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Determination of Functional Capabilities, the Level of Physical Performance and the State of Main Physiological Body Systems in the First Hours after the Accomplishment of Long-term Space Flights ("Field Test")

Long-term stay in weightlessness is accompanied by alterations in the activity of main physiological body systems including sensory-motor, skeletal-muscular disturbances and cardiovascular deconditioning. However, up to now, there are no data on the state and level of functional performance of cosmonauts/astronauts directly after flight, nor are there data to help define the dynamic recovery of functional characteristics and work efficiency which are greatly needed to provide the safety and planning of their activity once they reach space objects. The Russian and American scientists are currently engaged in a joint experiment known as the "Field Test" with the goal of studying the functional performance and the state of main physiological body systems directly after landing and their temporal recovery dynamics. The functional performance is identified during the test by temporal characteristics of the movements of spatial translation, the stability of the vertical stance for 3.5 min, and the kinematic characteristics of walking - non-complicated and complicated. The following characteristics are identified as physiological characteristics of the test: a) orthostatic tolerance during stand test, b) back muscle tone; c) vertical stability - by characteristics of the correction responses to unexpected perturbations of the vertical stance, and d) support reactions during the performance of the full battery of tests. To date, a pilot version of the "Field Test" has been conducted with participation from four Russian cosmonauts. The results of studies have shown that in 1 - 5 hours after landing the functional abilities of the cosmonauts are considerably reduced. All the test movements at this time are considerably slower than preflight and the more complicated the task is, the greater significant reduction in orthostatic tolerance: during the first test that occurs 1 - 5 hours after landing. two of four cosmonauts declined to continue the task after the orthostatic test (one of them did not wear the anti-G suit "Centaurus" during testing). Blood pressure during moving out of prone posture to vertical stance in one of the cosmonauts and of sitting to standing position in the other dropped to the precollapse level. The results of the studies have confirmed the feasibility, the usefulness and the safety of conducting tests as close as possible to the landing. The program of "Field Test" experiment will be continued and extended.

Kozlovskaya, Inesa↗

Extraction of Uranium from Seawater: Design and Testing of a Symbiotic System (Final Report)

The U.S. Department of Energy in October 2014 awarded the Massachusetts Institute of Technology (MIT) a Nuclear Energy University Program grant (DE-NE0008268) to investigate the design and testing of a symbiotic system to harvest uranium from seawater. As defined in the proposal, the goals for the project are: 1. Address the design of machines for seawater uranium mining. 2. Develop design rules for a uranium harvesting system that would be integrated into an offshore wind power tower. 3. Fabricate a 1/50th size scale prototype for bench and pool-testing to verify initial analysis and theory. 4. Design, build, and test a second 1/10th size scale prototype in the ocean for more comprehensive testing and validation. This report describes work done as part of DE-NE0008268 from 10/01/2014 to 11/30/2017 entitled, “Extraction of Uranium from Seawater: Design and Testing of a Symbiotic System.” This effort is part of the Seawater Uranium Recovery Program. This report details the publications and presentations to date on the project, an introduction to the project’s goals and background research into previous work done to achieve these goals thus far. From there, the report describes an algorithm developed during the project used to optimize the adsorption of uranium by changing mechanical parameters such as immersion time and adsorbent reuses is described. Next, a design tool developed as part of the project to determine the global feasibility of symbiotic uranium harvesting systems. Additionally, the report details work done on shell enclosures for uranium adsorption. Moving on, the results from the design, building, and testing of a 1/50th physical scale prototype of a highly feasible symbiotic uranium harvester is described. Then, the report describes the results from flume experiment used to determine the affect of enclosure shells on the uptake of uranium by the adsorbent they enclose. From there the report details the design of a Symbiotic Machine for Ocean uRanium Extraction (SMORE). Next, the results of the 1/10th scale physical scale prototype of a highly feasible symbiotic uranium harvester are presented. The report then details the design and results of an experiment to examine the hydrodynamic effects of a uranium harvester on the offshore wind turbine it is attached to using a 1/150th Froude scale tow tank test. Finally, the report details the results of an initial cost-analysis for the production of uranium from seawater from such a symbiotic device.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

