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At least 91 records · Page 5

Studying Microbial Adaptation in the Laboratory: Sensor & Control Upgrades for an Experimental Evolution Biofluidics System

Experimental evolution (EE) involves iteratively exposing a microbial community to specific stressors to study its response to changes in environment over time. EE work is commonly done manually in the laboratory, but, when there are many environmental variables to measure and adjust, it is highly labor intensive, prone to human error, and challenging to scale. Single-purpose automated continuous culturing chambers exist, but implement only limited stressor types. A more general-purpose design is desirable. The BeING Lab at Ames Research Center created the prototype Automated Adaptive Directed Evolution Chamber (AADEC) to address these problems, beginning with Escherichia coli tolerance of short-wave ultraviolet (UV-C) radiation and of temperature. In newer versions, AADEC monitors microbial activity and can adjust the UV-C and temperature levels automatically. An optical density measurement is used to determine how many cells are present in the growth medium—over time, this corresponds to how many survive and reproduce. Oxidation-reduction potential provides information on consumed metabolic energy, and pH and electrical conductivity on metabolic products. Dissolved oxygen content is used to determine aerobic vs anaerobic growth. A Raspberry Pi computer processes all this data to set the UV-C stressor level. AADEC’s auxiliary systems include peristaltic pumps to change media and agitation to counteract cell settling. These actuators can also act as additional stressors. With the Raspberry Pi monitoring sensors and adjusting actuators in real time, AADEC takes measurements and controls the environment much more accurately than can be done with a manual EE implementation. The third and latest AADEC iteration is the first to simplify design and usage with circuits on PCBs and the ability to pre-program experimental protocols. Still planned is expansion to a multi-well design for the study of varying cell cultures in parallel, which will enable researchers to retain and re-inoculate cultures exhibiting the desired trait most strongly while flushing out others. AADEC’s special capabilities make it a valuable tool for studying life under multiple stressors, enabling scientists to replicate changes in climate on microbes for study in a lab setting.

Microbial Adaptation↗

A Comparison of Cloud Microphysical and Optical Properties during TOGA-COARE

The impact of cirrus clouds on climate is an issue of research interest currently. Whether cirrus clouds heat or cool the Earth-atmosphere system depends on the cloud shortwave albedo and infrared reflectance and absorptance. These in turn are determined by the size distribution, phase, and composition of particles in the clouds. The TOGA-COARE campaign presented an excellent opportunity to study cirrus clouds and their influence on climate. In this campaign, a microphysics instrument package was flown aboard the DC-8 aircraft at medium altitudes in cirrus clouds. This package included a 2D Greyscale Cloud Particle Probe, a Forward Scattering Spectrometer Aerosol Probe, and an ice crystal replicator. At the same time the ER-2 equipped with a radiation measurement system flew coordinated flight tracks above the DC-8 at very high altitude. The radiation measurement made were short and long wave fluxes, as well as narrowband fluxes, both upwelling and downwelling. In addition LIDAR data is available. The existence of these data sets allows for a the comparison of radiation measurement with microphysical measurements. For example, the optical depth and effective radius retrieved from the ER-2 radiation measurements can be compared to the microphysical data. Conversely, the optical properties and fluxes produced by the clouds can be calculated from the microphysical measurements and compared to those measured aboard the ER-2. The assumptions required to make these comparisons are discussed. Typical microphysical results show a prevalence of micron-sized particles, in addition to the cloud particles that exceed 100 mm. The large number of small particles or "haze" cause the effective cloud radii to shift to smaller sizes, leading to changes in optical parameters.

Strawa, A. W.↗

Seasonal cloud-radiative forcing over land and ocean derived from ERBE satellites

Earth Radiation Budget Experiment (ERBE) data are analyzed to determine seasonal variations of radiative parameters. Particular attention is given to annual variations of the top-of-the atmosphere zonally averaged outgoing long-wave (LW) and absorbed short-wave (SW) flux, as well as the short- and long-wave components of cloud-radiative forcing. The ERBE results reveal significant seasonal variations in both outgoing LW and absorbed SW flux, and a pronounced difference between oceanic and continental surfaces.

Harrison, Edwin F.↗

Analysis of Long-Term Cloud Cover, Radiative Fluxes, and Sea Surface Temperature in the Eastern Tropical Pacific

Grant activities accomplished during this reporting period are summarized. The contributions of the principle investigator are reported under four categories: (1) AHVRR (Advanced Very High Resolution Radiometer) data; (2) GOES (Geostationary Operational Environ Satellite) data; (3) system software design; and (4) ATSR (Along Track Scanning Radiometer) data. The contributions of the associate investigator are reported for:(1) longwave irradiance at the surface; (2) methods to derive surface short-wave irradiance; and (3) estimating PAR (photo-synthetically active radiation) surface. Several papers have resulted. Abstracts for each paper are provided.

