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Immune responses in humans after 60 days of confinement

A confinement experiment in a normobaric diving chamber was undertaken to better understand the effect of confinement and isolation on human psychology and physiology. Pre- and postconfinement blood samples were obtained from four test subjects and control donors to analyze immune responses. No modification in the levels of CD2+, CD3+, CD4+, CD8+, CD19+, and CD56+ cells was observed after confinement. Mitogen-induced T-lymphocyte proliferation and interleukin-2 receptor expression were not altered significantly. Whole blood interferon-alpha and gamma-induction and plasma cortisol levels were also unchanged, as was natural killer cell activity. These data suggest that in humans, no specific components of the immune response are affected by a 2-month isolation and confinement of a small group.

NASA Discipline Number 18-10↗

Crew productivity issues in long-duration space flight

Considerable evidence suggests the importance of teamwork, coordination, and conflict resolution to the performance and survival of isolated, confined groups in high-technology environments. With the advent of long-duration space flight, group-related issues of crew functioning will take on added significance. This paper discusses the influence of crew roles, status, leadership, and norms on the performance of small, confined groups, and offers guidelines and suggestions regarding organizational design, crew selection, training, and team building for crew productivity and social well-being in long-duration spaceflight.

Nicholas, John M.↗

A prototype case-based reasoning human assistant for space crew assessment and mission management

We present a prototype human assistant system for space crew assessment and mission management. Our system is based on case episodes from American and Russian space missions and analog environments such as polar stations and undersea habitats. The general domain of small groups in isolated and confined environments represents a near ideal application area for case-based reasoning (CBR) - there are few reliable rules to follow, and most domain knowledge is in the form of cases. We define the problem domain and outline a unique knowledge representation system driven by conflict and communication triggers. The prototype system is able to represent, index, and retrieve case studies of human performance. We index by social, behavioral, and environmental factors. We present the problem domain, our current implementation, our research approach for an operational system, and prototype performance and results.

Owen, Robert B.↗

Integrating CLIPS applications into heterogeneous distributed systems

SOCIAL is an advanced, object-oriented development tool for integrating intelligent and conventional applications across heterogeneous hardware and software platforms. SOCIAL defines a family of 'wrapper' objects called agents, which incorporate predefined capabilities for distributed communication and control. Developers embed applications within agents and establish interactions between distributed agents via non-intrusive message-based interfaces. This paper describes a predefined SOCIAL agent that is specialized for integrating C Language Integrated Production System (CLIPS)-based applications. The agent's high-level Application Programming Interface supports bidirectional flow of data, knowledge, and commands to other agents, enabling CLIPS applications to initiate interactions autonomously, and respond to requests and results from heterogeneous remote systems. The design and operation of CLIPS agents are illustrated with two distributed applications that integrate CLIPS-based expert systems with other intelligent systems for isolating and mapping problems in the Space Shuttle Launch Processing System at the NASA Kennedy Space Center.

Adler, Richard M.↗

Shifting institutional culture to develop climate solutions with Open Science

This call to action by Drs. Johnson and Wilkinson is part of a mosaic of voices sharing tangible progress within the climate movement 1,2. This call speaks to us as environmental and Earth scientists motivated by the urgency of climate change and social inequity and who contribute to finding science-driven climate solutions as part of our daily jobs. Unfortunately, we are often unable to efficiently move this critical and urgent work forward because we are impeded by cumbersome daily workflows and restrictive workplace cultures. Our workplaces have not kept pace with the modern realities of data-intensive science: increasing data volumes and storage needs, rapidly evolving technology, new skill requirements, and a growing need for extensive and diverse collaboration. Struggling with old approaches and learning new ones in isolation can fuel burnout and turnover, preventing us from working on science-driven climate solutions effectively.