Extraction of Uranium from Seawater: Design and Testing of a Symbiotic System

The U.S. Department of Energy in October 2014 awarded the Massachusetts Institute of Technology (MIT) a Nuclear Energy University Program grant (DE-NE0008268) to investigate the design and testing of a symbiotic system to harvest uranium from seawater. As defined in the proposal, the goals for the project are: 1. Address the design of machines for seawater uranium mining. 2. Develop design rules for a uranium harvesting system that would be integrated into an offshore wind power tower. 3. Fabricate a 1/50th size scale prototype for bench and pool-testing to verify initial analysis and theory. 4. Design, build, and test a second 1/10th size scale prototype in the ocean for more comprehensive testing and validation. This report describes work done as part of DE-NE0008268 from 10/01/2014 to 11/30/2017 entitled, “Extraction of Uranium from Seawater: Design and Testing of a Symbiotic System.” This effort is part of the Seawater Uranium Recovery Program. This report details the publications and presentations to date on the project, an introduction to the project’s goals and background research into previous work done to achieve these goals thus far. From there, the report describes an algorithm developed during the project used to optimize the adsorption of uranium by changing mechanical parameters such as immersion time and adsorbent reuses is described. Next, a design tool developed as part of the project to determine the global feasibility of symbiotic uranium harvesting systems. Additionally, the report details work done on shell enclosures for uranium adsorption. Moving on, the results from the design, building, and testing of a 1/50th physical scale prototype of a highly feasible symbiotic uranium harvester is described. Then, the report describes the results from flume experiment used to determine the affect of enclosure shells on the uptake of uranium by the adsorbent they enclose. From there the report details the design of a Symbiotic Machine for Ocean uRanium Extraction (SMORE). Next, the results of the 1/10th scale physical scale prototype of a highly feasible symbiotic uranium harvester are presented. The report then details the design and results of an experiment to examine the hydrodynamic effects of a uranium harvester on the offshore wind turbine it is attached to using a 1/150th Froude scale tow tank test. Finally, the report details the results of an initial cost-analysis for the production of uranium from seawater from such a symbiotic device.

42 ENGINEERING↗

PNNI Performance Validation Test Report

Two Private Network-Network Interface (PNNI) neighboring peers were monitored with a protocol analyzer to understand and document how PNNI works with regards to initialization and recovery processes. With the processes documented, pertinent events were found and measured to determine the protocols behavior in several environments, which consisted of congestion and/or delay. Subsequent testing of the protocol in these environments was conducted to determine the protocol's suitability for use in satellite-terrestrial network architectures.

Dimond, Robert P.↗

Design and Technology Maturation of the Stratospheric Projectile Experiment of Entry Dynamics

