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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 91 records · Page 5

A mechanism for strong shock electron heating in supernova remnants

It is shown that collisionless shock waves propagating away from a supernova may be directly responsible for the 10 keV X-ray emission seen in supernova remnants. A sequence of plasma instabilities (Buneman and ion acoustic) between the reflected and/or transmitted ions and the background electrons at the foot of the shock front can give rise to rapid anomalous heating of electrons. Hybrid simulations of a perpendicular collisionless shock are presented to demonstrate that this heating can arise within a self-consistently computed shock structure.

Cargill, P. J.↗

Correlations of peak heating in shock interference regions at hypersonic speeds.

Correlations are given of measured pressure and heat-transfer peaks for shock/boundary-layer interactions and shear layer attachment on configurations with both two- and three-dimensional interactions. The peak values were obtained from an investigation of shock interference heating on hemispheres, a 30-deg included angle wedge, and a 2.54-cm-diam cylindrical leading-edge fin model. The investigation covers data for Mach numbers of 6 and 20 over freestream Reynolds numbers ranging from (3.3 to 25.6) million per meter, and specific heat ratios of 1.4 and 1.67.

Keyes, J. W.↗

Sensitive, Rapid Detection of Bacterial Spores

A method of sensitive detection of bacterial spores within delays of no more than a few hours has been developed to provide an alternative to a prior three-day NASA standard culture-based assay. A capability for relatively rapid detection of bacterial spores would be beneficial for many endeavors, a few examples being agriculture, medicine, public health, defense against biowarfare, water supply, sanitation, hygiene, and the food-packaging and medical-equipment industries. The method involves the use of a commercial rapid microbial detection system (RMDS) that utilizes a combination of membrane filtration, adenosine triphosphate (ATP) bioluminescence chemistry, and analysis of luminescence images detected by a charge-coupled-device camera. This RMDS has been demonstrated to be highly sensitive in enumerating microbes (it can detect as little as one colony-forming unit per sample) and has been found to yield data in excellent correlation with those of culture-based methods. What makes the present method necessary is that the specific RMDS and the original protocols for its use are not designed for discriminating between bacterial spores and other microbes. In this method, a heat-shock procedure is added prior to an incubation procedure that is specified in the original RMDS protocols. In this heat-shock procedure (which was also described in a prior NASA Tech Briefs article on enumerating sporeforming bacteria), a sample is exposed to a temperature of 80 C for 15 minutes. Spores can survive the heat shock, but nonspore- forming bacteria and spore-forming bacteria that are not in spore form cannot survive. Therefore, any colonies that grow during incubation after the heat shock are deemed to have originated as spores.

Kern, Roger G.↗

Intracellular localization of a group II chaperonin indicates a membrane-related function

Chaperonins are protein complexes that are believed to function as part of a protein folding system in the cytoplasm of the cell. We observed, however, that the group II chaperonins known as rosettasomes in the hyperthermophilic archaeon Sulfolobus shibatae, are not cytoplasmic but membrane associated. This association was observed in cultures grown at 60 degrees C and 76 degrees C or heat-shocked at 85 degrees C by using immunofluorescence microscopy and in thick sections of rapidly frozen cells grown at 76 degrees C by using immunogold electron microscopy. We observed that increased abundance of rosettasomes after heat shock correlated with decreased membrane permeability at lethal temperature (92 degrees C). This change in permeability was not seen in cells heat-shocked in the presence of the amino acid analogue azetidine 2-carboxylic acid, indicating functional protein synthesis influences permeability. Azetidine experiments also indicated that observed heat-induced changes in lipid composition in S. shibatae could not account for changes in membrane permeability. Rosettasomes purified from cultures grown at 60 degrees C and 76 degrees C or heat-shocked at 85 degrees C bind to liposomes made from either the bipolar tetraether lipids of Sulfolobus or a variety of artificial lipid mixtures. The presence of rosettasomes did not significantly change the transition temperature of liposomes, as indicated by differential scanning calorimetry, or the proton permeability of liposomes, as indicated by pyranine fluorescence. We propose that these group II chaperonins function as a structural element in the natural membrane based on their intracellular location, the correlation between their functional abundance and membrane permeability, and their potential distribution on the membrane surface.

