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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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77 records · Page 5
All sourcesNASA NTRSDOE OSTI
NASA NTRS2011

Deep Diversity: Novel Approach to Overcoming the PCR Bias Encountered During Environmental Analysis of Microbial Populations for Alpha-Diversity

Alpha-diversity studies are of crucial importance to environmental microbiologists. The polymerase chain reaction (PCR) method has been paramount for studies interrogating microbial environmental samples for taxon richness. Phylogenetic studies using this technique are based on the amplification and comparison of the 16S rRNA coding regions. PCR, due disproportionate distribution of microbial species in the environment, increasingly favors the amplification of the most predominant phylotypes with every subsequent reaction cycle. The genetic and chemical complexity of environmental samples are intrinsic factors that exacerbate an inherit bias in PCR-based quantitative and qualitative studies of microbial communities. We report that treatment of a genetically complex total genomic environmental DNA extract with Propidium Monoazide (PMA), a DNA intercalating molecule capable of forming a covalent cross-linkage to organic moieties upon light exposure, disproportionally inactivates predominant phylotypes and results in the exponential amplification of previously shadowed microbial ?-diversity quantified as a 19.5% increase in OUTs reported via phylogenetic screening using PhyloChip.

phylogenetic microarray technologies↗
NASA NTRS2015

Summer 2015 Internship Abstract

Green fluorescent protein (GFP) visually shows the expression of proteins by fluorescing when exposed to certain wavelengths of light. The GFP in this experiment was used to identify cells actively releasing viruses. The experiment focused on the effect of microgravity on the GFP expression of Akata B-cells infected with Epstein Barr Virus (EBV). Two flasks were prepared with 30 million cells each and two bioreactors were prepared with 50 million cells each. All four cultures were incubated for 16 days and fed every four days. Cellometer readings were taken on the feeding days to find cell size, viability, and GFP expression. In addition, the cells were treated with Propodium monoazide (PMA) and run through real time PCR to determine viral load on the feeding days. On the International Space Station air samples are taken to analyze the bacterial and fungal organisms in the air. The Sartorius Portable Airport is being investigated for potential use on the ISS to analyze for viral content in the air. Multiple samples were taken around Johnson Space Center building 37 and in Clear Lake Pediatric Clinic. The filter used was the gelatin membrane filter and the DNA was extracted directly from the filter. The DNA was then run through real time PCR for Varicella Zoster Virus (VZV) and EBV as well as GAPDH to test for the presence of DNA. The results so far have shown low DNA yield and no positive results for VZV or EBV. Further inquiry involves accurately replicating an atmosphere with high viral load from saliva as would be found on the ISS to run the air sampler in. Another line of research is stress hormones that may be correlated to the reactivation of latent viruses. The stress hormones from saliva samples are analyzed rather than blood samples. The quantity found in saliva shows the quantity of the hormones actually attached to cells and causing a reaction, whereas in the blood the quantity of hormones is the total amount released to cause a reaction. The particular hormones tested for were cortisol, alpha-amylase, and DHEA. The DHEA was very high in the two control samples tested. Regularly, samples came into the lab from local clinics to be tested for various viruses. Saliva, blood, body scrapes, and tears were received from the clinics and then run for VZV, EBV, and Human Simplex Virus 1 (HSV-1) with the results then reported back to the clinician. Blood, saliva, and urine from astronauts were also tested for viruses and logged. In addition, several cell cultures were brought up and grown, including adherent Human Lung Fibroblast (HFL) cells infected with VZV, and Akata B-cells infected with EBV.

Smith, Courtney↗
NASA NTRS2017

Surveys of ISS Returned Hardware for MMOD Impacts

Since February 2001, the Hypervelocity Impact Technology (HVIT) group at the Johnson Space Center in Houston has performed 26 post-flight inspections on space exposed hardware that have been returned from the International Space Station. Data on 1,024 observations of MMOD damage have been collected from these inspections. Survey documentation typically includes impact feature location and size measurements as well as microscopic photography (25-200x). Sampling of impacts sites for projectile residue was performed for the largest features. Results of Scanning Electron Microscopy (SEM) analysis to discern impactor source is included in the database. This paper will summarize the post-flight MMOD inspections, and focus on two inspections in particular: (1) Pressurized Mating Adapter-2 (PMA-2) cover returned in 2015 after 1.6 years exposure with 26 observed damages, and (2) Airlock shield panels returned in 2010 after 8.7 years exposure with 58 MMOD damages. Feature sizes from the observed data are compared to predictions using the Bumper risk assessment code.

Hyde, James↗
NASA NTRS2017

Surveys of Returned ISS Hardware for MMMOD Impacts

Since February 2001, the Hypervelocity Impact Technology (HVIT) group at the Johnson Space Center (JSC) in Houston has performed 35 post-flight inspections on space exposed hardware returned from the International Space Station (ISS). Data on 1,188 observations of micrometeoroid and orbital debris (MMOD) damage have been collected from these inspections. Survey documentation typically includes impact feature location and size measurements as well as microscopic photography (25-200x). Sampling of impacts sites for projectile residue was performed for the largest features. Results of energy dispersive X-ray spectroscopic analysis to discern impactor source are included in the database when available. This paper will focus on two inspections, the Pressurized Mating Adapter 2 (PMA-2) cover returned in 2015 after 1.6 years exposure with 26 observed impact features, and two Airlock shield panels returned in 2010 after 8.75 years exposure with 58 MMOD impacts. Feature sizes from the observed data are compared to predictions using the Bumper 3 risk assessment code.

Hyde, J. L.↗
NASA NTRS2013

Microbial Burden Approach : New Monitoring Approach for Measuring Microbial Burden

Advantages of new approach for differentiating live cells/ spores from dead cells/spores. Four examples of Salmonella outbreaks leading to costly destruction of dairy products. List of possible collaboration activities between JPL and other industries (for future discussion). Limitations of traditional microbial monitoring approaches. Introduction to new approach for rapid measurement of viable (live) bacterial cells/spores and its areas of application. Detailed example for determining live spores using new approach (similar procedure for determining live cells). JPL has developed a patented approach for measuring amount of live and dead cells/spores. This novel "molecular" method takes less than 5 to 7 hrs. compared to the seven days required using conventional techniques. Conventional "molecular" techniques can not discriminate live cells/spores among dead cells/spores. The JPL-developed novel method eliminates false positive results obtained from conventional "molecular" techniques that lead to unnecessary delay in the processing and to unnecessary destruction of food products.

microbial monitoring↗