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At least 91 records · Page 5

A Tale of Two Catchments: Causality Analysis and Isotope Systematics Reveal Mountainous Watershed Traits That Regulate the Retention and Release of Nitrogen

Abstract Mountainous watersheds are characterized by variability in functional traits, including vegetation, topography, geology, and geomorphology, which determine nitrogen (N) retention, and release. Coal Creek and East River are two contrasting catchments within the Upper Colorado River Basin that differ markedly in total nitrate (NO 3 − ) export. The East River has a diverse vegetation cover, and sinuous floodplains, and is underlain by N‐rich marine shale. At 0.21 ± 0.14 kg ha −1 yr −1 , the East River exports ∼3.5 times more NO 3 − relative to the conifer‐dominated Coal Creek (0.06 ± 0.02 kg ha −1 yr −1 ). While this can partly be explained by the larger size of the East River, the distinct watershed traits of these two catchments imply different mechanisms controlling the aggregate N‐export signal. A causality analysis shows physical and biogenic processes were critical in determining NO 3 − export from the East River catchment. Stable isotope ratios of NO 3 − (δ 15 N NO3 and δ 18 O NO3 ) show the East River catchment is a strong hotspot for biogeochemical processing of NO 3 − at the hillslope soil‐saprolite. By contrast, the conifer‐dominated Coal Creek retained nearly all atmospherically deposited NO 3 − , and its export was controlled by catchment hydrological traits (i.e., snowmelt periods and water table depth). The conservative N‐cycle within Coal Creek is likely due to the abundance of conifer trees, and smaller riparian regions, retaining more NO 3 − overall and reduced processing prior to export. This study highlights the value of integrating isotope systematics to link watershed functional traits to mechanisms of watershed element retention and release.

54 ENVIRONMENTAL SCIENCES↗

Investigating Coastal Vegetation Dynamics and Ecosystem Impacts Under Elevated CO 2 and Temperature: A Process‐Based Approach

Coastal forests are increasingly vulnerable to climate change and sea-level rise, with flooding and salinity driving transitions to marsh-dominated ecosystems. Using the coastal version of FATES-Hydro, we conducted 30-year simulations at two coastal forest sites—a broadleaf swamp white oak stand at Lake Erie and a conifer loblolly pine stand at Chesapeake Bay—under historical climate and elevated CO 2 (+100 ppm) and temperature (+1.5°C) scenarios. Elevated CO 2 increased net primary productivity at both sites, while warming alone intensified hydraulic stress and accelerated mortality, particularly in the conifer stand. Simulations show that elevated temperatures intensify vapor pressure deficit and hydraulic stress on trees already experiencing salinity- and submersion-driven water stress, increasing tree mortality beyond what would be expected in a non-water-limited environment. Marsh expansion partially compensated for tree loss at the Lake Erie site but reduced ecosystem productivity in the conifer forest at Chesapeake Bay. In conclusion, our results highlight how differences in stand structure, phenology, and local hydrology modulate ecosystem trajectories under climate change, emphasizing the importance of demographic and community-level processes for predicting the fate of coastal forests.

Ding, Junyan [Barcelona Supercomputing Center (BSC↗

Deep-learning-based canopy height model generation from sub-meter resolution panchromatic satellite imagery

Canopy height models (CHMs) with sufficient resolution to distinguish individual trees are useful for a variety of applications. However, standard techniques to acquire such data, such as airborne lidar surveying, are often prohibitively expensive. Deep learning techniques for generating CHMs from high-resolution imagery are an attractive option to reduce costs. To date, success with these methods has been demonstrated using multichannel aerial photography and specialized satellite data products derived from multiple sensors, neither of which is commonly available at temporal resolutions finer than one year. Here we demonstrate a method to generate sub-meter resolution CHMs in three forests in California using a more abundant data source: sub-meter resolution, panchromatic satellite imagery from a single sensor. We show that phenology and species composition play important roles in model transferability; when trained using imagery from a single conifer forest in autumn, the model performs well on autumn imagery from a second conifer forest several hundred kilometers distant with no re-training. With modest additions to the training dataset, the same model generates minimally biased estimates of canopy height in both conifer and deciduous forests during multiple seasons. Because the model operates on satellite data with global coverage and a relatively short return interval, we propose its suitability to extrapolate tree-level canopy height data to remote regions and conduct high-temporal resolution monitoring of forest structure. We furthermore demonstrate the workflow’s applicability to fire modeling by conducting simulations in forests populated by trees measured using both this approach and airborne lidar surveying. We find minimal differences in fire behavior relative to a baseline case in which only statistical distributions of tree height and crown area are known. This result underscores the value of forest structural information derived from our workflow for improving the fidelity of wildland fire simulations, among other ecological applications.

