Search NASA⌕ Search

SEARCH · Search NASA

Results for “Laboratory evolution”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 91 records · Page 5
All sourcesNASA NTRSDOE OSTI
DOE OSTI2021

Cross-Feeding of a Toxic Metabolite in a Synthetic Lignocellulose-Degrading Microbial Community

The recalcitrance of complex organic polymers such as lignocellulose is one of the major obstacles to sustainable energy production from plant biomass, and the generation of toxic intermediates can negatively impact the efficiency of microbial lignocellulose degradation. Here, we describe the development of a model microbial consortium for studying lignocellulose degradation, with the specific goal of mitigating the production of the toxin formaldehyde during the breakdown of methoxylated aromatic compounds. Included are Pseudomonas putida, a lignin degrader; Cellulomonas fimi, a cellulose degrader; and sometimes Yarrowia lipolytica, an oleaginous yeast. Unique to our system is the inclusion of Methylorubrum extorquens, a methylotroph capable of using formaldehyde for growth. We developed a defined minimal “Model Lignocellulose” growth medium for reproducible coculture experiments. We demonstrated that the formaldehyde produced by P. putida growing on vanillic acid can exceed the minimum inhibitory concentration for C. fimi, and, furthermore, that the presence of M. extorquens lowers those concentrations. We also uncovered unexpected ecological dynamics, including resource competition, and interspecies differences in growth requirements and toxin sensitivities. Finally, we introduced the possibility for a mutualistic interaction between C. fimi and M. extorquens through metabolite exchange. This study lays the foundation to enable future work incorporating metabolomic analysis and modeling, genetic engineering, and laboratory evolution, on a model system that is appropriate both for fundamental eco-evolutionary studies and for the optimization of efficiency and yield in microbially-mediated biomass transformation.

Methylorubrum extorquens↗
NASA NTRS1996

EOS Laser Atmosphere Wind Sounder (LAWS) investigation

In this final report, the set of tasks that evolved from the Laser Atmosphere Wind Sounder (LAWS) Science Team are reviewed, the major accomplishments are summarized, and a complete set of resulting references provided. The tasks included preparation of a plan for the LAWS Algorithm Development and Evolution Laboratory (LADEL); participation in the preparation of a joint CNES/NASA proposal to build a space-based DWL; involvement in the Global Backscatter Experiments (GLOBE); evaluation of several DWL concepts including 'Quick-LAWS', SPNDL and several direct detection technologies; and an extensive series of system trade studies and Observing System Simulation Experiments (OSSE's). In this report, some of the key accomplishments are briefly summarized with reference to interim reports, special reports, conference/workshop presentations, and publications.

Source record↗
DOE OSTI2021

BETO 2021 Peer Review - Improving Formate Upgrading by Cupriavidus Necator 2.3.2.111

Formate can be generated by electrocatalytic reduction of CO2 and has been proposed as a soluble intermediate for the storage of carbon and energy. Biological systems capable of assimilating formate could enable conversion of formate generated from low-cost renewable energy and waste CO2 to myriad fuels and chemicals. To that end, the goal of this project is to develop the natural formatotroph Cupriavidus necator as a robust microbial chassis for efficient conversion of formate to value-added products. Using a combination of laboratory evolution and rational engineering, we have identified mutations and genetic deletions that improve its growth rate and biomass yield on formate by 1.2X. Using RNA-seq transcriptomics, we are elucidating the mechanisms that underly these improvements and anticipate this will contribute to our understanding of formate conversion. We have also engineered C. necator to convert formate to the potential polymer precursor 2-hydroxymuconate semialdehyde (2HMS) by introducing an exogenous pathway and deleting competing pathways and demonstrated production and robust growth using a pH-stat bioreactor. Final techno-economic analysis and life-cycle assessment on biological production of 2HMS from formate will enable the DOE and other stake holders to evaluate the economic and environmental impact of this project and related technologies.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗
NASA NTRS2010

