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At least 91 records · Page 5

An integrated Imaging and Modeling Toolbox for Accelerated Development of Root-focused Crops at Field Scales

This project, led by Yuxin Wu at the LBNL, was proposed to develop an integrated imaging-modeling toolbox allowing for accelerated development of root-focused crops at field scales. Our approach is based on a novel root phenotyping method we termed Tomographic Electrical Rhizosphere Imaging (TERI), which provides advanced phenotyping of key root traits by sensing the electrical impedance response of roots and soil to external electrical excitations, and mapping these responses to root and soil properties. The role of Noble Research Institute in this project is to assist TERI model establishment and validation by collecting various above- and below-ground traits data from container- and field-grown plants (mainly wheat). Over the last three years, Noble Research Institute has conducted 3 phases of experiments as proposed including: (Phase 1) greenhouse and hoop-house experiments in wheat and pecan plants grown under controlled container-grown conditions; (Phase 2) space-planted individual plant field experiment using different wheat varieties; and (Phase 3) small-plot planted field experiment using different wheat varieties, as well as using different small grains species including wheat, rye, triticale, oat and barley for increasing plant variation.

60 APPLIED LIFE SCIENCES↗

Evaluation of Fast-Time Wake Vortex Prediction Models

Current fast-time wake models are reviewed and three basic types are defined. Predictions from several of the fast-time models are compared. Previous statistical evaluations of the APA-Sarpkaya and D2P fast-time models are discussed. Root Mean Square errors between fast-time model predictions and Lidar wake measurements are examined for a 24 hr period at Denver International Airport. Shortcomings in current methodology for evaluating wake errors are also discussed.

Proctor, Fred H.↗

Comparison of plug flow and multi-node stratified tank modeling approaches regarding computational efficiency and accuracy

Residential water heaters contain water stratified by temperature-driven density differences. This implies that a water tank can reach a state in which the top and bottom sections have different temperatures, unless mixing happens. A high degree of thermal stratification can improve the efficiency of some water heaters, by saving the amount of energy required for the heat-up process. Studies of stratification became popular in the 1970s and it remains an active research topic today. The research has led to the development of different models and techniques to better predict and define a stratified tanks behavior. By comparing these models and techniques used previously to describe thermal stratification, the phenomenon could be better understood, exploited, and used to increase efficiency and thermal energy capacity in modern water tanks. From the existing models, we found the one-dimensional standard plug-flow and a multi node model to be appropriate for analyzing the processes of the heat up and cool-down in a water tank. These two models are based on energy balances. This work involved comparing the accuracy and computational effort needed to implement these models. To assess accuracy, we compared both types of existing models to experimental data (also collected in this work) which included a heat up process using an external heat pump. This external process included a layering process that has an eddy diffusivity at five times the rate of thermal diffusion. For this project, we implemented the models in MATLAB, the multi-paradigm numerical computing environment. We quantified model accuracy using the root mean squared error between modeled data and experimental data for six measured tank temperatures. Comparing the accuracy and the computational time taken to run the simulation provides a method to contrast the performance of each model and a way to rate it. The multi node model was run using from 6 to 96 spatial nodes; the plug flow model was run using 1 to 0.001 º C temperature bin sizes. Additionally, timesteps were varied from 4 to 236 s. The results quantify the tradeoff between accuracy and computational time, providing guidance for simulations to intelligently select the best model type and simulation parameters. This research can be used to validate the pre-existing models and possibly improve the modern water tank.

Bulnes, Fernando Karg↗

A generalized Lyapunov theory for robust root clustering of linear state space models with real parameter uncertainty

The problem of analyzing and designing controllers for linear systems subject to real parameter uncertainty is considered. An elegant, unified theory for robust eigenvalue placement is presented for a class of D-regions defined by algebraic inequalities by extending the nominal matrix root clustering theory of Gutman and Jury (1981) to linear uncertain time systems. The author presents explicit conditions for matrix root clustering for different D-regions and establishes the relationship between the eigenvalue migration range and the parameter range. The bounds are all obtained by one-shot computation in the matrix domain and do not need any frequency sweeping or parameter gridding. The method uses the generalized Lyapunov theory for getting the bounds.

