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Volatiles Evolved from Soybean Products Intended for Use in Long Duration Space Missions

Soybeans have been baselined to be grown in a habitat (Advanced Life Support Systems Integration Test Bed, ALSSITB) intended for evaluating advanced life support systems developed for long duration missions to the Moon or Mars. The ALSSITB is being constructed at NASA-Johnson Space Center and is composed of 5 chambers (4.6 m x 11.3 m each) and an airlock joined by an interconnecting tunnel (3.7 m x 19.2 m). Processed soy products such as soy milk and soy bread are planned to be incorporated into a nutritionally sound, plant-based food system. Since all consumables will be recycled and reused, volatile compounds evolved during the manufacturing of these food products need to be quantified to assess their impact on this closed loop system. Soy milk was made in a prototype machine and bread in a commercial bread baking machine. These machines were each placed in a tightly closed chamber and, at the completion of the process, air volatiles were identified and quantified by GC/MS. For soy milk, ethanol, acetaldehyde, methanol, hexanal, propanal and acetone and for soybread, acetaldehyde, ethanol, N-propanol and ethyl acetate were detected in significant quantities. The crew members will spend an average of 180 days in the ALSSITB and it was estimated that 138 batches of soy milk will be processed in the tunnel and 130 loaves of soybread would be baked in the habitat chamber during their stay. The aforementioned volatiles would surpass the 180 day Spacecraft Maximum Allowable Concentrations (SMACs) if no means of scrubbing are adapted which would lead to toxic levels of these compounds. Therefore, sufficient means for eliminating the contribution of volatiles evolved from food processing and preparation equipment needs to be provided in the ALSSITB.

Vodovotz, Yael↗

Sustainable Crop Cultivation in Space Analogs: A BRIDGES Methodology Perspective Through SpaCEA

Sustainable crop cultivation in space holds paramount significance for the support of life in future long-duration missions. This research explores the development and integration of innovative low-cost proof-of-concept (LC-POC) plant growth cabinets tailored for use in space analog missions. By outlining past and current efforts in space farming, this study introduces the SpaCEA Cabinet using BRIDGES framework, establishing a context for reproducible experiments and innovation in plant growth systems. The SpaCEA cabinets can either be delivered in flat packs or assembled on-site, employing cutting-edge methods like 3-D printing and laser cutting. The main objective is to assess how effectively these structures foster crop growth within analog environments while replicating conditions crucial for space exploration. Employing a multi-faceted approach encompassing technical and qualitative dimensions, this project integrates a qualitative investigation where representatives managing analog stations and analog astronauts will partake in interviews and questionnaires to discern specific requirements and challenges within these environments. Insights gained from these engagements will significantly define the final design parameters of updated SpaCEA plant growth cabinets. The practical applicability of these cabinets emphasizes ease of assembly and transportation, addressing the inherent spatial and logistical constraints associated with space missions. Furthermore, the BRIDGES framework ensures the standardization of hardware, software, and data-gathering elements within a unified structure, which utilizes cutting-edge manufacturing technologies for the prototyping and deployment of these cabinets. The anticipated outcomes of this research include the identification of key design considerations and technical specifications for plant growth cabinets tailored to space farming analog systems. This research is poised to contribute valuable knowledge to sustainable space exploration through the development of interoperable plant growth systems for analog environments, advancing research in space crop cultivation which will make up part of a larger bioregenerative life support system.

Advanced Life Support↗

Sustainable Crop Cultivation in Space Analogs: A BRIDGES Methodology Perspective Through SpaCEA

