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Numerical classification of coding sequences

DNA sequences coding for protein may be represented by counts of nucleotides or codons. A complete reading frame may be abbreviated by its base count, e.g. A76C158G121T74, or with the corresponding codon table, e.g. (AAA)0(AAC)1(AAG)9 ... (TTT)0. We propose that these numerical designations be used to augment current methods of sequence annotation. Because base counts and codon tables do not require revision as knowledge of function evolves, they are well-suited to act as cross-references, for example to identify redundant GenBank entries. These descriptors may be compared, in place of DNA sequences, to extract homologous genes from large databases. This approach permits rapid searching with good selectivity.

Non-NASA Center↗

Fundamental catalytic challenges to design improved biomass conversion technologies

During the past ten years, there has been significant interest and investment in the study of catalytic conversion of biomass-derived feedstocks into renewable fuels and chemicals. In the United States, an estimated $25 billion has been spent by venture capitalists, industry, and government agencies during this period of time to commercialize “renewable technologies” including solar energy, wind power, batteries, and biofuels (e.g., cellulosic ethanol). Four societal factors are driving these investments including: (1) the increased price of crude oil; (2) concerns about global warming; (3) the desire to improve rural economies where biomass is produced, and; (4) national goals to become energy self-sufficient. Furthermore, underlying these efforts is the realization that lignocellulosic biomass is the only realistic, near-term and non-food-competitive source of renewable organic carbon. To this end, legislative efforts, such as the US Renewable Fuel Standards, have been implemented to create subsidies, tax credits, mandates, and loan guarantees to help bring renewable fuel technologies to market. However, the representative body of industrial efforts to this end has faced significant challenges, with several startup companies having commercialized biomass conversion technologies but having struggled to reach commercial scale; in fact, many of these companies have filed for bankruptcy. This situation is likely due to the challenges associated with scaling up unproven pioneer processes, as well as neophyte investors not understanding the decade-long time frames and the sheer amount of funding that is often required to bring chemical process technologies to market. Nevertheless, several emerging catalytic technologies have either entered the market place, or are currently demonstrating their technologies in fully integrated pilot plants. Commercial and near commercial technologies for second generation biomass conversion technologies to-date include, among others: biomass-derived jet and diesel fuel from both waste vegetable oils and ethanol; small scale production of renewable jet and diesel from landfill gases via Fischer-Tropsch synthesis; catalytic conversion of carbohydrates into gasoline and aromatics; hydropyrolysis of biomass into gasoline and diesel, and; catalytic conversion of wood into aromatics. To be successful, biomass conversion technologies must ultimately be able to compete economically with petroleum technologies, which are already operating at large commercial scales and have been practiced for decades. To this end, various factors must be considered when evaluating the potential for new processes to compete with incumbent technologies; factors such as regional variations in feedstock quality and availability, government policy, subsidies and tax rates, and the proprietary positions of the ancillary technologies that might support the process. By any measure, however, a critical metric of the economic potential of a process is its efficiency with respect to the yield of products from raw materials, and this metric of performance is directly related to atom efficiency of the underlying chemical reactions. Therefore, while promising biomass conversion technologies continue to demonstrate progress towards commercialization, there remains an important need for the catalysis community to aid in this effort by: (1) designing more active, selective and stable catalysts, and (2) elucidating a more detailed understanding of the catalytic chemistries underlying these processes. Indeed, the study of catalytic biomass conversion has grown tremendously during the past decade; and new or emerging technologies that are in the laboratory stage have allowed for biomass to be converted into a wider variety of commodity chemicals and the full range of liquid fuels that are produced from petroleum. The objective of this perspective is to highlight some of the ongoing, fundamental challenges with respect to the catalytic conversion of biomass into renewable products, and to provide insight as to how the catalysis community can overcome several of these challenges. It is our belief that, as an international academic community of catalysis researchers, we can (and must) work together to address these challenges, and to drive progress toward the production of next-generation renewable chemicals and fuels from biomass.