Cell Membrane/physiology/ultrastructure↗

Backshell Radiative Heating and Shock Layer Kinetic Measurement in NASA’s Electric Arc Shock Tube Facility

A methodology for creating incident shocks with similarity to the backshell radiative heating environment on planetary probes is presented. Two test series were carried out in the Electric Arc Shock Tube (EAST) at NASA Ames to quantify the backshell environment for planned and executed missions entering the atmospheres of Mars and Titan. The comparison of measured radiance in the shock tube to predicted post shock radiation levels is used to quantify the uncertainty in the model. This model uncertainty may then be related back to the flight condition to assess the error in the flight heating prediction. Tests for Mars entry show the shock tube measurements to be bracketed between two kinetic models by 2-7%. This suggests an 8% error in predicting the measured heating cannot be attributed to radiation. Comparisons to planned Titan entry suggest a substantial overprediction of the backshell radiation, which suggests opportunity for future refinement of radiative heating models.

radiation↗

Oxygen Deficiency in Spaceflight & its Impact on Plants’ Adaptive Changes

The goal of this study was to investigate the effects of hypoxic conditions in spaceflight. The distribution of genes involved with hypoxia in Arabidopsis thaliana and Brassica rapa were analyzed with the results from past spaceflight experiments to evaluate genes for future studies. Transcriptomes data of two different spaceflight studies of Arabidopsis thaliana from the NASA GeneLab database, GLDS-7 and GLDS-17, were compared. DNA microarrays were utilized for transcription profiling to conduct these studies. For GLDS-7, the response in spaceflight was studied with approaches that collected gene expression data. Leaves, hypocotyls, and root tissues were compared to the whole plant. For GLDS-17, seedlings and undifferentiated cultured cells were placed in the Biological Research in Canisters (BRIC), specifically BRIC-16. The genes related to hypoxia in Arabidopsis thaliana from these two studies were compared to genes in Brassica rapa with the TOAST database to evaluate similarities. When transcriptomes were analyzed for GLDS-7 and 17, genes that were considered significant had p-values ≤ 0.05 and log fold change values ≤ -1 or ≥1. Sixteen genes fulfilled the criteria. The genes related to hypoxia were alcohol dehydrogenase, elongation factor, ethylene-responsive factor, GUS, heat-shock proteins, NAP, RAP2.12, and RD20. The genes most impacted by spaceflight were heat-shock proteins. These genes were compared with Brassica rapa through Arabidopsis Ensemble Orthology from the TOAST Database. Similarities were seen in alcohol dehydrogenase, elongation factor, ethylene-responsive factor, heat-shock proteins, NAP, and RAP2.12. Overall, transcription profiling indicates that plants’ survival in spaceflight is dependent on adaptive changes with gene expression. This study also indicates that there are similarities in gene expression between Arabidopsis thaliana and Brassica rapa with comparable gene expression. Future studies could include analyzing additional species to understand which genes could be modified to ensure better yield of space crops amid hypoxic conditions.

hypoxia↗

Analytical and experimental studies of shock interference heating in hypersonic flows

An analytical and experimental study is presented of the aerodynamic heating resulting from six types of shock interference patterns encountered in high speed flow. Centerline measurements of pressure and heat transfer distributions on basic bodies were obtained in four wind tunnels for Mach numbers from 6 to 20, specific heat ratios from 1.27 to 1.67, and free stream Reynolds numbers from 3 million to 25.6 million per meter. Peak heating and peak pressures up to 17 and 7.5 times stagnation values, respectively, were measured. In general, results obtained from semiempirical methods developed for each of the six types of interference agreed with the experimental peaks.