Sustainable crop cultivation in space holds paramount significance for the support of life in future long-duration missions. This research explores the development and integration of innovative low-cost proof-of-concept (LC-POC) plant growth cabinets tailored for use in space analog missions. By outlining past and current efforts in space farming, this study introduces the SpaCEA Cabinet using BRIDGES framework, establishing a context for reproducible experiments and innovation in plant growth systems. The SpaCEA cabinets can either be delivered in flat packs or assembled on-site, employing cutting-edge methods like 3-D printing and laser cutting. The main objective is to assess how effectively these structures foster crop growth within analog environments while replicating conditions crucial for space exploration. Employing a multi-faceted approach encompassing technical and qualitative dimensions, this project integrates a qualitative investigation where representatives managing analog stations and analog astronauts will partake in interviews and questionnaires to discern specific requirements and challenges within these environments. Insights gained from these engagements will significantly define the final design parameters of updated SpaCEA plant growth cabinets. The practical applicability of these cabinets emphasizes ease of assembly and transportation, addressing the inherent spatial and logistical constraints associated with space missions. Furthermore, the BRIDGES framework ensures the standardization of hardware, software, and data-gathering elements within a unified structure, which utilizes cutting-edge manufacturing technologies for the prototyping and deployment of these cabinets. The anticipated outcomes of this research include the identification of key design considerations and technical specifications for plant growth cabinets tailored to space farming analog systems. This research is poised to contribute valuable knowledge to sustainable space exploration through the development of interoperable plant growth systems for analog environments, advancing research in space crop cultivation which will make up part of a larger bioregenerative life support system.

Advanced Life Support↗

Space Station Biological Research Project

NASA Ames Research Center is responsible for the development of the Space Station Biological Research Project (SSBRP) which will support non-human life sciences research on the International Space Station Alpha (ISSA). The SSBRP is designed to support both basic research to understand the effect of altered gravity fields on biological systems and applied research to investigate the effects of space flight on biological systems. The SSBRP will provide the necessary habitats to support avian and reptile eggs, cells and tissues, plants and rodents. In addition a habitat to support aquatic specimens will be provided by our international partners. Habitats will be mounted in ISSA compatible racks at u-g and will also be mounted on a 2.5 m diameter centrifuge except for the egg incubator which has an internal centrifuge. The 2.5 m centrifuge will provide artificial gravity levels over the range of 0.01 G to 2 G. The current schedule is to launch the first rack in 1999, the Life Sciences glovebox and a second rack early in 2001, a 4 habitat 2.5 in centrifuge later the same year in its own module, and to upgrade the centrifuge to 8 habitats in 2004. The rodent habitats will be derived from the Advanced Animal Habitat currently under development for the Shuttle program and will be capable of housing either rats or mice individually or in groups (6 rats/group and at least 12 mice/group). The egg incubator will be an upgraded Avian Development Facility also developed for the Shuttle program through a Small Business and Innovative Research grant. The Space Tissue Loss cell culture apparatus, developed by Walter Reed Army Institute of Research, is being considered for the cell and tissue culture habitat. The Life Sciences Glovebox is crucial to all life sciences experiments for specimen manipulation and performance of science procedures. It will provide two levels of containment between the work volume and the crew through the use of seals and negative pressure. The glovebox will accommodate use by two crew persons simultaneously and the capability for real time video down-link and data acquisition. In house testbeds and Phase B studies of the centrifuge validated the concepts of vibration isolation and autobalancing systems to meet the ISSA microgravity requirements. The vibration isolation system is effective above the centrifuge rotation frequency while the autobalancing system on the rotor removes vibration at and below the rotation rate. Torque of the Station, induced by spin-up/spindown of the centrifuge, can be minimized by controlling spin-up/spin-down rates. The SSBRP and ISSA will provide the opportunity to perform long-term, repeatable and high quality science. The long duration increments available on the Station will permit multigeneration studies of both plants and animals which have not previously been possible. The u-g habitat racks and the eight habitat centrifuge will accommodate sufficient number of specimens to permit statistically significant sampling of specimens to investigate the time course of adaptation to altered gravity environments. The centrifuge will, for the first time, permit investigators to use gravity itself as a tool to investigate fundamental processes, to investigate the intensity and duration of gravity to maintain normal structure and function, to separate the effects of u-g from other environmental factors and to examine artificial gravity as a potential countermeasure for the physical deconditioning observed during space flight.