open science↗

Space in Space: Designing for Privacy in the Workplace

Privacy is cultural, socially embedded in the spatial, temporal, and material aspects of the lived experience. Definitions of privacy are as varied among scholars as they are among those who fight for their personal rights in the home and the workplace. Privacy in the workplace has become a topic of interest in recent years, as evident in discussions on Big Data as well as the shrinking office spaces in which people work. An article in The New York Times published in February of this year noted that "many companies are looking to cut costs, and one way to do that is by trimming personal space". Increasingly, organizations ranging from tech start-ups to large corporations are downsizing square footage and opting for open-office floorplans hoping to trim the budget and spark creative, productive communication among their employees. The question of how much is too much to trim when it comes to privacy, is one that is being actively addressed by the National Aeronautics and Space Administration (NASA) as they explore habitat designs for future space missions. NASA recognizes privacy as a design-related stressor impacting human health and performance. Given the challenges of sustaining life in an isolated, confined, and extreme environment such as Mars, NASA deems it necessary to determine the acceptable minimal amount for habitable volume for activities requiring at least some level of privacy in order to support optimal crew performance. Ethnographic research was conducted in 2013 to explore perceptions of privacy and privacy needs among astronauts living and working in space as part of a long-distance, long-duration mission. The allocation of space, or habitable volume, becomes an increasingly complex issue in outer space due to the costs associated with maintaining an artificial, confined environment bounded by limitations of mass while located in an extreme environment. Privacy in space, or space in space, provides a unique case study of the complex notions of privacy, the impact of design and others on achieving it, and the sensemaking that occurs when privacy is less than expected. The findings show that privacy is not just a personal, individual need but is also a need that is shared among teams and groups. Moreover, the case of space in space reveals the influence the design of the built and social environments have on privacy needs and on achieving privacy. When the level of privacy is less than expected, sensemaking occurs and the lack of privacy is dealt with by means of absencing the present. creating new social norms, and "making space" by manipulating the spatial, temporal, material aspects of the lived experience. Although the Mars habitat study represents an extreme case of privacy in the workplace, lessons learned from outer space are applicable to life in the Earth-bound workplace. A mini-case study was conducted to evaluate office space at the headquarters of a major American airline that illustrates the usefulness of building unexpected bridges between the unknown, unfamiliar Mars habitat and the everyday workplace. The comparative studies reveal insight into the interconnected, social nature of the spatial, temporal, and material aspects of the lived experience and how users of the habitat and office workspace view privacy, self, and others through an embodied, design interaction.

Akin, Jonie↗

Human Adaptation to Isolated and Confined Environments

A study was conducted over seven months in a winter Antarctic isolated and confined environment (ICE). Physiological and psychological data was collected several times a week. Information was collected on a monthly basis on behavior and the use of physical facilities. Adaptation and information indicated that there was a significant decrease in epinephrine and norepinephrine during the middle trimester of the winter. No vital changes were found for blood pressure. Self reports of hostility and anxiety show a linear increase. There were no significant changes in depression during ICE. The physiological and psychological data do not move in a synchronous fashion over time. The data also suggest that both ambient qualities of an ICE and discrete social environmental events, such as the arrival of the summer crew, have an impact on the outcome measures used. It may be most appropiate to develop a model for ICE's that incorporates not only global chronic stressors common to all ICE's but also the role of discrete environmental effects which can minimize or enhance the influence of more chronic stressors. Behavioral adjustment information highlight the importance of developing schedules which balance work and recreational activities.

