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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 91 records · Page 5
All sourcesNASA NTRSDOE OSTI
DOE OSTI2023

Evaluation of 1,2-diacyl-3-acetyl triacylglycerol production in Yarrowia lipolytica

Plants produce many high-value oleochemical molecules. While oil-crop agriculture is performed at industrial scales, suitable land is not available to meet global oleochemical demand. Worse, establishing new oil-crop farms often comes with the environmental cost of tropical deforestation. The field of metabolic engineering offers tools to transplant oleochemical metabolism into tractable hosts while simultaneously providing access to molecules produced by non-agricultural plants. Here, we evaluate strategies for rewiring metabolism in the oleaginous yeast Yarrowia lipolytica to synthesize a foreign lipid, 3-acetyl-1,2-diacyl-sn-glycerol (acTAG). Oils made up of acTAG have a reduced viscosity and melting point relative to traditional triacylglycerol oils making them attractive as low-grade diesels, lubricants, and emulsifiers. Furthermore, this manuscript describes a metabolic engineering study that established acTAG production at g/L scale, exploration of the impact of lipid bodies on acTAG titer, and a techno-economic analysis that establishes the performance benchmarks required for microbial acTAG production to be economically feasible.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2023

Data for Evaluation of 1,2-Diacyl-3-Acetyl Triacylglycerol Production in Yarrowia lipolytica

Plants produce many high-value oleochemical molecules. While oil-crop agriculture is performed at industrial scales, suitable land is not available to meet global oleochemical demand. Worse, establishing new oil-crop farms often comes with the environmental cost of tropical deforestation. The field of metabolic engineering offers tools to transplant oleochemical metabolism into tractable hosts while simultaneously providing access to molecules produced by non-agricultural plants. Here, we evaluate strategies for rewiring metabolism in the oleaginous yeast Yarrowia lipolytica to synthesize a foreign lipid, 3-acetyl-1,2-diacyl-sn-glycerol (acTAG). Oils made up of acTAG have a reduced viscosity and melting point relative to traditional triacylglycerol oils making them attractive as low-grade diesels, lubricants, and emulsifiers. This manuscript describes a metabolic engineering study that established acTAG production at g/L scale, exploration of the impact of lipid bodies on acTAG titer, and a techno-economic analysis that establishes the performance benchmarks required for microbial acTAG production to be economically feasible.

Biomass Analytics↗
DOE OSTI2024

Quantitative phenotyping of crop roots with spectral electrical impedance tomography: a rhizotron study with optimized measurement design

Background: Root systems are key contributors to plant health, resilience, and, ultimately, yield of agricultural crops. To optimize plant performance, phenotyping trials are conducted to breed plants with diverse root traits. However, traditional analysis methods are often labour-intensive and invasive to the root system, therefore limiting high-throughput phenotyping. Spectral electrical impedance tomography (sEIT) could help as a non-invasive and cost-efficient alternative to optical root analysis, potentially providing 2D or 3D spatio-temporal information on root development and activity. Although impedance measurements have been shown to be sensitive to root biomass, nutrient status, and diurnal activity, only few attempts have been made to employ tomographic algorithms to recover spatially resolved information on root systems. In this study, we aim to establish relationships between tomographic electrical polarization signatures and root traits of different fine root systems (maize, pinto bean, black bean, and soy bean) under hydroponic conditions. Results: Our results show that, with the use of an optimized data acquisition scheme, sEIT is capable of providing spatially resolved information on root biomass and root surface area for all investigated root systems. We found strong correlations between the total polarization strength and the root biomass (R 2 = 0.82) and root surface area (R 2 = 0.8). Our findings suggest that the captured polarization signature is dominated by cell-scale polarization processes. Additionally, we demonstrate that the resolution characteristics of the measurement scheme can have a significant impact on the tomographic reconstruction of root traits. Conclusion: Our findings showcase that sEIT is a promising tool for the tomographic reconstruction of root traits in high-throughput root phenotyping trials and should be evaluated as a substitute for traditional, often time-consuming, root characterization methods.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS1980

Crop identification studies using Landsat data Separation of barley from other spring small grains and corn and soybean decision logic

Two labeling procedures were developed which identify various agricultural crops through the use of Landsat data. One procedure separates barley from other spring small grains, and the other identifies corn and soybeans. For both procedures, a minimum data set (critical acquisition time) has been designated. Landsat data in both image format and various graphic displays were used along with ancillary data to obtain information which aided in labeling the spectral signatures. The corn and soybean procedure also employed a structured decision logic. Test results for the barley separation procedure emphasized the importance of obtaining a critical acquisition and showed some success especially in areas where spring crops followed the expected growth patterns. Two tests of the corn and soybean procedure produced good labeling accuracies. Problems with the procedure were easy to identify, and some solutions were implemented for the second test. Automation of various parts of the procedure and extension to other crops and regions were recommended.

