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At least 91 records · Page 5

Results from a multi-laboratory ocean metaproteomic intercomparison: effects of LC-MS acquisition and data analysis procedures

Metaproteomics is an increasingly popular methodology that provides information regarding the metabolic functions of specific microbial taxa and has potential for contributing to ocean ecology and biogeochemical studies. A blinded multi-laboratory intercomparison was conducted to assess comparability and reproducibility of taxonomic and functional results and their sensitivity to methodological variables. Euphotic zone samples from the Bermuda Atlantic Time-series Study (BATS) in the North Atlantic Ocean collected by in situ pumps and the autonomous underwater vehicle (AUV) Clio were distributed with a paired metagenome, and one-dimensional (1D) liquid chromatographic data-dependent acquisition mass spectrometry analysis was stipulated. Analysis of mass spectra from seven laboratories through a common bioinformatic pipeline identified a shared set of 1056 proteins from 1395 shared peptide constituents. Quantitative analyses showed good reproducibility: pairwise regressions of spectral counts between laboratories yielded R 2 values averaged 0.62±0.11, and a Sørensen similarity analysis of the top 1000 proteins revealed 70 %–80 % similarity between laboratory groups. Taxonomic and functional assignments showed good coherence between technical replicates and different laboratories. A bioinformatic intercomparison study, involving 10 laboratories using eight software packages, successfully identified thousands of peptides within the complex metaproteomic datasets, demonstrating the utility of these software tools for ocean metaproteomic research. Lessons learned and potential improvements in methods were described. Future efforts could examine reproducibility in deeper metaproteomes, examine accuracy in targeted absolute quantitation analyses, and develop standards for data output formats to improve data interoperability. Together, these results demonstrate the reproducibility of metaproteomic analyses and their suitability for microbial oceanography research, including integration into global-scale ocean surveys and ocean biogeochemical models.

59 BASIC BIOLOGICAL SCIENCES↗

SPEX Airborne Spectropolarimeter Calibration and Performance

To improve our understanding of the complex role of aerosols in the climate system and on air quality, measurements are needed of optical and microphysical aerosol. From many studies, it has become evident that a satellite-based multiangle, multiwavelength polarimeter will be essential to provide such measurements. Here, high accuracy (∼0.003) on the degree of linear polarization (DoLP) measurements is important to retrieve aerosol properties with an accuracy needed to advance our understanding of the aerosol effect on climate. SPEX airborne, a multiangle hyperspectral polarimeter, has been developed for observing and characterizing aerosols from NASA’s high-altitude research aircraft ER-2. It delivers measurements of radiance and DoLP at visual wavelengths with a spectral resolution of 3 and 7–30 nm, respectively, for radiance and polarization, at nine fixed equidistant viewing angles from −56° to +56° oriented along the ground track, and a swath of 7° oriented across-track. SPEX airborne uses spectral polarization modulation to determine the state of linear polarization of scattered sunlight. This technique has been developed in the Netherlands and has been demonstrated with ground-based instruments. SPEX airborne serves as a demonstrator for a family of space-based SPEX instruments that have the ability to measure and characterize atmospheric aerosol by multiangle hyperspectral polarimetric imaging remotely from a satellite platform. SPEX airborne was calibrated radiometrically and polarimetrically using Jet Propulsion Laboratory (JPL) facilities including the Polarization Stage Generator-2 (PSG-2), which is designed for polarimetric calibration and validation of the Airborne Multiangle SpectroPolarimetric Imager (AirMSPI). Using the PSG-2, the accuracy of the SPEX airborne DoLP measurements in the laboratory setup is found to be 0.002–0.004. Radiometric calibration is realized with an estimated accuracy of 4%. In 2017, SPEX airborne took part in the “Aerosol Characterization from Polarimeters and Lidar” campaign on the ER-2 that included four polarimeters and two lidars. Polarization measurements of SPEX airborne and the coflying Research Scanning Polarimeter (RSP), recorded during the campaign, were compared and display root-mean-square (RMS) differences ranging from 0.004 (at 555 nm) up to 0.02 (at 410 nm). For radiance measurements, excellent agreement between SPEX airborne and RSP is obtained with an RMS difference of ∼4%. The lab- and flight-performance values for polarization are similar to those recently published for AirMSPI, where also an intercomparison with RSP was made using data from field campaigns in 2013. The intercomparison of radiometric and polarimetric data both display negligible bias. The in-flight comparison results provide verification of SPEX airborne’s capability to deliver high-quality data.