Evans, Gary W.↗

Advances in Rodent Research Missions on the International Space Station

A Research platform for rodent experiment on the ISS is an essential tool for advancing biomedical research in space. The Rodent Research allows for experiments of much longer duration that experiments on the Shuttle. NASA’s Rodent Research (RR)-1 mission was successfully completed, including post-flight analysis and achieved a number of objectives including validation of flight hardware, on-orbit operations, and science capabilities that were developed at the NASA Ames Research Center. Briefly, twenty C57BL/6J adult female mice were launched on the SpX4 Dragon vehicle, which thrived for up to 37 days in microgravity. Daily health checks of the mice were performed during the mission via downlinked video; all animals were healthy and displayed normal behavior without any significant signs of stress. Behavioral analysis demonstrated that Flight and Ground Control mice exhibited the same range of behaviors, including eating, drinking, exploratory behavior, self- and allo-grooming, and social interactions indicative of healthy animals. The animals were euthanized and select tissues were collected from some of the mice on orbit to assess the long-term sample storage capabilities of the ISS. The data obtained from the flight mice were comparable to those from the 3 groups of control mice (baseline, vivarium and ground controls), suggesting that the ISS has adequate capability to support long-duration rodent experimentations. We recovered over 35 tissues from 40 RR1 frozen carcasses, yielded over 3200 aliquots of tissues, and distributed to the scientific community, including NASA’s GeneLab and scientists in the U.S. through Biospecimen Sharing Program via Ames Life Science Data Archive. Tissues were also distributed to Russian research colleagues at the Institute for Biomedical Problems. The expression levels of select genes including albumin, catalase, GAPDH, HMGCoA Reductase, and IGF1 were determined using RNA isolated from the livers by qPCR and no significant differences by one factor ANOVA were found between flight and ground control groups. In addition, some of the liver samples were subject to transcriptomics, epigenomics and proteomics. The data are now available to the scientific community through GeneLab’s open science data website. Since the RR1 mission, another long duration mission (Rodent Research-2) was completed on the ISS in 2015 in which 20 female C57 BL/6J mice were successfully maintained on the ISS for varying time points, with the last group of 5 animals being on-orbit for 54 days. This second Rodent Research flight expanded the program’s capabilities with the introduction of new technologies including blood collection and separation and bone densitometry scanning. Furthermore, we have continued to expand the ISS’s capabilities by running a series of ground-based verification testing using male mice. Our next step is to fly male mice for Rodent Research-4 on SpaceX-10 to study the effects of microgravity on bone healing and regeneration. It will be the first long-duration mission using male mice using Rodent Hardware. In addition, the number of mice will increase from 20 mice (on RR-1 and RR-2) to 40 for RR-4. When samples return to Earth, a number of tissues will be dissected from the frozen carcasses and select tissue samples will become available to the scientific community via BSP. Altogether, we have continued to expand our capabilities for performing long-duration missions on the ISS as emphasized in the National Research Council’s Decadal Survey released in 2011 and to maximize science return from each mission.

Choi, S.↗

Enabling Innovation and Collaboration Across Geography and Culture: A Case Study of NASA's Systems Engineering Community of Practice

In 2004, NASA faced major knowledge sharing challenges due to geographically isolated field centers that inhibited personnel from sharing experiences and ideas. Mission failures and new directions for the agency demanded better collaborative tools. In addition, with the push to send astronauts back to the moon and to Mars, NASA recognized that systems engineering would have to improve across the agency. Of the ten field centers, seven had not built a spacecraft in over 30 years, and had lost systems engineering expertise. The Systems Engineering Community of Practice came together to capture the knowledge of its members using the suite of collaborative tools provided by the NASA Engineering Network (NEN.) The NEN provided a secure collaboration space for over 60 practitioners across the agency to assemble and review a NASA systems engineering handbook. Once the handbook was complete, they used the open community area to disseminate it. This case study explores both the technology and the social networking that made the community possible, describes technological approaches that facilitated rapid setup and low maintenance, provides best practices that other organizations could adopt, and discusses the vision for how this community will continue to collaborate across the field centers to benefit the agency as it continues exploring the solar system.

NEN↗

Critical Team Composition Issues for Long-Distance and Long-Duration Space Exploration: A Literature Review, an Operational Assessment, and Recommendations for Practice and Research