Simpson, J. J.↗

The Surface Energy Budget and Precipitation Efficiency for Convective Systems During TOGA, COARE, GATE, SCSMEX and ARM: Cloud-Resolving Model Simulations

A two-dimensional version of the Goddard Cumulus Ensemble (GCE) Model is used to simulate convective systems that developed in various geographic locations. Observed large-scale advective tendencies for potential temperature, water vapor mixing ratio, and horizontal momentum derived from field campaigns are used as the main forcing. By examining the surface energy budgets, the model results show that the two largest terms are net condensation (heating/drying) and imposed large-scale forcing (cooling/moistening) for tropical oceanic cases. These two terms arc opposite in sign, however. The contributions by net radiation and latent heat flux to the net condensation vary in these tropical cases, however. For cloud systems that developed over the South China Sea and eastern Atlantic, net radiation (cooling) accounts for about 20% or more of the net condensation. However, short-wave heating and long-wave cooling are in balance with each other for cloud systems over the West Pacific region such that the net radiation is very small. This is due to the thick anvil clouds simulated in the cloud systems over the Pacific region. Large-scale cooling exceeds large-scale moistening in the Pacific and Atlantic cases. For cloud systems over the South China Sea, however, there is more large-scale moistening than cooling even though the cloud systems developed in a very moist environment. though For three cloud systems that developed over a mid-latitude continent, the net radiation and sensible and latent heat fluxes play a much more important role. This means the accurate measurement of surface fluxes and radiation is crucial for simulating these mid-latitude cases.

Tao, W.-K.↗

Sensing Clouds via Spacecraft Radio Occultation Observations

The presence of clouds dramatically alters the opacity and radiative transfer within Earth's atmosphere at both short and long wavelengths. Knowledge of cloud top and base is needed to estimate the Outgoing Long wave Radiation (OLR) to space and the net radiation at the surface from a given atmospheric profile. Satellite observations are required to provide the global perspective needed for climate. Cloud top pressure can be determined to some accuracy from spaceborne radiance measurements when cloud opacities are sufficiently large and cloud top temperatures are sufficiently low to readily them from the surface. Cloud base is particularly difficult to determine from space. The relatively small sensitivity of long wavelengths must be used to penetrate the clouds while at the same time providing sufficient sensitivity to detect the cloud base. We are studying the indirect sensitivity of radio occultation observations to clouds through their impact on the refractivity structure. The tradewind inversion is an excellent example where a very sharp refractivity structure coincides with the top of the tradewind cumulus and stratus clouds. In general, any cloud with sufficient IR opacity will have large transmissivity gradient at cloud top (base) which will result in large cooling (heating) which will create a thermal inversion at cloud top (base). Both liquid and ice clouds can reach this critical opacity. The thermal inversion and sharp change in specific humidity will cause a sharp change in the refractivity gradient which can be identified in the radio occultation results, particularly the signal amplitude. The occultations yield very accurate information on the height of such features. The heating at cloud base drives convection causing the air to follow a moist adiabat within the Cloud which further helps constrain the interpretation of the observations. The upward expansion of such opaque clouds due to convection may be an important mechanism supplying moisture into the upper troposphere. We will present simulations using high resolution radiosondes from field campaigns representing the expected signatures of such features. We will also present initial results of comparisons between GPS observations and GOES-derived cloud tops to assess the utility of this concept.

Kursinski, E. R.↗

Observation of local radio emission associated with type III radio bursts and Langmuir waves

The first clear detection of fundamental and harmonic radiation from the type III radio source region is presented. This radiation is characterized by its lack of frequency drift, its short rise and decay times, its relative weakness compared to the remotely observed radiation and its temporal coincidence with observed Langmuir waves. The observations were made with the radio and plasma frequency (URAP) receivers on the Ulysses spacecraft between about 1 and 2 AU from the Sun.

Reiner, M. J.↗

Test particle modeling of wave-induced energetic electron precipitation

A test particle computer model of the precipitation of radiation belt electrons is extended to compute the dynamic energy spectrum of transient electron fluxes induced by short-duration VLF wave packets traveling along the geomagnetic field lines. The model is adapted to estimate the count rate and associated spectrum of precipitated electrons that would be observed by satellite-based particle detectors with given geometric factor and orientation with respect to the magnetic field. A constant-frequency wave pulse and a lightning-induced whistler wave packet are used as examples of the stimulating wave signals. The effects of asymmetry of particle mirror heights in the two hemispheres and the atmospheric backscatter of loss cone particles on the computed precipitated fluxes are discussed.