Yedavalli, R. K.↗

Rays for Roots - Integrating Backscatter X-Ray Phenotyping, Modeling and Genetics to Increase Carbon Sequestration and Switchgrass Resource Use (Final Report)

To increase carbon (C) deposition in the soil and enhance crop resource use efficiency, characterizing root form and function is essential. Several root and soil traits have been linked to increased root-to-soil C transfer. Technology that could provide high-resolution characterization of many of these traits in field conditions would revolutionize our ability to study and understand how to increase C sequestration. In this effort, we developed an initial early prototype backscatter X-ray system for non-destructive imaging of root traits. We collected initial backscatter X-ray data in field and lab settings and carried out early analysis of these data. Along with this prototype, we also developed a suite of root phenotyping approaches including advanced minirhizotron image analysis, soil core imaging, and mesocosm imaging. Minirhizotron (MR) tubes are clear tubes inserted into the soil in the field and used to image roots and the surrounding soil. Our team has developed deep learning-based methods that can segment roots from soil that can learn from imprecise image-level labels. The ability to learn or fine-tune our deep learning algorithms from image-level labels allows easier and faster application of these approaches to new locations and new plant species. We have successfully implemented and applied our MR analysis approaches to thousands of switchgrass MR images collected across geographical regions. An advantage of MR imaging is the ability to collect root and soil images over time. Our soil core analysis included collecting hundreds of soil core samples from harvested switchgrass fields and imaging these cores with both X-ray CT and backscatter X-ray imaging. Initial segmentation approaches for the X-ray CT images of these cores have been developed and applied. An advantage of soil core analysis is that it preserves the three-dimensional structures of the roots and soil in the core collected. Our group also developed photogrammetry-based mesocosm root imaging and phenotyping approaches. In this approach, a plant was grown in a large mesocosm with a three-dimensional grid of thin supporting lines inserted throughout the mesocosm. After the plant (and, correspondingly, the root architecture is grown and established) the soil media was removed and the supporting lines approximately preserved the three-dimensional root architecture. Then, we applied photogrammetry techniques to create a three-dimensional digital representation of the root architecture for which we developed analysis algorithms including skeletonization. We carried out our phenotyping development with powerful switchgrass resources and physiological and agroecosystem modeling to deliver novel technology. This project contributes to multiple ARPA-E missions including reduction of foreign imports of energy, reduction of energy-related emissions including greenhouse gases, and ensuring that the United States maintains a technological lead in developing and deploying advanced energy technology. Furthermore, the developed tools could transform public and private plant breeding and could be broadly applicable to other crops and, potentially, other application areas. Our team of engineers, plant and soil scientists, and modelers i) developed an early prototype backscatter X-ray platform that can operate in field conditions; ii) developed a suite of root phenotyping and characterization approaches as described above; iii) developed and carried out plant biology and physiology roots studies and; iv) developed and implemented mechanistic physiological modeling.

42 ENGINEERING↗

Clover development during spaceflight: A model system

The development of legume root nodules was studied as a model system for the examination of gravitational effects on plant root development. In order to examine whether rhizobial association with clover roots can be achieved in microgravity, experiments were performed aboard the KC-135 parabolic aircraft and aboard the sounding rocket mission Consort 3. Binding of rhizobia to roots and the initial stages of root nodule development successfully occurred in microgravity. Seedling germination experiments were performed in the sliding block device, the Materials Dispersion Apparatus, aboard STS-37. When significant hydration of the seeds was achieved, normal rates of germination and seedling development were observed.

Guikema, James A.↗

An evaluation of the Neuber and Glinka relations for monotonic loading

The abilities of six models of notch root behavior are evaluated by comparing them with experimental results over a range of constraints. The models studied are the Neuber model, the Walker modified Neuber model, the Glinka models for plane strain and for plane stress, and two modifications of the Glinka models. Comparisons are made with strains measured at the roots of notches by resistance gages or by laser-based interferometry.

Sharpe, W. N., Jr.↗

Endophyte‐induced systemic spatial reprogramming of metabolism in Populus trichocarpa roots under drought

Beneficial, facultative endophytes help plants thrive in challenging environments by altering their host's metabolism, but how these cellular scale metabolic changes propagate to the systems biology scale is unknown. In this work, we employed a high-resolution chemical imaging approach to map metabolic changes at the Populus trichocarpa root-zone and cell-type levels combined with machine learning (ML) models to identify root metabolites and exudates that have predictive power over treatment class. We found that a nine-strain consortium of beneficial endophytes differentially altered the metabolome of droughted root tissues in a manner specific to cell type and root zone, with endophyte abundance showing a clear correlation to individual metabolites. Our study demonstrates that integrating spatial metabolomics with ML can reveal localized metabolic patterns linked to root–microbe interactions and generate novel hypotheses about underlying biological mechanisms.