Aerosols↗

Characteristics of space and ground based total ozone observing systems investigated by intercomparison of Nimbus 4 Backscattered Ultraviolet /BUV/ data with Dobson and M-83 results

The Nimbus 4 Backscattered Ultraviolet (BUV) experiment data has been reprocessed to provide a near global data set covering the seven years from 1970 to 1977. The data set is the result of a three year effort by the Ozone Processing Team which included review and correction of the spacecraft ephemeris, instrument calibration, and processing algorithms. The accuracy of the resulting total ozone determinations compares well with that of ground based data. An important application of the global BUV data set is to use it in determining long term trends in global total ozone. However, in order to identify long term trends in ozone, it is necessary to eliminate first long-term drift in the instrument calibration. A follow on experiment, the Solar Backscattered Ultraviolet/Total Ozone Mapping Spectrometer, (SBUV/TOMS), was flown on Nimbus 7 launched in October 1978. The SBUV provides information about both total ozone and vertical ozone profiles for a 200 km square field of view.

Fleig, A. J.↗

Metafitting: Weight optimization for least-squares fitting of PTTI data

For precise time intercomparisons between a master frequency standard and a slave time scale, we have found it useful to quantitatively compare different fitting strategies by examining the standard uncertainty in time or average frequency. It is particularly useful when designing procedures which use intermittent intercomparisons, with some parameterized fit used to interpolate or extrapolate from the calibrating intercomparisons. We use the term 'metafitting' for the choices that are made before a fitting procedure is operationally adopted. We present methods for calculating the standard uncertainty for general, weighted least-squares fits and a method for optimizing these weights for a general noise model suitable for many PTTI applications. We present the results of the metafitting of procedures for the use of a regular schedule of (hypothetical) high-accuracy frequency calibration of a maser time scale. We have identified a cumulative series of improvements that give a significant reduction of the expected standard uncertainty, compared to the simplest procedure of resetting the maser synthesizer after each calibration. The metafitting improvements presented include the optimum choice of weights for the calibration runs, optimized over a period of a week or 10 days.

Douglas, Rob J.↗

Validation of AIRS V6 Surface Temperature over Greenland with GCN and NOAA Stations

This work compares the temporal and spatial characteristics of the AIRSAMSU (Atmospheric Infrared Sounder Advanced Microwave Sounding Unit A) Version 6 and MODIS (Moderate resolution Imaging Spectroradiometer) Collection 5 derived surface temperatures over Greenland. To estimate uncertainties in space-based surface temperature measurements, we re-projected the MODIS Ice Surface Temperature (IST) to 0.5 by 0.5 degree spatial resolution. We also re-gridded AIRS Skin Temperature (Ts) into the same grid but classified with different cloud conditions and surface types. These co-located data sets make intercomparison between the two instruments relatively straightforward. Using this approach, the spatial comparison between the monthly mean AIRS Ts and MODIS IST is in good agreement with RMS 2K for May 2012. This approach also allows the detection of any long-term calibration drift and the careful examination of calibration consistency in the MODIS and AIRS temperature data record. The temporal correlations between temperature data are also compared with those from in-situ measurements from GC-Net (GCN) and NOAA stations. The coherent time series of surface temperature evident in the correlation between AIRS Ts and GCN temperatures suggest that at monthly time scales both observations capture the same climate signal over Greenland. It is also suggested that AIRS surface air temperature (Ta) can be used to estimate the boundary layer inversion.

AIRS↗

The Fifth Calibration/Data Product Validation Panel Meeting

The minutes and associated documents prepared from presentations and meetings at the Fifth Calibration/Data Product Validation Panel meeting in Boulder, Colorado, April 8 - 10, 1992, are presented. Key issues include (1) statistical characterization of data sets: finding statistics that characterize key attributes of the data sets, and defining ways to characterize the comparisons among data sets; (2) selection of specific intercomparison exercises: selecting characteristic spatial and temporal regions for intercomparisons, and impact of validation exercises on the logistics of current and planned field campaigns and model runs; and (3) preparation of data sets for intercomparisons: characterization of assumptions, transportable data formats, labeling data files, content of data sets, and data storage and distribution (EOSDIS interface).

