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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 91 records · Page 5
All sourcesNASA NTRSDOE OSTI
NASA NTRS2001

Pulse Detonation Engine Modeled

Pulse Detonation Engine Technology is currently being investigated at Glenn for both airbreathing and rocket propulsion applications. The potential for both mechanical simplicity and high efficiency due to the inherent near-constant-volume combustion process, may make Pulse Detonation Engines (PDE's) well suited for a number of mission profiles. Assessment of PDE cycles requires a simulation capability that is both fast and accurate. It should capture the essential physics of the system, yet run at speeds that allow parametric analysis. A quasi-one-dimensional, computational-fluid-dynamics-based simulation has been developed that may meet these requirements. The Euler equations of mass, momentum, and energy have been used along with a single reactive species transport equation, and submodels to account for dominant loss mechanisms (e.g., viscous losses, heat transfer, and valving) to successfully simulate PDE cycles. A high-resolution numerical integration scheme was chosen to capture the discontinuities associated with detonation, and robust boundary condition procedures were incorporated to accommodate flow reversals that may arise during a given cycle. The accompanying graphs compare experimentally measured and computed performance over a range of operating conditions for a particular PDE. Experimental data were supplied by Fred Schauer and Jeff Stutrud from the Air Force Research Laboratory at Wright-Patterson AFB and by Royce Bradley from Innovative Scientific Solutions, Inc. The left graph shows thrust and specific impulse, Isp, as functions of equivalence ratio for a PDE cycle in which the tube is completely filled with a detonable hydrogen/air mixture. The right graph shows thrust and specific impulse as functions of the fraction of the tube that is filled with a stoichiometric mixture of hydrogen and air. For both figures, the operating frequency was 16 Hz. The agreement between measured and computed values is quite good, both in terms of trend and magnitude. The error is under 10 percent everywhere except for the thrust value at an equivalence ratio of 0.8 in the left figure, where it is 14 percent. The simulation results shown were made using 200 numerical cells. Each cycle of the engine, approximately 0.06 sec, required 2.0 min of CPU time on a Sun Ultra2. The simulation is currently being used to analyze existing experiments, design new experiments, and predict performance in propulsion concepts where the PDE is a component (e.g., hybrid engines and combined cycles).

Paxson, Daniel E.↗
NASA NTRS2008

Documentation for Three Wake Vortex Model Data Sets from Simulation of Flight 587 Wake Vortex Encounter Accident Case

This document contains a general description for data sets of a wake vortex system in a turbulent environment. The turbulence and thermal stratification of the environment are representative of the conditions on November 12, 2001 near John F. Kennedy International Airport. The simulation assumes no ambient winds. The full three dimensional simulation of the wake vortex system from a Boeing 747 predicts vortex circulation levels at 80% of their initial value at the time of the proposed vortex encounter. The linked vortex oval orientation showed no twisting, and the oval elevations at the widest point were about 20 meters higher than where the vortex pair joined. Fred Proctor of NASA?s Langley Research Center presented the results from this work at the NTSB public hearing that started 29 October 2002. This document contains a description of each data set including: variables, coordinate system, data format, and sample plots. Also included are instructions on how to read the data.

Switzer, George F.↗
NASA NTRS2003

An Overview of Autogyros and The McDonnell XV-1 Convertiplane

This report and its lengthy appendix first reviews early autogyro history. The period from Juan de la Cierva's invention in the early1920s through to the U. S. Army Air Corps' choice, in 1943, of the helicopter instead of the more fully developed autogyro, is examined from a technical point of view. With this historical background in hand, simple aerodynamic technology for rotors, wings, propeller, and fuselages is provided for reference. The McDonnell XV-1 convertiplane development and its program are discussed in detail, with particular emphasis on the wind tunnel and flight testing that was accomplished with two prototype aircraft in the early 1950s. The tip drive rotor system with its ingeniously designed hub was well suited to high speed rotorcraft. The configuration was conceived by Kurt Hohenemser and Fred Dubloff. Many photographs taken of the XV-1 stored at Fort Rucker are included in this report's appendix.