Drought↗

On Convergence of Development Costs and Cost Models for Complex Spaceflight Instrument Electronics

Development costs of a few recent spaceflight instrument electrical and electronics subsystems have diverged from respective heritage cost model predictions. The cost models used are Grass Roots, Price-H and Parametric Model. These cost models originated in the military and industry around 1970 and were successfully adopted and patched by NASA on a mission-by-mission basis for years. However, the complexity of new instruments recently changed rapidly by orders of magnitude. This is most obvious in the complexity of representative spaceflight instrument electronics' data system. It is now required to perform intermediate processing of digitized data apart from conventional processing of science phenomenon signals from multiple detectors. This involves on-board instrument formatting of computational operands from row data for example, images), multi-million operations per second on large volumes of data in reconfigurable hardware (in addition to processing on a general purpose imbedded or standalone instrument flight computer), as well as making decisions for on-board system adaptation and resource reconfiguration. The instrument data system is now tasked to perform more functions, such as forming packets and instrument-level data compression of more than one data stream, which are traditionally performed by the spacecraft command and data handling system. It is furthermore required that the electronics box for new complex instruments is developed for one-digit watt power consumption, small size and that it is light-weight, and delivers super-computing capabilities. The conflict between the actual development cost of newer complex instruments and its electronics components' heritage cost model predictions seems to be irreconcilable. This conflict and an approach to its resolution are addressed in this paper by determining the complexity parameters, complexity index, and their use in enhanced cost model.

Kizhner, Semion↗

The UPR regulator IRE1 promotes balanced organ development by restricting TOR-dependent control of cellular differentiation in Arabidopsis

Proteostasis of the endoplasmic reticulum (ER) is controlled by sophisticated signaling pathways that are collectively called the unfolded protein response (UPR) and are initiated by specialized ER membrane-associated sensors. The evidence that complete loss-of-function mutations of the most conserved of the UPR sensors, inositol-requiring enzyme 1 (IRE1), dysregulates tissue growth and development in metazoans and plants raises the fundamental question as to how IRE1 is connected to organismal growth. To address this question, we interrogated the Arabidopsis primary root, an established model for organ development, using the tractable Arabidopsis IRE1 mutant ire1a ire1b, which has marked root development defects in the absence of exogenous stress. We demonstrate that IRE1 is required to reach maximum rates of cell elongation and root growth. We also established that in the actively growing ire1a ire1b mutant root tips the Target of Rapamycin (TOR) kinase, a widely conserved pro-growth regulator, is hyperactive, and that, unlike cell proliferation, the rate of cell differentiation is enhanced in ire1a ire1b in a TOR-dependent manner. By functionally connecting two essential growth regulators, these results underpin a novel and critical role of IRE1 in organ development and indicate that, as cells exit an undifferentiated state, IRE1 is required to monitor TOR activity to balance cell expansion and maturation during organ biogenesis.

59 BASIC BIOLOGICAL SCIENCES↗

An efficient computational method for characterizing the effects of random surface errors on the average power pattern of reflectors

Based on the works of Ruze (1966) and Vu (1969), a novel mathematical model has been developed to determine efficiently the average power pattern degradations caused by random surface errors. In this model, both nonuniform root mean square (rms) surface errors and nonuniform illumination functions are employed. In addition, the model incorporates the dependence on F/D in the construction of the solution. The mathematical foundation of the model rests on the assumption that in each prescribed annular region of the antenna, the geometrical rms surface value is known. It is shown that closed-form expressions can then be derived, which result in a very efficient computational method for the average power pattern. Detailed parametric studies are performed with these expressions to determine the effects of different random errors and illumination tapers on parameters such as gain loss and sidelobe levels. The results clearly demonstrate that as sidelobe levels decrease, their dependence on the surface rms/wavelength becomes much stronger and, for a specified tolerance level, a considerably smaller rms/wavelength is required to maintain the low sidelobes within the required bounds.

