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Method Development for In-situ Detection of Latent Herpesvirus DNA from Saliva using Nanopore Sequencing

Research toward latent herpesvirus reactivation has been intensively addressed through Space Shuttle and International Space Station (ISS) investigations. This work has provided the understanding that persistent reactivation of herpesviruses from asymptomatic crew can be detected through viral shedding in saliva, urine, and blood. Occasionally, viral reactivation from the latency stage can pose a threat to crew health (clinical manifestation) before, during, and after flight missions. Furthermore, previous work detailing correlations with immunity indicate that monitoring viral reactivation could be implemented to assess potential immune dysfunction. While in-flight monitoring is desirable, there is no well-established procedure or method for real-time evaluations, and research to date has relied on postflight, ground-based analysis. The development of portable molecular technologies like the miniPCR™ (miniPCR Bio) thermal cycler and the MinION™ sequencer (Oxford Nanopore Technologies) have confirmed that real-time monitoring is possible in extreme and low resource environments. These devices, combined with simple sample preparation methods, have been used to demonstrate bacterial identification onboard the ISS, as well as rapid viral detection in remote locations on Earth. The work here builds upon previous molecular advancements onboard the ISS toward the development and validation of a spaceflight-compatible method for viral detection from crew samples. Several herpesviruses can be detected in saliva, which provides a non-invasive means to collect samples for monitoring. While the basis for this method lies in previous spaceflight investigations, key points for method optimization include DNA extraction from saliva, viral primer selection, and bioinformatic processes for data analysis. To increase viral yield, numerous DNA extraction methods have been evaluated and will be discussed in detail. For initial development and testing, varicella-zoster virus (VZV) is being targeted though open reading frame 51 and 63 (ORF51, ORF63), as the replication origin-binding protein is highly expressed during latency. Optimization of the thermal cycling parameters has resulted in the ability to test the entire process. The full method has been tested with both viral VZV DNA standards and saliva spiked with varying concentrations of VZV. Viral sequence data were mapped to the reference sequence using minimap2. Prior to mapping, DNA sequencing reads were filtered for length and quality, barcodes were removed, and alignment identity calculated. Following further assessments, statistics were compared across multiple sequencing experiments and are being used to determine the success of the protocols. Forward work will include the incorporation of herpes simplex virus 1 (HSV-1) and Epstein-Barr virus (EBV) primers as well as the validation of results to the terrestrial qPCR standard assay. Upon full validation of the developed method, saliva will be collected from 20 healthy subjects and spiked with viral DNA. These samples will be split and assayed with the MinION and standard qPCR assay.

Hang N Nguyen↗

Detecting differential ground displacements of civil structures in fast-subsiding metropolises with interferometric SAR and band-pass filtering

Ground displacements due to changes in soil conditions represent a threat to the stability of civil structures in many urban areas, worldwide. In fast-subsiding areas, regional subsidence (wavelength ~ 1,000’s m) can be dominantly high and, consequently, mask other signals at local scales (wavelength ~ 10–100’s m). Still, engineering and construction applications require a comprehensive knowledge of local-scale signals, which can threaten the stability of buildings and infrastructure. Here we present a new technique based on band-pass filters for uncovering local-scale signals hidden by regional subsidence as detected by interferometric SAR measurements. We apply our technique to a velocity field calculated from 21 high-resolution COSMO-SkyMed scenes acquired over Mexico City and obtain components of long (> 478 m), intermediate (42–478 m) and short (< 42 m) spatial wavelengths. Our results reveal that long-wavelength velocities exceed − 400 mm/year, whereas intermediate- and short-wavelength velocities are in the order of ± 15 mm/year. We show that intermediate-wavelength velocities are useful for retrieving signals such as uplift along elevated viaducts of Metro lines 4 and B, as well as differential displacements in Pantitlán station’s pedestrian overpass system and across sharp geotechnical boundaries in the piedmont of Sierra de Santa Catarina—where surface faulting occurs.