Source record↗

Characterization Approaches to Place Invariant Sites on SI-Traceable Scales

The effort to understand the Earth's climate system requires a complete integration of remote sensing imager data across time and multiple countries. Such an integration necessarily requires ensuring inter-consistency between multiple sensors to create the data sets needed to understand the climate system. Past efforts at inter-consistency have forced agreement between two sensors using sources that are viewed by both sensors at nearly the same time, and thus tend to be near polar regions over snow and ice. The current work describes a method that would provide an absolute radiometric calibration of a sensor rather than an inter-consistency of a sensor relative to another. The approach also relies on defensible error budgets that eventually provides a cross comparison of sensors without systematic errors. The basis of the technique is a model-based, SI-traceable prediction of at-sensor radiance over selected sites. The predicted radiance would be valid for arbitrary view and illumination angles and for any date of interest that is dominated by clear-sky conditions. The effort effectively works to characterize the sites as sources with known top-of-atmosphere radiance allowing accurate intercomparison of sensor data that without the need for coincident views. Data from the Advanced Spaceborne Thermal Emission and Reflection and Radiometer (ASTER), Enhanced Thematic Mapper Plus (ETM+), and Moderate Resolution Imaging Spectroradiometer (MODIS) are used to demonstrate the difficulties of cross calibration as applied to current sensors. Special attention is given to the differences caused in the cross-comparison of sensors in radiance space as opposed to reflectance space. The radiance comparisons lead to significant differences created by the specific solar model used for each sensor. The paper also proposes methods to mitigate the largest error sources in future systems. The results from these historical intercomparisons provide the basis for a set of recommendations to ensure future SI-traceable cross calibration using future missions such as CLARREO and TRUTHS. The paper describes a proposed approach that relies on model-based, SI-traceable predictions of at-sensor radiance over selected sites. The predicted radiance would be valid for arbitrary view and illumination angles and for any date of interest that is dominated by clear-sky conditions. The basis of the method is highly accurate measurements of at-sensor radiance of sufficient quality to understand the spectral and BRDF characteristics of the site and sufficient historical data to develop an understanding of temporal effects from changing surface and atmospheric conditions.

Thome, Kurtis↗

Analysis of airborne Doppler lidar, Doppler radar and tall tower measurements of atmospheric flows in quiescent and stormy weather

The first experiment to combine airborne Doppler Lidar and ground-based dual Doppler Radar measurements of wind to detail the lower tropospheric flows in quiescent and stormy weather was conducted in central Oklahoma during four days in June-July 1981. Data from these unique remote sensing instruments, coupled with data from conventional in-situ facilities, i.e., 500-m meteorological tower, rawinsonde, and surface based sensors, were analyzed to enhance understanding of wind, waves and turbulence. The purposes of the study were to: (1) compare winds mapped by ground-based dual Doppler radars, airborne Doppler lidar, and anemometers on a tower; (2) compare measured atmospheric boundary layer flow with flows predicted by theoretical models; (3) investigate the kinematic structure of air mass boundaries that precede the development of severe storms; and (4) study the kinematic structure of thunderstorm phenomena (downdrafts, gust fronts, etc.) that produce wind shear and turbulence hazardous to aircraft operations. The report consists of three parts: Part 1, Intercomparison of Wind Data from Airborne Lidar, Ground-Based Radars and Instrumented 444 m Tower; Part 2, The Structure of the Convective Atmospheric Boundary Layer as Revealed by Lidar and Doppler Radars; and Part 3, Doppler Lidar Observations in Thunderstorm Environments.

