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At least 91 records · Page 5

Spatial and temporal traction response in human airway smooth muscle cells

Tractions that cells exert on their substrates are essential in cell spreading, migration, and contraction. These tractions can be determined by plating the cells on a flexible gel and measuring the deformation of the gel by using fluorescent beads embedded just below the surface of the gel. In this article we describe the image correlation method (ICM) optimized for determining the displacement field of the gel under a contracting cell. For the calculation of the traction field from the displacement field we use the recently developed method of Fourier transform traction cytometry (FTTC). The ICM and FTTC methods are applied to human airway smooth muscle cells during stimulation with the contractile agonist histamine or the relaxing agonist isoproterenol. The overall intensity of the cell contraction (the median traction magnitude, the energy transferred from the cell to the gel, and the net contractile moment) increased after activation with histamine, and decreased after treatment with isoproterenol. Cells exhibited regional differences in the time course of traction during the treatment. Both temporal evolution and magnitude of traction increase induced by histamine varied markedly among different cell protrusions, whereas the nuclear region showed the smallest response. These results suggest that intracellular mediators of cell adhesion and contraction respond to contractile stimuli with different rates and intensities in different regions of the cell.

NASA Discipline Cell Biology↗

Crater Flux Transfer Events: Highroad to the X Line?

We examine Cluster observations of a so-called magnetosphere crater FTE, employing data from five instruments (FGM, CIS, EDI, EFW, and WHISPER), some at the highest resolution. The aim of doing this is to deepen our understanding of the reconnection nature of these events by applying recent advances in the theory of collisionless reconnection and in detailed observational work. Our data support the hypothesis of a stratified structure with regions which we show to be spatial structures. We support the bulge-like topology of the core region (R3) made up of plasma jetting transverse to reconnected field lines. We document encounters with a magnetic separatrix as a thin layer embedded in the region (R2) just outside the bulge, where the speed of the protons flowing approximately parallel to the field maximizes: (1) short (fraction of a sec) bursts of enhanced electric field strengths (up to approximately 30 mV/m) and (2) electrons flowing against the field toward the X line at approximately the same time as the bursts of intense electric fields. R2 also contains a density decrease concomitant with an enhanced magnetic field strength. At its interface with the core region, R3, electric field activity ceases abruptly. The accelerated plasma flow profile has a catenary shape consisting of beams parallel to the field in R2 close to the R2/R3 boundary and slower jets moving across the magnetic field within the bulge region. We detail commonalities our observations of crater FTEs have with reconnection structures in other scenarios. We suggest that in view of these properties and their frequency of occurrence, crater FTEs are ideal places to study processes at the separatrices, key regions in magnetic reconnection. This is a good preparation for the MMS mission.

Farrugia, C. J.↗

Direct Solar Wind Proton Access into Permanently Shadowed Lunar Polar Craters

Recent analyses of Lunar Prospector neutron spectrometer (LPNS) data have suggested that high abundances of hydrogen exist within cold traps at the lunar poles, and it has often been assumed that hydrogen-bearing volatiles sequestered in permanent shadow are topographically shielded from sputtering by solar wind protons. However, recent simulation results are presented showing that solar wind protons clearly access the floor of an idealized, shadowed lunar crater through a combination of thermal and ambipolar processes, in effect creating a plasma "miniwake". These simulations are the first to model the mini-wake environment in two spatial dimensions with a self-consistent lunar surface-plasma interaction. Progress is reported on constraining the nonzero particle fluxes and energies incident on kilometer-scale shadowed topography, such as a small crater embedded within a larger one. The importance of direct solar wind proton bombardment is discussed within the context of understanding the stability and inventory of hydrogen-bearing volatiles in shadow at the lunar poles. The support of the National Lunar Science institute, the DREAM institute, LPROPS, and the NASA Postdoctoral Program at NASA Goddard Space Flight Center administered by ORAU are gratefully acknowledged.

