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Results for “Genetic Engineering”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 91 records · Page 5
All sourcesNASA NTRSDOE OSTI
NASA NTRS1992

More Genetic Engineering With Cloned Hemoglobin Genes

Cells modified to enhance growth and production of proteins. Method for enhancing both growth of micro-organisms in vitro and production of various proteins or metalbolites in these micro-organisms provides for incorporation of selected chromosomal or extrachormosomal deoxyribonucleic acid (DNA) sequences into micro-organisms from other cells or from artificial sources. Incorporated DNA includes parts encoding desired product(s) or characteristic(s) of cells and parts that control expression of productor characteristic-encoding parts in response to variations in environment. Extended method enables increased research into growth of organisms in oxygen-poor environments. Industrial applications found in enhancement of processing steps requiring oxygen in fermentation, enzymatic degradation, treatment of wastes containing toxic chemicals, brewing, and some oxidative chemical reactions.

Bailey, James E.↗
NASA NTRS1926

The Avro "Avian" Airplane : 65 HP. Armstrong-Siddeley "Genet" engine

The Avro Avian, designed by Mr. Chadwick of A.V. Roe & Co., Ltd. has a very low structural weight (estimated at 750 lbs. empty) but with sufficient structural integrity to be eligible of an "Aerobatics" certificate from the British Air Ministry. It can be configured as a monoplane, or a biplane with seaplane floats. It is designed for economical production.

AIRPLANES-AVRO AVIAN (BRIT )↗
NASA NTRS1994

Crystals of Human Serum Albumin for Use in Genetic Engineering and Rational Drug Design

This invention pertains to crystals of serum albumin and processes for growing them. The purpose of the invention is to provide crystals of serum albumin which can be studied to determine binding sites for drugs. Form 2 crystals grow in the monoclinic space P2(sub 1), and possesses the following unit cell constraints: a = 58.9 +/- 7, b = 88.3 +/- 7, c = 60.7 +/- 7, Beta = 101.0 +/- 2 degrees. One advantage of the invention is that it will allow rational drug design

Carter, Daniel C.↗
NASA NTRS2002

Tissue engineering skeletal muscle for orthopaedic applications

With current technology, tissue-engineered skeletal muscle analogues (bioartificial muscles) generate too little active force to be clinically useful in orthopaedic applications. They have been engineered genetically with numerous transgenes (growth hormone, insulinlike growth factor-1, erythropoietin, vascular endothelial growth factor), and have been shown to deliver these therapeutic proteins either locally or systemically for months in vivo. Bone morphogenetic proteins belonging to the transforming growth factor-beta superfamily are osteoinductive molecules that drive the differentiation pathway of mesenchymal cells toward the chondroblastic or osteoblastic lineage, and stimulate bone formation in vivo. To determine whether skeletal muscle cells endogenously expressing bone morphogenetic proteins might serve as a vehicle for systemic bone morphogenetic protein delivery in vivo, proliferating skeletal myoblasts (C2C12) were transduced with a replication defective retrovirus containing the gene for recombinant human bone morphogenetic protein-6 (C2BMP-6). The C2BMP-6 cells constitutively expressed recombinant human bone morphogenetic protein-6 and synthesized bioactive recombinant human bone morphogenetic protein-6, based on increased alkaline phosphatase activity in coincubated mesenchymal cells. C2BMP-6 cells did not secrete soluble, bioactive recombinant human bone morphogenetic protein-6, but retained the bioactivity in the cell layer. Therefore, genetically-engineered skeletal muscle cells might serve as a platform for long-term delivery of osteoinductive bone morphogenetic proteins locally.

