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At least 91 records · Page 5

The evolution of analytical techniques for multiplex analysis of protein biomarkers

Introduction: The landscape of biomarker development has evolved with advanced analytical technologies, particularly affinity- and mass spectrometry-based techniques. These advancements have deepened our understanding of disease mechanisms, enabling the development of precise diagnostic tools and personalized medicine. Protein biomarkers, which play pivotal roles in biological processes, have become invaluable in diagnosing and monitoring diseases, aided by their presence in various biological samples and the availability of established detection methods. Areas covered: This review covers the role of protein biomarkers in clinical practice, the development and dimensionality of protein biomarkers, advancements in detection technologies, a comparison of these technologies, and future directions in biomarker discovery and disease mechanism elucidation. Expert opinion: Advances in biomarker technologies have the potential to transform diagnostics and personalized treatment but face challenges such as high costs and technical complexity. Enhancing reproducibility and integrating multi-omics approaches may offer better insights. In conclusion, the field should evolve toward high-throughput, automated methods, continuously adapting research, and clinical practices.

59 BASIC BIOLOGICAL SCIENCES↗

Reconstructing Hanford worker external doses from photons for epidemiology

The accurate reconstruction of external photon doses is essential for credible radiation epidemiology. This article presents the methodology used to derive dose estimates for 37 012 Hanford Site workers included in the Million Person Study. The approach employs historical dose records from the Hanford Radiation Exposure database and a previous epidemiology study. Bias correction factors specific to dosimeter type and period of use were applied and missing annual doses were estimated using a hierarchical nearby method to estimate deep dose equivalent for each worker. For early years with limited detection sensitivity, missed doses were quantified based on expected time-period-specific, low-dose statistical distributions. The revised dose estimates resulted in lower median and mean career doses than unadjusted data, while increasing the number of person-years with nonzero dose. Sensitivity analyses assessed the influence of bias in dosimetry measurements, missed doses and gap years on dose estimates. Differences in cumulative dose estimates between unadjusted and revised annual estimates are most prominent in the early operational years due to the highest bias during that time period.

dose reconstruction↗

Synthesizing land use and demographic change in Southeast Asia’s smaller urbanized areas from 2000–2015

The majority of the human population now reside in urban areas today. The United Nations estimates that nearly half of all urban dwellers currently live in cities smaller than 500 000 persons and the majority of future urban growth will take place in Asia and Africa, likely in these smaller urban areas, not mega cities. Thus, understanding the factors that influence urban demographic trajectories in small urban areas is critical to address sustainable and equitable policy initiatives related to food security, changing climate hazard exposure, and economic opportunities. Here we focus on Southeast Asia—a region historically characterized by lower urban population proportions, yet with a rapidly shifting dynamic demographic—to examine correlates of demographic change among smaller cities. We combine two open-source satellite-informed datasets: GHS urban center database (2015) and age-sex gridded data from WorldPop to calculate socio-demographic characteristics to model drivers of change in annualized urban population growth from 2000–2015 for 505 urbanized places. We find a general pattern of decreasing dependency ratios as city-size increases for most urban areas in Southeast Asia. Higher rates of growth and more variation is observed for smaller cities—those with fewer than 300 000 persons, the lowest population limit for UN data on urbanization. When examining covariates of urban population growth, we find significant statistical associations of population change in smaller urbanized areas with climatic, economic, and land cover/land use variables, but with country-specific variations. Characterizing a continuum of urban population development in the context of changing environmental, economic and climate conditions has been an important sustainable development and equity issue for decades, but newer analysis of city-level drivers allows for systematic inquiry thus moving beyond total population counts for policy-relevant insight.