Zimmerman, M. I.↗

Multiscale Studies of the Three-Dimensional Dayside X-Line

We review recent experience from the Cluster, Double Star, and THEMIS missions for lessons that apply to the upcoming Magnetospheric Multiscale Mission (MMS) being developed for launch in 2014. On global scales, simulation and statistical studies lead to mean congurations of dayside reconnection, implying specific relative alignments of the inflow magnetic fields and X-line, with implications for MMS operations designed to maximize the number of close encounters with the diffusion region. At intermediate MHD-to-ion scales, reconstruction of features created by one or two X-lines have developed to the point where data from a cluster of spacecraft can determine their temporal trends and the approximate three-dimensional X-line structure. Recent petascale particle-in-cell (PIC) simulations of reconnection encompass three spatial dimensions with excellent resolution, and make striking predictions of electron scale physics that creates complex interacting flux ropes under component reconnection. High time resolution measurements from MMS will determine the detailed electron scale kinetics embedded within the global and MHDion scale contexts. These developments will lead to the refinement of our three-dimensional multiscale picture of reconnection, yielding improved understanding of the global, MHD, and local physics controlling the onset or quenching, variability, and mean rate of reconnection. This in turn will enable improved predictability of the structural features created by transient reconnection, and their space weather consequences.

Reconnection↗

Indigenous Organic Matter Associated with Unusual Low Temperature Aqueous Secondary Phases in the Mars Meteorite Nakhla

In freshly fractured chips of the Mars meteorite Nakhla, we found carbonaceous phases intimately associated with secondary aqueous alteration phases, both mineral and amorphous, interpreted to have formed through the low-temperature, mineral-water dissolution of the host Martian basalt.We have characterized the nature of carbonaceous matter and spatially associated secondary phases within Nakhla using a multidisciplinary suite of optical and electron beam instrumentation(Thomas-Keprta et al., 2022). Secondary Phases: The preserved secondary mineral phases are best described as ‘juvenile’ hydrated amorphous silica formed when water interacts with mafic rocks. Although amorphous silica on Earth rarely persists through geologic time due to alteration into other phases, e.g.,microcrystalline opal-CT and eventually quartz, the lack of maturation of primarily amorphous silica with embedded nano phase ferrihydrite in Nakhla infers these phases formed in a limited water environment by low temperature, circumneutral pH fluids stabilized by the presence of coexisting amorphous silica.Additionally, we report the presence of the ferrous hydroxycarbonate mineral chukanovite (Fe2(OH)2CO3)within some of the secondary mineral aggregates studied. Organic Matter:Indigenous Nakhla carbonaceous matter is present both in condensed phases and in a dispersed state spatially associated with secondary alteration phases. In the former, discrete refractory micron to submicron assemblages were identified that appear macromolecular in nature and, in several cases, associated with F and, in one case, significant N. While organically bound Fhas yet to be detected remotely on Mars, the identification of organic N within one of the carbonaceous phases is considered a prerequisite for Martian astrobiological potential. Summary: Neither the intimate association of carbonaceous matter with secondary phases nor the identification of chukanovite have been previously reported in any of the Martian meteorites.A variety of habitability-related metastable solid-phase, oxidation-reduction, and organic-molecular sample attributes in Nakhla formed hundreds of millions of years ago near Mars’ surface; these alteration products persisted on Mars until very recentlyand resemble the amorphous materials observed at Gale crater by Mars Science Laboratory CuriosityRover (Rampe et al., 2020).Similar phases may be expected to occur elsewhere on Mars (e.g., Jezerocrater). References: Rampe E. B. et al.(2020) Mineralogy and geochemistry of sedimentary rocks and eolian sediments in Gale crater, Mars: A review after six Earth years of exploration with Curiosity.Geochemistry80, 125605; Thomas-Keprta K.L. et al. (2022) Indigenous carbon-rich matter associated with unusual aqueous alteration features in Nakhla: Complex formation and preservation history. Geochim. et Cosmochim. Acta320, 41-78 (online).

Mars meteorite↗

Measurement of Separated Flow Structures Using a Multiple-Camera DPIV System

A novel multiple-camera system for the recording of digital particle image velocimetry (DPIV) images acquired in a two-dimensional separating/reattaching flow is described. The measurements were performed in the NASA Langley Subsonic Basic Research Tunnel as part of an overall series of experiments involving the simultaneous acquisition of dynamic surface pressures and off-body velocities. The DPIV system utilized two frequency-doubled Nd:YAG lasers to generate two coplanar, orthogonally polarized light sheets directed upstream along the horizontal centerline of the test model. A recording system containing two pairs of matched high resolution, 8-bit cameras was used to separate and capture images of illuminated tracer particles embedded in the flow field. Background image subtraction was used to reduce undesirable flare light emanating from the surface of the model, and custom pixel alignment algorithms were employed to provide accurate registration among the various cameras. Spatial cross correlation analysis with median filter validation was used to determine the instantaneous velocity structure in the separating/reattaching flow region illuminated by the laser light sheets. In operation the DPIV system exhibited a good ability to resolve large-scale separated flow structures with acceptable accuracy over the extended field of view of the cameras. The recording system design provided enhanced performance versus traditional DPIV systems by allowing a variety of standard and non-standard cameras to be easily incorporated into the system.