Johnson, Catherine C.↗

2018 NISAR Applications Workshop: Wetlands; Workshop Report

Wetland ecosystems are a critical part of our natural environment, providing socioeconomic benefits to human communities and habitats to a rich diversity of plant and animal life. Socioeconomic benefits include improved water quality, flood control, foods, shoreline stabilization, groundwater recharge, and recreational opportunities. Wetlands also have a major role as carbon sinks and sources through processes that are influenced by the duration and timing of soil saturation and inundation. Thus, carbon and water cycle models must take into account wetland extent and seasonal patterns of wetland inundation. The joint NASA, US Geological Survey (USGS) and Fish and Wildlife Service (FWS) workshop focused on advancing wetland applications of the spaceborne NASA-ISRO Synthetic Aperture Radar (SAR) mission (NISAR), a jointly developed satellite between NASA and the Indian Space Research Organisation (ISRO) expected for launch early 2022. Participants from 15 national and international organizations --including US Federal Agencies, nonprofits, academics, and the private sector-- had been identified as key-players in facilitating integration of Earth Observations into decision support workflows. Discussions were held over two and a half days to convey the knowledge and measurement needs of the wetlands community and discuss the delivery of relevant geospatial products that could be derived from NISAR data. While the community typically characterizes wetlands by their hydrological process, vegetation and soil types, a central defining characteristic is that a wetland is a land area inundated or saturated in the rootzone for at least 2 weeks of the average vegetation growing season.

FWS↗

Mars Technologies Spawn Durable Wind Turbines

Sometimes referred to as regenerative life support systems, the concept includes an enclosed self-sufficient habitat that can independently support life for years on end. Such a system aims not only to produce its own food and water but to purify air and convert waste into useful byproducts. In the early 1990s, NASA was planning for an extended stay on Mars, and Bubenheim and his Ames colleagues were concentrating efforts on creating a complete ecological system to sustain human crewmembers during their time on the Red Planet. The main barrier to developing such a system, he says, is energy. Mars has no power plants, and a regenerative system requires equipment that runs on electricity to do everything from regulating humidity in the atmosphere to monitoring the quality of recycled water. The Ames group started looking at how to best make power on a planet that is millions of miles away from Earth and turned to a hybrid concept combining wind and solar power technologies. The reason was that Mars experiences frequent dust storms that can block nearly all sunlight. When theres a dust storm and the wind is blowing, the wind system could be the dominant power source. When the wind is not blowing and the sun is out, photovoltaics could be the dominant source, says Bubenheim.To develop and test the wind power technology, Ames turned to a remote, harsh environment here on Earth: the South Pole. The South Pole was a really good analog for Mars, says Bubenheim. The technology features for going to Mars were the same technology features needed to make something work at the South Pole.Around the same time that NASA started investigating energy technologies for the Red Planet, the National Science Foundation (NSF) was working on a redesign of their station at the South Pole. To power its operations, NSF used fuel that it flew to the remote location, but the Foundation recognized the benefits of also using onsite renewable energy technologies. In the winter they have small crews and their power requirements are less, says Bubenheim. In the summers, they bring in larger groups and photovoltaics could supply a lot of power. Using renewable energy technology could be a way of reducing the amount of fuel they have to fly in.Technology TransferTo advance wind turbine technology to meet the requirements of extremely harsh environments like that on Mars, Ames partnered with NSF and the Department of Energy. It was clear that a lot of the same features were also desirable for the cold regions of the Earth, says Bubenheim. NASA took the leadership on the team because we had the longest-term technology a Mars turbine. Years before, NSF had worked with a company called Northern Power Systems (NPS), based in Barre, Vermont, to deploy a 3-kilowatt wind turbine on Black Island off the coast of Antarctica.Sometimes referred to as regenerative life support systems, the concept includes an enclosed self-sufficient habitat that can independently support life for years on end. Such a system aims not only to produce its own food and water but to purify air and convert waste into useful byproducts. In the early 1990s, NASA was planning for an extended stay on Mars, and Bubenheim and his Ames colleagues were concentrating efforts on creating a complete ecological system to sustain human crewmembers during their time on the Red Planet. The main barrier to developing such a system, he says, is energy. Mars has no power plants, and a regenerative system requires equipment that runs on electricity to do everything from regulating humidity in the atmosphere to monitoring the quality of recycled water. The Ames group started looking at how to best make power on a planet that is millions of miles away from Earth and turned to a hybrid concept combining wind and solar power technologies. The reason was that Mars experiences frequent dust storms that can block nearly all sunlight. When there's a dust storm and the wind is blowing, the wind system could be the dominant power source. When the wind is not blowing and the sun is out, photovoltaics could be the dominant source, says Bubenheim.To develop and test the wind power technology, Ames turned to a remote, harsh environment here on Earth: the South Pole. The South Pole was a really good analog for Mars, says Bubenheim. The technology features for going to Mars were the same technology features needed to make something work at the South Pole.Around the same time that NASA started investigating energy technologies for the Red Planet, the National Science Foundation (NSF) was working on a redesign of their station at the South Pole. To power its operations, NSF used fuel that it flew to the remote location, but the Foundation recognized the benefits of also using onsite renewable energy technologies. In the winter they have small crews and their power requirements are less, says Bubenheim. In the summers, they bring in larger groups and photovoltaics could supply a lot of power. Using renewable energy technology could be a way of reducing the amount of fuel they have to fly in.Technology Transfer To advance wind turbine technology to meet the requirements of extremely harsh environments like that on Mars, Ames partnered with NSF and the Department of Energy. It was clear that a lot of the same features were also desirable for the cold regions of the Earth, says Bubenheim. NASA took the leadership on the team because we had the longest-term technology a Mars turbine.