The supersonic and transonic dynamic stability of blunt-body reentry vehicles currently poses large risks in all of NASA’s ongoing entry missions (MSR SRL, MSR EES, and Dragonfly). These projects have allocated millions of dollars to testing and modeling efforts to buy down risk by using the current state-of-the-art (SoA) facilities at NASA’s disposal. While these facilities have heritage in supplying dynamics data to reentry missions, their availability is severely limited – particularly with the high number of concur-rent projects requesting simultaneous testing– and are costly when considering the science density per dollar. None of the current SoA facility methodologies allow the test model to have the dynamics fully develop through a flight relevant free-stream profile and as such require extrapolations with resultant high uncertainties in order to relate the test dynamics to flight expectations. SPEED is a NASA Ames Center Innovation Fund (CIF) project that is developing a highly tailorable and cost-effective test methodology to better assess the dynamic stability of blunt-body reentry vehicles via a stratospheric balloon flight. This is accomplished by dropping a suite of instrumented capsules from a stratospheric balloon to gain a statistically relevant dataset of scaled reentry vehicles in mission relevant free-flight conditions. This presentation will walk through how the test methodology is being implemented specifically for the Mars Sample Return (MSR) Earth Entry System (EES) geometry in an awarded Flight Opportunities Program (FOP) test flight in early CY24. SPEED Application to MSR: SPEED consists of three main mechanical systems: the Drop Platform, the Projectile, and the test Capsule. SPEED is being developed as a set of guidelines and recommendations for how to test with the proposed Concept of Operations (Conops) since the specific design parameters will vary depending on the specific project’s reference trajectory and entry vehicle design. As such, this presentation will walk through the development time-line as shown in Fig. 2. This is meant to serve as a blueprint for further missions as desired. Mechanical and Avionics Design. The SPEED test platform designed for the MSR-EES capsule geometry with nominal entry parameters has the ability to carry 10 Capsules to altitude instrumented with: 1. 3-Axis Accelerometer 2. IMU 3. Gyroscope 4. Magnetometer 5. Pressure Transducer cruciform 6. Uplook and Horizon Cameras To package the avionics/instrumentation suite, the capsule is approximately 1’ in diameter with the Outer Mold Line (OML) centroid-scaled from the full EES design. The internal volume is gutted and custom-shaped to fit the desired instrumentation suite, as well as to allow for the positioning of ballast mass such that the Center of Gravity is analogous to the flight vehicle. All structural components in the Capsule and Projectile are 3D printed, which significantly reduces the cost of each flight unit to around $1500 including all instrumentation, avionics, and structural components. Flight Conops. The test Capsule is accelerated to the desired altitude and Mach number while stowed in the Projectile, a missile-like vehicle consisting of steel ballast in the nose, a low-drag OML, and an Ejection Mechanism to reliably release the Capsule into the free-flow supersonic conditions. For the MSR-EES design, the capsule employs ~3kg of ballast mass at the nose to accelerate the 1.25kg test Capsule to ~Mach 1.7 at 23km altitude. This requires an initial release altitude of 40km, the quoted limit of a 80kg payload by the FOP-contracted balloon provider. Once the Ejection Mechanism avionics detect the proper conditions, the spring-loaded Ejection Mechanism will release and – guided by the sabot – expose the test Capsule to the desired test conditions for ~5 seconds of free-flight in the supersonic/transonic regimes. Dynamics in the subsonic regime will also be captured with the instrumentation suite with post-flight recovery operations aimed at recovering the high-G-load capable SD cards after the planned hard impact landings. Testing and Development: In the few months the SPEED project has worked the development of MSR-EES flight test, the team has performed lab and drone based testing which this presentation will overview. After the first design phase, the team fabricated Engineering Demonstration Units (EDUs) of all subsystems to perform validation testing shown in Fig. 5. After validation was completed on the subsystem level, a drone-drop test was performed at the recreational flight ceiling of 400ft altitude to assess the SPEED systems in a flight environment. Parameters such as in-flight stability, hard impact landing performance, and avionics performance were quantified and qualified. The FY23 CIF will culminate in a helicopter drop test aboard an Air National Guard Blackhawk. This will prepare the team for the CY24 FOP stratospheric balloon flight that should provide the final verification to begin offering the test platform for mission support. Focus of Presentation: This presentation will outline the technology maturation path of the SPEED implementation to the MSR-EES capsule baseline as well as the details regarding the mechanical system, avionics and instrumentation, and flight operations. Note that a complementary presentation is being submitted for a methodology overview of the SPEED test platform, introducing the testing technique and benefits as well as the full application space of the technology.

pitch damping coefficient↗

A simple application of the Bailey-Orowan creep model to Fe-39.8 at. pct Al and gamma/gamma prime - alpha

The results of a study to determine the recovery rates and work-hardening coefficients for creep from constant cross-head speed compressive tests are presented. Stressing and straining rates are computed from measured time-load curves obtained from compression testing between 1200 and 1400 K of several B2 crystal structure Fe-39.8 Al intermetallic materials and the directionally solidified eutectic alloy gamma/gamma prime alpha. These quantities are then fitted to the universal form of the Bailey-Orowan equation for creep. The recovery rates were found to be functions of nominal strain rate, stress, and temperature, while the hardening coefficients were dependent only on temperature. While the work-hardening coefficient for gamma/gamma prime - alpha was about 0.05 of the elastic modulus, the work-hardening coefficients for Fe-39.8 at. pct Al were less than 0.002 of the modulus.