Humphreys, William M., Jr.↗

X-rays from radio pulsars - The detection of PSR 1055-52

The short-period pulsar PSR 1055-52 has been detected as a soft X-ray source in the course of an Einstein Observatory survey of radio pulsars. Its X-ray to radio luminosity ratio is about 10,000, although the X-rays are not modulated at the neutron star's rotation frequency. High spatial resolution observations suggest that a significant fraction of the emission comes from an extended region surrounding the pulsar. Several possible scenarios for the origin of both point and extended X-ray emission from isolated neutron stars are investigated: radiation from the hot stellar surface, from hot polar caps, and from an optically thick atmosphere, as well as from a circumstellar nebula emitting thermal bremsstrahlung or synchrotron radiation. It is concluded that the spatial, spectral, and temporal characteristics of this source are most consistent with a model in which relativistic particles generated by the pulsar are radiating synchrotron X-rays in the surrounding magnetic field; i.e., that PSR 1055 is embedded in a mini-Crab nebula. Observational tests of this hypothesis are suggested, and the implications of this result for pulsar evolution are briefly discussed.

Cheng, A. F.↗

How Very Massive Metal-Free Stars Start Cosmological Reionization

The initial conditions and relevant physics for the formation of the earliest galaxies are well specified in the concordance cosmology. Using ab initio cosmological Eulerian adaptive mesh refinement radiation hydrodynamical calculations, we discuss how very massive stars start the process of cosmological reionization. The models include nonequilibrium primordial gas chemistry and cooling processes and accurate radiation transport in the case B approximation using adaptively ray-traced photon packages, retaining the time derivative in the transport equation. Supernova feedback is modeled by thermal explosions triggered at parsec scales. All calculations resolve the local Jeans length by at least 16 grid cells at all times and as such cover a spatial dynamic range of approx.10(exp 6). These first sources of reionization are highly intermittent and anisotropic and first photoionize the small-scale voids surrounding the halos they form in, rather than the dense filaments they are embedded in. As the merging objects form larger, dwarf-sized galaxies, the escape fraction of UV radiation decreases and the H II regions only break out on some sides of the galaxies, making them even more anisotropic. In three cases, SN blast waves induce star formation in overdense regions that were formed earlier from ionization front instabilities. These stars form tens of parsecs away from the center of their parent DM halo. Approximately five ionizing photons are needed per sustained ionization when star formation in 10(exp 6) stellar Mass halos is dominant in the calculation. As the halos become larger than approx.10(exp 7) Stellar Mass, the ionizing photon escape fraction decreases, which in turn increases the number of photons per ionization to 15-50, in calculations with stellar feedback only. Radiative feedback decreases clumping factors by 25% when compared to simulations without star formation and increases the average temperature of ionized gas to values between 3000 and 10,000 K.

Wise, John H.↗

Preconditioning for the Navier-Stokes equations with finite-rate chemistry

The preconditioning procedure for generalized finite-rate chemistry and the proper preconditioning for the one-dimensional Navier-Stokes equations are presented. Eigenvalue stiffness is resolved and convergence-rate acceleration is demonstrated over the entire Mach-number range from the incompressible to the hypersonic. Specific benefits are realized at low and transonic flow speeds. The extended preconditioning matrix accounts for thermal and chemical non-equilibrium and its implementation is explained for both explicit and implicit time marching. The effect of higher-order spatial accuracy and various flux splittings is investigated. Numerical analysis reveals the possible theoretical improvements from using proconditioning at all Mach numbers. Numerical results confirm the expectations from the numerical analysis. Representative test cases include flows with previously troublesome embedded high-condition-number regions.