09 BIOMASS FUELS↗

Structural switching dynamically controls the doubly pseudoknotted Rous sarcoma virus–programmed ribosomal frameshifting element

A hallmark of retrovirus replication is the translation of two different polyproteins from one RNA through programmed –1 frameshifting. This is a mechanism in which the actively translating ribosome is induced to slip in the 5′ direction at a defined codon and then continues translating in the new reading frame. Programmed frameshifting controls the stoichiometry of viral proteins and is therefore under stringent evolutionary selection. Forty years ago, the first frameshifting stimulatory element was discovered in the Rous sarcoma virus. The ~120 nt RNA segment was predicted to contain a pseudoknot, but its 3D structure has remained elusive. Now, we have determined cryoEM and X-ray crystallographic structures of this classic retroviral element, finding that it adopts a butterfly-like double-pseudoknot fold. One “wing” contains a dynamic pyrimidine-rich helix, observed crystallographically in two conformations and in a third conformation via cryoEM. The other wing encompasses the predicted pseudoknot, which interacts with a second unexpected pseudoknot through a toggle residue, A2546. This key purine switches conformations between structural states and tunes the stability of interacting residues in the two wings. We find that its mutation can modulate frameshifting by as much as 50-fold, likely by altering the relative abundance of different structural states in the conformational ensemble of the RNA. Taken together, our structure–function analyses reveal how a dynamic double pseudoknot junction stimulates frameshifting by taking advantage of conformational heterogeneity, supporting a multistate model in which high Shannon entropy enhances frameshifting efficiency.

Science & Technology - Other Topics↗

Meteor wake in high frame-rate images--implications for the chemistry of ablated organic compounds

Extraterrestrial organic matter may have been chemically altered into forms more ameanable for prebiotic chemistry in the wake of a meteor after ablation. We measured the rate of cooling of the plasma in the meteor wake from the intensity decay just behind a meteoroid by freezing its motion in high frame-rate 1000 frames/s video images, with an intensified camera that has a short phosphor decay time. Though the resulting cooling rate was found to be lower than theoretically predicted, our calculations indicated that there would have been insufficient collisions to break apart large organic compounds before most reactive radicals and electrons were lost from the air plasma. Organic molecules delivered from space to the early Earth via meteors might therefore have survived in a chemically altered form. In addition, we discovered that relatively small meteoroids generated far-ultraviolet emission that is absorbed in the immediate environment of the meteoroid, which may chemically alter the atmosphere over a much larger region than previously recognized.

Meteoroids↗

Boundary spanning increases knowledge and action on invasive species in a changing climate

Abstract Challenges associated with global change stressors on ecosystems have prompted calls to improve actionable science, including through boundary‐spanning activities, which aim to build connections and communication between researchers and natural resource practitioners. By synthesizing and translating research and practitioner knowledge, boundary‐spanning activities could support proactive, research‐informed conservation practice, but the success of these efforts is rarely evaluated. Using repeat survey data from the Northeast Regional Invasive Species and Climate Change (NE RISCC) Management Network, a boundary‐spanning organization, we evaluate whether participating in NE RISCC affected practitioners' knowledge, actions and priorities related to invasive species management under a changing climate. Our survey results suggest that practitioners who participate in NE RISCC have greater knowledge about invasive species and climate change and are incorporating climate change in more ways into their invasive species management. We also found NE RISCC membership affected the perceived usefulness of informational resources, with NE RISCC members more frequently identifying research syntheses and targeted workshops (both are common products used by NE RISCC to translate science into practice and share manager knowledge) as useful compared to non‐members. Practitioners who participate in NE RISCC also identified somewhat different research priorities, with non‐members and short‐term members more frequently identifying range‐shifting neonative species and their impacts on native communities as higher priorities compared to long‐term NE RISCC members. NE RISCC research activities and outreach materials have consistently framed range‐shifting neonative species as comparatively low risk, suggesting that this information has influenced practitioner's perception of risk. Practical implication : Although real‐world impacts of applied ecology are notoriously difficult to quantify, this analysis illustrates that if research results are actively translated, they can affect the knowledge and actions of natural resource practitioners. These impacts illustrate the potential for boundary‐spanning efforts to address other global change challenges to conservation.