Prevailing team effectiveness models suggest that teams are best positioned for success when certain enabling conditions are in place (Hackman, 1987; Hackman, 2012; Mathieu, Maynard, Rapp, & Gilson, 2008; Wageman, Hackman, & Lehman, 2005). Team composition, or the configuration of member attributes, is an enabling structure key to fostering competent teamwork (Hackman, 2002; Wageman et al., 2005). A vast body of research supports the importance of team composition in team design (Bell, 2007). For example, team composition is empirically linked to outcomes such as cooperation (Eby & Dobbins, 1997), social integration (Harrison, Price, Gavin, & Florey, 2002), shared cognition (Fisher, Bell, Dierdorff, & Belohlav, 2012), information sharing (Randall, Resick, & DeChurch, 2011), adaptability (LePine, 2005), and team performance (e.g., Bell, 2007). As such, NASA has identified team composition as a potentially powerful means for mitigating the risk of performance decrements due to inadequate crew cooperation, coordination, communication, and psychosocial adaptation in future space exploration missions. Much of what is known about effective team composition is drawn from research conducted in conventional workplaces (e.g., corporate offices, production plants). Quantitative reviews of the team composition literature (e.g., Bell, 2007; Bell, Villado, Lukasik, Belau, & Briggs, 2011) are based primarily on traditional teams. Less is known about how composition affects teams operating in extreme environments such as those that will be experienced by crews of future space exploration missions. For example, long-distance and long-duration space exploration (LDSE) crews are expected to live and work in isolated and confined environments (ICEs) for up to 30 months. Crews will also experience communication time delays from mission control, which will require crews to work more autonomously (see Appendix A for more detailed information regarding the LDSE context). Given the unique context within which LDSE crews will operate, NASA identified both a gap in knowledge related to the effective composition of autonomous, LDSE crews, and the need to identify psychological and psychosocial factors, measures, and combinations thereof that can be used to compose highly effective crews (Team Gap 8). As an initial step to address Team Gap 8, we conducted a focused literature review and operational assessment related to team composition issues for LDSE. The objectives of our research were to: (1) identify critical team composition issues and their effects on team functioning in LDSE-analogous environments with a focus on key composition factors that will most likely have the strongest influence on team performance and well-being, and 1 Astronaut diary entry in regards to group interaction aboard the ISS (p.22; Stuster, 2010) 2 (2) identify and evaluate methods used to compose teams with a focus on methods used in analogous environments. The remainder of the report includes the following components: (a) literature review methodology, (b) review of team composition theory and research, (c) methods for composing teams, (d) operational assessment results, and (e) recommendations.

Bell, Suzanne T.↗

Advances in Rodent Research Missions on the International Space Station

A research platform for rodent experiment on the ISS is a valuable tool for advancing biomedical research in space. Capabilities offered by the Rodent Research project developed at NASA Ames Research Center can support experiments of much longer duration on the ISS than previous experiments performed on the Space Shuttle. NASAs Rodent Research (RR)-1 mission was completed successfully and achieved a number of objectives, including validation of flight hardware, on-orbit operations, and science capabilities as well as support of a CASIS-sponsored experiment (Novartis) on muscle atrophy. Twenty C57BL6J adult female mice were launched on the Space-X (SpX) 4 Dragon vehicle, and thrived for up to 37 days in microgravity. Daily health checks of the mice were performed during the mission via downlinked video; all flight animals were healthy and displayed normal behavior, and higher levels of physical activity compared to ground controls. Behavioral analysis demonstrated that Flight and Ground Control mice exhibited the same range of behaviors, including eating, drinking, exploratory behavior, self- and allo-grooming, and social interactions indicative of healthy animals. The animals were euthanized on-orbit and select tissues were collected from some of the mice on orbit to assess the long-term sample storage capabilities of the ISS. In general, the data obtained from the flight mice were comparable to those from the three groups of control mice (baseline, vivarium and ground controls, which were housed in flight hardware), showing that the ISS has adequate capability to support long-duration rodent experiments. The team recovered 35 tissues from 40 RR-1 frozen carcasses, yielding 3300 aliquots of tissues to distribute to the scientific community in the U.S., including NASAs GeneLab project and scientists via Space Biology's Biospecimen Sharing Program Ames Life Science Data Archive. Tissues also were distributed to Russian research colleagues at the Institute for Biomedical Problems. The expression levels of select genes including albumin, catalase, GAPDH, HMGCoA Reductase, and IGF1 were determined using RNA isolated from the livers by qPCR and no significant differences by one factor ANOVA were found between flight and ground control groups. In addition, some of the liver samples were analyzed for transcriptomic, epigenomic and proteomic profiles; some of the data sets are now available to the scientific community through GeneLabs open science data website. A second long duration mission, Rodent Research-2 (RR-2) was completed on the ISS in 2015; 20 female C57BL6J mice were successfully maintained on the ISS for various durations, with the last group of 5 animals living on-orbit for 54 days. Furthermore, we continue to expand the ISSs capabilities by introducing new on-orbit technologies including blood collection and separation, bone densitometry scanning, muscle grip strength and anesthesia with recovery. In addition, series of ground-based verification testing to fly male mice and increase the total number of mice on-orbit from 20 to 40. Subsequent missions will provide the capability to return live mice from the ISS animals to evaluate recovery on Earth, further expanding operational and science capabilities of the RR project on the ISS.