Review↗
DOE OSTI2019

Plants with engineered endogenous genes

Genetically engineered plants expressing altered Glucan Water Dikinase and having elevated levels of starch are provided. Methods of genetically engineering plants to express altered Glucan Water Dikinase, and genetic constructs are provided. Methods of breeding genetically engineered plants homozygous for a mutated gene encoding an altered Glucan Water Dikinase are described. Methods of agricultural processing and animal feed using the genetically engineered plants are also provided.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2014

Characterization of Wastewater Treatment Plant Microbial Communities and the Effects of Carbon Sources on Diversity in Laboratory Models

We are developing a laboratory-scale model to improve our understanding and capacity to assess the biological risks of genetically engineered bacteria and their genetic elements in the natural environment. Our hypothetical scenario concerns an industrial bioreactor failure resulting in the introduction of genetically engineered bacteria to a downstream municipal wastewater treatment plant (MWWTP). As the first step towards developing a model for this scenario, we sampled microbial communities from the aeration basin of a MWWTP at three seasonal time points. Having established a baseline for community composition, we investigated how the community changed when propagated in the laboratory, including cell culture media conditions that could provide selective pressure in future studies. Specifically, using PhyloChip 16S-rRNA-gene targeting microarrays, we compared the compositions of sampled communities to those of inocula propagated in the laboratory in simulated wastewater conditionally amended with various carbon sources (glucose, chloroacetate, D-threonine) or the ionic liquid 1-ethyl-3-methylimidazolium chloride ([C2mim]Cl). Proteobacteria , Bacteroidetes , and Actinobacteria were predominant in both aeration basin and laboratory-cultured communities. Laboratory-cultured communities were enriched in γ- Proteobacteria . Enterobacteriaceae , and Aeromonadaceae were enriched by glucose, Pseudomonadaceae by chloroacetate and D-threonine, and Burkholderiacea by high (50 mM) concentrations of chloroacetate. Microbial communities cultured with chloroacetate and D-threonine were more similar to sampled field communities than those cultured with glucose or [C2mim]Cl. Although observed relative richness in operational taxonomic units (OTUs) was lower for laboratory cultures than for field communities, both flask and reactor systems supported phylogenetically diverse communities. These results importantly provide a foundation for laboratory models of industrial bioreactor failure scenarios.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2023

Enhanced microbial production of protocatechuate from engineered sorghum using an integrated feedstock-to-product conversion technology

Building a stronger bioeconomy requires production capabilities that are largely generated through microbial genetic engineering. Plant feedstocks can additionally be genetically engineered to generate desirable feedstock traits and provide precursors for direct microbial conversion into desired products. The oleaginous yeast Rhodosporidium toruloides is a promising organism for this type of conversion as it can grow on a wide range of deconstructed biomass and consume a variety of carbon sources. Here, we leveraged R. toruloides native p-coumaric acid consumption pathway to accumulate protocatechuate (PCA) from 4-hydroxybenzoate (4HBA) released from a sorghum feedstock line genetically engineered to overproduce 4HBA. We did so by generating and evaluating an R. toruloides strain that accumulates PCA, RSΔ12623. We then show that at two scales a cholinium lysinate pretreatment with enzymatic saccharification successfully extracts 95% of the 4HBA from the engineered sorghum biomass while producing deconstructed lignin that can be more efficiently depolymerized in a subsequent thermochemical reaction. We also demonstrate that strain RSΔ12623 can convert more than 95% of 4HBA to PCA while consuming >95% of the glucose and >80% of the xylose present in sorghum hydrolysates. Finally, to evaluate the scalability of such fermentations, we conducted the conversion of 4HBA to PCA in a 2 L bioreactor under controlled conditions. Importantly, this work demonstrates the potential of purposefully producing aromatic precursors in planta that can be liberated during biomass deconstruction for direct microbial conversion to desirable bioproducts.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
DOE OSTI2023

Recent Advances in Picochlorum renovo Strain Development

Microalgae are amongst the most efficient phototrophs for the reduction of CO2 to industrially relevant products and product intermediates. We have recently identified a novel species in the genus Picochlorum (Picochlorum renovo) that demonstrates high areal productivity (>30 g/m2/day), high temperature and salinity tolerances, and rapid growth rate (-2 hour doubling time): attributes essential for economically viable industrial scale deployment for low value commodities (e.g. fuel and chemical precursors). Following screening of >300 algal isolates to identify Picochlorum renovo, we developed baseline genetic tools necessary for genetic engineering of the nucleus and chloroplast, and further built upon these tools to establish a protein secretion system. We have also deployed genetic engineering tools to establish the utilization of phosphite as a selectable marker in the chloroplast and nuclear genomes, while concurrently acting as a potential crop protection strategy. We have further implemented these tools to enable the utilization of formate as a novel carbon source for phototroph cultivation. Explicitly, formate toxicity was assayed, followed by introduction of a formate dehydrogenase to allow formate utilization. Cultures supplemented with formate grew to a higher density when cultivated under ambient CO2, highlighting the potential for this strategy to increase growth. Additionally, we have established a synthetic transcription factor and core promoter system which lays the foundation for tunable, high expression engineering in the nuclear genome, and potentially can be applied for universal algal genetic engineering. Finally, current work has focused on direct photosynthetic production of chemicals, and generation of mutant libraries. Combined, our work has established a robust genetic toolbox for Picochlorum renovo, which we have deployed for strain development purposes to introduce biotechnologically relevant traits.