Harris, Franklin D.↗
NASA NTRS2011

Image Analysis via Soft Computing: Prototype Applications at NASA KSC and Product Commercialization

This slide presentation reviews the use of "soft computing" which differs from "hard computing" in that it is more tolerant of imprecision, partial truth, uncertainty, and approximation and its use in image analysis. Soft computing provides flexible information processing to handle real life ambiguous situations and achieve tractability, robustness low solution cost, and a closer resemblance to human decision making. Several systems are or have been developed: Fuzzy Reasoning Edge Detection (FRED), Fuzzy Reasoning Adaptive Thresholding (FRAT), Image enhancement techniques, and visual/pattern recognition. These systems are compared with examples that show the effectiveness of each. NASA applications that are reviewed are: Real-Time (RT) Anomaly Detection, Real-Time (RT) Moving Debris Detection and the Columbia Investigation. The RT anomaly detection reviewed the case of a damaged cable for the emergency egress system. The use of these techniques is further illustrated in the Columbia investigation with the location and detection of Foam debris. There are several applications in commercial usage: image enhancement, human screening and privacy protection, visual inspection, 3D heart visualization, tumor detections and x ray image enhancement.

Dominguez, Jesus A.↗
NASA NTRS2003

A National Virtual Specimen Database for Early Cancer Detection

Access to biospecimens is essential for enabling cancer biomarker discovery. The National Cancer Institute's (NCI) Early Detection Research Network (EDRN) comprises and integrates a large number of laboratories into a network in order to establish a collaborative scientific environment to discover and validate disease markers. The diversity of both the institutions and the collaborative focus has created the need for establishing cross-disciplinary teams focused on integrating expertise in biomedical research, computational and biostatistics, and computer science. Given the collaborative design of the network, the EDRN needed an informatics infrastructure. The Fred Hutchinson Cancer Research Center, the National Cancer Institute,and NASA's Jet Propulsion Laboratory (JPL) teamed up to build an informatics infrastructure creating a collaborative, science-driven research environment despite the geographic and morphology differences of the information systems that existed within the diverse network. EDRN investigators identified the need to share biospecimen data captured across the country managed in disparate databases. As a result, the informatics team initiated an effort to create a virtual tissue database whereby scientists could search and locate details about specimens located at collaborating laboratories. Each database, however, was locally implemented and integrated into collection processes and methods unique to each institution. This meant that efforts to integrate databases needed to be done in a manner that did not require redesign or re-implementation of existing system

distributed↗
NASA NTRS2012

Rapid Process to Generate Beam Envelopes for Optical System Analysis

The task of evaluating obstructions in the optical throughput of an optical system requires the use of two disciplines, and hence, two models: optical models for the details of optical propagation, and mechanical models for determining the actual structure that exists in the optical system. Previous analysis methods for creating beam envelopes (or cones of light) for use in this obstruction analysis were found to be cumbersome to calculate and take significant time and resources to complete. A new process was developed that takes less time to complete beam envelope analysis, is more accurate and less dependent upon manual node tracking to create the beam envelopes, and eases the burden on the mechanical CAD (computer-aided design) designers to form the beam solids. This algorithm allows rapid generation of beam envelopes for optical system obstruction analysis. Ray trace information is taken from optical design software and used to generate CAD objects that represent the boundary of the beam envelopes for detailed analysis in mechanical CAD software. Matlab is used to call ray trace data from the optical model for all fields and entrance pupil points of interest. These are chosen to be the edge of each space, so that these rays produce the bounding volume for the beam. The x and y global coordinate data is collected on the surface planes of interest, typically an image of the field and entrance pupil internal of the optical system. This x and y coordinate data is then evaluated using a convex hull algorithm, which removes any internal points, which are unnecessary to produce the bounding volume of interest. At this point, tolerances can be applied to expand the size of either the field or aperture, depending on the allocations. Once this minimum set of coordinates on the pupil and field is obtained, a new set of rays is generated between the field plane and aperture plane (or vice-versa). These rays are then evaluated at planes between the aperture and field, at a desired number of steps perceived necessary to build up the bounding volume or cone shape. At each plane, the ray coordinates are again evaluated using the convex hull algorithm to reduce the data to a minimal set. When all of the coordinates of interest are obtained for every plane of the propagation, the data is formatted into an xyz file suitable for FRED optical analysis software to import and create a STEP file of the data. This results in a spiral-like structure that is easily imported by mechanical CAD users who can then use an automated algorithm to wrap a skin around it and create a solid that represents the beam.