Choi, S. Y.↗

Human behavioral research in space: quandaries for research subjects and researchers

With the advent of long-duration spaceflight on board the International Space Station (ISS) and possible future missions beyond low Earth orbit (LEO) such as Mars, it is critical that those at NASA have a realistic assessment of the challenges that will face individuals on long-duration missions so that they can develop preventive and real-time countermeasures to behavioral health issues. While space travellers are very interested in having countermeasures to the deleterious effects of space missions, they have a powerful disincentive to participate in this research if they feel it could in any way negatively affect their flight status. The behavioral issues of isolation and confinement for extended-duration space missions are reviewed. Areas of basic and clinical behavioral research are listed. And the classical clinical model for research is not considered appropriate for the current configuration of the space program. The use of analogue environments and advanced statistical analysis are suggested as ways to address the limited spaceflight research opportunities. The challenge of research subject or patient confidentiality vs. mission safety and issues of personal flight status are addressed.

Review, Tutorial↗

Space analogue studies in Antarctica

Medical research has been carried out on the Australian National Antarctic Research Expeditions (ANARE) for 50 years. As an extension of this program collaborative Australian/United States research on immunology, microbiology, psychology and remote medicine has produced important data and insight on how humans adapt to the stress of extreme isolation, confinement and the harsh environment of Antarctica. An outstanding analogue for the isolation and confinement of space missions (especially planetary outposts), ANARE has been used as an international research platform by Australia and the United States since 1993. Collaborative research has demonstrated a lowered responsiveness of the immune system under the isolation and confinement of Antarctic winter-over; a reduction of almost 50% in T cell proliferation to mitogen phytohaemogglutinin, as well as changes in latent herpesvirus states and the expansion of the polyclonal latent Epstein-Barr virus infected B cell populations. Although no clinically significant disease has been found to result from these immune changes, research is currently assessing the effects of psychological factors on the immune system. This and associated research performed to date and its relevance to both organisations is discussed, and comment made on possible extensions to the program in both medical and other fields.

NASA Center HQS↗

Implications of privacy needs and interpersonal distancing mechanisms for space station design

Isolation, confinement, and the characteristics of microgravity will accentuate the need for privacy in the proposed NASA space station, yet limit the mechanism available for achieving it. This study proposes a quantitative model for understanding privacy, interpersonal distancing, and performance, and discusses the practical implications for Space Station design. A review of the relevant literature provided the basis for a database, definitions of physical and psychological distancing, loneliness, and crowding, and a quantitative model of situational privacy. The model defines situational privacy (the match between environment and task), and focuses on interpersonal contact along visual, auditory, olfactory, and tactile dimensions. It involves summing across pairs of crew members, contact dimensions, and time, yet also permits separate analyses of subsets of crew members and contact dimensions. The study concludes that performance will benefit when the type and level of contact afforded by the environment align with that required by the task. The key to achieving this is to design a flexible, definable, and redefinable interior environment that provides occupants with a wide array of options to meet their needs for solitude, limited social interaction, and open group activity. The report presents 49 recommendations in five categories to promote a wide range of privacy options despite the space station's volumetric limitations.