Godfrey, Andrew G.↗

Chloroplast Ultrastructure of the Alga Phaeocystis antarctica Karsten: A New Structural Model Using Electron Tomography

Understanding the light-harvesting properties of algae and higher plants are a fundamental topic in photosynthesis research. Using thick sections obtained from fixed and embedded cultures of colonial P antarctica, we calculate tomographic reconstructions of individual chloroplasts under light-limiting and saturating conditions for net photosynthesis. Our goal is to gain an understanding of the continuity of thylakoid membranes and understand the spatial relationship between the pyrenoid, the starch containing organelle, and thylakoid membranes. We found that Phaeocystis showed considerable morphological and physiological flexibility in response to environmental light levels. We found that the thylakoids generally run parallel to the chloroplast membrane with many junctures and bifurcations, many of which are in contact with the chloroplast membrane itself. The considerable flexibility in the. thylakoid membranes allows for the accommodation of the pyrenoid structure. The arrangement of the thylakoids within these structures resemble those found in new structures of mitochondria cristae. We present a new structural model for algal chloroplasts which greatly revises current concepts of thylakoid membrane structure in relation to photoacclimation.

Moisan, Tiffany A.↗

Annual cycle, quasi-biennial oscillation, and Southern Oscillation in global precipitation

Using 80 years (1901-1980) of global station rainfall data, the spatial and temporal variations in global precipitation associated with the annual cycle (AC), the tropospheric quasi-biennial oscillation (QBO), and southern oscillation (SO, defined as the background 3- to 7-year fluctuation in which the El Nino events are embedded) were investigated. It was found that the dominant global precipitation pattern fluctuates irregularly, with 2- to 5-year periods associated with the SO. The temporal variation shows, in addition to the SO time scale, a distinct QBO signal. The best correlation occurred during the El Nino SO, with an apparent phase locking between the QBO and SO. The role of the possible influence of the AC and other factors involved in the QBO interaction is discussed.

Lau, K.-M.↗

Relativistic particle acceleration in plerions

We discuss recent research on the structure and particle acceleration properties of relativistic shock waves in which the magnetic field is transverse to the flow direction in the upstream medium, and whose composition is either pure electrons and positrons or primarily electrons and positrons with an admixture of heavy ions. Particle-in-cell simulation techniques as well as analytic theory have been used to show that such shocks in pure pair plasmas are fully thermalized -- the downstream particle spectra are relativistic Maxwellians at the temperature expected from the jump conditions. On the other hand, shocks containing heavy ions which are a minority constituent by number but which carry most of the energy density in the upstream medium do put approximately 20% of the flow energy into a nonthermal population of pairs downstream, whose distribution in energy space is N(E) varies as E(exp -2), where N(E)dE is the number of particles with energy between E and E+dE. The mechanism of thermalization and particle acceleration is found to be synchrotron maser activity in the shock front, stimulated by the quasi-coherent gyration of the whole particle population as the plasma flowing into the shock reflects from the magnetic field in the shock front. The synchrotron maser modes radiated by the heavy ions are absorbed by the pairs at their (relativistic) cyclotron frequencies, allowing the maximum energy achievable by the pairs to be gamma(sub +/-)m(sub +/-)c squared = m(sub i)c squared gamma(sub 1)/Z(sub i), where gamma(sub 1) is the Lorentz factor of the upstream flow and Z(sub i) is the atomic number of the ions. The shock's spatial structure is shown to contain a series of 'overshoots' in the magnetic field, regions where the gyrating heavy ions compress the magnetic field to levels in excess of the eventual downstream value. This shock model is applied to an interpretation of the structure of the inner regions of the Crab Nebula, in particular to the 'wisps,' surface brightness enhancements near the pulsar. We argue that these surface brightness enhancements are the regions of magnetic overshoot, which appear brighter because the small Larmor radius pairs are compressed and radiate more efficiently in the regions of more intense magnetic field. This interpretation suggests that the structure of the shock terminating the pulsar's wind in the Crab Nebula is spatially resolved, and allows one to measure gamma(sub 1) and a number of other properties of the pulsar's wind. We also discuss applications of the shock theory to the termination shocks of the winds from rotation-powered pulsars embedded in compact binaries. We show that this model adequately accounts for (and indeed predicted) the recently discovered X-ray flux from PSR 1957+20, and we discuss several other applications to other examples of these systems.

