactor startup, where neutron-emitting material geometries may be partially unknown or changing over time. By integrating statistical neutron emission data with spatial localization, this research aims to develop and evaluate methods for real time neutron monitoring, source characterization, and material verification. Key contributions include implementation of a low-latency data pipeline for continuous neutron multiplicity analysis, development and validation of machine learning models for spatial inference, and experimental evaluation of system performance under variable measurement conditions. The outcomes are intended to support applications in nuclear safeguards, verification, emergency response, and reactor startup.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗
DOE OSTI2025

Sea ice pattern effect on Earth’s energy budget is characterized by hemispheric asymmetry

Earth’s energy budget is sensitive to the spatial distribution of sea surface temperature and sea ice concentration (SIC) change, but the global radiative effect of changes in SIC spatial distribution has not been quantified. We show that SIC-induced radiation anomalies at the top of the atmosphere are sensitive to the location of SIC reduction in each season, which qualitatively explains how and why the effect of sea ice loss on Earth’s energy budget is determined by its spatial pattern. Idealized experiments indicate that SIC-induced surface warming is greater in the Arctic regions, resulting in a more negative Planck feedback. Global low-level cloud cover responses to Arctic and Antarctic SIC reduction are also distinct, leading to more negative SIC-cloud feedback in Arctic regions. SIC-induced albedo feedback is sensitive to latitude due to inhomogeneous solar radiation at the surface. As a result, the simulated radiative effect of SIC anomalies during 1980–2019 is dominated by variations in the spatial pattern of SIC.

54 ENVIRONMENTAL SCIENCES↗
NASA NTRS1992

Characterization of YBa2Cu3O7, including critical current density Jc, by trapped magnetic field

Spatial distributions of persistent magnetic field trapped by sintered and melt-textured ceramic-type high-temperature superconductor (HTS) samples have been studied. The trapped field can be reproduced by a model of the current consisting of two components: (1) a surface current Js and (2) a uniform volume current Jv. This Js + Jv model gives a satisfactory account of the spatial distribution of the magnetic field trapped by different types of HTS samples. The magnetic moment can be calculated, based on the Js + Jv model, and the result agrees well with that measured by standard vibrating sample magnetometer (VSM). As a consequence, Jc predicted by VSM methods agrees with Jc predicted from the Js + Jv model. The field mapping method described is also useful to reveal the granular structure of large HTS samples and regions of weak links.

Chen, In-Gann↗
NASA NTRS2001

Remote Sensing of Atlanta's Urban Sprawl and the Distribution of Land Cover and Surface Temperature

Between 1973 and 1992, an average of 20 ha of forest was lost each day to urban expansion of Atlanta, Georgia. Urban surfaces have very different thermal properties than natural surfaces-storing solar energy throughout the day and continuing to release it as sensible heat well after sunset. The resulting heat island effect serves as catalysts for chemical reactions from vehicular exhaust and industrialization leading to a deterioration in air quality. In this study, high spatial resolution multispectral remote sensing data has been used to characterize the type, thermal properties, and distribution of land surface materials throughout the Atlanta metropolitan area. Ten-meter data were acquired with the Advanced Thermal and Land Applications Sensor (ATLAS) on May 11 and 12, 1997. ATLAS is a 15-channel multispectral scanner that incorporates the Landsat TM bands with additional bands in the middle reflective infrared and thermal infrared range. The high spatial resolution permitted discrimination of discrete surface types (e.g., concrete, asphalt), individual structures (e.g., buildings, houses) and their associated thermal characteristics. There is a strong temperature contrast between vegetation and anthropomorphic features. Vegetation has a modal temperature at about 20 C, whereas asphalt shingles, pavement, and buildings have a modal temperature of about 39 C. Broad-leaf vegetation classes are indistinguishable on a thermal basis alone. There is slightly more variability (+/-5 C) among the urban surfaces. Grasses, mixed vegetation and mixed urban surfaces are intermediate in temperature and are characterized by broader temperature distributions with modes of about 29 C. Thermal maps serve as a basis for understanding the distribution of "hotspots", i.e., how landscape features and urban fabric contribute the most heat to the lower atmosphere.