Evans, Annette E.↗

Assessing Potential Propulsion Breakthroughs

The term, propulsion breakthrough, refers to concepts like propellantless space drives and faster-than-light travel, the kind of breakthroughs that would make interstellar exploration practical. Although no such breakthroughs appear imminent, a variety of investigations into these goals have begun. From 1996 to 2002, NASA supported the Breakthrough Propulsion Physics Project to examine physics in the context of breakthrough spaceflight. Three facets of these assessments are now reported: (1) predicting benefits, (2) selecting research, and (3) recent technical progress. Predicting benefits is challenging since the breakthroughs are still only notional concepts, but kinetic energy can serve as a basis for comparison. In terms of kinetic energy, a hypothetical space drive could require many orders of magnitude less energy than a rocket for journeys to our nearest neighboring star. Assessing research options is challenging when the goals are beyond known physics and when the implications of success are profound. To mitigate the challenges, a selection process is described where: (a) research tasks are constrained to only address the immediate unknowns, curious effects or critical issues, (b) reliability of assertions is more important than their implications, and (c) reviewers judge credibility rather than feasibility. The recent findings of a number of tasks, some selected using this process, are discussed. Of the 14 tasks included, six reached null conclusions, four remain unresolved, and four have opportunities for sequels. A dominant theme with the sequels is research about the properties of space, inertial frames, and the quantum vacuum.

Millis, Marc G.↗

The Payload and Operations of the Hera Mission

On 26 September 2022, NASA’s DART mission successfully impacted on Dimorphos, the secondary of the binary asteroid Didymos. DART released the Light Italian Cubesat for Imaging of Asteroids (LICIACube) two weeks before the impact, and LICIACube flew by the asteroids three minutes after the impact. On approach, DART took images of both asteroids, which, supported by additional imagery from LICIACube, characterized the pre-impact state of the Didymos system. DART changed the orbit of Dimorphos around Didymos, reducing its orbital period by 33 minutes. DART will be followed by a detailed investigation of the Didymos system and the outcome of the impact by ESA’s Hera mission. Hera will be launched in October 2024 and arrive at Didymos in early 2027. The presentation will focus on the payload of the Hera mission and the operations at the asteroid system. The main objectives of the Hera mission are to: - Measure the mass of Dimorphos to accurately measure the momentum transfer efficiency of the DART impact. - Characterise the change of the surface of Dimorphos by DART, including the properties (and existence!) of the DART crater, to improve our understanding of impact physics and to observe unweathered material, recently exposed at the surface. - Determine the physical properties of Dimorphos, including its internal structure, to allow scaling of the impact to different types of asteroids. - Measure the dynamical and physical state of the Didymos and Dimorphos system to constrain binary formation scenarios. Hera is equipped with the following payload: Two Asteroid Framing Cameras (AFCs) for both science and navigation. There resolution is ~10-4 rad/pixel, or 40 cm/pixel for close observations from 4 km. They will provide the target global properties as well as local geomorphology and will investigate the crater and impact site. They will also measure the mass of Dimorphos through the “wobble” motion of Didymos. The Planetary ALTimeter (PALT) will measure the distance to the target and shape and topography information complementary to that from AFC images. The Thermal InfraRed Imager (TIRI) will provide information about the thermal properties of the Didymos system and spectral information in the mid-infrared (6 bands from 7 – 14 µm). The Hyperscout-H hyperspectral imager will provide mineralogical information from 25 spectral bands between 665 and 975 nm. Milani, a 6 unit cubesat, will carry a visible to near-IR imaging spectrometer (ASPECT, 500 – 2500 nm) to derive mineralogical information on the composition of the asteroids, and a thermogravimeter (VISTA) to measure the abundance and constrain the composition of ambient dust particles. Juventas, a 6 unit cubesat, will carry a monostatic low-frequency radar (JuRa), and a gravimeter (GRASS) to derive interior and surface properties of the asteroids. The Radio Science experiment will measure the gravity field of the Didymos system. Measurements of the acceleration of the Hera spacecraft by the asteroid pair through the radio link between Earth and Hera will be used as well as the inter-satellite link between Hera and the two cubesats. We will describe how the goals of Hera will be achieved with the different payload elements.