Darío Solano-Rojas↗

Testing of Advanced Capabilities to Enable In-time Safety Management and Assurance for Future Flight Operations

In order to refine an initial Concept of Operations, explore Concepts of Use, and expose/validate requirements for future In-Time Aviation Safety Management Systems (IASMS), testing architectures were created, along with a set of capabilities and underlying information exchange protocols. These systems were conceived and developed based on hazards associated with two envisioned urban area flight domains: (1) highly autonomous small uncrewed aerial systems (sUAS) operating at low altitudes, and (2) highly autonomous air taxis. The initial scope of this development is described in [1]; this report provides an update, focusing on the subsequent developments and test activities. As stated in [1], it is important to note that there are many capabilities already in use by the industry (or soon to be in use) that will play critical roles in future IASMS designs. Those reported here were developed to address a gap in the current state-of-the-art regarding specific hazards/risks, and/or to allow for investigation of the interplay between and across hazard types — particularly regarding how overall safety risk can be reduced or managed effectively. Results of testing and development activities are organized by the operational phase wherein a particular capability would be employed (i.e., preflight, in-flight, and post-flight/off-line). Pre-flight: A set of capabilities were developed to help mitigate safety risk prior to flight (e.g., during flight and mission planning). Results of testing summarize (1) validation activities to raise the Technology Readiness Level (TRL) and (2) evaluation activities where the capabilities were applied to flight/mission planning procedures and used by operators/pilots. For the latter, flight plans were automatically assessed, and operators/pilots were notified of hazardous flight segments so as to enable adjustment of the flight plan and re-evaluation, and/or to better inform go/no-go decisions. Capabilities addressed hazards associated with power consumption, third-party risk, wind, navigation system performance, radiofrequency interference, and proximity to geo-spatial threats (e.g., buildings, trees, and no-fly zones). In-flight: Flight experiments tested capabilities that detect and respond to hazards encountered during flight. In the first series, safety hazards were monitored and assessed onboard, and system-generated mitigation maneuvers were recorded (but not acted upon by the vehicle). In the second series, mitigation maneuver commands directed the aircraft in response to safety hazards (i.e., auto-mitigation). The sUAS used for testing is described in full, as is the test architecture, which included commercial avionics, research avionics, and onboard software designed to detect, assess, and respond to hazards. The onboard system was designed as a run-time assurance framework, consistent with [2] and supportive of both supervisory and automated modes. The primary functions included: real-time risk assessment (RTRA), auto-pilot monitoring, constraint monitoring, and contingency select/triggering. RTRA performs integrated risk assessment considering data from several hazard-related monitors (e.g., battery, motors, navigation, communications, population density, and loss-of-control). Post-flight/off-line: Data monitored and recorded during flights can enable IASMS capabilities that execute after flights have completed (or “off-line”). These include: (1) the ability to identify anomalies and trends that may only be observable when comparing data spanning a number of similar flights; (2) the ability to update and validate pre-flight and in-flight capabilities and any underlying models to improve their performance; (3) the ability to report anomalies/off-nominals that may indicate design changes or maintenance actions are needed; and (4) the ability for humans involved in operations to report safety-relevant observations to help in understanding the flight data and/or the operational context of a flight. Progress on three such capabilities is summarized; the first investigates anomaly detection given a limited set of flight logs and applies an approach previously used for space operations. The second explores what could be identified using a larger set of flight logs, including from web-based forums where flight logs are posted by sUAS autopilot users. The third creates a new means of collecting information on UAS incidents and accidents via the Aviation Safety Reporting System (ASRS).

sUAS↗

Human Expeditions to Near-Earth Asteroids: Implications for Exploration, Resource Utilization, Science, and Planetary Defense

Over the past several years, much attention has been focused on human exploration of near-Earth asteroids (NEAs) and planetary defence. Two independent NASA studies examined the feasibility of sending piloted missions to NEAs, and in 2009, the Augustine Commission identified NEAs as high profile destinations for human exploration missions beyond the Earth-Moon system as part of the Flexible Path. More recently the current U.S. presidential administration directed NASA to include NEAs as destinations for future human exploration with the goal of sending astronauts to a NEA in the mid to late 2020s. This directive became part of the official National Space Policy of the United States of America as of June 28, 2010. With respect to planetary defence, in 2005 the U.S. Congress directed NASA to implement a survey program to detect, track, and characterize NEAs equal or greater than 140 m in diameter in order to access the threat from such objects to the Earth. The current goal of this survey is to achieve 90% completion of objects equal or greater than 140 m in diameter by 2020.