54 ENVIRONMENTAL SCIENCES↗

CO2 refixation is higher in leaves of woody species with high mesophyll and stomatal resistances to CO2 diffusion

Abstract The percentage of respiratory and photorespiratory CO2 refixed in leaves (Pr) represents part of the CO2 used in photosynthesis. The importance of Pr as well as differences between species and functional types are still not well investigated. In this study, we examine how Pr differs between six temperate and boreal woody species: Betula pendula, Quercus robur, Larix decidua, Pinus sylvestris, Picea abies and Vaccinium vitis-idaea. The study covers early and late successional species, deciduous broadleaves, deciduous conifers, evergreen conifers and evergreen broadleaves. We investigated whether some species or functional types had higher refixation percentages than others, whether leaf traits could predict higher Pr and whether these traits and their impact on Pr changed during growing seasons. Photosynthesis CO2 response (A/Ci)-curves, measured early, mid and late season, were used to estimate and compare Pr, mesophyll resistance (rm) and stomatal resistance (rs) to CO2 diffusion. Additionally, light images and transmission electron microscope images were used to approximate the fraction of intercellular airspace and cell wall thickness. We found that evergreens, especially late successional species, refixed a significantly higher amount of CO2 than the other species throughout the entire growing season. In addition, rm, rs and leaf mass per area, traits that typically are higher in evergreen species, were also significantly, positively correlated with Pr. We suggest that this is due to higher rm decreasing diffusion of (photo) respiratory CO2 out of the leaf. Cell wall thickness had a positive effect on Pr and rm, while the fraction of intercellular airspace had no effect. Both were significantly different between evergreen conifers and other types. Our findings suggest that species with a higher rm use a greater fraction of mitochondria-derived CO2, especially when stomatal conductance is low. This should be taken into account when modeling the overall CO2 fertilization effect for terrestrial ecosystems dominated by high rm species.

59 BASIC BIOLOGICAL SCIENCES↗

Effect of gap size on composition and structure of regeneration 19 years after harvest in a southeastern bottomland forest, USA

Following timber harvests in bottomland mixed-oak (Quercus L.) stands, desirable oak advance regeneration can be overgrown by shade-intolerant pioneer species. We investigated the effects of group selection opening size on composition of tree regeneration 19 years postharvest in an oak-dominated bottomland forest and compared results with earlier findings to evaluate development trends. In response to six opening sizes (7–40 m radii), we evaluated regeneration density and dominance of four tree species groups: conifers, hard mast, shade-intolerant hardwoods, and shade-tolerant hardwoods. Our objectives were to determine the optimum gap size for regenerating oaks and test the delayed oak dominance hypothesis, in which oaks slowly gain dominance as pioneer species undergo self-thinning. Opening size influenced conifer regeneration but minimally affected hardwoods. Hard mast species density was less than that of either intolerant or tolerant species regardless of opening size. Future stem density trends suggest increasing intolerant species and constant mast and tolerant species. Modeled future height trends suggest increasing mast species dominance over intolerant pioneers after 30 years. Furthermore, our results suggest that gap-based silviculture can be used to regenerate bottomland hardwood stands with desirable species including oaks; larger gaps favor conifers but there was no optimum size to enhance oak regeneration.

60 APPLIED LIFE SCIENCES↗

Raw Data published in PeerJ, 2021 for Crested Butte decomposition field study 2017-2019.