Michael Küppers↗

Space Station module Power Management And Distribution (PMAD) system

This project consists of several tasks which are unified toward experimentally demonstrating the operation of a highly autonomous, user-supportive power management and distribution system for Space Station Freedom (SSF) habitation/laboratory modules. This goal will be extended to a demonstration of autonomous, cooperative power system operation for the whole SSF power system through a joint effort with NASA's Lewis Research Center, using their Autonomous Power System. Short term goals for the space station module power management and distribution include having an operational breadboard reflecting current plans for SSF, improving performance of the system communications, and improving the organization and mutability of the artificial intelligence (AI) systems. In the middle term, intermediate levels of autonomy will be added, user interfaces will be modified, and enhanced modeling capabilities will be integrated in the system. Long term goals involve conversion of all software into Ada, vigorous verification and validation efforts and, finally, seeing an impact of this research on the operation of SSF. Conversion of the system to a DC Star configuration is now in progress, and should be completed by the end of October, 1989. This configuration reflects the latest SSF module architecture. Hardware is now being procured which will improve system communications significantly. The Knowledge-Based Management System (KBMS) is initially developed and the rules from FRAMES have been implemented in the KBMS. Rules in the other two AI systems are also being grouped modularly, making them more tractable, and easier to eventually move into the KBMS. Adding an intermediate level of autonomy will require development of a planning utility, which will also be built using the KBMS. These changes will require having the user interface for the whole system available from one interface. An Enhanced Model will be developed, which will allow exercise of the system through the interface without requiring all of the power hardware to be operational. The functionality of the AI systems will continue to be advanced, including incipient failure detection. Ada conversion will begin with the lowest level processor (LLP) code. Then selected pieces of the higher level functionality will be recorded in Ada and, where possible, moved to the LLP level. Validation and verification will be done on the Ada code, and will complete sometimes after completion of the Ada conversion.

Walls, Bryan↗

Application of Digital Image Correlation in Wind Tunnel

Digital image correlation (DIC) is a non-contact measurement technique that has been used in various applications in multiple industries, from microscopic specimens all the way to large scale structures. This project involved using DIC to capture wing deflection under simulated wind loading at various angles of attack (AoA). A subscale model of a fixed-wing aircraft, shown in Figure 1, was used in the 14-footby 22-footsubsonicwind tunnel at the NASA Langley Research Center. The setup used two pairs of cameras mounted on the ceiling of the tunnel to view the starboard and port side wings. Aluminum framing was used to rigidly mount the cameras to beams in the ceiling. Camera setup challenges due to the confined space included obtaining even lighting and working around infrared (IR) cameras that were used to capture the flow over the wing. Remote connection was used to access data acquisition computers from computers in the control room. Eight measurement locations on each wing were made of 1-inch-diametercircular silver dry transfer decals that did not alter the air flow across the wing. These circles were speckled with a black ink pen and were used for the IR camera as well as for DIC systems. A mock setup outside the wind tunnel was used to optimize the camera setup and verify that the speckled tape provided sufficient correlation. DIC was used to track the position of seven locations as the AoA of the subscale model changed from −10 degrees to +10 degrees in 1-degree increments. The model was rotated at a fixed height about the center of the fuselage from −7 degrees to +10 degrees. The height of the model had to be lowered to achieve AoA slower than −7degrees due to geometric constraints of the cart supporting the model and the wind tunnel. The source of light was stationary relative to the model, so the exposure time had to be adjusted for different wing regions and AoA values. The three-dimensional (3D) visualization of the markings and the two-dimensional (2D) projection of these points onto the port wing at 0-degree AoA is shown in Figure 2.