Chang, H. C.↗

MESSENGER: Exploring Mercury's Magnetosphere

The MESSENGER mission to Mercury offers our first opportunity to explore this planet s miniature magnetosphere since the brief flybys of Mariner 10. Mercury s magnetosphere is unique in many respects. The magnetosphere of Mercury is among the smallest in the solar system; its magnetic field typically stands off the solar wind only - 1000 to 2000 km above the surface. For this reason there are no closed drift paths for energetic particles and, hence, no radiation belts. The characteristic time scales for wave propagation and convective transport are short and kinetic and fluid modes may be coupled. Magnetic reconnection at the dayside magnetopause may erode the subsolar magnetosphere allowing solar wind ions to impact directly the regolith. Inductive currents in Mercury s interior may act to modify the solar wind interaction by resisting changes due to solar wind pressure variations. Indeed, observations of these induction effects may be an important source of information on the state of Mercury s interior. In addition, Mercury s magnetosphere is the only one with its defining magnetic flux tubes rooted in a planetary regolith as opposed to an atmosphere with a conductive ionospheric layer. This lack of an ionosphere is probably the underlying reason for the brevity of the very intense, but short-lived, - 1-2 min, substorm-like energetic particle events observed by Mariner 10 during its first traversal of Mercury s magnetic tail. Because of Mercury s proximity to the sun, 0.3 - 0.5 AU, this magnetosphere experiences the most extreme driving forces in the solar system. All of these factors are expected to produce complicated interactions involving the exchange and re-cycling of neutrals and ions between the solar wind, magnetosphere, and regolith. The electrodynamics of Mercury s magnetosphere are expected to be equally complex, with strong forcing by the solar wind, magnetic reconnection at the magnetopause and in the tail, and the pick-up of planetary ions all driving field-aligned electric currents. However, these field-aligned currents do not close in an ionosphere, but in some other manner. In addition to the insights- into magnetospheric physics offered by study of the solar wind - Mercury system, quantitative specification of the "external" magnetic field generated by magnetospheric currents is necessary for accurate determination of the strength and multi-polar decomposition of Mercury s intrinsic magnetic field. MESSENGER S highly capable instrumentation and broad orbital coverage will greatly advance our understanding of both the origin of Mercury s magnetic field and the acceleration of charged particles in small magnetospheres. In. this article, we review what is known about Mercury s magnetosphere and describe the MESSENGER science team s strategy for obtaining answers to the outstanding science questions surrounding the interaction of the solar wind with Mercury and its small, but dynamic, magnetosphere.

Slavin, James A.↗

MESSENGER: Exploring Mercury's Magnetosphere

The MESSENGER mission to Mercury offers our first opportunity to explore this planet's miniature magnetosphere since Mariner 10's brief fly-bys in 1974-5. Mercury's magnetosphere is unique in many respects. The magnetosphere of Mercury is the smallest in the solar system with its magnetic field typically standing off the solar wind only - 1000 to 2000 km above the surface. For this reason there are no closed dri-fi paths for energetic particles and, hence, no radiation belts; the characteristic time scales for wave propagation and convective transport are short possibly coupling kinetic and fluid modes; magnetic reconnection at the dayside magnetopause may erode the subsolar magnetosphere allowing solar wind ions to directly impact the dayside regolith; inductive currents in Mercury's interior should act to modify the solar In addition, Mercury's magnetosphere is the only one with its defining magnetic flux tubes rooted in a planetary regolith as opposed to an atmosphere with a conductive ionosphere. This lack of an ionosphere is thought to be the underlying reason for the brevity of the very intense, but short lived, approx. 1-2 min, substorm-like energetic particle events observed by Mariner 10 in Mercury's magnetic tail. In this seminar, we review what we think we know about Mercury's magnetosphere and describe the MESSENGER science team's strategy for obtaining answers to the outstanding science questions surrounding the interaction of the solar wind with Mercury and its small, but dynamic magnetosphere.

Slavin, James A.↗

Albedo of the ocean-atmosphere system - Influence of sea foam

The influence of the ocean's optical properties and wind induced sea surface foam (white caps) on the short-wave albedo of the ocean-atmosphere system is studied by solving the radiative transfer equation using a Monte Carlo method. It is found that for a foam free ocean, the planetary albedo of a very clear ocean is at most 10 percent greater than that for a totally absorbing ocean. However, the introduction of a relatively small amount of sea foam on the surface can produce a considerable increase in the albedo, especially if the foam is highly reflecting. For example, it is shown that for foam which is totally reflecting (the foam albedo is 1), an increase in the wind speed from 6 to 14 m/sec would double the planetary albedo for small solar zenith angles.