Rahmat-Samii, Y.↗

Test of model of equivalence of tree height growth and transpiration rates in percolation-based phenomenology for root-soil interaction

We recently introduced a phenomenological model to represent root-soil interactions that accounts for variability in tree growth on local scales and within species (Hunt et al., 2020). Here, using publicly available datasets, we examine the developed model to provide additional empirical evidence, which also gives novel insight into two particular tree species, Eucalyptus regnans and Eucalyptus sieberri. In particular, we discovered two novel aspects of the model: a power-law time dependence with a different power-law exponent than usually heretofore applied, and apparently a direct equivalence of results for transpiration and growth rates. Also, although our original expectation was that growth and transpiration were merely proportional, comparison of the entire BAAD data set (Falster et al., 2015) for tree height with our model (Hunt et al., 2020; Fig. 1) already suggested approximate equality of these disparate measurements (volume/area, height). In this present manuscript we find further evidence for equivalence of the two measurements and provide additional theoretical basis for a model assumption regarding constancy of sap flow rates.

54 ENVIRONMENTAL SCIENCES↗

Quantification of manganese for ChemCam Mars and laboratory spectra using a multivariate model

In this work, we report a new calibration model for manganese using the laser-induced breakdown spectroscopy instrument that is part of the ChemCam instrument suite onboard the NASA Curiosity rover. The model has been trained using an expanded set of 523 manganese-bearing rock, mineral, metal ore, and synthetic standards. The optimal calibration model uses the Partial Least Squares (PLS) and Least Absolute Shrinkage and Selection Operator (LASSO) multivariate techniques, with a novel “double blending” technique. We determined the detection limit for manganese is 82 ppm using a method blank procedure and is possibly as low as 27 ppm based on visual inspection of the spectra. Based on a representative test set consisting of measurements on 93 standards, the double blended multivariate model shows a Root Mean Squared Error of Prediction (RMSEP) accuracy of 1.39 wt% MnO for the full blended model. Employing a local RMSEP estimate where the model performance is evaluated based on nearby test samples, the accuracy is 0.03 wt% at the quantification limit (0.05 wt% MnO), 0.4 wt% accuracy at 1.0 wt% MnO, and 4.4 wt% accuracy at 100 wt% MnO. Precision is estimated using the standard deviation of the test set measurements, and is ±0.01 wt% MnO at the quantification limit, ±0.09 wt% MnO at 1.0 wt% MnO, and ± 2.1 wt% MnO at 100 wt% MnO (all 1 standard deviation). This new calibration is important for understanding the variation of manganese in the bedrock with the Curiosity rover on Mars, which provides insight into past redox conditions on Mars.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Super resolution for root imaging

Premise High‐resolution cameras are very helpful for plant phenotyping as their images enable tasks such as target vs. background discrimination and the measurement and analysis of fine above‐ground plant attributes. However, the acquisition of high‐resolution images of plant roots is more challenging than above‐ground data collection. An effective super‐resolution (SR) algorithm is therefore needed for overcoming the resolution limitations of sensors, reducing storage space requirements, and boosting the performance of subsequent analyses. Methods We propose an SR framework for enhancing images of plant roots using convolutional neural networks. We compare three alternatives for training the SR model: (i) training with non‐plant‐root images, (ii) training with plant‐root images, and (iii) pretraining the model with non‐plant‐root images and fine‐tuning with plant‐root images. The architectures of the SR models were based on two state‐of‐the‐art deep learning approaches: a fast SR convolutional neural network and an SR generative adversarial network. Results In our experiments, we observed that the SR models improved the quality of low‐resolution images of plant roots in an unseen data set in terms of the signal‐to‐noise ratio. We used a collection of publicly available data sets to demonstrate that the SR models outperform the basic bicubic interpolation, even when trained with non‐root data sets. Discussion The incorporation of a deep learning–based SR model in the imaging process enhances the quality of low‐resolution images of plant roots. We demonstrate that SR preprocessing boosts the performance of a machine learning system trained to separate plant roots from their background. Our segmentation experiments also show that high performance on this task can be achieved independently of the signal‐to‐noise ratio. We therefore conclude that the quality of the image enhancement depends on the desired application.

Ruiz‐Munoz, Jose F.↗

Flutter and Divergence Analysis using the Generalized Aeroelastic Analysis Method