Dailey, C. L.↗
NASA NTRS1975

Agricultural inventory capabilities of machine processed LANDSAT digital data

Agricultural crop identification and acreage determination analysis of LANDSAT digital data was performed for two study areas. A multispectral image processing and analysis system was utilized to perform the manmachine interactive analysis. The developed techniques yielded crop acreage estimate results with accuracy greater than 90% and as high as 99%. These results are encouraging evidence of agricultural inventory capabilities of machine processed LANDSAT digital data.

Dietrick, D. L.↗
DOE OSTI2024

Nitrogen-Fixing Gamma Proteobacteria Azotobacter vinelandii—A Blueprint for Nitrogen-Fixing Plants?

The availability of fixed nitrogen limits overall agricultural crop production worldwide. The so-called modern “green revolution” catalyzed by the widespread application of nitrogenous fertilizer has propelled global population growth. It has led to imbalances in global biogeochemical nitrogen cycling, resulting in a “nitrogen problem” that is growing at a similar trajectory to the “carbon problem”. As a result of the increasing imbalances in nitrogen cycling and additional environmental problems such as soil acidification, there is renewed and increasing interest in increasing the contributions of biological nitrogen fixation to reduce the inputs of nitrogenous fertilizers in agriculture. Interestingly, biological nitrogen fixation, or life’s ability to convert atmospheric dinitrogen to ammonia, is restricted to microbial life and not associated with any known eukaryotes. It is not clear why plants never evolved the ability to fix nitrogen and rather form associations with nitrogen-fixing microorganisms. Perhaps it is because of the large energy demand of the process, the oxygen sensitivity of the enzymatic apparatus, or simply failure to encounter the appropriate selective pressure. Whatever the reason, it is clear that this ability of crop plants, especially cereals, would transform modern agriculture once again. Successfully engineering plants will require creating an oxygen-free niche that can supply ample energy in a tightly regulated manner to minimize energy waste and ensure the ammonia produced is assimilated. Nitrogen-fixing aerobic bacteria can perhaps provide a blueprint for engineering nitrogen-fixing plants. This short review discusses the key features of robust nitrogen fixation in the model nitrogen-fixing aerobe, gamma proteobacteria Azotobacter vinelandii, in the context of the basic requirements for engineering nitrogen-fixing plants.

Microbiology↗
NASA NTRS2019

2018 NISAR Applications Workshop: Agriculture and Soil Moisture

Agricultural lands cover the globe and play an essential role in not only sustaining a growing global population, but can have significant implications on the Earth system through land use change (e.g., deforestation, grazing, etc.). As such, countries around the world have dedicated programs for managing these lands. Accurate and timely information concerning the status of agricultural crops (soil moisture, crop health, crop type, etc.) is essential to those nations’ anthropogenic and ecological health as well as economy. The joint NASA/US Department of Agriculture Agricultural Research Service (USDA-ARS) workshop focused on advancing agriculture and soil moisture applications by using remote sensing data from the NASA-ISRO Synthetic Aperture Radar (NISAR) mission (expected launch 2022). Participants included representatives from the international agriculture community that are key players in facilitating integration of Earth Observations into decision support workflows including US Federal Agencies, nonprofits, and private sector. They included scientists, technicians, and program managers with a responsibility for data acquisition and exploitation such as product development, delivery, and use, as well as capacity building. Discussions were held over two and a half days to convey the broader agriculture and soil moisture community information needs, the mission and procedures for various representative participants and programs involved in the delivery of geospatial products, and the capabilities and status of the NISAR mission. Case studies were presented to demonstrate the current state of practice in the use of SAR remote sensing for applications of direct importance for the agriculture and soil moisture communities. Eleven organizations presented their information requirements in response to a set of questions provided by the NASA team, then the NASA team responded by describing the degree to which NISAR could meet these requirements. Discussion ensued about needed data product specifications to increase utility (e.g., projection, latency, etc.), tools and capacity building.