Global Clusters as Laboratories for Stellar Evolution

Globular clusters have long been considered the closest approximation to a physicist's laboratory in astrophysics, and as such a near-ideal laboratory for (low-mass) stellar evolution, However, recent observations have cast a shadow on this long-standing paradigm, suggesting the presence of multiple populations with widely different abundance patterns, and - crucially - with widely different helium abundances as welL In this review we discuss which features of the Hertzsprung-Russell diagram may be used as helium abundance indicators, and present an overview of available constraints on the helium abundance in globular clusters,

Catelan, Marcio↗
DOE OSTI2023

Laboratory Experiments Examining the Effect of Thermal and Mechanical Processes on Hydraulic Transmissivity Evolution

Using laboratory slide-hold-slide experiments, at temperatures from 22 to 200 degrees C, to examine effects of fracture reactivation and quasi-static loading on the evolution of fluid transport properties of simulated fractures in Westerly granite. At all temperatures, the in-plane hydraulic transmissivity consistently decays during hold periods resulting in an overall reduction in transmissivity. During the first three to fifteen hours of an experiment, transmissivity decreases rapidly due to the generation of wear products, development of a sliding surface, and compaction of the resulting gouge. Once the sliding surface has developed, the long-term transmissivity decay rate at 22 and 100 degrees C is significantly lower than the transmissivity decay rate during the initial 3-15 hours of the experiment. However, at 200 degrees C, the decay of hydraulic transmissivity remains high throughout the experiment. The long-term decay of hydraulic transmissivity can be fitted with a power law model with more rapid reduction of hydraulic transmissivity at higher temperature. Periods of sliding on the fracture surface result in transient increases in the transmissivity, due to shear dilation, as is expected for Coulomb materials. These transients are superimposed on the long-term decay. When sliding ceases and a new hold period commences, there is a rapid reduction in transmissivity and return to the long-term rate of transmissivity decay. The rate of decay of the transmissivity transients is inversely correlated with temperature, in contrast to the long-term decay and the expected behavior for processes like subcritical crack growth and indentation creep. The higher decay rates that are observed during the initial 3-15 hours of the tests and following sliding, are associated with times that the porosity of the gouge is expected to be high. The difference in decay rates suggests that when the gouge is driven far from equilibrium by active shearing, densification may be dominated by a different mechanism from long-term compaction.

15 GEOTHERMAL ENERGY↗
NASA NTRS2000

NGC 1614: A Laboratory for Starburst Evolution

The modest extinction and reasonably face-on viewing geometry make the luminous infrared galaxy NGC 1614 an ideal laboratory for study of a powerful starburst. HST/NICMOS observations show: (1) deep CO stellar absorption, tracing a starburst nucleus about 45 pc in diameter; (2) surrounded by an approx. 600 pc diameter ring of supergiant H II regions revealed in Pa-alpha line emission; (3) lying within a molecular ring indicated by its extinction shadow in H - K; and (4) all at the center of a disturbed spiral galaxy. The luminosities of the giant H II regions in the ring axe extremely high, an order of magnitude brighter than 30 Doradus; very luminous H II regions, comparable with 30 Dor, are also found in the spiral arms of the galaxy. Luminous stellar clusters surround the nucleus and lie in the spiral arms, similar to clusters observed in other infrared luminous and ultraluminous galaxies. The star forming activity may have been initiated by a merger between a disk galaxy and a companion satellite, whose nucleus appears in projection about 300 pc to the NE of the nucleus of the primary galaxy. The relation of deep stellar CO bands to surrounding ionized gas ring to molecular gas indicates that the luminous starburst started in the nucleus and is propagating outward into the surrounding molecular ring. This hypothesis is supported by evolutionary starburst modeling that shows that the properties of NGC 1614 can be fitted with two short-lived bursts of star formation separated by 5 Myr (and by inference by a variety of models with a similar duration of star formation). The total dynamical mass of the starburst region of 1.3 x 10(exp 9) solar masses is mostly accounted for by the old pre-starburst stellar population. Although our starburst models use a modified Salpeter initial mass function (turning over near one solar mass), the tight mass budget suggests that the IMF may contain relatively more 10 - 30 solar masses stars and fewer low mass stars than the Salpeter function. The dynamical mass is nearly four times smaller than the mass of molecular gas estimated from the standard ratio of (C-12)O (1 - 0) to H2. A number of arguments place the mass of gas in the starburst region at approx. 25% of the dynamical mass, nominally about 1/15 and with an upper limit of 1/10 of the amount estimated from (C-12)O and the standard ratio.

