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At least 91 records · Page 5

Reflectance differences between Target and Torch rape cultivars

Spectroradiometric reflectance measurements were made on Target and Torch plants (four and five leaves, respectively) that were growing in 0.09 m2 soil-containing flats. Torch's spectrophotometric single leaf reflectance was consistently lower than Target's at the 650-nm chlorophyll absorption band because Torch's chlorophyll concentration was larger than Target's, which caused more red light absorption. Spectroradiometric measurements indicate that: wet soil strongly absorbs visible light (500 to 700 nm) so that Target's soil-containing flat with 60% plant cover has less reflectance than Torch's soil-containing flat with 75% plant cover; Torch (most foiliage) has higher near-infrared (750 to 1,350 nm) reflectance than Target (least foliage); and the 2,200-nm wavelength is a candidate band to distinguish Target from Torch. The difference in chlorophyll concentrations between Target and Torch, compared with leaf structural differences, is apparently the most important factor that would affect the infrared color film's tonal response to vegetation in the photographic sensitive region (500 to 900 nm).

Gausman, H. W.↗

Mesoscale Variations of Biogeochemical Properties in the Sargasso Sea

A mesoscale resolution biogeochemical survey was carried out in the vicinity of the U.S. Joint Global Ocean Flux Study Bermuda Atlantic Time-series Study (BATS) site during the summer of 1996. Real-time nowcasting and forecasting of the flow field facilitated adaptive sampling of several eddy features in the area. Variations in upper ocean nutrient and pigment distributions were largely controlled by vertical isopycnal displacements associated with the mesoscale field. Shoaling density surfaces tended to introduce cold, nutrient-rich water into the euphotic zone, while deepening isopycnals displaced nutrient-depleted water downward. Chlorophyll concentration was generally enhanced in the former case and reduced in the latter. Eddy-induced upwelling at the base of the euphotic zone was affected by features of two different types captured in this survey-, (1) a typical mid-ocean cyclone in which doming of the main thermocline raised the near-surface stratification upward and (2) a mode water eddy composed of a thick lens of 18C water, which pushed up the seasonal thermocline and depressed the main thermocline. Model hindcasts using all available data provide a four-dimensional context in which to interpret temporal trends at the BATS site and two other locations during the 2 weeks subsequent to the survey. Observed changes in near-surface structure at the BATS site included shoaling isopycnals, increased nutrient availability at the base of the euphotic zone, and enhanced chlorophyll concentration within the cuphotic zone. These trends are explicable in terms of a newly formed cyclone that impinged upon the site during this time period. These observations reveal that eddy upwelling has a demonstrable impact on the way in which the nitrate-density relationship changes with depth from the aphotic zone into the euphotic zone. A similar transition is present in the BATS record, suggesting that eddy-driven upwelling events are present in the time series of upper ocean biogeochemical properties. The variability in main thermocline temperature and nitrate in this synoptic spatial survey spans the range observed in these quantities in the 10-year time series available at BATS to date (1988-1998).

McGillicuddy, D. J.↗

Mesoscale Variations of Biogeochemical Properties in the Sargasso Sea

A mesoscale resolution biogeochemical survey was carried out in the vicinity of the US Joint Global Ocean Flux Study Bermuda Atlantic Time-Series Study (BATS) site during the summer of 1996. Real-time nowcasting and forecasting of the flow field facilitated adaptive sampling of several eddy features in the area. Variations in upper ocean nutrient and pigment distributions were largely controlled by vertical isopycnal displacements associated with the mesoscale field. Shoaling density surfaces tended to introduce cold, nutrient-rich water into the euphotic zone, while deepening isopycnals displaced nutrient-depleted water downward. Chlorophyll concentration was generally enhanced in the former case and reduced in the latter. Eddy-induced upwelling at the base of the euphotic zone was affected by features of two different types captured in this survey: (1) a typical mid-ocean cyclone in which doming of the main thermocline raised the near-surface stratification upward; and (2) a mode water eddy composed of a thick lens of 18 C water, which pushed up the seasonal thermocline and depressed the main thermocline. Model hindcasts using all available data provide a four-dimensional context in which to interpret temporal trends at the BATS site and two other locations during the two weeks subsequent to the survey. Observed changes in near-surface structure at the BATS site included shoaling iscpycnals, increased nutrient availability at the base of the euphotic zone, and enhanced chlorophyll concentration within the euphotic zone. These trends are explicable in terms of a newly formed cyclone that impinged upon the site during this time period. These observations reveal that eddy upwelling has a demonstrable impact on the way in which the nitrate-density relationship changes with depth from the aphotic zone into the euphotic zone. A similar transition is present in the BATS record, suggesting that eddy-driven upwelling events are present in the time series of upper ocean biogeochemical properties. The variability in main thermocline temperature and nitrate in this synoptic spatial survey spans the range observed in these quantities in the 10-year time series available at BATS to date (1988-1998).