Arons, Jonathan↗

High Sensitivity Stress Sensor Based on Hybrid Materials

A sensing device is used to detect the spatial distributions of stresses applied by physical contact with the surface of the sensor or induced by pressure, temperature gradients, and surface absorption. The sensor comprises a hybrid active layer that includes luminophores doped in a polymeric or organic host, altogether embedded in a matrix. Under an electrical bias, the sensor simultaneously converts stresses into electrical and optical signals. Among many applications, the device may be used for tactile sensing and biometric imaging.

Cao, Xian-An↗

Generation of dynamo magnetic fields in protoplanetary and other astrophysical accretion disks

A computational method for treating the generation of dynamo magnetic fields in astrophysical disks is presented. The numerical difficulty of handling the boundary condition at infinity in the cylindrical disk geometry is overcome by embedding the disk in a spherical computational space and matching the solutions to analytically tractable spherical functions in the surrounding space. The lowest lying dynamo normal modes for a 'thick' astrophysical disk are calculated. The generated modes found are all oscillatory and spatially localized. Tha potential implications of the results for the properties of dynamo magnetic fields in real astrophysical disks are discussed.

Stepinski, T. F.↗

The high-latitude cloud MBM 7. I. H I and CO observations

The high-latitude cloud (HLC) MBM 7 has been observed in the 21 cm H I line and the 12CO(1-0) and 13CO(1-0) lines with similar spatial resolutions. The data reveal a total mass approximately 30 M solar for MBM 7 and a complex morphology. The cloud consists of a cold dense core of 5 M solar surrounded by atomic and molecular gas with about 25 M solar, which is embedded in hotter and more diffuse H I gas. We derive a total column density N(H I + 2H2) of 1 x 10(21) cm-2 toward the center and 1 x 10(20) cm-3 toward the envelope of MBM 7. The CO line indicates the existence of dense cores [n(H2) > or = 2000 cm-3] of size (FWHM) approximately 0.5 pc. The morphology suggests shock compression from the southwest direction, which can form molecular cores along the direction perpendicular to the H I distribution. The H I cloud extends to the northeast, and the velocity gradient appears to be about 2.8 km s-1 pc-1 in this direction, which indicates a systematic outward motion which will disrupt the cloud in approximately 10(6) yr. The observed large line widths of approximately 2 km s-1 for CO suggest that turbulent motions exist in the cloud, and hydrodynamical turbulence may dominate the line broadening. Considering the energy and pressure of MBM 7, the dense cores appear not to be bound by gravity, and the whole cloud including the dense cores seem to be expanding. The distance to HLCs suggest that they belong to the galactic plane, since the scale height of the cloud is < or approximately equal to 100 pc. Compared to the more familiar dense dark clouds, HLCs may differ only in their small mass and low density, with their proximity reducing the filling factor and enhancing the contrast of the core and envelope structure.

Non-NASA Center↗

Spectral feature classification and spatial pattern recognition

This paper introduces a spatial pattern recognition processing concept involving the use of spectral feature classification technology and coherent optical correlation. The concept defines a hybrid image processing system incorporating both digital and optical technology. The hybrid instrument provides simplified pseudopattern images as functions of pixel classification from information embedded within a real-scene image. These pseudoimages become simplified inputs to an optical correlator for use in a subsequent pattern identification decision useful in executing landmark pointing, tracking, or navigating functions. Real-time classification is proposed as a research tool for exploring ways to enhance input signal-to-noise ratio as an aid in improving optical correlation. The approach can be explored with developing technology, including a current NASA Langley Research Center technology plan that involves a series of related Shuttle-borne experiments. A first-planned experiment, Feature Identification and Location Experiment (FILE), is undergoing final ground testing, and is scheduled for flight on the NASA Shuttle (STS2/flight OSTA-1) in 1980. FILE will evaluate a technique for autonomously classifying earth features into the four categories: bare land; water; vegetation; and clouds, snow, or ice.

Sivertson, W. E., Jr.↗

High Sensitivity, High Angular Resolution Far-infrared Photometry from the KAO

Most of the luminosity of embedded sources is reemitted in the far-infrared continuum. Measurements in the far-infrared are essential to understand the energetics of the interstellar medium, and of star formation regions in particular. Measurements from the KAO, are made in diffraction limited beams that sample a spatial scale considerably smaller than that given by IRAS. The KAO instrument technology has matured to the point that the single scan limiting flux of IRAS at 100 micro can be reached in a diffraction limited beam in a single typical KAO observing leg. The far-infrared photometer system and selections of recent observations are presented.

Lester, D.↗