Southeast Asia synthesis↗

CORSAIR: A Framework for Human Mobility Prediction through Visit Characterization and Spatial Behavior Modeling

The rapid advancement of location acquisition technologies has led to the daily collection of vast amounts of mobile trajectory data, facilitating in-depth research on human mobility and enabling more accurate mobility prediction models. However, existing methodologies often fall short in capturing the intricate dynamics of human navigation and spatial behavior. This paper addresses this gap by exploring the multifaceted relationships between individuals and their environments, considering the diverse influences of personal preferences and experiences. Some places hold sentimental value and are visited frequently, while others serve as transient points of passage. To model these differences, we introduce CORSAIR, a novel visit characterization framework that leverages visitation patterns and dwell times to delineate an individual’s relationship with specific places. CORSAIR classifies visits into seven distinct types: casual, occasional, routine, special, anchor, important, and resettling. Also, we show that explicitly recognizing these distinct visit types and incorporating nuanced visit intents into mobility prediction models leads to a substantial improvement in prediction accuracy. This distinction allows for more precise modeling of the individual’s transitions, enhancing the personalization and relevance of location-based services. Our findings suggest that a deeper understanding of the complexities of individual-environment interactions is crucial for developing effective predictive tools in mobility research.

Amichi, Licia [ORNL] (ORCID:0000000177631394)↗

Predictive analytics to direct clinical attention to complex patients with elevated suicide risk: enhancement of the Veterans Health Administration REACH VET model

Suicide is a major public health concern, particularly among Veterans. The U.S. Department of Veterans Affairs Veterans Health Administration (VHA) employs the Recovery Engagement and Coordination for Health–Veterans Enhanced Treatment (REACH VET) model to prioritise high-risk patients for targeted clinical attention. REACH VET 1.0 (RV 1.0) was developed on 2008–2011 data. To reflect changes in clinical practice and populations, VHA updated it to REACH VET 2.0 (RV 2.0). This study describes its development and validation. RV 2.0 used longitudinal data from 7,248,170 VHA patients (4,967 suicide deaths) in 2018–2019, with 650 time-varying demographic, clinical and area-level predictors derived from a 2-year lookback (2016–2019). An ensemble of Elastic-Net logistic regression models was trained on 2018 data and evaluated monthly at the population level in 2019, focusing on the top 0.1% intervention risk tier. Analyses assessed model discrimination, suicide detection, risk concentration, subgroup consistency (sex, age and race/ethnicity) and performance relative to RV 1.0 using the same percentile-based risk strata. RV 2.0 outperformed RV 1.0 across all risk strata, with better discrimination (C-statistic 0.76 vs 0.69) and consistent performance across demographic subgroups. Within the top 0.1% of predicted risk, RV 2.0 identified more deaths, higher suicide rates and greater mortality risk concentration both when averaged across the 12 monthly 2019 test sets (5.6 vs 3.6; 83.6 vs 53.7 per 100,000 person-years; 21.0 vs 14.1) and when annualised for 2019 (67 vs 43; 2.7% vs 1.7%; 1,003 vs 644 per 100,000 person-years; 26.7 vs 17.1). RV 2.0 improves suicide risk stratification among Veterans, demonstrating better performance and consistent prediction across subgroups and highlighting the need for regular model updates and evaluation.

Peluso, Alina [Oak Ridge National Laboratory (ORNL↗

From reactors to stars

This Eur. Phys. J. A volume 58 is dedicated to the life and work of Dr. Franz Käppeler from Karlsruhe Institute for Technology (KIT) who died on 20 November 2021, after a short illness. He was one of the leading experimentalists in the field of experimental nuclear astrophysics in Germany and worldwide for decades. Many of the authors of this volume knew Franz personally and many others were directly or indirectly inspired by his work and personality. All references in this introductory article refer to publications in this volume, not necessary to actual work by Dr. Käppeler.

99 GENERAL AND MISCELLANEOUS↗

In Silico Human Mobility Data Science: Leveraging Massive Simulated Mobility Data (Vision Paper)

Human mobility data science using trajectories or check-ins of individuals has many applications. Recently, we have seen a plethora of research efforts that tackle these applications. However, research progress in this field is limited by a lack of large and representative datasets. The largest and most commonly used dataset of individual human trajectories captures fewer than 200 individuals, while datasets of individual human check-ins capture fewer than 100 check-ins per city per day. Thus, it is not clear if findings from the human mobility data science community would generalize to large populations. Since obtaining massive, representative, and individual-level human mobility data is hard to come by due to privacy considerations, the vision of this work is to embrace the use of data generated by large-scale socially realistic microsimulations. Informed by both real data and leveraging social and behavioral theories, massive spatially explicit microsimulations may allow us to simulate entire megacities at the person level. The simulated worlds, which do not capture any identifiable personal information, allow us to perform “in silico” experiments using the simulated world as a sandbox in which we have perfect information and perfect control without jeopardizing the privacy of any actual individual. In silico experiments have become commonplace in other scientific domains such as chemistry and biology, permitting experiments that foster the understanding of concepts without any harm to individuals. This work describes challenges and opportunities for leveraging massive and realistic simulated alternate worlds for in silico human mobility data science.