Bluestein, H. B.↗

Multi-Sensor Data from A-Train Instruments Brought Together for Atmospheric Research

The A-Train is comprised of a series of instruments, developed independently, that measure highly related atmospheric components along the same flight path. In order to intercompare data from this multitude of sensors, researchers must access, subset, visualize, analyze and correlate distributed atmosphere measurements from the various A-Train instruments. The A-Train Data Depot (ATDD) has been operational for over a year, successfully performing the aforementioned functions on behalf of researchers, thus providing co-registered data from the Cloudsat, CALIOP, AIRS, and MODIS instruments for further intercomparisons. Of late, significant data from OM1 and POLDER are now included in the 'depot'. By specifying the desired spatial and temporal range, the researcher can subset, visualize, co-register, and access multi-sensor A-Train data related to: Cloud, aerosol, atmospheric temperature, and water vapor parameters (vertical profile visualizations); Cloud Pressure, cloud top temperature, water vapor, cloud optical thickness, and aerosol products (horizontal strips subsetted +/- 100km from the profile visualizations), and; Cloud pressure parameters (2-D line plots overlayed on the vertical profiles). All data is plotted using the GIOVANNI data exploration tool. A new feature of GIOVANNI is its ability to have collocated and subsetted data sets as well as PNG image files downloaded to the researcher's computing facility. By providing a convenient way to visualize and acquire multi-sensor data, ATDD affords users more time and effort to further their research.

Smith, Peter M.↗

The epsilon Geminorum occultation - Evidence for waves or turbulence

Intercomparison of occultation data from various observatories shows poor correlation of the raw data but apparent correlations in the derived temperature profiles, as discussed in a paper by French and Elliott. We point out that the data are at least as consistent with Martian scintillation as they are with a semiglobal wave model.

Hubbard, W. B.↗

Analysis of lidar, radar and satellite measurements on severe thunderstorms and their environments

Intercomparison of wind data from the airborne Doppler lidar, ground-based Doppler radars, the 444 m NSSL-KTVY tower, and rawinsonde were completed. The vertical profile of wind in the PBL measured by the radars compared favorably with the profiles measured by the tower and rawinsonde while the one obtained from lidar data differed from the other three by as much as 3m/sec in wind speed and 38 degrees in direction. The time dependence of differences in wind estimates from radar and lidar suggested that these discrepencies could be attributed to a Schuler resonance in the aircraft's inertial navigation system which caused an erroneous component of the aircraft's velocity vector to be subtracted from the lidar radial velocities, thus creating errors in the synthesized wind speed and direction. The vertical profile of turbulent fluctuations of the horizontal wind detected by the different systems compared well. Also, spectra from the different sensing systems compared well in both magnitude and shape, suggesting that the lidar and radar detected similar turbulent structure.

Bluestein, H.↗

In-flight calibration of solar irradiance measurements by direct comparison with stellar observations

The Solstice instrument aboard the upper atmosphere research satellite (UARS) is designed to correct the deficiencies of both long-term instrument drift and of intercomparison of solar data sets taken at different times by different instruments. The Solstice will make repeated measurements, spaced throughout the mission, of a number of bright early-type stars. In addition, it will determine the ratio of the solar flux to the average flux from an ensemble of stars. This ratio will be monitored throughout the UARS mission and archived.

Rottman, Gary J.↗

NASA Giovanni Portals for NLDAS/GLDAS Online Visualization, Analysis, and Intercomparison

The North American Land Data Assimilation System (NLDAS) and Global Land Data Assimilation System (GLDAS) are generating a series of land surface forcing (e.g., precipitation, surface meteorology, and radiation), state (e.g., soil moisture and temperature, and snow), and flux (e.g., evaporation and sensible heat flux) products, simulated by several land surface models. To date, NLDAS and GLDAS have generated more than 30 (1979 - present) and 60 (1948 - present) years of data, respectively. To further facilitate data accessibility and utilization, three new portals in the NASA Giovanni system have been made available for NLDAS and GLDAS online visualization, analysis, and intercomparison.

Rui, Hualan↗

Special Observing Period (SOP) data for the Year of Polar Prediction site Model Intercomparison Project (YOPPsiteMIP)