McGillicuddy Dennis J., Jr.↗

Phytoplankton Assemblage Patterns in the Southern Mid-Atlantic Bight

As part of the Wallops Coastal Oceans Observing Laboratory (Wa-COOL) Project, we sampled a time-series transect in the southern Mid-Atlantic Bight (MAB) biweekly. Our 2-year time-series data included physical parameters, nutrient concentrations, and chlorophyll a concentrations. A detailed phytoplankton assemblage structure was examined in the second year. During the 2-year study, chlorophyll a concentration (and ocean color satellite imagery) indicated that phytoplankton blooms occurred in January/February during mixing conditions and in early autumn under stratified conditions. The chlorophyll a concentrations ranged from 0.25 microgram 1(exp -1) to 15.49 microgram 1(exp -1) during the 2-year period. We were able to discriminate approximately 116 different species under phase contrast microscopy. Dominant phytoplankton included Skeletonema costatum, Rhizosolenia spp., and Pseudo-nitzschia pungens. In an attempt to determine phytoplankton species competition/succession within the assemblage, we calculated a Shannon Weaver diversity index for our diatom microscopy data. Diatom diversity was greatest during the winter and minimal during the spring. Diatom diversity was also greater at nearshore stations than at offshore stations. Individual genera appeared patchy, with surface and subsurface patches appearing abruptly and persisting for only 1-2 months at a time. The distribution of individual species differed significantly from bulk variables of the assemblage (chlorophyll a ) and total phytoplankton assemblage (cells), which indicates that phytoplankton species may be limited in growth in ways that differ from those of the total assemblage. Our study demonstrated a highly diverse phytoplankton assemblage throughout the year, with opportunistic species dominating during spring and fall in response to seasonal changes in temperature and nutrients in the southern MAB.

Mid-Atlantic↗

Response of Two Plant Species to Two Ultraviolet-B Radiation Regimes

The depleted stratospheric ozone layer has been directly linked to increased levels of ultraviolet radiation at the earth's surface. It is important to understand what effect this will have on plants. We tested the hypothesis that in response to increased UV-B radiation (280-320 man), soybean (Glycine max Merrill) and alfalfa (Mercado Saliva L.) would produce higher concentrations of flavonoids than plants screened from UV-B. Soybean and alfalfa plants were grown successively in a growth chamber that provided UV-B radiation intensities 45% above summer field levels. A wooden frame was used to suspend mylar-D film over one group of plants and mono-acetate film over another group. Mylar is opaque in the 280-316 nm range, and acetate absorbs most radiation from 280-290 nm and then reduces intensities in the 290-320 nm range by roughly 15%. Leaf chlorophyll concentration was determined with a Minolta SPAD-502 chlorophyll meter; the BRAD meter was calibrated with N,N- extractions. Flavonoids were extracted with an acidified methanol/water solution. Soybean grown under the acetate treatment showed 26% smaller internodal lengths and higher concentrations of flavonoids compared to plants grown under mylar. Significant results for alfalfa included 22% greater leaf flavonoid concentration under acetate, 14% greater leaf chlorophyll concentration under mylar, and 32% greater above-ground biomass under mylar. We found that increased UV-B radiation leads to increased production of UV-B absorbing compounds (i.e. flavonoids) in soybean and alfalfa leaves. This suggests that a protective mechanism in these plants is triggered by UV-B. In response, flavonoids are produced that absorb UV-B, and consequently decrease potentially damaging effects to the plants. In addition, we hypothesize that this flavonoid protection mechanism saturates at certain UV-B intensities.