97 MATHEMATICS AND COMPUTING↗

FiberFlex: Real-time FPGA-based Intelligent and Distributed Fiber Sensor System for Pedestrian Recognition

In recent years, security monitoring of public places and critical infrastructure has heavily relied on the widespread use of cameras, raising concerns about personal privacy violations. To balance the need for effective security monitoring with the protection of personal privacy, we explore the potential of optical fiber sensors for this application. This article proposes FiberFlex, an intelligent and distributed fiber sensor system. Ultizing Field Programmable Gate Arrays (FPGA) high-level synthesis (HLS) acceleration, FiberFlex offers real-time pedestrian detection by co-designing the entire pipeline of optical signal acquisition, processing, and recognition networks based on the principles of optical fiber sensing. As a promising alternative to traditional camera-based monitoring systems, FiberFlex achieves pedestrian detection by analyzing the vibration patterns caused by pedestrian footsteps, enabling security monitoring while preserving individual privacy. FiberFlex comprises three modules: First , fiber-optic sensing system: A fiber-optic distributed acoustic sensing (DAS) system is built and used to measure the ground vibration waves generated by people walking. Second , algorithms: We first collect the training data by measuring the ground vibration waves, label the data, and use the data to train the neural network models to perform pedestrian recognition. Third , hardware accelerators: We use HLS tools to design hardware modules on FPGA for data collection and pre-processing and integrate them with the downstream neural network accelerators to perform in-line real-time pedestrian detection. The final detection results are sent back from FPGA to the host CPU. We implement our system FiberFlex with the in-house built DAS system and AMD/Xilinx Kintex7 FPGA KC705 board and verify the whole system using the real-world collected data. We conduct recognition tests on five test subjects of varying ages, heights, and weights in a fixed sensing area. Each subject experienced 20 real-time recognition tests using their daily walking habits, and the subjects were given adequate rest between tests. After 100 tests on five test subjects, the overall real-time recognition accuracy exceeded \(88.0\%\) . The whole system uses 55 W of power, 33 W in the optical DAS system and 22 W in the FPGA. Relying on its end-to-end interdisciplinary design, FiberFlex seamlessly combines fiber-optic sensors with FPGA accelerators to enable low-power real-time security monitoring without compromising privacy, making it a valuable addition to the existing security monitoring network. According to FiberFlex, more valuable research can be conducted in the future, such as fall monitoring for the elderly, migration of identification networks between different application scenarios, and improvement of anti-interference performance in more complex environments. In future perception networks, where the “eyes” are not feasible, let’s use fiber optic touch instead.

Distributed↗

Characterization of two affinity matured Anti-Yersinia pestis F1 human antibodies with medical countermeasure potential

Yersinia pestis , the causative agent of plague and a biological threat agent, presents an urgent need for novel medical countermeasures due to documented cases of naturally acquired antibiotic resistance and potential person-to-person spread during a pneumonic infection. Immunotherapy has been proposed as a way to circumvent current and future antibiotic resistance. Here, we describe the development and characterization of two affinity matured human antibodies (αF1Ig AM2 and αF1Ig AM8) that promote survival of mice after exposure to aerosolized Y . pestis . We share details of the error prone PCR and yeast display technology-based affinity maturation process that we used. The resultant matured antibodies have nanomolar affinity for Y . pestis F1 antigen, are produced in high yield, and are resilient to 37°C stress for up to 6 months. Importantly, in vitro assays using a murine macrophage cell line demonstrated that αF1Ig AM2 and αF1Ig AM8 are opsonic. Even more importantly, in vivo studies using pneumonic plague mouse models showed that 100% of the mice receiving 500 μg of IgGs αF1Ig AM2 and αF1Ig AM8 survived lethal challenge with aerosolized Y . pestis CO92. Combined, these results provide evidence of the quality and robustness of αF1Ig AM2 and αF1Ig AM8 and support their development as potential medical countermeasures against plague.