The Generalized Aeroelastic Analysis Method (GAAM) is applied to the analysis of three well-studied checkcases: restrained and unrestrained airfoil models, and a wing model. An eigenvalue iteration procedure is used for converging upon roots of the complex stability matrix. For the airfoil models, exact root loci are given which clearly illustrate the nature of the flutter and divergence instabilities. The singularities involved are enumerated, including an additional pole at the origin for the unrestrained airfoil case and the emergence of an additional pole on the positive real axis at the divergence speed for the restrained airfoil case. Inconsistencies and differences among published aeroelastic root loci and the new, exact results are discussed and resolved. The generalization of a Doublet Lattice Method computer code is described and the code is applied to the calculation of root loci for the wing model for incompressible and for subsonic flow conditions. The error introduced in the reduction of the singular integral equation underlying the unsteady lifting surface theory to a linear algebraic equation is discussed. Acknowledging this inherent error, the solutions of the algebraic equation by GAAM are termed 'exact.' The singularities of the problem are discussed and exponential series approximations used in the evaluation of the kernel function shown to introduce a dense collection of poles and zeroes on the negative real axis. Again, inconsistencies and differences among published aeroelastic root loci and the new 'exact' results are discussed and resolved. In all cases, aeroelastic flutter and divergence speeds and frequencies are in good agreement with published results. The GAAM solution procedure allows complete control over Mach number, velocity, density, and complex frequency. Thus all points on the computed root loci can be matched-point, consistent solutions without recourse to complex mode tracking logic or dataset interpolation, as in the k and p-k solution methods.

Edwards, John W.↗

Measurement of Geopotential Heights by GPS Radio Occultation

Geopotential heights of constant pressure surfaces are retrieved from global positioning system (GPS) radio occultation data. In order to assess accuracy a subset of data obtained by GPS/MET during spring 1995 and summer 1995 are compared to the output of the European Centre for Medium-Range Weather Forecasts (ECMWF) global model. The root-mean-square measurement error is 20 m throughout the upper troposphere and lower stratosphere. Furthermore, the ECMWF global model contains enhanced errors in the southeast Pacific. In probing the data for potential utility in climate studies, a Bayesian interpolation technique is used to map the geopotential height fields in the upper troposphere during the summer. Despite limitations of the GPS/MET data set the global average 300-mbar geopotential height over a 2-week period in summer 1995 is determined with an accuracy of 7 m. By obtaining greater coverage and partially resolving synoptic variability, a future constellation of 16 orbiting receivers could obtain global average geopotential height estimates in the upper troposphere with an accuracy of 1 m each day. Accuracy would be somewhat worse for regional studies, except in the tropics where synoptic variability is depressed.

Leroy, Stephen S.↗

Root Foraging Alters Global Patterns of Ecosystem Legacy From Climate Perturbations

The response of terrestrial ecosystems to climate perturbations typically persist longer than the timescale of the forcing, a phenomenon broadly referred to as legacy. Understanding the strength of legacy is critical for predicting ecosystem sensitivity to climate extremes and the extent that persistent changes in surface-atmosphere exchange might feedback onto climate. The cause of ecosystem legacy has been associated with myriad factors such as changes in aboveground biomass, however, few studies have tested how changes in the depth distribution of fine roots in response to perturbation might alter an ecosystem's recovery time. We explore this question using an Earth System Model that includes a dynamic root module where vegetation can forage for water and nutrients by altering their root profiles. The global simulations presented here show that in response to water stress events most ecosystems deepen their root profiles. In semi-arid ecosystems, this response expedites recovery (i.e., less legacy) relative to simulations without dynamics roots because access to deeper water pools after the initial event remains favorable. In wetter ecosystems, the development of deeper root profiles slows down the recovery timescale (i.e., more legacy) because the deeper root profile reduces access to nutrients and is thus unfavorable. Here, the simulations show that while the inclusion of dynamic roots might only minimally affect global patterns of Gross Primary Productivity and Evapotranspiration, the shift in root profile alters the timescale of recovery. Studies interested in the sustained response of land surfaces fluxes to climate disturbances should consider models that include dynamic root capability.

54 ENVIRONMENTAL SCIENCES↗

Response Surface Modeling Using Multivariate Orthogonal Functions

A nonlinear modeling technique was used to characterize response surfaces for non-dimensional longitudinal aerodynamic force and moment coefficients, based on wind tunnel data from a commercial jet transport model. Data were collected using two experimental procedures - one based on modem design of experiments (MDOE), and one using a classical one factor at a time (OFAT) approach. The nonlinear modeling technique used multivariate orthogonal functions generated from the independent variable data as modeling functions in a least squares context to characterize the response surfaces. Model terms were selected automatically using a prediction error metric. Prediction error bounds computed from the modeling data alone were found to be- a good measure of actual prediction error for prediction points within the inference space. Root-mean-square model fit error and prediction error were less than 4 percent of the mean response value in all cases. Efficacy and prediction performance of the response surface models identified from both MDOE and OFAT experiments were investigated.

Morelli, Eugene A.↗