Howard, Joseph↗
NASA NTRS2012

The Damper Spring Unit of the Sentinel 1 Solar Array

The Damper Spring Unit (DSU, see Figure 1) has been designed to provide the damping required to control the deployment speed of the spring driven solar array deployment in an ARA Mk3 or FRED based Solar Array in situations where the standard application of a damper at the root-hinge is not feasible. The unit consists of four major parts: a main bracket, an eddy current damper, a spring unit, an actuation pulley which is coupled via Kevlar cables to a synchro-pulley of a hinge. The damper slows down the deployment speed and prevents deployment shocks at deployment completion. The spring unit includes 4 springs which overcome the resistances of the damper and the specific DSU control cable loop. This means it can be added to any spring driven deployment system without major modifications of that system. Engineering models of the Sentinel 1 solar array wing have been built to identify the deployment behavior, and to help to determine the optimal pulley ratios of the solar array and to finalize the DSU design. During the functional tests, the behavior proved to be very sensitive for the alignment of the DSU. This was therefore monitored carefully during the qualification program, especially prior to the TV cold testing. During TV "Cold" testing the measured retarding torque exceeded the max. required value: 284 N-mm versus the required 247 N-mm. Although this requirement was not met, the torque balance analysis shows that the 284 N-mm can be accepted, because the spring unit can provide 1.5 times more torque than required. Some functional tests of the DSU have been performed without the eddy current damper attached. It provided input data for the ADAMS solar array wing model. Simulation of the Sentinel-1 deployment (including DSU) in ADAMS allowed the actual wing deployment tests to be limited in both complexity and number of tests. The DSU for the Sentinel-1 solar array was successfully qualified and the flight models are in production.

Doejaaren, Frans↗
NASA NTRS2016

Optics of Balloon Experimental Twin Telescope for Infrared Interferometry (BETTII): Delay Lines and Alignment

We present the optics of Balloon Experimental Twin Telescope for Infrared Interferometry (BETTII) as it gets ready for launch. BETTII is an 8-meter baseline far-infrared (30-90 microns) interferometer mission with capabilities of spatially resolved spectroscopy aimed at studying star formation and galaxy evolution. The instrument collects light from its two arms, makes them interfere, divides them into two science channels (30-50 microns and 60-90 microns), and focuses them onto the detectors. It also separates out the NIR light (1-2.5 microns) and uses it for tip-tilt corrections of the telescope pointing. Currently, all the optical elements have been fabricated, heat treated, coated appropriately and are mounted on their respective assemblies. We are presenting the optical design challenges for such a balloon borne spatio-spectral interferometer, and discuss how they have been mitigated. The warm and cold delay lines are an important part of this optics train. The warm delay line corrects for path length differences between the left and the right arm due to balloon pendulation, while the cold delay line is aimed at introducing a systematic path length difference, thereby generating our interferograms from where we can derive information about the spectra. The details of their design and the results of the testing of these opto-mechanical parts are also discussed. The sensitivities of different optical elements on the interferograms produced have been determined with the help of simulations using FRED software package. Accordingly, an alignment plan is drawn up which makes use of a laser tracker, a CMM, theodolites and a LUPI interferometer.

Dhabal, Arnab↗
NASA NTRS2026

Assessment of Near-Angle Scatter on Exo-Earth Coronagraphy

Near-Angle Scatter (NAS) of the host star’s light into the coronagraph dark hole may limit the ability of a potential Habitable Worlds Observatory (HWO) to detect and characterize an Earth-like planet around a Sun-like star. This paper summarizes a 5-year investigation of the impact of NAS on high-contrast coronagraphy. Science requirements were flowed down to a draft flux ratio noise ratio (FRN) error budget with allocations for NAS. The modeled sources of NAS include correlated (low and mid-spatial) surface structure, uncorrelated surface microroughness and coating structure, particulate contamination, micrometeoroid impacts and polarization leakage. The paper develops specifications for these sources of scattered light that meet their FRN error budget allocations, distinguishing between scatter that can and cannot be modulated. The development process utilizes an analytical expression that predicts scatter throughput into the dark hole based on BRDF/BSDF. Using this relationship, Photon Engineering performed a FRED® straylight analysis of the HWO EAC-1 (Exploratory Analytical Case #1) coronagraph to parametrically study predicted scattered light contrast in the dark hole. The model revealed that primary mirror microroughness or contamination is not a significant source of scattered light; the most critical surfaces are the smallest and those located closest to the coronagraph focal plane mask. The assessment found that uncorrelated microstructure, contamination, and micrometeoroid impacts require more investigation and potential significant sources of unmodulated scattered light, and highlights that slow thermal wavefront drift is an order of magnitude more impactful than fast mechanical jitter.

coronagraphy↗
DOE OSTI2020

Recent Developments at the U.S. Navy Wave Energy Test Site.