Levy, Daniel L.↗

Early evaluation of Thematic Mapper data for coastal process studies

Two sets of TM data taken over the ocean off the coast of the Southeastern U.S. Bight were studied for the applicability of TM data to marine environments. First, the results of applying TM and TMS data to determine chlorophyll concentration in the ocean are presented. Chlorophyll quantification in the range of 0.5 to 2.0 mg/cu m was achieved by taking the ratio of TM band-1/band-2. Second, the results of applying TM band-6 data to monitor sea surface temperature are described. A comparison of TM data with AVHRR data shows TM readings coincide with AVHRR data within a scatter of 0.5 deg C in most of the areas studied. Lastly, the results of a technique to map the water depths of coral reefs in the Great Bahama Bank are demonstrated. Depths from 0 to 20 meters were delineated using TM band-1. The classification accuracy and origins of anomalous depth points are discussed.

Kim, H. H.↗

Response of Two Legumes to Two Ultraviolet-B Radiation Regimes

Depletion of the stratospheric ozone layer has been directly linked to increased levels of UV radiation at the earth's surface. The purpose of this study was to evaluate the responses of soybean (Glycine max) and alfalfa (Medicago sativa) to increased UV-B radiation (280-320 nm). Soybean and alfalfa were grown successively in a growth chamber that provided UV-B intensities 45% above nominal summer field levels. Mylar-D (UVB opaque) and mono-acetate (UV-B transparent) films were used to establish the two UV-B treatments. Soybean grown under increased UV showed 21% smaller internodal lengths and higher concentrations of UV-B absorbing pigments (i.e. flavonoids) compared to plants grown under no UV. Significant results for alfalfa included 22% greater leaf flavonoid concentration under increased UV, 14% greater leaf chlorophyll concentration under no UV, and 32% greater above-ground biomass with no UV. These leguminous species possess mechanisms that protect against UV-B damage as indicated by increases in foliar concentrations of UV-B absorbing compounds. Alfalfa appears to be more sensitive to UV-B damage than soybean. Remote sensing of chlorophyll fluorescence may offer a means of monitoring UV-induced plant stress and damage.

Levy, Daniel L.↗

LANDSAT menhaden and thread herring resources investigation

The author has identified the following significant results. The relationship between the distribution of menhaden and selected oceanographic parameters (water color, turbidity, and possibly chlorophyll concentrations) was established. Similar relationships for thread herring were not established nor were relationships relating to the abundance of either species. Use of aircraft and LANDSAT remote sensing instruments to measure or infer a set of basic oceanographic parameters was evaluated. Parameters which could be accurately inferred included surface water temperature, salinity, and color. Water turbidity (Secchi disk) was evaluated as marginally inferrable from the LANDSAT MSS data and chlorophyll-a concentrations as less than marginal. These evaluations considered the parameters only as experienced in the two test areas using available sensors and statistical techniques.

Kemmerer, A. J.↗

Retrieval of Marine Water Constituents Using Atmospherically Corrected AVIRIS Hyperspectral Data

This paper reports on the validation of bio-optical models in estuarine and nearshore (case 2) waters of New Jersey-New York to retrieve accurate water-leaving radiance spectra and chlorophyll concentration from the Airborne Visible/Infrared Imaging Spectrometer (AVIRIS) imaging spectrometer data. MODTRAN-4 was applied to remove the effects of the atmosphere so as to infer the water-leaving radiance. The study area - Hudson/Raritan of New York and New Jersey (Figure 1) is an extremely complex estuarine system where tidal and wind-driven currents are modified by freshwater discharges from the Hudson, Raritan, Hackensack, and Passaic rivers. Over the last century, the estuarine water quality has degraded in part due to eutrophication, which has disrupted the preexisting natural balance, resulting in phytoplankton blooms of both increased frequency and intensity, increasing oxygen demand, and leading to episodes of hypoxia. As the end result, a thematic map of chlorophyll-a concentration was generated using an atmospherically corrected AVIRIS ratio image. This thematic map serves as an indication of phytoplankton concentration. Such maps are important input into the geographic information system (GIS) for use as a management tool for monitoring water resources.