Matlock Mennu↗

Astrodynamics Convention and Modeling Reference for Lunar, Cislunar, and Libration Point Orbits

The purpose and direction of this document is to provide U.S. government agencies, specifically National Aeronautics and Space Administration (NASA) and Department of Defense (DoD) space related centers, with a foundational summary of astrodynamics concepts for trajectory design, navigation, and operations in the cislunar, lunar, and libration point regions. This document is provided in response to an Interagency Agreement (IAA) between NASA and the National Geospatial-Intelligence Agency (NGA). With applications to these regions of the Earth-Moon system, this document summarizes: the definitions of standard and unique coordinate systems for Positioning, Navigation, Timing and targeting (PNT), transformations between those coordinate frames, definitions of common time systems, a description of numerical integration, description of a widely-used and approximate dynamical model of a three-body system for preliminary analysis and nomenclature definition, description of higher-fidelity models of cislunar space, and the application of these concepts to sample scenarios with a focus on common steps in trajectory and maneuver design for a spacecraft in cislunar space. This information is critical to mission design and navigation far above the geosynchronous orbit region, where lunar perturbations are required to be modeled accurately and consistently but render trajectory design and analysis a complex procedure. Software tools such as the Goddard Space Flight Center (GSFC) open source General Mission Analysis Tool (GMAT) is used as a reference, along with a wide variety of resources constructed by NASA and other government agencies, academia, and industry, for mathematical specifications and practical considerations. This document has been prepared by and under the auspices of NASA. The GSFC Mission Engineering and Systems Analysis (MESA) Division (Code 590) and the Navigation and Mission Design Branch (Code 595) are part of NASA. Their engineers and scientists have expertise in lunar, cislunar, and libration point region trajectory guidance and navigation and timing. NASA GSFC has supported many successful lunar and cislunar missions over the past several decades. These missions include the Lunar Reconnaissance Orbiter (LRO), the two Acceleration, Reconnection, Turbulence and Electrodynamics of the Moon’s Interaction with the Sun (ARTEMIS) spacecraft, Transiting Exoplanet Survey Satellite (TESS), Lunar Prospector, Lunar Crater Observation and Sensing Satellite (LCROSS), Clementine, and several Sun-Earth libration point missions such as WIND and Deep Space Climate Observatory (DSCOVR), dating back four decades. NASA GSFC also supports the upcoming Gateway lunar mission, the Artemis Lunar Program and Human Landing Systems, and leads both the Lunar IceCube low thrust mission and concept design for the Lunar Communication Relay and Navigation System (LCRNS).

Lunar, CisLunar, Libration, trajectory dynamics, p↗

Geostationary Operational Environmental Satellite (GOES-N report). Volume 1: Technical

The GOES-N study consisted of five distinct tasks including: determining replication costs of GOES I-M and GOES-7 in the GOES-N time frame; defining and evaluating modifications to GOES I-M to improve efficiency or reduce costs; and defining evolutionary changes to the GOES I-M design to satisfy National Weather Service (NWS) 1983 and NOAA 1989 requirements. The categorization and disposition of NOAA requirements is reported in volume 1 section 4. Results of the GOES I-M efficiency/cost improvement modifications study are described in Section 7.1. The system concept options 1, 2, and 3 that generally represent the results of the Task 2, 3A, and 3B studies are summarized in Section 7.2. Another result of the GOES-N study, the determination of which NWS 1983 and NOAA 1989 requirements can be met with the three options, is contained in volume 1 section 7. Conclusions and recommendations are covered in volume 1 section 8. Imager, sounder, control system, space environment monitor, search and rescue, weather facsimile, data collection system, and products/process/communications recommendations were extracted from sections 9, 10, and 11. Section 8 also contains conclusions pertaining to programmatic operational satellite issues (prerequisite development strategies, the direct procurement of instruments by the government, protoflight mission, etc.). Sections 9, 10, and 11 address instrument, control system, image/navigation/registration, and other system design considerations and surveys. These sections are supported by the appendices in volume 2.