The U.S. Navy’s Wave Energy Test Site (WETS) in Hawaii has hosted two wave energy conversion (WEC) devices since its June 2015 commissioning – the Fred. Olsen BOLT Lifesaver and the Northwest Energy Innovations (NWEI) Azura – each for two deployments. Several additional devices will be tested in the coming years, beginning with the Ocean Energy device in summer 2019. The Hawaii Natural Energy Institute (HNEI) provides research and logistics support to WETS. We will provide an overview of three major activities that we have recently undertaken in this capacity. First, we will discuss results from a project in which modifications were made to the hull and float of the Azura, aimed at improving power performance for a second WETS deployment. Second, HNEI undertook a redeployment of Lifesaver beginning in October 2018, with the dual intent of achieving improvements in reliability and power performance, while also conducting an important demonstration of the use of wave power for non-grid applications. HNEI partnered with the University of Washington to integrate their Adaptable Monitoring Package (AMP) into the hull of BOLT Lifesaver. Included for this deployment was a subsea inductive charging capability from WiBotic, Inc.. These systems are powered entirely by electricity generated by the Lifesaver itself. Finally, HNEI has undertaken design improvements for the deeper berth moorings at WETS, with principal engineering guidance from DNV GL. This has included extensive numerical analysis of strength and fatigue aimed at establishing moorings that can persist for as long as possible. The resulting design will be discussed.

Wave energy conversion devices, Alternative market↗
DOE OSTI2024

Refinement and Modeling of a Blackbody-Based Calibration Method in the InfraBREAD Detector

The Broadband Reflector Experiment for Axion Detection (BREAD) is an ongoing collaboration searching for the conversion of yet undiscovered axion-like dark matter particles to photons in the presence of a magnetic field. InfraBREAD, an experiment of BREAD, uses a superconducting nanowire single photon detector (SNSPD), a high-efficiency and low-noise device, to specifically detect infrared-range photons produced by $\mathcal{O}$(eV) axion-like particles. The unique BREAD reflector setup allows for the focusing of converted signal photons to a 1mm $\times$ 1mm SNSPD. However, when the detector is cooled to cryogenic temperatures during operation, uneven thermal contraction of reflector components may lead to a small shift in the location of the focal spot. A novel calibration method using blackbody radiation is proposed to locate the true focal spot of the detector \textit{in situ}. Through ray tracing simulations done in FRED Optical Engineering Software, this method is demonstrated to locate the focus to within $\SI{50}{\micro\metre}$ in the $z$ dimension.

Rao, Shardul↗
DOE OSTI2024

Assessing Effects of Climate Change on Legacy Waste at the Enewetak Atoll

The Republic of the Marshall Islands (RMI) is in the central Pacific Ocean ~4,500 km west of Hawaii. The Enewetak Atoll, located in the northwest part of the RMI, was the site for 43 nuclear weapon tests between 1948 and 1958. Fallout and deposition from the tests contaminated the island surfaces, lagoon waters and sediment, and nearby ocean waters at the atoll. In the 1970s, a cleanup effort collected radioactive waste and placed it in the Cactus Crater on Runit Island (also called the Runit Dome). In December 2021, Congress directed the U.S. Department of Energy to study the impacts of climate change on the Runit Dome nuclear waste disposal site. Pacific Northwest National Laboratory (PNNL) assembled a multidisciplinary team of climate scientists, ocean modelers, environmental scientists, and health physicists to assess the likely effects of remaining radionuclides at the Enewetak Atoll. PNNL’s approach focused on effects of tropical cyclones that were postulated to mobilize and transport contaminated lagoon sediments and result in human and biota exposure. PNNL’s study estimated (1) the radionuclide source term, (2) the effects of climate change on severe storms, (3) mobilization and transport of radionuclides, and (4) radiation dose to humans and biota. Radionuclides in the lagoon and/or ocean waters of the Enewetak Atoll were characterized by the U.S. Atomic Energy Commission (AEC) in 1972, Woods Hole Oceanographic Institution in 2015, and Lawrence Livermore National Laboratory in 2018. The RMI Nationwide Radiological Study was conducted in the early 1990s for radionuclides remaining in island soils. The 1972 AEC survey remains the most comprehensive source of radionuclide data on lagoon sediments. Climate change modeling at a regional scale in the central Pacific Ocean is limited. PNNL climate scientists simulated severe historical storms postulated to occur both in a recent climate (2015) and in the future (2090) using the Advanced Research Weather Research and Forecasting (WRF-ARW) model, employing a pseudo-global-warming technique. A postulated complete, future failure of the Runit Dome was also considered. PNNL developed a high-resolution regional ocean hydrodynamics model covering the entire RMI extended economic zone using the Finite Volume Coastal Ocean Model (FVCOM). The FVCOM model was run using global reanalysis data for current climate and WRF-ARW simulation for the future climate. PNNL also developed a radionuclide fate and transport model using the FVCOM Integrated Compartment Model (FVCOM-ICM) to simulate the current and future mobilization and transport of radionuclides sorbed to lagoon sediments and the exchange of radionuclides between the water and sediment. FVCOM-ICM-predicted radionuclide concentrations were then used to estimate radiation dose to humans and biota at all islands of the Enewetak Atoll. Under current climate conditions, annual radiation exposures for the southern islands including Enewetak (Fred) and Medren (Elmer) were below the current U.S. standards. Radiation doses were somewhat elevated starting at Runit Island northward and westward to Enjebi Island (Janet). The islands in the northwest quadrant, particularly Bokoluo (Alice) and Bokombako (Belle), remain relatively contaminated. The islands in the southwestern quadrant have low contamination. The highest contribution to radiation doses comes from consumption of locally grown foods. Two radionuclides, 90Sr and 137Cs, contributed the greatest fraction for most terrestrial foods. In current climate conditions, the storms temporarily increased radionuclide concentrations in the lagoon waters, increasing the radiation dose slightly. In future conditions, doses are expected to be smaller, primarily because of the radioactive decay of the shorter-lived radioisotopes of 90Sr and 137Cs. This could make all islands in the far northwest of the atoll – except Bokombako (Belle) and perhaps Bokoluo (Alice) – suitable for residency. For the f