Whittenberger, J. D.↗

Field-Programmable Gate Array Implementation of a Single Photon-Counting Receive Modem

We present a field-programmable gate array (FPGA) implementation of a single photon-counting receive modem for a pulse position modulated signal. The modem is compliant with the Consultative Committee for Space Data Systems (CCSDS) High Photon Efficiency (HPE) Optical Communications Coding and Synchronization standard and is capable of a maximum data rate of 267 Mbps. The system is designed on a commercial off-the-shelf FPGA platform and utilizes superconducting nanowire single photon counting detectors, analog to digital converters (ADC s) to sample the detectors, and two FPGAs. Symbol timing recovery, photon counting, convolutional deinterleaving, and codeword synchronization areis performed in the first FPGA. The second FPGA performs iterative decoding on each codeword of the serially concatenated pulse position modulated (SCPPM) signal. A digital filter is included to compensate for timing jitter of the detector, and the decoder throughput can be modified adjusted through reconfigurable parallelization. The decoder also implements a resource-efficient, algorithmic polynomial interleaver and deinterleaver. Both FPGAs can be reconfigured to switch between pulse position modulation (PPM)-16 and PPM-32 with code rates 1/3, 1/2, and 2/3. In this paper, we describe the receiver architecture and FPGA implementation of the timing recovery loop and SCPPM decoder, FPGA utilization for the different modes, and receive modem characterization test results.

Field-programmable Gate Array↗

Applications Study of Aero-Maneuvering Orbit-to-Orbit Shuttle (AMOOS), Executive Summary

Preliminary designs and the supporting analysis for both the Aeromaneuvering Orbit-to-Orbit Shuttle (AMOOS) and the Aeromaneuvering Recovery System (AMRS) are presented. The AMOOS design is shown to yield from twice to almost three times the high energy, round-trip payloads as a purely propulsive vehicle of the same weight. Typically AMOOS can perform a crew rotation mission to equatorial geosynchronous orbit in one space shuttle launch. The flight test program analysis modeled parameters such as maximum dynamic pressure, heating rates, guidance, stability and recovery. Two model flight schedules were developed, one consisting of four flights and the other of two flights. The former is considered a very low risk, high information return program whereas the latter is a minimal cost program consistent with reasonable data returns and chance of success. The AMOOS and AMRS guidance scheme developed using linear regulator theory proved a precise and accurate guidance scheme. Both it and a classical linear system's based scheme were evaluated using 65 simulated trajectories in which the position in the entry corridor and the atmospheric density were varied randomly.

White, J.↗

The nonlinear rheology of complex yield stress foods

Many foods have a yield stress that allows them to retain a desired shape at rest, but transition into a viscous fluid when being served or consumed. The determination of the yield stress of the food dictates how the foods are formed and packaged, how they are served, and how they are perceived when being eaten. Oscillatory shearing provides an ideal test protocol to map the rheology across a range of time and flow strength scales. We couple oscillatory shearing and an iterative recovery procedure to show that the yielding process is a continuous transition for two common yield stress foods. We show that unrecoverable processes from oscillatory tests are equivalent to the steady shear flow behavior. We show that this yielding behavior can be well approximated by a recently published model that treats yield stress materials as continuous viscoelastic fluids with a rate-dependent relaxation time and has parameters that can be obtained from the linear viscoelastic oscillatory frequency sweep and the steady shear flow curve.

Griebler, James J.↗

Design and Testing of the X-38 Spacecraft Primary Parafoil

In August of 1995 the NASA began testing large ram-air parafoils for potential use in the landing phase of spacecraft recovery. This effort eventually became a part of the X-38 project, a technology demonstrator for the International Space Station Emergency Crew Return Vehicle. This paper traces how the original parafoil, used in the U.S. Army Graded Precision Air Delivery System (GPADS), was modified to improve the initial deployment dynamics. A discussion of how test experience with 750 sq ft parafoils has been used to scale up to the full-scale 5500 sq ft and 7500 sq ft parafoils is presented. In particular, the development of two techniques that have greatly improved the repeatability of the parafoil initial deployment and decreased the first stage deployment dynamics are discussed: a first stage upper surface energy modulator and floor vents. Comparisons of trends and results from the various scale parachutes are presented as well as constraints that at times have driven the direction that the design has taken.