The rapid changes occurring in the polar regions require an improved understanding of the processes that are driving these changes. At the same time, increased human activities such as marine navigation, resource exploitation, aviation, commercial fishing, and tourism require reliable and relevant weather information. One of the primary goals of the World Meteorological Organization's Year of Polar Prediction (YOPP) project is to improve the accuracy of numerical weather prediction (NWP) at high latitudes. During YOPP, two Canadian “supersites” were commissioned and equipped with new ground-based instruments for enhanced meteorological and system process observations. Additional pre-existing supersites in Canada, the United States, Norway, Finland, and Russia also provided data from ongoing long-term observing programs. These supersites collected a wealth of observations that are well suited to address YOPP objectives. In order to increase data useability and station interoperability, novel Merged Observatory Data Files (MODFs) were created for the seven supersites over two Special Observing Periods (February to March 2018 and July to September 2018). All observations collected at the supersites were compiled into this standardized NetCDF MODF format, simplifying the process of conducting pan-Arctic NWP verification and process evaluation studies. This paper describes the seven Arctic YOPP supersites, their instrumentation, data collection and processing methods, the novel MODF format, and examples of the observations contained therein. MODFs comprise the observational contribution to the model intercomparison effort, termed YOPP site Model Intercomparison Project (YOPPsiteMIP). All YOPPsiteMIP MODFs are publicly accessible via the YOPP Data Portal (Whitehorse: https://doi.org/10.21343/a33e-j150, Huang et al., 2023a; Iqaluit: https://doi.org/10.21343/yrnf-ck57, Huang et al., 2023b; Sodankylä: https://doi.org/10.21343/m16p-pq17, O'Connor, 2023; Utqiagvik: https://doi.org/10.21343/a2dx-nq55, Akish and Morris, 2023c; Tiksi: https://doi.org/10.21343/5bwn-w881, Akish and Morris, 2023b; Ny-Ålesund: https://doi.org/10.21343/y89m-6393, Holt, 2023; and Eureka: https://doi.org/10.21343/r85j-tc61, Akish and Morris, 2023a), which is hosted by MET Norway, with corresponding output from NWP models.

54 ENVIRONMENTAL SCIENCES↗

High Altitude Measurements of Radiance at High Spectral and Spatial Resolution for SIMBIOS Sensor Calibration, Validation, and Intercomparisons

The successful combination of data from different ocean color sensors depends on the correct interpretation of signal from each of these sensors. Ideally, the sensor measured signals are calibrated to geophysical units of spectral radiance, and sensor artifacts are removed and corrected. The calibration process resamples the signal into a common radiometric data space so that subsequent ocean color algorithms that are applied to the data are based on physical processes and are inherently sensor independent. The objective of this project is to calibrate and validate the on-orbit radiometric characteristics of Sea-Viewing Wide Field-of-view Sensor (SeaWiFS) with underflights of NASA's calibrated Airborne Visible/Infrared Imaging Spectrometer (AVIRIS). This objective is feasible because AVIRIS measures the same spectral range as SeaWIFS at higher spectral resolution. In addition to satellite sensor underflights, the AVIRIS project has supported comparison and analysis of the radiometric calibration standards used for AVIRIS and SeaWIFS. To date, both the OCTS and SeaWIFS satellite sensors have been underflown by AVIRIS with matching spectral, spatial, geometric, radiometric, and temporal domains. The calibration and validation objective of this project is pursued for the following reasons: (1) Calibration is essential for the quantitative use of SeaWIFS and other SIMBIOS (Sensor Intercomparison and Merger for Biological and Interdisciplinary Oceanic Studies) sensor data; (2) Calibration in the laboratory of spaceborne sensors is challenging; (3) Satellite sensors are subjected aging on the ground and to trauma during launch; (4) The Earth orbit environment is significantly different than the laboratory calibration environment; (5) Through years of effort AVIRIS has been demonstrated to be well calibrated; and (6) AVIRIS can match the spectral and spatial observation characteristics near the top of the atmosphere at the time of SeaWIFS measurements.

Green, Robert O.↗

Intercomparison of the Nimbus 7 SBUV/TOMS total ozone data sets with Dobson and M83 results

Two years of SBUV/TOMS (Solar Backscattered Ultraviolet/Total Ozone Mapping Spectrometer) data spanning the period November 1978 to October 1980 have been processed and compared with ground-based ozone measurements made at 62 Dobson spectrophotometer and 18 M83 filter photometer ozone stations. Results show that the satellite ozone values are consistently smaller than those measured by the stations; most of this difference is probably due to an error in the currently accepted ozone absorption cross sections at ultraviolet wavelengths. Dobson-derived ozone amounts agree extremely well with those derived from each of the satellite instruments. Long-term stability of the ozone derived by the two instruments is expected to be better than 0.5 percent per year.

Bhartia, P. K.↗