Gordon, H. R.↗

Infrared Spectral Responses of the Ocean Color Instrument (OCI) Pre-assembly and Integration

The Ocean Color Instrument (OCI) to go on the Plankton, Aerosol, Cloud, ocean Ecology (PACE) Earth-observing satellite has a Short-wave infrared (SWIR) Detection Assembly (SDA). This SDA is used to measure upwelling radiation in seven discrete bands from 940 to 2260 nm. There are redundant measurements of each band for a total of 32 physical channels, which includes optical components through to detection. The relative spectral response (RSR) is measured for each channel, which is needed when accounting for the spectral distribution of sensed radiance. From the RSR, single-value performance metrics are computed including the center wavelength, the full width at half of the maximum (FWHM), and the full width at 1% of the maximum (FW1P). Besides in-band responses, the out-of-band rejection ratio (OOBRR) is also calculated for each of the channels, which is a measure of the sensitivity outside the band of interest. We find that all 32 SDA detection channels meet the spectral response requirements at the qualification temperatures at which tests were conducted.

PACE↗

Spectral Responses of the PACE OCI Short-Wave Infrared Detection Assembly

The Ocean Color Instrument (OCI) to go on the Plankton, Aerosol, Cloud, ocean Ecology (PACE) Earth-observing satellite has a Short-wave infrared (SWIR) Detection Assembly (SDA). This SDA is used to measure upwelling radiation in seven discrete bands from 940 to 2260 nm. There are redundant measurements of each band for a total of 32 physical channels, which includes optical components through to detection. The relative spectral response (RSR) is measured for each channel, which is needed when accounting for the spectral distribution of sensed radiance. From the RSR, single-value performance metrics are computed including the center wavelength, the full width at half of the maximum (FWHM), and the full width at 1% of the maximum (FW1P). Besides in-band responses, the out-of-band rejection ratio (OOBRR) is also calculated for each of the channels, which is a measure of the sensitivity outside the band of interest. We find that all 32 SDA detection channels meet the spectral response requirements at the qualification temperatures at which tests were conducted.

PACE↗

Radiation from long pulse train electron beams in space plasmas

A previous study of electromagnetic radiation from a finite train of electron pulses is extended to an infinite train of such pulses. The electrons are assumed to follow an idealized helical path through a space plasma in such a manner as to retain their respective position within the beam. This leads to radiation by coherent spontaneous emission. The waves of interest in this region are the whistler slow (compressional) and fast (torsional) Alfven waves. Although a general theory is developed, analysis is then restricted to two approximations, the short and long electron beam. Formulas for the radiation per unit solid angle from the short beam are presented as a function of both propagation and ray angles, electron beam pulse width and separation and beam current, voltage, and pitch angle. Similar formulas for the total power radiated from the long beam are derived as a function of frequency, propagation angle, and ray angle. Predictions of the power radiated are presented for representative examples as determined by the long beam theory.

Harker, K. J.↗

Gravity wave propagation in a diffusively separated atmosphere with height-dependent collision frequencies

Numerical calculations of gravity wave propagation through a two-component thermosphere with vertically-varying inter-species collision frequencies are performed using a direct integration method. Reflection of upgoing gravity wave energy into downgoing gravity waves appears to be small though nonnegligible at typical thermospheric periods and wavelengths. Coupling into diffusion waves is completely insignificant in most cases and may be determined in part by the relative abundance of the minor species. Temperature perturbation differences between species can be greater than 10% above 300 km altitude even though coupling into diffusion waves is small. Diffusive dissipation of gravity wave energy can be significant in the lower thermosphere and may be comparable to dissipation by viscosity and heat conduction below about 225 km altitude. Heating by diffusive dissipation may also be comparable to direct heating by solar radiation in the lower thermosphere. As a result of dissipative filtering of long-period and short-wavelength waves and the sensitivity of He-N2 density perturbation phase differences to diffusion, the AE-C satellite wave observations can only be fit by internal gravity waves with periods no greater than about 25 min and horizontal wavelengths no less than about 200 km.

Del Genio, A. D.↗

An economical net solar flux radiometer.

The theory and design of an economical solar flux radiometer readily adaptable to the conventional radiosonde system are presented in response to the need cited by the Atmospheric Radiation Working Group (1971) for the development of an inexpensive light-weight short-wave radiometer sonde. Prototype instrument tests and current modifications are discussed. It is shown that the basic instrument can be easily modified to measure separately the upward and downward directed flux components, in addition to the net flux.

Darkow, G. L.↗