Bagheri, Sima↗

Phytoplankton in the Beaufort and Chukchi Seas: Distributions, Dynamics and Environmental Forcing

Time-series of remotely sensed distributions of phytoplankton, sea ice, surface temperature, albedo, and clouds were examined to evaluate the impact of the variability of environmental conditions and physical forcing on the phytoplankton distribution in the Beaufort and Chukchi Seas. Large-scale distributions of these parameters were studied for the first time using weekly and monthly composites from April 1998 through September 2002. The basic data set used in this study are phytoplankton pigment concentration derived from the Sea-viewing Wide Field-of-view Sensor (SeaWiFS), ice concentration obtained from the Special Sensor Microwave Imager (SSM/I) and surface temperature, cloud cover, and albedo derived from the Advanced Very High Resolution Radiometer (AVHRR). Seasonal variations of the sea ice cover was observed to be the dominant environmental factor as the ice edge blooms followed the retreating marginal ice zones northward. Blooms were most prominent in the southwestern Chukchi Sea, and were especially persistent immediately north of the Bering Strait in nutrient- rich Anadyr water and in some fronts. Chlorophyll concentrations are shown to increase from a nominal value during onset of melt in April to a maximum value in mid-spring or summer depending on location. Large interannual variability of ice cover and phytoplankton distributions was observed with the year 1998 being uniquely associated with an early season occurrence of a massive bloom. This is postulated to be caused in part by a rapid response of phytoplankton to an early retreat of the sea ice cover in the Beaufort Sea region. Correlation analyses showed relatively high negative correlation between chlorophyll and ice concentration with the correlation being highest in May, the correlation coefficient being -0.45. 1998 was also the warmest among the five years globally and the sea ice cover was least extensive in the Beaufort-Khukchi Sea region, partly because of the 1997-98 El Nino. Strong correlations were noted between ice extent and surface temperature, the correlation coefficient being highest at - 0.79 in April, during the onset of the bloom period

Wang, Jian↗

A Passive Method for Detecting Vegetation Stress from Orbit: Chlorophyll Fluorescence Spectra from Fraunhofer Lines

Solar-stimulated chlorophyll fluorescence measured with the Fraunhofer line depth method has correlated well with vegetation stress in previous studies. However, the instruments used in those studies were limited to a single solar absorption line (e.g. 656.3 nm), obviating the red/far-red ratio (R/FR) method. Optics and detector technology have reached the level whereby multiple, very narrow Fraunhofer lines are resolvable. Thirteen such lines span the visible spectrum in the red to far-red region where chlorophyll fluorescence occurs. Fluorescence intensities at the 13 Fraunhofer line wavelengths were used to model emission spectra. The source data were collected for summer and fall bean crops (Phaseolus vulgaris L.) subjected to various levels of nitrogen fertilization. The intensities were adjusted to account for Fraunhofer line depth and atmospheric transmittance. Multiple R/FR fluorescence ratios, calculated from the modeled fluorescence spectra, correlated strongly with leaf chlorophyll concentration and well with applied nitrogen. The ratio yielding the best correlation with chlorophyll utilized red fluorescence at the 694.5 nm Fraunhofer line and farred fluorescence at the 755.6 nm Fraunhofer line. Twenty R/FR ratios, each evaluated for the maximum differential between low and high (optimal) nitrogen treatments, ranked higher in some cases and lower in others, possibly related to the time of year the crops were grown and the stage of growth of the crops. Ratios with 728.9 nm and 738.9 nm in the denominator consistently ranked in the lowest and next lowest quartile, respectively. Ratios of the 656.3 nm Fraunhofer line and the 755.6 nm line consistently ranked highest for the summer crop. Ratios with 755.6 nm in the denominator ranked in the upper quartile for 10 out of 12 measurement dates. Differences in ratio ranking indicate that physiological conditions may be estimated using selected ratios of Fraunhofer lines within the context of R/FR analysis. A passive instrument designed to monitor R/FR chlorophyll fluorescence (i.e. vegetation stress) from orbit could be built today.