59 BASIC BIOLOGICAL SCIENCES↗

WholeTraveler Anonymized Data Phase 1

Phase 1 of the WholeTraveler Study data collection consisted of an online-only survey. This survey captured data on three categories of observable variation in the population relevant to transportation decisions. First, the survey collected traditional demographic data such as age, gender, income, and education level. Second, it collected data across personality, psychological, and preference categories. This included: 1. The "Big Five" inventory personality traits: openness to new experience, conscientiousness, extroversion, agreeableness, and neuroticism; 2. Risk and time preferences; and 3. Environmental preferences. Third, the survey collected data on historical behavior patterns including: 1. Adoption of (as well as interest in) new technologies or innovations (e.g., smartphones, PEVs, solar panels, adaptive cruise control [ACC]); 2. Car ownership history and current car ownership status; 3. Recent mode use across different time scales (e.g., previous week, previous month, previous year); and 4. Timing of major life events such as starting a family as well as overall lifecycle trajectory patterns. Data from Phase 1 and Phase 2 are linked by a unique respondent identifier. Anonymized versions of the Phase 1 and Phase 2 data are both available on Livewire.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

2001 Atlanta Household Travel Survey

The 2001 Atlanta Household Travel Survey collected demographic, socioeconomic, and travel information on work and non-work travel behavior for a 48-hour travel period. The study was conducted on behalf of the Atlanta Regional Commission, and it is an essential element in the transportation planning and modeling efforts for the 13-county Atlanta region. The main objective of the study was to produce data that could be used to develop and calibrate travel demand models for use in travel forecasting, land use planning, and air quality planning to improve the transportation system. The second component in the survey was the deployment of an electronic travel diary with person-based GPS and an accelerometer to collect health and activity information. Travel data includes trip generation, trip distribution, and modal choice. The survey recruited a total of 12,184 households to participate in the study. Of these, 8,069 households (66%) completed travel. The 8,069 households, when weighted, represent 21,323 persons, 14,449 vehicles, and 126,127 places visited from April 2001 through April 2002. This dataset is part of the Metropolitan Travel Survey Archive, which includes travel surveys from numerous public agencies across the United States and is archived by the Transportation Secure Data Center to ensure their continued public availability.

1Hz data↗

2000 Knoxville Urban Area Household Travel Behavior Survey

This survey, conducted by NuStats, was a comprehensive study of travel behavior in Knox and Blount counties in the Knoxville urban area. The purpose of the survey was to collect weekday travel characteristics of household members (ages five and older) in the counties during a 24-hour timeframe. The data will be used by the Knoxville Urban Area Metropolitan Planning Organization and local agencies to update transportation and air quality models and to identify transportation needs in the region. The survey was conducted between November 2000 and February 2001. In total, 2,674 households were recruited to participate in the survey. Of these, 1,538 households reported data from a one-day travel log, totaling 12,631 trips by 3,522 persons. Of all trips originating in the study area, three-fourths were made by persons residing in Knox County.

1Hz data↗

2003 Yakima County Household Travel Survey

The Yakima County Household Travel Survey was sponsored by the Yakima County Department of Public Works in Yakima, Washington, and it was conducted over a six-month period from December 2002 through June 2003. The study is an essential element in the transportation planning and modeling efforts for Yakima County. The purpose of the survey was to gather data on the socioeconomic characteristics and travel behavior of county residents, including household characteristics, person characteristics, vehicle characteristics, and travel-activity patterns. A total of 1,503 households were recruited to participate in the study, 1,107 of which provided travel data for all household members age 16 and older. Unweighted, the 1,107 households represent 3,084 persons (of which 2,221 were age 16 and older), had 2,138 vehicles available to them, and recorded a total of 7,369 trips.