Source record↗

Using CFD as Rocket Injector Design Tool: Recent Progress at Marshall Space Flight Center

The choice of tools used for injector design is in a transitional phase between exclusive reliance on the empirically based correlations and extensive use of computational fluid dynamics (CFD). The Next Generation Launch Technology (NGLT) Program goals emphasizing lower costs and increased reliability have produced a need to enable CFD as an injector design tool in a shorter time frame. This is the primary objective of the Staged Combustor Injector Technology Task currently under way at Marshall Space Flight Center (MSFC). The documentation of this effort begins with a very brief status of current injector design tools. MSFC's vision for use of CFD as a tool for combustion devices design is stated and discussed with emphasis on the injector. The concept of the Simulation Readiness Level (SRL), comprised of solution fidelity, robustness and accuracy, is introduced and discussed. This quantitative measurement is used to establish the gap between the current state of demonstrated capability and that necessary for regular use in the design process. MSFC's view of the validation process is presented and issues associated with obtaining the necessary data are noted and discussed. Three current experimental efforts aimed at generating validation data are presented. The importance of uncertainty analysis to understand the data quality is also demonstrated. First, a brief status of current injector design tools is provided as context for the current effort. Next, the MSFC vision for using CFD as an injector design tool is stated. A generic CFD-based injector design methodology is also outlined and briefly discussed. Three areas where MSFC is using injector CFD analyses for program support will be discussed. These include the Integrated Powerhead Development (IPD) engine which uses hydrogen and oxygen propellants in a full flow staged combustion (FFSC) cycle and the TR-107 and the RS84 engine both of which use RP-1 and oxygen in an ORSC cycle. Finally, an attempt is made to objectively summarize what progress has been made at MSFC in enabling CFD as an injector design tool.

Tucker, Kevin↗

Enabling Principles for Dual Participation by Energy Storage as a Transmission and Market Asset

Because the electric transmission system is sized to meet the highest levels of customer demand, much of its designed capacity goes unused during average operating conditions. As a uniquely flexible and scalable asset capable of providing multiple grid services, energy storage can be deployed to support the transmission system and then also provide services to energy markets when not needed for transmission. This paper reviews the technical barriers in transmission planning practices and energy market design that prevent the realization of dual-use energy storage projects that provide both regulated transmission and competitive market services. Current practices create barriers against the identification of energy storage in the transmission planning process and against the participation of a regulated transmission asset in competitive energy markets. A review of regional transmission plans identifies best practices for including energy storage in the planning process by directing planners to proactively identify such projects or by providing clear mechanisms for stakeholders to propose them. Creating a market participation model for dual-use energy storage assets requires establishing when an asset will be allowed to participate in the market, how it will participate in the market, and where it will recover its costs. In answering these questions, key principles must be satisfied, such as prioritizing reliability, incrementally building on existing market design, and balancing competing objectives. Several policy options exist for satisfying those principles, giving grid operators and stakeholders significant flexibility in developing a dual-use participation model in accordance with regional policies and conditions. By objectively framing the issue and identifying policy options, this paper may inform subsequent proceedings on dual-use energy storage and be a resource to regional market operators, utilities, developers, regulators, policymakers, and other stakeholders as they collectively work on this complex issue.

24 POWER TRANSMISSION AND DISTRIBUTION↗

Hippocampal place-cell firing during movement in three-dimensional space

"Place" cells of the rat hippocampus are coupled to "head direction" cells of the thalamus and limbic cortex. Head direction cells are sensitive to head direction in the horizontal plane only, which leads to the question of whether place cells similarly encode locations in the horizontal plane only, ignoring the z axis, or whether they encode locations in three dimensions. This question was addressed by recording from ensembles of CA1 pyramidal cells while rats traversed a rectangular track that could be tilted and rotated to different three-dimensional orientations. Cells were analyzed to determine whether their firing was bound to the external, three-dimensional cues of the environment, to the two-dimensional rectangular surface, or to some combination of these cues. Tilting the track 45 degrees generally provoked a partial remapping of the rectangular surface in that some cells maintained their place fields, whereas other cells either gained new place fields, lost existing fields, or changed their firing locations arbitrarily. When the tilted track was rotated relative to the distal landmarks, most place fields remapped, but a number of cells maintained the same place field relative to the x-y coordinate frame of the laboratory, ignoring the z axis. No more cells were bound to the local reference frame of the recording apparatus than would be predicted by chance. The partial remapping demonstrated that the place cell system was sensitive to the three-dimensional manipulations of the recording apparatus. Nonetheless the results were not consistent with an explicit three-dimensional tuning of individual hippocampal neurons nor were they consistent with a model in which different sets of cells are tightly coupled to different sets of environmental cues. The results are most consistent with the statement that hippocampal neurons can change their "tuning functions" in arbitrary ways when features of the sensory input or behavioral context are altered. Understanding the rules that govern the remapping phenomenon holds promise for deciphering the neural circuitry underlying hippocampal function.