Abell, Paul↗

Detecting Hail from Space: Using a Multi-Frequency Passive-Microwave Retrieval to Analyze the Global Climatology and Diurnal Cycle of Severe Hail

Severe hail poses myriad threats to society, causing extensive damage to infrastructure and agriculture. As hail is severe, relatively infrequent, and highly localized, it is difficult to measure in-situ and if left unresolved in models and precipitation retrievals, hail can cause large errors and uncertainties. The difficulty in measuring hail in situ and the inconsistency of surface-based hail reporting drive the motivation to use spaceborne remote-sensing platforms to retrieve hail and construct climatologies in a globally uniform way. We leverage the scattering signatures of severe hail in spaceborne passive-microwave datasets paired with surface hail reports to construct a multi-frequency hail retrieval using Tropical Rainfall Measuring Mission (TRMM) microwave imager (TMI) data. Using coincident Global Precipitation Measurement (GPM) Ku-band precipitation radar, we assessed this retrieval and several others in the literature for their effectiveness and regional variability. We use this retrieval to construct global passive-microwave climatologies of severe hail using the TRMM, GPM, Advanced Microwave Scanning Radiometer for EOS (AMSR-E), and Advanced Microwave Scanning Radiometer 2 (AMSR2) sensors and extend into the pre-TRMM era to the Special Sensor Microwave Imager/Sounder (SSMI/(S)) data. We also leverage the sensors in inclined orbits to assess the diurnal variability of severe hail globally and the effect the diurnal cycle has on the detection of hail by sensors in sun-synchronous orbit. The goal is to construct a robust, multi-decade multi-satellite climatology of hail. As part of the NASA Disasters Applied Sciences program, we assess these climatologies against other satellite severe weather datasets and use these climatologies in collaboration with stakeholders and end-users to help them assess risk, and improve the prediction, preparation, and response to severe storms around the world.

Sarah D Bang↗

Detection of Antimicrobial Resistance Genes Associated With the International Space Station Environmental Surfaces

Antimicrobial resistance (AMR) is a global health issue. In an effort to minimize this threat to astronauts, who may be immunocompromised and thus at a greater risk of infection from antimicrobial resistant pathogens, a comprehensive study of the ISS “resistome’ was conducted. Using whole genome sequencing (WGS) and disc diffusion antibiotic resistance assays, 9 biosafety level 2 organisms isolated from the ISS were assessed for their antibiotic resistance. Molecular analysis of AMR genes from 24 surface samples collected from the ISS during 3 different sampling events over a span of a year were analyzed with Ion AmpliSeq™ and metagenomics. Disc diffusion assays showed that Enterobacter bugandensis strains were resistant to all 9 antibiotics tested and Staphylococcus haemolyticus being resistant to none. Ion AmpliSeq™ revealed that 123 AMR genes were found, with those responsible for beta-lactam and trimethoprim resistance being the most abundant and widespread. Using a variety of methods, the genes involved in antimicrobial resistance have been examined for the first time from the ISS. This information could lead to mitigation strategies to maintain astronaut health during long duration space missions when return to Earth for treatment is not possible.

Antimicrobial resistance↗

Preliminary analysis of the JAPE ground vehicle test data with an artificial neural network classifier

Remotely sensing and classifying military vehicles in a battlefield environment have been the source of much research over the past 20 years. The ability to know where threat vehicles are located is an obvious advantage to military personnel. In the past active methods of ground vehicle detection such as radar have been used, but with the advancement of technology to locate these active sensors, passive sensors are preferred. Passive sensors detect acoustic emissions, seismic movement, electromagnetic radiation, etc., produced by the target and use this information to describe it. Deriving the mathematical models to classify vehicles in this manner has been, and is, quite complex and not always reliable. However, with the resurgence of artificial neural network (ANN) research in the past few years, developing models for this work may be a thing of the past. Preliminary results from an ANN analysis to the tank signatures recorded at the Joint Acoustic Propagation Experiment (JAPE) at the US Army White Sands Missile Range, NM, in July 1991, are presented.