Stavros, Natasha↗
DOE OSTI2022

Feedstock Carbon Intensity Calculator (FD-CIC): Users’ Manual and Technical Documentation

The carbon intensities (CIs) of biofuels are determined with the life cycle analysis (LCA) technique, which accounts for the energy/material uses and emissions during the complete supply chain of biofuel including feedstock production and fuel conversion stages. Regulatory agencies such as California Air Resources Board (CARB) adopts LCA to calculate biofuel CIs. The Low Carbon Fuel Standard (LCFS) program developed by CARB allows individual biofuel conversion facilities to submit their own biofuel CIs with their facility input data and incentivizes the reduction in the CI specific to that particular facility compared to a reference fuel’s CI (Liu et al 2020). Such an incentive program has driven innovations in biorefineries to reduce their greenhouse gas (GHG) emissions by linking their revenue directly to its CI score through LCFS credit trading. Besides the biofuel conversion stage, different farming practices for feedstock growth can result in significant CI variations for feedstocks, thus for biofuels. To provide evidence-based research findings, the U.S. Department of Energy’s Advanced Research Projects Agency–Energy (ARPA-E) has supported the Systems Assessment Center of the Energy Systems and Infrastructure Analysis Division at Argonne National Laboratory to examine CI variations of different farming practices to grow agricultural crops for biofuel production. Meanwhile, the ARPA-E has launched the Systems for Monitoring and Analytics for Renewable Transportation Fuels from Agricultural Resources and Management (SMARTFARM) program to develop technologies and data platforms that enable an accurate measurement of key farming parameters that can help robust accounting of the GHG benefits of sustainable, low-carbon agronomic practices at farm level.

54 ENVIRONMENTAL SCIENCES↗
NASA NTRS1985

Space Chambers for Crop Treatment

Vacuum chambers, operated by McDonnell Douglas Corporation to test spacecraft, can also be used to dry water-soaked records. The drying temperature is low enough to allow paper to dry without curling or charging. Agricultural crops may also be dried using a spinoff system called MIVAC, which has proven effective in drying rice, wheat, soybeans, corn, etc. The system is energy efficient and can incorporate a sanitation process for destroying insects without contamination.

Source record↗
NASA NTRS1984

Thematic Mapper Spectral Dimensionality and Data Structure

Thematic Mapper data, simulated from field and laboratory spectrometer measurements of a variety of agricultural crops and a wide range of soils, are analyzed to determine their dispersion in the six space defined by the reflective TM bands (i.e., excluding the thermal band). While similar analyses of MSS data from agricultural scenes show that the vast majority of the MSS data occupy a single plane, the simulated TM data primarily occupy three dimensions, defining two intersecting planes and a zone of transition between the two. Viewing the plane of Vegetation head on provides a projection comparable to the single plane of MSS data. The Plane of Soils and transition zone represent new information made available largely as a result of the longer infrared bands included in the Thematic Mapper. A transformation, named the Thematic Mapper Tasseled Cap, is presented which rotates the TM data such that the described data structure is most readily accessible to view.

Crist, E. P.↗
NASA NTRS2022

Satellite-Based Characterization of Convection and Impacts from the Catastrophic 10 August 2020 Midwest U.S. Derecho

The catastrophic derecho that occurred on 10 August 2020 across the Midwest United States caused billions of dollars of damage to both urban and rural infrastructure as well as agricultural crops, most notably across the state of Iowa. This paper documents the complex evolution of the derecho through the use of low-Earth orbit passive-microwave imager and GOES-16satellite-derived products complemented by products derived from NEXRAD weather radar observations. Additional satellite sensors including optical imagers and synthetic aperture radar (SAR) were used to observe impacts to the power grid and agriculture in Iowa. SAR improved the identification and quantification of damaged corn and soybeans, as compared to true-color composites and Normalized Difference Vegetation Index (NDVI). A statistical approach to identify damaged corn and soybean crops from SAR was created with estimates of 1.97 million acres of damaged corn and 1.40 million acres of damaged soybeans in the state of Iowa. The damage estimates generated by this study were comparable to estimates produced by others after the derecho, including two commercial agricultural companies.