Alonso-Herrero, A.↗
NASA NTRS1990

Regolith evolution in the laboratory - Scaling dissimilar comminution experiments

Repeated impacts into fragmental targets simulating unconsolidated debris on planetary surfaces have provided empirical insight into the evolution of planetary regoliths. The techniques of dimensional analysis have been employed to quantify and examine the relationships between the more important variables in the evolution of these experimental regoliths. Application of this method to the results of 10 experimental series shows that the quantity of comminuted target mass is directly proportional to (1) the number of impacts, (2) the diameter of the projectile, (3) the mean size of the crystals, (4) the mean grain size of the evolving regolith, (5) the total target mass, (6) the impactor density, and (7) the ratio of the impact velocity to the velocity of sound in the target rock. The comminuted mass is inversely proportional to the density of the target rock and the sorting of the regolith.

Cintala, Mark J.↗
NASA NTRS2019

PSR J2234+0611: A New Laboratory for Stellar Evolution

We report the timing results for PSR J2234+0611, a 3.6 ms pulsar in a 32 day, eccentric (e = 0.13) orbit with a helium white dwarf. The precise timing and eccentric nature of the orbit allow measurements of an unusual number of parameters: (a) a precise proper motion of 27.10(3) mas yr−1 and a parallax of 1.05(4) mas resulting in a pulsar distance of 0.95(4) kpc; enabling an estimate of the transverse velocity, 123(5) km s−1. Together with previously published spectroscopic measurements of the systemic radial velocity, this allows a 3D determination of the system’s velocity; (b) precise measurements of the rate of advance of periastron yields a total system mass of 1.6518 +0.0033-0.0035 Msun; (c) a Shapiro delay measurement, h3 = 82 ± 14 ns, despite the orbital inclination not being near 90°; combined with the measurement of the total mass yields a pulsar mass of 1.353 +0.014-0.017 Msun and a companion mass of 0.298 +0.015-0.0120 Msun; (d) we measure precisely the secular variation of the projected semimajor axis and detect a significant annual orbital parallax; together these allow a determination of the 3D orbital geometry of the system, including an unambiguous orbital inclination (i = 138.7 +2.5-2.2 deg) and a position angle for the line of nodes ( omega = 44 +5-4 deg). We discuss the component masses to investigate the hypotheses previously advanced to explain the origin of eccentric MSPs. The unprecedented determination of the 3D position, motion, and orbital orientation of the system, plus the precise pulsar and WD masses and the latter’s optical detection make this system a unique test of our understanding of white dwarfs and their atmospheres.

K Stovall↗
NASA NTRS2019

The Chemical Evolution of Cosmic Carbon: Laboratory Studies with the COSmIC Facility

Cosmic dust plays an essential role in our understanding of the chemical and physical evolution of the universe. Our understanding of cosmic dust relies on a combination of astronomical observations (either remote or on extraterrestrial dust samples), astrophysical modeling and laboratory studies of realistic analogs of cosmic dust grains. The COSmIC facility was developed at NASA Ames to study the evolution of cosmic carbon from circumstellar outflows to interstellar clouds to planetary atmospheres in the laboratory [1]. COSmIC stands for “Cosmic Simulation Chamber” and is dedicated to the study of neutral and ionized molecules and grain particles under the low temperature and density conditions that are required to simulate space environments. COSmIC integrates a variety of instruments that allow generating, processing, and monitoring simulated space conditions in the laboratory. It is composed of a Pulsed Discharge Nozzle (PDN) expansion that generates a plasma in a free supersonic jet expansion, coupled to high-sensitivity, complementary in situ diagnostic tools used for the detection and characterization of the species present in the expansion: Cavity Ring Down Spectroscopy (CRDS) and fluorescence spectroscopy systems for photonic detection and an orthogonal Reflectron Time-Of-Flight Mass Spectrometer (oReTOF-MS) for mass detection. Recent advances achieved in laboratory astrophysics using COSmIC’s laboratory data in synergy with observational data will be presented. These results include the formation of dust grains and aerosols from gas-phase molecular precursors in environments as varied as circumstellar outflows of late AGB stars [2] and planetary atmospheres [3] and the evolution of our understanding of the diffuse interstellar bands (DIBs) with applications to the ESO Diffuse Interstellar Bands Large Exploration Survey (EDIBLES [4, 5]). Plans for future laboratory developments and techniques to study the evolution of cosmic carbon molecules and grains (including NIR-MIR CRDS, laser induced fluorescence (LIF) and incandescence (LII)) will also be addressed as well as their astronomical applications.