Larsen, Nathan F.↗

Creating a Deformation Time Series Utilizing InSAR to Assess Hazards and Detect Volcanic Unrest in Clear Lake Volcanic Field, California

According to the U.S Geological Survey, the Clear Lake Volcanic Field (CLVF) in northern California is at a high threat potential for volcanic hazards. Eruptions leading to increased seismic activity could result in silicic domes, cinder cones, and flows that would be dangerous to the residential areas near the volcanic field. Remotely sensed Earth observations can reveal volcanic processes in the subsurface, which are essential to the timely monitoring of potential volcanic activity. In particular, Sentinel-1 C-band Synthetic Aperture Radar (C-SAR) and Digital Elevation Model (DEM) data capture relative surface deformation at unprecedented high spatial and temporal resolutions. Leveraging C-SAR and DEMs, we conducted interferometric analysis from January 2016 to December 2023. Our results demonstrate 1) the mean surface displacement velocity of the Clear Lake Volcanic field is measured to undergo 5 to 10-centimeter scale deformation and shows a strong correlation with the surrounding faults, 2) apparent seasonal differences in rates of surface change, and 3) seismic activity associated with the geyser geothermal field has a strong correlation with cumulative surface displacement, with active fault zones having 2 to 5 cm of additional displacement. Results indicate that deformation is linked to deep pressure sources causing stresses on the surficial environment that should be considered in hazard mitigation. This study provides a baseline of historic deformation, aiding hazard analysts in communication efforts and streamlining decision-making for potential risks to region residents.

Ivan Tochimani-Hernandez↗

Measuring ground movement in geothermal areas of Imperial Valley, California

Significant ground movement may accompany the extraction of large quantities of fluids from the subsurface. In Imperial Valley, California, one of the potential hazards of geothermal development is the threat of both subsidence and horizontal movement of the land surface. Regional and local survey nets are being monitored to detect and measure possible ground movement caused by future geothermal developments. Precise measurement of surface and subsurface changes will be required to differentiate man-induced changes from natural processes in this tectonically active region.

Lofgren, B. E.↗

Mars Sample Handling Protocol Workshop Series: Workshop 2

Numerous NASA reports and studies have identified Planetary Protection (PP) as an important part of any Mars sample return mission. The mission architecture, hardware, on-board experiments, and related activities must be designed in ways that prevent both forward- and back-contamination and also ensure maximal return of scientific information. A key element of any PP effort for sample return missions is the development of guidelines for containment and analysis of returned sample(s). As part of that effort, NASA and the Space Studies Board (SSB) of the National Research Council (NRC) have each assembled experts from a wide range of scientific fields to identify and discuss issues pertinent to sample return. In 1997, the SSB released its report on recommendations for handling and testing of returned Mars samples. In particular, the NRC recommended that: a) samples returned from Mars by spacecraft should be contained and treated as potentially hazardous until proven otherwise, and b) rigorous physical, chemical, and biological analyses [should] confirm that there is no indication of the presence of any exogenous biological entity. Also in 1997, a Mars Sample Quarantine Protocol workshop was convened at NASA Ames Research Center to deal with three specific aspects of the initial handling of a returned Mars sample: 1) biocontainment, to prevent 'uncontrolled release' of sample material into the terrestrial environment; 2) life detection, to examine the sample for evidence of organisms; and 3) biohazard testing, to determine if the sample poses any threat to terrestrial life forms and the Earth's biosphere. In 1999, a study by NASA's Mars Sample Handling and Requirements Panel (MSHARP) addressed three other specific areas in anticipation of returning samples from Mars: 1) sample collection and transport back to Earth; 2) certification of the samples as non-hazardous; and 3) sample receiving, curation, and distribution. To further refine the requirements for sample hazard testing and the criteria for subsequent release of sample materials from quarantine, the NASA Planetary Protection Officer convened an additional series of workshops beginning in March 2000. The overall objective of these workshops was to develop comprehensive protocols to assess whether the returned materials contain any biological hazards, and to safeguard the purity of the samples from possible terrestrial contamination. This document is the report of the second Workshop in the Series. The information herein will ultimately be integrated into a final document reporting the proceedings of the entire Workshop Series along with additional information and recommendations.

Rummel, John D.↗

An Overview of Ground-Based Radar and Optical Measurements Utilized By the Nasa Orbital Debris Program Office