Derecho↗
DOE OSTI2020

Distinct Preflowering Drought Tolerance Strategies of Sorghum bicolor Genotype RTx430 Revealed by Subcellular Protein Profiling

Drought is the largest stress affecting agricultural crops, resulting in substantial reductions in yield. Plant adaptation to water stress is a complex trait involving changes in hormone signaling, physiology, and morphology. Sorghum (Sorghum bicolor (L.) Moench) is a C4 cereal grass; it is an agricultural staple, and it is particularly drought-tolerant. To better understand drought adaptation strategies, we compared the cytosolic- and organelle-enriched protein profiles of leaves from two Sorghum bicolor genotypes, RTx430 and BTx642, with differing preflowering drought tolerances after 8 weeks of growth under water limitation in the field. In agreement with previous findings, we observed significant drought-induced changes in the abundance of multiple heat shock proteins and dehydrins in both genotypes. Interestingly, our data suggest a larger genotype-specific drought response in protein profiles of organelles, while cytosolic responses are largely similar between genotypes. Organelle-enriched proteins whose abundance significantly changed exclusively in the preflowering drought-tolerant genotype RTx430 upon drought stress suggest multiple mechanisms of drought tolerance. These include an RTx430-specific change in proteins associated with ABA metabolism and signal transduction, Rubisco activation, reactive oxygen species scavenging, flowering time regulation, and epicuticular wax production. We discuss the current understanding of these processes in relation to drought tolerance and their potential implications.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS1975

Plan for the uniform mapping of earth resources and environmental complexes from Skylab imagery. Assessment of natural vegetation, environmental, and crop analogs

The author has identified the following significant results. For interpreting a wide range of natural vegetation analogs, S-190A color infrared and the ERTS-1 color composite were consistently more useful than were conventional color or black and white photos. Color infrared was superior for five vegetation analogs while color was superior for only three. The errors in identification appeared to associate more with black and white single band images than with multiband color. For rice crop analogs, spectral and spatial discriminations both contribute to the usefulness of images for data collection. Tests and subjective analyses conducted in this study indicated that the spectral bands exploited in color infrared film were the most useful for agricultural crop analysis. Accuracy of crop identification on any single date of Skylab images was less than that of multidate analysis due to differences in crop calendar, cultural practices used, rice variety, planting date, planting method, water use, fertilization, disease, or mechanical problems.

Poulton, C. E.↗
NASA NTRS1983

International Geoscience and Remote Sensing Symposium, Universitaet Muenchen, Munich, West Germany, June 1-4, 1982, Proceedings

The present conference on geoscience and remote sensing considers the multispectral remote sensing of saline seeps, the augmentation of LANDSAT MSS data with topographic data, thematic mapping, the sampling problem in radiation budget studies, aerial conductivity measurements over geothermal areas, a comparison of multifrequency band radars for crop classification, the improved estimation of vegetation-covered soil by combined active/passive microwave remote sensing, and atmospheric water vapor profiling by ground-based radiometry. Also discussed are SAR imaging from an inclined geosynchronous orbit, the classification of agricultural crops in radar images, the Ocean Color Experiment on the second orbital flight test of the Space Shuttle, the dielectric properties of wet materials, remote sensing systems for the mm-wave region, and the simulation of spaceborne stereo radar imagery.

Seiber, A. J.↗
DOE OSTI2023

Spray‐induced gene silencing to identify powdery mildew gene targets and processes for powdery mildew control