Mars Technologies↗

Accounting for Herbaceous Communities in Process-Based Models Will Advance Our Understanding of “Grassy” Ecosystems

Grassland and other herbaceous communities cover significant portions of Earth's terrestrial surface and provide many critical services, such as carbon sequestration, wildlife habitat, and food production. Forecasts of global change impacts on these services will require predictive tools, such as process-based dynamic vegetation models. Yet, model representation of herbaceous communities and ecosystems lags substantially behind that of tree communities and forests. The limited representation of herbaceous communities within models arises from two important knowledge gaps: first, our empirical understanding of the principles governing herbaceous vegetation dynamics is either incomplete or does not provide mechanistic information necessary to drive herbaceous community processes with models; second, current model structure and parameterization of grass and other herbaceous plant functional types limits the ability of models to predict outcomes of competition and growth for herbaceous vegetation. In this review, we provide direction for addressing these gaps by: (1) presenting a brief history of how vegetation dynamics have been developed and incorporated into earth system models, (2) reporting on a model simulation activity to evaluate current model capability to represent herbaceous vegetation dynamics and ecosystem function, and (3) detailing several ecological properties and phenomena that should be a focus for both empiricists and modelers to improve representation of herbaceous vegetation in models. Together, empiricists and modelers can improve representation of herbaceous ecosystem processes within models. In so doing, we will greatly enhance our ability to forecast future states of the earth system, which is of high importance given the rapid rate of environmental change on our planet.

biogeochemistry↗

Advanced life support systems in lunar and Martian environments utilizing a higher plant based engineering paradigm

The paper describes a higher-plant-based engineering paradigm for advanced life support in a Controlled Ecological Life Support System (CELSS) on the surface of the moon or Mars, called the CELSS Breadboard Project, designed at John F. Kennedy Space Center. Such a higher-plant-based system would use the plants for a direct food source, gas exchange, water reclamation, and plant residuals in a complex biological resource recovery scheme. The CELSS Breadboard Project utilizes a 'breadboard' approach of developing independent systems that are evaluated autonomously and are later interconnected. Such a scheme will enable evaluation of life support system methodologies tested for their efficiency in a life support system for habitats on the moon or Mars.