DESIGN OPTIMIZATION↗

Study of fuel cell powerplant with heat recovery

It was shown that heat can be recovered from fuel cell power plants by replacing the air-cooled heat exchangers in present designs with units which transfer the heat to the integrated utility system. Energy availability for a 40-kW power plant was studied and showed that the total usable energy at rated power represents 84 percent of the fuel lower heating value. The effects of design variables on heat availability proved to be small. Design requirements were established for the heat recovery heat exchangers, including measurement of the characteristics of two candidate fuel cell coolants after exposure to fuel cell operating conditions. A heat exchanger test program was defined to assess fouling and other characteristics of fuel cell heat exchangers needed to confirm heat exchanger designs for heat recovery.

King, J. M.↗

Microbiological Sampling Methods and Sanitation of Edible Plants Grown on ISS

Pathogenic microbes on the surfaces of salad crops and growth chambers pose a threat to the health of crew on International Space Station. For astronauts to safely consume spacegrown vegetables produced in NASA's new vegetable production unit, VEGGIE, three technical challenges must be overcome: real-time sampling, microbiological analysis, and sanitation. Raphanus sativus cultivar Cherry Bomb II and Latuca sativa cultivar Outredgeous, two saled crops to be grown in VEGGIE, were inoculated with Salmonella enterica serovar Typhimurium (S. Typhimurium), a bacterium known to cause food-borne illness~ Tape- and swab-based sampling techniques were optimized for use in microgravity and assessed for effectiveness in recovery of bacteria from crop surfaces: Rapid pathogen detection and molecular analyses were performed via quantitative real-time polymerase chain reactiop using LightCycler® 480 and RAZOR® EX, a scaled-down instrument that is undergoing evaluation and testing for future flight hardware. These methods were compared with conventional, culture-based methods for the recovery of S. Typhimurium colonies. A sterile wipe saturated with a citric acid-based, food-grade sanitizer was applied to two different surface materials used in VEGGIE flight hardware that had been contaminated with the bacterium Pseudomonas aeruginosa,. another known human pathogen. To sanitize surfaces, wipes were saturated with either the sanitizer or sterile deionized water and applied to each surface. Colony forming units of P. aeruginosa grown on tryptic soy agar plates were enumerated from surface samples after sanitization treatments. Depending on the VEGGIE hardware material, 2- to 4.5-log10 reductions in colony-forming units were observed after sanitization. The difference in recovery of S. Typhimurium between tape- and swab- based sampling techniques was insignificant. RAZOR® EX rapidly detected S. Typhimurium present in both raw culture and extracted DNA samples.

Parrish, Charles H. II↗

Use of Minute-by-Minute Cardiovascular Measurements During Tilt Tests to Strengthen Inference on the Effect of Long-Duration Space Flight on Orthostatic Hypotension

Typical methodology for evaluating the effects of spaceflight on orthostatic hypotension (OH) has been survival analysis of tolerance times from 80 head-up tilt tests. However when scheduled test durations are short, there may not be enough failures to allow survival analysis to adequately estimate and compare the effects of flight phase (e.g. pre-flight, number of days post-flight), flight duration, and their interaction, as well as interactions with effects of interventions or countermeasures. The problem is exacerbated in the presence of a repeated measures design, in which subjects participate in tilt tests during various flight phases. Here we show how it is possible to dramatically improve the efficiency of statistical inference in this setting by making use of the additional information contained in minute-by-minute observations of cardiovascular parameters thought to be reflective of progression towards presyncope during tilt testing. Methods: We retrospectively examined operational tilt test (OTT; 10 -min 80 head-up tilt) data from 20 International Space Station (ISS) and 66 Shuttle astronauts 10 d before launch (L-10), on landing day (R+0) and during recovery (R+1, R+3, R+6-10) depending on the level of participation. Data from 5 ISS astronauts tested on R+0 or R+1 who used non-standard countermeasures were excluded. In addition to OTT survival time, 8 cardiovascular parameters (CP: heart rate, systolic, diastolic, and mean arterial blood pressure, pulse pressure, stroke volume, cardiac output, and total peripheral resistance) that might be predictive of progression towards presyncope were measured every minute of each OTT. Statistical analysis was predicated on a two ]stage model of causation. In the first stage, flight duration and time from landing affect the astronauts' degree of OH, which is manifested in the time trends and variation of the above CPs during OTTs. In the second stage, the behavior of these parameters directly affects OTT survival time. Actual analysis proceeded in the opposite direction. First we identified those CPs or linear combinations that best predicted OTT survival regardless of what spaceflight conditions led to OTT completion or presyncope. From these, we calculated a summary statistic (one per OTT) that best predicted survival. We then used mixed ]model regression analysis to relate changes in the summary statistic to flight phase and duration. Inference on the effects of phase, duration, and their interaction on OH follows directly from this second analysis. Results: A linear combination (W) of diastolic blood pressure (DBP) and stroke volume (SV) was found to be the best predictor of OTT survival using the complete data set of minute-by-minute observations of CPs for each OTT. Furthermore, the log-transformed standard deviation of W (Z = log SW) was found to be a strong predictor of survival in the reduced data set consisting of one observation per OTT. In other words, this measure of variability of W during an OTT was the best indicator of whether or not the subject could complete the 10-min test, with higher variability (i.e. higher values of Z) being associated with greater probability of failure. In the mixed-model regression analysis where Z was now treated as a outcome with flight phase and duration groups (ISS and STS) as predictors, we found that there was a significantly more variability in W (higher values of Z) for both groups at R+0, but with no evidence of an interaction until R+3, when the ISS group still had inflated variability, but not the STS group. Conclusions: Variability of the cardiovascular index W recovers more slowly after long-compared to short-duration spaceflight. Since high variability of W has also been shown to be predictive of OTT failure, a primary manifestation of OH, a logical conclusion is that recovery from OH also is slower after long-duration compared to short-duration spaceflights.