Salama, F.↗
NASA NTRS1994

Open clusters as laboratories: The angular momentum evolution of young stars

This is the annual status report for the third year of our LTSA grant 'Open Clusters as Laboratories.' Because we have now had a few years to work on the project, we have started to produce and publish a large number of papers. We have been extremely successful in obtaining ROSAT observations of open clusters. With the demise of the PSPC on ROSAT, our main data source has come to an end and we will be able to concentrate on analyzing those data.

Stauffer, John R.↗
DOE OSTI2021

Evolution of an arched magnetized laboratory plasma in a sheared magnetic field

Arched magnetized structures are a common occurrence in space and laboratory plasmas. Results from a laboratory experiment on spatio-temporal evolution of an arched magnetized plasma ( $\beta \approx 10^{-3}$ , Lundquist number $\approx 10^{4}$ , plasma radius/ion gyroradius $\approx 20$ ) in a sheared magnetic configuration are presented. The experiment is designed to model conditions relevant to the formation and destabilization of similar structures in the solar atmosphere. The magnitude of a nearly horizontal overlying magnetic field was varied to study its effects on the writhe and twist of the arched plasma. In addition, the direction of the guiding magnetic field along the arch was varied to investigate its role in the formation of either forward- or reverse-S shaped plasma structures. The electrical current in the arched plasma was well below the current required to make it kink unstable. A significant increase in the writhe of the arched plasma was observed with larger magnitudes of overlying magnetic field. A forward-S shaped arched plasma was observed for a guiding magnetic field oriented nearly antiparallel to the initial arched plasma current, while the parallel orientation yielded the reverse-S shaped arched plasma.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗
DOE OSTI2019

Laboratory Foamed-Cement-Curing Evolution Using CT Scanning: Insights From Elevated-Pressure Generation

Computed-tomography (CT) scanning has become a mainstay among scientists (Wildenschild and Sheppard 2013) because it enables nondestructive observation of material processes and formation in real time. Foamed cement is a high-strength, low-density material containing nitrogen gas, and is used to stabilize wellbore casings both onshore and offshore. In-situ foamed cement is subject to pressure changes because the slurry is pumped downhole and cures at depth. To correlate the influence of pressure to the gas-void size and the curing evolution of a foamed cement, two laboratory foamed cements were generated and CT scanned as they cured. One cement was generated following the American Petroleum Institute (API) industry standard API RP 10B-4 (2015) at atmospheric conditions using a blender, and the other cement was created using a foamed-cement generator (FCG) (resembling that of de Rozières and Ferrière 1991) to produce a sample at an elevated pressure. FCG-generated cement qualities (in the range of 20, 25, 30, 35, and 40%) were scanned at their cured state for comparison. From the CT-image time series and the single FCG scans, the volumetric void properties were characterized and compared. The time-series blender voids were an order of magnitude larger than the FCG voids, and void growth was stagnant after a curing period of 100 minutes, whereas the FCG voids gradually increased in volume after 100 minutes. The Hsü-Nadai plots (Brandon 1995) reveal that the FCG and blender voids are weakly prolate, and all FCG voids, regardless of generation quality, relax to a greater final-magnitude strain than the blender voids. Furthermore, these findings confirm that both the void size and the curing process are influenced by the pressure at which a foamed cement is generated.