Chamberland, Dennis↗

Fisher's Peak Ecological Forecasting: Mapping Biomass to Inform Conservation Planning of a Future State Park in Southern Colorado

Fisher’s Peak is a 77.5 km2 property southeast of Trinidad, Colorado that is planned to become Colorado’s newest state park. The area has experienced limited anthropogenic disturbance and is home to an abundance of unique habitats and species. A rapid, approximately 900 m change in elevation over the extent of the area nurtures a variety of plants and animals, including the endangered New Mexico meadow jumping mouse. In 2019, the State of Colorado obtained Fisher’s Peak with plans to make it Colorado’s second largest state park. A diverse group of collaborators, including the Colorado State Forest Service and The Nature Conservancy, worked closely to design the state park to maximize recreation opportunity while conserving the property’s rich habitats and biodiversity. The Fisher’s Peak Ecological Forecasting Team utilized Light Detection and Ranging (LiDAR) surveys, in situ forest inventory data, and Earth observations from Landsat8 Operational Land Imager (OLI), Sentinel-1 C-band Synthetic Aperture Radar (C-SAR), Sentinel-2 Multispectral Instrument (MSI), Advanced Land Observing Satellite 2 (ALOS-2) Phased Array type L-band Synthetic Aperture Radar (PALSAR-2) and the Shuttle Radar Topography Mission (SRTM) to quantify and map biomass over the extent of the study area. The results from modeling biomass had an out-of-bag root mean square error of 55 Mg/ha and an R2 of 12. The resulting map indicates areas where carbon storage on the property is high, informing decision-making processes for future park development. While more in situ training data may improve modeling capacity for biomass in the Fisher’s Peak area, this work represents a feasible attempt to better understand biomass distribution using earth observation.

DEVELOP Tech Paper↗

Fisher's Peak Ecological Forecasting - Mapping Biomass to Inform Conservation Planning of a Future State Park in Southern Colorado

Fisher’s Peak is a 77.5 km2 property southeast of Trinidad, Colorado that is planned to become Colorado’s newest state park. The area has experienced limited anthropogenic disturbance and is home to an abundance of unique habitats and species. A rapid, approximately 900 m change in elevation over the extent of the area nurtures a variety of plants and animals, including the endangered New Mexico meadow jumping mouse. In 2019, the State of Colorado obtained Fisher’s Peak with plans to make it Colorado’s second largest state park. A diverse group of collaborators, including the Colorado State Forest Service and The Nature Conservancy, worked closely to design the state park to maximize recreation opportunity while conserving the property’s rich habitats and biodiversity. The Fisher’s Peak Ecological Forecasting Team utilized Light Detection and Ranging (LiDAR) surveys, in situ forest inventory data, and Earth observations from Landsat 8 Operational Land Imager (OLI), Sentinel-1 C-band Synthetic Aperture Radar (C-SAR), Sentinel-2 Multispectral Instrument (MSI), Advanced Land Observing Satellite 2 (ALOS-2) Phased Array type L-band Synthetic Aperture Radar (PALSAR-2) and the Shuttle Radar Topography Mission (SRTM) to quantify and map biomass over the extent of the study area. The results from modeling biomass had an out-of-bag root mean square error of 55 Mg/ha and an R2 of 12. The resulting map indicates areas where carbon storage on the property is high, informing decision-making processes for future park development. While more in situ training data may improve modeling capacity for biomass in the Fisher’s Peak area, this work represents a feasible attempt to better understand biomass distribution using earth observation

Lauren Lad↗

Carolina Coastal Plain Ecological Forecasting: Utilizing NASA Earth Observations to Map Suitable Venus Flytrap Habitat in an Effort to Inform Conservation, Seed Banking, and Reintroduction in the Carolina Coastal Plain and Sandhills Regions