Laymon, Charles A.↗
NASA NTRS2001

Remote Sensing of Atlanta's Urban Sprawl and the Distribution of Land Cover and Surface Temperatures

Between 1973 and 1992, an average of 20 ha of forest was lost each day to urban expansion of Atlanta, Georgia. Urban surfaces have very different thermal properties than natural surfaces-storing solar energy throughout the day and continuing to release it as sensible heat well after sunset. The resulting heat island effect serves as catalysts for chemical reactions from vehicular exhaust and industrialization leading to a deterioration in air quality. In this study, high spatial resolution multispectral remote sensing data has been used to characterize the type, thermal properties, and distribution of land surface materials throughout the Atlanta metropolitan area. Ten-meter data were acquired with the Advanced Thermal and Land Applications Sensor (ATLAS) on May 11 and 12, 1997. ATLAS is a 15-channel multispectral scanner that incorporates the Landsat TM bands with additional bands in the middle reflective infrared and thermal infrared range. The high spatial resolution permitted discrimination of discrete surface types (e.g., concrete, asphalt), individual structures (e.g., buildings, houses) and their associated thermal characteristics. There is a strong temperature contrast between vegetation and anthropomorphic features. Vegetation has a modal temperature at about 20 C, whereas asphalt shingles, pavement, and buildings have a modal temperature of about 39 C. Broad-leaf vegetation classes are indistinguishable on a thermal basis alone. There is slightly more variability (plus or minus 5 C) among the urban surfaces. Grasses, mixed vegetation and mixed urban surfaces are intermediate in temperature and are characterized by broader temperature distributions with modes of about 29 C. Thermal maps serve as a basis for understanding the distribution of "hotspots", i.e., how landscape features and urban fabric contribute the most heat to the lower atmosphere.

Laymon, Charles A.↗
NASA NTRS2016

Uncertainties in Coastal Ocean Color Products: Impacts of Spatial Sampling

With increasing demands for ocean color (OC) products with improved accuracy and well characterized, per-retrieval uncertainty budgets, it is vital to decompose overall estimated errors into their primary components. Amongst various contributing elements (e.g., instrument calibration, atmospheric correction, inversion algorithms) in the uncertainty of an OC observation, less attention has been paid to uncertainties associated with spatial sampling. In this paper, we simulate MODIS (aboard both Aqua and Terra) and VIIRS OC products using 30 m resolution OC products derived from the Operational Land Imager (OLI) aboard Landsat-8, to examine impacts of spatial sampling on both cross-sensor product intercomparisons and in-situ validations of R(sub rs) products in coastal waters. Various OLI OC products representing different productivity levels and in-water spatial features were scanned for one full orbital-repeat cycle of each ocean color satellite. While some view-angle dependent differences in simulated Aqua-MODIS and VIIRS were observed, the average uncertainties (absolute) in product intercomparisons (due to differences in spatial sampling) at regional scales are found to be 1.8%, 1.9%, 2.4%, 4.3%, 2.7%, 1.8%, and 4% for the R(sub rs)(443), R(sub rs)(482), R(sub rs)(561), R(sub rs)(655), Chla, K(sub d)(482), and b(sub bp)(655) products, respectively. It is also found that, depending on in-water spatial variability and the sensor's footprint size, the errors for an in-situ validation station in coastal areas can reach as high as +/- 18%. We conclude that a) expected biases induced by the spatial sampling in product intercomparisons are mitigated when products are averaged over at least 7 km × 7 km areas, b) VIIRS observations, with improved consistency in cross-track spatial sampling, yield more precise calibration/validation statistics than that of MODIS, and c) use of a single pixel centered on in-situ coastal stations provides an optimal sampling size for validation efforts. These findings will have implications for enhancing our understanding of uncertainties in ocean color retrievals and for planning of future ocean color missions and the associated calibration/validation exercises.