This data package contains text files that describe geochemical measurements collected from 2017-2019 during isolated conifer needle decomposition field studies in Crested Butte, Colorado. The geochemical measurements were collected across three elevations (2,800–3,500 m) ranging from montane to subalpine ecoregions. The data sets within include total carbon and nitrogen content and fourier-transform infrared spectroscopy (FTIR) results for the initial needles collected in 2016 and after decomposition in 2019. Data collection results from August 2020 are also included from when the experimental plots were removed to understand final concentrations of soil extractable carbon and nitrogen content as well as mass balances from litter bag deployments. Soil porewater results are also provided from 2017-2019 for DOC, TN, UV254, and specific UV absorbance (SUVA) analyses at 15 cm soil depth. Gas flux raw data provides CO2, CH4, N2O, and NH3 measurements above needle decomposition over the three study years. Finally, soil samples for microbial DNA extractions were collected from the upper soil depth. This raw data is available in the NCBI SRA database under SRA accession numbers PRJNA605259 and PRJNA715914. These data sets were generated to investigate the isolated decomposition of spruce and lodgepole conifer needles. The goal of this work was to determine the roles of elevation, soil type, seasonal changes in soil moisture, and snowmelt timing on litter decomposition processes. Results from this work are detailed in the reference paper "Effect of elevation, season and accelerated snowmelt on biogeochemical processes during isolated conifer needle litter decomposition. DOI: 10.7717/peerj.11926."

54 ENVIRONMENTAL SCIENCES↗

Small Mammal Prey Study for the Mexican Spotted Owl at Two Locations at Los Alamos National Laboratory

In New Mexico, Mexican spotted owls (Strix occidentalis lucida) are typically found in rocky canyons consisting of mixed-conifer forests that have experienced minimal human disturbance. At Los Alamos National Laboratory (LANL), Mexican spotted owls have been found breeding and foraging in habitats consistent with their known biology, but face the impact of human encroachment in the surrounding upland habitat. Little is known about the prey base present within the Laboratory’s vast forests. In anticipation of expanding human development in proximity to known occupied owl habitat, the goal of our study was to evaluate the small mammal prey base available to Mexican spotted owls at the Laboratory and to assess prey availability, diversity and composition. We sampled two study plots within the Laboratory’s forests for small mammals in 2021. Study plots were situated in conifer, deciduous, mixed conifer-deciduous and mixed oak. Our small mammal trapping efforts and SCR modeling revealed site-specific differences in prey base diversity, abundance and density between the unoccupied Pajarito Canyon and nearby occupied Mortandad Canyon site. These results highlight how managing tracts of land for small mammal prey base may overlap with goals set forth by researchers for Mexican spotted owl habitat needs. We recommend further trapping efforts to better understand prey availability in occupied and unoccupied sites at LANL as well as further investigation of prey selection through the dissection of pellets found in the areas surrounding known nesting locations.

54 ENVIRONMENTAL SCIENCES↗

Detection of aspen/conifer forest mixes from multitemporal Landsat digital data

Aspen, conifer and mixed aspen/conifer forests were mapped for a 15-quadrangle study area in the Utah-Idaho Bear River Range using Landsat multispectral scanner data. Digital classification and statistical analysis of Landsat data allowed the identification of six groups of signatures which reflect different types of aspen/conifer forest mixing. Photo interpretations of the print symbols suggest that such classes are indicative of mid to late seral aspen forests. Digital print map overlayes and acreage calculations were prepared for the study area quadrangles. Further field verification is needed to acquire additional information about the nature of the forests. Single data Landsat analysis should be a cost effective means to index aspen forests which are at least in the mid seral phase of conifer invasion. Since aspen canopies tend to obscure understory conifers for early seral forests, a second data analysis, using data taken when aspens are leafless, could provide information about early seral aspen forests.

Merola, J. A.↗

Early remote laser detection of vegetation damage caused by certain environmental stress factors

The fluorescence spectra of plants excited with a pulsed nitrogen laser beam emitting at 337 nm were found to be related to plant type, as well as with changes in the physiology of the plant as the result of various kinds of environmental stress. The plant types which were studied included herbaceous dicots, monocots, hardwoods, and conifers. These plant types could be identified on the basis of differences in either the number of fluorescent bands, or the relative intensity of the bands. The dicots and monocots had fluorescent maxima at 440, 685, and 740 nm. The monocots could be distinguished from the dicots by virtue of having a much higher 440 nm/685 nm ratio. Hardwoods and conifers had an additional fluorescence band at 525 nm, but healthy conifers did not have a band at 685 nm.