Keyes, J. W.↗

Bolide impacts, acid rain, and biospheric traumas at the Cretaceous-Tertiary boundary

Two plausible projectiles are considered: an ice-rich long-period comet and a much smaller rock-metal asteroid. In the framework of a proposal addressed by Lewis et al. (1982), it is shown that, while the impact projectiles themselves do not shock-heat the atmosphere very extensively, the supersonic plume of water vapor and rock produced on impact does shock the atmosphere up to global scales and the shock is of sufficient intensity to produce abundant nitric oxide. For example, an ice-rich long-period comet with a mass of 1.25 x 10 to the 16th kg and a velocity of 65 km/s striking the earth would produce about 7 x 10 to the 40th molecules NO through shock-heating of the atmosphere by the high-velocity ejecta plume fragments. Specific attention is given to the fraction of the atmosphere shock-heated, the global circulation of the nitrogen oxides, the effects of the ejecta plume water on acid rain (AR) predictions, the effects of AR on continental soils, the relationship between AR production rates and the total amount of acid needed to acidify the surface oceans, and the longevity of the oceanic acidity event and the exhaled CO2 event and their implications for the environment in the first millenia or so after the impact.

Prinn, Ronald G.↗

Direct Measurement of the Ion Temperatures in Tycho's SNR

We present preliminary results of our XMM- Newton/RGS observation of the southeastern ejecta knots in Tycho's supernova remnant. The RGS successfully resolved numerous emission lines including O He-alpha, O Ly-alpha, and Fe L lines. Line broadening was measured to be ~3 eV for the O He-alpha and ~4.5 eV for Fe L lines. If we attribute the broadening to pure thermal Doppler effects, then we obtain kT_O and kT_Fe to be ~400 keV and 1.5 MeV, respectively. These temperatures can be explained by collisionless shock heating with a shock velocity of ~3600 km/s. This is inconsistent with (slightly smaller than) a forward shock speed of ~4300 km/s, suggesting that the knots are heated by a reverse shock. The relative abundance of intermediate-mass elements to oxygen in the knots is more consistent with that expected in a classical W7 model (a single-degenerate scenario) than a violent merger of two white dwarves (a double-degenerate scenario).

Brian Williams↗

Intracellular proteins produced by mammalian cells in response to environmental stress

The nature of the response of mammalian cells to environmental stress is examined by reviewing results of studies where cultured mouse L cells and baby hamster kidney cells were exposed to heat shock and the synthesis of heat-shock proteins and stress-response proteins (including HSP70, HSC70, HSP90, ubiquitin, and GRP70) in stressed and unstressed cells was evaluated using 2D-PAGE. The intracellular roles of the individual stress response proteins are discussed together with the regulation of the stress response system.

Goochee, Charles F.↗

Adiabatic invariants in stellar dynamics, 3: Application to globular cluster evolution

The previous two companion papers demonstrate that slowly varying perturbations may not result in adiabatic cutoffs and provide a formalism for computing the long-term effects of time-dependent perturbations on stellar systems. Here, the theory is implemented in a Fokker-Planck code and a suite of runs illustrating the effects of shock heating on globular cluster evolution are described. Shock heating alone results in considerable mass loss for clusters with R(sub g) less than or approximately 8 kpc: a concentration c = 1.5 cluster with R(sub g) kpc loses up to 95% of its initial mass in 15 Gyr. Only those with concentration c greater than or approximately 1.3 survive disk shocks inside of this radius. Other effects, such as mass loss by stellar evolution, will decrease this survival bound. Loss of the initial halo together with mass segregation leads to mass spectral indices, x, which may be considerably larger than their initial values.

Weinberg, Martin D.↗

Diffuse galactic annihilation radiation from supernova nucleosynthesis

The propagation of MeV positrons in the outer ejecta of type I supernovae was investigated. It was found that the positrons created at times of approx 100 days propagated along magnetic field lines in the outer ejecta without any appreciable pitch-angle scattering or excitation of hydromagnetic waves. The lack of significant pitch-angle scattering is well consistent with models of wave excitation and scattering by resonant interactions. This occurs because time periods to scatter the particles or to excite waves are significantly longer than escape times. Thus it is expected that, when positrons are not coupled to the ejecta by Coulomb collisions, they escape from the relatively cold, dense ejecta and reside predominantly in the tenuous, hotter, shock-heated interstellar gas. In the tenuous shock-heated gas the positron lifetime against annihilation is much greater than lifetimes in the dense ejectra. Thus the production of steady-state diffuse annihilation radiation by some fraction of these escaped positrons seems probable.