algae↗
DOE OSTI2026

Learning from nature’s plant engineers: Hijacking metabolism and development beyond genetics

Plant metabolism underpins the food, fiber, and fuel that support our economy, driving strong interest in new strategies to rewire plant metabolism for emerging applications. While most synthetic biology efforts are reliant on genetic engineering, plants can be manipulated in many other ways that remain comparatively underexplored. Across nature, diverse organisms, including bacteria, fungi, and insects, have evolved sophisticated mechanisms to exploit plant metabolic richness, reshaping it for purposes that span from basic nutrition to the construction of complex, novel structures for shelters. These interspecies interactions and non-model systems represent unique manners in which plants can be reprogrammed or hijacked by other organisms, offering inspiration for novel approaches to engineering plant metabolism. By better understanding the basis of how organisms induce these remarkable transformations in plants, we can expand the conceptual boundaries of synthetic biology and reveal alternative routes to manipulating plants for the production of a diverse array of valuable compounds and materials. Deeper insight into these mechanisms will yield novel blueprints for rethinking the scope and breadth in which we can redesign plant metabolism across many applications.

Sarkiss, Anthony E. [University of California, Ber↗
DOE OSTI2021

Reconfiguring the metabolism of photosynthetic microbes for their development as biotechnological platforms [Slides]

LANL is at the forefront of genetic engineering of microalgae. We have developed genetic engineering toolboxes for many strains, including Picochlorum soloecismus, Nannochloropsis salina, and Chlorella sorokiniana. We have implemented such toolboxes for generating mutants with favorable phenotypes. We have the opportunity to integrate metabolic features from different species (cyanobacteria>microalgae>plants) into a synthetic biology discovery and developmental platform. We can leverage the cyanobacterial metabolic “simplicity” to engineer complex organisms, i.e. for the production of renewable polymers and unrivaled ‘omics and machine learning scientific collaboration.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2017

Sequestration of carbon dioxide with hydrogen to useful products

Provided herein are genetically engineered microbes that include at least a portion of a carbon fixation pathway, and in one embodiment, use molecular hydrogen to drive carbon dioxide fixation. In one embodiment, the genetically engineered microbe is modified to convert acetyl CoA, molecular hydrogen, and carbon dioxide to 3-hydroxypropionate, 4-hydroxybutyrate, acetyl CoA, or the combination thereof at levels greater than a control microbe. Other products may also be produced. Also provided herein are cell free compositions that convert acetyl CoA, molecular hydrogen, and carbon dioxide to 3-hydroxypropionate, 4-hydroxybutyrate, acetyl CoA, or the combination thereof. Also provided herein are methods of using the genetically engineered microbes and the cell free compositions.

08 HYDROGEN↗
DOE OSTI2019

Sequestration of carbon dioxide with hydrogen to useful products

Provided herein are genetically engineered microbes that include at least a portion of a carbon fixation pathway, and in one embodiment, use molecular hydrogen to drive carbon dioxide fixation. In one embodiment, the genetically engineered microbe is modified to convert acetyl CoA, molecular hydrogen, and carbon dioxide to 3-hydroxypropionate, 4-hydroxybutyrate, acetyl CoA, or the combination thereof at levels greater than a control microbe. Other products may also be produced. Also provided herein are cell free compositions that convert acetyl CoA, molecular hydrogen, and carbon dioxide to 3-hydroxypropionate, 4-hydroxybutyrate, acetyl CoA, or the combination thereof. Also provided herein are methods of using the genetically engineered microbes and the cell free compositions.