Although the carnivorous plant Venus flytrap (Dionaea muscipula) is recognized globally, its native range is restricted to a small portion of the North and South Carolina Coastal Plain and Sandhills. Within this limited range, Venus flytrap populations are threatened by habitat loss, fire suppression, and poaching. NASA DEVELOP partnered with the North Carolina Botanical Garden (NCBG), the University of North Carolina Herbarium (NCU), and the North Carolina Natural Heritage Program (NCNHP) to support Venus flytrap conservation. The team developed models based on species presence data and environmental variables using the Software for Assisted Habitat Modeling (SAHM) to create a 2021 habitat suitability map for Venus flytrap. These models incorporated Earth observations collected by Landsat 8 Thermal Infrared Sensor (TIRS), Terra Moderate Resolution Imaging Spectroradiometer (MODIS), Advanced Land Observation Satellite (ALOS) Phased Array type L-band Synthetic Aperture Radar (PALSAR), and Sentinel-2 Multispectral Instrument (MSI). To predict areas at high risk of development, the team produced a 2050 land-use change map using TerrSet Land Change Modeler. The team found potential areas of conflict between predicted habitat and forecasted future development. Many areas of suitable habitat were concentrated along the coast where development was likely to occur, placing populations there at risk of extirpation. Overlaying suitable habitat with forecasted land change also identified suitable habitats with minimal risk of development, which may serve as lasting Venus flytrap habitat. These results can inform the NCBG and NCNHP’s conservation decision-making, including targeted seed banking, reintroduction, and prioritization of enduring habitats for protection and management.

Monika Rock↗

Carolina Coastal Plain​ Ecological Forecasting

Although the carnivorous plant Venus flytrap (Dionaea muscipula) is recognized globally, its native range is restricted to a small portion of the North and South Carolina Coastal Plain and Sandhills. Within this limited range, Venus flytrap populations are threatened by habitat loss, fire suppression, and poaching. NASA DEVELOP partnered with the North Carolina Botanical Garden (NCBG), the University of North Carolina Herbarium (NCU), and the North Carolina Natural Heritage Program (NCNHP) to support Venus flytrap conservation. The team developed models based on species presence data and environmental variables using the Software for Assisted Habitat Modeling (SAHM) to create a 2021 habitat suitability map for Venus flytrap. These models incorporated Earth observations collected by Landsat 8 Thermal Infrared Sensor (TIRS), Terra Moderate Resolution Imaging Spectroradiometer (MODIS), Advanced Land Observation Satellite (ALOS) Phased Array type L-band Synthetic Aperture Radar (PALSAR), and Sentinel-2 Multispectral Instrument (MSI). To predict areas at high risk of development, the team produced a 2050 land-use change map using TerrSet Land Change Modeler. The team found potential areas of conflict between predicted habitat and forecasted future development. Many areas of suitable habitat were concentrated along the coast where development was likely to occur, placing populations there at risk of extirpation. Overlaying suitable habitat with forecasted land change also identified suitable habitats with minimal risk of development, which may serve as lasting Venus flytrap habitat. These results can inform the NCBG and NCNHP’s conservation decision-making, including targeted seed banking, reintroduction, and prioritization of enduring habitats for protection and management.

Monika Rock↗

Life Sciences Research in the Centrifuge Accommodation Module of the International Space Station

The Centrifuge Accommodation Module (CAM) will be the home of the fundamental biology research facilities on the International Space Station (ISS). These facilities are being built by the Biological Research Project (BRP), whose goal is to oversee development of a wide variety of habitats and host systems to support life sciences research on the ISS. The habitats and host systems are designed to provide life support for a variety of specimens including cells, bacteria, yeast, plants, fish, rodents, eggs (e.g., quail), and insects. Each habitat contains specimen chambers that allow for easy manipulation of specimens and alteration of sample numbers. All habitats are capable of sustaining life support for 90 days and have automated as well as full telescience capabilities for sending habitat parameters data to investigator homesite laboratories. The habitats provide all basic life support capabilities including temperature control, humidity monitoring and control, waste management, food, media and water delivery as well as adjustable lighting. All habitats will have either an internal centrifuge or are fitted to the 2.5-meter diameter centrifuge allowing for variable centrifugation up to 2 g. Specimen chambers are removable so that the specimens can be handled in the life sciences glovebox. Laboratory support equipment is provided for handling the specimens. This includes a compound and dissecting microscope with advanced video imaging, mass measuring devices, refrigerated centrifuge for processing biological samples, pH meter, fixation and complete cryogenic storage capabilities. The research capabilities provided by the fundamental biology facilities will allow for flexibility and efficiency for long term research on the International Space Station.