Chappelle, Emmett W.↗

Needle bacterial community structure across the species range of limber pine

Abstract Bacteria on and inside leaves can influence forest tree health and resilience. The distribution and limits of a tree species’ range can be influenced by various factors, with biological interactions among the most significant. We investigated the processes shaping the bacterial needle community across the species distribution of limber pine, a widespread Western conifer inhabiting a range of extreme habitats. We tested four hypotheses: (i) Needle community structure varies across sites, with site-specific factors more important to microbial assembly than host species selection; (ii) dispersal limitation structures foliar communities across the range of limber pine; (iii) the relative significance of dispersal and selection differs across sites in the tree species range; and (iv) needle age structures bacterial communities. We characterized needle communities from the needle surface and tissue of limber pine and co-occurring conifers across 16 sites in the limber pine distribution. Our findings confirmed that site characteristics shape the assembly of bacterial communities across the host species range and showed that these patterns are not driven by dispersal limitation. Furthermore, the strength of selection by the host varied by site, possibly due to differences in available microbes. Our study, by focusing on trees in their natural setting, reveals real needle bacterial dynamics in forests, which is key to understanding the balance between stochastic and deterministic processes in shaping forest tree-microbe interactions. Such understanding will be necessary to predict or manipulate these interactions to support forest ecosystem productivity or assist plant migration and adaptation in the face of global change.

59 BASIC BIOLOGICAL SCIENCES↗

SPRUCE Photosynthesis and Respiration of Picea mariana and Larix laricina in SPRUCE Experimental Plots, 2016-2017

This dataset contains empirical physiological, morphological, and chemical data collected on two dominant conifer species, Picea mariana and Larix laricina, between May 2016 and August 2017 at the SPRUCE (Spruce and Peatland Responses Under Changing Environments) experimental site within the Marcell Experimental Forest in northern Minnesota, USA. Analyses assessed the response of conifers to whole-ecosystem warming and elevated atmospheric carbon dioxide (CO2) concentrations. Measurements include light-saturated net photosynthesis and dark respiration at ambient atmospheric carbon dioxide (CO2) concentration (2016), CO2 response curves of light-saturated net photosynthesis (2017), leaf morphology (leaf mass per unit leaf area) and nitrogen content (on both mass and area basis). Leaf photosynthesis and respiration were measured with portable photosynthesis systems (LI6400XT, LI6800, LI-COR Biosciences, USA). In 2016, photosynthesis and respiration were measured at a common temperature of 25 ˚C to assess thermal acclimation, and at growth temperatures (for each of the five treatment temperatures) to assess field performance. In 2017, CO2 response curves of net photosynthesis (so-called A-Ci curves) were conducted at different leaf temperatures (15, 25, 32.5, 40 and 45 ˚C) for assessment of biochemical parameters and their temperature sensitivity. In addition, dark respiration measurements at different leaf temperatures (10, 15, 20, 25, 30, 35, 40, and 45 ºC) were also taken. This dataset contains one data file in comma-separate values (*.csv) and Microsoft Excel (*.xlsx) format. Additional metadata are provided: a data dictionary and a file-level metadata file in comma-separate values (.csv) format and a user guide in PDF (*.pdf) format.