Feiveson, Alan H.↗

Postflight balance control recovery in an elderly astronaut: a case report

OBJECTIVE: To examine the sensorimotor adaptive response of a 77-year-old man exposed to the gravito-inertial challenges of orbital space flight. STUDY DESIGN: Prospective case study with retrospective comparisons. SETTING: NASA Neurosciences Laboratory (Johnson Space Center) and Baseline Data Collection Facility (Kennedy Space Center). PRIMARY PARTICIPANT: One 77-year-old male shuttle astronaut. INTERVENTION: Insertion into low Earth orbit was used to remove gravitational stimuli and thereby trigger sensorimotor adaptation to the microgravity environment. Graviceptor stimulation was reintroduced at landing, and sensorimotor readaptation to the terrestrial environment was tracked to completion. MAIN OUTCOME MEASURES: Computerized dynamic posturography tests were administered before and after orbital flight to determine the magnitude and time course of recovery. RESULTS: The elderly astronaut exhibited balance control performance decrements on landing day; however, there were no significant differences between his performance and that of younger astronauts tested on the same shuttle mission or on previous shuttle missions of similar duration. CONCLUSIONS: These results demonstrate that the physiological changes attributed to aging do not necessarily impair adaptive sensorimotor control processes.

Case Reports↗

Recovery of DNA from fired and unfired cartridge casings: comparison of two DNA collection methods

For over 10 years, various studies have attempted to increase the recovery of DNA from ammunition by modifying the DNA collection, extraction, purification, and amplification procedures, with varying levels of success. This study focused on the “soaking” method of Montpetit & O’Donnell [1] and the “rinse-and-swab" method of Bille et al. [2]. First, testing for the presence of exogenous DNA, 210 boxed cartridges (brass, steel, and nickel-plated) from nine manufacturers were swabbed and DNA was extracted, concentrated, and quantified. Extracts that quantified > 0 ng/µL (44 of 210) were amplified and genotyped with GlobalFiler™. Of those, only one extract yielded two alleles indicating that the manufacturing and packaging of ammunition was virtually DNA free. Next, to obtain a baseline comparison of two DNA collection methods on a non-metallic substrate and identify a suitable number of cells to spot on cartridges, different DNA input amounts of primary human adult epidermal keratinocytes (HEKa) were tested. Thereafter, 300 brass and 300 nickel-plated, cartridges were spotted with HEKa cells containing ~5 ng of DNA, fired or unfired, and processed with either method. Finally, five methods representing hybrids of the soaking and rinse-and-swab methods were tested to determine if variations of those methods could be used to increase DNA yield and recovery. The results show that the soaking method consistently yielded more DNA than the rinse-and-swab method from a non-metallic substrate. However, the comparison study demonstrated that both methods performed comparably for cartridges. On average, the soaking method recovered 0.25 ng of DNA (5.1% recovery) and the rinse-and-swab method recovered 0.28 ng (5.8% recovery). However, average recoveries were significantly different among three analysts and considerable variation in yields were observed, possibly due to storage time. Furthermore, consistent with prior reports, the DNA recovered from brass casings was only 16% of that recovered from nickel-plated casings and the average yield of DNA from fired casings was reduced to 67% of unfired casings. Moreover, DNA extracts from brass or nickel-plated casings did not appear to contain amplification inhibitors and only 30/596 appeared severely degraded. Finally, both the published rinse-and-swab and soaking methods yielded more DNA than all modifications of the two methods. Overall, both methods yielded equivalent DNA quantities. Additionally, recovery of DNA from any given cartridge casing may be dependent on storage time as well as the skill, proficiency, and experience of the analyst and may reflect stochastic effects, particularly for casings containing low copy and/or degraded DNA.