Theisen, Arnold F.↗

Azospirillum brasilense Bacteria Promotes Mn2+ Uptake in Maize with Benefits to Leaf Photosynthesis

Azospirillum brasilense is a prolific grass-root colonizing bacteria well-known for its ability to promote plant growth in several cereal crops. Here we show that one of the mechanisms of action in boosting plant performance is through increased assimilation of the micronutrient manganese by the host. Using radioactive 52Mn2+ (t½ 5.59 d), we examined the uptake kinetics of this micronutrient in young maize plants, comparing the performance of three functional mutants of A. brasilense, including HM053, a high auxin-producing and high N2-fixing strain; ipdC, a strain with a reduced auxin biosynthesis capacity; and FP10, a strain deficient in N2-fixation that still produces auxin. HM053 had the greatest effect on host 52Mn2+ uptake, with a significant increase seen in shoot radioactivity relative to non-inoculated controls. LA-ICP-MS analysis of root sections revealed higher manganese distributions in the endodermis of HM053-inoculated plants and overall higher manganese concentrations in leaves. Finally, increased leaf manganese concentration stimulated photosynthesis as determined by measuring leaf fixation of radioactive 11CO2 with commensurate increases in chlorophyll concentration.

59 BASIC BIOLOGICAL SCIENCES↗

The blue-to-green reflectance ratio and lake water quality

Correlations between the relative values of the blue and green reflectances of a lake and water quality indices, such as depth of photic zone, Secchi disk transparency, attenuation coefficient, and chlorophyll concentration, have been observed during an intensive satellite, aircraft, and surface vessel study of Lake Ontario and Conesus Lake. Determinations of blue and green reflectances from Skylab S190A color imagery are in excellent agreement with values obtained from small-scale color imagery from aircraft. Further, the accuracy of the satellite data appears within that required for extrapolation to the water quality indices. The study has also determined that changes in chlorophyll, lignin, and humic acid concentration can be discriminated by the behavior of the blue-to-green reflectance ratio and the reflectances of the green and red bands.

Piech, K. R.↗

Active-passive airborne ocean color measurement. II - Applications

Reported here for the first time is the use of a single airborne instrument to make concurrent measurements of oceanic chlorophyll concentration by (1) laser-induced fluorescence, (2) passive upwelling radiance, and (3) solar-induced chlorophyll fluorescence. Results from field experiments conducted with the NASA airborne oceanographic lidar (AOL) in the New York Bight demonstrate the capability of a single active-passive instrument to perform new and potentially important ocean color studies related to (1) active lidar validation of passive ocean color in-water algorithms, (2) chlorophyll a in vivo fluorescence yield variability, (3) calibration of active multichannel lidar systems, (4) effect of sea state on passive and active ocean color measurements, (5) laser/solar-induced chlorophyll fluorescence investigations, and (6) subsequent improvement of satellite-borne ocean color scanners. For validation and comparison purposes a separate passive ocean color sensor was also flown along with the new active-passive sensor during these initial field trials.

Hoge, F. E.↗

Estimation of the processes controlling variability in phytoplankton pigment distributions on the southeastern U.S. continental shelf

Five coastal-zone-color-scanner images from the southeastern U.S. continental shelf are combined with concurrent moored current meter measurements to assess the processes controlling the variability in chlorophyll concentration and distribution in this region. An equation governing the space and time distribution of a nonconservative quantity such as chlorophyll is used in the calculations. The terms of the equation, estimated from observations, show that advective, diffusive, and local processes contribute to the plankton distributions and vary with time and location. The results from this calculation are compared with similar results obtained using a numerical physical-biological model with circulation fields derived from an optimal interpolation of the current meter observations and it is concluded that the two approaches produce different estimates of the processes controlling phytoplankton variability.

Mcclain, Charles R.↗