54 ENVIRONMENTAL SCIENCES↗

Climate-Induced Mortality of Siberian Pine and Fir in the Lake Baikal Watershed, Siberia

Siberian pine (Pinus sibirica) and fir (Abies sibirica) (so called ''dark needle conifers", DNC) showed decreased radial growth increment within the Lake Baikal watershed since the 1980s with increasing mortality recorded since the year 2000. Tree ring width was strongly correlated with vapor pressure deficit, aridity and root zone moisture. Water stress from droughts made trees more susceptible to insect attacks causing mortality in about 10% of DNC stands within the Lake Baikal watershed. Within Siberia DNC mortality increased in the southern part of the DNC range. Biogeographically, tree mortality was located within the DNC - forest-steppes transition. Tree mortality was significantly correlated with drought and soil moisture anomalies. Within the interior of the DNC range mortality occurred within relief features with high water stress risk (i.e., steep convex south facing slopes with shallow well-drained soils). In general, DNC mortality in Siberia was induced by increased aridity and severe drought (inciting factors) in synergy with biotic attacks (contributing factor). In future climate scenarios with predicted increase in aridity DNC could be eliminated from the southern part of its current range and will be replaced by drought-resistant conifers and broadleaf species (e.g., Larix sibirica, Pinus silvestris, and Betula pubescence).

Conifer Mortality↗

Climate Warming Alters Nutrient Storage in Seasonally Dry Forests: Insights From a 2,300 m Elevation Gradient

Understanding potential response of forest carbon (C) and nutrient storage to warming is important for climate mitigation policies. Unfortunately, those responses are difficult to predict in seasonally dry forests, in part, because ecosystem processes are highly sensitive to both changes in temperature and precipitation. We investigated how warming might alter stocks of C, nitrogen (N), and phosphorus (P) in vegetation and the entire regolith (soil + weathered bedrock or “saprock”) using a space-for-time substitution along a bioclimatic gradient in the Sierra Nevada, California. The pine-oak and mixed-conifer forests between 1,160–2,015 m elevation have more optimal climates (not too dry or hot) for ecosystem productivity, soil weathering, and cycling of essential elements than the oak savannah (405 m) and subalpine forest (2,700 m). We found decreases in overstory vegetation nutrient stocks with decreasing elevation because of enhanced water limitation and greater occurrence of disturbances. Stocks of C, N, and P in the entire regolith peaked at the pine-oak and mixed-conifer forests across the bioclimatic gradient, driven by thicker regolith profiles and greater nutrient input rates. These observations suggest long-term warming will decrease ecosystem nutrient storage at the warmer, transitional pine-oak zone, but will increase nutrient storage at the colder, subalpine zone. Assuming steady-state conditions, we found the mean residence time of ecosystem C decreased with projected rising air temperatures and increased following a major drought event across the bioclimatic gradient. Our study emphasizes potentially elevation-dependent changes in nutrient storage and C persistence with warming in seasonally dry forests.

54 ENVIRONMENTAL SCIENCES↗

Omics analyses and biochemical study of Phlebiopsis gigantea elucidate its degradation strategy of wood extractives

Wood extractives, solvent-soluble fractions of woody biomass, are considered to be a factor impeding or excluding fungal colonization on the freshly harvested conifers. Among wood decay fungi, the basidiomycete Phlebiopsis gigantea has evolved a unique enzyme system to efficiently transform or degrade conifer extractives but little is known about the mechanism(s). In this study, to clarify the mechanism(s) of softwood degradation, we examined the transcriptome, proteome, and metabolome of P. gigantea when grown on defined media containing microcrystalline cellulose and pine sapwood extractives. Beyond the conventional enzymes often associated with cellulose, hemicellulose and lignin degradation, an array of enzymes implicated in the metabolism of softwood lipophilic extractives such as fatty and resin acids, steroids and glycerides was significantly up-regulated. Among these, a highly expressed and inducible lipase is likely responsible for lipophilic extractive degradation, based on its extracellular location and our characterization of the recombinant enzyme. Our results provide insight into physiological roles of extractives in the interaction between wood and fungi.