1Hz data↗

2023 Peer Review Final Report

The U.S. Department of Energy’s Office of Energy Efficiency and Renewable Energy requires each of its programs to conduct periodic peer reviews to enhance EERE program planning. May 16–18, 2023, the Advanced Materials and Manufacturing Technologies Office (AMMTO) held an in-person peer review of its program activities in which an independent panel of experts provided AMMTO with feedback on how well the new office’s programs align with its overarching goals and mission statement, identified possible course correction, and shared information. This review marks AMMTO’s first in-person peer review since the Advanced Manufacturing Office evolved into AMMTO and the Industrial Decarbonization and Efficiency Office. A detailed summary of observations, findings, impact, and recommendations is contained in the following report.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

Fueling Seniors: Meal Subsidy through Co-Generation

Formed in 1971, The Towers is an affordable HUD 202, 328-unit, living community with supportive services for extremely low, low and moderately low-income seniors. The average resident age is 79 and the average annual income is $\$$18,900. Approximately 70% of our residents receive assistance with their activities of daily living (e.g. hands-on bathing, feeding, dressing and toileting). We offer such services in a unique person-centered way through a wraparound support services model not always available in smaller low-income housing sites with assisted living (e.g. onsite service coordinators, engagement programs specific to the needs of our current and changing resident population and onsite 24-hour security who act as first response staff). The Towers serves the City of New Haven and surrounding areas by providing community-based senior care and support services that are affordable for individuals with limited resources. Our unique cost structure enables hundreds of seniors to avoid premature placement into nursing homes or other institutional care settings, even though they lack personal financial resources. This enables us to proactively keep our residents independent and healthy for as long as possible.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

Verifying LLM generative agents reflect human behavior in contested information environments to effectively simulate disinformation campaigns (Proteus)

Disinformation poses a significant and evolving threat to today’s online environment. Individuals encounter challenges in detecting disinformation, subsequently influencing their behavior and decision-making processes. Our research examines the potential use of large language model (LLM) generative agents (LGAs) to replicate human behavior to better understand how disinformation is spread in online environments. Using human subjects research, we first investigate how personality traits, individual differences, and demographic factors relate to decision-making in simulated online disinformation environments. Then, we examine whether LGAs can effectively replicate human responses in the same simulated online environments when assigned personality traits, demographic characteristics and behavioral attributes. Our findings indicate that LGAs can align with human decisions in these scenarios; however, alignment is contingent upon scenario context, persona settings and LLM selection. Results provide valuable insights for methodology refinement in future research and in utilizing LGAs to model complex national security challenges such as disinformation campaigns.

97 MATHEMATICS AND COMPUTING↗

New York State Household Travel Patterns: A Comprehensive Comparison Analysis

In 1969, the U.S. Department of Transportation began an initiative to collect detailed data on household and personal travel, with the most recent surveys being the 2017 and 2022 National Household Travel Surveys. The primary goal of these surveys is to collect trip-based data on the nature and characteristics of personal travel, allowing for an analysis of the relationships between travel patterns and the socio-economic and demographic characteristics of travelers and their households. In addition to the sample households allocated to New York State (NYS) in the national survey, they procured an additional sample of households in the 1995, 2001, 2009, and 2017 surveys. This report presents a comprehensive analysis of travel patterns, vehicle ownership trends, and travel behaviors across NYS regions and the rest of the nation.

99 GENERAL AND MISCELLANEOUS↗

2023 Annual Explosives Inventory Completion

The 2023 Lawrence Livermore National Laboratory (LLNL) annual explosives inventory was executed from May 18, 2023 to September 27, 2023 and was verified for accuracy effective September 28, 2023 following the LLNL Explosive Materials Inventory Plan. This year’s annual inventory includes changes incorporated based on the Department of Energy (DOE) Office of Inspector General (OIG) Audit Report DOE-OIG-20-50, The Department of Energy’s Storage and Disposition of Explosives Material at Selected Sites, dated July of 2020. Based on the associated recommendations, explosives at DOE and National Nuclear Safety Administration (NNSA) sites are considered "sensitive personal property" and applicable inventories must comply with 41 CFR 109, Personal Property Management. This regulation adds additional stipulations which require the annual inventory to include accountability of the total site inventory. In addition, the inventory must be performed by personnel other than the property owner, or alternatively must include independent verification. The development of the inventory plan was agreed to by the LLNL Explosives Safety Committee in conjunction with the LLNL Property and Business Division Leader. The LLNL Explosive Materials Inventory Plan was reviewed and approved by the DOE Explosives Safety Committee Chair on May 17, 2023 and by the NNSA Property Management Office on May 25, 2023.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