36 MATERIALS SCIENCE↗
DOE OSTI2019

Pore habit of methane hydrate and its evolution in sediment matrix – Laboratory visualization with phase-contrast micro-CT

Describing the pore habit of methane hydrate in sediment matrices is essential for understanding natural distribution of methane hydrate, methane trace (transport and solidification) in the hydrate stability zone, physical properties of hydrate-bearing sediments, and the associated influence on potential gas production. Pore habit visualization in natural media at pore scale even with laboratory synthesized cores has been challenging due to the similar densities of methane hydrate from pore liquid. In this work, we used phase-contrast assisted micro-CT with potassium iodine-doped brine to visualize four phases: sand particles, pore fluid, methane hydrate and methane gas. This study visualizes the pore habit of methane hydrate at various stages including during hydrate formation in excess-gas systems, its evolution after brine injection to replace pore fluid, and hydrate formation in excess-water systems. Hydrate tends to adopt round and smooth surfaces when in contact with water while exhibits relatively angular interfaces when in contact with methane gas. Hydrate formation in excess-gas systems results in a partial cementing and partial mineral-coating pore habit, while hydrate in excess-water systems develops mainly as pore-filling, and locally cementing or mineral-coating where big gas pockets exist at the initial state. Pore liquid replacement from methane gas to brine triggers a shift of hydrate pore habit towards pore-filling. Methane hydrate evolution over time produces bigger hydrate particles but with less contact area with sand particles. The effects of hydrate pore habit become less important as hydrate particle size exceeds the pore size. Additionally, hydrate formation could trap residual methane gas and brine as inclusions.

58 GEOSCIENCES↗
NASA NTRS1992

Evolution of catalytic RNA in the laboratory

We are interested in the biochemistry of existing RNA enzymes and in the development of RNA enzymes with novel catalytic function. The focal point of our research program has been the design and operation of a laboratory system for the controlled evolution of catalytic RNA. This system serves as working model of RNA-based life and can be used to explore the catalytic potential of RNA. Evolution requires the integration of three chemical processes: amplification, mutation, and selection. Amplification results in additional copies of the genetic material. Mutation operates at the level of genotype to introduce variability, this variability in turn being expressed as a range of phenotypes. Selection operates at the level of phenotype to reduce variability by excluding those individuals that do not conform to the prevailing fitness criteria. These three processes must be linked so that only the selected individuals are amplified, subject to mutational error, to produce a progeny distribution of mutant individuals. We devised techniques for the amplification, mutation, and selection of catalytic RNA, all of which can be performed rapidly in vitro within a single reaction vessel. We integrated these techniques in such a way that they can be performed iteratively and routinely. This allowed us to conduct evolution experiments in response to artificially-imposed selection constraints. Our objective was to develop novel RNA enzymes by altering the selection constraints in a controlled manner. In this way we were able to expand the catalytic repertoire of RNA. Our long-range objective is to develop an RNA enzyme with RNA replicase activity. If such an enzyme had the ability to produce additional copies of itself, then RNA evolution would operate autonomously and the origin of life will have been realized in the laboratory.

Joyce, Gerald F.↗
NASA NTRS1998

Open Clusters as Laboratories: The Angular Momentum Evolution of Young Stars

The core group concentrated on three primary research topics: (1) ROSAT observation of the coronal activity of low mass stars in young open clusters; (2) the determination of stellar ages and the determination of the timescale for dissipation of circumstellar disks around young stars; and (3) the determination of rotation velocities of low mass stars in young open cluster and the inferred angular momentum evolution of low mass stars. With accurate ages for the clusters, we can then derive an independent estimate of the timescale for debris disks to dissipate. As the second half of that project, we are using the Caltech/UC/NASA Keck telescopes to obtain spectra of brown dwarf candidates in a number of nearby, young open clusters, from which we can determine new and accurate cluster ages. The final primary program that we have addressed was the determination of rotational velocities for low mass stars in our target open clusters. Our group has obtained rotational velocities for a large number of stars in several open clusters during this LTSA program, and we have published the results in several papers. One particularly time-consuming aspect of our program was the development of a database of the photometry and rotational velocities for nearby open clusters, which we have made available to the community.

Stauffer, John R.↗