For over 30 years, the NASA Orbital Debris Program Office (ODPO) has led the characterization of orbital debris (OD) too small to be tracked by the U.S. Space Surveillance Network (SSN), yet which may pose the greatest threat to human spaceflight and robotic missions. Measurements from specialized sensors, including ground-based radars and telescopes capable of detecting smaller objects, provide the foundation for developing statistical models to describe the current state and future evolution of the OD environment from low Earth orbit (LEO) to geosynchronous Earth orbit (GEO). Since 1990, the ODPO has partnered with the U.S. Department of Defense and the Massachusetts Institute of Technology Lincoln Laboratory (MIT/LL) to collect data using the Haystack Ultrawideband Satellite Imaging Radar (HUSIR) – formerly Haystack – to characterize OD in LEO with a sensitivity of approximately 5 mm at 1000 km altitude. In addition, since 1993, the Goldstone Orbital Debris Radar, operated by NASA’s Jet Propulsion Laboratory, has provided data on OD as small as approximately 2-3 mm for altitudes below 1000 km, some of the most sensitive ground-based measurements achievable at these altitudes. Recently, collaborations with the 18 th Space Control Squadron of the U.S. Space Force have also provided the ODPO with special datasets from the Space Fence to extend coverage below the historical SSN limit of 10 cm and to characterize individual breakup events in LEO. For GEO altitudes, the Eugene Stansbery Meter Class Autonomous Telescope (ES-MCAT), a joint NASA-Air Force Research Laboratory project that reached full operational capability in 2021, collects data on debris smaller than 1 m and provides coverage of debris in historically under-sampled high-altitude orbital regimes. This paper summarizes the radar and optical sensors utilized by the ODPO, their unique capabilities, and recent datasets and applications for statistical sampling of the dynamic OD environment.

Alyssa Manis↗

An Overview of Ground-Based Radar and Optical Measurements Utilized By the Nasa Orbital Debris Program Office

For over 30 years, the NASA Orbital Debris Program Office (ODPO) has led the characterization of orbital debris (OD) too small to be tracked by the U.S. Space Surveillance Network (SSN), yet which may pose the greatest threat to human spaceflight and robotic missions. Measurements from specialized sensors, including ground-based radars and telescopes capable of detecting smaller objects, provide the foundation for developing statistical models to describe the current state and future evolution of the OD environment from low Earth orbit (LEO) to geosynchronous Earth orbit (GEO). Since 1990, the ODPO has partnered with the U.S. Department of Defense and the Massachusetts Institute of Technology Lincoln Laboratory (MIT/LL) to collect data using the Haystack Ultrawideband Satellite Imaging Radar (HUSIR) – formerly Haystack – to characterize OD in LEO with a sensitivity of approximately 5 mm at 1000 km altitude. In addition, since 1993, the Goldstone Orbital Debris Radar, operated by NASA’s Jet Propulsion Laboratory, has provided data on OD as small as approximately 2-3 mm for altitudes below 1000 km, some of the most sensitive ground-based measurements achievable at these altitudes. Recently, collaborations with the 18 th Space Control Squadron of the U.S. Space Force have also provided the ODPO with special datasets from the Space Fence to extend coverage below the historical SSN limit of 10 cm and to characterize individual breakup events in LEO. For GEO altitudes, the Eugene Stansbery Meter Class Autonomous Telescope (ES-MCAT), a joint NASA-Air Force Research Laboratory project that reached full operational capability in 2021, collects data on debris smaller than 1 m and provides coverage of debris in historically under-sampled high-altitude orbital regimes. This paper summarizes the radar and optical sensors utilized by the ODPO, their unique capabilities, and recent datasets and applications for statistical sampling of the dynamic OD environment.