59 BASIC BIOLOGICAL SCIENCES↗

Raw Data published in PeerJ, 2020 for Lower Subalpine needle decomposition field study 2017-2018

This data package contains text files that describe geochemical measurements collected from 2017-2018 during isolated conifer needle decomposition field studies at the Lower Subalpine region of Washington Gulch in Crested Butte, CO. The files within include total carbon and nitrogen (CN) content and fourier-transform infrared spectroscopy (FTIR) results for the initial needles collected in 2016 and after decomposition in October 2018. DI water extractions were conducted with the initial harvested needles in 2016 to determine extractable dissolved organic carbon (DOC), total nitrogen (TN), UV absorbance at 254 nm (UV254), and specific UV absorbance (SUVA). This dataset also includes soil extractable DOC, TN, nitrate, nitrite, ammonium, total organic nitrogen (TON), and pH results from soil collections in 2017. Soil porewater results for DOC, TN, UV254, and SUVA analyses at 15 cm soil depth are also provided from 2017-2018. Gas flux raw data provides CO2, CH4, N2O, and NH3 measurements above needle decomposition over the two study years. Finally, soil samples for microbial DNA extractions were collected from the upper soil depth. This raw data is available in the NCBI SRA database under SRA accession number PRJNA605259. These datasets were generated to investigate the isolated decomposition of spruce and lodgepole conifer needles. The goal of this work was to understand how needle litter impacts terrestrial biogeochemical carbon and nitrogen cycling. Results from this work is detailed in the reference paper "A comparison of lodgepole and spruce needle chemistry impacts on terrestrial biogeochemical processes during isolated needle decomposition. DOI: 10.7717/peerj.9538."

54 ENVIRONMENTAL SCIENCES↗

Small Mammal Prey Base for American Marten (Martes americana) within the Manistee National Forest of Michigan

American martens (Martes americana) are typically found in late-successional forests with closed canopy cover and high structural diversity. Reintroduced populations of martens in the Northern Lower Peninsula of Michigan inhabit areas that are devoid of many of these features, which may impact their prey base. The goal of our study was to evaluate the small mammal prey base available to martens in the Northern Lower Peninsula. To assess prey availability, diversity, and composition, as well as effects of trap type and habitat features on trapping success, we sampled 24 study plots within the Manistee National Forest for small mammals in 2013 (n=24) and 2014 (n=20). Study plots were situated in four habitat types: conifer, deciduous, mixed conifer-deciduous, and mixed oak. Total capture rates were significantly and positively associated with relative deciduous tree cover. Furthermore, this result highlights how managing tracts of land for small mammal prey base may overlap with goals set forth by researchers for marten habitat needs (e.g., resting site preferences). We found large Sherman traps had significantly higher total capture rates than other trap types (medium Sherman and pitfall traps), and we recommend that researchers use a variety of trap types to maximize detectability of small mammal species diversity and richness.

59 BASIC BIOLOGICAL SCIENCES↗

AmeriFlux US-PFj NE4 Maple-1 CHEESEHEAD 2019

This is the AmeriFlux version of the carbon flux data for the site US-PFj NE4 Maple-1 CHEESEHEAD 2019. Site Description - This tower (32m, trailer-based, telescoping) is located in the northeastern quadrant of the 10 x 10km study domain. It is located in a maple forest (canopy height: 18 - 20m). Some conifers mixed in, more conifers to the NNW.

Desai, Ankur↗

AmeriFlux FLUXNET-1F CA-TPD Ontario - Turkey Point Mature Deciduous

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site CA-TPD Ontario - Turkey Point Mature Deciduous. This is the FLUXNET version of the carbon flux data for the site CA-TPD Ontario - Turkey Point Mature Deciduous produced by applying the standard ONEFlux (1F) software. Site Description - The forest is approximately 90 years old. Naturally regenerated on sandy terrain and abandoned agricultural land. Predominantly hardwood species with a few scattered conifers. Site has been managed (thinned) in the past. It has a high biodiversity with 573 tree and plant species, 102 bird species, 23 mamal species and 22 reptile and amphibian species (SWALSREP Report, 1999). The dominant tree species is white oak (Quercus alba), with other scattered broadleaf Carolinian species including sugar and red maple (Acer saccharum, A. rubrum), American beech (Fagus grandifolia), black and red oak (Q. velutina, Q. rubra) and white ash (Fraxinus americana) . There are also scattered conifers, mostly white and red pine (Pinus strobes, P. resinosa), comprising about 5% of the trees. Average tree height is 25.7 m with a stand density of 504 ± 18 trees per hectare. Average tree diameter at breast height is 22.3 cm and basal area is 0.06 m2 or approximately 29 square meters per hectare.

Arain, M. Altaf↗