Dalton, Bonnie P.↗

Designing Payload and Spaceflight Operations for Plants From Extreme 1 Terrestrial Environments

Terrestrial plants from the edges of the limits of life are likely to harbor genes that confer an advantage in deep space environments. These plants are seemingly capable of performing mission critical functions under prevailing deep space conditions while informing directed gene manipulation in target plant species. However, their adaptations to physiologically extreme habitats may hinder efficacy of routine laboratory techniques established for model plants. Here we present the development of Antarctic moss Ceratodon purpureus payload and flight operations for the ARTEMOSS experiment to the ISS astute of limited physical space and crew time. We demonstrate that the hydrophobic surface of Antarctic moss impedes chemical tissue fixation and precludes usage of RNAlater coupled with payload hardware deployed in standard plant spaceflight experiments. We show that deep-freezing the moss tissue on Petri plates provides adequate tissue fixation and allows for extraction of high-quality RNA suitable for gene expression profiling. We replaced hardware with stacks of Petri plates housing Antarctic moss and chemical fixation with deep-freeze in cryogenic GLACIER freezer. Our design can be translated to other plant species, expanding current techniques of experimentation with plants from extreme terrestrial environments aimed toward advancing human space exploration.

Agata K Zupanska↗

A Lunar Ground Truth of Microbes that are Integral to Sustaining Biological Life Support Systems

A lunar outpost such as Gateway or a Mars transit vehicle will use a semi-closed to closed loop biological life support system (BLSS) adapted for microgravity and high levels of radiation. A moon habitat will likely exist under partial gravity conditions and sustained levels of high radiation as a semi-closed loop system able to get resources from Earth via Gateway. Additionally, the moon will act as a closed-loop testbed for Mars habitat operations and will employ various elements of in situ resource utilization (ISRU). Perhaps the longest running BLSS collective study to date is that of the European MELiSSA (Micro Ecological Life Support System Alternative) project, a circular life support system, established to gain knowledge on regenerative systems aimed at the highest degree of autonomy to produce food, water and oxygen from mission wastes. This setup has evaluated the use of discrete microbial compartments and a higher plant compartment to carry out the necessary life support functions to support crew. Such compartments utilize thermophilic anaerobes to break down human and inedible plant wastes, photoheterotrophic bacteria that can further metabolize volatile fatty acids, nitrifying bacteria that can convert ammonium to plant and microalgae available nitrates, and photoautotrophic bacteria and higher plants which will convert carbon dioxide to oxygen, purify water, and provide food for human consumption. This being the most advanced BLSS model example to date -aside from the Yuegong-1, Chinese Lunar Palace- with the most defined compartment composition, and the assumption that other BLSSs will build upon such models, it becomes relevant to study the effects of the lunar environment upon the microbes that are integral to a BLSS. In ramping up to advanced stage BLSS systems, relatively simple experiments can be conducted on Artemis missions using continuous culturing and sampling of the model microbes, subsequently subjected to DNA sequencing for mutational analysis monitoring and chemical analysis to assess the sustained ability to carry out their hallmark biochemical processes efficiently. A suite of microbes should be assessed, prioritizing those with the duel capacity to be utilized in an BLSS and the ability to biochemically facilitate ISRU goals, such as the ability to transform the biogeochemistry of Moon or Martian regolith into materials that can support crop growth or extract elements of industrial significance such as aluminum or iron.