Coastal ocean color↗
DOE OSTI2020

Distinguishing Mesoscale Polar Order (Unidirectional vs Bidirectional) of Cellulose Microfibrils in Plant Cell Walls Using Sum Frequency Generation Spectroscopy

Cellulose in plant cell walls are synthesized as crystalline microfibrils with diameters of 3–4 nm and lengths of around 1–10 μm. These microfibrils are known to be the backbone of cell walls, and their multiscale three-dimensional organization plays a critical role in cell wall functions including plant growth and recalcitrance to degradation. The mesoscale organization of microfibrils over a 1–100 nm range in cell walls is challenging to resolve because most characterization techniques investigating this length scale suffer from low spatial resolution, sample preparation artifacts, or inaccessibility of specific cell types. Here, we report a sum frequency generation (SFG) study determining the mesoscale polarity of cellulose microfibrils in intact plant cell walls. SFG is a nonlinear optical spectroscopy technique sensitive to the molecular-to-mesoscale order of noncentrosymmetric domains in amorphous matrices. However, the quantitative theoretical model to unravel the effect of polarity in packing of noncentrosymmetric domains on SFG spectral features has remained unresolved. In this work, we show how the phase synchronization principle of the SFG process is used to predict the relative intensities of vibrational modes with different polar angles from the noncentrosymmetric domain axis. Applying this model calculation for the first time and employing SFG microscopy, we found that cellulose microfibrils in certain xylem cell walls are deposited unidirectionally (or biased in one direction) instead of the bidirectional polarity which was believed to be dominant in plant cell walls from volume-averaged characterizations of macroscopic samples. Here, with this advancement in SFG analysis, one can now determine the relative polarity of noncentrosymmetric domains such as crystalline biopolymers interspersed in amorphous polymer matrices, which will open opportunities to study new questions that have not been conceived in the past.

36 MATERIALS SCIENCE↗
DOE OSTI2024

X-ray linear dichroic tomography of crystallographic and topological defects

Abstract The functionality of materials is determined by their composition 1–4 and microstructure, that is, the distribution and orientation of crystalline grains, grain boundaries and the defects within them 5,6 . Until now, characterization techniques that map the distribution of grains, their orientation and the presence of defects have been limited to surface investigations, to spatial resolutions of a few hundred nanometres or to systems of thickness around 100 nm, thus requiring destructive sample preparation for measurements and preventing the study of system-representative volumes or the investigation of materials under operational conditions 7–15 . Here we present X-ray linear dichroic orientation tomography (XL-DOT), a quantitative, non-invasive technique that allows for an intragranular and intergranular characterization of extended polycrystalline and non-crystalline 16 materials in three dimensions. We present the detailed characterization of a polycrystalline sample of vanadium pentoxide (V 2 O 5 ), a key catalyst in the production of sulfuric acid 17 . We determine the nanoscale composition, microstructure and crystal orientation throughout the polycrystalline sample with 73 nm spatial resolution. We identify and characterize grains, as well as twist, tilt and twin grain boundaries. We further observe the creation and annihilation of topological defects promoted by the presence of volume crystallographic defects. The non-destructive and spectroscopic nature of our method opens the door to operando combined chemical and microstructural investigations 11,18 of functional materials, including energy, mechanical and quantum materials.

Science & Technology - Other Topics↗
DOE OSTI2021

Electrochemical and spectroelectrochemical characterization of bacteria and bacterial systems

Microbes, such as bacteria, can be described, at one level, as small, self-sustaining chemical factories. Based on the species, strain, and even the environment, bacteria can be useful, neutral or pathogenic to human life, so it is increasingly important that we be able to characterize them at the molecular level with chemical specificity and spatial and temporal resolution in order to understand their behavior. Bacterial metabolism involves a large number of internal and external electron transfer processes, so it is logical that electrochemical techniques have been employed to investigate these bacterial metabolites. In this mini-review, we focus on electrochemical and spectroelectrochemical methods that have been developed and used specifically to chemically characterize bacteria and their behavior. First, we discuss the latest mechanistic insights and current understanding of microbial electron transfer, including both direct and mediated electron transfer. Second, we summarize progress on approaches to spatiotemporal characterization of secreted factors, including both metabolites and signaling molecules, which can be used to discern how natural or external factors can alter metabolic states of bacterial cells and change either their individual or collective behavior. Finally, we address in situ methods of single-cell characterization, which can uncover how heterogeneity in cell behavior is reflected in the behavior and properties of collections of bacteria, e.g. bacterial communities. Recent advances in (spectro)electrochemical characterization of bacteria have yielded important new insights both at the ensemble and the single-entity levels, which are furthering our understanding of bacterial behavior. Furthermore, these insights, in turn, promise to benefit applications ranging from biosensors to the use of bacteria in bacteria-based bioenergy generation and storage.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