Abstract Spray‐induced gene silencing (SIGS) is an emerging tool for crop pest protection. It utilizes exogenously applied double‐stranded RNA to specifically reduce pest target gene expression using endogenous RNA interference machinery. In this study, SIGS methods were developed and optimized for powdery mildew fungi, which are widespread obligate biotrophic fungi that infect agricultural crops, using the known azole‐fungicide target cytochrome P450 51 (CYP51) in the Golovinomyces orontii–Arabidopsis thaliana pathosystem. Additional screening resulted in the identification of conserved gene targets and processes important to powdery mildew proliferation: apoptosis‐antagonizing transcription factor in essential cellular metabolism and stress response; lipid catabolism genes lipase a , lipase 1 , and acetyl‐CoA oxidase in energy production ; and genes involved in manipulation of the plant host via abscisic acid metabolism ( 9‐cis‐epoxycarotenoid dioxygenase , xanthoxin dehydrogenase , and a putative abscisic acid G‐protein coupled receptor ) and secretion of the effector protein, effector candidate 2 . Powdery mildew is the dominant disease impacting grapes and extensive powdery mildew resistance to applied fungicides has been reported. We therefore developed SIGS for the Erysiphe necator–Vitis vinifera system and tested six successful targets identified using the G. orontii–A. thaliana system. For all targets tested, a similar reduction in powdery mildew disease was observed between systems. This indicates screening of broadly conserved targets in the G. orontii–A. thaliana pathosystem identifies targets and processes for the successful control of other powdery mildew fungi. The efficacy of SIGS on powdery mildew fungi makes SIGS an exciting prospect for commercial powdery mildew control.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS1985

Crop Characteristics Research: Growth and Reflectance Analysis

Much of the early research in remote sensing follows along developing spectral signatures of cover types. It was found, however, that a signature from an unknown cover class could not always be matched to a catalog value of known cover class. This approach was abandoned and supervised classification schemes followed. These were not efficient and required extensive training. It was obvious that data acquired at a single time could not separate cover types. A large portion of the proposed research has concentrated on modeling the temporal behavior of agricultural crops and on removing the need for any training data in remote sensing surveys; the key to which is the solution of the so-called 'signature extension' problem. A clear need to develop spectral estimaters of crop ontogenic stages and yield has existed even though various correlations have been developed. Considerable effort in developing techniques to estimate these variables was devoted to this work. The need to accurately evaluate existing canopy reflectance model(s), improve these models, use them to understand the crop signatures, and estimate leaf area index was the third objective of the proposed work. A synopsis of this research effort is discussed.

Badhwar, G. D.↗
DOE OSTI2021

Inferring pesticide toxicity to honey bees from a field‐based feeding study using a colony model and Bayesian inference

Abstract Honey bees are crucial pollinators for agricultural crops but are threatened by a multitude of stressors including exposure to pesticides. Linking our understanding of how pesticides affect individual bees to colony‐level responses is challenging because colonies show emergent properties based on complex internal processes and interactions among individual bees. Agent‐based models that simulate honey bee colony dynamics may be a tool for scaling between individual and colony effects of a pesticide. The U.S. Environmental Protection Agency (USEPA) and U.S. Department of Agriculture (USDA) are developing the VarroaPop + Pesticide model, which simulates the dynamics of honey bee colonies and how they respond to multiple stressors, including weather, Varroa mites, and pesticides. To evaluate this model, we used Approximate Bayesian Computation to fit field data from an empirical study where honey bee colonies were fed the insecticide clothianidin. This allowed us to reproduce colony feeding study data by simulating colony demography and mortality from ingestion of contaminated food. We found that VarroaPop + Pesticide was able to fit general trends in colony population size and structure and reproduce colony declines from increasing clothianidin exposure. The model underestimated adverse effects at low exposure (36 µg/kg), however, and overestimated recovery at the highest exposure level (140 µg/kg), for the adult and pupa endpoints, suggesting that mechanisms besides oral toxicity‐induced mortality may have played a role in colony declines. The VarroaPop + Pesticide model estimates an adult oral LD 50 of 18.9 ng/bee (95% CI 10.1–32.6) based on the simulated feeding study data, which falls just above the 95% confidence intervals of values observed in laboratory toxicology studies on individual bees. Overall, our results demonstrate a novel method for analyzing colony‐level data on pesticide effects on bees and making inferences on pesticide toxicity to individual bees.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS1977

Variations of temporal, spectral and angular radar backscattering coefficient of vegetation

Results of an experimental program is reviewed which over the past five years has succeeded in documenting the variations of the radar backscattering coefficient of a variety of crop types as a function of time, signal frequency (1-18 GHz), angular range (nadir to 80 deg) and polarization (HH, VV and HV). The systems were the microwave active spectrometers (MAS); one MAS system covers the 1-8 GHz band and the other system covers the 8-18 GHz band. Each MAS system was mounted on a mobile truck-mounted boom and was operated by a computer controller. To date, these two systems have acquired over 3 million data points from agricultural crops, bare ground and trees.

Ulaby, F. T.↗