Non-NASA Center↗

Segmentation and tracking in echocardiographic sequences: active contours guided by optical flow estimates

This paper presents a method for segmentation and tracking of cardiac structures in ultrasound image sequences. The developed algorithm is based on the active contour framework. This approach requires initial placement of the contour close to the desired position in the image, usually an object outline. Best contour shape and position are then calculated, assuming that at this configuration a global energy function, associated with a contour, attains its minimum. Active contours can be used for tracking by selecting a solution from a previous frame as an initial position in a present frame. Such an approach, however, fails for large displacements of the object of interest. This paper presents a technique that incorporates the information on pixel velocities (optical flow) into the estimate of initial contour to enable tracking of fast-moving objects. The algorithm was tested on several ultrasound image sequences, each covering one complete cardiac cycle. The contour successfully tracked boundaries of mitral valve leaflets, aortic root and endocardial borders of the left ventricle. The algorithm-generated outlines were compared against manual tracings by expert physicians. The automated method resulted in contours that were within the boundaries of intraobserver variability.

Non-NASA Center↗

Flight Mechanics Modeling and Simulation of the Earth Entry System

Introduction: The Mars Sample Return (MSR) Campaign being planned by NASA and ESA has the ambitious goal to return Mars samples back to Earth. This international collaboration had developed a concept of operations that included a ESA-designed Earth Return Orbiter (ERO) and NASA-designed Capture, Containment, and Return System (CCRS). The Earth Entry System (EES), consisting of a protective aeroshell that houses the samples as well as sample containment vessels, would conduct entry, descent, and landing (EDL) on a direct Earth trajectory. The EES would enter on a spin-stabilized ballistic trajectory with the goal to passively achieve aerodynamic stability throughout all regions of flight. The EDL sequence would end with the EES impacting the soft playa soil of the Utah Test and Training Range (UTTR). As of the submission of this abstract, the MSR campaign is undergoing a re-architecture leading to a pause in EES development. However, the novel approaches developed in flight mechanics modeling and simulation can significantly benefit the greater IPPW community in the development of Earth return vehicles. This paper will present the latest state of EES flight mechanics modeling and simulation. The paper will highlight the simulation architecture developed and key lessons learned from understanding of EDL trajectory sensitivities. Modeling and Simulation: Figure 1 provides a high-level concept of operations for the approach, entry, descent, and landing (AEDL) phase of the CCRS-portion of MSR. The objective of EES flight mechanics is to model and simulate the EES trajectory from ERO separation to ground impact at UTTR. A variety of flight mechanics simulation models were utilized to model both exo-atmopsheric and atmospheric portions of flight. 42, a 6-DOF simulation developed at Goddard Space Flight Center, is utilized for propagating the attitude of EES during exo-atmospheric flight. 42 allows for a variety of spin eject mechanism scenarios to be simulated for analysis. 10 minutes prior to entry, the 42 states are handed off to the EDL sims. The prime EDL sim utilized by EES is the Program to Optimize Simulated Trajectories II (POST2), a 6-DOF sim developed at Langley Research Center, and the independent verification and validation EDL sim utilized is DSENDS, a 6-DOF sim developed at Jet Propulsion Laboratory. Figure 2 provides a visualization of the flight mechanics simulation model flow through various points in the AEDL phase. Due to the existence of a variety of sim models, the EES flight mechanics team developed processes for data hand-off. These processes included the development of a centralized coordinate frame document, utilization of a single, centralized simulation input document for all sims to reference, and hand-off files containing both the technical data to be ingested by other flight mechanics sims as well as annotations of modeling assumptions utilized to generate the data. Figure~\ref{fig:post2simarchitecture} provides an overview of the POST2 sim architecture wherein POST2 ingests numerous subsystem models and input files. The dispersed state file generated by MONTE provides the position/velocity state of the trajectory while the 42 Handoff file provides the attitude. The aerodynamics database, delivered by the EES aeroscience team, is utilized to simulate the aerodynamic forces and moments experienced during EDL. A custom atmosphere model, developed by EES atmosphere team, is utilized to simulate the anticipated atmosphere environment around the region of Earth through which the EES trajectory flys. These inputs and subsystem models can be varied depending on the AEDL flight mechanics scenario being simulated. Monte Carlo simulations are utilized to generate statistical AEDL performance metrics in the form of scorecards and violin plots. Furthermore, outputs from the POST2 simulation are utilized for follow-on analyses including aerothermal and landing performance. \section{Flight Mechanics Lessons Learned} Though the EES flight mechanics team uncovered a variety of lessons learned through the analysis conducted to support CCRS through preliminary design review, this paper will highlight the most important lessons. A key AEDL performance goal is to ensure the landing footprint of EES remains on the UTTR south range. A common modeling strategy used in EDL analysis is One-Variable-At-a-Time (OVAT). OVAT analysis provides insight into the key drivers that affect AEDL performance metrics. Figure 3 shows the landing ellipses for single dispersion sources as compared to the baseline aggregate of all dispersions. The figure shows that atmosphere winds alone dominate the size of the footprint ellipse (note: EES does not use a parachute unlike previous Earth-return missions and is in wind-driven free fall for ~5min). The significance of the wind led the EES flight mechanics team to pursue the development of a Custom Atmosphere Model [4], in lieu of EarthGRAM [1], built on actual radiosonde wind measurements around the UTTR-region. This decision was driven by the realism in the generated footprint ellipses and lessons-learned from Stardust [5]. These findings will be invaluable for future Earth-return missions in providing an early understanding of the key drivers affecting footprint size and modeling considerations for which to account. Another lesson learned is tied to the AEDL performance goal of achieving passive stability throughout all regions of flight. It is well understood that blunt-body aeroshells are less stable as they transition from supersonic to subsonic. Eliminating a backshell does help improvestability; however, other phenomena such as roll-induced instability during terminal descent can still arise. The EES flight mechanics team developed stability metrics as tools to better understand the causes of and better predict the onset of dynamic instability. These tools were built upon analytical models developed by Jaffe [3] and Murphy [2]. The tools were shown to both be very accurate in correlation with actual unstable cases and useful in developing stability margin policies based on the vehicle design and simulation considerations (e.g. sphere-cone angle change, mass change, wind turbulence). These tools allowed for the current EES design to demonstrate the ability to achieve passive stability and can be an invaluable tool for consideration in the design of parachute-less Earth-return vehicles.