Alyssa Manis↗

SC-228 Inclusion of DAA Warning Alert for TCAS Interoperability

This white paper summarizes NASA research results that have informed Special Committee 228 (SC-228) discussions and decisions regarding the inclusion of a warning-level alert within the detect and avoid (DAA) alerting structure for unmanned aircraft systems (UAS). For UAS, the removal of the pilot from onboard the aircraft has eliminated the ability of the ground-based pilot in command (PIC) to use out-the-window visual information to make judgments about a potential threat of a loss of well clear with another aircraft. As a result, the DAA traffic display will be the primary source of information that the PIC can use to execute the three primary well clear functions: 1) detect a potential loss of well clear, 2) determine a resolution maneuver, and 3) upload that maneuver to the aircraft via the ground control station (GCS). In addition, pilots are required to coordinate with air traffic control (ATC) prior to maneuvering off of their approved flight plan. In determining an appropriate resolution maneuver to avoid a loss of well clear, the PIC must decide both when and how to maneuver, and both the timeliness and the accuracy (i.e., correctness) of the maneuver are critical to reducing the likelihood and/or severity of a loss of well clear. Alerting information is one of three critical components of the DAA display, along with traffic information elements (e.g., relative heading, speed and altitude) and maneuver guidance. Alerting information and maneuver guidance, in particular, have been found to have a significant impact, both statistically and practically, on pilots' ability to avoid and minimize the severity of losses of well clear While all three display components are key to pilots performing the traffic avoidance task of remaining well clear, in general, alerting information provides crucial information about when a resolution maneuver is required while maneuver guidance assists the pilot in determining how best to maneuver. A fundamental task of the DAA alerting system is to provide critical timing information to the pilot about the potential for a loss of well clear with another aircraft. This is done by employing both temporal and spatial thresholds that indicate to the pilot the likelihood and imminence of a loss of well clear. The design of the DAA alerting thresholds is a balancing act between eliciting the desired pilot response in real loss of well clear threat events and reducing excessive, unnecessary, and/or uncoordinated UAS maneuvering within the air traffic environment; larger thresholds, both spatially and temporally, may increase the likelihood of a pilot avoiding a loss of well clear, but it can also increase the frequency of maneuvering - especially in cases where a maneuver is not actually needed to maintain well clear. A series of human in the loop (HITL) simulations have been conducted as part of NASA's Unmanned Aircraft Systems (UAS) Integration in the National Airspace System (NAS) project. The purpose of these HITLs has been to provide empirical results in order to inform development of the minimum human-machine interface requirements for the DAA system. This white paper will present those results which provide evidence of a human performance benefit (in terms of response times and ability to remain well clear of other aircraft) of the DAA warning alert both with and without a collision avoidance system on board the aircraft.

TCAS interoperability↗

Mars Sample Quarantine Protocol Workshop

The Mars Sample Quarantine Protocol (QP) Workshop was convened to deal with three specific aspects of the initial handling of a returned Mars sample: 1) biocontainment, to prevent uncontrolled release of sample material into the terrestrial environment; 2) life detection, to examine the sample for evidence of live organisms; and 3) biohazard testing, to determine if the sample poses any threat to terrestrial life forms and the Earth's biosphere. During the first part of the Workshop, several tutorials were presented on topics related to the workshop in order to give all participants a common basis in the technical areas necessary to achieve the objectives of the Workshop.

DeVincenzi, Donald L.↗

Early Oscillation Detection for DC/DC Converter Fault Diagnosis

The electrical power system of a spacecraft plays a very critical role for space mission success. Such a modern power system may contain numerous hybrid DC/DC converters both inside the power system electronics (PSE) units and onboard most of the flight electronics modules. One of the faulty conditions for DC/DC converter that poses serious threats to mission safety is the random occurrence of oscillation related to inherent instability characteristics of the DC/DC converters and design deficiency of the power systems. To ensure the highest reliability of the power system, oscillations in any form shall be promptly detected during part level testing, system integration tests, flight health monitoring, and on-board fault diagnosis. The popular gain/phase margin analysis method is capable of predicting stability levels of DC/DC converters, but it is limited only to verification of designs and to part-level testing on some of the models. This method has to inject noise signals into the control loop circuitry as required, thus, interrupts the DC/DC converter's normal operation and increases risks of degrading and damaging the flight unit. A novel technique to detect oscillations at early stage for flight hybrid DC/DC converters was developed.

Wang, Bright L.↗

Diagnosing Aircraft Icing Potential from Satellite Cloud Retrievals

The threat for aircraft icing in clouds is a significant hazard that routinely impacts aviation operations. Accurate diagnoses and forecasts of aircraft icing conditions requires identifying the location and vertical distribution of clouds with super-cooled liquid water (SLW) droplets, as well as the characteristics of the droplet size distribution. Traditional forecasting methods rely on guidance from numerical models and conventional observations, neither of which currently resolve cloud properties adequately on the optimal scales needed for aviation. Satellite imagers provide measurements over large areas with high spatial resolution that can be interpreted to identify the locations and characteristics of clouds, including features associated with adverse weather and storms. This paper describes new techniques for interpreting cloud products derived from satellite data to infer the flight icing threat to aircraft. For unobscured low clouds, the icing threat is determined using empirical relationships developed from correlations between satellite imager retrievals of liquid water path and droplet size with icing conditions reported by pilots (PIREPS). For deep ice over water cloud systems, ice and liquid water content (IWC and LWC) profiles are derived by using the imager cloud properties to constrain climatological information on cloud vertical structure and water phase obtained apriori from radar and lidar observations, and from cloud model analyses. Retrievals of the SLW content embedded within overlapping clouds are mapped to the icing threat using guidance from an airfoil modeling study. Compared to PIREPS and ground-based icing remote sensing datasets, the satellite icing detection and intensity accuracies are approximately 90% and 70%, respectively, and found to be similar for both low level and deep ice over water cloud systems. The satellite-derived icing boundaries capture the reported altitudes over 90% of the time. Satellite analyses corresponding to the time and location of several recent aviation accidents and with icing PIREPS are also presented that reveal skill in identifying severe icing conditions. These results demonstrate the utility of satellite cloud retrievals for quantitatively diagnosing the potential for icing conditions on temporal and spatial scales that should be useful to the aviation community. Plans are being developed to deliver these new satellite products to the GOES-R Proving Ground in the near future so that they can be evaluated in operational applications.