59 BASIC BIOLOGICAL SCIENCES↗

A study of short test and charge retention test methods for nickel-cadmium spacecraft cells

Methods for testing nickel-cadmium cells for internal shorts and charge retention were studied. Included were (a) open circuit voltage decay after a brief charge, (b) open circuit voltage recovery after shorting, and (c) open circuit voltage decay and capacity loss after a full charge. The investigation included consideration of the effects of prior history, of conditioning cells prior to testing, and of various test method variables on the results of the tests. Sensitivity of the tests was calibrated in terms of equivalent external resistance. The results were correlated. It was shown that a large number of variables may affect the results of these tests. It is concluded that the voltage decay after a brief charge and the voltage recovery methods are more sensitive than the charged stand method, and can detect an internal short equivalent to a resistance of about (10,000/C)ohms where "C' is the numerical value of the capacity of the cell in ampere hours.

Scott, W. R.↗

Flexor bias of joint position in humans during spaceflight

The ability to estimate ankle and elbow joint position was tested before, during, and after a 17-day spaceflight. Subjects estimated targeted joint angles during isovelocity (IsoV) joint movements with agonist muscle groups either active or relaxed. These movements included elbow extension (EE) and elbow flexion (EF), and plantarflexion (PF) and dorsiflexion (DF) of the ankle. Subjects also estimated these joint positions while moving the dynamometer at their chosen (variable) velocity (VarV) during EE and PF. For IsoV tests, no differences were observed between active and passive movements for either the ankle or elbow. Compared with those of pre-flight test days, estimates of targeted elbow joint angles were approximately 5 degrees to 15 degrees more flexed in-flight, and returned toward the pre-flight values during recovery. The spaceflight effects for the ankle were inconsistent and less prevalent than those for the elbow. The VarV PF test condition for the 120 degrees target angle at the ankle exhibited approximately 5 degrees to 7 degrees more DF target angle estimates in-flight compared with those pre- or post-flight. In contrast, during IsoV PF there was a tendency for ankle estimates to be approximately 2 degrees to 3 degrees more PF after 2-3 days exposure to spaceflight. These data indicate that during spaceflight the perception of elbow extension is greater than actuality, and are consistent with the interpretation that microgravity induced a flexor bias in the estimation of the actual elbow joint position. Moreover, these effects in joint proprioception during spaceflight were observed in individual isolated single-joint movements during tasks in which vestibular function in maintaining posture were minimal.

STS-78 Shuttle Project↗

Recovery of atmospheric parameters from the Apollo/Soyuz-ATS-F radio occultation data

As part of the Apollo-Soyuz Test Project, Apollo was tracked in the satellite-to-satellite tracking mode by ATS-F. The tracking data obtained at occultation of Apollo by the earth has been used in this study to compute atmospheric parameters like pressure and temperature. The results of the numerical inversion have been compared with data from a radiosonde station near the occultation site. Near the surface of the earth, the refractivity computed from occultation data agreed with the radiosonde-derived values to within 3%. A pressure profile deduced from the refractivity profile by using a simplified model of the atmosphere showed good agreement with radiosonde measurements.

Rangaswamy, S.↗