Rohan Deshmukh↗

Milone eTape Liquid Level Sensor Laboratory Calibration with a Commercial 12.2 cm HS-Flume and 3D Printed 4 cm Micro-HS-Flumes

The NGEE Arctic Rainfall Simulator (NARS) is a variable intensity rainfall simulator (RFS) with a frame design based on the Humphry et al. (2002) RFS and a water delivery system based on the Walnut Gulch (Paige et al., 2004) RFS. The NARS uses an aluminum frame that is fully deconstructable for transportation to field locations and a water system that enables variable rain intensity. Prior to field deployment, H-flume discharge and the corresponding Milone eTape Liquid Level Sensor (eTape) resistance values were measured in the laboratory so that discharge measurements from the NGEE Arctic Rainfall Simulator could be automated. eTape resistance was measured with increasing fluid heights for the full range of the eTape to calculate sensor detection limits and resolution. The eTape was calibrated to both a commercially available 12.2 cm HS-flume and a 3D printed 4 cm Micro-HS-flume. This data package contains two .csv files, one for the eTape calibration and the other for the flume calibrations, and two .stl files to 3D print the 4 cm Micro-HS-Flume design with a 1 cm x 3.6 cm stilling well opening. The .stl files can be opened in most 3D printing or CAD programs/software. Two .kml files of the laboratory location and broader area where testing was conducted are also included in this data package.The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a research effort to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research.The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) and the North Slope near Utqiagvik (Barrow), Alaska and 2) multiple areas on the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska.Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES↗