Biological Life Support Systems↗

Long -Term Architecture Development For The Moon And M

Developing an enterprise-level architecture encompassing future human-robotic science and exploration at the Moon and Mars requires a visionary approach that ensures NASA is responsive to national priorities and global science and technology advancement objectives. While the initial capabilities needed to return humans to the Moon may be well understood, NASA is still formulating a long-term infrastructure at the Moon and working to narrow the trade space for the first human missions to Mars. This paper will focus on the formulation and current status of engineering and design applications for a long-term and robust lunar architecture that will implement the Artemis Base Camp concept, including pre-formulation activities and elements currently in formulation. Authors will demonstrate how the Moon-to-Mars campaign approach fosters commonality of requirements and standards across individual mission elements, as well as multi-destination systems and operations, to reduce risk and encourage healthy competition in the growing space market. Among the Artemis Base Camp elements discussed will be multiple surface mobility elements, a fixed, anchoring surface habitat, fission surface power, and an in-situ resource development pilot plant. The narrative will clearly illustrate the direct evolution of the base camp elements to a minimal Mars architecture concept, and also outline unique development efforts that will be required in the next decade to make it possible to send humans to the Red Planet as early as the 2030s.

Greg Chavers↗

Flowering Date of Taxonomic Families Predicts Phenological Sensitivity to Temperature: Implications for Forecasting the Effects of Climate Change on Unstudied Taxa

Premise of the study: Numerous long-term studies in seasonal habitats have tracked interannual variation in fi rst fl owering date (FFD) in relation to climate, documenting the effect of warming on the FFD of many species. Despite these efforts, long-term phenological observations are still lacking for many species. If we could forecast responses based on taxonomic affi nity, however, then we could leverage existing data to predict the climate-related phenological shifts of many taxa not yet studied; Methods: We examined phenological time series of 1226 species occurrences (1031 unique species in 119 families) across seven sites in North America and England to determine whether family membership (or family mean FFD) predicts the sensitivity of FFD to standardized interannual changes in temperature and precipitation during seasonal periods before fl owering and whether families differ signifi cantly in the direction of their phenological shifts; Key results: Patterns observed among species within and across sites are mirrored among family means across sites; earlyfl owering families advance their FFD in response to warming more than late-fl owering families. By contrast, we found no consistent relationships among taxa between mean FFD and sensitivity to precipitation as measured here; Conclusions: Family membership can be used to identify taxa of high and low sensitivity to temperature within the seasonal, temperate zone plant communities analyzed here. The high sensitivity of early-fl owering families (and the absence of earlyfl owering families not sensitive to temperature) may refl ect plasticity in fl owering time, which may be adaptive in environments where early-season conditions are highly variable among years.

first flowering date↗

Climate Change Adaptation Activities at the NASA John F. Kennedy Space Center, FL., USA

In 2010, the Office of Strategic Infrastructure and Earth Sciences established the Climate Adaptation Science Investigators (CASI) program to integrate climate change forecasts and knowledge into sustainable management of infrastructure and operations needed for the NASA mission. NASA operates 10 field centers valued at $32 billion dollars, occupies 191,000 acres and employs 58,000 people. CASI climate change and sea-level rise forecasts focus on the 2050 and 2080 time periods. At the 140,000 acre Kennedy Space Center (KSC) data are used to simulate impacts on infrastructure, operations, and unique natural resources. KSC launch and processing facilities represent a valued national asset located in an area with high biodiversity including 33 species of special management concern. Numerical and advanced Bayesian and Monte Carlo statistical modeling is being conducted using LiDAR digital elevation models coupled with relevant GIS layers to assess potential future conditions. Results are provided to the Environmental Management Branch, Master Planning, Construction of Facilities, Engineering Construction Innovation Committee and our regional partners to support Spaceport development, management, and adaptation planning and design. Potential impacts to natural resources include conversion of 50% of the Center to open water, elevation of the surficial aquifer, alterations of rainfall and evapotranspiration patterns, conversion of salt marsh to mangrove forest, reductions in distribution and extent of upland habitats, overwash of the barrier island dune system, increases in heat stress days, and releases of chemicals from legacy contamination sites. CASI has proven successful in bringing climate change planning to KSC including recognition of the need to increase resiliency and development of a green managed shoreline retreat approach to maintain coastal ecosystem services while maximizing life expectancy of Center launch and payload processing resources.

plant communities↗