Prasad, Rajiv↗
DOE OSTI2024

Modeling and Proof-of-Concept of a Blackbody-Based Calibration Method in the InfraBREAD Detector

The Broadband Reflector Experiment for Axion Detection (BREAD) is an ongoing collaboration searching for the conversion of yet undiscovered axion-like dark matter particles to photons in the presence of a magnetic field. InfraBREAD, a pilot experiment realization of BREAD, uses a superconducting nanowire single photon detector (SNSPD), a high-efficiency and low-noise device, to specifically detect infrared-range photons produced by $\mathcal{O}$(eV) axion-like particles. The unique BREAD coaxial reflector setup allows for the focusing of converted signal photons to a 1mm $\times$ 1mm SNSPD. However, when the detector is cooled to cryogenic temperatures during operation, uneven thermal contraction of reflector components may lead to a small shift in the location of the focal spot. A novel calibration method using blackbody radiation is proposed to locate the true focal spot of the detector \textit{in situ}. Through ray tracing simulations done in FRED Optical Engineering Software, this method is demonstrated to locate the focus to within $\SI{50}{\micro\metre}$ in the axial dimension. Additionally, it is demonstrated that the blackbody photon source used in this calibration must be at a temperature of at least $\SI{15}{\kelvin}$ to $\SI{40}{\kelvin}$, depending on the sensitivity of the SNSPD.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗
DOE OSTI2026

Influence of the broadly neutralizing antibody VRC01 on HIV breakthrough virus populations in antibody-mediated prevention trials

In the antibody mediated prevention (AMP) trials, the broadly neutralizing antibody (bNAb) VRC01 demonstrated protective efficacy against susceptible HIV strains. To understand how VRC01 shaped breakthrough infections, deep sequencing was performed on 172 participants (>100,000 gag-Δpol and rev-env-Δnef sequences), at diagnosis and over time, in the placebo and treatment arms of the African (HVTN703/HPTN081; NCT02568215) and Americas/Europe (HVTN704/HPTN085; NCT02716675) cohorts. A high frequency of multilineage infections was detected (38%), including co-infection with both VRC01 sensitive and resistant viruses. This high frequency is largely accounted for by low-abundance lineages. Although VRC01 does not significantly affect the genetic transmission bottleneck compared to placebo, higher VRC01 doses trend towards greater VRC01 neutralization differences among co-infecting lineages. Two-thirds of multilineage infections showed evidence of recombination at the diagnostic timepoint. In the treatment group there is evidence of recombinant viruses preferentially inheriting resistance-associated mutations. This study provides critical insights into viral genetic and antigenic diversity that needs to be targeted to achieve protection, and highlights the role of recombination in facilitating escape.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2025