Harrison, Albert A.↗

Measuring Unique Contextual Factors: Group Living Skills

BACKGROUND The Human Factors and Behavioral Performance Exploration Measures (HFBP-EM) suite is a set of standardized measures used to assess behavioral health and performance risks related to future exploration-class space missions. During the HFBP-EM suite development, the importance of assessing unique contextual factors to understand the drivers of team dynamics was identified. Group living skills are a unique workplace skill which applies to those coworkers who live and work together. As mission lengths become longer, the line between work and non-work life may blur. Extreme living conditions such as spaceflight can result in increased stress over time, negatively impact mood and well-being, decrease team cohesion and increase conflict, deteriorate task performance, and potentially reduce mission success [1]. With no breaks in their isolated and confined environment possible, living with a messy, inconsiderate crewmember can add to the stress. Alternatively, crews with good group living skills may support one another, mitigating the negative effects of the extreme environment. There was no direct measure of group living skills. This work sought to fill this measurement gap and collect data related to group living in isolated, confined, extreme (ICE) environments. METHODS Group living skills as an astronaut competency area were developed with the input of experts familiar with ICE environments. Distinct aspects of this competency were reviewed and discussed by a NASA-invited group of spaceflight experts in 2015 to identify individual and team behavioral health and performance measures for operational environments. This work resulted in a 5-item measure focused on group living experiences with each crewmember (or the crew as a whole). A 6th item asks whether an individual would go on another mission with a particular crewmember or crew as a measure of future-oriented group living, or team viability. The measure was deployed in 24 teams living and working together in isolated and confined environments (e.g., International Space Station, space mission simulation analogs). RESULTS & DISCUSSION In this presentation, we will summarize results which indicate the Group Living Skills (GLS) Survey is a reliable, valid, and operationally feasible measure. Reliability was evaluated in several ways. Two-way multilevel mixed models revealed strong repeated measures reliability (ICC2 = .902, Omega between = .954). Reliability of change within teams was strong (Rc = .81). Factor analysis showed a consistent two-factor structure when individuals rated either the whole crew, all individuals, just peers, or a focal roommate. The latter used a sociometric approach to rating the GLS of the roommates. The two factors were tidiness and being considerate of others. We assessed the criterion-related validity of GLS, by examining GLS operationalized as the team-level aggregated GLS Survey scores as a predictor of team viability, team cohesion, and team performance. We used generalized mixed models to account for the repeated measures and longitudinal data. The marginal R2 change was used to determine the extent to which GLS were related to the team outcomes while controlling for mission day and campaign. Results suggested GLS was strongly related to team viability (marginal R2 change = .40), team cohesion (marginal R2 change = 0.28), social cohesion (marginal R2 change = 0.25), and moderately related to task cohesion (marginal R2 change = 0.18). GLS scores had a small relationship with team performance (marginal R2 change = .08). Results support GLS as a measure that can be used to capture group living skills of crews who live and work together in ICE. Because the items were written to be broadly applicable, less extreme environments with teams living and working together for some length of time also benefit from this measure (e.g., college roommates, camps). See Landon et al. (2024) for full results [2]. SUMMARY We report reliability and validity evidence for a new measure that assesses the Group Living Skills for teams that live and work in operational environments such as spaceflight.

J C W Miller↗

Space Crop Production Gaps and Challenges

As astronauts venture farther from Earth, and stay for longer periods, the space food system will increase in importance. Crop production can supplement a pre-packaged space diet to provide nutrition and dietary variety for space crews. In future missions, bioregenerative approaches may be used to generate a larger percentage of the diet, as well as help to reduce life support system burdens and resupply from Earth. Plants may also provide behavioral health benefits to crew members living in the isolated, confined environment of a space habitat. A number of unique challenges exist for growth of plants in microgravity and on other reduced gravity surfaces like the moon and Mars. Testing plant growth inside the Veggie and Advanced Plant Habitat (APH) chambers on the International Space Station is allowing us to understand the impacts of gravity and spaceflight on crop growth, nutritional content, acceptability, and the importance of plants to astronauts living and working away from Earth. We are also gaining a better understanding of food safety concerns and the behavior of space plant microbiomes and plant pathogens, but major gaps in knowledge remain. As we move from research towards operational space crop production to enable exploration, there are numerous gaps in technology, knowledge, and practice related to space crop growth that must be addressed. Research and development in key focus areas such as effective water and nutrient delivery at variable gravity levels, autonomous plant health monitoring, growth system cleaning and disinfection, and selection of ideal space crops are needed to fill these gaps. Breeding or engineering custom space crops may impact areas including plant growth and development, plant physiology, produce nutrition, organoleptic acceptability, and post-harvest characteristics, and these may further enable space crop production scenarios. Space crop challenges are multifaceted and require diverse interdisciplinary teams working together to develop effective solutions. Solving these requires an array of skill sets from across the biological and physical sciences, engineering, and human social sciences. Solutions to help ensure food security off-Earth may also translate to more sustainable terrestrial crop production approaches, and regular dialog between industry, academia, and government organizations working in related fields benefit all. Additional help can come from engagement with student researchers at various levels through courses, participatory science projects, and open science activities which can provide useful data. Global coordination and integration between space agencies and partners will be essential.