Smith, William L., Jr.↗

Laser Ablation Surface Treatment of Structural Aerospace Composites and Metal Alloys for Adhesive Bonding

NASA Langley Research Center (LaRC) has been involved over the past decade in the development of laser ablation as a method to surface treat aerospace structural fiber reinforced composites (CFRPs) and metal alloys. [1- 18] Laser surface treatment has been shown to be a rapid, high precision, and reproducible method for surface treatment of these materials, and is amenable to automation. The laser ablation process removes surface contamination such as silicones, creates surface roughness, and chemically activates a surface for bonding. The degree of ablation is controllable by adjusting laser parameters. In the case of CFRPs, ablation can be performed without damage to any underlying carbon fibers. In the case of metal alloys, ablation is possible without surface embrittlement, heat damage or creation of undesirable grain structures or morphologies. The optimum laser treatment conditions are substrate dependent and generally are influenced by laser wavelength and pulse duration (typically fixed for a laser system), and laser power. In the case of metal alloys such as Ti-6Al-4V, the laser ablation process eliminates the need for several steps of the conventional processes, including hydrofluoric (HF) acid and caustic treatment. These steps are often conducted in dip tanks and require costly facilities and environmentally unfriendly liquids. In addition, the laser can create nano-porosity on the Ti-6Al-4V alloy surface allowing for sol-gel penetration and thereby creating a gradient interface with improved temperature and mechanical performance. NASA LaRC has also been involved with the development of in-line characterization methods compatible with the laser treatment process to rapidly determine the presence of key contaminates such as silicones that, even at very low concentrations, are a threat to adhesive bonding. A spectroscopic technique called micro-laser induced breakdown spectroscopy (u-LIBS) has been developed to rapidly detect silicones at concentrations below those known to cause bonding problems. This technique requires no sample preparation, vacuum or inert atmosphere, has no practical size limitations, and provides nearly instantaneous feedback to the operator. This presentation will give an overview and update of these developments with specific examples highlighted.

Connell, John W.↗

Martian Neutron Energy Spectrometer (MANES)

High energy charged particles of extragalactic, galactic, and solar origin collide with spacecraft structures and planetary atmospheres. These primaries create a number of secondary particles inside the structures or on the surfaces of planets to produce a significant radiation environment. This radiation is a threat to long term inhabitants and travelers for interplanetary missions and produces an increased risk of carcinogenesis, central nervous system (CNS) and DNA damage. Charged particles are readily detected; but, neutrons, being electrically neutral, are much more difficult to monitor. These secondary neutrons are reported to contribute 30-60% of the dose equivalent in the Shuttle and MIR station. The Martian atmosphere has an areal density of 37 g/sq cm primarily of carbon dioxide molecules. This shallow atmosphere presents fewer mean free paths to the bombarding cosmic rays and solar particles. The secondary neutrons present at the surface of Mars will have undergone fewer generations of collisions and have higher energies than at sea level on Earth. Albedo neutrons produced by collisions with the Martian surface material will also contribute to the radiation environment. The increased threat of radiation damage to humans on Mars occurs when neutrons of higher mean energy traverse the thin, dry Martian atmosphere and encounter water in the astronaut's body. Water, being hydrogeneous, efficiently moderates the high energy neutrons thereby slowing them as they penetrate deeply into the body. Consequently, greater radiation doses can be deposited in or near critical organs such as the liver or spleen than is the case on Earth. A second significant threat is the possibility of a high energy heavy ion or neutron causing a DNA double strand break in a single strike.

Maurer, R. H.↗