Adams, Michael W. W.↗
NASA NTRS2014

Application of Synthetic Biology to Bioregenerative Life Support for Human Spaceflight

The conversion of carbon dioxide into higher value products is a key challenge for the development of closed-loop life support systems for human space flight. Much of the past research on bioregenerative life support systems has focused on plant growth chambers as a solution for CO2 removal and O2 generation, but photosynthetic microorganisms may also have a role to play in these functions. Cyanobacteria have the advantages of relatively high CO2 fixation rates and fairly well-developed molecular biology tools, allowing for genetic engineering approaches to strain improvement. Manned missions to Mars or other targets beyond low Earth orbit will require advances in the nutritional systems for life support on these longer duration missions. A key challenge will likely be supplementing pre-packaged meals with specific nutrients that will be deficient due to problems in long-term storage or low abundance. Vitamin K is one such nutrient that may be important as a supplement. Production of vitamin K for nutrient supplementation during spaceflight will likely require genetic engineering of microorganisms to increase vitamin titers. A microbial bioreactor system that could efficiently convert CO2 to nutritional supplements would be a valuable component for a future advanced life support system. We are exploring biological systems to determine the feasibility of using bioreactors to convert CO2 to higher-value products. We are examining the performance of photosynthetic bacteria engineered to produce sugars, determining rates of production and reliability. We are also engineering microbes to produce higher titers of vitamin K and other potentially important nutrients. The results of this research will offer demonstrations of potential technologies that could be developed further in the future. This work will also provide valuable information for understanding basic science questions about the use of genetically engineered microbes in the microgravity environment.

Bioregenerative life support↗
DOE OSTI2021

Nuclear and chloroplast genome engineering of a productive non-model alga Desmodesmus armatus: Insights into unusual and selective acquisition mechanisms for foreign DNA

Despite the tremendous potential of algae to contribute to a future bioeconomy, there are practical and theoretical limitations to how well naturally sourced species and strains can perform in an outdoor setting. The application of biotechnology to modulate and engineer algae metabolism or to increase performance, resilience, or produce novel compounds, offers opportunities to overcome some of the major commercialization barriers. There are numerous approaches reported in the literature having variable success on genetic engineering of algae with non-model algae often presenting unique challenges to genetic engineering. We report here on successful nuclear and chloroplast genomic integration of selection marker resistance in the non-model alga Desmodesmus armatus. Nuclear transformation was accomplished using both electroporation and Agrobacterium-mediated approaches. However, in all surviving transformants, DNA integration was accompanied by excision and/or rearrangement of the gene of interest and fluorescence reporter coding sequences. Similarly, chloroplast transformation was successfully accomplished using a biolistic DNA delivery method. For these transformants, we also observed off-target mutations in the chloroplast genome, not previously observed in other, more routinely used, algae species. Finally, we present insights into potential mechanisms for these observed truncations, rearrangements, and mutations in D. armatus.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2019

Engineered Ureolytic Microorganisms Can Tailor the Morphology and Nanomechanical Properties of Microbial-Precipitated Calcium Carbonate

We demonstrate for the first time that the morphology and nanomechanical properties of calcium carbonate (CaCO 3 ) can be tailored by modulating the precipitation kinetics of ureolytic microorganisms through genetic engineering. Many engineering applications employ microorganisms to produce CaCO 3 . However, control over bacterial calcite morphology and material properties has not been demonstrated. We hypothesized that microorganisms genetically engineered for low urease activity would achieve larger calcite crystals with higher moduli. We compared precipitation kinetics, morphology, and nanomechanical properties for biogenic CaCO 3 produced by two Escherichia coli ( E. coli ) strains that were engineered to display either high or low urease activity and the native producer Sporosarcina pasteurii . While all three microorganisms produced calcite, lower urease activity was associated with both slower initial calcium depletion rate and increased average calcite crystal size. Both calcite crystal size and nanoindentation moduli were also significantly higher for the low-urease activity E. coli compared with the high-urease activity E. coli . The relative resistance to inelastic deformation, measured via the ratio of nanoindentation hardness to modulus, was similar across microorganisms. These findings may enable design of novel advanced engineering materials where modulus is tailored to the application while resistance to irreversible deformation is not compromised.

59 BASIC BIOLOGICAL SCIENCES↗