Bayesian estimation of HIV acquisition dates for prevention trials

Accurate timing estimates of when participants acquire HIV in HIV prevention trials are necessary for determining antibody levels at acquisition. The Antibody-Mediated Prevention (AMP) Studies showed that a passively administered broadly neutralizing antibody can prevent the acquisition of HIV from a neutralization-sensitive virus. We developed a pipeline for estimating the date of detectable HIV acquisition (DDA) in AMP Study participants using diagnostic and viral sequence data. Using a Bayesian strategy that combines three streams of data (REN [rev/vpu/env/Δnef] sequence, GP [gag/Δpol] sequence, and diagnostic) where their 95% credible intervals overlap based on pre-specified criteria and decision rules. We evaluated the performance of our AMP pipeline using PacBio viral sequence data from 41 participants across two prospective acute HIV acquisition cohort studies, FRESH and RV217, with twice-weekly sampling. These cohort studies enrolled young women in South Africa and men and women in Kenya and Thailand, respectively, with a high likelihood of HIV acquisition. In evaluating performance, “true DDA” was the center of bounds between last-negative and first-positive RNA diagnostic tests (median time 4 days, range 2–7 days); bias was the mean difference between estimated and true DDA. Using diagnostic data alone yielded timing estimates with a bias of 2.4 days and root mean square error (RMSE) of 7.9 days. These results were improved using sequence + diagnostic data (bias 1.5 days, RMSE 6.9 days), as well as by restricting sequence-based estimation to samples from ≤5 weeks post-DDA (bias 0.2 days, RMSE 7.8 days).

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2025

Syntrophic bacterial and host–microbe interactions in bacterial vaginosis

Bacterial vaginosis (BV) is a common, polymicrobial condition of the vaginal microbiota that is associated with symptoms such as malodor and excessive discharge, along with increased risk of various adverse sequelae. Host–bacteria and bacteria–bacteria interactions are thought to contribute to the condition, but many of these functions have yet to be elucidated. Using untargeted metaproteomics, we identified 1068 host and 1418 bacterial proteins in a set of cervicovaginal lavage samples collected from 20 participants with BV and 9 who were negative for the condition. We identified Dialister micraerophilus as a major producer of malodorous polyamines and identified a syntrophic interaction between this organism and Fannyhessea vaginae that leads to increased production of putrescine, a metabolite characteristic of BV. Although formate synthesis has not previously been noted in BV, we discovered diverse bacteria associated with the condition express pyruvate formate-lyase enzymes in vivo and confirm these organisms secrete formic acid in vitro. Sodium hypophosphite efficiently inhibited this function in multiple taxa. We also found that the fastidious organism Coriobacteriales bacterium DNF00809 can metabolize formic acid secreted by Gardnerella vaginalis, representing another syntrophic interaction. We noted an increased abundance of the host epithelial repair protein transglutaminase 3 in the metaproteomic data, which we confirmed by enzyme-linked immunosorbent assay. Other proteins identified in our samples implicate Finegoldia magna and Parvimonas micra in the production of malodorous trimethylamine. Some bacterial proteins identified represent novel targets for future therapeutics to disrupt BV communities and promote vaginal colonization by commensal lactobacilli.

bacterial vaginosis↗
DOE OSTI2026

Dynamics of phage-host interactions in Bacteroides fragilis resolved by single-cell transcriptomics

The interactions between lytic phages and their hosts are typically studied in bulk culture, which obscures cell-cell differences in infection susceptibility or expression of protective factors. Here, we use bacterial single-cell RNA sequencing to profile the transcriptomes of ~50,000 cells from cultures of a human pathobiont, Bacteroides fragilis, infected with a lytic bacteriophage. From a single sampling, we quantified the asynchronous progression of phage infection in individual bacterial cells and reconstructed the infection timeline, characterizing both host and phage transcriptomic changes as infection unfolded. Further, we discovered phenotypic subpopulations of bacteria that remained uninfected. Each cell’s vulnerability to phage infection was influenced by expression of multiple genetic loci, most prominently phase-variable capsular polysaccharide (CPS) biosynthesis pathways and an operon predicted to encode fimbrial genes. These findings uncovered genome-wide phase variation and stochasticity that enable bacterial survival and re-growth without acquiring additional mutations. Overall, we establish bacterial single-cell RNA sequencing as a powerful platform for investigating the dynamics of host-phage interactions and revealing the roles of phase variation and stochasticity in bacterial defenses.

Bacteria↗