Higdon, J. C.↗

Computational studies of a fluid spike as a leading edge protection device for shock-shock interference heating

The effectiveness of a fluid spike as a device to protect leading edges of hypersonic atmospheric flight vehicles from high aerothermal loads produced by complex shock-shock interference is studied. The two-dimensional Navier-Stokes equations are solved using an unstructured cell-centered, fully implicit, flux-difference split algorithm. Adaptively generated unstructured meshes are employed. A type IV shock-shock interference for Mach 8 flow on a cylindrical leading edge with and without a small contraflow supersonic jet (fluid spike) placed at two different locations on the body is solved. A typical flow past a blunt body with a type IV shock-shock interference produces very high pressures and heat fluxes on the leading edge. Present results indicate that a fluid spike displaces the bow shock further in front of the body and modifies the shock-shock interference pattern. This leads to reduced peak pressures and heat fluxes on the body.

Prabhu, Ramadas K.↗

The Ca(2+) status of the endoplasmic reticulum is altered by induction of calreticulin expression in transgenic plants

To investigate the endoplasmic reticulum (ER) Ca(2+) stores in plant cells, we generated tobacco (Nicotiana tabacum; NT1) suspension cells and Arabidopsis plants with altered levels of calreticulin (CRT), an ER-localized Ca(2+)-binding protein. NT1 cells and Arabidopsis plants were transformed with a maize (Zea mays) CRT gene in both sense and antisense orientations under the control of an Arabidopsis heat shock promoter. ER-enriched membrane fractions from NT1 cells were used to examine how altered expression of CRT affects Ca(2+) uptake and release. We found that a 2.5-fold increase in CRT led to a 2-fold increase in ATP-dependent (45)Ca(2+) accumulation in the ER-enriched fraction compared with heat-shocked wild-type controls. Furthermore, after treatment with the Ca(2+) ionophore ionomycin, ER microsomes from NT1 cells overproducing CRT showed a 2-fold increase in the amount of (45)Ca(2+) released, and a 2- to 3-fold increase in the amount of (45)Ca(2+) retained compared with wild type. These data indicate that altering the production of CRT affects the ER Ca(2+) pool. In addition, CRT transgenic Arabidopsis plants were used to determine if altered CRT levels had any physiological effects. We found that the level of CRT in heat shock-induced CRT transgenic plants correlated positively with the retention of chlorophyll when the plants were transferred from Ca(2+)-containing medium to Ca(2+)-depleted medium. Together these data are consistent with the hypothesis that increasing CRT in the ER increases the ER Ca(2+) stores and thereby enhances the survival of plants grown in low Ca(2+) medium.

NASA Discipline Plant Biology↗

The effect of ablation injection on radiative and convective heating

A viscous shock-layer analysis is for calculating high energy equilibrium flow fields about blunt axisymmetric bodies is applied to the problem of massive ablation injection with radiation transport. A nongray radiation model is used that accounts for both line and continuum radiation. The solution method is direct and provides both stagnation and downstream solutions. Results for shock heated air show that phenolic-nylon injection is substantially more effective in reducing the wall radiant flux than air injection. Also, for large included body angles, the wall radiative flux and the coupled phenolic-nylon injection rate do not continue to decrease with increasing distance downstream.

Moss, J. N.↗

The effect of ablation injection on radiative and convective heating

A viscous shock-layer analysis for calculating high energy equilibrium flow fields about blunt axisymmetric bodies is applied to the problem of massive ablation injection with radiation transport. A nongray radiation model is used that accounts for both line and continuum radiation. The solution method is direct and provides both stagnation and downstream solutions. Results for shock heated air show that phenolic-nylon injection is substantially more effective in reducing the wall radiant flux than air injection. Also, for large included body angles, the wall radiative flux and the coupled phenolic-nylon injection rate do not continue to decrease with increasing distance downstream.

Moss, J. N.↗