Gioia Donna Massa↗

Space Crop Production Gaps and Challenges

As astronauts venture farther from Earth, and stay for longer periods, the space food system will increase in importance. Crop production can supplement a pre-packaged space diet to provide nutrition and dietary variety for space crews. In future missions, bioregenerative approaches may be used to generate a larger percentage of the diet, as well as help to reduce life support system burdens and resupply from Earth. Plants may also provide behavioral health benefits to crew members living in the isolated, confined environment of a space habitat. A number of unique challenges exist for growth of plants in microgravity and on other reduced gravity surfaces like the moon and Mars. Testing plant growth inside the Veggie and Advanced Plant Habitat (APH) chambers on the International Space Station is allowing us to understand the impacts of gravity and spaceflight on crop growth, nutritional content, acceptability, and the importance of plants to astronauts living and working away from Earth. We are also gaining a better understanding of food safety concerns and the behavior of space plant microbiomes and plant pathogens, but major gaps in knowledge remain. As we move from research towards operational space crop production to enable exploration, there are numerous gaps in technology, knowledge, and practice related to space crop growth that must be addressed. Research and development in key focus areas such as effective water and nutrient delivery at variable gravity levels, autonomous plant health monitoring, growth system cleaning and disinfection, and selection of ideal space crops are needed to fill these gaps. Breeding or engineering custom space crops may impact areas including plant growth and development, plant physiology, produce nutrition, organoleptic acceptability, and post-harvest characteristics, and these may further enable space crop production scenarios. Space crop challenges are multifaceted and require diverse interdisciplinary teams working together to develop effective solutions. Solving these requires an array of skill sets from across the biological and physical sciences, engineering, and human social sciences. Solutions to help ensure food security off-Earth may also translate to more sustainable terrestrial crop production approaches, and regular dialog between industry, academia, and government organizations working in related fields benefit all. Additional help can come from engagement with student researchers at various levels through courses, participatory science projects, and open science activities which can provide useful data. Global coordination and integration between space agencies and partners will be essential.

Gioia Massa↗

Flashline Mars Arctic Research Station (FMARS) 2009 Expedition Crew Perspectives

The Flashline Mars Arctic Research Station (FMARS), located on the rim of the Haughton Crater on Devon Island in the Canadian Arctic, is a simulated Mars habitat that provides operational constraints similar to those which will be faced by future human explorers on Mars. In July 2009, a six-member crew inhabited the isolated habitation module and conducted the twelfth FMARS mission. The crew members conducted frequent EVA operations wearing mock space suits to conduct field experiments under realistic Mars-like conditions. Their scientific campaign spanned a wide range of disciplines and included many firsts for Mars analog research. Among these are the first use of a Class IV medical laser during a Mars simulation, helping to relieve crew stress injuries during the mission. Also employed for the first time in a Mars simulation at FMARS, a UAV (Unmanned Aerial Vehicle) was used by the space-suited explorers, aiding them in their search for mineral resources. Sites identified by the UAV were then visited by geologists who conducted physical geologic sampling. For the first time, explorers in spacesuits deployed passive seismic equipment to monitor earthquake activity and characterize the planet's interior. They also conducted the first geophysical electromagnetic survey as analog Mars pioneers to search for water and characterize geological features under the surface. The crew collected hydrated minerals and attempted to produce drinkable water from the rocks. A variety of equipment was field tested as well, including new cameras that automatically geotag photos, data-recording GPS units, a tele-presence rover (operated from Florida), as well as MIT-developed mission planning software. As plans develop to return to the Moon and go on to Mars, analog facilities like FMARS can provide significant benefit to NASA and other organizations as they prepare for robust human space exploration. The authors will present preliminary results from these studies as well as their perspectives on topics including human factors, logistics, EVA operations, and the use of social media throughout the mission.

Cusack, Stacy↗